Consulta: subjectFacets:"Pilocarpine"
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Data/hora: 03/06/2024 05:12:14

Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine
Provedor de dados: 97
Autores: LÓPEZ-SOLÍS,REMIGIO; PUENTE,MARGARITA; DURÁN,VIVIANA; MORALES-BOZO,IRENE; KEMMERLING,ULRIKE; PARDO,RODRIGO; WENK,CAROLINA.
Mouse salivary secretion provoked by single or multiple oral administrations of the cholinergic agent pilocarpine was characterized. An accessory ad hoc device, manipulated by a single operator, was used to collect saliva from various mice simultaneously. A single challenge by pilocarpine in the range 40-400 µg provoked a dose-dependent secretory response. The secretory response lasted for about 40 minutes and was constituted by four clearly defined stages, namely: lag (5-10 min), maximal flow (10 min), slowering (15-20 min) and postsecretory rest. In this response, usual parameters were: maximal flow rate, 30-40 µl/min; total volume of saliva, 250-350 µl/mouse and total salivary protein, about 700 µg/mouse. Temporal desensitization of the secretory...
Tipo: Journal article
Palavras-chave: Mouse; Saliva; Secretion; Pilocarpine; Polypeptides.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000023

Branch regeneration of jaborandi submitted to diferentes types of pruning and cropping systems
Provedor de dados: 109
Autores: Alves-Lameira,Osmar; Santos-Ribeiro,Fernanda Naiara; Teixeira-Rocha,Tainá; Mesquita-Germano,Carolina; Alves de Assis,Rafael Marlon.
Abstract The leaf of the jaborandi (Pilocarpus microphyllus) is the part of the plant of economic interest, from which the pilocarpine is extracted, therefore, leaf production is the main variable that determines the productivity of this species. Pilocarpus leaf production can be managed during its development phase. Thus, the objective of the present study was to evaluate branch regeneration of jaborandi submitted to two types of cutting/pruning and different cropping systems. Prunings were carried out in jaborandi plants from different accessions from the Janorandi Active Germoplasm Bank (BGA), that were under different cropping systems, full sun and shade. With pruning shears, cuts were made 10 and 20 cm from the branch bifurcations. The results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Accessions; Full sun; Pilocarpus microphyllus; Pilocarpine; Shade.
Ano: 2021
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122021000300285

The pilocarpine model of epilepsy: what have we learned?
Provedor de dados: 42
Autores: Scorza,Fulvio A.; Arida,Ricardo M.; Naffah-Mazzacoratti,Maria da Graça; Scerni,Débora A.; Calderazzo,Lineu; Cavalheiro,Esper A.
The systemic administration of a potent muscarinic agonist pilocarpine in rats promotes sequential behavioral and electrographic changes that can be divided into 3 distinct periods: (a) an acute period that built up progressively into a limbic status epilepticus and that lasts 24 h, (b) a silent period with a progressive normalization of EEG and behavior which varies from 4 to 44 days, and (c) a chronic period with spontaneous recurrent seizures (SRSs). The main features of the SRSs observed during the long-term period resemble those of human complex partial seizures and recurs 2-3 times per week per animal. Therefore, the pilocarpine model of epilepsy is a valuable tool not only to study the pathogenesis of temporal lobe epilepsy in human condition, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippocampus; Pilocarpine; Temporal lobe epilepsy; Rat.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300003

Absence-like seizures in adult rats following pilocarpine-induced status epilepticus early in life
Provedor de dados: 56
Autores: Ferreira,B.L.C.; Valle,A.C.; Cavalheiro,E.A.; Timo-Iaria,C.
Administration of pilocarpine causes epilepsy in rats if status epilepticus (SE) is induced at an early age. To determine in detail the electrophysiological patterns of the epileptogenic activity in these animals, 46 Wistar rats, 7-17 days old, were subjected to SE induced by pilocarpine and electro-oscillograms from the cortex, hippocampus, amygdala, thalamus and hypothalamus, as well as head, rostrum and vibrissa, eye, ear and forelimb movements, were recorded 120 days later. Six control animals of the same age range did not show any signs of epilepsy. In all the rats subjected to SE, iterative spike-wave complexes (8.1 ± 0.5 Hz in frequency, 18.9 ± 9.1 s in duration) were recorded from the frontal cortex during absence fits. However, similar spike-wave...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Absence seizures; Spike-wave; Status epilepticus; Pilocarpine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200010

