Consulta: subjectFacets:"Polymorphism"
Registros recuperados: 189
Data/hora: 03/06/2024 04:32:27

Análisis de la diversidad genética de variedades nativas de chile "Poblano" por medio de microsatélites.
Provedor de dados: 32
Autores: Contreras Toledo, Aremi Rebeca.
A partir de la domesticación de chile (Capsicum annuum L.) en México, se ha generado una gran diversidad dentro de esta especie en nuestro país, derivada de la selección de los agricultores para diferentes usos, siendo el chile Poblano uno de los más importantes y el más representativo del Estado de Puebla; sin embargo, el rendimiento de chile poblano en esta región ha presentado una reducción en los últimos años, debido, en parte, a la falta de semilla mejorada que debe ser seleccionada con base en las necesidades de los propios productores, existiendo la potencial pérdida de este germoplasma. Los objetivos de este estudio fueron estimar la diversidad genética y determinar sus posibles patrones de distribución, diferenciar grupos genéticos de variedades y...
Palavras-chave: Capsicum annuum; SSR; Polimorfismo; Diferenciación genética; Polymorphism; Genetic differentiation; Maestría; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/400

Diversidad genética y patrón de cruzamiento en poblaciones naturales de Pseudotsuga menziesii (Mirb.) Franco
Provedor de dados: 32
Autores: Cruz Nicolas, Jorge.
Se determinó la estructura de la diversidad genética y el patrón de cruzamiento en poblaciones naturales de Pseudotsuga menziesii (Mirb.) Franco en México con base en información de isoenzimas. Para evaluar la diversidad genética se utilizó una muestra de 11 poblaciones naturales distribuidas en diferentes regiones geográficas (noroeste, noreste y centro) del país; el patrón de cruzamiento y su efecto sobre el nivel de endogamia sólo se evaluó en una muestra de tres poblaciones, representativas de cada región geográfica. Se encontró una amplia diversidad genética a nivel de especie (83.3% de loci polimórficos y un promedio de 2.9 alelos por locus), pero reducida a nivel de poblaciones (28.3 % de loci polimórficos y 1.52 alelos por locus), especialmente en...
Tipo: Tesis
Palavras-chave: Abeto Douglas; Distancias genéticas; Diversidad genética; Endogamia; Isoenzimas; Polimorfismo; Polinización cruzada; Maestría; Genética; Cross-pollination; Douglas-fir; Genetic distances; Genetic diversity; Inbreeding; Isozymes; Polymorphism.
Ano: 2007
URL: http://hdl.handle.net/10521/1587

Factor 9 de crecimiento y diferenciación asociado al índice de prolificidad en la oveja pelibuey.
Provedor de dados: 32
Autores: Pérez Ruíz, Elizabeth.
El Factor 9 de Crecimiento y Diferenciación (GDF9) es miembro de la súperfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes mutaciones en el gen GDF9 han sido asociadas con el incremento de tasa de ovulación y/o prolificidad en algunas razas de ovejas, por lo que el objetivo de este estudio fue la búsqueda de polimorfismos de una sola base (SNPs) en el gen GDF9, así como la asociación entre polimorfismos del gen GDF9 y el índice de prolificidad en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 16 ovejas y fueron conservadas en papel FTA® (Whatman Mini Card). El exón dos del gen fue amplificado mediante la técnica de Polimerase Chain...
Palavras-chave: Análisis de secuencias; GDF9; Mutación; Polimorfismo; PCR; SNP; Sequence analysis; Mutation; Polymorphism; Maestría; Ganadería.
Ano: 2012
URL: http://hdl.handle.net/10521/1712

Poliembrionía e identificación de embriones cigóticos y nucelares de mango (Mangifera indica L.) cvs. manila y ataulfo.
Provedor de dados: 32
Autores: Martínez Ochoa, Elisa del Carmen.
Identificar el origen cigótico o nucelar de los embriones en semillas poliembriónicas de mango (Mangifera indica L.) facilitará la selección de híbridos y el mejoramiento genético, la reproducción clonal de portainjertos libres de enfermedades, y la renovación de clones viejos que han perdido el vigor híbrido. El objetivo de esta investigación fue estudiar las características de fruto, semilla y embriones en los cvs. Manila y Ataulfo y su relación con poliembrionía, e identificar plántulas cigóticas y nucelares originadas de embriones cultivados in vitro mediante marcadores RAPD. En cada cultivar fueron evaluadas las características morfológicas de 40 frutos, semillas y sus embriones. En ‘Manila’ y ‘Ataulfo’ se encontró correlación positiva entre el peso...
Palavras-chave: Mangifera indica L.; Embriones sexuales; Germinación in vitro; Marcadores moleculares; Polimorfismo; Sexual embryos; Sprouting in vitro; Molecura markers; Polymorphism; Maestría; Fisiología Vegetal.
Ano: 2010
URL: http://hdl.handle.net/10521/175

Análisis de la diversidad genética de variedades nativas de chile "Poblano" por medio de microsatélites.
Provedor de dados: 32
Autores: Contreras Toledo, Aremi Rebeca.
A partir de la domesticación de chile (Capsicum annuum L.) en México, se ha generado una gran diversidad dentro de esta especie en nuestro país, derivada de la selección de los agricultores para diferentes usos, siendo el chile Poblano uno de los más importantes y el más representativo del Estado de Puebla; sin embargo, el rendimiento de chile poblano en esta región ha presentado una reducción en los últimos años, debido, en parte, a la falta de semilla mejorada que debe ser seleccionada con base en las necesidades de los propios productores, existiendo la potencial pérdida de este germoplasma. Los objetivos de este estudio fueron estimar la diversidad genética y determinar sus posibles patrones de distribución, diferenciar grupos genéticos de variedades y...
Palavras-chave: Capsicum annuum; SSR; Polimorfismo; Diferenciación genética; Polymorphism; Genetic differentiation; Maestría; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/400

Poliembrionía e identificación de embriones cigóticos y nucelares de mango (Mangifera indica L.) cvs. manila y ataulfo.
Provedor de dados: 32
Autores: Martínez Ochoa, Elisa del Carmen.
Identificar el origen cigótico o nucelar de los embriones en semillas poliembriónicas de mango (Mangifera indica L.) facilitará la selección de híbridos y el mejoramiento genético, la reproducción clonal de portainjertos libres de enfermedades, y la renovación de clones viejos que han perdido el vigor híbrido. El objetivo de esta investigación fue estudiar las características de fruto, semilla y embriones en los cvs. Manila y Ataulfo y su relación con poliembrionía, e identificar plántulas cigóticas y nucelares originadas de embriones cultivados in vitro mediante marcadores RAPD. En cada cultivar fueron evaluadas las características morfológicas de 40 frutos, semillas y sus embriones. En ‘Manila’ y ‘Ataulfo’ se encontró correlación positiva entre el peso...
Palavras-chave: Mangifera indica L.; Embriones sexuales; Germinación in vitro; Marcadores moleculares; Polimorfismo; Sexual embryos; Sprouting in vitro; Molecura markers; Polymorphism; Maestría; Fisiología Vegetal.
Ano: 2010
URL: http://hdl.handle.net/10521/175

Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
Provedor de dados: 69
Autores: Yuan,Mei; Gong,Limin; Meng,Ronghua; Li,Shuangling; Dang,Phat; Guo,Baozhu; He,Guohao.
Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was...
Tipo: Journal article
Palavras-chave: Cultivated peanut; Microsatellites; Polymorphism; Simple sequence repeat.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005

EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)
Provedor de dados: 69
Autores: Song,Guo Qi; Li,Meng Jun; Xiao,Han; Wang,Xing Jun; Tang,Rong Hua; Xia,Han; Zhao,Chuan Zhi; Bi,Yu Ping.
Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA...
Tipo: Journal article
Palavras-chave: Arachis hypogaea L.; Cultivars; EST-SSR; Polymorphism; Wild-type peanut.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007

Association between AA-NAT gene polymorphism and reproductive performance in sheep
Provedor de dados: 69
Autores: Ding-ping,Bai; Cheng-jiang,Yu; Yu-lin,Chen.
Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A &amp;gt; G) was &amp;#64257;rstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A &amp;gt; G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus...
Tipo: Journal article
Palavras-chave: AA-NAT gene; Polymorphism; Reproduction; Seasonality; Sheep.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200007

PCR-RFLP as a useful tool for diagnosis of invasive mycoses in a healthcare facility in the North of Brazil
Provedor de dados: 69
Autores: Sousa,Diego Rayan Teixeira de; Santos,Carla Silvana da Silva; Wanke,Bodo; Silva Júnior,Roberto Moreira da; Santos,Marcelo Cordeiro dos; Cruz,Kátia Santana; Monte,Rossicléia Lins; Nocker,Andreas; Souza,João Vicente Braga de.
Background The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses. In addition, the new approach was applied to detect fungal agents in 120 biological samples (blood, cerebrospinal fluid and bone marrow). PCR-RFLP results were compared with the ones obtained with conventional methods (culture, microscopy, and biochemical testing). Results The assays carried out with the reference strains (Candida albicans, Candida parapsilosis, Candida tropicalis, Candida krusei, Candida guilliermondii, Cryptococcus neoformans, Cryptococcus gattii and Histoplasma capsulatum), demonstrated that the RFLP profiles were correctly predicted by the in silico...
Tipo: Journal article
Palavras-chave: Pathogenic fungus; Polymorphism; Simultaneous analysis.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300014

Polymorphism of four microsatellites and their polymerisation effect on litter size in Boer goats
Provedor de dados: 69
Autores: Wang,Jian gang; Hou,Jin-Xing; Li,Guang; Zhu,Guang Qin; Cao,BinYun.
Background: Finding molecular markers linked to quantitative trait loci is the first step in marker-assisted selection (MAS). Microsatellites are excellent molecular markers because of their large numbers, even distribution in the genome, and high polymorphism. In this study, the polymerisation effect of four microsatellites (OarAE101, BM1329, BM143, and LSCV043) on litter size was analysed using microsatellite markers and pedigrees. Results: The results indicate that the polymerisation effect of four microsatellite loci significantly affected the litter size. E5E10F2F6G1G5H6H11 and E3E8F5F7G1G5H3H9 had the highest and lowest litter sizes in the F2 generation, respectively. The polymerisation effect value (v) of the E5E10 genotype was 3.18% higher than...
Tipo: Journal article
Palavras-chave: Microsatellite marker; Pedigrees; Polymerisation effect; Polymorphism.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400011

Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE
Provedor de dados: 69
Autores: Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article
Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001

Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars
Provedor de dados: 69
Autores: Al-Faifi,Sulieman A; Migdadi,Hussein M; Algamdi,Salem S; Altaf Khan,Mohammad; Ammar,Megahed H; Al-Obeed,Rashid S; I. Al-Thamra,Mohammad; EL-Harty,Ehab H; Jakse,Jerenj.
Background: The present study was undertaken towards the development of SSR markers and assessing genetic relationships among 32 date palm (Phoenix dactylifera L.) representing common cultivars grown in different geographical regions in Saudi Arabia. Results: Ninety-three novel simple sequence repeat markers were developed and screened for their ability to detect polymorphism in date palm. Around 71% of genomic SSRs were dinucleotide, 25% tri, 3% tetra and 1% penta nucleotide motives. Twenty-two primers generated a total of 91 alleles with a mean of 4.14 alleles per locus and 100% polymorphism percentage. A 0.595 average polymorphic information content and 0.662 primer discrimination power values were recorded. The expected and observed heterozygosities...
Tipo: Journal article
Palavras-chave: Expected and observed heterozygosity; Microsatellite markers; Polymorphic information content; Polymorphism; Primer discrimination power.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300002

Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos
Provedor de dados: 69
Autores: Li,Ai Qin; Zhao,Chuan Zhi; Wang,Xing Jun; Liu,Zhan Ji; Zhang,Li Feng; Song,Guo Qi; Yin,Juan; Li,Chang Sheng; Xia,Han; Bi,Yu Ping.
Microsatellites, or simple sequence repeats (SSRs), in expressed sequence tags (ESTs) provide an opportunity for low cost SSR development. We looked for EST-SSRs in 403,511 ESTs (generated by 454 sequencing and representing 70,654 contigs and 52,082 singletons) from soybean globular stage embryos. Among 122,736 unique ESTs, 3,729 contained one or more SSRs. In total, 3,989 SSRs were identified including 304 mono, 1,374 di, 2,208 tri, 70 tetra, 13 penta and 20 hexanucleotide SSRs. Thirty three EST-SSRs were selected for primer design and polymorphism analysis using twenty soybean cultivars and one wild-type soybean. Successful amplification was obtained using 21 of these primer pairs, 11 of which detected polymorphisms in these soybean cultivars. These...
Tipo: Journal article
Palavras-chave: 454 sequencing; EST; Glycine max; Polymorphism; SSR.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500006

Combined genotypes of the MBL2 gene related to low mannose-binding lectin levels are associated with vaso-occlusive events in children with sickle cell anemia
Provedor de dados: 74
Autores: Medeiros,Fernanda Silva; Mendonça,Taciana Furtado de; Lopes,Katiuscia Araújo de Miranda; França,Laís Medeiros da Câmara; Silva,Andreia Soares da; Vasconcelos,Luydson Richardson Silva; Oliveira,Maria do Carmo Valgueiro Costa de; Anjos,Ana Cláudia Mendonça dos; Hatzlhofer,Betânia Lucena Domingues; Bezerra,Marcos André Cavalcanti; Araújo,Aderson da Silva; Moura,Patrícia; Cavalcanti,Maria do Socorro de Mendonça.
Abstract Sickle cell anemia (SCA) presents heterogenous clinical manifestations that cannot be explained solely by alterations to hemoglobin (Hb); other components such as endothelial adhesion, thrombosis and inflammation may be involved. The mannose-binding lectin (MBL) has an important role in innate immunity and inflammatory diseases. In this report, we describe an association between MBL2 polymorphism related to low production of serum MBL and the frequency of vasoocclusive events (FVOE) in children ≤ 5 years old with SCA (p = 0.0229; OR 5.55; CI 1.11-27.66). Further studies are needed to explore the role of low MBL2 in the pathophysiology of vasoocclusive events in SCA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MBL2; Polymorphism; Sickle cell anemia; Vaso-occlusive events.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400600

Polymorphism of locus DRB3.2 in populations   of Creole Cattle from Northern Mexico
Provedor de dados: 74
Autores: Fernández,Ilda G.; Ramírez,José Gonzalo Ríos; Vázquez,Amanda Gayosso; Arvizu,Raúl Ulloa; Morales,Rogelio A. Alonso.
The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916,...
Palavras-chave: BoLA-DRB3.2; Polymorphism; Creole cattle; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013

The cuttable C-related genotype and allele for the E-cadherin 3’-UTR Pml I polymorphism are associated with higher susceptibility to endometriosis
Provedor de dados: 74
Autores: Hsieh,Yao-Yuan; Chang,Chi-Chen; Tsai,Fuu-Jen; Hsu,Chin-Moo; Lin,Cheng-Chieh; Tsai,Chang-Hai.
Epithelial cadherin (E-cadherin; CDH1) may influence pericellular proteolysis and intracellular signal transduction, which plays an essential part of tumor invasion. In our study we investigated the correlation between CDH1 gene polymorphism and endometriosis in two groups of pre-menopausal Taiwanese women, group 1 (n = 150) consisting of women with severe stage IV endometriosis and group 2 (n = 159) of women with no endometriosis. The polymerase chain reaction (PCR) was used to identify the cuttable (C) and uncuttable (T) polymorphism of the CDH1-Pml I gene (rs1801026) located on the 3’-untranslated region (3’-UTR) of chromosome 16 and compare the genotypes and allelic frequencies of this gene in both groups. We found that the genotype and allele...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Endometriosis; E-cadherin; Polymorphism; SNP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500003

Aromatase gene and its effects on growth, reproductive and maternal ability traits in a multibreed sheep population from Brazil
Provedor de dados: 74
Autores: Lôbo,Ana Maria Bezerra Oliveira; Lôbo,Raimundo Nonato Braga; Paiva,Samuel Rezende.
We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals were genotyped using the PCR-RFLP technique. The influence of the animal's genotype on its performance or on the performance of its lambs was analyzed by the least square method. Another factor assessed was the importance of the animal's genotype in analysis models for quantitative breeding value estimates, and whether there were differences among the averages of breeding values of animals with different genotypes for this gene. In the sample...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body weight; Lambing interval; PCR-RFLP; Polymorphism; SNP; Litter weight.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300010

A novel point mutation in a class IV glucose-6-phosphate dehydrogenase variant (G6PD São Paulo) and polymorphic G6PD variants in São Paulo State, Brazil
Provedor de dados: 74
Autores: Oliveira,Raimundo Antonio G.; Oshiro,Marilena; Hirata,Mario H.; Hirata,Rosario D.C.; Ribeiro,Georgina S.; Medeiros,Tereza M.D.; Barretto,Orlando C. de O.
In this study, we used red cell glucose-6-phosphate dehydrogenase (G6PD) activity to screen for G6PD-deficient individuals in 373 unrelated asymptomatic adult men who were working with insecticides (organophosphorus and carbamate) in dengue prevention programs in 27 cities in São Paulo State, Brazil. Twenty-one unrelated male children suspected of having erythroenzymopathy who were attended at hospitals in São Paulo city were also studied. Fifteen of the 373 adults and 12 of the 21 children were G6PD deficient. G6PD gene mutations were investigated in these G6PD-deficient individuals by using PCR-RFLP, PCR-SSCP analysis and DNA sequencing. Twelve G6PD A-202A/376G and two G6PD Seattle844C, as well as a new variant identified as G6PD São Paulo, were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose-6-phosphate dehydrogenase; Mutations; Polymorphism; Variants.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200007

Haptoglobin gene subtypes in three Brazilian population groups of different ethnicities
Provedor de dados: 74
Autores: Miranda-Vilela,Ana L.; Akimoto,Arthur K.; Alves,Penha C.Z.; Hiragi,Cássia O.; Penalva,Guilherme C.; Oliveira,Silviene F.; Grisolia,Cesar K.; Klautau-Guimarães,Maria N.
Haptoglobin is a plasma hemoglobin-binding protein that limits iron loss during normal erythrocyte turnover and hemolysis, thereby preventing oxidative damage mediated by iron excess in the circulation. Haptoglobin polymorphism in humans, characterized by the Hp*1 and Hp*2 alleles, results in distinct phenotypes known as Hp1-1, Hp2-1 and Hp2-2, whose frequencies vary according to the ethnic origin of the population. The Hp*1 allele has two subtypes, Hp*1F and Hp*1S, that also vary in their frequencies among populations worldwide. In this work, we examined the distribution frequencies of haptoglobin subtypes in three Brazilian population groups of different ethnicities. The haptoglobin genotypes of Kayabi Amerindians (n = 56), Kalunga Afro-descendants (n =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian ethnicities; Haptoglobin; Polymorphism; Subtypes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300005

Biochemical comparison of two Hypostomus populations (Siluriformes, Loricariidae) from the Atlântico Stream of the upper Paraná River basin, Brazil
Provedor de dados: 74
Autores: Ito,Kennya F.; Renesto,Erasmo; Zawadzki,Cláudio H.
Two syntopic morphotypes of the genus Hypostomus - H. nigromaculatus and H. cf. nigromaculatus (Atlântico Stream, Paraná State) - were compared through the allozyme electrophoresis technique. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, attributing the score of 20 loci, with a total of 30 alleles. Six loci were diagnostic (Aat-2, Gcdh-1, Gpi-A, Idh-1, Ldh-A and Mdh-A), indicating the presence of interjacent reproductive isolation. The occurrence of few polymorphic loci acknowledge two morphotypes, with heterozygosity values He = 0.0291 for H. nigromaculatus and He = 0.0346 for H. cf. nigromaculatus. F IS statistics demonstrated fixation of the alleles in the two morphotypes. Genetic identity (I)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Hypostomus nigromaculatus; Fish genetics; Genetic distance; Polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100008

Comparative genome-wide polymorphic microsatellite markers in Antarctic penguins through next generation sequencing
Provedor de dados: 74
Autores: Vianna,Juliana A.; Noll,Daly; Mura-Jornet,Isidora; Valenzuela-Guerra,Paulina; González-Acuña,Daniel; Navarro,Cristell; Loyola,David E.; Dantas,Gisele P. M.
Abstract Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics. We characterized a large set of genome-wide microsatellites and evaluated polymorphisms in all three species. SOLiD reads were generated from the libraries of each species, identifying a large amount of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Penguin; Genome; Polymorphism; Tetranucleotide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400676

