Consulta: subjectFacets:"Ovine"
Registros recuperados: 31
Data/hora: 10/06/2024 12:19:15

Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes
Provedor de dados: 74
Autores: Zaros,Lilian Giotto; Coutinho,Luiz Lehmann; Sider,Lúcia Helena; Medeiros,Henrique Rocha de; Neves,Maria Rosalba Moreira das; Benvenuti,Camila Loures; Navarro,Andrine Maria do Carmo; Vieira,Luiz da Silva.
Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Control genes; Gastrointestinal nematodes; Ovine; Real-time RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300018

Avaliação da biodiversidade da Caatinga para controle de nematódeos gastrintestinais de caprinos e ovinos.
Provedor de dados: 14
Autores: NOGUEIRA, D. M.
Na produção animal, a verminose é um dos fatores limitantes da caprinocultura, sendo considerada a principal doença responsável pelas elevadas perdas econômicas no rebanho. Associado a esse problema está o uso indiscriminado de drogas anti--helmínticas alopáticas que é responsável pela seleção de nematódeos resistentes ou de nematódeos que desenvolvem resistência aos anti-helmínticos. Esta atividade teve como objetivos testar diferentes dosagens em caprinos e/ou ovinos de plantas medicinais já citadas na literatura como detentoras de ação anti-helmíntica e avaliar a eficácia das plantas medicinais da Caatinga conhecidas popularmente como anti-helmínticas, porém ainda não citadas na literatura. Foram utilizados caprinos e ovinos mestiços para cada...
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Caprine; Ovine; Caprino; Ovino; Produção animal; Biodiversidade; Caatinga; Doença; Verminose.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/937157

Desempenho produtivo de ovinos da raça Santa Inês, no Acre.
Provedor de dados: 14
Autores: MAIA, M. da S.; DIAS, R. P.
Visando avaliar a adaptabilidade e determinar as médias de produção dos ovinos da raça Santa Inês, nas condições climáticas do Acre, foram adquiridos do Nordeste do Brasil 40 matrizes e dois reprodutores desta raça, os quais foram criados semi-extensivamente, tendo como base alimentar pastagem cultivada de Brachiaria humidicola (quicuio-da-amazônia). o sistema de acasalamento foi a monta natural a campo, onde o macho permaneceu com as fêmeas o ano todo. Após quatro anos de estudos, obteve-se os seguintes índices produtivos: taxa de fertilidade ao parto, 83,1%; prolificidade, 1,24 cordeiros nascidos/parto/ano; taxa de natalidade, 123%. A mortalidade de crias de zero a seis meses de idade foi 16,2%. Ocorreram parições durante todos os meses do ano, com uma...
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Ovine; Santa Inês; Acre; Oveja; Nutrición animal; Eficacia reproductora; Pastos forrajeros.; Pastizales; Ovino; Manejo; Eficiência reprodutiva; Nutrição animal; Pastagem; Gramínea forrageira; Capim Quicuio; Brachiaria humidicola; Sheep; Reproductive efficiency; Animal nutrition; Pastures; Forage grasses; Urochloa humidicola.
Ano: 1992
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/495394

SISTEMAS de produção misto para bovinocultura de corte e ovinocultura: microrregiões da Campanha e Lagoa Mirim-RS.
Provedor de dados: 14
bitstream/item/45669/1/SID-DOCUMENTOS-88-SISTEMAS-DE-PRODUCAO-MISTO-PARA-BOVINOCULTURA-DE-CORTE-E-OVINOCULTURA-CDU-636-2.pdf
Tipo: Sistema de Produção (INFOTECA-E)
Palavras-chave: Campanha; Lagoa Mirim; Rio Grande do Sul; Brasil; Production; System; Bovine; Ovine; Brazil.; Bovino; Gado de Corte; Ovino; Sistema de Produção.; Beef cattle.
Ano: 1977
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/905088

Digestibilidade "in vivo" dos constituintes da parede celular do capim quicuio-da-amazônia (Brachiaria humidicola (Rendle) Schweickerdt).
Provedor de dados: 14
Autores: CAMARAO, A. P.; BATISTA, H. A. M.; BRAGA, E.; DUTRA, S.
bitstream/item/40158/1/Circ-Tec-48-CPATU.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Capim quicuio-da-amazonia; Belém; Pará; Ovine; Feed; Grass.; Alimento; Animal; Brachiaria Humidicola; Digestibilidade; Gramínea; Ovino; Nutrição; Valor Nutritivo.; Digestibility; Nutritive value.; Nutrition.
Ano: 1984
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/383852