Participation of the cholinergic system in the ethanol-induced suppression of paradoxical sleep in rats
Provedor de dados: 56
Autores: Papale,L.A.; Andersen,M.L.; Perry,J.C.; Tufik,S.
Sleep disturbance is among the many consequences of ethanol abuse in both humans and rodents. Ethanol consumption can reduce REM or paradoxical sleep (PS) in humans and rats, respectively. The first aim of this study was to develop an animal model of ethanol-induced PS suppression. This model administered intragastrically (by gavage) to male Wistar rats (3 months old, 200-250 g) 0.5 to 3.5 g/kg ethanol. The 3.5 g/kg dose of ethanol suppressed the PS stage compared with the vehicle group (distilled water) during the first 2-h interval (0-2 h; 1.3 vs 10.2; P &lt; 0.001). The second aim of this study was to investigate the mechanisms by which ethanol suppresses PS. We examined the effects of cholinergic drug pretreatment. The cholinergic system was chosen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholinergic; Atropine; Pilocarpine; Ethanol; Paradoxical sleep; Electroencephalography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900007

Neo-Timm staining in the thalamus of chronically epileptic rats
Provedor de dados: 56
Autores: Hamani,C.; Paulo,I. de; Mello,L.E.A.M.
The thalamus is an important modulator of seizures and is severely affected in cholinergic models of epilepsy. In the present study, chronically epileptic rats had their brains processed for neo-Timm and acetylcholinesterase two months after the induction of status epilepticus with pilocarpine. Both controls and pilocarpine-treated animals presented neo-Timm staining in the anterodorsal nucleus, laterodorsal nucleus, reticular nucleus, most intralaminar nuclei, nucleus reuniens, and rhomboid nucleus of the thalamus, as well as in the zona incerta. The intensity of neo-Timm staining was similar in control and pilocarpine-treated rats, except for the nucleus reuniens and the rhomboid nucleus, which had a lower intensity of staining in the epileptic group. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Pilocarpine; Zinc; Sprouting; Nucleus reuniens; Midline thalamus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100016

Anticonvulsant and proconvulsant roles of nitric oxide in experimental epilepsy models
Provedor de dados: 56
Autores: Del-Bel,E.A.; Oliveira,P.R.; Oliveira,J.A.C.; Mishra,P.K.; Jobe,P.C.; Garcia-Cairasco,N.
The effect of acute (120 mg/kg) and chronic (25 mg/kg, twice a day, for 4 days) intraperitonial injection of the nitric oxide (NO) synthase (NOS) inhibitor NG-nitro-L-arginine (L-NOARG) was evaluated on seizure induction by drugs such as pilocarpine and pentylenetetrazole (PTZ) and by sound stimulation of audiogenic seizure-resistant (R) and audiogenic seizure-susceptible (S) rats. Seizures were elicited by a subconvulsant dose of pilocarpine (100 mg/kg) only after NOS inhibition. NOS inhibition also simultaneously potentiated the severity of PTZ-induced limbic seizures (60 mg/kg) and protected against PTZ-induced tonic seizures (80 mg/kg). The audiogenic seizure susceptibility of S or R rats did not change after similar treatments. In conclusion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental epilepsy; Seizures; Forebrain; Brainstem; Pilocarpine; Pentylenetetrazol; Audiogenic seizures; Genetically epilepsy-prone rats; GEPR; Nitric oxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800010