CTLA-4 gene polymorphisms are associated with obesity in Turner Syndrome
Provedor de dados: 74
Autores: Santos,Luana Oliveira dos; Bispo,Adriana Valéria Sales; Barros,Juliana Vieira de; Laranjeira,Raysa Samanta Moraes; Pinto,Rafaella do Nascimento; Silva,Jaqueline de Azevêdo; Duarte,Andréa de Rezende; Araújo,Jacqueline; Sandrin-Garcia,Paula; Crovella,Sergio; Bezerra,Marcos André Cavalcanti; Belmont,Taciana Furtado de Mendonça; Cavalcanti,Maria do Socorro; Santos,Neide.
Abstract Turner syndrome (TS) is characterized by a set of clinical conditions, including autoimmune/inflammatory diseases and infectious conditions, that can compromise a patient’s quality of life. Here we assessed polymorphisms in CTLA-4 +49A/G (rs231775), PTPN22 +1858G/A (rs2476601), and MBL2 -550 (H/L) (rs11003125), -221(X/Y) (rs7096206) and exon 1 (A/O) in women from northeastern Brazil to determine whether polymorphisms within these key immune response genes confer differential susceptibility to clinical conditions in TS. A case-control genetic association study was performed, including 86 female TS patients and 179 healthy women. An association was observed for the A/G genotype of CTLA-4 +49A/G in TS patients (p=0.043, odds ratio [OR]=0.54). In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTLA-4 gene; Immune genes; Obesity; Polymorphism; Turner syndrome.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500727

Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
Provedor de dados: 74
Autores: Novelli,Valdenice M.; Cristofani,Mariangela; Souza,Alessandra A.; Machado,Marcos A.
The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences from which 171 primer pairs were designed of which 113 with the best banding patterns were selected. Characterization of sweet orange microsatellite loci revealed that AG/TC was the most abundant (69%) microsatellite class isolated, followed by GT/CA (15.9%), TCA/AGT (8%) and AAC/TTG (6.2%). The number of alleles ranged from 1 to 4, with a mean of 2 alleles per locus. Four microsatellite loci developed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus species; Germplasm bank; Polymorphism; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100018

Association of XRCC1 Arg399GIn and Tp53 Arg72Pro polymorphisms and increased risk of uterine leiomyoma - A case-control study
Provedor de dados: 74
Autores: Yaghmaei,Minoo; Salimi,Saeedeh; Namazi,Lida; Farajian-Mashhadi,Farzaneh.
Abstract The aim of present study was to investigate the role of the X-ray repair cross-complementing protein1 (XRCC1) and Tumor protein p53 (Tp53) polymorphisms in Uterine Leiomyoma (UL) susceptibility in southeastern Iran. This case control study was performed on 139 women with UL and 149 age, BMI and ethnicity matched healthy women. All women were genotyped for the XRCC1 Arg399Gln, XRCC1 Arg194Trp and Tp53 Arg72Pro polymorphisms. The frequency of Tp53 72 Pro/Pro genotype was significantly higher in UL women compared to controls. The risk of UL was 1.5 fold higher in women with the Pro/Pro genotype (OR, 1.5 [95% CI, 1.1 to 2.1], p = 0.012). Moreover, the frequency of the Pro allele was significantly higher in the UL women. Although the frequency of XRCC1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine leiomyoma; Tp53; XRCC1; Polymorphism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400444

Genetic variability of 10 microsatellite markers in the characterization of Brazilian Nellore cattle (Bos indicus)
Provedor de dados: 74
Autores: Cervini,Marcelo; Henrique-Silva,Flávio; Mortari,Norma; Matheucci Jr,Euclides.
We assessed the polymorphism of 10 microsatellites in Brazilian Nellore cattle (Bos indicus) using a commercial multiplex system. Allele frequencies, polymorphism information content, heterozygosity and exclusion probability were calculated. Allele frequencies revealed that in the sample analyzed the markers were not equally polymorphic. The exclusion probabilities and the polymorphism information content of some loci in Nellore cattle were lower than in Bos taurus breeds. When all the microsatellites were considered the combined exclusion probability was 0.9989. This multiplex analysis can contribute toward pedigree information, adequate genetic improvements and breeding programs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alleles; Frequencies; Microsatellite; Nellore; Polymorphism; Zebu.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300015

Three novel α-L-iduronidase mutations in 10 unrelated Chinese mucopolysaccharidosis type I families
Provedor de dados: 74
Autores: Sun,Luning; Li,Chunyi; Song,Xiaoyu; Zheng,Ningning; Zhang,Haipeng; Dong,Guizhang.
Mucopolysaccharidosis type I (MPS I) arises from a deficiency in the α-L-iduronidase (IDUA) enzyme. Although the clinical spectrum in MPS I patients is continuous, it was possible to recognize 3 phenotypes reflecting the severity of symptoms, viz., the Hurler, Scheie and Hurler/Scheie syndromes. In this study, 10 unrelated Chinese MPS I families (nine Hurler and one Hurler/Scheie) were investigated, and 16 mutant alleles were identified. Three novel mutations in IDUA genes, one missense p.R363H (c.1088G &gt; A) and two splice-site mutations (c.1190-1G &gt; A and c.792+1G &gt; T), were found. Notably, 45% (nine out of 20) and 30% (six out of 20) of the mutant alleles in the 10 families studied were c.1190-1G &gt; A and c.792+1G &gt; T, respectively. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolysaccharidosis type I; Α-L-iduronidase; Mutation; Polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200004

The MTHFR C677T polymorphism and global DNA methylation in oral epithelial cells
Provedor de dados: 74
Autores: Arruda,Isabela Tatiana Sales de; Persuhn,Darlene Camati; Oliveira,Naila Francis Paulo de.
DNA methylation is mediated by DNA methyltransferases (DNMTs) that add a methyl group to the 5'-carbon of cytosine. The enzyme methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the rate-limiting step of the cycle involving the methyl donor S-adenosyl-L-methionine (SAM). The MTHFR C677T polymorphism results in a thermolabile enzyme with reduced activity that is predicted to influence the DNA methylation status. In this study, we investigated the impact of the MTHFR C677T polymorphism on the global DNA methylation of oral epithelial cells obtained from 54 healthy subjects. There were no significant differences in global DNA methylation among the MTHFR CC, CT and TT genotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Epigenetic; MTHFR C677T; Oral epithelial cells; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400004

Characterization of 13 microsatellite loci developed from Meconopsis horridula
Provedor de dados: 74
Autores: Zhao,Yu; Zhang,Shi-Bao; Yang,Jing; Zhang,Lu.
Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic structure; Heterozygosity; Meconopsis horridula; Microsatellite markers; Polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026

Analysis of genetic diversity and population structure in Argentine and Bolivian Creole cattle using five loci related to milk production
Provedor de dados: 74
Autores: Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G.
Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010

Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production potential of beef cattle using the Brazilian Canchim breed as a model
Provedor de dados: 74
Autores: Silveira,Luiz Guilherme G.; Furlan,Luiz R.; Curi,Rogério A.; Ferraz,André Luiz J.; Alencar,Maurício M. de; Regitano,Luciana C.A.; Martins,Cyntia L.; Arrigoni,Mário de Beni; Suguisawa,Liliane; Silveira,Antônio C.; Oliveira,Henrique N. de.
The growth hormone 1 gene (GH1) is a candidate gene for body weight and weight gain in cattle since it plays a fundamental role in growth regulation. We investigated the GH1 gene AluI and DdeI restriction enzyme polymorphisms, located 149 bp apart in the cattle genome, as possible markers of the production potential of Canchim crossbreed cattle, a 5/8 Charolais (Bos taurus) and 3/8 Nelore (Bos indicus) breed developed in Brazil, by evaluating the birth weight, weaning weight, yearling weight and plasma insulin-like growth factor-1 (IGF-1) concentration of 7 month to 10 months old Canchim calves (n = 204) of known genealogy and which had been genotyped for the AluI and DdeI markers. Our results showed significant effect (p &lt; 0.05) between the homozygous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Polymorphism; Growth hormone; Candidate gene.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500012

HLA-DRB1 alleles in four Amerindian populations from Argentina and Paraguay
Provedor de dados: 74
Autores: Parolín,Maria L.; Carnese,Francisco R.
The major histocompatibility complex (MHC) is one of the biological systems of major polymorphisms. The study of HLA class II variability has allowed the identification of several alleles that are characteristic to Amerindian populations, and it is an excellent tool to define the relations and biological affinities among them. In this work, we analyzed the allelic distribution of the HLA-DRB1 class II locus in four Amerindian populations: Mapuche (n = 34) and Tehuelche (n = 23) from the Patagonian region of Argentina, and Wichi SV (n = 24) and Lengua (n = 17) from the Argentinean and Paraguayan Chaco regions, respectively. In all of these groups, relatively high frequencies of Amerindian HLA-DRB1 alleles were observed (DRB1*0403, DRB1*0407, DRB1*0411,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-DRB1; Polymorphism; Amerindians; Biological affinities.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200002

Association of T174M polymorphism of angiotensinogen gene with essential hypertension: a meta-analysis
Provedor de dados: 74
Autores: Liao,Xiaoyang; Yang,Zhiyi; Peng,Daqing; Dai,Hua; Lei,Yi; Zhao,Qian; Han,Yanbing; Wang,Weiwen.
The association between T174M polymorphism of angiotensinogen gene and essential hypertension risk remains controversial. We herein performed a meta-analysis to achieve a reliable estimation of their relationship. All the studies published up to May 2013 on the association between T174M polymorphism and essential hypertension risk were identified by searching the electronic repositories PubMed, MEDLINE and EMBASE, Springer, Elsevier Science Direct, Cochrane Library and Google Scholar. Data were extracted and pooled odds ratios (ORs) with 95% confidence intervals (95% CIs) were calculated. Ultimately, nine eligible studies, including 2188 essential hypertension cases and 2459 controls, were enrolled in this meta-analysis. No significant associations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential hypertension; Case-control study; T174M; Polymorphism; Meta-analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400001

Reproductive strategies and genetic variability in tropical freshwater fish
Provedor de dados: 74
Autores: Lassala,Maria Dolores Peres; Renesto,Erasmo.
We estimated the genetic variability of nine fish species from the Brazilian upper Paraná River floodplain (Astyanax altiparanae, Hoplias malabaricus, Leporinus lacustris, Loricariichthys platymetopon, Parauchenipterus galeatus, Pimelodus maculatus, Rhaphiodon vulpinus, Roeboides paranensis and Serrasalmus marginatus) based on data for 36 putative allozyme loci obtained using corn starch gel electrophoresis of 13 enzymatic systems: aspartate aminotransferase (EC 2.6.1.1), acid phosphatase (EC 3.1.3.2), esterase (EC 3.1.1.1), glycerol-3-phosphate dehydrogenase (EC 1.1.1.8), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), glucose-6-phosphate isomerase (EC 5.3.1.9), Iditol dehydrogenase (EC 1.1.1.14), isocitrate dehydrogenase - NADP+ (EC 1.1.1.42), L-lactate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Heterozygosity; Paraná River; Polymorphism; Reproductive strategies; Tropical fish.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400030

Robertsonian rearrangements in the reef fish Chromis (Perciformes, Pomacentridae) involving chromosomes bearing 5s rRNA genes
Provedor de dados: 74
Autores: Molina,Wagner F.; Galetti-Jr,Pedro M.
Cytogenetic studies were done on three Pomacentridae species of the genus Chromis. The karyotype of C. multilineata consisted of 48 acrocentric chromosomes (FN = 48), C. insolata had 2n = 46-47 (3-4M+6SM+36-38A; FN = 56) and C. flavicauda had 2n = 39 (9M+6SM+24A; FN = 54). Robertsonian polymorphisms were detected in C. insolata and C. flavicauda. All three species had small heterochromatic blocks restricted to centromeric regions. Nucleolar organizer regions (NORs) were detected in the telomeric position of a medium acrocentric chromosome pair in C. multilineata and in non-homologous chromosomes in both C. flavicauda and C. insolata. FISH with a telomeric probe detected no internal telomeric sequences in C. flavicauda and C. insolata. 5S rRNA genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromis; FISH; Polymorphism; Robertsonian rearrangements; 5S rDNA; Telomeric sequences.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400004

Associations of MYF5 gene polymorphisms with meat quality traits in different domestic pig (Sus scrofa) populations
Provedor de dados: 74
Autores: Liu,Min; Peng,Jian; Xu,Dequan; Zheng,Rong; Li,Feng’e; Li,Jialian; Zuo,Bo; Lei,Minggang; Xiong,Yuanzhu; Deng,Changyan; Jiang,Siwen.
The MYF5 gene is first inducibly expressed in muscle cell during embryonic muscle development and plays an important role in regulating the differentiation of skeletal muscle precursors. In this study we used PCR-RFLP to investigate two pig (Sus scrofa) populations (n = 302) for two MYF5 gene polymorphisms, a previously unreported novel Met-Leu shift single nucleotide polymorphism (SNP) MYF5/Hsp92II located on exon 1 and the previously identified intron 1 MYF5/HinfI SNP. Haplotype and association analysis showed that haplotypes of the two SNPs were significantly associated with drip loss rate (DLR, p &lt; 0.05), water holding capacity (WHC, p &lt; 0.05), biceps femoris meat color value (MCV2, p &lt; 0.05), biceps femoris marbling score (MM2, p &lt; 0.01),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meat quality; MYF5 gene; Pigs; Polymorphism; Sus scrofa.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300012

Characterization analysis and polymorphism detection of the porcine Myd88 gene
Provedor de dados: 74
Autores: Li,Xinyun; Liu,Huazhen; Shulin,Yang; Tang,Zhonglin; Ma,Yuehui; Chu,Mingxing; Li,Kui.
The myeloid differentiation primary response protein 88 (Myd88) is an essential adaptor protein, which mediates in all Toll-like receptor (TLR) members signal transduction, except for TLR3. In this study, the 4464 bp genomic sequence of porcine Myd88 was first isolated, whereupon tissue distribution, chromosome mapping and single nucleotide polymorphism (SNP) were analyzed. Our results revealed that porcine Myd88 gene, which was located at chromosome 13 linked with marker S0288 (distance = 40 cR; LOD = 8.66), was widely expressed in all the examined tissues. There were 16 potential SNPs in the isolated genome fragment. SNP 797T/C in the first intron was studied, with no significant association being found between the genotype and immune traits in pigs (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myd88; TLR; Polymorphism; Pig; Chromosome mapping.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200015

Genetic polymorphisms and haplotypes of the organic cation transporter 1 gene (SLC22A1) in the Xhosa population of South Africa
Provedor de dados: 74
Autores: Jacobs,Clifford; Pearce,Brendon; Du Plessis,Mornè; Hoosain,Nisreen; Benjeddou,Mongi.
Human organic cation transporter 1 is primarily expressed in hepatocytes and mediates the electrogenic transport of various endogenous and exogenous compounds, including clinically important drugs. Genetic polymorphisms in the gene coding for human organic cation transporter 1, SLC22A1, are increasingly being recognized as a possible mechanism explaining the variable response to clinical drugs, which are substrates for this transporter. The genotypic and allelic distributions of 19 nonsynonymous and one intronic SLC22A1 single nucleotide polymorphisms were determined in 148 healthy Xhosa participants from South Africa, using a SNAPshot® multiplex assay. In addition, haplotype structure for SLC22A1 was inferred from the genotypic data. The minor allele...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Haplotype; Population genetic structure; Genotyping; Genetic variability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300006

Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
Provedor de dados: 74
Autores: Marino,Celso Luis; Gimenes,Marcos Aparecido; Silva,Norberto da; Lopes,Catalina Romero.
Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appeared to be very useful for hybrid identification, since the only condition required was polymorphism between the two parental lines. If the analyzed lines were used for hybrid production, 8.4% and 12.3% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively. If only one plant of each homozygous type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoenzyme; Polymorphism; Cauliflower.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100015

Association between osteoprotegerin gene polymorphisms and cardiovascular disease in type 2 diabetic patients
Provedor de dados: 74
Autores: Guo,Changlei; Hu,Fudong; Zhang,Shaoli; Wang,Yakun; Liu,Hengdao.
Osteoprotegerin (OPG) gene polymorphisms (T245G, T950C and G1181C) have been associated with osteoporosis and early predictors of cardiovascular disease. The aim of this study was to evaluate whether these polymorphisms contribute to cardiovascular disease (CVD) in type 2 diabetic patients. We performed a case-control study with 178 CVD subjects with diabetes and 312 diabetic patients without CVD to assess the impact of variants of the OPG gene on the risk of CVD. The OPG gene polymorphisms were analyzed by using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). There was no significant association between the T245G and G1181C polymorphisms and CVD in the additive genetic model (OR = 0.96, 95% CI 0.64-1.45, p = 0.79;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular disease; Osteoprotegerin; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200006

Valongo, genetic studies on an isolated Afro-Brazilian community
Provedor de dados: 74
Autores: Souza,Ilíada Rainha de; Culpi,Lodércio.
A southern Brazilian isolated community of predominantly sub-Saharan African origin, with a total population of 74 individuals and high degree of inbreeding (F = 0.081) was studied. The small sizes of the breeding (35) and effective (21) populations, as well as the very small effective migration rate (4%), suggest a high probability for the occurrence of genetic drift. A sample was typed for fourteen blood genetic systems and most of these systems seem to reveal the founder effect. This evolutionary factor was probably responsible for the absence of some polymorphic alleles frequent in African populations, i.e.: ABO*B, RHD-RHCE*DCe, GPA-GPB*NS (MNSs*NS), GPA-GPB*NS U (MNSs*NSU), HBB*S, HP*2M and ESD*2. The most unusual allele frequency was that for BCHE*A,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Isolated community; Polymorphism; Random genetic drift; Blood systems; Admixture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300012

Polymorphism of alpha-1-antitrypsin in hematological malignancies
Provedor de dados: 74
Autores: Topic,Aleksandra; Juranic,Zorica; Jelic,Svetislav; Magazinovic,Ivana Golubicic.
Alpha-1-antitrypsin (AAT) or serine protease inhibitor A1 (SERPINA1) is an important serine protease inhibitor in humans. The main physiological role of AAT is to inhibit neutrophil elastase (NE) released from triggered neutrophils, with an additional lesser role in the defense against damage inflicted by other serine proteases, such as cathepsin G and proteinase 3. Although there is a reported association between AAT polymorphism and different types of cancer, this association with hematological malignancies (HM) is, as yet, unknown. We identified AAT phenotypes by isoelectric focusing (in the pH 4.2-4.9 range) in 151 serum samples from patients with HM (Hodgkins lymphomas, non-Hodgkins lymphomas and malignant monoclonal gammopathies). Healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-1-antitrypsin; Polymorphism; Lymphomas.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400008

Polymorphisms in promoter sequences of MDM2, p53, and p16INK4a genes in normal Japanese individuals
Provedor de dados: 74
Autores: Ohsaka,Yasuhito; Nishino,Hoyoku.
Research has been conducted to identify sequence polymorphisms of gene promoter regions in patients and control subjects, including normal individuals, and to determine the influence of these polymorphisms on transcriptional regulation in cells that express wild-type or mutant p53. In this study we isolated genomic DNA from whole blood of healthy Japanese individuals and sequenced the promoter regions of the MDM2, p53, and p16INK4a genes. We identified polymorphisms comprising 3 nucleotide substitutions at exon 1 and intron 1 regions of the MDM2 gene and 1 nucleotide insertion at a poly(C) nucleotide position in the p53 gene. The Japanese individuals also exhibited p16INK4a polymorphisms at several positions, including position -191. Reporter gene analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Murine double minute 2; Polymorphism; P16INK4a; P53; Transcription.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400004

A SNP in 5′ untranslated region of CD40 gene is associated with an increased risk of ischemic stroke in a Chinese population: a case-control study
Provedor de dados: 74
Autores: Huang,Hua-Tuo; Guo,Jing; Xiang,Yang; Chen,Jian-Ming; Luo,Hong-Cheng; Meng,Lan-Qing; Wei,Ye-Sheng.
Abstract Cluster of differentiation 40 (CD40), the receptor for CD154, is a member of the tumor necrosis factor (TNF) receptor superfamily. Several studies have been conducted to investigate the effect of the CD40 rs1883832 polymorphism on atherosclerotic disease in different population; however, inconsistent results were obtained. In this study, we investigated the association of four polymorphisms (rs1883832, rs13040307, rs752118 and rs3765459) of CD40 gene and their effect on CD40 expression with the risk of ischemic stroke (IS) in a Chinese population. Three hundred and eighty patients with IS and 450 control subjects were included in the study. The CD40 polymorphisms were discriminated by Snapshot SNP genotyping assay. Serum soluble CD40 (sCD40)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD40; Gene; Polymorphism; Ischemic stroke.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300442

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP
Provedor de dados: 74
Autores: Raut,Ashwin A.; Kumar,Anil; Kala,Sheo N.; Chhokar,Vinod; Rana,Neeraj; Beniwal,Vikas; Jaglan,Sundeep; Samuchiwal,Sachin K.; Singh,Jitender K.; Mishra,Anamika.
Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo; DGAT1; PCR-SSCP; Polymorphism; SNP.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011