Identificação das espécies e a resistência de parasitos gastrintestinais de ovinos a anti-helmínticos em Mato Grosso do Sul.
Provedor de dados: 14
Autores: BIANCHIN, I.; MORAES, E. A. S. de; SILVA, K. F.; CATTO, J. B.; PAIVA, F.; HONER, M. R.
bitstream/item/139529/1/COT-108.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Parasitologia veterinária; Mato Grosso do Sul; Brasil; Ovine; Identification; Helmints.; Anti-Helmíntico; Helminto; Identificação; Ovino; Sanidade Animal.; Anthelmintics; Animal health; Brazil; Veterinary parasitology.
Ano: 2007
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/326655

Mucous Membrane of the Rumen of Ovines, Fed With Spineless, Forrage Cactus or Palm (Barbary Fig) (Opuntia ficus indica Mil): Hystochemical Study by Means of Light Microscopy
Provedor de dados: 77
Autores: Neiva,Gentileza Santos Martins; da Mota,Diógenes Luís; Batista,Ângela Maria Vieira; Sousa-Rodrigues,Célio Fernando de.
The mucosa of the rumen performs the functions of absorption, transportation, metabolism and protection, due to the presence of microorganisms in this compartment of the digestive tract, which are responsible for the digestion of approximately 60% of the organic material apparently digested. The histological structure and the histochemical profile of glicoconjugate of the mucosa of the stomach of sheep that were being fed with forrage cactus or palm (Giant Palm - Opuntia ficus indica Mil)1. Twelve sheep were used, with approximately 14 months old, which were oddly distributed in four diets: D1- 60.0% forrage palm + 40.0% concentrated ration; D2- 47.5% forrage palm + 12.5% elephant, napier grass + 40.0% concentrated ration, and D3 - 34,8% forrage palm +...
Tipo: Journal article
Palavras-chave: Mucous membrane; Histology; Histochemistry; Forrage palm; Forrage cactus; Ovine.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500034

USE OF FIBRIN GLUE DERIVED FROM SNAKE VENOM IN TESTICULAR BIOPSY OF RAMS
Provedor de dados: 79
Autores: SARTORI FILHO,R.; PRESTES,N. C.; THOMAZINI,I. A.; MENDES-GIANNINI,M. J.; TOSCANO,E.; CANAVESSI,A. M. O.; BARRAVIERA,B.
Sequelae due to testicular biopsy such as hemorrhage, adhesion and fibrosis may be limiting factors to the use of this surgical procedure. Fibrin glue (FG) derived from snake venom was used to minimize these sequelae, as well as to evaluate its healing property in tunica vaginalis and scrotal skin of rams. Applicability of fibrin glue derived from snake venom was tested in different tissues of other animals such as in sciatic nerve and colon of rats and skin of rabbits. In the present study, 30 healthy adult rams were used. They were divided into 3 groups of 10 animals each as follows: G1: fibrin glue group (application of fibrin glue on puncture sites and skin incisions after bilateral testicular biopsy with a Tru-Cut needle); G2: swab/nylon group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin tecidual adhesive; Snake venoms; Biopsy; Testicle; Ovine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100003

Leptospira spp. infection in sheep herds in southeast Brazil
Provedor de dados: 80
Autores: Barbante,Priscila; Shimabukuro,Fabio H; Langoni,Helio; Richini-Pereira,Virgínia B; Lucheis,Simone B.
Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Ovine; Serology; Culture; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325

Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)
Provedor de dados: 80
Autores: Netto,D. P.; Chiacchio,S. B.; Bicudo,P. L.; Alfieri,A. A.; Balarim,M. R. S.; Nascimento,N.
Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematology; Gamma radiation; Irradiated crotalic venom; Crotalic antiserum; Ovine; Crotalus durissus terrificus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100004

