Consulta: subjectFacets:"Tissue culture"
Registros recuperados: 97
Data/hora: 01/06/2024 02:04:36

Transformación genética de cebolla (Allium cepa L.) mediante biobalística
Provedor de dados: 32
Autores: Quintana Sierra, María Elena.
El objetivo de la presente investigación fue desarrollar un protocolo de transformación  genética de cebolla mediante biobalística, para lo cual fue necesario establecer un  sistema eficiente de regeneración de cebolla in vitro.  La metodología desarrollada  permite la regeneración de plantas de las variedades Cristal y El Toro. La variedad Cristal  mostró mayor porcentaje de explantes con plantas y mayor número de plantas por  explante. Se obtuvieron plantas después de 16 semanas de haber iniciado el cultivo y  éstas formaron microbulbos, los cuales alcanzaron hasta 3 cm de diámetro cuando se  transplantaron en invernadero. La respuesta in vitro obtenida superó a la reportada por  otros autores. Mediante el sistema de bombardeo de alta presión, las...
Tipo: Tesis
Palavras-chave: Allium cepa; Callos embriogénicos; Cultivo de tejidos; Cotransformación; Doctorado; Genética; Onion; Embryogenic callus; Tissue culture; Cotransformation.
Ano: 2009
URL: http://hdl.handle.net/10521/1539

Cultivo in vitro de portainjertos tolerantes al virus de la tristesa en cítricos: Utilización en sustratos inertes
Provedor de dados: 32
Autores: Martínez Hernández, María de Jesús.
La multiplicación de plantas in vitro presenta varias etapas críticas, desde la selección  de los componentes para la germinación, multiplicación y enraizamiento hasta el  proceso de aclimatación en invernadero. En la presente investigación se utilizaron los  sustratos inertes (vermiculita, agrolita y tezontle) como medio de soporte en  sustitución del agar y los azúcares xilosa, fructosa y 10X20F se compararon con la  sacarosa, en su efecto sobre la germinación, multiplicación, enraizamiento y  sobrevivencia de los portainjertos de cítricos: Citrus volkameriana, Citrumelo swingle  y Citrange 35. Además de probar diferentes intensidades luz en el proceso de  aclimatación de plántulas de Citrus volkameriana con diferentes sustratos y una  reducción gradual...
Tipo: Tesis
Palavras-chave: Cultivo in vitro; Sustratos inertes; Cítricos; Doctorado; Agroecosistemas tropicales; Tissue culture; Inert substrates; Citrus.
Ano: 2007
URL: http://hdl.handle.net/10521/1263

Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
Provedor de dados: 69
Autores: Azadi,Pejman; Khosh-Khui,Morteza.
A protocol for the micropropagation in different harvesting time of Lilium ledebourii (Baker) Boiss, an endangered rare species endemic to Iran has been developed. In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. Among the various treatments tested, the Murashige and Skoog (MS) medium supplemented with 0.1 mg l-1 naphthaleneacetic acid (NAA) + 0.1 mg l-1 benzyladenin (BA) and 6% sucrose in all harvesting seasons proved to be superior to others. The best results for fresh weight of bulblets, rooting parameters and the survival rate after transplantation to greenhouse were obtained from early winter-harvested bulbs. Summer-harvested bulbs had the highest number of bulblets per...
Tipo: Journal article
Palavras-chave: Bulblet; Cold treatment; Conservation; Harvesting season; Lilium ledebourii; Tissue culture.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400011

Normalized embryoid cDNA library of oil palm (Elaeis guineensis)
Provedor de dados: 69
Autores: Chan,Pek-Lan; Ma,Lay-Sun; Low,Eng-Ti L; Shariff,Elyana M; Ooi,Leslie Cheng-Li; Cheah,Suan-Choo; Singh,Rajinder.
A normalized embryoid cDNA library (EON) was constructed based on reassociation kinetics reaction. Results from dot blot hybridization and sequencing of EON cDNA clones clearly indicated that the normalization process reduced the frequency of high abundance transcripts and increased the frequency of low abundance gene transcripts. A total of 553 non-redundant expressed sequence tags (ESTs) were identified, 325 of these were not observed in the standard oil palm cDNA libraries sequenced previously. A total of 10 EON cDNA clones were chosen for expression profiling across samples from different stages of the tissue culture process. Two of the genes exhibited promising expression patterns for predicting the embryogenic potential in callus. Some of these genes...
Tipo: Journal article
Palavras-chave: Auxin; Expressed sequence tags; Gene expression; Low abundance genes; Tissue culture.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100010

Salinity effects on protein content, lipid peroxidation, pigments, and proline in Paulownia imperialis (Siebold &amp;amp; Zuccarini) and Paulownia fortunei (Seemann &amp;amp; Hemsley) grown in vitro
Provedor de dados: 69
Autores: Ayala-Astorga,Gloria Irma; Alcaraz-Meléndez,Lilia.
We evaluated the effects of saline stress on soluble proteins, lipid peroxidation (TBAR), chlorophyll a, chlorophyll b, &amp;#946;-carotene, violaxanthin, and proline in Paulownia imperialis and Paulownia fortunei plants grown in vitro. When the propagated plants reached a determined size, they were transferred aseptically to WPM culture medium containing different sodium chloride concentrations (0, 20, 40, 60, 80, and 160 mM) and were sampled at 15 and 30 days. Proline content was determined at 30 days after transfer only. Protein concentration significantly decreased with the highest salt levels in P. imperialis compared to controls in which no sodium chloride was added. In both P. imperialis and P. fortunei, lipid peroxidation significantly increased at...
Tipo: Journal article
Palavras-chave: Paulownia fortunei; Paulownia imperialis; Pigments; Proline; Proteins; Salinity; TBARS; Tissue culture.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500013

Genetic and epigenetic effects of salinity on in vitro growth of barley
Provedor de dados: 74
Autores: Demirkiran,Aykut; Marakli,Sevgi; Temel,Aslihan; Gozukirmizi,Nermin.
Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hordeum vulgare; Salt stress; Tissue culture; RAPD; CRED-RA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400016

Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha
Provedor de dados: 75
Autores: Santa-Rosa,Sandra; Souza,Fernanda VD; Vidal,Ádila M; Ledo,Carlos Alberto da S; Santana,José RF de.
Aechmea blanchetiana and Aechmea distichantha are Brazilian native bromeliads with great ornamental value, although none of the species are produced commercially. Therefore, market demands are met through nature predatory exploitation. This study intended to (1) evaluate the in vitro seed germination of A. blanchetiana and A. distichantha and (2) establish micropropagation protocols for both species, aiming at producing plantlets for the market, as well as to germplasm in vitro conservation. Germination experiments were carried out in completely randomized designs with two treatments (presence and absence of light) and seven and eight replications respectively for A. blanchetiana and A. distichantha. In vitro seedlings, produced under light conditions,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliaceae; Tissue culture; In vitro multiplication rate; Growth regulators.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100018

Response of root explants to in vitro cultivation of marketable garlic cultivars
Provedor de dados: 75
Autores: Scotton,Danielle C; Benedito,Vagner Augusto; Molfetta,Jeanne B de; Rodrigues,Benedita Inês FP; Tulmann-Neto,Augusto; Figueira,Antonio.
Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitro regeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium sativum; Bulb; Callus induction; Organogenesis; Regeneration; Tissue culture.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100013

In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations
Provedor de dados: 75
Autores: Faria,Ricardo T. de; Rodrigues,Fabiana N.; Oliveira,Luciana do V.R.; Müller,Cláudio.
Sucrose is a very important component in in vitro culture media, serving as a source of carbon and energy. In this paper, the rooting and in vitro growth of Dendrobium nobile Lindl (Orchidaceae) were studied using different sucrose concentrations (0 g L-1; 5 g L-1; 10 g L-1; 20 g L-1; 30 g L-1 and 60 g L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.8. Greater increases in plant height (4.21±0.6 cm) and high seedling multiplication (1:4) were observed in the 60 g L-1 sucrose treatment, even without the addition of plant hormones. Sucrose concentration in the culture medium did not influence in vitro plant rooting.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrobium nobile; Orchidaceae; Carbohydrate; Tissue culture.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362004000400023