Contribution of DNA repair xeroderma pigmentosum group D genotypes to pancreatic cancer risk in the Chinese Han population
Provedor de dados: 74
Autores: Yan,Dong; Liang,Xiao-Hui; Ding,Wei; Xu,Xin-Jian; Wang,Xi-Yan.
Abstract This study aimed to determine the association between the polymorphisms and haplotypes in the xeroderma pigmentosum group D (XPD) gene and the risk of pancreatic cancer in the Chinese Han population. SNaPshot was used for genotyping six SNP sites of the XPD gene. Comparisons of the correlations between different genotypes in combination with smoking and the susceptibility to pancreatic cancer were performed. Individual pancreatic cancer risk in patients who carry mutant C alleles (AC, CC, and AC+CC) at rs13181 increased (p &lt; 0.05). Taking non-smoking individuals who carry the AA genotype as a reference, and non-smoking individuals who carry mutant allele C (AC+CC), the risk of pancreatic cancer increased by 3.343 times in individuals who smoked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic neoplasm; Human xeroderma pigmentosum group D; Polymorphism; Smoking.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100018

Adiponectin promoter polymorphisms are predictors of lipid profile improvement after bariatric surgery
Provedor de dados: 74
Autores: Gasparotto,Aline Simas; Borges,Diego Olschowsky; Zandoná,Marília Remuzzi; Ramos,Mauricio Jacques; Meihnardt,Nelson Guardiola; Mattevi,Vanessa S.
Abstract Our aim was to investigate if single nucleotide polymorphisms (SNPs) located in the 5′ regions of leptin (LEP, -2548 G &gt; A, rs7799039), resistin (RETN, -420 C &gt; G, rs1862513) and adiponectin (ADIPOQ, -11391 G &gt; A, rs17300539 and -11377 C &gt; G, rs266729) genes were related to changes in body mass index (BMI) and metabolic variables after bariatric surgery in 60 extremely obese individuals. At baseline, ADIPOQ -11391 A-allele carriers showed higher plasma adiponectin and lower total cholesterol levels when compared to G/G homozygotes. Approximately 32 months post-surgery, a mean reduction of 35% in BMI and an important improvement in metabolic profiles were observed. In addition, for the ADIPOQ -11377 polymorphism, a higher decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiponectin; Polymorphism; Obesity; Lipid profile; Bariatric surgery.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500736

Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)
Provedor de dados: 74
Autores: Otaviano,Antonio Roberto; Tonhati,Humberto; Sena,Janete Aparecida Desidério; Cerón Muñoz,Mario Fernando.
Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with changes in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffaloes; Polymorphism; PCR-RFLP; SSCP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200010

Association study of folate-related enzymes (MTHFR, MTR, MTRR) genetic variants with non-obstructive male infertility in a Polish population
Provedor de dados: 74
Autores: Kurzawski,Mateusz; Wajda,Anna; Malinowski,Damian; Kazienko,Anna; Kurzawa,Rafal; Drozdzik,Marek.
Spermatogenesis is a process where an important contribution of genes involved in folate-mediated one-carbon metabolism is observed. The aim of the present study was to investigate the association between male infertility and the MTHFR (677C &gt; T; 1298A &gt; C), MTR (2756A &gt; G) and MTRR (66A &gt; G) polymorphisms in a Polish population. No significant differences in genotype or allele frequencies were detected between the groups of 284 infertile men and of 352 fertile controls. These results demonstrate that common polymorphisms in folate pathway genes are not major risk factors for non-obstructive male infertility in the Polish population.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: MTHFR; MTR; MTRR; Polymorphism; Infertility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100042

Evaluation of the association between the TAS1R2 and TAS1R3 variants and food intake and nutritional status in children
Provedor de dados: 74
Autores: Melo,Silvia V.; Agnes,Grasiela; Vitolo,Márcia R.; Mattevi,Vanessa S.; Campagnolo,Paula D.B.; Almeida,Silvana.
Abstract Taste perception plays a key role in determining individual food preferences and dietary habits and may influence nutritional status. This study aimed to investigate the association of TAS1R2 (Ile191Val - rs35874116) and TAS1R3 (-1266 C/T - rs35744813) variants with food intake and nutritional status in children followed from birth until 7.7 years old. The nutritional status and food intake data of 312 children were collected at three developmental stages (1, 3.9 and 7.7 years old). DNA was extracted from blood samples and the polymorphisms were analyzed by real-time polymerase chain reactions (qPCR) using hydrolysis probes as the detection method. Food intake and nutritional status were compared among individuals with different single nucleotide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food intake; Polymorphism; TAS1R2 gene; TAS1R3 gene; Taste receptors.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300415

Replication of TCF7L2 rs7903146 association with type 2 diabetes in an Iranian population
Provedor de dados: 74
Autores: Amoli,Mahsa M.; Amiri,Parvin; Tavakkoly-Bazzaz,Javad; Charmchi,Elham; Hafeziyeh,Jila; Keramatipour,Mohammad; Abiri,Maryam; Ranjbar,Shirin Hasani; Larijani,Bagher.
The transcription factor 7-like 2 gene (TCF7L2) rs7903146 T allele is constantly associated with Type 2 diabetes in various populations and ethnic groups. Nevertheless, this has not been observed in two studies involving Arab populations. The aim of the present study was to investigate the association between TCF7L2 rs7903146 in an Iranian population. Type 2 diabetes patients (N = 258) and normal healthy control subjects (N = 168) from the same area, were examined. The ARMS-PCR (Amplification Refractory Mutation System) technique, subsequently validated by direct sequencing, was used for genotyping. Allele and genotype frequencies were significantly different between patients and controls TT vs. CT + CC [p 0.0081 OR 3.4 95%CI (1.27-11.9)] and T vs. C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TCF7L2; Gene; Polymorphism; T2DM.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300010

Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies
Provedor de dados: 74
Autores: Lima,Vitor Lopes de Abreu; Seki,Homiko Abreu; Rumjanek,Franklin David.
Eleven samples of wheat (Triticum aestivum) from different Brazilian cultivars and six American varieties were compared for polymorphism, using primers for nine different STR loci. STR analysis of DNA from single grains of the Brazilian varieties showed that for most loci there was very little intra-cultivar polymorphism. The polymorphic variation observed for Brazilian cultivars was similar to that seen in the American varieties. For the Brazilian cultivars PCR analysis could be performed on only one half of a grain. The American samples required more seeds for analysis. The nucleotide sequences of five amplified microsatellites selected at random from the Brazilian samples were also determined and compared to those of the Chinese Spring variety. Although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Brazilian; Cultivars; DNA; STR; Polymorphism.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300021

Frequency of adult type-associated lactase persistence LCT-13910C/T genotypes in the Czech/Slav and Czech Roma/Gypsy populations
Provedor de dados: 74
Autores: Hubácek,Jaroslav A.; Adámková,Věra; Šedová,Lenka; Olišarová,Věra; Adámek,Václav; Tóthová,Valérie.
Abstract Lactase non-persistence (leading to primary lactose intolerance) is a genetically dependent inability to digest lactose in adulthood. As part of the human adaptation to dairying, the human lactase LCT-13910C/T mutation (which propagates adult expression of lactase) developed, spread and participated in the adaptation to dairying. This variant is associated with lactase activity persistence, and its carriers are able to digest lactose. We compared the frequencies of lactase 13910C/T (rs4988235) genotypes in Czechs/Slavs (N = 288) and Czech Gypsies/Roma (N = 300), two ethnically different groups where this polymorphism has not yet been analysed. Allelic frequencies significantly differed between the populations (p &lt; 0.0001). In Czechs/Slavs, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactose; Lactase persistence; Polymorphism; Czech/Slav; Czech Gypsy/Roma.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300450

Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
Provedor de dados: 74
Autores: Santos,Ana Maria Pujol Vieira dos; Matsumura,Aida T. Santos; Van Der Sand,Sueli Teresinha.
The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drechslera tritici-repentis; RAPD; DNA; Polymorphism; Genetic diversity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200020

Evaluation of polymorphism in ten microsatellite loci in Uruguayan sheep flocks
Provedor de dados: 74
Autores: Tomasco,Ivanna; Wlasiuk,G.; Lessa,E.P.
The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Polymorphism; Microsatellites.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100008

A meta-analysis of ABCG2 gene polymorphism and non-small cell lung cancer outcomes
Provedor de dados: 74
Autores: Fu,Lei; Wang,Rong; Yin,Ling; Shang,Xiaopu; Zhang,Runtong; Zhang,Pengjun.
Abstract We aimed to analyze the correlation between ABCG2 gene polymorphisms of 34 GG/(GA + AA) loci, 421 CC/(AC + AA) loci, and non-small cell lung cancer (NSCLC) therapeutic effects via meta-analysis. With key words, the databases PubMed and EMBASE were searched for clinical studies on ABCG2 polymorphism and NSCLC. RR and 95% CIs were used to compute combined effects, followed by heterogeneity testing. Publication bias was examined using the funnel plot method. Review Manager 5.3 software was used for the meta-analysis. Ten studies were included. No evidence of heterogeneity exists in these studies. The results indicate that two polymorphic loci of ABCG2 gene (34 G&gt;A, and 421 C&gt;A) had no relationship with the curative effect of chemotherapy for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABCG2; Polymorphism; Non-small cell lung cancer; Chemotherapy; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500105

Mitochondrial DNA D-loop sequence variation among 5 maternal lines of the Zemaitukai horse breed
Provedor de dados: 74
Autores: Cothran,E. Gus; Juras,Rytis; Macijauskiene,Vale.
Genetic variation in Zemaitukai horses was investigated using mitochondrial DNA (mtDNA) sequencing. The study was performed on 421 bp of the mitochondrial DNA control region, which is known to be more variable than other sections of the mitochondrial genome. Samples from each of the remaining maternal family lines of Zemaitukai horses and three random samples for other Lithuanian (Lithuanian Heavy Draught, Zemaitukai large type) and ten European horse breeds were sequenced. Five distinct haplotypes were obtained for the five Zemaitukai maternal families supporting the pedigree data. The minimal difference between two different sequence haplotypes was 6 and the maximal 11 nucleotides in Zemaitukai horse breed. A total of 20 nucleotide differences compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D-loop; Equus caballus; Phylogeny; Polymorphism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500006

MHC class I loci of the Bar-Headed goose (Anser indicus)
Provedor de dados: 74
Autores: Liang,Qinglong; Wei,Lei; Wang,Xinwei; He,Hongxuan.
MHC class I proteins mediate functions in anti-pathogen defense. MHC diversity has already been investigated by many studies in model avian species, but here we chose the bar-headed goose, a worldwide migrant bird, as a non-model avian species. Sequences from exons encoding the peptide-binding region (PBR) of MHC class I molecules were isolated from liver genomic DNA, to investigate variation in these genes. These are the first MHC class I partial sequences of the bar-headed goose to be reported. A preliminary analysis suggests the presence of at least four MHC class I genes, which share great similarity with those of the goose and duck. A phylogenetic analysis of bar-headed goose, goose and duck MHC class I sequences using the NJ method supports the idea...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MHC I; Anser indicus; Polymorphism; Phylogenetic analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300031

Polymorphisms of the DNA repair genes XRCC1 and XRCC3 in a Brazilian population
Provedor de dados: 74
Autores: Duarte,Márcia Cristina; Colombo,Jucimara; Rossit,Andréa Regina Baptista; Silva,Ana Elizabete.
In several DNA repair genes, polymorphisms may result in reduced repair capacity, which has been implicated as a risk factor for various types of cancer. The frequency of the polymorphic alleles varies among populations, suggesting an ethnic distribution of genotypes. We genotyped 300 healthy Southeastern Brazilian individuals (262 of European ancestry and 38 of African ancestry) for polymorphisms of codons 194 and 399 of the XRCC1 base excision repair pathway gene and of codon 241 of the XRCC3 homologous recombination repair pathway gene. The allele frequencies were 0.07 for the Arg194Trp and 0.33 for the Arg399Gln codons of the XRCC1 gene and 0.35 for the Thr241Met codon of the XRCC3 gene. The genotypic frequencies were within Hardy-Weinberg equilibrium....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: DNA repair; XRCC1; XRCC3; Polymorphism; Ethnic variability.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300011

Analysis of the CCR5 gene coding region diversity in five South American populations reveals two new non-synonymous alleles in Amerindians and high CCR5*D32 frequency in Euro-Brazilians
Provedor de dados: 74
Autores: Boldt,Angelica B.W.; Culpi,Lodércio; Tsuneto,Luiza T.; Souza,Ilíada R.; Kun,Jürgen F.J.; Petzl-Erler,Maria Luiza.
The CC chemokine receptor 5 (CCR5) molecule is an important co-receptor for HIV. The effect of the CCR5*D32 allele in susceptibility to HIV infection and AIDS disease is well known. Other alleles than CCR5*D32 have not been analysed before, neither in Amerindians nor in the majority of the populations all over the world. We investigated the distribution of the CCR5 coding region alleles in South Brazil and noticed a high CCR5*D32 frequency in the Euro-Brazilian population of the Paraná State (9.3%), which is the highest thus far reported for Latin America. The D32 frequency is even higher among the Euro-Brazilian Mennonites (14.2%). This allele is uncommon in Afro-Brazilians (2.0%), rare in the Guarani Amerindians (0.4%) and absent in the Kaingang...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR5; Brazilian; Amerindian; HIV; Polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100002

DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle
Provedor de dados: 74
Autores: Choudhary,Vivek; Kumar,Pushpendra; Bhattacharya,Tarun K.; Bhushan,Bharat; Sharma,Arjava.
Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle. We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 403 cattle belonging to various breeds of Bos indicus (Hariana, Sahiwal, Gir and Nimari cattle), Bos taurus (Holstein Friesian (HF) and Jersey cattle) as well as Bos taurus x Bos indicus crossbreds (½ HF x ½ Hariana). In all the cattle we amplified two regions of the leptin gene, a 522 bp fragment comprising the partial intron 2 and exon 3 and another 94 bp fragment consisting of part of exon 2. Digestion of 522 bp PCR products with the BsaAI restriction enzyme revealed three genotypes in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leptin gene; PCR-RFLP; Polymorphism; Bos indicus; Bos taurus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500014

Allozyme comparison of two populations of Rineloricaria (Siluriformes, Loricariidae) from the Ivaí River, upper Paraná River basin, Brazil
Provedor de dados: 74
Autores: Limeira,Daniel M.; Renesto,Erasmo; Zawadzki,Cláudio H.
Two allopatric morphotypes of the genus Rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, R. pentamaculata, from the Keller River in the middle stretch of the Ivaí River basin and the other, R. aff. pentamaculata, from the São João River in the upper portion of the Ivaí River basin. The morphotype from the São João River was collected upstream from the São João waterfall, which is about 80 m deep. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, which allowed to score 22 loci. Only loci Aat-2, Est-3 and Mdh-C showed polymorphism. The two samples differed in allele frequencies at the three polymorphic loci. The average expected heterozygosity for all loci was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Allozymes; Fish genetics; Genetic distance; Polymorphism; Loricariidae; Pisces.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200034

Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study
Provedor de dados: 74
Autores: Hubacek,Jaroslav A.; Lanska,Vera; Adamkova,Vera.
Smoking has a significant heritable component of approximately 30-60%. Recent genome wide association studies have identified single nucleotide polymorphisms (SNPs) within the nicotinic cholinergic receptor subunits 3 (rs578776), 5 (rs16969968) and β3 (rs6474412), which are associated with nicotine dependence in Western European populations. To analyze the association in a Czech population, we genotyped 1,191 males and 1,368 females (post-MONICA study). The WHO protocol was used to examine smoking status and the number of cigarettes smoked per day. There were 32.1% current and 27.6% past smokers among the males and 22.5% current and 13.8% past smokers among the females. We have not confirmed the original results: the SNPs rs16969968 (p = 0.07), rs578776 (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholinergic receptors; Polymorphism; Smoking.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500004

Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds
Provedor de dados: 74
Autores: Mazzi,Carmen Maria; Ferro,Jesus Aparecido; Ferro,Maria Inês Tiraboschi; Savino,Vicente José Maria; Coelho,Antonio Augusto Domingos; Macari,Marcos.
The promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (Hubbard-Pettersen), a breed selected for weight gain (PP1) and a non-selected breed (naked-neck Label Rouge). The naked neck gene (Naked neck, Na), which reduces feathering in birds and is thus related to heat resistance, was present in both PP1 and Label Rouge breeds. Genomic DNA was restricted with PstI and Southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. PCR-SSCP analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler chickens; Hsp70 gene; Naked neck (Na) gene; Polymorphism; SSCP.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300010

Paraplexia Enzoótica dos ovinos: características gerais da Scrapie.
Provedor de dados: 14
Autores: SANCHES, C. C.; ROSINHA, G. M. S.; SOARES, C. O.; GALVÃO, C. E.; ARAUJO, F. R.; FEIJO, G. L. D.
A scrapie, enfermidade que acomete ovinos, faz parte do grupo de doenças priônicas denominadas como encefalopatias espongiformes transmissíveis (EETs), na qual estão incluídas a encefalopatia espongiforme bovina (EEB) e, em humanos, a kuru, doença de Gerstmann-Straussle-Scheinker (GSS), e doença de Cretzfeldt-Jakob (CJD). Estas enfermidades são fatais, caracterizadas por sintomas neurodegenerativos incluindo demência e mudança de comportamento, ataxia, incoordena ção muscular, prurido, entre outros. As doenças priônicas são causadas pelo acúmulo indiscriminado da proteína PrPC que por alteração em seu sítio de codificação, sofre modificações químicas, transformando sua estrutura inicialmente primária (PrPc), em estrutura terciária, denominada PrPSc, forma...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Ovino; Polimorfismo; Sanidade Animal.; Polymorphism; Sheep.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/970478

High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Rosa, Rafael D.; Alonso, Pascal; Santini, Adrien; Vergnes, Agnes; Bachere, Evelyne.
We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in...
Tipo: Text
Palavras-chave: Mollusk bivalve; Antimicrobial peptide; Polymorphism; Gene copy number variation; High-throughput microfluidic RT-qPCR; Vibrio infection.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00239/35009/33545.pdf

A high load of non-neutral amino-acid polymorphisms explains high protein diversity despite moderate effective population size in a marine bivalve with sweepstakes reproduction
Provedor de dados: 5
Autores: Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas.
Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the...
Tipo: Text
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf

Herpèsvirus infectant les bivalves marins : détection, génome et transmission
Provedor de dados: 5
Autores: Arzul, Isabelle.
Since 1991, high mortality rates of larvae and juveniles of different bivalve mollusc species associated with herpesvirus infections have been reported in different countries. The aim of this study was to determine if more than one helpesvirus species was responsible for these infections or just one species exhibiting polymorphism. The investigations concentrated on the analysis of the virus genome, studies of virus transmission in different mollusc species and on the potential role of bivalve adults in the occurrence of infections. The analysis of various target sequences within the virus genome present in infected larvae samples demonstrated the presence of polymorphism. More notably a variant of the virus was identified and the variation appears...
Tipo: Text
Palavras-chave: Herpèsvirus; Bivalves; Transmission; Polymorphisme; OsHV-1; Diagnostic; Herpesviruses; Bivalvia; Transmission; Polymorphism; OsHV-1; Diagnostic.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00105/21675/19253.pdf

A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections
Provedor de dados: 5
Autores: Rosa, Rafael Diego; De Lorgeril, Julien; Tailliez, Patrick; Bruno, Roman; Piquemal, David; Bachere, Evelyne.
Background: The complex balance between environmental and host factors is an important determinant of susceptibility to infection. Disturbances of this equilibrium may result in multifactorial diseases as illustrated by the summer mortality syndrome, a worldwide and complex phenomenon that affects the oysters, Crassostrea gigas. The summer mortality syndrome reveals a physiological intolerance making this oyster species susceptible to diseases. Exploration of genetic basis governing the oyster resistance or susceptibility to infections is thus a major goal for understanding field mortality events. In this context, we used high-throughput genomic approaches to identify genetic traits that may characterize inherent survival capacities in C. gigas. Results:...
Tipo: Text
Palavras-chave: Marine invertebrate; Mollusk bivalve; Mass mortality; Transcriptome-wide analysis; Digital gene expression; Microfluidic qPCR; Survival signature; Polymorphism; Gene copy number; Survival predictive biomarkers.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00095/20624/18262.pdf

Genetic characterisation of oyster populations along the north-eastern coast of Tunisia
Provedor de dados: 5
Autores: Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie.
The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster 16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples, which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is now...
Tipo: Text
Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf

Reduced female gene flow in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Diaz Almela, F; Boudry, Pierre; Launey, Sophie; Bonhomme, Francois; Lapegue, Sylvie.
The geographical structure of 15 natural populations of the flat oyster (Ostrea edulis L.) was assessed by single-strand conformation polymorphism (SSCP) of a 313-base-pair (bp) fragment of the mitochondrial 12S-rRNA gene. Fourteen haplotypes were observed, with one being dominant in the Mediterranean samples and another one in the Atlantic populations. The geographically extreme populations sampled in Norway and the Black Sea appeared differentiated by exhibiting the dominance of a third group of haplotypes. The results were compared to available microsatellite data at five loci. The Atlantic/Mediterranean differentiation pattern was qualitatively the same with both types of markers, confirming an isolation-by-distance pattern. The average mitochondrial...
Tipo: Text
Palavras-chave: Ostrea edulis; Polymorphism; Flat oyster; Population; Gene.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-680.pdf