Productive and reproductive performances of Santa Inês ewes fed diets supplemented with protected fat in the postpartum
Provedor de dados: 86
Autores: Santos,Marcos Paulo dos; Godoy,Marcelo Marcondes de; Sousa,Cleber Luiz de; Assis,Roberta de Moura; Sena,Cassius Vinicius Barbosa.
Abstract: The objective of this work was to evaluate the effect of the inclusion of protected fat in the concentrate on the productive and reproductive performances of Santa Inês ewes in feedlot, up to 60 days after parturition. Thirty-six pregnant Santa Inês ewes, with an average age of 24 months and initial weight of 46.54±7.87 kg, were used. A completely randomized experimental design was used, with three treatments: CS, concentrated supplement; G5, concentrated supplement with 5.5% protected fat (PF); and G10, concentrated supplement with 13.5% PF. The productive performance parameters were evaluated every two weeks. At 60 days after parturition, the chemical composition of milk and the reproductive parameters were determined. The inclusion of PF in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body weight; Ovine; Polyunsaturated fatty acids; Supplementation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000700548

Resultados de Entrevistas a los Restantes Actores de la Cadena Cárnica Ovina.
Provedor de dados: 31
Autores: Lanfranco,   Bruno; Robaina,   Ricardo; Luzardo,   Santiago.
Resultados de la 1ra Auditoría de Calidad de la Carne Ovina. Uruguay
Tipo: Book
Palavras-chave: Quality audit; Meat quality; Quality assurance; Meat-packing industry; Ovine; Supply chain; Lamb; Food Consumption/Nutrition/Food Safety; Industrial Organization; Livestock Production/Industries; Production Economics.
Ano: 2003
URL: http://purl.umn.edu/197554

Effectiveness of a low-dose Norgestomet ear implant in short-term protocols to induce estrus in ewes during the non-breeding season in Brazil.
Provedor de dados: 96
Autores: Lima Júnior,Dayrton Farias; Barreto,José Victor Pronievicz; Sterza,Fabiana de Andrade Melo; Souza-Cáceres,Mirela Brochado; Pontes,Vanessa Pereira; Zundt,Marilice; Castilho,Caliê; Cunha Filho,Luiz Fernando Coelho da.
ABSTRACT The objective of this study was to evaluate the reproductive performance of ewes subjected to a short-term protocol for estrus induction using 1 mg of Norgestomet during the non-breeding season in Brazil. A total of 84 ewes were randomly divided into two equal groups (n = 42 per group). Controlled Internal Drug Release (CIDR) containing 330 mg progesterone was inserted into the vagina in the first 42 ewes, and for the other 42 ewes in the second group, 1 mg of Norgestomet was placed subcutaneously in the convex surface of the ear. Implants and devices were removed in both groups on day 6. Following the removal of implants and devices, 10 mg of PGF2α and 300 IU of eCG were injected in all the ewes. After 48 h of application, three fertile rams were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Animal breeding; Ovine; Progesterone; Reproductive traits; Sheep; Synchronization of estrus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100603

Biometry and ultrasound evaluation of testicles and accessory glands in Santa Ines rams
Provedor de dados: 96
Autores: Ribeiro,Mariana da Silva; Quirino,Celia Raquel; Bartholazzi Junior,Aylton; Pacheco,Aline.
ABSTRACT This work aimed to investigate the relationship between echogenicity, testicular biometry, and accessory gland biometry and evaluate the echogenicity pattern of these structures in Santa Ines rams. Fifty-four healthy Santa Ines sheep were classified into three age groups: group 1 - 3 to 5 months (pre-pubertal); group 2 - 7 to 11 months (pubertal); and group 3 - 13 to 28 months (sexually mature). The averages of testicular biometry and bulbourethral gland biometry were different among age groups. The testicular parenchyma was homogeneous with low echogenicity (pre-pubertal group) and medium echogenicity (pubertal and sexually mature groups), suggesting that testicular echogenicity can be a helpful tool to identify the start of puberty. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine; Semen; Testicle; Ultrasonography.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000400317