In vitro growth of Brassocattleya orchid hybrid in different concentrations of KNO3, NH4NO3 and benzylaminopurine
Provedor de dados: 75
Autores: Cardoso,Jean C; Ono,Elizabeth O.
One of the most important applications of plant tissue culture is mass propagation of ornamental plants. This experiment evaluated the effect of different concentrations of NH4NO3 and KNO3 and BAP on the in vitro growth of orchid hybrid Brassocattleya 'Pastoral'. Seedlings of this orchid hybrid were used as explants and cultivated in medium with mineral salts and vitamins from the MS medium (Murashige &amp;amp; Skoog, 1962), with the macronutrients P, Ca and Mg reduced by half, and with an addition of 25 g L-1 of sucrose, 0.1 g L-1 of myo-inositol and 1.5 g L-1 of activated charcoal. Agar-agar was added (6.5 g L-1) and the pH was adjusted to 5.8. As treatments, four concentrations of the NH4NO3 and KNO3 (2x; 1x; ½ and ¼ MS medium) and three concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Macronutrients; Cytokinins; Multiplication; Growth.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000300017

Estabelecimento in vitro de faveleira (Cnidoscolus quercifolius Pohl) sem espinhos.
Provedor de dados: 14
Autores: RIBEIRO, J. M.; MELO, N. F. de; OLIVEIRA, V. R. de.
O objetivo deste trabalho foi estabelecer e multiplicar, sob condições de cultivo in vitro, um acesso de faveleira sem espinhos, visando alternativas para sua conservação e uso futuro. Para isso, segmentos nodais e fragmentos de folhas jovens foram inseridos em meio nutritivo composto pela formulação de sais inorgânicos WPM. Para os segmentos nodais, o meio de cultura foi suplementado com as seguintes concentrações (mg/L) de BAP (6-benzilaminopurina): T1:0; T2:0,1; T3:0,5; T4:1,0 e T5:1,5. Já aquele em que foram utilizados fragmentos de folhas jovens como explantes, foram testadas as seguintes concentrações (mg/L) de 2,4-D (ácido 2,4-diclorofeno-xiacético): T1:0; T2:10; T3:20, T4:40 e T5:80. Decorridos 30 e 60 dias, foram tomados dados relacionados ao...
Tipo: Folhetos
Palavras-chave: Favela; Faveleira sem espinhos; Planta nativa; Espécie forrageira; Manutenção de germoplasma; Contaminação in vitro; Oxidação in vitro; Cultivo in vitro; Selo ODS 2; Bioma Caatinga; Caatinga; Propagação Vegetativa; Cultura de Tecido; Biodiversidade; Tecido Vegetal; Espécie Nativa; Cnidoscolus quercifolius; Vegetative propagation; Tissue culture.
Ano: 2022
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1147865

Propagando inovações para o florescimento de novos mercados: anais.
Provedor de dados: 14
Autores: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 22.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 9., 2019, Juazeiro, BA.
O 22º CBFPO &amp; 9º CBCTP se apresenta como fórum de discussão que envolve instituições de pesquisa, configurando uma importante ação de capacitação e atualização para profissionais e acadêmicos. Outro ator importante neste cenário é o setor empresarial, pela oportunidade de expor seus produtos e propor soluções e parcerias, visando a ampliação do setor produtivo.
Tipo: Livro técnico (INFOTECA-E)
Palavras-chave: Novo mercado; Cultura de Tecido; Planta Ornamental; Floricultura; Flor Tropical; Tissue culture; Ornamental plants; Floriculture; Floriculture crops.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1119348

Influência do pH, concentração de carvão ativado e volume de meio nutritivo sobre a multiplicação in vitro de palma-forrageira [Opuntia tuna (L.) Mill.].
Provedor de dados: 14
Autores: RIBEIRO, J. M.; TEIXEIRA, S. L.
Este trabalho foi desenvolvido com o objetivo de otimizar o protocolo de clonagem in vitro de palma por meio de testes com pH, uso de carvão ativado e volume de meio de cultura que ainda não haviam sido testados para essa espécie. Para verificar o efeito do pH do meio de cultura sobre o desenvolvimento in vitro da palma, foram testados os pHs de 3,7; 4,7; 5,7; 6,7 e 7,7. O efeito do carvão ativado sobre o desenvolvimento in vitro de palma foi avaliado testando-se as concentrações 0 mg L-1; 40 mg L-1; 80 mg L-1; 160 mg L-1; 320 mg L-1. O efeito do volume de meio nutritivo sobre o desenvolvimento in vitro de palma foi avaliado por meio do enchimento dos frascos de cultivo (medindo 9 cm de altura x 8 cm de diâmetro da boca x 7 cm de diâmetro de fundo) com os...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Produção de mudas; Opuntia tuna; Protocolo; Clonagem in vitro; Cactácea; Semiárido; Cultura de tecidos.; Micropropagação; Biotecnologia; Planta forrageira; Palma forrageira; Cochonilha.; Opuntia; Tissue culture.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1062965

Producao comercial de mudas de bananeira em laboratorios de cultura de tecidos.
Provedor de dados: 14
Autores: OLIVEIRA, R. P. de; MELO, N. F. de.
Objetiva-se com o trabalho relatar de forma simplificada um protocolo viável para a produção comercial de mudas de bananeira, aplicável a cultivares/ híbridos diplóides, triplóides e tetraplóides em laboratórios de cultura de tecidos.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Producao comercial; Seedling; Commercial production; Banana; Cultura de Tecido; Muda; Micropropagação; Musa; Tissue culture.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/136164

Propagacao: cultura de tecidos do mamoeiro.
Provedor de dados: 14
Autores: NUNES, R. F. de M.
Seminario apresentado sobre a cultura do mamoeiro. Propagacao vegetativa da cultura de tecidos do mamoeiro. Embriogenese somatica. Recuperacao de embriao interespecifico, cultivo do meristema, vantagens e desvantagens da micropropagacao.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Papaya; Meristem; Mamão; Carica Papaya; Cultura de Meristema; Cultura de Tecido; Micropropagação; Propagação Vegetativa; Callus; Tissue culture; Vegetative propagation.
Ano: 1995
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/132699

Programa de pesquisa de acerola a Embrapa Mandioca e Fruticultura.
Provedor de dados: 14
Autores: OLIVEIRA, J. R. P.; SOARES FILHO, W. dos S.; NASCIMENTO, A. S. do; Costa, D. da C.; MATSUURA, F. C. A. U.; GOMES, J. de C.; CARVALHO, J. E. B. de; REINHARDT, D. H. R. C.; OLIVEIRA, R. P. de.
Por sua riqueza em vitamina C e adaptação e ambientes tropicais, a cultura da acerola tem se expandido em varias regiões do pais. Os pomares comerciais brasileiros, porem, apresentam grande desuniformidade entre plantas, no tocante a diversos caracteres de importância agronômica, a exemplo de: produtividade, qualidade de frutos, conformação e vigor da copa. Essa variabilidade genética, todavia, enriquecida pela introdução de variedades promissoras provenientes do exterior, particularmente de Porto Rico e Estados Unidos da América do Norte (Florida e Havai), pode permitir a seleção de genótipos horticulturalmente superiores, possíveis de serem indicados como cultivares adaptadas aos nossos diferentes agro ecossistemas. Com base no exposto, a Embrapa...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Selecao de genotipos; Manejo cultural; Cultura de tecidos; Genotype selection; Cultural management.; Malpighia Punicifolia.; Tissue culture; Vegetative propagation.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/638286

Automacao e racionalizacao na micropropagacao industrial.
Provedor de dados: 14
Autores: FARI, M.; MELO, N. F. de.
Tendencias da mecanizacao na propagacao in vitro em larga escala, automacao das etapas dos metodos tradicionais de cultura de tecidos, novastecnologias baseadas na utilizacao de sistemas de propagacao em meio liquido, desenvolvimento de sistemas de cultivo in vitro alternativos, sistema CLONER: uma nova iniciativa de racionalizacao da bancada de micropropagacao.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Propagacao in vitro; In vitro propagation; Biotecnologia; Cultura de Tecido; Meristema; Micropropagação; Biotechnology; Micropropagation; Tissue culture.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131977

Sergey Fedoroff: A Pioneer of the Neuronal Regeneration. Tribute from the Pan American Association of Anatomy
Provedor de dados: 77
Autores: Losardo,Ricardo Jorge; Cruz Gutiérrez,Rolando; Prates,José Carlos; Moscovici,Mauricio; Rodríguez Torres,Alberto; Arteaga Martínez,Manuel.
Sergey Fedoroff (1925-2012) is considered the father of tissue culture because of his important findings and contributions. He was president of several societies, such as the Pan American Association of Anatomy.
Tipo: Journal article
Palavras-chave: Sergey Fedoroff; Pan American Association of Anatomy; Tissue culture; Neurobiology.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200059