Assessment of the progressive nature of cell damage in the pilocarpine model of epilepsy
Provedor de dados: 56
Autores: Covolan,L.; Mello,L.E.
Pilocarpine-induced (320 mg/kg, ip) status epilepticus (SE) in adult (2-3 months) male Wistar rats results in extensive neuronal damage in limbic structures. Here we investigated whether the induction of a second SE (N = 6) would generate damage and cell loss similar to that seen after a first SE (N = 9). Counts of silver-stained (indicative of cell damage) cells, using the Gallyas argyrophil III method, revealed a markedly lower neuronal injury in animals submitted to re-induction of SE compared to rats exposed to a single episode of pilocarpine-induced SE. This effect could be explained as follows: 1) the first SE removes the vulnerable cells, leaving behind resistant cells that are not affected by the second SE; 2) the first SE confers increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell injury; Status epilepticus; Epilepsy; Pilocarpine; Spontaneous seizures.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700010

Effect of pilocarpine mouthwash on salivary flow
Provedor de dados: 56
Autores: Bernardi,R.; Perin,C.; Becker,F.L.; Ramos,G.Z.; Gheno,G.Z.; Lopes,L.R.; Pires,M.; Barros,H.M.T.
Pilocarpine is a cholinergic agonist that increases salivary flow and has been used to treat xerostomia. Oral intake is the most frequent route of administration. Adverse effects are dose-dependent and include sudoresis, facial blushing and increased urinary frequency. The objective of the present study was to evaluate the effects of topical pilocarpine solutions as mouthwashes on salivary flow and their adverse effects on healthy subjects. Forty volunteers received 10 ml 0.5, 1 and 2% pilocarpine solutions or 0.9% saline in a randomized, double-blind, placebo-controlled manner. Salivation was measured before and 45, 60 and 75 min after mouth rinsing for 1 min with 10 ml of saline or pilocarpine solutions. Vital signs were measured and ocular,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pilocarpine; Mouth rinsing; Mouthwash; Xerostomia; Salivation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100015

Comparison of sweat rate during graded exercise and the local rate induced by pilocarpine
Provedor de dados: 56
Autores: Vimieiro-Gomes,A.C.; Magalhães,F.C.; Amorim,F.T.; Machado-Moreira,C.A.; Rosa,M.S.; Lima,N.R.V.; Rodrigues,L.O.C.
Centrally stimulated sweat rate produced by graded exercise until exhaustion was compared to the local sweat rate induced by pilocarpine, often used as a sweating index for healthy individuals. Nine young male volunteers (22 ± 4 years) were studied in temperate environment in two situations: at rest and during progressive exercise with 25 W increases every 2 min until exhaustion, on a cycle ergometer. In both situations, sweating was induced on the right forearm with 5 ml 0.5% pilocarpine hydrochloride applied by iontophoresis (1.5 mA, 5 min), with left forearm used as control. Local sweat rate was measured for 15 min at rest. During exercise, whole-body sweat rate was calculated from the body weight variation. Local sweat rate was measured from the time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sweat rate; Graded exercise test; Pilocarpine; Thermoregulation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700017

Role of nitric oxide of the median preoptic nucleus (MnPO) in the alterations of salivary flow, arterial pressure and heart rate induced by injection of pilocarpine into the MnPO and intraperitoneally
Provedor de dados: 56
Autores: Saad,Wilson A.; Guarda,I.F.M.S.; Camargo,L.A.A.; Santos,T.A.F.B.; Guarda,R.S.; Saad,Willian A.; Simões,S.; Antunes Rodrigues,J.
We investigated the effect of L-NAME, a nitric oxide (NO) inhibitor and sodium nitroprusside (SNP), an NO-donating agent, on pilocarpine-induced alterations in salivary flow, mean arterial blood pressure (MAP) and heart rate (HR) in rats. Male Holtzman rats (250-300 g) were implanted with a stainless steel cannula directly into the median preoptic nucleus (MnPO). Pilocarpine (10, 20, 40, 80, 160 µg) injected into the MnPO induced an increase in salivary secretion (P&lt;0.01). Pilocarpine (1, 2, 4, 8, 16 mg/kg) ip also increased salivary secretion (P&lt;0.01). Injection of L-NAME (40 µg) into the MnPO prior to pilocarpine (10, 20, 40, 80, 160 µg) injected into the MnPO or ip (1, 2, 4, 8, 16 mg/kg) increased salivary secretion (P&lt;0.01). SNP (30 µg)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pilocarpine; Nitric oxide; Central nervous system; Salivation; Arterial pressure; Heart rate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700010