Impact of total substitution of corn for soybean hulls in diets for lambs
Provedor de dados: 96
Autores: Bastos,Milena Patricia Viana; Carvalho,Gleidson Giordano Pinto de; Pires,Aureliano José Vieira; Silva,Robério Rodrigues da; Carvalho,Bruna Mara Aparecida de; Brandão,Kelly Couto; Maranhão,Camila Maida de Albuquerque.
The experiment was conducted to assess the effect of substituting corn for soybean hulls on the apparent digestibility of nutrients, carcass performance and characteristics, and yield of cuts of 25 non-castrated male Santa Ines lambs with an initial body weight of 20±2 kg, at approximately 6 months of age, sheltered individually in stalls (1.10 × 1.0 m), considering a totally randomized design, fed 600 g/kg of forage and 400 g/kg of concentrate. Soybean hulls replaced corn at 0, 250, 500, 750, and 1,000 g/kg of dry matter. The intake of dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), NDF corrected for ash and protein (NDFap), total carbohydrates (TC), non-fibrous carbohydrates (NFC) and total digestible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Average daily gain; Intensive system; Meat production; Ovine.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000300083

Variants in GH, IGF1, and LEP genes associated with body traits in Santa Inês sheep
Provedor de dados: 63
Autores: Machado,Alessandro Lima; Meira,Ariana Nascimento; Jucá,Adriana de Farias; Azevedo,Hymerson Costa; Muniz,Evandro Neves; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista.
ABSTRACT: Growth hormone (GH), insulin-like growth factor 1 (IGF1), and leptin (LEP) can be candidate genes for association studies because they play vital roles in the metabolism process. Thus, this study aimed to identify variants in these genes associated with body traits in Santa Inês sheep. The following were recorded: body weight at 100 (BW100) and 240 days (BW240), average daily gain (ADG), withers (WH) and croup (CH) heights, body length (BL), thoracic (TG) and leg (LG) girths, thoracic (TW) and croup (CW) widths, body depth (BD), rib eye area (REA), fat thickness (FT), and carcass finishing score (CFS). Single-locus association analysis was performed with 11 variants in IGF1, 18 in LEP, and 16 in GH. Moreover, two haplotypes in IGF1 and one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hormone; Carcass; Growth; Haplotype; Ovine.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000300401

Influência da monensina e levedura sobre parâmetros ruminais e metabólicos em cordeiros semiconfinados - DOI: 10.4025/actascianimsci.v30i2.4687
Provedor de dados: 2
Autores: Tabeleão, Vinícius Coitinho; UFPEL; Del Pino, Francisco Augusto Burcket; UFPEL; Goulart, Maikel Alan; UFPEL; Schwegler, Elizabeth; UFPEL; Moura, Sandra Vieira; UFPEL.
O objetivo deste estudo foi verificar a influência dos suplementos monensina e levedura sobre parâmetros ruminais e metabólicos de cordeiros semiconfinados. Foram utilizados 30 animais divididos em três grupos: controle (sem suplemento), ionóforo (500 g t-1  de Rumensin&amp;reg; ) e levedura (500 g t-1   de Beefsac&amp;reg; ).  Os animais foram mantidos em sistema de semiconfinamento, recebendo ração concentrada equivalente a 2% do peso corporal. O fluido ruminal foi coletado a cada sete dias, a fim de determinar o pH, potencial redox, tempo de sedimentação e flutuação, cor, odor, consistência e movimentação de protozoários. O mesmo intervalo foi adotado para coleta de sangue, para determinação de glucose, ureia, colesterol total, triacilgliceróis,...
Palavras-chave: 5.04.03.00-1    Nutrição e Alimentação Animal probiótico; Ionóforo; Perfil metabólico; Perfil ruminal; Ovinos. probiotic; Oonophore; Metabolic profile; Ruminal profile; Ovine.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4687

Animal performance and carcass characteristics from confined lambs fed on concentrate feed and additives
Provedor de dados: 42
Autores: Prado,Tayrone F.; França,Aldi F.S.; Meirinhos,Maria Lúcia G.; Peron,Hugo J.M.C.; Ferreira,Reginaldo N.; Oliveira,Leonardo G.; Corrêa,Daniel S.
ABSTRACT  The number of sheep flocks in Brazil is increasing. It is known that lambs must be slaughtered when young for producing quality meat. The current study evaluated the inclusion of protected methionine, protected lysine, lysophospholipid and amylolytic enzymes in a diet to lambs and their effects on weight gain and quantitative carcass traits at slaughtering. Eighty non-castrated male crossbred Dorper x Santa Inês lambs, 20.57 ± 4.33 kg live weight, were used. The feedlot lasted 64 days and 60 animals were slaughtered. There were no differences for live weight, daily feed intake, feed conversion and average daily weight gain at the first 28 days of feedlot. From the 28th day lysophospholipid treatment presented the highest live weight....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylolytic enzyme; Lysine; Lysophospholipid; Methionine; Ovine.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502255