Plant regeneration from mature seed of timothy (Phleum pratense L.) and improvement of regenerating capacity in the progeny
Provedor de dados: 19
Autores: Horikawa, Yoh; Ohi, Hiroyuki; 堀川, 洋; 大井, 弘幸.
Palavras-chave: Phleum pratense L.; Plant Regeneration; Progeny; Tissue culture; チモシー; 植物体再分化; 後代; 組織培養.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1408

Propagation of farinha-seca in vitro
Provedor de dados: 190
Autores: Rossi, Ediana; Sartoretto, Laudete Maria.
The present study aimed to establish protocols for disinfestation, germination of seeds, as well as the induction of in vitro multi sprouting of farinha-seca (Albizia niopoides) using seeds and nodal segments from seedlings germinated in vitro. To evaluate the disinfestations and germination intervals of 0,10, 20 and 30 minutes of soaking seeds in sodium hypochlorite 8% were tested. The evaluations of contamination by fungi and/or bacteria, as well as the germination of seeds were performed 20 days after the test started. The induction of multi sprouting was performed in WPM culture medium supplemented with BAP at concentration of 0.0, 0.5, 2.5 and 5.0 μM and ANA at a concentration of 0.5 μM. Number of sprouts and callus formation were evaluated. The F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disinfestation; Tissue culture; Micropropagation Engenharia Florestal; Genética Vegetal Desinfestação; Cultura de tecidos; Micropropagação.
Ano: 2013
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/361

Simulation of in vitro water deficit for selecting drought-tolerant banana genotypes
Provedor de dados: 86
Autores: Marssaro,Alice Lichs; Morais-Lino,Lucymeire Souza; Cruz,Jailson Lopes; Ledo,Carlos Alberto da Silva; Santos-Serejo,Janay Almeida dos.
Abstract: The objective of this work was to determine a method to simulate water stress in vitro to select drought-tolerant banana (Musa sp.) genotypes. The BRS Tropical and Prata Anã cultivars were grown in vitro in an MS liquid medium containing sucrose and benzylaminopurine (BAP), supplemented with different concentrations of polyethylene glycol (PEG) or sorbitol. The yield stability index of dry matter was evaluated. Cultivation for 30 days in a medium containing 15 g L-1 PEG or 36.4 g L-1 sorbitol is appropriate to simulate water stress in vitro.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa; PEG; Sorbitol; Tissue culture.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201301

ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT
Provedor de dados: 86
Autores: GLORIA,BEATRIZ APPEZZATO DA; VIEIRA,MARIA LUCIA CARNEIRO; DORNELAS,MARCELO CARNIER.
With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001100005

Plant regeneration from protoplast of Brazilian citrus cultivars
Provedor de dados: 86
Autores: GLORIA,FERNANDA JANUZZI MENDES DA; MOURÃO FILHO,FRANCISCO DE ASSIS ALVES; MENDES,BEATRIZ MADALENA JANUZZI.
A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. Somatic embryo formation occurred spontaneously after two subcultures, on modified MT medium supplemented with 500 mg/L of malt extract....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivar improvement; Polyethylene glycol; Rootstock; Tissue culture.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400008

Salt concentrations in culture media for the development of Dipteryx alata in vitro
Provedor de dados: 86
Autores: Araruna,Elequisandra da Costa; Ribeiro-Oliveira,João Paulo; Pereira,Vanderley José; Asmar,Simone Abreu; Melo,Berildo de.
Abstract: The objective of this work was to evaluate how salt concentrations in media used for in vitro cultures affect the early development of “barueiro” (Dipteryx alata) seedlings obtained from the shoot apex. Two different in vitro culture media - Murashige &amp; Skoog (MS) and wood plant medium (WPM) - in different salt concentrations (25, 50, 75, and 100%) were used. At 120 days, the D. alata seedlings in the MS medium, in its original concentration (100%), developed better than in other concentrations or in the WPM. Therefore, the MS medium was considered the most suitable for ensuring in vitro establishment because it provided greater root length (27.65 cm) and number of leaves per plant (26 leaves) than the other medium.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado biome; Culture media; Osmoregulation; Plant growth; Salt concentrations; Tissue culture.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201295

Development of blast resistant somaclones of the upland rice cultivar araguaia
Provedor de dados: 86
Autores: ARAÚJO,LEILA GARCÊS DE; PRABHU,ANNE SITARAMA; FREIRE,ADELSON DE BARROS.
The degree of blast resistance of upland rice (Oryza sativa L.) cultivar Araguaia has decreased over time causing significant yield losses. The major objective of this study was to obtain blast (Pyricularia grisea) resistant somaclones, adapting greenhouse and field selection procedures. Rice blast resistance and agronomic traits were assessed in R2 to R6 generations derived from regenerant plants (R1) from immature panicles of Araguaia. The evaluation and selection procedures include testing of early segregating populations and fixed lines in the advanced generations, under natural field conditions, and artificial inoculations in the greenhouse, with prevalent races IB-1 and IB-9 of P. grisea. Somaclones with both vertical resistance and slow blasting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Pyricularia grisea; Somaclonal variation; Tissue culture.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000200015

Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris
Provedor de dados: 86
Autores: Mello,Marcia Ometto de; Amaral,Antônio Francisco de Campos; Melo,Murilo.
The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fructokinase; Glycolysis; Tissue culture.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2001000900001

Macropropagation and micropropagation of Ziziphus spina-christi
Provedor de dados: 86
Autores: Assareh,Mohammad Hassan; Sardabi,Hossein.
Christ's thorn (Ziziphus spina-christi (L.) Desf.) is a cross-pollinated plant with a wide range of genetic variability in nature and, for this reason, vegetative propagation assumes importance for improvement programs. The objective of this work was to evaluate cutting, T budding and tissue culture methods for this species. Shoots of 22-25 cm length were treated by two culture media and three shoot diameters for cutting trial. The T budding treatments consisted of three and five collection dates in spring and autumn, respectively. Tissue culture nodal segments bearing axillary buds were removed from shoots of mature trees at different seasons. Experiments to determine the best disinfectant chemical, appropriate conditions and materials to prevent phenolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutting; T budding; Tissue culture; Media.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000500006

An efficient protocol for culturing meristems of sorghum hybrids
Provedor de dados: 85
Autores: Sadia¹,B; Josekutty,PC; Potlakayala,SD; Patel,P; Goldman,S; Rudrabhatla,SV.
A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype NC+262, followed by NC+6C21 (31.3 ± 0.8), and NC+6B50 (16 ± 1.7). MS medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Sorghum bicolor; Tissue culture; Genetic improvement; Genotypes.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200010

Laboratory Protocols 2008: Maize Nutrition Quality and Plant Tissue Analysis Laboratory
Provedor de dados: 31
Autores: Gallicia,   L.; Nurit,   E.; Rosales,   A.; Palacios-Rojas,   N.
Tipo: Report
Palavras-chave: Zea mays; Maize; Wheats; Triticum; Transgenic plants; Genetic engineering; Gene transfer; Genetic transformation; DNA hybridization; Phenotypes; Selection; Tissue culture; In vitro culture; Biolistics; Research projects; Methods; Molecular cloning; PCR; Crop Production/Industries; F02; F30.
Ano: 2009
URL: http://purl.umn.edu/56177

Optimization of a protocol for the micropropagation of pineapple
Provedor de dados: 94
Autores: ALMEIDA,WELITON ANTONIO BASTOS DE; SANTANA,GESSIONEI SILVA; RODRIGUEZ,ADRIANA PINHEIRO MARTINELI; COSTA,MARIA ANGÉLICA PEREIRA DE CARVALHO.
The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L.) Merrill) cv. Pérola. Changes in benzylaminopurine (BAP) concentration, type of medium (liquid or solidified) and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%), for three minutes, followed by calcium hypochlorite (2%), for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1), 27 ± 2ºC. After eight weeks,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; Tissue culture; In vitro culture; In vitro clonal propagation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000200005

Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma
Provedor de dados: 94
Autores: Moura,Elisa Ferreira; Motoike,Sérgio Yoshimitsu.
The biotechnological techniques may help solve many problems of guava culture, such as the high perishability of fruits. Somatic embryogenesis can generate highly multiplicative cell cultures and with high regenerative potential, serving as basis for genetic transformation. The aim of this work was to obtain somatic embryogenesis of guava (Psidium guajava L.) cv. Paluma. Immature seeds were used, and they were inoculated in MS environment containing 400 mg L-1 of L-glutamine, 100 mg L-1 myo-inositol, 60 g L-1 sucrose, 100 mg L-1 ascorbic acid and supplemented with different types and concentrations of growth regulators. Embryogenic callus appeared after 37 days of culture in an environment containing 1.0 mg L-1 2.4-D + 2.0 mg L-1 2-ip, in 7% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Growth regulators; Myrtaceae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452009000200027

Advances in passion fruit (Passiflora spp.) propagation
Provedor de dados: 94
Autores: Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Junghans,Tatiana Góes; Jesus,Onildo Nunes de; Miranda,Diego; Otoni,Wagner Campos.
Abstract  The propagation of many passion fruit species (Passiflora spp.) is done by seeds or sexual method, although asexual propagation, such stem cuttings, grafting or tissue culture is also possible and very useful in many cases. In this work, it was presented information on scientific and technological advances in sexual and asexual propagation of passion fruit, considering the different propagation methods and their applications. New demands for research and development activities are also related, given the wide range of passion fruit species and its potential for food, ornamental and medicinal use.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seed germination; Vegetative propagation; Cuttings; Grafting; Tissue culture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000201002

Somatic embryogenesis from zygotic embryos of Euterpe oleracea Mart.
Provedor de dados: 94
Autores: Ledo,Ana da Silva; Lameira,Osmar Alves; Benbadis,Abdellatif Kemaleddine; Menezes,Ilmarina Campos de; Oliveira,Maria do Socorro Padilha de; Medeiros Filho,Sebastião.
The aim of this work was to study the morphogenetic responses of zygotic embryos of açai palm (Euterpe oleracea Mart.) submitted to several conditions of in vitro culture. Several research experiments were conducted, in laboratory, using vegetable material collected from açai palm plants at Embrapa Amazon Oriental, Belém-PA, Brazil. It was possible to verify the expression of a direct, repetitive and no-synchronized model of somatic embryogenesis in mature zygotic embryos cultivated in primary MS medium supplemented with 2,4-D (339.36 muM) and transferred to a secondary MS medium in the presence of NAA (0.537 muM) and 2iP (12.30 muM). The conversion of somatic embryos into seedlings was reached after 210 days with the transfer of the cultures to a third...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Açai palm; Growth regulators; Micropropagation; Tissue culture.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300004

In vitro organogenesis of rangpur lime
Provedor de dados: 94
Autores: Soriano,Leonardo; Tavano,Eveline Carla da Rocha; Behling,Maurel; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi.
Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L-1). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adventitious shoot; Citrus limonia; Tissue culture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452012000200006

Development of globular embryos from the hybridization between 'Pêra Rio' sweet orange and 'Poncã' mandarin
Provedor de dados: 94
Autores: Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de.
This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031

ADVANCES IN GUAVA PROPAGATION
Provedor de dados: 94
Autores: PEREIRA,FERNANDO MENDES PEREIRA; USMAN,MUHAMMAD; MAYER,NEWTON ALEX; NACHTIGAL,JAIR COSTA; MAPHANGA,OSCAR RANNY MBONGENI; WILLEMSE,SALOMIE.
ABSTRACT  Guava (Psidium guajava L.) can be propagated by seed, layering, air layering, grafting (budding or grafting), cuttings (root or shoot) or tissue culture. Propagation by seed is used for rootstock production and for raising populations for screening at early phases in the breeding programs. Vegetative propagation methods are used to clone selected genotypes from these programs and commercial orchards because it perpetuates all characteristics of each cultivar. This review addresses different methods that can be used to propagate guava, the methods commercially adopted and the progress obtained in recent years. There are several propagation technologies available, however, the adoption levels are rather different between producing countries. Needs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psidium guajava; Seeds; Grafting; Cutting; Air layering; Tissue culture.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401002

Selection of somaclonal variants of the cultivar ‘Prata-Anã’ for resistance to Fusarium oxysporum f. sp. cubense race 1
Provedor de dados: 94
Autores: Ferreira,Mileide dos Santos; Moura,Érica Rodrigues de; Lino,Lucymeire Souza Morais; Amorim,Edson Perito; Santos-Serejo,Janay Almeida dos; Haddad,Fernando.
Abstract  The banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ‘Prata-Anã’ resistant to Fusarium wilt by inducing somaclonal variation. ‘Prata-Anã’ stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Tissue culture; Musa sp.; Somaclonal variations; Fusarium wilt.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000600101

ADVANCES IN PEACH, NECTARINE AND PLUM PROPAGATION
Provedor de dados: 94
Autores: MAYER,NEWTON ALEX; BIANCHI,VALMOR JOÃO; FELDBERG,NELSON PIRES; MORINI,STEFANO.
ABSTRACT  Nursery trees of stone fruits (Prunus spp.) are traditionally produced by union of two distinct genotypes - the rootstock and the scion - which, by grafting, form a composite plant that will be maintained throughout of all plant life. In Brazil, the rootstocks are predominantly seed propagated and therefore usually results in heterogeneous trees for vigor and edaphic adaptation. However, with advances in rootstock breeding programs that released cultivars and certification in several countries (notably in Europe), the system will come gradually evolving for vegetative propagation (cuttings and tissue culture) and use of seeds of selected rootstocks with specific characteristics and potted nursery trees production. For scion cultivar propagation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus spp.; Seeds; Cutting; Grafting; Air layering; Tissue culture.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401001

Growth and volatiles in the micropropagation of Santa Maria herb
Provedor de dados: 124
Autores: Carvalho,Alexandre Alves de; Bertolucci,Suzan Kelly Vilela; Silva,Sâmia Torres; Pinto,José Eduardo Brasil Pereira.
ABSTRACT The aims of this study were to establish micropropagation for Chenopodium ambrosioides L. and analysis of volatile constituents in vitro as a function of different salt concentrations of MS medium and sucrose. The apical buds were established on MS medium added with 3% sucrose. The vegetative growth of nodal segments was tested in different salt concentrations of MS basic medium (0.25, 0.50, 1.00, 1.50, and 2.00) and of sucrose (5, 10, 20, 30, and 40 g L-1). Nodal and apical segments were tested in vertical and horizontal orientations. Different volumes of culture medium (20; 35, 50, 65, and 80 mL) were examined in the growth of nodal segments. Plants were acclimatized in three different substrates. The apical segments collected from matrices...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chenopodium ambrosioides L.; Medicinal plant; Tissue culture.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000400624

Artificial light and growth regulators on the in vitro etiolation of Cattleya labiata
Provedor de dados: 124
Autores: Rodrigues,Antonio Anderson de Jesus; Santos,Eder de Oliveira; Takane,Roberto Jun; Carvalho,Ana Cristina Portugal Pinto de.
ABSTRACT The technique of etiolation is widely used in the micropropagation of various plant species. The aim of this study was to evaluate growth and development in shoots of Cattleya labiata grown in vitro, as a function of growth regulators and the presence and absence of light. Stem segments, approximately ± 1.0 cm in height, were inoculated in test tubes containing 15.0 mL of MS culture medium supplemented with different concentrations of BAP (0.0, 2.0 and 4.0 mg L-1) and NAA (0.0, 1.0 and 2.0 mg L-1), and in different growth environments (growth room, darkness and a photoperiod of 16 hours) in a completely randomised design. At 150 days, the following were evaluated: a) number of etiolated shoots; b) number of nodes per etiolated shoot; c) length of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orchidaceae; Tissue culture; Photoperiod; 6-benzylaminupurine; Naphthaleneacetic acid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000200296

Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources
Provedor de dados: 63
Autores: Mello,Marcia Ometto; Dias,Carlos Tadeu Santos; Amaral,Antônio Francisco Campos; Melo,Murilo.
Cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. were used to test plant ability to utilize an alternative to sucrose as carbon source and energy for growth. Glycerol, sorbitol and galactose were the alternative carbon sources tested. Cell suspension cultures established on liquid medium containing sucrose were transferred to culture medium supplemented with sucrose or glycerol, or sorbitol, or galactose as the sole carbon source. Fresh and dry weight increasing and protein content showed marked differences among the different carbon sources used. Sucrose was the best carbon source for all the three plant species tested. Galactose and glycerol promoted slow or no growth of the three studied species....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sucrose; Glycerol; Sorbitol; Galactose; Tissue culture.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000300007