Les gènes de l'amylase chez l'huître creuse Crassostrea gigas : transmission et mise en évidence des pressions sélectives
Provedor de dados: 5
Autores: Rambelo-ramanitra, Seheno.
Abnormal massive mortalities of oysters Crassostrea gigas during the estival period are a phenonmenon described in the literature since forty years. A certain number of studies show that it could exist a genectic base with these mortalities. In front of the economic interest that the oyster production in France represents, IFREMER set up program MOREST, gathering the fiels of physiology, pathology and the genetics. This study in interested in rare genotypes, determined by PCR-RFLP, presenting three bands for one or two A and B amylase genes, met in the families MOREST and the polymorphic population for amylase genes, made up starting from crossing of the hierarchical type. It aims at making a follow-up of the segregation of the alleles at the progeny, as...
Tipo: Text
Palavras-chave: Crassostrea gigas; Extraction d'ADN; Amylase; Polymorphisme; Génotypes; Crassostrea gigas; DNA Extraction; Amylase; Polymorphism; Genotypes.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00371/48230/48364.pdf

Polymorphisme de Biddulphia sinensis greville dans le bassin de Marennes-Oléron.
Provedor de dados: 5
Autores: Le Borgne-david, Annick.
The plankton catches carried out in the Marennes-Oléron basin during the summer of 1971, in order to monitor the extent and changes of oyster larvae emissions, have also served at the same time as phytoplanktonic studies.
Tipo: Text
Palavras-chave: Apophyse; Spines; Polymorphism; Diatom; Plankton.
Ano: 1972
URL: http://archimer.ifremer.fr/doc/1972/publication-2051.pdf

Comportements cellulaires et régulation génétique au cours des réactions d'immunité innée chez la moule Mytilus galloprovincialis
Provedor de dados: 5
Autores: Li, Hui.
The mytiliculture is one of the most important aquaculture in the world, however, little is known on the mussel immune system, which plays a critical role in case of infectious diseases. Like all the other invertebrates, our model, the Mediterranean mussel Mytilus galloprovincialis, possesses only an innate immune system considered to be primitive and non-specific. In the present study, our results showed that the mussel immune system (i) is capable of responding differently to a variety of stimulations, suggesting a specific recognition process; (ii) reacts differently according to the geographic origin of the animals, showing an adaptation to their own environment; (iii) is influenced by the season, which explains the seasonal mortalities; (iv) presents...
Tipo: Text
Palavras-chave: Mytilus; Haemocyte; Antimicrobial peptides; Polymorphism; Genetic regulation; Innate immunity; Mytilus; Hémocyte; Peptides antimicrobiens; Polymorphisme; Régulation génétique; Immunité innée.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-6241.pdf

French scallops: A new host for ostreid herpesvirus-1
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text
Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf

Identifying the viral genes encoding envelope glycoproteins for differentiation of Cyprinid herpesvirus 3 isolates
Provedor de dados: 5
Autores: Han,; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano, Jr.; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang.
Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA,...
Tipo: Text
Palavras-chave: Cyprinid herpes virus 3; Glycoprotein gene; Koi herpesvirus; Microsatellite; Polymorphism; Phylogenetic tree.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00118/22940/20768.pdf

Etude de la variabilité génétique de populations naturelles d'huîtres creuses Crassostrea gigas (Thunberg) du littoral Atlantique Français
Provedor de dados: 5
Autores: Gras, Marie-paule; Gras, Paul.
The aductor muscle proteins of Japaneses oysters C. gigas collected from three different geographical wild stocks were studied by acrylamid gel electrophoreseis. The first results reveal a biochemical polymorphism of a protein fraction
Tipo: Text
Palavras-chave: Crassostrea gigas; Oysters; Genetic; Polymorphism; Genetic population; Crassostrea gigas; Huîtres; Génétique; Polymorphisme; Populations naturelles.
Ano: 1982
URL: http://archimer.ifremer.fr/doc/1982/acte-2952.pdf

Polymorphism C373G of the PECAM-1 Gene in Chilean Subjects with Coronary Disease and Controls
Provedor de dados: 77
Autores: Herrera,Christian L; Jaramillo,Priscilla C; Lanas,Fernando; Salazar,Luis A.
The platelet endothelial cell adhesion molecule-1 (PECAM-1), a 130-kDa membrane glycoprotein, is expressed on the surface of monocytes, some T-lymphocyte subsets, neutrophils, platelets and endothelial cells. PECAM-1 plays a key role in the transendothelial migration of circulating leukocytes during vascular inflammation. The aim of the present investigation was to evaluate the association between the C373G polymorphism of the PECAM-1 gene and coronary artery disease in Chilean subjects. A total of 220 individuals were investigated (112 cases and 108 controls). The presence of coronary artery disease was confirmed by angiography (Stenosis &gt;70%). The C373G polymorphism was detected by polymerase chain reaction followed enzymatic restriction. The genotype...
Tipo: Journal article
Palavras-chave: Aterosclerosis; PECAM-1; Polymorphism.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200031

Hemoglobin Polymorphism and Morphometrical Correlates in the West African Dwarf Sheep of Nigeria
Provedor de dados: 77
Autores: Akinyemi,M. O; Salako,A. E.
Red cells extracted from blood samples taken by jugular venipuncture from a total of thirty-six traditionally managed adult West African Dwarf rams and ewes were subjected to starch gel electrophoresis, stained to reveal the activities of different allelemorphs at hemoglobin locus and analyzed. The studies was aimed at examining genetic variation as well as pattern of segregation at the locus and unveil possible association that might exist between the phenotypes and selected zoometrical traits. From the three theoretically phenotypes expected to be produced in the population by the two co-dominant alleles identified, only two: HbAA and HbAB were observed. The observed genotype frequencies conformed to Hardy-Weinberg's equilibrium. Proportion of...
Tipo: Journal article
Palavras-chave: Hemoglobin alleles; West African Dwarf; Metric traits; Polymorphism.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100029

Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
Provedor de dados: 46
Autores: Ripoli,M.V; Rogberg-Muñoz,A; Liron,J.P; Giovambattista,G.
Several methods such as PCR-RFLP, OLA, DNA sequencing, PCR-SSCP and ARMS-PCR have been developed to detect the allelic variation at substitutions K232A of DGAT1, GH6.1 of GH, F279Y of GHR, R4C of LEP, I74V of FABP4, and at the transition in the TG 5´ leader sequence. Most of these methods are manual processes and therefore increase the time spent on the assay and limit the number of animals analyzed. Herein, we describe the development of pyrosequencing-based methods for the bovine DGAT1, GH, GHR, LEP, FABP4 and TG genes, whose polymorphisms have been associated with variation in carcass composition. This method was validated by analyzing DNA samples belonging to the Aberdeen Angus and Hereford breeds previously typed by PCR-SSCP, PCR-RFLP and/or DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine molecular markers; Polymorphism; Pyrosequencing; Marbling.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300005

Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected withHelicobacter pylori
Provedor de dados: 80
Autores: Caleman Neto,Agostinho; Rasmussen,Lucas T; Labio,Roger W de; Queiroz,Valdeir F de; Smith,Marília de AC; Viani,Gustavo A; Payão,Spencer LM.
Background : Epidemiological investigations have indicated thatHelicobacter pyloriinduces inflammation in the gastric mucosa regulated by several interleukins. The genesIL1BandIL8are suggested as key factors in determining the risk of gastritis. The aim of this paper was to evaluate the association of gene polymorphism of interleukin-1 and interleukin-8 with chronic gastrits inH. pyloriinfected patients. A total of 60 patients underwent endoscopic procedure. Biopsy samples were collected for urease test, histopathological and molecular exams. The DNA of theses samples was extracted for detection ofH. pyloriand analysis of the genes mentioned above. Patients with gastritis had a higher frequency ofH. pylori-positive samples.Results : H. pyloriwas detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Polymorphism; Interleukin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200327

Redescription of Arcturides cornutus Studer, 1882 from the Crozet Islands and Prince Edward Island (Crustacea, Isopoda, Valvifera)
Provedor de dados: 16
Autores: Park, Ja-Yang.
A redescription of the polymorphic arcturid species Arcturides cornutus from the Crozet Islands and Prince Edward Island is presented. The polymorphism of specimens from various regions and the systematic position of Pseudidotheinae, to which Arcturides cornutus belongs, are discussed.
Tipo: Article / Letter to the editor
Palavras-chave: Crustacea; Valvifera; Pseudidotheinae; Polymorphism.
Ano: 1996
URL: http://www.repository.naturalis.nl/record/505497

Alelle number and heterozigosity for microsatellite loci in different stingless bee species (Hymenoptera: Apidae, Meliponini)
Provedor de dados: 82
Autores: Francisco,Flávio de O.; Brito,Rute M.; Arias,Maria C.
In the present study we compare genetic characteristics (allele diversity and observed heterozygosity) of microsatellite loci, from three stingless bee species (Plebeia remota Holmberg, Partamona mulata Moure In Camargo and Partamona helleri Friese), amplified by using heterospecific primers originally designed for Melipona bicolor Lepeletier and Scaptotrigona postica Latreille. We analyzed 360 individuals of P. remota from 72 nests, 58 individuals of P. mulata from 58 nests, and 47 individuals of P. helleri from 47 nests. The three species studied showed low level of polymorphism for the loci amplified with primers derived from M. bicolor. However, for the loci amplified with primers derived from S. postica, only P. remota presented low level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plebeia remota; Partamona mulata; Partamona helleri; Heterozygosity; Polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000500011

Two New Morphs of the Southern Green Stink Bug, Nezara viridula (L.) (Heteroptera: Pentatomidae), in Brazil
Provedor de dados: 82
Autores: VIVAN,LÚCIA M.; PANIZZI,ANTÔNIO R.
During March-June 2001, surveys of the southern green stink bug, Nezara viridula (L.), were conducted on soybean [Glycine max (L.) Merrill] fields in the Southern Region of Brazil. Colonies of the bug were maintained in the laboratory at Embrapa Soja in Londrina, PR. Two new morphs of N. viridula were obtained.The first, green-yellowish, was captured in the field in the Londrina area. The second, with the anterior margins of the pronotum and head yellow, with the rest of the body green-yellowish, was obtained in the laboratory.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Glycine max; Polymorphism; Color morphs.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000300020

Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
Provedor de dados: 82
Autores: Moretto,Geraldo; Arias,Maria C.
The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restriction enzyme; Polymorphism; PCR+RLFP; MtDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004

Genetic variability of native populations of Wasmannia Forel (Hymenoptera: Formicidae) and their biogeographical implications
Provedor de dados: 82
Autores: Souza,Ana L B; Tavares,Mara G; Serrão,José E; Delabie,Jacques H C.
Wasmannia auropunctata (Roger) and Wasmannia rochai Forel are economically important ants in the Southeast and Southwest regions of Bahia State, Brazil. Randomly Amplified Polymorphic DNA (RAPD) markers were used to distinguish and analyze the genetic variability of populations of both species from Ilhéus, Jequié, BA and French Guyana. The genetic distances among W. auropunctata and W.rochai populations (55.8-71.4%) suggest genetic differentiation among them. Wasmannia auropunctata populations from sugarcane and banana plantations in Jequié were the most distant genetically (30.1-46.3%) and may represent populations restricted to isolated fragments. The high genetic distances among W. auropunctata populations from CEPLAC experimental areas, in Ilhéus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: W. auropunctata; RAPD; Polymorphism; Heterozygosity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000300013

Chromosomal variability in the wild ornamental species of Symphysodon (Perciformes: Cichlidae) from Amazon
Provedor de dados: 83
Autores: Mesquita,Débora R.; Porto,Jorge I. R.; Feldberg,Eliana.
Cytogenetic studies were conducted on three discus species which inhabit the Amazon in Brazil: Symphysodon haraldi from Manacapuru, S. aequifasciatus from Tefé and S. discus from Barcelos. All individuals showed 2n=60 chromosomes, most of them biarmed. No sexual chromosomal heteromorphism was verified. However, different karyotypic formulae, owing to the presence of subtelocentric chromosomes, were verified for S. aequifasciatus and S. discus. One of the karyotypic formulae from S. aequifasciatus (cytotype 2) differs from the others, due to one of the homologues in the first chromosome pair being significantly larger than the other. A large variability was observed toward the nucleolar organizer regions (NOR) of S. haraldi and S. aequifasciatus. Although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Discus; Cytogenetics; Polymorphism; Microchromosomes; Multiple NORs.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000200005

Genetic variability in three species of Gymnotus Linnaeus, 1758 (Gymnotiformes: Gymnotidae) from Caracu stream of the upper Paraná River basin, Brazil
Provedor de dados: 83
Autores: Rezende,Judy Ruiz; Renesto,Erasmo; Zawadzki,Cláudio Henrique.
The genetic variability of three Gymnotus species from the Caracu stream, a small tributary of the left margin of Paraná River (Brazilian upper Paraná River floodplain), was estimated with data of 17 putative allozyme loci, which were obtained by using corn starch gel electrophoresis of 10 enzymatic systems: Aspartate aminotransferase (E. C. 2.6.1.1), Alcohol dehydrogenase (E. C. 1.1.1.1), Esterase (E. C. 3.1.1.1), Glucose dehydrogenase (E. C. 1.1.1.118), Glycerol-3-phosphate dehydrogenase (E. C. 1.1.1.8), Isocitrate dehydrogenase (E. C. 1.1.1.42), L-Lactate dehydrogenase (E. C. 1.1.1.27), Malate dehydrogenase (E. C. 1.1.1.37), Superoxide dismutase (E. C. 1.15.1.1) and Sorbitol dehydrogenase (E. C. 1.1.1.14). The genetic diversity was estimated as He =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Genetic distance; Allozyme; Heterozygosity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400010

The Effect of Single Nucleotide Polymorphisms in the Tumor Necrosis Factor-α Gene on Reproductive Performance and Immune Function in Dairy Cattle
Provedor de dados: 19
Autores: KAWASAKI, Yurie; AOKI, Yuka; MAGATA, Fumie; MIYAMOTO, Akio; KAWASHIMA, Chiho; HOJO, Takuo; OKUDA, Kiyoshi; SHIRASUNA, Koumei; SHIMIZU, Takashi; 宮本, 明夫; 清水, 隆.
The present study aimed to assess the effect of polymorphisms in the tumor necrosis factor α (TNF-α) promoter (A/A, A/G and G/G) and exons (T/T, T/C and C/C) on immune function and reproductive performance in dairy cows. The occurrence of the first postpartum ovulation within 3 weeks in the cows with the TNF-α promoter A/G and G/G genotypes was higher than in the A/A group. Among the different TNF-α exon genotypes, the occurrence of early first postpartum ovulation was higher in the T/C and C/C genotype groups than in the T/T group. Single nucleotide polymorphisms (SNPs) in the TNF-α gene did not affect the rate of artificial insemination (AI) or duration from parturition to next conception (days open). The apoptosis rate of polymorphonuclear leukocytes...
Palavras-chave: Cattle; Immune function; Polymorphism; Reproductive performance; Tumor necrosis factor α (TNF-α).
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3934

Relationships Between the First Ovulation Postpartum and Polymorphism in Genes Relating to Function of Immunity, Metabolism and Reproduction in High-producing Dairy Cows
Provedor de dados: 19
Autores: SHIRASUNA, Koumei; KAWASHIMA, Chiho; MURAYAMA, Chiaki; AOKI, Yuka; MASUDA, Yutaka; KIDA, Katsuya; MATSUI, Motozumi; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 川島, 千帆; 増田, 豊; 木田, 克弥; 松井, 基純; 清水, 隆; 宮本, 明夫.
The decrease in fertility and conception rates of high-producing dairy cows is one of the major negative impacts for today's producers. The recovery of ovarian activity postpartum is affected by the status of immunity, metabolism and reproduction and plays a critical role in subsequent fertility after parturition in the cow. In the present study we investigated the relationships between polymorphisms in genes relating to the above functions and the first postpartum ovulation as a marker of the recovery of ovarian function in the cow. In immune function related-factors, the occurrence of first postpartum ovulation within 3 weeks in the C/C genotypes of tumor necrosis factor α (TNFα) exon (55.4%) and the A/G genotypes of TNFα promoter (55.4%) was...
Palavras-chave: Cow; Ovulation; Polymorphism; Tumor necrosis factor α (TNFα).
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2990

Arachis hoehnei, the probable B genome donor of Arachis hypogaea based on crossability, cytogenetical and molecular studies
Provedor de dados: 30
Autores: Mallikarjuna, N.
Palavras-chave: Genomes; Hybrids; Polyploidy; Cytogenetics; DNA; Groundnuts; Mosses; Genetic polymorphism; Polymorphism; Genetic improvement.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3177

Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea)
Provedor de dados: 30
Autores: Luu M Cuc.
Palavras-chave: Genomes; Genetic structures; Polymorphism; Alleles; DNA; Genotypes; PCR; Cloning; Locus; Groundnuts.
Ano: 2008
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3317

Development of new simple sequence repeat markers for pearl millet
Provedor de dados: 30
Autores: Yadav, O.P.
Palavras-chave: Locus; DNA; Genetic structures; PCR; Millets; Polymorphism; Genomes; Biotechnology; Breeds (animals); Melting.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3309

Genetic structure, diversity, and allelic richness in composite collection and reference set in chickpea (Cicer arietinum L.)
Provedor de dados: 30
Autores: Upadhyaya, H.D.
Palavras-chave: Alleles; Chickpeas; Locus; Genomes; Polymorphism; Genes; Genetic structures; DNA; PCR; Peas.
Ano: 2008
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3334

Molecular genetic relationships among Arachis diogoi and A. chiquitana accessions
Provedor de dados: 30
Autores: Mallikarjuna, N.,( Nalini Mallikarjuna ).
Palavras-chave: DNA; Polymorphism; RAPD; Genotypes; Locus; Genetic structures; Groundnuts; Hybrids; Nitrogen; Mass media.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3294

Development of cost-effective SNP assays for chickpea genome analysis and breeding
Provedor de dados: 30
Autores: Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genomes; Polymorphism; Genotypes; Enzymes; Nucleotides; Chickpeas; DNA; Bioinformatics; Genetic structures; Genes.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3282

Extending the repertoire of microsatellite markers for genetic linkage mapping and germplasm screening in chickpea
Provedor de dados: 30
Autores: Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genetic structures; Chickpeas; Genomes; Cloning; Chromosome mapping; Plasmids; Polymorphism; DNA; Genotypes; Genetic markers.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3338

The advertisement call, color patterns and distribution of Ischnocnema izecksohni (Caramaschi and Kisteumacher, 1989) (Anura, Brachycephalidae)
Provedor de dados: 84
Autores: Taucce,Pedro P. G.; Leite,Felipe S. F.; Santos,Patrícia S.; Feio,Renato N.; Garcia,Paulo C. A.
Ischnocnema izecksohni inhabits the gallery forests from the Quadrilátero Ferrífero, Southern Espinhaço range, state of Minas Gerais, southeastern Brazil, and it is considered endemic to this region. Its closest related species is I. nasuta according to the original description. We describe the advertisement call of I. izecksohni based on specimens recorded and collected at the municipality of Nova Lima, state of Minas Gerais, distant about 10 km straight line from its type locality. The advertisement call consists of a group of notes emitted sporadically without a regular interval between the calls. Call duration (n = 36 calls in four individuals) ranged from 1.03 to 1.85 s (= 1.52 ± 0.21 s) and the call rise time from 0.66 to 1.52 s (= 1.16 ± 0.25 s),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vocalization; Taxonomy; Polymorphism; Ischnocnema guentheri species series.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492012000900001

Extreme homogeneity among Brazilian wheat genotypes determined by RAPD markers
Provedor de dados: 86
Autores: FREITAS,LORETA BRANDÃO DE; JERUSALINSKY,LEANDRO; BONATTO,SANDRO LUIS; SALZANO,FRANCISCO MAURO.
Random amplified polymorphic DNA markers (RAPD) were used to estimate the variability of 14 genotypes of Brazilian wheat (Triticum aestivum L.), using a set of 50 random 10mer primers. A total of 256 reproducibly scorable DNA amplification products were obtained from 48 of the primers, 83% of which were polymorphic. Genetic distances among genotypes were calculated and a dendrogram and a principal coordinates analysis showing the genetic relationships among them were obtained. Despite the low variability found (average genetic distance of 27%), two groups of genotypes could be identified, which probably reflect how they were formed. Studies such as this one may be important in the planning and development of future improvement programs for this plant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Genetic variation; Polymorphism; Breeding methods.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000001100018

Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus
Provedor de dados: 86
Autores: Curi,Rogério Abdallah; Krauskopf,Monique Marcondes; Hadlich,Janaína Conte; Fortes,Marina Rufino Salinas; Vankan,Dianne Margaret; Silva,Josineudson Augusto II Vasconcelos; Oliveira,Henrique Nunes de; Mota,Marcílio Dias Silveira da.
The objective of this work was to evaluate the effects of single-nucleotide polymorphisms (SNPs) in the genes IGF1 (AF_017143.1:g.198C&gt;T), MSTN (AF_320998.1:g.433C&gt;A), MYOD1 (NC_007313:g.1274A&gt;G) and MYF5 (NC_007303:g.1911A&gt;G) on carcass and meat traits in Nelore (Bos indicus) and Nelore x B. taurus. A total of 300 animals were genotyped and phenotyped for rib eye area (REA), backfat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The effects of allele substitution for each SNP were estimated by regression of the evaluated phenotypes on the number of copies of a particular allele using the general linear model. The polymorphism at IGF1 was non-informative in Nelore animals. In crossbred...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; Candidate gene; Fat deposition; Meat tenderness; Polymorphism.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000200019

Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD
Provedor de dados: 86
Autores: Andrade-Rodríguez,María; Villegas-Monter,Angel; Carrillo-Castañeda,Guillermo; García-Velázquez,Armando.
The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitrofor six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P&lt;0.05) the morphological characteristics of fruitsand the number ofembryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus volkameriana; Rootstock; Embryo culture; Molecular markers; Polymorphism.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000600006

Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls
Provedor de dados: 86
Autores: Mattos,Keiko Kusamura de; Del Lama,Silvia Nassif; Martinez,Mario Luiz; Freitas,Ary Ferreira.
The objective of this study was to obtain genetic marker information in the Gyr breed by analyzing bGH and Pit-1 gene polymorphisms and to verify their association with milk production traits. One sample including 40 Gyr bulls were genotyped at two bGH gene restriction sites (bGH- AluI and bGH-MspI) and at one restriction site in the Pit-1 gene (Pit-1 HinfI). The bGH-MspI(-) allele was favorable for fat milk percentage. The heterozigous Pit-1 HinfI (+/-) bulls were superior for fat milk production, in relation to homozigous Pit-1 HinfI (+/+). The Pit-1 and bGH genes are strong candidates in the dairy cattle QTL search, and zebuine populations are promising samples for this purpose.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cattle; Genetic markers; Polymorphism; Zebu.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000200007

Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars
Provedor de dados: 86
Autores: Ochoa,Elisa del Carmen Martínez; Andrade-Rodríguez,María; Rodríguez,Mario Rocandio; Monter,Angel Villegas.
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangifera indica; Embryo culture; Funiculus; Molecular markers; Polymorphism.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001100010

Germination Ecology of Catchfly (Silene conoidea) Seeds of Different Colors
Provedor de dados: 87
Autores: TANVEER,A.; JAVAID,M.M.; ABBAS,R.N.; ALI,H.H.; NAZIR,M.Q.; BALAL,R.M.; SHAHID,M.A.
ABSTRACT Catchfly (Silene conoidea), an annual herb, is usually recognized as an emerging weed species in Eurasia and North America. The presence of somatic seed polymorphism might aid in the adaptation of this weed in different climatic conditions. We conducted laboratory and greenhouse experiments to study the seed polymorphism and influence of various environmental factors like temperature, salt stress, osmotic stress and burial depth on the germination and emergence characteristics of catchfly. Optimum germination of seeds of all colors was recorded at a temperature of 15 oC. Germination of catchfly seeds of all colors followed decreasing trend as NaCl concentration increased from 50 mM to 200 mM. Seed germination was maximum (87-96%) at 0 MPa but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Catchfly; Germination ecology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100220

Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers
Provedor de dados: 87
Autores: SCHNEIDER,T.; RIZZARDI,M.A.; BRAMMER,S.P.; SCHEFFER-BASSO,S.M.; NUNES,A.L.
ABSTRACT: In view of the rapid evolution of Conyza sumatrensis populations resistant to glyphosate, it is necessary to understand the genetic diversity aimed to improve strategies for managing this weed. We investigated the genetic dissimilarity among 15 biotypes of C. sumatrensis from different geographic regions using microsatellite loci. The biotypes, were cultivated in a greenhouse to obtain vegetal material for DNA extraction. Nineteen microsatellite markers (SSR), were developed for C. sumatrensis biotypes. The genetic dissimilarity was estimated by the Jaccard coefficient (JC) and the biotypes grouped by the UPGMA method. The results demonstrated a high dissimilarity (JC = 7.14 to 82.62) of the analyzed material, with the biotypes forming five...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; SSR; Tall fleabane; Genetic variability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100218

MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
Provedor de dados: 87
Autores: SILVA,D.R.O.; AGOSTINETTO,D.; VARGAS,L.
ABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in electrophoresis. Biotypes were identified as Conyza bonariensis and microcephala varieties. In molecular analysis, among 25 primers RAPD, eight were reproducible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conyza bonariensis; Polymorphism; Glyphosate; PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433

Single Nucleotide Polymorphisms in IGFBP-2 Gene and Their Associations with Body Weight Traits on Jinghai Yellow Chicken
Provedor de dados: 89
Autores: Zhao,XH; Li,MY; Xu,SS; Liu,GJ.
ABSTRACT Insulin-like growth factor binding protein-2 (IGFBP-2) regulates a broad spectrum of biological activities involved in growth, development, and differentiation. This study aimed at comparing polymorphisms in intron2 of the IGFBP-2 gene among four chicken breeds and at analyzing the associations between its genotypes and body weight in Jinghai Yellow chicken by using PCR-SSCP technique. For primer P2, three genotypes (AA, AB and BB) were observed in the four chicken breeds. Gene sequencing revealed one insertion/deletion (the inserted/deleted TC after position 552bp) in the intron 2 of IGFBP-2 gene. For primer P5, three genotypes were identified in Jinghai Yellow chickens, and named CC, CD and DD. Gene sequencing revealed two SNPs (C1107G, C1130T)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jinghai Yellow chicken; IGFBP-2 gene; PCR-SSCP; Polymorphism; Body weight.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400497

Microsatellite Analysis of Genetic Diversity in Quail Populations from China
Provedor de dados: 89
Autores: Bai,JY; Pang,YZ; Zhang,XH; Yun,YX; Qi,YX.
ABSTRACT Polymorphism of three quail communities was analyzed by using 12 microsatellite markers in this paper, aiming to provide scientific references for the evaluation, protection and utilization of quail genetic resources in China. Results demonstrated that the number of observed alleles by 12 microsatellite markers ranges between 4~7. The average polymorphism information contents (PIC) of the Chinese yellow quail, the Chinese black quail and the Korean quail, as detected by 12 microsatellite markers, are 0.6853, 0.6401 and 0.6565,respectively, and average heterozygosity values are 0.7333, 0.6957 and 0.7111, respectively. This indicates that the Chinese yellow quail has the richest genetic polymorphism. According to cluster analysis, the Chinese black...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chinese black quail; Microsatellite marker; Polymorphism; Genetic diversity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300519

Association Analysis Between Polymorphism of Gonadotrophin Releasing Hormone Genes and Growth Traits of Quail (Coturnix Coturnix)
Provedor de dados: 89
Autores: Bai,J; Dong,ZH; Lei,Y; Yang,YB; Jia,XP; Li,JY.
ABSTRACT This study investigated SNP mutation sites of Gonadotrophin releasing hormone (GnRH) gene in China yellow quail, Beijing white quail and Korean quail through PCR amplification and DNA sequencing technologies. Moreover, polymorphism of GnRH gene and its association with growth traits of quail were analyzed, aiming to get molecular markers associated to growth traits of quail, which could provide references for breeding of new quail species. According to research results, a total of 14 SNP mutation sites of GnRH were detected in China yellow quail, Beijing white quail and Korean quail, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T and C458T. Except that only 2 genotypes were detected for A209C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonadotrophin releasing hormone (GnRH) gene; DNA sequencing; SNP; Polymorphism; Body weight.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100312

A Novel Polymorphism of VLDLR Signal Peptide Coding Region and Its Association with Growth and Abdominal Fat Traits of Gaoyou Domestic Ducks
Provedor de dados: 89
Autores: Ming-liang,C; Cong,W; Xuan,D; Lu,L; Tang-jie,Z.
ABSTRACT The VLDLR gene plays important roles in the growth and adiposity in humans and mice. The purpose of this study was to investigate the relationship between VLDLR gene genetic polymorphisms and growth and abdominal fat traits of the Gaoyou domestic duck. A total of 267 Gaoyou ducks were employed for testing. A 18bp deletion was identified in VLDLR signal peptide coding region. The results of c2 test suggested that the genotype frequencies of VLDLR signal peptide coding region were not in Hardy-Weinberg equilibrium. Least squares analysis showed that body weight (BW) of -18bp/-18bp genotype ducks was significantly higher than those of other genotypes from six (BW6) (p&lt;0.05) to ten weeks of age (BW10) (p&lt;0.01). The association analysis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; VLDLR; Abdominal fat trait; Growth; Duck.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400577

Characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) varieties with five isozyme systems
Provedor de dados: 94
Autores: Corts,Remedios Morales; Rodrigues,Luciano Cordeiro; Marcide,Jesús Maria Ortíz; Sánches,Rodrigo Pérez.
Extracts from young leaves of nine sweet cherry (Prunus avium L.) and eight sour cherry (Prunus cerasus L.) varieties, located in the germplasm collection of the 'Direção Regional de Agricultura da Beira Interior' (Fundão, Portugal), were analysed for five isozyme systems in order to characterise these varieties and detect problems of synonymies and homonymies that frequently present. The sweet and sour cherry varieties analyzed showed low isoenzymatic polymorphism, being PGM and PGI the systems with the highest discrimination power. These systems presented seven and five different zymogrames, respectively. IDH showed four patterns. SKDH and 6-PGD grouped the varieties only into two patterns. The evident and discriminant restrictions of this type of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrogram; Polymorphism; Starch gel electrophoresis; Zymogram.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000100028

Paecilaema batman, a new species of Brazilian troglophilous harvestman that exhibits a remarkable color patches variation (Opiliones: Cosmetidae)
Provedor de dados: 103
Autores: Pinto-da-Rocha,Ricardo; Yamaguti,Humberto.
A new species of harvestman, Paecilaema batman, from Brazilian limestone caves of the state of Goiás, is described, and a remarkable intraspecific color patch variation is discussed. Paecilaema batman sp. nov. differs from other species of the genus by the following combination of features: chelicera similar in both sexes; prosoma without color patches; typical color patches on area I; and area III with two high spines. The new species is considered troglophilous.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intraspecific variation; Neotropics; Opilionids; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702013000400011

Paternity in Brazilian goats through the use of DNA microsatellites
Provedor de dados: 96
Autores: Araújo,Adriana Mello de; Guimarães,Simone Eliza Facioni; Pereira,Carmen Silva; Lopes,Paulo Sávio; Rodrigues,Marcelo Teixeira; Machado,Théa Mírian Medeiros.
A total of 292 animals from three breeds (Alpine and Saanen dairy breeds, and the Brazilian naturalized breed Moxotó) were genotyped, comprising 276 paternity cases. Statistical analyses were carried out by using TFPGA and CERVUS programs. Heterozygosis ranged from 0.542 (ILSTS005) to 0.825 (INRA006), with an average of 0.717 for all loci. Polymorphic information content (PIC) was 0.676 and 0.542, and combined exclusion probabilities (EP) were 0.999591 and 0.988375 for known and unknown maternal genotypes, respectively. The microsatellite system reveals 10% of paternity misidentification in evaluated registers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Local breed; Paternity exclusion; Pedigree mismatching; Polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500010

Effect of STAT1, OLR1, CSN1S1, CSN1S2 , and DGAT1 genes on milk yield and composition traits of Holstein breed
Provedor de dados: 96
Autores: Ardicli,Sena; Soyudal,Bahadir; Samli,Hale; Dincel,Deniz; Balci,Faruk.
ABSTRACT The objectives of the study were to evaluate allelic frequencies and test the association between STAT1, OLR1, CSN1S1, CSN1S2 , and DGAT1 gene polymorphisms and milk production traits including lactation milk yield, 305 days milk yield, days before peak milk production, and peak milk yield. In addition, samples of milk were analysed for fat, protein, lactose, and total solid contents. A total of 168 purebred Holstein-Fresian cows were genotyped using polymerase chain reaction and restriction fragment length polymorphism methods. Statistical analysis was carried out using least square methods of the general linear model procedure. Significant differences were found between genotypes of the CSN1S2 locus in relation to milk protein content. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Dairy cow; Milk composition; Polymorphism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100411

Dissecting the Opaque-2 regulatory network using transcriptome and proteome approaches along with enzyme activity measurements
Provedor de dados: 63
Autores: Lefèvre,Agnès; Consoli,Luciano; Gaziola,Salete A.; Pellegrino,Ana Paula; Azevedo,Ricardo A.; Damerval,Catherine.
The Opaque-2 (O2) gene encodes a transcriptional activator specifically expressed for grain development of maize. o2 mutants have an opaque and chalky kernel, with a decrease in zein storage protein content, and an increase in the proportions of lysine and tryptophan. In this review, we present recent results investigating genetic properties of the O2 network, using transcriptome and proteome approaches, associated with measurements of activities of enzymes of the aspartate pathway and lysine degradation. The structural polymorphism at the O2 locus was investigated by RFLP in a collection of 51 maize inbred lines. Most polymorphic sites were found outside the coding regions. We then searched for relationships between RFLP polymorphism and (i) mRNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zein; Aspartate kinase; Lysine oxoglutarate reductase/saccharopine dehydrogenase; Polymorphism.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200031

Genetic similarity of Brazilian hull-less and malting barley varieties evaluated by RAPD markers
Provedor de dados: 63
Autores: Kroth,Mariela Aparecida; Ramella,Micheline Sandra; Tagliari,Caroline; Francisco,Alicia de; Arisi,Ana Carolina Maisonnave.
Barley (Hordeum vulgare L.) is widely used for brewing and animal feed. Recently, it has become desirable for human consumption due to its high nutritional significance, specially hull-less or naked barley. There are differences in nutritional and malting characteristics among barley varieties. RAPD procedure is able to separate barley varieties at various similarity levels. The aim of this work was the RAPD analysis of six Brazilian hull-less varieties and seven malting varieties. PCR reactions were performed with eleven random primers. A total of 34 RAPD fragments was obtained with five primers. A dendrogram was constructed based on the Jaccard similarity coefficient. Barley varieties displayed an average similarity coefficient of 0.53. Intravarietal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hordeum vulgare; Polymorphism; Molecular characterization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100007

ISOENZYMATIC POLYMORPHISM AND ACTIVITY OF PEROXIDASES OF COMMON BEAN  (Phaseolus vulgaris L.) UNDER SALINE STRESS
Provedor de dados: 63
Autores: BROETTO,F.; CASA,A.M.; MALAVOLTA,E.; LOPES,C.R.
One of the uses of the technique of tissue culture for plant breeding is the identification of cell lines tolerant to salt stress.In order to study the biochemical mechanisms involved in the genetic expression to salt tolerance, callus from embryo axis of four bean cultivars (cv. IAC-carioca; cv. IAPAR-14; cv. JALO-EEP558; CV. BAT-93) were grown in Murashige &amp;amp; Skoog (1962) medium, supplemented with NaCl in the concentrations of 0, 20, 40, 60 and 80 mM. After 14 days callus were harvested and analyzed according to their isoenzymatic patterns and peroxidase activities. BAT and IAPAR cultivars showed two common activity zones in the anodic region, with only one specific enzymatic band to each one (the two fastest migration band); it is possible that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Peroxidases; Polymorphism; Salt stress; Phaseolus vulgaris.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161997000200003

Color polymorphism in Chauliognathus flavipes Fabricius (Coleoptera, Cantharidae): II. Patterns of emergence of morphs and mating system
Provedor de dados: 104
Autores: Machado,Vilmar; Araújo,Aldo Mellender de.
This study presents data on the color polymorphism in Chauliognathus flavipes Fabricius 1781 (Coleoptera, Cantharidae), analysing the variations in the emergence pattern of phenotypic classes for elytra color and mating system. The frequency of the phenotypic classes varies significantly throughout the season (males:X² = 59,705; females: X² = 61,017; 10 gl; p&lt;0.001) being similar in males and females. The frequency of classes does not differ significantly among the insects that were copulating and those that were not copulating at the time of collection, i.e., the matings are random for elytra color patterns. The variations observed in frequency of phenotypic classes are not influenced by factors associated with sex. Furthermore, the elytra color...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Phenotypic class; Emergence; Assortative mating; Coleoptera.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000200010

Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
Provedor de dados: 104
Autores: Aidar,Davi S.; Pagotto,Rubiane C.; Contel,Eucleia P.B.
Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona quadrifasciata anthidioides; Polymorphism; Electrophoresis; Enzyme; Stingless bees.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018

GENETIC VARIABILITY OF THE ZEBU CATTLE BREED (Bos indicus) IN THE DEPARTAMENT OF HUILA, COLOMBIA USING MICROSATELLITE MOLECULAR MARKERS
Provedor de dados: 37
Autores: HERNÁNDEZ ESCOBAR,CARLOS; OLIVERA ÁNGEL,MARTHA; OSTOS ALFONSO,HENRY; GUERRA,MARIA TERESA.
The polymorphism of 11 microsatellites from zebu cattle (Bos indicus) was studied using a commercial multiplex system to estimate genetic variability. Allele frequencies polymorphism information content and heterozygosis were calculated. Allele frequencies revealed that in the analyzed sample the markers were not equally polymorphic. The average allele was 14.2 with the highest values for the TGLA122 microsatellites. The mean heterozygocity was 0.7056 and the polymorphism information content was 0.668. This multiplex analysis could be used for pedigree information and for adequate genetic improvements in breeding programs and paternity test.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alleles; Microsatellite; Polymorphism; Zebu.
Ano: 2009
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2009000300013

Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases
Provedor de dados: 39
Autores: Pati,Prakash G; Datta,Subhojit; Agbagwa,Ikechukwu O; Singh,Indra Prakash; Soren,Khela Ram; Das,Alok; Choudhary,Arbind Kumar; Chaturvedi,Sushil Kumar.
Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP-RGA; Cajanus cajan; Genetic diversity; Molecular markers; Polymorphism.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007

Cross-amplification and characterization of microsatellite markers in species of Manihot Mill. (Euphorbiaceae) endemic to the Brazilian Cerrado
Provedor de dados: 39
Autores: Miranda,Kássia Marques Corrêa; Guimarães,Rejane Araújo; Silva,Marcos José da; Oliveira,Patrícia Rasteiro Ordiale; Ribeiro,Thania Gonçalves; Mendes,Thainara Policarpo; Telles,Mariana Pires de Campos; Soares,Thannya Nascimento.
ABSTRACT The genus Manihot Mill. contains about 120 species of which about 104 occur in Brazil. We tested the cross-amplification of ten microsatellite markers developed for Manihot esculenta in 15 species of Manihot endemic to the Brazilian Cerrado. We also evaluated the genetic diversity of Manihot irwinii, M. orbicularis, and M. purpureocostata. Ten pairs of primers were amplified among 14 species of Manihot. The percentage of polymorphic loci per species varied from 70 to 100 %. Nine markers showed amplification and polymorphism when evaluated on polyacrylamide gel. The markers were combined to form three sets for multiplex genotyping for genetic diversity analysis, and showed 51, 75, and 75 alleles in M. irwinii, M. orbicularis, and M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Polymorphism; SSR; Transferability wild cassava.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400772

Polymorphism in the desmid Cosmarium abbreviatum var. minus (Zygnemaphyceae) and its taxonomic implications
Provedor de dados: 39
Autores: Bicudo,Carlos E. de M.
Polymorphism in a population of the desmid (Zygnemaphyceae) Cosmarium abbreviatum Raciborski var. minus (West &amp;amp; West) Krieger &amp;amp; Gerloff is reported from material collected from 3 stations at the Lily Pond located in the Parque Estadual das Fontes do Ipiranga, São Paulo City, southern Brazil. From the examination of approximately 200 plants, 6 different morphological expressions were described and the following remarks made: 1) a detailed analysis of sample populations is absolutely necessary for the definition of species and infraspecific categories among desmids; 2) the morphological characteristics presently used for the delimitation of certain species, which are built up of individuals of very small size, seem to have no great...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmarium abbreviatum var. minus; Polymorphism; Synonymy; Zygnemaphyceae; Cosmarium abbreviatum var. minus; Desmídia; Polimorfismo; Sinonimia; Zygnemaphyceae.
Ano: 1988
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33061988000100001