Real-time PCR for detection of Brucella ovis and Histophilus somni in ovine urine and semen
Provedor de dados: 45
Autores: Moustacas,V.S.; Silva,T.M.A.; Costa,É.A.; Costa,L.F.; Paixão,T.A.; Santos,R.L.
RESUMO A epididimite infecciosa ovina é uma das principais enfermidades reprodutivas de carneiros. O presente estudo teve por objetivo desenvolver protocolos de PCR em tempo real para B. ovis e H. somni e avaliar sua aplicabilidade em amostras de sêmen e urina de carneiros. Delinearam-se primers e sondas espécie-específicos para cada agente. As sondas foram delineadas com o sistema TaqMan incorporando um marcador FAM para B. ovis e Cy5 para H. somni na extremidade 5' e um quencher na extremidade 3'. A PCR em tempo real para B. ovis e H. somni foi altamente sensível, uma vez que a amplificação de DNA ocorreu com até 0,2ng de DNA/reação. A especificidade dos iniciadores e sondas foi avaliada com amostras de DNA de outros agentes causadores de epididimite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious epididymitis; Ovine; Diagnosis; PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601751

Comparison of two surgical approaches for Laparoscopic Ovum Pick Up in Ewes
Provedor de dados: 45
Autores: Mariano,R.S.G.; Uscategu,R.A.R.; Nociti,R.P.; Barros,F.F.P.C.; Santos,V.J.C.; Coutinho,L.N.; Padilha-Nakaghi,L.C.; Silva,P.D.A.; Teixeira,P.P.M.; Vicente,W.R. R.
ABSTRACT The present study had as an aim to evaluate a right lateral access as an alternative method to laparoscopic ovum pick-up (LOPU) in sheep. Twenty-four Santa Ines ewes were randomly assigned in two groups with twelve animals each: RLD - positioned in right lateral decubitus, with 10º head-down tilt; and DD - positioned in dorsal decubitus with 35º head-down tilt. The following parameters were evaluated every 10 minutes during the procedure: total surgical time (ST), visualized follicles (VF), aspirated follicles (AF), recovered oocytes (RO), mean arterial pressure (MAP), heart rate (HR), respiratory rate (fR) and end tidal CO2 pressure (EtCO2). Pre and postoperative arterial hemogasometry parameters (PaO2, PaCO2, pH, CHCO3 and BE) were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine; Surgery; Biotechnology; Genitourinary.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300848

Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen
Provedor de dados: 45
Autores: Bergstein,T.G.; Bicudo,L.C.; Rodello,L.; Weiss,R.R.; Bicudo,S.D.
ABSTRACT Frozen and thawed ovine semen undergo morphological and functional changes that prevent or decrease the efficiency of fertilization. Sperm selection methods seek to improve the quality and viability of the fertilizing materials. Four sperm selection methods were employed, using two silica colloidal solutions coated with silane (silica colloidal-silane) or by polyvinylpyrrolidone (silica colloidal-PVP), and varying the volume of colloidal solution. Sperm kinematic and sperm recovery were evaluated by means of CASA. The protocols using silica colloidal-silane showed higher total motility (TM), progressive motility (PM) and percentage of rapid sperm (%RAP) compared to the methods employing silica colloidal-PVP and to the samples prior to sperm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine; Semen; Silane; Selection; Colloidal silica.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601539

Simultaneous assessment of plasmatic, acrosomal, and mitochondrial membranes in ram sperm by fluorescent probes
Provedor de dados: 45
Autores: Celeghini,E.C.C.; Nascimento,J.; Raphael,C.F.; Andrade,A.F.C.; Arruda,R.P.
In this experiment, it was defined a protocol of fluorescent probes combination: propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA), and JC-1. For this purpose, four ejaculates from three different rams (n=12), all showing motility &amp;gt;80% and abnormal morphology &lt;10%, were diluted in TALP medium and split into two aliquots. One of the aliquots was flash frozen and thawed in three continuous cycles, to induce damage in cellular membranes and to disturb mitochondrial function. Three treatments were prepared with the following fixed ratios of fresh semen:flash frozen semen: 0:100 (T0), 50:50 (T50), and 100:0 (T100). Samples were stained in the proposal protocol and evaluated by epifluorescence microscopy....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine; Semen; Epifluorescence microscopy; Sperm membranes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300006

Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
Provedor de dados: 45
Autores: Olivares,C.C.S.; Souza-Fabjan,J.M.G.; Fonseca,J.F.; Saraiva,H.F.R.A.; Côrtes,L.R.; Alfradique,V.A.P.; Balaro,M.F.A.; Oliveira,R.V.; Brandão,F.Z.
ABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P&gt; 0.05) among protocols. VCL (µm/s) was higher (P&lt; 0.05) after MP-II,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sperm capacitation; Frozen-thawed sperm; Ovine; Percoll protocols.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455

Distribution of selenium in sheep treated with dipheny diselenide
Provedor de dados: 45
Autores: Leal,M.L.R.; Rocha,J.B.T.; Corte,C.L.D.; Aires,A.R.; Rocha,J.F.X.; Zanatta,R.S.; Carpes,J.L.S.; Szinwelski,G.A.; Stefanello,S.; Pivoto,F.L.
ABSTRACT The aim of the present study was to report the in vivo distribution of selenium in sheep. For this, animals were allocated into two groups (control group and treated group) and kept in metabolic cages for a period of 37 days. The treated group received a single dose (6µmol/kg) of Diphenyl Diselenide, intravenously. Plasma and erythrocytes samples were collected at different times. Adipose tissue, muscles (latissimusdorsi, semitendinosus, and supra-scapular) heart, liver, lung, kidney, intestine and brain were sampled at 30 days post-treatment, in order to determine the selenium concentration. The results demonstrated that the selenium, from the Diphenyl Diselenide group, was higher in erythrocytes (4.8mg/L, six hours post-treatment) when compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Ovine; Selenium; Tissue.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401017

Validation of a PCR Assay for Chlamydophila abortus rRNA gene detection in a murine model
Provedor de dados: 52
Autores: Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Negrão,Fábio Juliano; Lunardi,Michele; Freitas,Julio Cesar de.
Chlamydophila abortus (C. abortus) is associated with reproductive problems in cattle, sheep, and goats. Diagnosis of C. abortus using embryonated chicken eggs or immortalized cell lines has a very low sensitivity. Polymerase chain reaction (PCR) assays have been used to detect C. abortus infection in clinical specimens and organ fragments, such as placenta, fetal organs, vaginal secretions, and semen. The aim of this study was to develop a PCR assay for the amplification of an 856-bp fragment of the rRNA gene of the Chlamydiaceae family. The PCR assay was evaluated using organs from 15 mice experimentally infected with the S26/3 reference strain of C. abortus. The results of the rRNA PCR were compared to the results from another PCR system (Omp2 PCR) that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Ovine; Caprine; Reproductive failures; Chlamydophila abortus; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700014

Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil
Provedor de dados: 58
Autores: Alves,Clebert José; Figueiredo,Salomão Moreira de; Azevedo,Sérgio Santos de; Clementino,Inácio José; Keid,Lara Borges; Vasconcellos,Sílvio Arruda; Batista,Carolina de Sousa Américo; Rocha,Vivianne Cambuí Mesquita; Higino,Severino Silvano.
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella ovis; Isolation; PCR; Ovine; Paraíba State.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016

Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil
Provedor de dados: 58
Autores: Oliveira,Rosângela C.; Yamaguti,Maurício; Marques,Lucas M.; Buzinhani,Melissa; Rizzo,Huber; Meira-Junior,Enoch Brandão de Souza; Guimarães,Ana Márcia S.; Gregory,Lilian; Timenetsky,Jorge.
Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ureaplasma; Ovine; Caprine; Molecular characterization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300560

Performance of confined and grazing lambs fed diets with different mineral-concentrate supplements
Provedor de dados: 118
Autores: Silva,Aderbal M. de A; Bezerra,Leilson R; Rufino,Sara R. M; Filho,José M. Pereira; Freire,Juliana M; da Silva,Divan S; Oliveira,Ronaldo L; Bayão,Geraldo F. V.
For confined animals, performance is improved when their diet is supplemented with a mineral mixture and protein energy. This study evaluated the effect of dietary supplementation on feedlot sheep on native pasture. Two experiments were conducted in sequence, with the animals constrained in one and animals confined to pasture in the other. The experiments were conducted with 32 Santa Ines lambs, with average initial weights of 16.8 and 19.9 kg for the constrained and pasture studies, respectively. In both experiments, the animals were offered the following supplements: Supplement 1 = 200 g of the concentrate without mineral mixture; Supplement 2 = 200 g of the concentrate with mineral mixture + Mn + Zn; Supplement 3 = 300 g of the concentrate with mineral...
Tipo: Journal article
Palavras-chave: Dry matter; Feed conversion; Manganese; Ovine; Zinc.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000300001