Water deficit in relation to leaf and stem anatomy of Eucalyptus camaldulensis Dehn. shoots cultivated in vitro
Provedor de dados: 63
Autores: Souza,Gustavo Maia; Gonçalves,Antonio Natal; Almeida,Marcílio de.
Shoots of E. camaldulensis seedlings from three distinct provenances were submitted to three different levels of in vitro water deficit induced by the addition of sorbitol in the growth media. Transversal sections from the leaf blades and stems were anatomically analysed using the historesin inclusion technique. Shoots from the different provenances presented distinct responses to the treatments. The Gilbert River provenance was the most sensible. The basic alterations found on the anatomical structure in response to the water deficit were: cell collapse, late tissue differentiation, vascular bundle and epidermis disorganization, and alterations on the mesophyll and epidermis thickness.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus camaldulensis; Water deficit; Anatomy; Tissue culture.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000300030

Somaclonal variation in micropropagated Heliconia bihai cv. Lobster Claw I plantlets (Heliconiaceae)
Provedor de dados: 63
Autores: Rodrigues,Paulo Hercílio Viegas.
The occurrence of somaclonal variation is described in various cultures of agronomic interest. Such variation can be of benefit in the development of new flower varieties. In this study, the occurrence of somaclonal variation in micropropagated changes of Heliconia bihai cv. Lobster Claw I was investigated. Stem apexes were introduced in MS culture media with the addition of 2.5 mg L-1 of benzylaminopure (BAP) and 500 mg L-1 of sodium cefotaxime. After selecting the apex stem, it was sub-cultivated in MS media and supplemented with 4.0 mg L-1 of BAP to induce side buds. To conduct the trial, 2,000 plants were selected and compared with plants originated from rhizomes. To calculate the percentage of the variants, the plant stature, the form and color of...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Tissue culture; Flower growing; Variability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000600017

Optimization of in vitro organogenesis in passion fruit (Passiflora edulis f. flavicarpa)
Provedor de dados: 63
Autores: Trevisan,Flavio; Mendes,Beatriz Madalena Januzzi.
In vitro organogenesis of passion fruit was studied by the induction of adventitious buds from leaf discs in culture media supplemented with benzyladenine (BAP) or thidiazuron (TDZ). To minimize adverse effects of ethylene accumulation on shoot development, silver nitrate (AgNO3) was added to the induction media. Both BAP (0; 2.2; 4.4; 6.6 µmol L-1) and TDZ (0; 1.1; 2.2; 3.4 µmol L-1) were effective in promoting shoot development. Although no significant differences were detected using AgNO3 (23.5 µmol L-1), buds grown in AgNO3-supplemented media were more vigorous. The number of explants with buds obtained using TDZ and AgNO3-supplemented media (5.6) were higher than those obtained using BAP and AgNO3 (3.0). MSM + giberrellic acid (GA3), MSM + coconut...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ethylene; Silver nitrate; Thidiazuron; Tissue culture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400007

In vitro establishment of Heliconia rauliniana (Heliconiaceae)
Provedor de dados: 63
Autores: Rodrigues,Paulo Hercilio Viegas.
The great interest for heliconias by the external market has placed this crop in a prominent position in the flower production chain. The small offer of healthy seedlings, however, the expansion of this activity. The presence of endophytic microorganisms such as Pseudomonas sp. represents an obstacle even for the evolution of heliconia tissue culture studies. Stem apices of Heliconia rauliniana were inoculated and analyzed in vitro to investigate the presence of endophytic microorganisms. An antibiotic analysis was also run for posterior microbiological control. The presence of Pseudomonas sp. and Klebsiella sp. in stem apices confirmed infection. Antibiotics such as chloramphenicol, cefotaxime and the association of both (chloramphenicol + cefotaxime) at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Microbiologic control; Endophytics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100013

In vitro seed germination and seedling development of Annona crassiflora Mart.
Provedor de dados: 63
Autores: Ribeiro,Márcia de Nazaré Oliveira; Pasqual,Moacir; Villa,Fabíola; Pio,Leila Aparecida Salles; Hilhorst,Henk William Maria.
Annona crassiflora Mart known as 'araticum', 'marolo' or 'field araticum' is a typical fruit from the Cerrado biome of Brazil with socio-economic and medicinal importance. Normally, Annona crassiflora is propagated through seeds. However, due to a deep dormancy that the seeds display at dispersion and the difficulty to obtain uniform plants in a short time period, micropropagation may be a feasible alternative. Concentrations of gibberellic acid (GA3) and naphthalene-acetic acid (NAA) and their interactive effects on in vitro seed germination and seedling development of Annona crassiflora were studied. Mature fruits of Annona crassiflora were depulped and the seeds washed in clear water and dried at room temperature. Seed coat was removed and the seeds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Culture medium; Growth regulators.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300017

In vitro sensivity of fig plantlets to gamma rays
Provedor de dados: 63
Autores: Ferreira,Ester Alice; Pasqual,Moacir; Tulmann Neto,Augusto.
Fig breeding programs through conventional methods are rare in many countries, e.g. Brazil, since the wasp Blastophaga psenes, which is responsible for the natural pollination, is not present. For these cases a low cost alternative for the breeding program is the induction of physical mutagenesis by radiation. The sensivity of fig explant buds of different sizes to gamma radiation were evaluated. Fig plantlets "Roxo de Valinhos" already established in vitro were classified by size in 2.5 to 4.5 cm, 5 to 9 7 cm and 8 to 10 cm long, and irradiated with: 10, 20, 30, 40 and 50 Gy doses. After irradiation each plantlet was cut in pieces containing one-bud and transferred to WPM culture medium, according to the bud position: medium and apical. Explants were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Breeding; Tissue culture.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000400017

Acclimatization of 'VR043-43' (Vitis vinifera x Vitis rotundifolia) grapevine rootstock
Provedor de dados: 63
Autores: Machado,Marília Pereira; Biasi,Luiz Antonio; Ritter,Marlice; Ribas,Luciana Lopes Fortes; Zanette,Flávio.
The pre-acclimatization stage can be used to improve micropropagation protocols and increase the yield of produced plants. The influence of sucrose and photon flux density (PFD) levels on the acclimatization of in vitro-grown 'VR043-43' (Vitis vinifera x Vitis rotundifolia) grapevine rootstocks was evaluated. Rooted shoots were obtained from 4-week-old in vitro shoots cultivated in QL (Quoirin and Lepoivre, 1977) culture medium supplemented with 15, 30 and 45 g L-1 of sucrose. The experiment was kept in a 25 ± 2ºC growth room, under 16-h photoperiod and PFD of 18 µmol m-2 s-1 or 43 µmol m-2 s-1. Plants were transferred to an intermittent misting system greenhouse for 10 d followed by 20 d of once-a-day watering routine using a handheld hose. Plant height...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Sucrose; Micropropagation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400007

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla
Provedor de dados: 63
Autores: Alcantara,Giovana Bomfim de; Bespalhok Filho,João Carlos; Quoirin,Marguerite.
The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Agrobacterium tumefaciens; Micropropagation; Tissue culture; Transgenic plant; Woody species.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200016

Slow-grown in vitro conservation of Heliconia champneiana cv. Splash under different light spectra
Provedor de dados: 63
Autores: Rodrigues,Paulo Hercílio Viegas; Arruda,Flávia; Forti,Victor Augusto.
ABSTRACT: In vitro techniques, with the purpose of conserving the genetic resources of plants, are fundamental to the feasibility of establishing germplasm banks and enabling the commercial production of micropropagated plants. The aim of this study was to evaluate the use of different spectra of light in in vitro germplasm conservation by slow grown storage of Heliconia Champneiana cv. Splash. Explants of heliconia in vitro were submitted to the following light treatments: CW (control white), B100 (100 % blue), R100 (100 % red) and R70B30 (70 % red/30 % blue), all with PFD = 25 μmol m2 s–1, maintained in vitro for two time periods, namely P1, 6 weeks and P2, twelve weeks, and were statistically evaluated after each interval with respect to: height, fresh...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Micropropagation; Ornamental plants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000200163

Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro
Provedor de dados: 63
Autores: Borgatto,Fábio; Dias,Carlos Tadeu dos Santos; Amaral,Antônio Francisco Campos; Melo,Murilo.
The chemical composition and vegetative vigor of the donor plant are essential for the satisfactory performance of explants in vitro. In order to test the effect of potassium, calcium and magnesium nutritional status of Chrysanthemum morifolium plants on callogenesis in vitro, pot plants growing in sand were irrigated with nutrient solution containing different levels of potassium (0; 58.5; 117 and 234 mg L-1), calcium (0; 50; 100 e 200 mg L-1) and magnesium (0;12; 48 e 96 mg L-1). After 30 and 45 days, explants (shoot segments and leaf discs) were collected, desinfected and inoculated on MS solid medium supplemented with 0.1 mg L-1 of kinetin and 5.0 mg L-1 of nafthalene acetic acid for callogenesis induction. Callogenesis evaluated as callus fresh weight...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix plant; Mineral nutrition; Tissue culture.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400011

Embryogenic calli induction from nucellar tissue of Citrus cultivars
Provedor de dados: 63
Autores: Mendes-da-Glória,Fernanda Januzzi; Mourão Filho,Francisco de Assis Alves; Demétrio,Clarice Garcia Borges; Mendes,Beatriz Madalena Januzzi.
Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck) produced hard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Tissue culture; Cultivar improvement; Growth regulator; Protoplast.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012

วิธีทางซีรัมวิทยาในการตรวจหา Potato Virus X (PVX) จากพืชที่ได้จากการเพาะเลี้ยงเนื้อเยื่อและหัวพันธุ์มันฝรั่ง
Provedor de dados: 106
Autores: Sumerng Kumthong.
Thesis (M.Sc. in Agriculture)
Tipo: PhysicalObject
Palavras-chave: Potato; Solanum tuberosum; Plant; Seed; Potato Virus X; PVX; Serological method; Detection; Tissue culture; มันฝรั่ง; พืช; หัวพันธุ์มันฝรั่ง; ท่อนพันธุ์; ไวรัส; วิธีทางซีรัมวิทยา; การตรวจเชื้อ; การเพาะเลี้ยงเนื้อเยื่อ.
Ano: 1987
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5474

การขยายพันธุ์กีวีฟรุตพันธุ์บรูโนโดยการเพาะเลี้ยงเนื้อเยื่อ
Provedor de dados: 106
Autores: Sathit Wiyaporn.
Thesis (M.Sc. in Agriculture)
Tipo: PhysicalObject
Palavras-chave: Kiwifruit; Propagation; Actinidia chinensis; Bruno; Tissue culture; กีวีฟรุต; การขยายพันธุ์; พันธุ์บรูโน; การเพาะเลี้ยงเนื้อเยื่อ.
Ano: 1986
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5475

Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)
Provedor de dados: 39
Autores: Ambrósio,Sandra Tereza; Melo,Natoniel Franklin de.
The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60g.L-1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diameter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the absence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ferns; Ornamental plants; Tissue culture.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000400011

Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant
Provedor de dados: 39
Autores: Magioli,Claudia; Mansur,Elisabeth.
Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum melongena L.; Biotechnology; Tissue culture; Genetic transformation; Model plant.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000100013

Resposta rizogênica in vitro de ápices caulinares de mamoeiro ‘Tainung 01’ em diferentes tempos de permanência em meios de indução e regeneração - DOI: 10.4025/actasciagron.v31i4.863
Provedor de dados: 1
Autores: Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV.
Este trabalho teve como objetivo avaliar a resposta rizogênica in vitro de ápices caulinares de plantas jovens de mamoeiro ‘Tainung 01’, em diferentes tempos de permanência em meios de indução e regeneração. O meio de indução empregado foi o MS, suplementado com complementos orgânicos constituídos de sacarose, mio-inositol, tiamina, piridoxina, ácido nicotínico, além de AIB. O meio de regeneração diferiu do meio de indução por ser desprovido de AIB. Os tratamentos consistiram em tempos variáveis de permanência dos explantes nos meios de indução e de regeneração (indução/regeneração): 0/25; 5/20; 10/15; 15/10; 20/5 e 25/0 dias. O delineamento experimental foi inteiramente casualisado, com seis tratamentos e quatro repetições, e cada unidade experimental foi...
Palavras-chave: 5.01.03.04-0    Produção de Mudas Carica papaya; Auxina; Micropropagação; Cultura de tecidos Carica papaya; Auxin; Micropropagation; Tissue culture.
Ano: 2009
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/863

In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume - doi: 10.4025/actasciagron.v34i1.10984
Provedor de dados: 1
Autores: Assis, Kerlley Cristina; Empresa Brasileira de Pesquisa Agropecuária, Embrapa Clima Temperado; Pereira, Flávia Dionísio; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano; Cabral, Juliana Silva Rodrigues; Instituto Federal de Educação, Ciência e Tecnologia Goiano; Silva, Fabiano Guimarães; Instituto Federal de Educação, Ciência e Tecnologia Goiano; Silva, José Waldemar; Universidade Federal de Uberlândia; Santos, Silvia Correa dos; Universidade Federal de Goiás.
Anacardium othonianum Rizz. is a medicinal plant species that is native to the Brazilian savannah. Adult plants are different from other genus members in this ecosystem due to their size, and efforts to locate the plants may lead to their extraction from the savannah and, frequently, plant death. Micropropagation has played a significant role in the propagation and preservation of specimens of several plant species; therefore, this study evaluated the effect of various salt concentrations and culture medium volumes on the in vitro cultivation of A. othonianum Rizz. Trial (I) evaluated two culture media (MS and WPM) and three salt concentrations (100, 50 and 25%) in a completely randomized design. Trial (II) evaluated two culture media and salt...
Tipo: Pesquisa
Palavras-chave: 5.01.03.04-0 cashew; Tissue culture; In vitro propagation Produção Vegetal.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984

The influence of moisture on the in vitro embryo germination and morphogenesis of babassu (Orbignya phalerata Mart.) - doi: 10.4025/actasciagron.v34i4.13429
Provedor de dados: 1
Autores: Silva, Marcus Vinícius Vieira da; Instituto Federal Goiano; Sales, Juliana de Fátima; Instituto Federal Goiano; Silva, Fabiano Guimarães; Instituto Federal Goiano; Neto, Aurélio Rubio; Instituto Federal Goiano; Alberto, Paula Sperotto; Instituto Federal Goiano; Pereira, Flávia Dionísio; IFGoiano - Campus Rio Verde.
The objective of this study was to evaluate the physiological quality of babassu (Orbignya phalerata Mart.) embryos as a function of desiccation. The fruits were placed in a convection oven at 37±2°C for 0, 3, 6, 9, or 12 days. After these periods, the moisture contents of the fruits and seeds were determined. A reduction in the water content occurred more rapidly in the fruits than in the seeds. The decrease in seed moisture content from 12.59% (on the basis of fresh weight) to 4.56% did not affect the percentage of germinated zygotic embryos; however, we did observe a decline in the vigor as the seed moisture content decreased. Embryos with a 4.56% moisture content developed into seedlings of shorter lengths after 30, 60, and 90 days of in vitro growth....
Tipo: Pesquisa Empírica
Palavras-chave: 5.01.03.00-8 Arecaceae; Desiccation tolerance; Tissue culture; Fitotecnia.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13429

Teor e composição química do óleo essencial de Melissa officinalis L. in vitro sob influência do meio de cultura - DOI: 10.4025/actasciagron.v31i2.765
Provedor de dados: 1
Autores: Reis, Érika Soares; IFMG; Pinto, José Eduardo Brasil Pereira; UFLA; Rosado, Luciana Domiciano Silva; UFLA; Corrêa, Ricardo Monteiro; IFMG.
Com o objetivo de avaliar o efeito do meio de cultura no teor e composição química do óleo essencial de Melissa officinalis, foi realizado o presente trabalho, que avaliaram três concentrações do meio de cultura MS. O experimento foi implantado em delineamento inteiramente casualizado, com sete repetições, e cada repetição foi representada por uma amostra de 20 g de plântulas frescas. A extração do óleo foi realizada em aparelho de Clevenger modificado com duração de 1h e 30 min. e a análise química foi realizada por cromatografia gasosa. Observou-se que o teor e a composição química do óleo essencial de M. officinalis foram influenciados pela concentração de sais do meio MS. As plântulas que se desenvolveram nos meios MS e MS/4 apresentaram maior teor de...
Palavras-chave: 5.01.00.00-9 Agronomia Melissa officinalis; Planta medicinal; Óleo essencial; Cultura de tecidos Fitotecnia Melissa officinalis; Medicinal plant; Essential oil; Tissue culture.
Ano: 2009
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/765