Population genetics of the sugarcane borer Diatraea saccharalis (Fabr.) (Lepidoptera: Crambidae)
Provedor de dados: 1
Autores: Lopes, Denise Alves; Universidade Estadual de Maringá; Cantagalli, Liriana Belizário; Universidade Estadual de Maringá; Stuchi, Ana Lucia Paz Barateiro; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Ruvolo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá.
Diatraea saccharalis is the principal pest of sugarcane in Brazil and is found throughout the sugarcane crop. Information about its population genetics is scarce, but population genetic analysis is of particular importance as a basis for a successful pest control program. Pest control requires a constant evaluation of genetic variability so that appropriate strategies can be employed. In this study, the structure of D. saccharalis populations in sugarcane crops was analyzed with PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) markers. Samples were collected from four sugarcane fields in ParanáState and one in São PauloState. Twelve PCR-RAPD primers were used. The analysis produced 216 fragments, with a 99.54% polymorphism rate. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.02.02-8 and integrated using chemical and biological control.  molecular marker; Polymorphism; PCR-RAPD; Sugarcane pest Fitossanidade.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16211

Genetic variability in Oligosarcus paranensis (Teleostei: Characiformes) from the São Francisco river, Ivaí river basin – Paraná State, Brazil - doi: 10.4025/actascibiolsci.v35i3.14179
Provedor de dados: 3
Autores: Santos, Michele Rocha dos; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá.
The genetic variability of Oligosarcus paranensis was estimated from a population collected in São Francisco river, Prudentópolis county in Paraná State (Brazil) using the electrophoresis in starch gel technique. Eleven enzymatic systems were analyzed: Aspartate aminotransaminase (AAT; E. C. 2.6.1), Alcohol dehydrogenase (ADH; E. C. 1.1.1.1), Esterase (EST; E. C. 3.1.1.1), Glucose-6-phosphate isomerase (GPI; E. C. 5.3.1.9), Glycerol-3-Phosphate dehydrogenase (G3PDH; E. C. 1.1.1), Isocitrate dehydrogenase (IDH; E. C. 1.1.1.42), L-lactate dehydrogenase (LDH; E. C. 1.1.1.27), Malate dehydrogenase (MDH; E. C. 1.1.1.37 ), Malate dehydrogenase NADP (ME; E. C. 1.1.1.40), Phosphoglucomutase (PGM; E. C. 5.4.2.2) and Sorbitol dehydrogenase (SORB; E.C. 1.1.1.14)....
Palavras-chave: Allozyme; Genetic diversity; Heterozygosity; Polymorphism; Pisces.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14179

Utilization of microsatellite markers to form families of "pejerrey" Odontesthes bonariensis in a genetic breeding program
Provedor de dados: 45
Autores: Tavares,R.A; Nunes,M.D; Almeida,D.B; Silva,J.C; Vaz,B.S; Moreira,C.G.A; Dionello,N.J.L; Piedras,S.R.N; Moreira,H.L.M.
Foram identificadas a divergência e a variabilidade genética, por meio do polimorfismo de seis marcadores microssatélites, de duas populações de Odontesthes bonariensis, utilizadas em manejos de cultivo e com potencial para fornecimento de reprodutores para programas de melhoramento genético. Do total de seis loci, cinco demonstraram eficiência para análise genética nas duas populações de O. bonariensis. A diferenciação genética significante nas populações analisadas pode fornecer a base para futuros programas de melhoramentos genéticos, através da combinação de material das populações divergentes para o desenvolvimento de linhagens ou execução de um programa de seleção.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Genetic variability; Polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500034

Assessment of changes in the brca2 and p53 genes in breast invasive ductal carcinoma in northeast Brazil
Provedor de dados: 48
Autores: Ramalho,Eduardo AVF; Silva-Filho,João LQ; Cartaxo,Marina FS; Cavalcanti,Carmelita BL; Rêgo,Moacyr JBM; Oliveira,Maria BM; Beltrão,Eduardo IC.
BACKGROUND: BRCA protein interacts with at least 13 different proteins that have been implicated with cancer susceptibility and loss of BRCA function is correlated to sensitivity to DNA crosslinking agents in preclinical models. RESULTS: BRCA2 methylation frequency was 44%, p53 Pro22 allele frequency was 32% and heterozygous frequency of Arg/Pro72 genotype was 60% which could be associated as risk factor for metastasis (p = 0.046 OR = 4.190). Regarding to polymorphism of codon 249 the frequency of Arg249 allele presented 82% which was considered not statistically significant. CONCLUSIONS: There was not statistical significance to BRCA2 promoter methylation with any parameters chosen. However, our findings suggest that patients who present heterozygous...
Tipo: Journal article
Palavras-chave: Breast cancer; Epigenetics; Metastasis; Methylation; Polymorphism.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100003

Analysis of the intronic single nucleotide polymorphism rs#466452 of the nephrin gene in patients with diabetic nephropathy
Provedor de dados: 48
Autores: GONZÁLEZ,RODRIGO; TIRADO,ARES; ROJAS,LUIS A; OSSANDÓN,FRANCISCO J; ALVO,MIRIAM; WOLFF,CARLOS; SEELENFREUND,DANIELA; DURRUTY,PILAR; LOBOS,SERGIO.
We present the analysis of an intronic polymorphism of the nephrin gene and its relationship to the development of diabetic nephropathy in a study of diabetes type 1 and type 2 patients. The frequency of the single nucleotide polymorphism rs#466452 in the nephrin gene was determined in 231 patients and control subjects. The C/T status of the polymorphism was assessed using restriction enzyme digestions and the nephrin transcript from a kidney biopsy was examined. Association between the polymorphism and clinical parameters was evaluated using multivaríate correspondence analysis. A bioinformatics analysis of the single nucleotide polymorphism rs#466452 suggested the appearance of a splicing enhancer sequence in intron 24 of the nephrin gene and a...
Tipo: Journal article
Palavras-chave: Diabetic nephropathy; NPHS1; Polymorphism; Splicing enhancer.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200007

Molecular analysis of the eighteen most frequent mutations in the BRCA1 gene in 63 Chilean breast cancer families
Provedor de dados: 48
Autores: JARA,LILIAN; AMPUERO,SANDRA; SANTIBÁÑEZ,EUDOCIA; SECCIA,LORENA; RODRÍGUEZ,JUAN; BUSTAMANTE,MARIO; LAY-SON,GUILLERMO; OJEDA,JOSÉ MANUEL; REYES,JOSÉ MIGUEL; BLANCO,RAFAEL.
BRCA1 gene mutations account for nearly all families with multiple cases of both early onset breast and/or ovarian cancer and about 30% of hereditary breast cancer. Although to date more than 1,237 distinct mutations, polymorphisms, and variants have been described, several mutations have been found to be recurrent in this gene. We have analyzed 63 Chilean breast/ovarian cancer families for eighteen frequent BRCA1 mutations. The analysis of the five exons and two introns in which these mutations are located was made using mismatch PCR assay, ASO hybridization assay, restriction fragment analysis, allele specific PCR assay and direct sequentiation techniques. Two BRCA1 mutations (185delAG and C61G) and one variant of unknown significance (E1250K) were found...
Tipo: Journal article
Palavras-chave: BRCA1 gene; Frequent mutations; Polymorphism; 4185delCAAG; New mutation; Chilean population.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300011

Foundational errors in the Neutral and Nearly-Neutral theories of evolution in relation to the Synthetic Theory: Is a new evolutionary paradigm necessary?
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
The Neutral Theory of Evolution (NTE) proposes mutation and random genetic drift as the most important evolutionary factors. The most conspicuous feature of evolution is the genomic stability during paleontological eras and lack of variation among taxa; 98% or more of nucleotide sites are monomorphic within a species. NTE explains this homology by random fixation of neutral bases and negative selection (purifying selection) that does not contribute either to evolution or polymorphisms. Purifying selection is insufficient to account for this evolutionary feature and the Nearly-Neutral Theory of Evolution (N-NTE) included negative selection with coefficients as low as mutation rate. These NTE and N-NTE propositions are thermodynamically (tendency to random...
Tipo: Journal article
Palavras-chave: Fixation; Loss; Neutral evolution; Polymorphism; Randomness; Selectiveness.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200001

DNA Repair Genes XRCC1, XRCC3, XPD, and OGG1 Polymorphisms among the Central Region Population of Saudi Arabia
Provedor de dados: 48
Autores: Alanazi,Mohammad; Pathan,Akbar Ali Khan; Ajaj,Sana Abdulla; Khan,Wajahatullah; Shaik,Jilani P; Al Tassan,Nada; Parine,Narasimha Reddy.
DNA repair is one of the central defense mechanisms against mutagenic exposures. Inherited SNPs of DNA repair genes may contribute to variations in DNA repair capacity and susceptibility to cancer. Due to the presence of these variants, inter-individual and ethnic differences in DNA repair capacity have been established in various populations. Saudi Arabia harbors enormous genetic and cultural diversity. In the present study we aimed to determine the genotype and allele frequencies of XRCC1 Arg399Gln (rs25487), XRCC3 Thr241Met (rs861539), XPD Lys751Gln (rs13181), and OGG1 Ser326Cys (rs1052133) gene polymorphisms in 386 healthy individuals residing in the central region of Saudi Arabia and compare them with HapMap and other populations. The genotype and...
Tipo: Journal article
Palavras-chave: DNA Repair genes; Polymorphism; Saudi population; XRCC1; XRCC3; OGG1; XPD.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200007

lnterleukin-8 gene polymorphism and susceptibility to gastric cancer in a brazilian population
Provedor de dados: 48
Autores: Felipe,Aledson V; Silva,Tiago D; Pimenta,Célia A; Kassab,Paulo; Forones,Nora M.
BACKGROUND: Studies have demonstrated that some polymorphisms in different interleukin genes may increase the risk of cancer. The aim of this study was to investigate the association between the IL-8 (rs4073) -251A/T gene polymorphism and the risk of gastric cancer (GC). PATIENTS AND METHODS: A case-control study was conducted on patients with noncardia gastric cancer. DNA was extracted from leukocytes and the IL-8 (rs4073) -251A/T polymorphism was analyzed by PCR-RFLP. Infection with Helicobacter pylori was investigated in the serum by ELISA. RESULTS: The sample consisted of 104 patients with GC and 196 controls. Cigarette smoking (P=0.007) and high fat intake (P=0.01) were more frequent in patients with GC. The proportion of patients infected with H....
Tipo: Journal article
Palavras-chave: IL-8; Polymorphism; Gastric cancer.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400007

Association of TNF promoter polymorphisms with type 1 diabetes in the South Croatian population
Provedor de dados: 48
Autores: BORASKA,VESNA; ŠKRABI,VESELIN; IKEŠ ULI,VEDRANA; BEI,KRISTIJAN; KAPITANOVI,SANJA; ZEMUNIK,TATIJANA.
Type 1 diabetes mellitus (TIDM) is an autoimmune disease characterized by the destruction of pancreatic p cells. Tumor necrosis factor (TNF) is a pleotropic cytokine with potent immunomodulatory and inflammatory activity. Association studies of TNF polymorphisms and type 1 diabetes (TIDM) frequently demonstrated TNF involvement with TIDM. Although TNF may play an important role in the pathogenesis of TIDM, the genetic association of TNF región with the disease has not been conclusive because of the strong linkage disequilibrium with HLA genes. In this study, we examined two TNF promoter variants (rs 1800629 at position -308, and rs361525 at position -238) for TIDM association in 233 patients and 144 controls from the population of South Croatia. A higher...
Tipo: Journal article
Palavras-chave: Type 1 diabetes; Tumor necrosis factor gene (TNF); Polymorphism; Genetic epidemiology; Croatia.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200005

A pilot study on genetic variation in purine-rich elements in the nephrin gene promoter in type 2 diabetic patients
Provedor de dados: 48
Autores: GONZÁLEZ,RODRIGO; TIRADO,ARES; BALANDA,MONSERRAT; ALVO,MIRIAM; BARQUÍN,INÉS; DURRUTY,PILAR; LOBOS,SERGIO; SEELENFREUND,DANIELA.
Diabetic nephropathy (DN) is one of the major complications of type 2 diabetes and is associated with coronary disease. Nephrin, a protein mainly expressed in glomeruli, is decreased in DN and other kidney diseases. Since insulin levels are misregulated in type 2 diabetes, a possible connection between DN and its decreased nephrin expression could be the presence of regulatory elements responsive to insulin in the nephrin gene (NPHS1) promoter region. In this work, using bioinformatic tools, we identified a purine-rich GAGA element in the nephrin gene promoter and conducted a genomic study in search of the presence of polymorphisms in this element and its possible association with DN in type 2 diabetic patients. We amplified and sequenced a 514 bp promoter...
Tipo: Journal article
Palavras-chave: GAGA box; Gene promoter; Nephrin; Polymorphism.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400010

Association between polymorphism of the G-protein &amp;#946;3 subunit C825T and essential hypertension: an updated meta-analysis involving 36,802 subjects
Provedor de dados: 48
Autores: Zheng,Huan; Xu,Huifeng; Cui,Bin; Xie,Nanzi; Wang,Zhi; Luo,Ming.
Purpose: The G-protein &amp;#946;3-subunit gene C825T polymorphism (GNB3-C825T) has been reported to be associated with essential hypertension (EH), but results from previous studies are conflicting. The present study aimed at investigating the association between this polymorphism and risk of EH using a meta-analysis on the published studies. Materials and Methods: PubMed, Embase, CBM (China Biological Medicine Database), Wanfang and VIP databases were searched to identify eligible studies published in English and Chinese before March 2013. Data were extracted using standardized methods. The association was assessed by the odds ratio (OR) with 95% confidence intervals (CI). Begg's test was used to measure publication bias. Results: A total of 40...
Tipo: Journal article
Palavras-chave: GTP-Binding Proteins; Polymorphism; Genetic hypertension; Meta-Analysis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300007

Occurrence of concavities on the lorica of two species of Testudinella (Rotifera, Monogononta, Testudinellidae)
Provedor de dados: 49
Autores: Coelho,Paula Nunes; Braghin,Louizi Souza Magalhães; Lansac-Tôha,Fabio Amodêo; Henry,Raoul.
Abstract: Testudinella is a taxon of Rotifera broadly distributed in Brazil. In a recent collection in marginal lakes connected to the Paranapanema River (São Paulo, Brazil), we found specimens of Testudinella mucronata and T. patina with concavities on their lorica. The objective of this study was to describe the concavities observed in individuals of both species and discuss their possible causes. Plankton samples were collected at two sampling stations, in the pelagic (PZ) and littoral (LZ) zones of the Panema and Coqueiral lakes; both of these lakes are ultra-oligotrophic and not polluted. For T. mucronata, 4% of individuals with concavities were found in the PZ of Coqueiral lake and 50% in the LZ of Panema lake. In T. patina, the proportion of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater environments; Marginal lakes; Morphology; Rotifers; Polymorphism.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000200205

Checklist and description of three new chromatic patterns of Pachycoris torridus (Scopoli, 1772) (Hemiptera: Scutelleridae)
Provedor de dados: 49
Autores: Souza-Firmino,Tatiani Seni de; Alevi,Kaio Cesar Chaboli; Pereira,Luis Lenin Vicente; Banho,Cecilia Artico; Silva Junior,Fernando Cesar; Souza,Emi Rosane Silistino de; Itoyama,Mary Massumi.
In the present paper, 27 chromatic patterns of the specie Pachycoris torridus (Scopoli, 1772) were grouped and three new patterns are described. Because of this high phenotypic polymorphism, P. torridus already been registered eight times as a new specie, highlighting the importance of the application of different tools to assist in taxonomy of this hemipterous of economic importance.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Insect; Heteroptera; Polymorphism; Agricultural pest.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032016000100406

Amplification of microsatellites in two haemulid species (Haemulon aurolineatum and Haemulon steindachneri)
Provedor de dados: 50
Autores: Pabón Quintero,Paula E.; Tavera,José Julián; Millán-Márquez,Ana María; Acero P,Arturo.
ABSTRACT Genetic-population studies in marine fish have allowed to study patterns of dispersal and connectivity between habitats. One important tool in population genetics is the use of microsatellite molecular markers. Cross-amplification of microsatellite is a method that consists in using primers designed for one species in a different one but phylogenetically related. Because of the importance of genetic studies of populations in artisanal fisheries species, primers were evaluated and designed for the species Haemulon aurolineatum and Haemulon steindachneri. Samples were collected from the artisanal fisheries in Baru-Colombia. Amplification was standardized for 12 microsatellites which ten were polymorphic for H. aurolineatum and nine for H....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemulidae; Grunts; Primers; Polymorphism; Population genetics.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612018000100151

Genetic diversity in a natural population of the halophytic legume Prosopis strombulifera revealed by AFLP fingerprinting
Provedor de dados: 51
Autores: Llanes,Analia; Bonercarrere,Victoria; Capdevielle,Fabian; Vidal,Sabina; Luna,Virginia.
Prosopis strombulifera (Lam.) Benth. is a spiny shrub with the maximum tolerance limits reported for halophytic plants. This species is frequently found in the salinized areas in south-western of Córdoba and San Luis provinces, Argentina. Little is known about the genetic diversity within this species in a native population. Genetic diversity in 60 plants of P. strombulifera in south-western San Luis was investigated using AFLP analysis. Polymorphism was found among the samples tested. Four combinations of primers led to the identification of an average of 250 polymorphic bands and the data were used for cluster analysis. P. strombulifera genotypes are clearly separated in subclusters and reflect the diversity within the collection area. This study is a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prosopis strombulifera; AFLP; Genetic diversity; Polymorphism.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722011000200010

Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers
Provedor de dados: 52
Autores: Souza,Silvia Graciele Hülse de; Carpentieri-Pípolo,Valéria; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Ruas,Paulo Maurício; Gerage,Antônio Carlos.
The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn; Distance genetic; Microsatellites; Molecular markers; Polymorphism; RAPD.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100022

Prevalence of estrogen receptor alpha PvuII (c454-397T&gt;C) and XbaI (c454A&gt;G) polymorphisms in a population of Brazilian women
Provedor de dados: 52
Autores: Araújo,Karine Lourenzone; Rezende,Lucas Cunha Dias de; Souza,Letícia Soncini; Daltoé,Renata Dalmaschio; Madeira,Klesia Pirola; Paes,Marcela Ferreira; Herkenhoff,Fernando Luiz; Rangel,Letícia Batista Azevedo; Silva,Ian Victor.
The aim of this work was to study the estrogen receptor alpha (ERS1) PvuII and XbaI gene polymorphisms prevalence in randomly selected women population from Rio de Janeiro and Espírito Santo states in Brazil by polymerase chain reaction restriction fragment lengths polymorphism (PCR_RFLP) methodology. It was shown that Rio de Janeiro women exhibited a significantly different prevalence of XbaI polymorphism comparing to Espirito Santo women. Nonetheless, similar prevalence of PvuII polymorphism was found in both women´s populations. Moreover, a strong linkage disequilibrium was observed between these SNPs reinforcing the hypothesis of differential pattern of inheritance observed on such populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Estrogen Receptor; Brazilian Women; Prevalence; Linkage disequilibrium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600010

Genetic variability of brycon orbignyanus (valenciennes, 1850) (characiformes: characidae) in cultivated and natural populations of the upper paraná river, and implications for the conservation of the species
Provedor de dados: 52
Autores: Panarari-Antunes,Renata de Souza; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Galdino,Alexsandro Sobreira; Julio Junior,Horácio Ferreira; Prioli,Laudenir Maria.
This study aimed to evaluate the genetic variability of B. orbignyanus in cultivated and natural populations of the Upper Paraná River, using molecular RAPD markers and mtDNA control region. Specimens were collected in the Paraná River and in the Piracema fish farm in Maringá, State of Paraná, Brazil. RAPD primers produced 82 loci with consistent expression. The population from the Paraná River showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. Data revealed genetic differentiation between the two populations, although not very pronounced. These results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. The alignment of the D-loop sequences of B. orbignyanus indicated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Endangered; RAPD; MtDNA; Polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400025

Efficiency of RAPD and ISSR markers in assessing genetic variation in Arthrocnemum macrostachyum (Chenopodiaceae)
Provedor de dados: 52
Autores: Saleh,Basel.
The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002

Dissolution profile evaluation of solid pharmaceutical forms containing chloramphenicol marketed in Brazil
Provedor de dados: 52
Autores: Ferraz,Humberto Gomes; Carpentieri,Leticia Norma; Watanabe,Sayuri Pereira.
The dissolution profile for solid pharmaceutical forms containing chloramphenicol 250 mg available in Brazil was determined using a method from the American Pharmacopoeia (United States Pharmacopoeia, 2004) and then compared. Two different methods of dissolution profile comparison were used: ANOVA, and an independent model. Differences between the formulations were reflected in the dissolution profiles. The presence of metastable polymorphs or amorphous forms of chloramphenicol palmitate might be responsible for variations in the concentration of the drug observed within formulations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloramphenicol; Dissolution; Polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100007