Single-port laparoscopic ovariectomy using a pre-tied loop ligature in Santa Ines ewes
Provedor de dados: 65
Autores: Barros,Felipe Farias Pereira da Câmara; Teixeira,Pedro Paulo Maia; Silva,Marco Augusto Machado; Coelho,Cassia Maria Molinaro; Lopes,Maristela de Cassia Seudo; Kawanami,Aline Eyko; Chung,Denise Granato; Coutinho,Leandro Nassar; Ribeiro,Rachel Bittencourt; Padilha,Luciana Cristina; Vicente,Wilter Ricardo Russiano.
ABSTRACT: The aim of the study was to develop and assess the feasibility, postoperative pain and inflammatory response of the single-port laparoscopic ovariectomy in ewes, using a simple pre-tied loop ligature technique. Pre-tied Meltzer's knot was employed for prophylactic hemostasis of the ovarian pedicle. Slipknot was inserted within the abdominal cavity through a 14-gauge needle and tied surrounding the ovarian pedicle. Mean surgical time, manipulation, ligature and resection of each ovary and anesthesia time were 63±20, 20±10 and 91±26 minutes, respectively. No bleeding occurred during the surgeries. Ewes showed low scores pain (0.5±0.5) at all time-points. Postsurgical plasma fibrinogen was within the normal range for sheep specie at all time-points....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laparoscopy; Ovary; Ovine; Single-site access; Slipknot.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001102033

Epidemiological, clinical and diagnostic aspects of sheep conidiobolomycosis in Brazil
Provedor de dados: 65
Autores: Weiblen,Carla; Pereira,Daniela Isabel Brayer; Dutra,Valéria; Godoy,Isabela de; Nakazato,Luciano; Sangioni,Luís Antonio; Santurio,Janio Morais; Botton,Sônia de Avila.
ABSTRACT: Conidiobolomycosis is an emerging disease caused by fungi of the cosmopolitan genus  Conidiobolus . Particular strains of  Conidiobolus coronatus, Conidiobolus incongruus and  Conidiobolus lamprauges , mainly from tropical or sub-tropical origin, cause the mycosis in humans and animals, domestic or wild. Lesions are usually granulomatous and necrotic in character, presenting two clinical forms: rhinofacial and nasopharyngeal. This review includes the main features of the disease in sheep, with an emphasis on the epidemiology, clinical aspects, and diagnosis of infections caused by Conidiobolus spp. in Brazil. In this country, the disease is endemic in the Northeast and Midwest, affecting predominantly woolless sheep breeds and occasioning death...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungal infection; Ovine; Zygomycosis; Conidiobolus spp .
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500839

Pregnancy diagnosis, fetal quantification and gender estimation by ultra-sonography in ewes
Provedor de dados: 64
Autores: Dias,Lilian Mara Kirsch; Souza,José Camisão de; Assis,Roberta de Moura; Raymundo,Camila de Moraes.
The objective of this experiment was to evaluate the accuracy of gestation, fetal sexing and quantification diagnoses in ewes. Pregnancy and fetal quantification were diagnosed in 105 ewes at 35 days of pregnancy. For the fetal gender diagnosis sexing diagnose 55 ewes between 49 and 59 days of pregnancy were used. All exams were recorded on DVD for posterior analysis. After birth, lamb sex was recorded to determine fetal sexing precision. Data were analyzed by chisquare (χ2) or Fisher's test, with a significance of 0.05. One hundred percent of pregnancy ultrasound diagnoses were correct. As for the fetal quantification diagnoses, there was an error of 12%. It was possible to diagnose the fetal sex in 87% of the 69 examined fetuses, and 90% of these were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine; Fetal sexing; Pregnancy diagnosis; Ultra-sonography.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000300035