Consulta: subjectFacets:"Response Surface Methodology"
Registros recuperados: 9
Data/hora: 05/06/2024 12:47:46

Optimization of the pulsed electric field -assisted extraction of functional compounds from Nepeta binaludensis
Provedor de dados: 111
Autores: Azimi Mahalleh, Afsaneh; Sharayei, Parvin; Mortazavi, Seyed Ali; Azarpazhooh, Elham; Niazmand, Razieh.
Pulsed electric field (PEF) treatment was used for extracting effective components from Nepeta (Nepeta binaludensis Jamzad). A response surface method was used to investigate the effects of independent process variables (voltage of pulsed electric field (VPEF): 2, 4 and 6 KV/cm and number of pulsed electric field (NPEF): 20, 40 and 60 n) on the yield (Y) and antioxidant characteristics: total phenolic compounds (TPC), 1,1- diphenyl -2- picrylhydrazyl free radical scavenging (DPPHsc), ferric reducing-antioxidant power (FRAP), half  maximal of radical-scavenging activity (IC50) of Nepeta extract (aerial parts). According to Derringer’s desired function approach, the optimal conditions based on both individual and combinations of all process variables were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic compounds; Nepeta binaludensis; Response Surface Methodology; Pulsed electric field; Antimicrobial activity.
Ano: 2019
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5391

Optimization of the pulsed electric field -assisted extraction of functional compounds from Nepeta binaludensis
Provedor de dados: 111
Autores: Azimi Mahalleh, Afsaneh; Sharayei, Parvin; Mortazavi, Seyed Ali; Azarpazhooh, Elham; Niazmand, Razieh.
Pulsed electric field (PEF) treatment was used for extracting effective components from Nepeta (Nepeta binaludensis Jamzad). A response surface method was used to investigate the effects of independent process variables (voltage of pulsed electric field (VPEF): 2, 4 and 6 KV/cm and number of pulsed electric field (NPEF): 20, 40 and 60 n) on the yield (Y) and antioxidant characteristics: total phenolic compounds (TPC), 1,1- diphenyl -2- picrylhydrazyl free radical scavenging (DPPHsc), ferric reducing-antioxidant power (FRAP), half  maximal of radical-scavenging activity (IC50) of Nepeta extract (aerial parts). According to Derringer’s desired function approach, the optimal conditions based on both individual and combinations of all process variables were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic compounds; Nepeta binaludensis; Response Surface Methodology; Pulsed electric field; Antimicrobial activity.
Ano: 2019
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5391

Extraction of pectin from apple pomace
Provedor de dados: 52
Autores: Canteri-Schemin,Maria Helene; Fertonani,Heloísa Cristina Ramos; Waszczynskyj,Nina; Wosiacki,Gilvan.
As apple-processing units are now in expansion in Brazil, industrial by-products like pomace play an important role in pectin manufacture. The objective of this article was to determine a pratical follow-up to the extraction of pectin from apple pomace and to characterize it in a laboratory, on a small scale, aiming at establishing the optimum conditions for acid extraction. The highest yields were obtained when [1]apple pomace was dried and ground to obtain an apple flour to be used as raw material, [2] citric or nitric acids were used and [3] when the citric acid concentration was 6.2 g/100 ml and the time of reaction was 153 minutes. The apple variety in itself was not significant in pectin yield. The degree of esterification (DE = 68.84 %) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple pomace; Pectin; Agricultural wastes; Yield; Extraction; Response Surface Methodology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200013

Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation
Provedor de dados: 52
Autores: Mustafa,Siti Ratina; Husaini,Ahmad; Hipolito,Cirilo Nolasco; Hussain,Hasnain; Suhaili,Nurshikin; Roslan,Hairul Azman.
Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH9 employing response surface methodology (RSM).Central composite design (CCD) was applied to determine the optimal fermentation condition with respect to the four main process parameters such as temperature, initial moisture content, pH and the incubation period. Solid state fermentation (SSF) was performed using 5.0 g of sago hampas inoculated with 1x107sporesmL-1following the experimental design obtained using CCD and further optimized by RSM. The initial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid state fermentation; Aspergillus flavusNSH9; Sago hampas; Central Composite Design; Response Surface Methodology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100416

Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
Provedor de dados: 52
Autores: Kumari,Annapurna; Mahapatra,Paramita; Banerjee,Rintu.
Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central composite design; Enterobacter aerogenes; Lipase; Optimization; Parameter; Response Surface Methodology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005

A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol
Provedor de dados: 52
Autores: Anschau,Andréia; Santos,Lucielen Oliveira dos; Alegre,Ranulfo Monte.
This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione; Fermentation; Fed Batch Culture; Amino Acids; Response Surface Methodology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500017

Statistical Optimisation of Fermentation Media for Novel Clostridium novyi -NT Using Response Surface Methodology.
Provedor de dados: 52
Autores: Krishnamoorthy,Kannan; Landge,Anil Vijay.
ABSTRACT Clostridial collagenase is recognized as one of the important proteolytic enzymes used in treatment of varieties of fibro-proliferative disorders. The present work aimed to optimise the biomass concentration and collagenase enzyme activity of novel bacilli Clostridium novyi-NT. The response surface methodology tool was used to identify the optimal fermentation parameters. Central composite design (CCD) was applied with respect to three influencing factors - pH, proteose peptone and trypticase soya broth. These factors showed significant effect on the overall biomass concentration and collagenase enzyme activity (p&lt;0.05). The maximum biomass concentration in terms of absorbance and collagenase activity (of crude enzyme) was achieved and recorded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagenase; Clostridium novyi-NT; Fibro-proliferative disorders; Response Surface Methodology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100429

Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor
Provedor de dados: 52
Autores: Patel,Seema; Kothari,Damini; Shukla,Rishikesh; Das,Debasish; Goyal,Arun.
The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman Design and a 2² full factorial Central Composite Design was employed. After response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. The experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediococcus pentosaceus; Dextran; Plackett-Burman Design; Central Composite Design; Response Surface Methodology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600007

Comparative analyses of response surface methodology and artificial neural networks on incorporating tetracaine into liposomes
Provedor de dados: 60
Autores: Pereira,Ana Karina Vidal; Barbosa,Raquel de Melo; Fernandes,Marcelo Augusto Costa; Finkler,Leandro; Finkler,Christine Lamenha Luna.
This study evaluated the incorporation of tetracaine into liposomes by RSM (Response Surface Methodology) and ANN (Artificial Neural Networks) based models. RCCD (rotational central composite design) and ANN were performed to optimize the sonication conditions of particles containing 100 % lipid. Laser light scattering was used to perform measure hydrodynamic radius and size distribution of vesicles. The liposomal formulations were analyzed by incorporating the drug into the hydrophilic phase or the lipophilic phase. RCCD and ANN were conducted, having the lipid/cholesterol ratio and concentration of tetracaine as variables investigated and, the encapsulation efficiency and mean diameter of the vesicles as response variables. The optimum sonication...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Local anesthetics; Liposomes; Response Surface Methodology; Artificial Neural Networks.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100517