Níveis de ácido indol butírico (AIB) no enraizamento in vitro de microestacas de mamoeiro ‘Tainung 01’ - DOI: 10.4025/actasciagron.v32i1.866
Provedor de dados: 1
Autores: Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV.
Este trabalho teve como objetivo avaliar níveis de AIB no enraizamento in vitro de microestacas de mamoeiro (Carica papaya L. ‘Tainung 01’). Ápices caulinares de plantas com 120 dias de idade foram estabelecidos e avaliados em meio MS, suplementado com complementos orgânicos constituídos de sacarose a 87,64 mM, mio-inositol a 555,06 µM, tiamina-HCl a 0,30 µM, piridoxina-HCl a 2,43 µM, ácido nicotínico a 4,06 µM e 0; 0,74; 1,48; 2,96 e 5,92 µM de AIB, em delineamento inteiramente casualizado, com quatro repetições e cinco tubos de ensaio por parcela. Após 30 dias, avaliou-se a percentagem de enraizamento, o crescimento das microestacas, o comprimento da maior folha e a massa de calo. Observou-se que o maior percentual estimado de enraizamento ocorreu com a...
Palavras-chave: 5.01.00.00-9    Agronomia Carica papaya; Auxina; Micropropagação; Cultura de tecidos Fitotecnia Carica papaya; Auxin; Micropropagation; Tissue culture.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/866

Immature tassels as alternative explants in somatic embryogenesis and plant regeneration in south Brazilian maize genotypes - doi: 10.4025/actasciagron.v35i1.15545
Provedor de dados: 1
Autores: Grando, Magali Ferrari; Universidade de Passo Fundo; Varnier, Madelaine Lourdes; Universidade de Passo Fundo; Silva, Marilia Rodrigues de; Universidade de Passo Fundo; Emydgio, Beatriz Marti; Empresa Brasileira de Pesquisa Agropecuária; Pereira, Luiz Ricardo; Empresa Brasileira de Pesquisa Agropecuária; Suzin, Marilei; Universidade de Passo Fundo.
Somatic embryogenesis and in vitro plant regeneration are fundamental processes in the obtainment of transgenic maize plants. Explant, genotype and culture medium are determining factors in these processes. Immature embryo explants and the American Hi-II genotype have been widely employed to acquire genetically modified plants in this species. However, the use of more readily available explants is desired as well as the development of genetic transformation protocols for productive genotypes adapted to local conditions. This study provides an evaluation of immature tassel explants in relation to embryogenic callus production and plant regeneration in South Brazilian maize genotypes for their use in genetic transformation experiments. Immature tassels from...
Tipo: Cultura de tecidso in vitro
Palavras-chave: 5.01.03.00-8 embryogenic callus; Tissue culture; Explants; Zea mays 5.01.03.05-9 embryogenic callus; Tissue culture; Explants; Zea mays.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15545

In vitro germination and disinfestation of sweet cactus (Nopalea cochenillifera (L.) Salm Dyck) - doi: 10.4025/actasciagron.v33i3.6275
Provedor de dados: 1
Autores: Castro, Juliana Pereira de; Universidade Federal da Paraíba; Araújo, Emmanuelle Rodrigues; Universidade Federal da Paraíba - UFPB; Rêgo, Mailson Monteiro do; Universidade Federal da Paraíba - UFPB; Rêgo, Elizanilda Ramalho do; Universidade Federal da Paraíba - UFPB.
This work aimed to promote in vitro seed germination and disinfestation of the sweet cactus. Seeds were submerged in alcohol at 70% for 1 min. and then treated with sodium hypochlorite solution at different concentrations: 0.0; 0.5; 1.0 and 1.5%. After this treatment, the seeds were washed four times in distillated, deionized and autoclaved water. Seeds were inoculated in MS medium with different concentrations of sucrose (0.0, 2.5, 5.0, 7.5 and 10.0 g L-1). The experimental design was completely randomized in a factorial experiment 4 x 5, with five replicates for treatments. Contamination, germination and seedling growth were evaluated. The results of the analysis of variance indicate that there were no significant interactions among factors. There was no...
Palavras-chave: 5.01.00.00-9 Cactaceae; Forage; Tissue culture 5.01.00.00-9 Cactaceae; Forage; Tissue culture.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6275

In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium
Provedor de dados: 1
Autores: Brondani, Gilvano Ebling; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Araujo, Marla Alessandra; Universidade Federal de Lavras; Alcântara, Berenice Kussumoto; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Carvalho, Janice Guedes; Universidade Federal de Lavras; Gonçalves, Antonio Natal; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Almeida, Marcilio de; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP).
The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The...
Tipo: Pesquisa com fatores controlados
Palavras-chave: 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15143

Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342
Provedor de dados: 1
Autores: Alcantara, Giovana Bomfim de; Universidade Federal do Paraná; Dibax, Roberson; Universidade Federal do Paraná; Bespalhok Filho, João Carlos; Universidade Federal do Paraná; Daros, Edelclaiton; Universidade Federal do Paraná.
The development of an efficient regeneration system is crucial for the genetic transformation of sugarcane. The aims of this study were to regenerate plants from somatic embryos and analyze the origin and division pattern of cells during the different embryonic developmental stages of the sugarcane cultivars RB855156 and RB72454. For both cultivars, the best results for embryogenic callus induction were obtained with explant cultures on culture medium containing 13.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by callus culture on solid medium with 4.5 µM 2,4-D. A higher rate of shoot induction was observed in RB855156 with the addition of 8.9 and 17.8 µM benzylaminopurine (BAP); this higher rate was also observed in RB72454 with the addition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.00.00.00-4; 5.01.03.00-8; 5.01.03.05-9; Tissue culture; Somatic embryos; Anatomy; 2 4-D; Poaceae Ciências Agrárias  Fitotecnia  Melhoramento Vegetal.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16342

Fontes de silício na micropropagação de orquídea do grupo Cattleya - doi: 10.4025/actasciagron.v33i3.6281
Provedor de dados: 1
Autores: Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Rodrigues, Filipe Almendagna; Universidade Federal de Lavras; Villa, Fabiola; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras.
O objetivo deste trabalho foi determinar a fonte de silício que proporcione melhor crescimento em plântulas de Cattleya loddigesii. Plântulas oriundas de sementes germinadas in vitro com aproximadamente 1,0 cm de comprimento foram inoculadas em tubos de ensaio contendo 15 mL de meio de cultura Knudson C modificado, acrescido de silicato de sódio (0, 5, 10 e 20 mg L-1) e silicato de potássio (0, 5, 10 e 20 mL L-1), em todas as combinações possíveis. O meio de cultura teve seu pH ajustado para 5,8 &amp;plusmn; 0,1 e solidificado com 5 g L-1 de ágar antes do processo de autoclavagem a 121&amp;deg;C e 1 atm por 20 min. O cultivo foi mantido em sala de crescimento com irradiância de 35 &amp;micro;mol m-2 s-1, temperatura de 25 &amp;plusmn; 1&amp;deg;C e...
Palavras-chave: 5.01.03.00-8 Cattleya loddigesii; Meio de cultura; Orchidaceae; Cultura de tecidos Fitotecnia/Biotecnologia Cattleya loddigesii; Culture medium; Orchidaceae; Tissue culture.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6281

Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
Provedor de dados: 42
Autores: PáDUA,MARLÚCIA S.; PAIVA,LUCIANO V.; LABORY,CLAÚDIA R.G.; ALVES,EDUARDO; STEIN,VANESSA C.
ABSTRACTOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses inE.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryogenic potential; Growth regulators; Tissue culture; Transmission electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401545

Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae)
Provedor de dados: 42
Autores: Braga,Virgínia F.; Mendes,Giselle C.; Oliveira,Raphael T.R.; Soares,Carla Q.G.; Resende,Cristiano F.; Pinto,Leandro C.; Santana,Reinaldo de; Viccini,Lyderson F.; Raposo,Nádia R.B.; Peixoto,Paulo H.P.
This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC50 = 169.3 µg mL-1. HF showed a non-dose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Biodiversity conservation; Biological effects; Natural products.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100014