Transferrin polymorphism in Amazon turtle (Podocnemis expansa) stocks
Provedor de dados: 54
Autores: Teixeira,Aylton Saturnino; Jamieson,Alan; Raposo,José Carlos Paula; Vieira,Alvaro Alves.
The transferrin gene locus (Tf) was investigated in five populations of the Amazon turtle (Podocnemis expansa) sampled from five geographical areas in the Amazon region. This locus was polymorphic, showing three genotypes (Tfª Tfª, Tfª Tf b and Tf b Tf b), presumably encoded by two co-dominant alleles, Tfª and Tf b. All populations showed good genetic balance according to Hardy-Weinberg expectations, and may sustain the hypothesis of a single stock in the area investigated. The data are consistent with free flow of genes among the population samples examined.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Amazon turtle.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400004

TNF -308G &gt; a promoter polymorphism (rs1800629) and outcome from critical illness
Provedor de dados: 55
Autores: Paskulin,Diego D'Ávila; Fallavena,Paulo RV; Paludo,Francis JO; Borges,Thiago J; Picanço,Juliane B; Dias,Fernando S; Alho,Clarice Sampaio.
BACKGROUND: The susceptibility to adverse outcome from critical illness (occurrence of sepsis, septic shock, organ dysfunction/failure, and mortality) varies dramatically due to different degrees of inflammatory response. An over expression of tumor necrosis factor alpha (TNF-α) can lead to the progression of the inflammatory condition. OBJECTIVE: We assessed the relationship of the genotype distribution of -308G &gt;A TNF-α polymorphism with regard to the development of sepsis, septic shock, higher organ dysfunction or mortality in critically ill patients. METHODS: Observational, hospital-based cohort study of 520 critically ill Caucasian patients from southern Brazil admitted to the general ICU of São Lucas Hospital, Porto Alegre, Brazil. Patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Single nucleotide tumor necrosis factor receptor associated peptides and proteins critical care.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300009

Genetic polymorphism and immune response to tuberculosis in indigenous populations: a brief review
Provedor de dados: 55
Autores: Longhi,Renata Maronna Praça; Zembrzuski,Verônica Marques; Basta,Paulo Cesar; Croda,Julio.
We systematically reviewed studies of the immune response to tuberculosis and the genetic polymorphisms associated with Th1-or Th2-mediated cytokine expression in indigenous populations. A bibliographic search was performed on the Medline and ISI databases and included studies published between January 1980 and October 2011. The search terms were tuberculosis, American Indians, Amerindian, indigenous, Indians, native people, aboriginal, immun*, host immune, immune response, cytokine*, polymorphism*, and gene. Regardless of their design, studies that evaluated immunoglobulin, cytokine levels and genetic polymorphisms that altered cytokine expression were included. Thirteen studies met the inclusion criteria. The majority of studies were performed in Latin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Immune response; Indigenous populations; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300009

Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFlP and DNA sequencing methods
Provedor de dados: 55
Autores: Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva.
Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009

HLA-Bw4-B*57 and Cw*18 alleles are associated with plasma viral load modulation in HIV-1 infected individuals in Salvador, Brazil
Provedor de dados: 55
Autores: Silva,Edinete Melo da; Acosta,Angelina Xavier; Santos,Eduardo José Melo; Netto,Eduardo Martins; Lemaire,Denise Carneiro; Oliveira,Adriano Silva; Barbosa,Carolina Matos; Bendicho,Maria Teresita; Galvão-Castro,Bernardo; Brites,Carlos.
Host genetic factors play an important role in mediating resistance to HIV-1 infection and may modify the course of infection. HLA-B alleles (Bw4 epitope; B*27 and B*57) as well as killer cell immunoglobulin-like receptors have been associated with slow progression of HIV-1 infection. OBJECTIVE: To evaluate the association between serological epitopes HLA-Bw4 and HLA-Bw6 and prognostic markers in AIDS. METHODS: 147 HIV-infected individuals in Bahia, Northeast Brazil, were genotyped for HLA class I locus. HLA class I genotyping was performed by hybridization with sequence-specific oligonucleotide probes following amplification of the corresponding HLA-A, HLA-B and HLA-C genes. Statistical analysis was performed using Fisher's exact and ANOVA tests for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; AIDS; MHC; HLA; Polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500008

Helicobacter pylori eradication: influence of interleukin-1beta -31 C/T polymorphism
Provedor de dados: 55
Autores: Rech,Tássia Flores; Mazzoleni,Luiz Edmundo; Mazzoleni,Felipe; Francesconi,Carlos Fernando de Magalhães; Sander,Guilherme Becker; Michita,Rafael Tomoya; Nabinger,Débora Dreher; Milbradt,Tobias Cancian; Torresini,Ronaldo João Spinato; Simon,Daniel.
ABSTRACT  Aim To analyze the influence of the -31 C/T polymorphism of the interleukin-1β gene on Helicobacter pylori eradication therapy success in patients with functional dyspepsia.  Methods Functional dyspepsia was diagnosed according to the Rome III criteria. All patients underwent upper gastrointestinal endoscopy, and gastric biopsies were obtained at screening and 12 months after randomization (last follow-up visit). Urease test and histological examination were performed to define the H. pylori status. Patients received twice-daily amoxicillin, clarithromycin and omeprazole for 10 days. Genotyping of the interleukin-1beta -31 C/T polymorphism (rs1143627) was performed using polymerase chain reaction-restriction fragment length polymorphism.  Results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Dyspepsia; Interleukin-1beta; Polymorphism; Inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400311

Interleukin-10 gene polymorphism (-1082G/A) and allergy to efavirenz in patients infected with human immunodeficiency virus
Provedor de dados: 55
Autores: Rodrigues,Raphael de Oliveira; Carvalho,Paulo Germano de; Arruda,Érico Antônio Gomes de; Rabenhorst,Silvia Helena Barem; Silva,Silvia Fernandes Ribeiro da; Ribeiro,Ilana Farias; Lima,Denise Girão Limaverde; Nagao-Dias,Aparecida Tiemi.
The aim of the present study was to investigate the association between polymorphism in the interleukin-10 gene promoter at position -1082 in human immunodeficiency virus-infected patients who had presented allergic reaction due to efavirenz. The study included 63 patients treated at the Hospital São José de Doenças Infecciosas, Fortaleza, Ceará, Brazil. Twenty-one patients who had presented allergic reaction to efavirenz were compared to 42 patients with no allergic reaction following exposure to this drug. Blood samples were collected for DNA extraction and submitted to the restriction fragment length polymorphism - polymerase chain reaction technique. The -1082AA genotype was significantly more frequent in allergic patients as compared to non-allergic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV; Drug allergy; Polymorphism; IL-10.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400445

The association of HLA-DRB1 alleles and drug use with HIV infection in a Chinese Han Cohort
Provedor de dados: 55
Autores: Diao,Bo; Du,Juan; Liu,Ying; Luo,Fan; Hou,Wei.
Objective:To investigate the relationship between the polymorphism of human leukocyte antigen (HLA)-DRB1 and the susceptibility and repellency of drug use combined with HIV infection in Chinese.Methods:A total of 213 unrelated healthy people, 41 HIV-infected drug users, 24 HIV-uninfected drug users, and 64 HIV-infected non-drug users were recruited. Their HLA-DRB1 allele frequencies were analyzed by PCR-SSP and allele distribution was analyzed.Results:Compared with healthy controls, in drug users, the frequencies of HLA-DRB1 *0401-041, *1001 were significantly higher; in HIV-infected patients, the frequencies of HLA-DRB1 *0101-0103, *0401-0411, *1001 were significantly higher, while the frequencies of DRB1 *1501-1502, *1101-1105, *1301-1302, DRB4, DRB5...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: HLA-DRB1; Polymorphism; Drug use combined with HIV; Infection; Frequency.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100082

Association of genetic polymorphisms of CISH with the risk of pulmonary tuberculosis in Zahedan, Southeast Iran
Provedor de dados: 55
Autores: Naderi,Mohammad; Hashemi,Mohammad; Safdari,Abolhassan; Bahari,Gholamreza; Taheri,Mohsen.
Abstract  Background In the current study we aimed to find out the impact of cytokine-inducible Src homology 2 domain protein (CISH) gene polymorphisms on the risk of pulmonary tuberculosis (PTB) in a sample of Iranian population.  Materials and methods Polymorphisms of CISH rs2239751, rs414171, and rs6768300 were determined in 200 PTB patients and 200 healthy subjects using T-ARMS-PCR or PCR-RFLP method.  Results The results showed that rs414171 A&gt;T genotypes significantly decreased the risk of PTB (OR = 0.16, 95% CI = 0.10–0.27, p &lt; 0.0001, AT vs AA; OR = 0.31, 95% CI = 0.14–0.68, p &lt; 0.0001, TT vs AA; OR = 0.19, 95% CI = 0.12–0.29, p &lt; 0.0001, AT+TT vs AA; OR = 0.29, 95%CI = 0.20–0.42, p &lt; 0.0001, T vs A). For rs6768300, the findings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; CISH; Polymorphism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400379

Effect of estrogen receptor-alpha (ESR1) gene polymorphism on high density lipoprotein levels in response to hormone replacement therapy
Provedor de dados: 56
Autores: Nogueira-de-Souza,N.C.; Guerreiro da Silva,I.D.C.; de Carvalho,C.V.; Pulchinelli,A.; Haidar,M.A.; Baracat,E.C.; Massad-Costa,A.M.
Studies have shown that estrogen replacement therapy and estrogen plus progestin replacement therapy alter serum levels of total, LDL and HDL cholesterol levels. However, HDL cholesterol levels in women vary considerably in response to hormone replacement therapy (HRT). A significant portion of the variability of these levels has been attributed to genetic factors. Therefore, we investigated the influence of estrogen receptor-alpha (ESR1) gene polymorphisms on HDL levels in response to postmenopausal HRT. We performed a prospective cohort study on 54 postmenopausal women who had not used HRT before the study and had no significant general medical illness. HRT consisted of conjugated equine estrogen and medroxyprogesterone acetate continuously for 1 year....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hormone replacement therapy; High-density lipoprotein cholesterol levels; Estrogen receptor-alpha; Polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200003

Association study of AFF1 rs340630 polymorphism with genetic susceptibility to rheumatoid arthritis in Chinese population
Provedor de dados: 56
Autores: Sun,Qing-Qing; Hua,Dong-Jin; Huang,Si-Chao; Cen,Han; Zhou,Li; Shao,Song.
This study was performed to examine whether the AF4/FMR2 family, member 1 (AFF1) rs340630 polymorphism is involved in the genetic background of rheumatoid arthritis (RA) in a Chinese population. Two different study groups of RA patients and controls (328 RA patients and 449 healthy controls in the first study group; 232 RA patients and 313 controls in the second study group) were included in our study. Overall, there was no significant difference in either genotype (P=0.71 and 0.64 in the first and second study group, respectively) nor allele (in the first study group: A vs G, P=0.65, OR=1.05, 95%CI=0.85–1.29; in the second study group: G vs A, P=0.47, OR=1.10, 95%CI=0.86–1.40) frequencies of AFF1 rs340630 polymorphism between RA patients and controls. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; AFF1; Polymorphism; Chinese.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700606

CYP2A6 and CYP2E1 polymorphisms in a Brazilian population living in Rio de Janeiro
Provedor de dados: 56
Autores: Rossini,A.; Soares Lima,S.; Rapozo,D.C.M.; Faria,M.; Albano,R.M.; Ribeiro Pinto,L.F.
Cytochrome P450 (CYP) is a superfamily of enzymes involved in the metabolism of endogenous compounds and xenobiotics. CYP2A6 catalyzes the oxidation of nicotine and the activation of carcinogens such as aflatoxin B1 and nitrosamines. CYP2E1 metabolizes ethanol and other low-molecular weight compounds and can also activate nitrosamines. The CYP2A6 and CYP2E1 genes are polymorphic, altering their catalytic activities and susceptibility to cancer and other diseases. A number of polymorphisms described are ethnic-dependent. In the present study, we determined the genotype and allele frequencies of the main CYP2A6 and CYP2E1 polymorphisms in a group of 289 volunteers recruited at the Central Laboratory of Hospital Universitário Pedro Ernesto. They had been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP2A6; CYP2E1; Polymorphism; Brazil; Ethnic groups.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200005

Association between Toll-like receptor 4 Asp299Gly polymorphism and coronary heart disease susceptibility
Provedor de dados: 56
Autores: Wu,B.W.; Zhu,J.; Shi,H.M.; Jin,B.; Wen,Z.C.
Published data on the association between Toll-like receptor 4 (TLR4) Asp299Gly polymorphism and coronary heart disease (CHD) susceptibility are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. English-language studies were identified by searching PubMed and Embase databases (up to November 2016). All epidemiological studies were regarding Caucasians because no TLR4 Asp/Gly and Gly/Gly genotypes have been detected in Asians. A total of 20 case-control studies involving 14,416 cases and 10,764 controls were included in the meta-analysis. Overall, no significant associations were found between TLR4 Asp299Gly polymorphism and CHD susceptibility in the dominant model (OR=0.89; 95%CI=0.74 to 1.06; P=0.20)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toll-like receptor 4; Polymorphism; Coronary heart disease; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900609

Meta-analysis of the association between PADI4 -92C/G polymorphism and rheumatoid arthritis in the Chinese population
Provedor de dados: 56
Autores: Guo,Z.Y.; Zhang,J.X.; Wu,M.; Mei,Y.F.; Lin,X.J.; Bu,C.; Xie,Y.; Wang,J.
Many studies have evaluated the correlation between peptidylarginine deiminase 4 (PADI4) -92C/G polymorphism and rheumatoid arthritis (RA), but the results remain inconclusive. Therefore, we performed a meta-analysis in the Chinese population to provide comprehensive data on the association between PADI4 -92C/G polymorphism and RA. Eligible studies published before May 2016 were identified in PubMed and Chinese databases. The strengths of these associations were assessed by pooled odds ratios (OR) and 95% confidence interval (CI). Eight studies documenting a total of 1351 RA cases and 1585 controls were included in this meta-analysis. In the overall analysis, a significant association between the PADI4 -92C/G polymorphism and RA was found in the Chinese...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meta-analysis; Peptidylarginine deiminase 4; Polymorphism; Rheumatoid arthritis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000605

Mutations in the HFE gene and sporadic amyotrophic lateral sclerosis risk: a meta-analysis of observational studies
Provedor de dados: 56
Autores: Li,M.; Wang,L.; Wang,W.; Qi,X.L.; Tang,Z.Y.
Iron homeostasis dysregulation has been regarded as an important mechanism in neurodegenerative diseases. The H63D and C282Y polymorphisms in theHFE gene may be involved in the development of sporadic amyotrophic lateral sclerosis (ALS) through the disruption of iron homeostasis. However, studies investigating the relationship between ALS and these two polymorphisms have yielded contradictory outcomes. We performed a meta-analysis to assess the roles of the H63D and C282Y polymorphisms of HFEin ALS susceptibility. PubMed, MEDLINE, EMBASE, and Cochrane Library databases were systematically searched to identify relevant studies. Strict selection criteria and exclusion criteria were applied. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyotrophic lateral sclerosis; HFE; C282Y; H63D; Polymorphism; Meta-analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300215

Correlation between interleukin-18promoter -607C/A polymorphism and susceptibility to ischemic stroke
Provedor de dados: 56
Autores: Lu,Jian-xia; Lu,Zhong-qian; Zhang,Shao-lan; Zhi,Juan; Chen,Zheng-ping; Wang,Wan-xiang.
Single nucleotide polymorphisms in the promoter region ofinterleukin-18 (IL-18), an inflammatory cytokine, have been linked to susceptibility to many diseases, including cancer and immune dysfunction. Here, we explored the potential association between theIL-18 -607C/A (rs1946518) promoter region polymorphism and susceptibility to ischemic stroke (IS). This locus was amplified from peripheral blood samples of 386 IS patients (cases) and 364 healthy individuals (controls) by the polymerase chain reaction with sequence-specific primers. Significant differences were observed by the χ2 test in the -607C/A (rs1946518) genotype and allele frequencies between cases and controls (P &lt; 0.05). Furthermore, after excluding for age, gender, smoking status, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-18; Polymorphism; Ischemic stroke; Correlation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600502

Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
Provedor de dados: 56
Autores: Verbisck,N.V.; Da-Silva,S.; Mortara,R.A.
We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Amastigote; Monoclonal antibodies; Surface antigen; Polymorphism.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200011

Typing class I HLA-A gene using a nested PCR-RFLP procedure
Provedor de dados: 56
Autores: Castelli,E.C.; Gil,D.S.; Veiga,L.C.S.; de Camargo,J.L.V.
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human leukocyte antigen-A; HLA-A; Typing; RFLP; Polymorphism; Major histocompatibility complex.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004

HLA-DR3 antigen in the resistance to idiopathic dilated cardiomyopathy
Provedor de dados: 56
Autores: Jin,B.; Wu,B.W.; Wen,Z.C.; Shi,H.M.; Zhu,J.
Idiopathic dilated cardiomyopathy (IDC) has been hypothesized as a multifactorial disorder initiated by an environment trigger in individuals with predisposing human leukocyte antigen (HLA) alleles. Published data on the association between HLA-DR3 antigen and IDC risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Studies were identified by searching the PUBMED and Embase database (starting from June 2015). A total of 19 case-control studies including 1378 cases and 10383 controls provided data on the association between HLA-DR3 antigen and genetic susceptibility to IDC. Overall, significantly decreased frequency of HLA-DR3 allele (OR=0.72; 95%CI=0.58-0.90; P=0.004) was found in patients with IDC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-DR; Dilated cardiomyopathy; Polymorphism; Meta-analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400708

Insect juvenile hormone: from "status quo" to high society
Provedor de dados: 56
Autores: Hartfelder,K.
Juvenile hormone (JH) exerts pleiotropic functions during insect life cycles. The regulation of JH biosynthesis by neuropeptides and biogenic amines, as well as the transport of JH by specific binding proteins is now well understood. In contrast, comprehending its mode of action on target organs is still hampered by the difficulties in isolating specific receptors. In concert with ecdysteroids, JH orchestrates molting and metamorphosis, and its modulatory function in molting processes has gained it the attribute "status quo" hormone. Whereas the metamorphic role of JH appears to have been widely conserved, its role in reproduction has been subject to many modifications. In many species, JH stimulates vitellogenin synthesis and uptake. In mosquitoes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile hormone; Corpora allata; Metamorphosis; Insect reproduction; Polymorphism; Honey bee.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200003

Genetic variation of aggrecanase-2 (ADAMTS5) in susceptibility to osteoarthritis
Provedor de dados: 56
Autores: Zhou,Xindie; Jiang,Lifeng; Zhang,Yi; Zhang,Junjie; Zhou,Dong; Wu,Lidong; Huang,Yong; Xu,Nanwei.
Aggrecanase-2 (ADAMTS5) gene is responsible for aggrecan degradation that may contribute to cartilage destruction in a mouse osteoarthritis (OA) model. We aimed to investigate the effects of ADAMTS5 gene polymorphisms on OA risk in a Chinese population. A total of 300 OA patients and 300 controls were recruited and their genotypes for ADAMTS5 gene rs226794 and rs2830585 polymorphisms were determined using a custom-by-design 48-Plex single nucleotide polymorphism Scan™ kit. ADAMTS5-associated genes were identified by co-expression analysis and their functions were investigated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. Bioinformatics analysis showed that ADAMTS5 was significantly related to the components, structural constituent,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADAMTS5; Polymorphism; Osteoarthritis; Co-expression analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200602

Association of the 894G&gt;T polymorphism of the endothelial constitutive nitric oxide synthase gene with unstable angina
Provedor de dados: 56
Autores: Iturry-Yamamoto,G.R.; Moriguchi,E.H.; Zago,A.C.; Alho,C.S.; Zago,A.J.
The 894G&gt;T polymorphism of the endothelial constitutive nitric oxide synthase gene consists of the substitution of a guanine base by a thymine at the 894th nucleotide of the gene. An association of this polymorphism with acute coronary syndromes has been described, only when in combination with other polymorphisms of this gene. The aim of the present study was to search for an association between this polymorphism and unstable angina in a southern Brazilian population. In a case-control study, 156 patients (group 1 (N = 83): unstable angina, group 2 (N = 73): stable angina) were genotyped by PCR and digestion of the product. Univariate analysis demonstrated that the minimal luminal diameter and the degree of stenosis of the culprit lesion differed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Gene; Polymorphism; Unstable angina; Coronary.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400005

Association between tumor necrosis factor alpha rs1800629 polymorphism and risk of osteoarthritis in a Chinese population
Provedor de dados: 56
Autores: Chen,Jie; Wu,Yu; Yu,Jiannong; Shen,Jinming.
Osteoarthritis (OA) is the most common degenerative disease affecting articular cartilage. Some studies indicate that tumor necrosis factor alpha (TNF-α) gene rs1800629 polymorphism was associated with OA risk among Caucasian populations. To examine the role of this candidate gene in Asian populations, we conducted a hospital-based case-control study involving 257 knee OA patients and 305 controls in a Chinese population. Genotyping was performed using a custom-by-design 48-Plex single nucleotide polymorphism (SNP) Scan™ kit. Our study indicated that the AA genotype of TNF-α rs1800629 polymorphism was associated with increased risk of OA. Subsequently, we conducted a meta-analysis and found that rs1800629 polymorphism increased the risk of OA in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-α; Osteoarthritis; Polymorphism; Meta-analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800604

