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Assessment of DNA extraction methods for detection of arbuscular mycorrhizal fungi in plant roots by nested-PCR
Provedor de dados: 3
Autores: Diédhiou, Abdala Gamby; Borges, Wardsson Lustrino; Sadio, Oumar; de Faria, Sergio Miana.
DNA extraction methods were evaluated for the yield and purity of DNA recovered from mycorrhized roots and whether the recovered DNA is suitable for amplification of arbuscular mycorrhizal (AM) fungal SSU rDNA. The DNeasy Plant Mini Kit and three extraction buffers were used alone or in combination with either polyvinylpyrrolidone (PVP), polyvinylpolypyrrolidone (PVPP) and/or activated charcoal (AC). Among the extraction methods tested, those based on the CTAB buffers yielded more DNA than those based on the TE buffer and the DNeasy Plant Mini Kit. Moreover, the use of AC alone or in combination with PVPP reduced DNA yield, while it significantly improved the purity of recovered DNA, whatever the extraction buffer. On the other hand, the success of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activated charcoal; TE buffer; DNA quality.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/21689