The MTR A2756G polymorphism is associated with an increase of plasma homocysteine concentration in Brazilian individuals with Down syndrome
Provedor de dados: 56
Autores: Biselli,J.M; Goloni-Bertollo,E.M; Haddad,R; Eberlin,M.N; Pavarino-Bertelli,E.C.
Individuals with Down syndrome (DS) present decreased homocysteine (Hcy) concentration, reflecting a functional folate deficiency secondary to overexpression of the cystathionine ß-synthase gene. Since plasma Hcy may be influenced by genetic polymorphisms, we evaluated the influence of C677T and A1298C polymorphisms in the methylenetetrahydrofolate reductase gene (MTHFR), of A2756G polymorphism in the methionine synthase gene (MTR), and of A80G polymorphism in the reduced folate carrier 1 gene on Hcy concentrations in Brazilian DS patients. Fifty-six individuals with free trisomy 21 were included in the study. Plasma Hcy concentrations were measured by liquid chromatography_tandem mass spectrometry with linear regression coefficient r² = 0.9996, average...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Down syndrome; Trisomy 21; Polymorphism; Homocysteine; Mass spectrometry (LC-MS/MS).
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100006

5-Methyltetrahydrofolate-homocysteine methyltransferase gene polymorphism (MTR) and risk of head and neck cancer
Provedor de dados: 56
Autores: Galbiatti,A.L.S.; Ruiz,M.T.; Biselli-Chicote,P.M.; Raposo,L.S.; Maniglia,J.V.; Pavarino-Bertelli,E.C.; Goloni-Bertollo,E.M.
The functional effect of the A&gt;G transition at position 2756 on the MTR gene (5-methyltetrahydrofolate-homocysteine methyltransferase), involved in folate metabolism, may be a risk factor for head and neck squamous cell carcinoma (HNSCC). The frequency of MTR A2756G (rs1805087) polymorphism was compared between HNSCC patients and individuals without history of neoplasias. The association of this polymorphism with clinical histopathological parameters was evaluated. A total of 705 individuals were included in the study. The polymerase chain reaction-restriction fragment length polymorphism technique was used to genotype the polymorphism. For statistical analysis, the chi-square test (univariate analysis) was used for comparisons between groups and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Head and neck cancer; Polymorphism; Folate metabolism; MTR gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500005

Bone mineral density in young women of the city of São Paulo, Brazil: correlation with both collagen type I alpha 1 gene polymorphism and clinical aspects
Provedor de dados: 56
Autores: Barros,E.R.; Kasamatsu,T.S.; Ramalho,A.C.; Hauache,O.M.; Vieira,J.G.H.; Lazaretti-Castro,M.
Osteoporosis is a multifactorial disease with great impact on morbidity and mortality mainly in postmenopausal women. Although it is recognized that factors related to life-style and habits may influence bone mass formation leading to greater or lower bone mass, more than 85% of the variation in bone mineral density (BMD) is genetically determined. The collagen type I alpha 1 (COLIA1) gene is a possible risk factor for osteoporosis. We studied a population of 220 young women from the city of São Paulo, Brazil, with respect to BMD and its correlation with both COLIA1 genotype and clinical aspects. The distribution of COLIA1 genotype SS, Ss and ss in the population studied was 73.6, 24.1 and 2.3%, respectively. No association between these genotypes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COLIA1; Bone mineral density; Risk factors; Polymorphism; Osteoporosis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800005

Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population
Provedor de dados: 56
Autores: Velavan,T.P.; Bechlars,S.; Huang,X.; Kremsner,P.G.; Kun,J.F.J.
Parasites are accountable for driving diversity within immune gene families. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the tumor necrosis factor receptor superfamily member 18 (TNFRSF18) gene by direct sequencing in a group of male Gabonese individuals exposed to a wide array of parasitic diseases such as malaria, filariasis and schistosomiasis. Two new promoter variants were identified in 40 individuals. Both novel variants were heterozygous and were linked to SNP #rs3753344 (C/T), which has been described. One of the SNP variants (ss2080581728) was close to the general transcription factor site, the TATA box. We further validated these new promoter variants for their allelic gene...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tumor necrosis factor receptor superfamily; Polymorphism; Transfection; Regulatory T cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500006

The P48T germline mutation and polymorphism in the CDKN2A gene of patients with melanoma
Provedor de dados: 56
Autores: Huber,J.; Ramos,E.S.
CDKN2A has been implicated as a melanoma susceptibility gene in some kindreds with a family history of this disease. Mutations in CDKN2A may produce an imbalance between functional p16ink4a and cyclin D causing abnormal cell growth. We searched for germline mutations in this gene in 22 patients with clinical criteria of hereditary cancer (early onset, presence of multiple primary melanoma or 1 or more first- or second-degree relatives affected) by secondary structural content prediction, a mutation scanning method that relies on the propensity for single-strand DNA to take on a three-dimensional structure that is highly sequence dependent, and sequencing the samples with alterations in the electrophoretic mobility. The prevalence of CDKN2A mutation in our...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CDKN2A; Familial melanoma; Mutation; P48T; Polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200010

Association between MCP-1 -2518A&amp;gt;G polymorphism and asthma susceptibility: a meta-analysis
Provedor de dados: 56
Autores: Chen,Wenli; Cui,Jiewei; Xiang,Guoan; Zhang,Jianpeng; Gao,Hongmei.
The published data on the association between MCP-1 -2518A&gt;G polymorphism and asthma susceptibility are inconclusive. Therefore, we performed a meta-analysis to estimate the impact of MCP-1 -2518A&gt;G polymorphism on asthma susceptibility. PubMed, Web of Science, Wanfang, and China National Knowledge Infrastructure (CNKI) databases were used to identify eligible studies. The pooled odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were used to calculate the strength of association. Sensitivity analysis was performed to evaluate the influence of individual studies on the estimates of overall effect, and funnel plots and Egger's test were used to assess publication bias. Eight publications with 1562 asthma patients and 1574 controls were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MCP-1; Polymorphism; Asthma; Susceptibility; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100602

Genetic variability of vascular endothelial growth factor and prognosis of head and neck cancer in a Brazilian population
Provedor de dados: 56
Autores: Ruiz,M.T.; Biselli,P.M.; Maniglia,J.V.; Pavarino-Bertelli,E.C.; Goloni-Bertollo,E.M.
Vascular endothelial growth factor (VEGF) is one of the most potent endothelial cell mitogens and plays a critical role in angiogenesis. Polymorphisms in this gene have been evaluated in patients with several types of cancer. The objectives of this study were to determine if there was an association of the -1154G/A polymorphism of the VEGF gene with head and neck cancer and the interaction of this polymorphism with lifestyle and demographic factors. Additionally, the distribution of the VEGF genotype was investigated with respect to the clinicopathological features of head and neck cancer patients. The study included 100 patients with histopathological diagnosis of head and neck squamous cell carcinoma. Patients with treated tumors were excluded. A total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Head and neck cancer; Polymorphism; Genetic; VEGF gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200002

Association of apolipoprotein E polymorphism with plasma lipids and Alzheimer's disease in a Southern Brazilian population
Provedor de dados: 56
Autores: de-Andrade,F.M.; Larrandaburu,M.; Callegari-Jacques,S.M.; Gastaldo,G.; Hutz,M.H.
Apolipoprotein E (protein: apo E; gene: APOE) plays an important role in the multifactorial etiology of both Alzheimer's disease (AD) and lipid level concentrations. The polymerase chain reaction (PCR) was used to investigate the APOE gene polymorphism in 446 unrelated Caucasians, among them 23 AD patients, and 100 Afro-Brazilians living in Porto Alegre, Brazil. The frequencies of the APOE*2, APOE*3 and APOE*4 alleles were 0.075, 0.810 and 0.115 in Caucasians and 0.075, 0.700 and 0.225 in Afro-Brazilians, respectively (c2 = 8.72, P = 0.013). A highly significant association was observed between the APOE*4 allele and AD in this population-based sample. The APOE*4 frequency in AD patients (39%) was about four times higher than in the general Caucasian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; APOE; Lipoproteins; Apoprotein; Alzheimer's disease.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500007

Genetic susceptibility to HPV infection and cervical cancer
Provedor de dados: 56
Autores: Maciag,P.C.; Villa,L.L.
Squamous cell carcinoma of the cervix (SCCC) is one of the leading causes of death in developing countries. Infection with high-risk human papillomavirus (HPV) is the major risk factor to develop malignant lesions in the cervix. Polymorphisms of the MHC and p53 genes seem to influence the outcome of HPV infection and progression to SCCC, although controversial data have been reported. MHC are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in immune regulation, while p53 is a tumor suppressor gene that regulates cell proliferation. The HPV E6 protein from high-risk types binds p53 and mediates its degradation by the ubiquitin pathway. The role of these polymorphisms in genetic susceptibility to HPV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical cancer; HLA; P53; Polymorphism.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700017

Polymorphism of the aryl-hydrocarbon receptor gene in intron 10 of human cancers
Provedor de dados: 56
Autores: Rocas,M.; Jakubauskiene,E.; Kanopka,A.
Polychlorinated dibenzo-p-dioxins (PCDDs) and related halogenated aromatic hydrocarbons (e.g., PCDFs), often called "dioxins", are ubiquitously present environmental contaminants. Some of them, notably 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), are among the most toxic synthetic compounds known. The biological effects of dioxins are mediated via the aryl hydrocarbon receptor (AhR). Mutations in the AhR transactivation domain are linked to sensitivity to the acute lethality of TCDD. We present here a study of AhR gene polymorphism in normal and cancer human tissues affecting pre-mRNA splicing in the AhR gene-coding transactivation domain region (exon 10, intron 10, exon 11 region), previously shown to be associated with AhR dysfunction. We tested 126 pairs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ah receptor; Polymorphism; Splicing; Cancer; Dioxins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100006

ABCB1 C1236T, G2677T/A and C3435T polymorphisms in systemic lupus erythematosus patients
Provedor de dados: 56
Autores: Gonzalez,T.P.; Mucenic,T.; Brenol,J.C.T.; Xavier,R.M.; Schiengold,M.; Chies,J.A.B.
P-glycoprotein (Pgp), the ABCB1 gene product, acts as an efflux pump that transports a large variety of substrates and is a mechanism of cell protection against xenobiotics. An increasing number of studies have shown that some ABCB1 polymorphisms may affect Pgp expression and activity, as well as affecting the development and susceptibility to diseases and pharmacological response. High activity of Pgp has been detected in systemic lupus erythematosus (SLE) patients. The C1236T, G2677T/A, and C3435T are the most commonly studied single nucleotide polymorphisms in the ABCB1 gene. Therefore, their frequencies were determined in Brazilian individuals with European ancestry (N = 143) and in SLE patients (N = 137). Genotyping was performed by PCR-RFLP analysis...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: ABCB1; MDR1; P-glycoprotein; Polymorphism; Systemic lupus erythematosus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900005

Genetic correlation between Prothrombin G20210A polymorphism and retinal vein occlusion risk
Provedor de dados: 56
Autores: Zou,Yuanyuan; Zhang,Xi; Zhang,Jingyi; Ji,Xiangning; Liu,Yuqing; Zhao,Shaozhen.
The aim of this study was to perform an updated meta-analysis to quantitatively investigate the association between G20210A polymorphism of Prothrombin gene and the risk of retinal vein occlusion (RVO), based on the available publications with inconsistent results. We utilized the Stata software to perform the heterogeneity test, association test, Begg's and Egger's tests, and sensitivity analysis. We searched three on-line databases (PubMed, Embase, and WOS) and obtained a total of 422 articles. Based on our selection criteria, 24 case-control studies were finally enrolled in this overall meta-analysis; a subgroup analysis by the factors ethnicity, control source, and RVO type was done. Through the association test of overall meta-analysis, we did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prothrombin; Retinal vein occlusion; Polymorphism; Risk; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400604

A novel polymorphism in the coding region of the vasopressin type 2 receptor gene
Provedor de dados: 56
Nephrogenic diabetes insipidus (NDI) is a rare disease characterized by renal inability to respond properly to arginine vasopressin due to mutations in the vasopressin type 2 receptor (V2(R)) gene in affected kindreds. In most kindreds thus far reported, the mode of inheritance follows an X chromosome-linked recessive pattern although autosomal-dominant and autosomal-recessive modes of inheritance have also been described. Studies demonstrating mutations in the V2(R) gene in affected kindreds that modify the receptor structure, resulting in a dys- or nonfunctional receptor have been described, but phenotypically indistinguishable NDI patients with a structurally normal V2(R) gene have also been reported. In the present study, we analyzed exon 3 of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Vasopressin; Receptor; Polymorphism; Diabetes insipidus; Receptor gene polymorphism.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400002

Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
Provedor de dados: 58
Autores: Gibotti,Adriana; Tanaka,Tânia L.; Oliveira,Valéria R.; Taddei,Carla R.; Martinez,Marina B.
In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipa; Enteroinvasive Escherichia coli; Polymorphism; PCR-RFLP.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012

DEFB1 gene polymorphisms and tuberculosis in a Northeastern Brazilian population
Provedor de dados: 58
Autores: Silva,Ronaldo Celerino da; Cruz,Heidi Lacerda Alves da; Brandão,Lucas André Cavalcanti; Guimarães,Rafael Lima; Montenegro,Lilian Maria Lapa; Schindler,Haiana Charifker; Segat,Ludovica; Crovella,Sergio.
Abstract β-Defensin-1, an antimicrobial peptide encoded by the DEFB1 gene, is known to play an important role in lung mucosal immunity. In our association study we analyzed three DEFB1 functional polymorphisms -52G&gt;A (rs1799946), -44C&gt;G (rs1800972) and -20G&gt;A (rs11362) in 92 tuberculosis patients and 286 healthy controls, both from Northeast Brazil: no association was found between the studied DEFB1 polymorphisms and the disease. However we cannot exclude that this lack of association could be due to the low number of subjects analyzed, as suggested by the low statistical power achieved for the three analyzed SNPs (values between 0.16 and 0.50).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Innate immunity; DEFB1; Genotyping; Polymorphism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200389

Sleep quality and OPRM1 polymorphisms: a cross-sectional study among opioid-naive individuals
Provedor de dados: 60
Autores: Zahari,Zalina; Ibrahim,Muslih Abdulkarim; Musa,Nurfadhlina; Tan,Soo Choon; Mohamad,Nasir; Ismail,Rusli.
ABSTRACT Opioidergic system involves in regulation of sleep and wakefulness. It is possible, therefore, that genetic polymorphisms in OPRM1 influence sleep quality. This study investigated the association of OPRM1 polymorphisms with subjective sleep quality among opioid-naive individuals. This cross-sectional observational study involved 161 opioid-naive males (mean age = 27.74 years; range: 18−63 years). Subjective sleep quality was assessed with the translated and validated Malay version of the Pittsburgh Sleep Quality Index (PSQI). DNA was extracted from whole blood and subjected to polymerase chain reaction (PCR)-genotyping for two OPRM1 polymorphisms (118A&gt;G and IVS2+691G&gt;C). Subjects with combined 118A and IVS2+691G alleles (AC haplotype) had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep-Wake Transition Disorders; Sleep quality; Sleep/genetic; Polymorphism; Pittsburgh Sleep Quality Index; OPRM1; Opioid receptors /m analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100610

Polymorphism: an evaluation of the potential risk to the quality of drug products from the Farmácia Popular Rede Própria
Provedor de dados: 60
Autores: Santos,Olímpia Maria Martins; Reis,Maria Esther Dias; Jacon,Jennifer Tavares; Lino,Mônica Esselin de Sousa; Simões,Juliana Savioli; Doriguetto,Antonio Carlos.
Polymorphism in solids is a common phenomenon in drugs, which can lead to compromised quality due to changes in their physicochemical properties, particularly solubility, and, therefore, reduce bioavailability. Herein, a bibliographic survey was performed based on key issues and studies related to polymorphism in active pharmaceutical ingredient (APIs) present in medications from the Farmácia Popular Rede Própria. Polymorphism must be controlled to prevent possible ineffective therapy and/or improper dosage. Few mandatory tests for the identification and control of polymorphism in medications are currently available, which can result in serious public health concerns.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Medicines/quality control; Medicines/solubility; Medicines/bioavailability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100002

Thermoanalytical studies of carbamazepine: hydration/dehydration, thermal decomposition, and solid phase transitions
Provedor de dados: 60
Autores: Pinto,Mônia Aparecida Lemos; Ambrozini,Beatriz; Ferreira,Ana Paula Garcia; Cavalheiro,Éder Tadeu Gomes.
Carbamazepine (CBZ), a widely used anticonvulsant drug, can crystallize and exhibits four polymorphic forms and one dihydrate. Anhydrous CBZ can spontaneously absorb water and convert to the hydrate form whose different crystallinity leads to lower biological activity. The present study was concerned to the possibility of recovering the hydrated form by heating. The thermal behavior of spontaneously hydrated carbamazepine was investigated by TG/DTG-DTA and DSC in dynamic atmospheres of air and nitrogen, which revealed that the spontaneous hydration of this pharmaceutical resulted in a Form III hydrate with 1.5 water molecules. After dehydration, this anhydrous Form III converted to Form I, which melted and decomposed in a single event, releasing isocyanic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbamazepine/thermal analysis; Polymorphism; Hydrate; Dehydration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400877

Assessment of analytical techniques for characterization of crystalline clopidogrel forms in patent applications
Provedor de dados: 60
Autores: Lira,Luiz Marcelo; Lourenço,Alexandre Lopes; Neves,Guilherme Ferreira; Sousa,Valéria Pereira de; Rodrigues,Carlos Rangel; Cabral,Lúcio Mendes.
The aim of this study was to evaluate two important aspects of patent applications of crystalline forms of drugs: (i) the physicochemical characterization of the crystalline forms; and (ii) the procedure for preparing crystals of the blockbuster drug clopidogrel. To this end, searches were conducted using online patent databases. The results showed that: (i) the majority of patent applications for clopidogrel crystalline forms failed to comply with proposed Brazilian Patent Office guidelines. This was primarily due to insufficient number of analytical techniques evaluating the crystalline phase. In addition, some patent applications lacked assessment of chemical/crystallography purity; (ii) use of more than two analytical techniques is important; and (iii)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clopidogrel/crystalline forms/physicochemical characterization; Clopidogrel/crystalline forms/patents; Polymorphism.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200229

Effect of MDR C3435T polymorphism on Varenicline treatment in quit smoking
Provedor de dados: 60
Autores: Bozkurt,Nurgul; Altıntas,Fatih; Bozkurt,Ali Ihsan; Turgut,Gunfer; Turgut,Sebahat.
Despite so many global efforts, smoking still remains to be one of the most common addictions worldwide. Even though most smokers wish to quit smoking, many of them fail. In this respect, genetic variants are thought to be remarkable factors in nicotine dependence and in treatment of smoking cessation. This is a paper investigating a single variant p-glycoprotein (P-gp) polymorphisms and its effect on Varenicline efficacy in the smoking cessation. 158 smokers and 52 non-smoker healthy volunteers were included. We determined the P-gp C3435T gene polymorphisms in all subjects. Face to face interviews with smokers were performed for smoking cessation and Varenicline was given for smoking cessation. Cessation success was evaluated in the 6th month and success...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Smoking Cessation/methods; Varenicline/analysis; Varenicline/adverse effects; Smoking/genetics; Genetic/drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100506

Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines
Provedor de dados: 65
Autores: Possamai,Mari Helen Pagani; Battilana,Jaqueline; Paludo,Ediane; Herkenhoff,Marcos Edgar; Pértile,Fábio; Lima-Rosa,Carlos André da Veiga.
This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraíso Pedrês (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: (Caipira) broiler line; (Caipira) layer line; Microsatellite; Polymorphism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877

Sexual castes of Trachymyrmex fuscus (Formicidae: Attini) performing worker tasks
Provedor de dados: 65
Autores: Araújo,Márcio da Silva; Oliveira,João Paulo Ribeiro; Souza,Danival José de; Oliveira,Marco Antônio; Jesus,Flávio Gonçalves de.
ABSTRACT: The main of this research was to report an atypical foraging behavior in a colony of Trachymyrmex fuscus, situated in "Cerrado" (savanna ecoregion of Goiás, Brazil). The colony foraging activity was performed only by sexual caste. Comparison of the foraging rhythm of this colony with another of the same specie where foraging was performed only by worker caste, showed that working time hours were very similar. After observations on the foraging behavior, both colonies were excavated in order to characterize them (nest size, population composition and estimating of the symbiotic fungus volume). Besides the foraging activity performed only by sexual females, other important observations were highlighted in that colony: low number of workers,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungus-growing ant; Foraging activity; Division of labour; Polymorphism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200199