Micropropagation of Glandularia perakii Cov. et Schn. (Verbenaceae): a native species with ornamental potential
Provedor de dados: 47
Autores: Marino,Concepción; Ponce,María T.; Videla,María E.; Fioretti,Sonia; Cirrincione,Miguel.
Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tissue culture; In vitro propagation; Nodal segment; Glandularia perakii.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100007

Hydrogen peroxide in micropropagation of Lilium: A comparison with a traditional methodology
Provedor de dados: 47
Autores: Curvetto,Néstor; Marinangeli,Pablo; Mockel,Gabriela.
The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H2O2) into the nutrient media as chemical sterilizer. A series of H2O2 concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits in all treatments, though it was higher in the H2O2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical sterilization; Lily; In vitro multiplication; Tissue culture.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300010

Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
Provedor de dados: 48
Autores: GHATPANDE,SATISH; SHAFIQ,SAIYID; SIDDIQUI,M.A.Q.
Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such studies, we examined the expression pattern of vMLC2 in chicken embryos at various stages and analyzed the effect of antisense oligonucleotide-mediated interference of vMLC2 function in cultures of whole embryos. Our results showed vMLC2 to be a specific marker for ventricular chamber throughout chicken embryonic development and antisense vMLC2 treatment of primitive streak stage (stage 4) embryos to produce pronounced dilation of heart tube with severe...
Tipo: Journal article
Palavras-chave: Cardiogenesis; Cardiomyopathy; Myosin light chains; Tissue culture.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009

Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
Provedor de dados: 48
Autores: CAVIEDES,PABLO; CAVIEDES,RAÚL; RAPOPORT,STANLEY I.
Down syndrome is determined by the presence of an extra copy of autosome 21 and is expressed by multiple abnormalities, with mental retardation being the most striking feature. The condition results in altered electrical membrane properties of fetal dorsal root ganglia (DRG) neurons, as in the trisomy 16 fetal mouse, an animal model of the human condition. Cultured trisomic DRG neurons from human and mouse fetuses present faster rates of depolarization and repolarization in the action potential compared to normal controls and a shorter spike duration. Also, trisomy 16 brain and spinal cord tissue exhibit reduced acetylcholine secretion. Therefore, we decided to study Ca2+ currents in cultured DRG neurons from trisomy 16 and age-matched control mice, using...
Tipo: Journal article
Palavras-chave: Trisomy 16; Dorsal root ganglion; Calcium current; Tissue culture; Patch clamp; Down syndrome.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300009

Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells
Provedor de dados: 52
Autores: Gallão,Maria Izabel; Cortelazzo,Ângelo Luiz; Fevereiro,Manuel Pedro Salema; Brito,Edy Sousa de.
Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitin; Elicitation; Peroxidase; PAL; Sugarcane; Tissue culture.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002

Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]
Provedor de dados: 52
Autores: Corrêa,Cynthia Manyra; Oliveira,Graciele Nicolodi de; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plant; Tissue culture; Polymnia sonchifolia; Micropropagation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300005

Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
Provedor de dados: 52
Autores: Carvalho,Milene Alves de Figueiredo; Paiva,Renato; Stein,Vanessa Cristina; Herrera,Raírys Cravo; Porto,Jorge Marcelo Padovani; Vargas,Daiane Peixoto; Alves,Eduardo.
This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passifloraceae; Callogenesis; Electromicroscopy; Optic microscope; Tissue culture.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851

Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
Provedor de dados: 52
Autores: Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz.
Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Organogenesis; Anatomy.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013

Anatomical study of Racosperma (ex-Acacia) mangium tissues cultured in vitro
Provedor de dados: 52
Autores: Takemori,N. K.; Marschner,R.; Quoirin,M.; Bona,C.; Zanette,F.
In order to achieve adventitious shoot regeneration from young tissues of Racosperma mangium, several combinations of growth regulators were tested. Kinetin (KIN) and a -naphthalenacetic acid (NAA), thidiazuron (TDZ) and NAA or indole-3-butyric acid (IBA), 4-CPPU and 2,4-dichlorophenoxyacetic acid (2,4-D) were added to basic medium composed of MS salts, MS vitamins and 3% sucrose. Sections of hypocotyls and cotyledons of young seedlings were cultivated on these media. Calli developed on different media and, after two to three months, some of them regenerated organized structures. Histological studies revealed hypertrophy and hyperplasy of medular and cortical parenchyma cells of hypocotyl and cotyledon parenchyma. Formation of meristematic cells and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-CPPU; Leguminosae; TDZ; Tissue culture.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100008

Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.)
Provedor de dados: 52
Autores: Rojas-Lorz,Laura; Arrieta-Espinoza,Griselda; Valdez-Melara,Marta; Pereira,Luiz Filipe Protasio; Gatica-Arias,Andrés.
Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Silver nitrate; Somatic embryos; Plant growth regulators; Tissue culture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100408

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro
Provedor de dados: 52
Autores: Dibax,Roberson; Quisen,Regina Caetano; Bona,Cleusa; Quoirin,Marguerite.
The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 µM NAA and 4.44 µM BAP. The best results for bud regeneration were obtained on MS and WPM media (57.5 and 55% of calluses formed buds, respectively). Shoot elongation and rooting (80%) were obtained on MS/2 medium (with half-strength salt concentration) with 0.2% activated charcoal. Acclimatization was performed in the growth chamber for 48 h and then the plants were transferred to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adventitious buds; Callogenesis; Organogenesis; Tissue culture; Anatomical study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200009

Indução in vitro de raízes adventícias em explantes de salix (Salix humboldtiana Willdenow)(Salix humboldtiana Willdenow)
Provedor de dados: 52
Autores: Paiva Neto,Vespasiano Borges de; Paiva,Renato; Furtado,Deborah E.
With the objective to investigate the in vitro behavior of salix (Salix humboldtiana Willdenow), leaf and nodal segments were inoculated in WPM medium supplemented with NAA and IBA. In leaf explants, the presence of these growth regulators, isolated or in combination, induced the formation of adventicious roots. Root hairs were only observed when NAA was present. In nodal explants, while the combination of 2,68 µM ANA + 2,46 µM IBA induced bud growth, the other treatments (4,92 µM IBA; 2,68µM ANA + 4,92 µM IBA; 5,37 µM ANA + 4,92 µM IBA; 5,37µM ANA) induced the formation of adventicious roots.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Salix; Adventicious roots; Root hairs.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100009

Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)
Provedor de dados: 117
Autores: Barrueto Cid,L. Pedro; Carvalho,Luiz L.C.B.
The conventional technology for in vitro plant conservation for cassava (Manihot esculenta Crantz) germplasm collections is laborious due to the need for several sub-culturing procedures per year. This practice implies high costs for medium preparation, tissue culture tubes, time-consuming labor, risks of contamination, mislabeling of accession, and the need for large growth chambers. We have developed a new procedure using in vitro cultivated nodal axillary buds treated with different abscisic acid (ABA) concentrations to reduce the time for recycling transplants cultivated in a SP basic nutritive medium. Nodal explants were stored for three months with ABA. Plants were obtained after nodal axillary buds were placed in SP medium without ABA. Results...
Tipo: Journal article
Palavras-chave: Tissue culture; Dormancy.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000300011

Effect of coconut water on growth of olive embryos cultured in vitro
Provedor de dados: 65
Autores: Souza,Rafaeli Aparecida Vieira de; Braga,Francyane Tavares; Setotaw,Tesfahun Alemu; Vieira Neto,João; Azevedo,Patrícia Helena de; Azevedo,Virgínia Helena de; Cançado,Geraldo Magela de Almeida.
The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olea europaea; Culture medium; Complex mixture; Tissue culture; Zygotic embryos.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000200016

Effect of salicylic acid and silver nitrate on rutin production by Hyptis marrubioides cultured in vitro
Provedor de dados: 65
Autores: Pedroso,Rita Cássia Nascimento; Branquinho,Núbia Angélica de Ávila; Hara,Alessandra Cristina Boffino de Almeida Monteiro; Silva,Fabiano Guimarães; Kellner Filho,Luis Claudio; Silva,Marcio Luís Andrade; Cunha,Wilson Roberto; Pauletti,Patricia Mendonça; Januário,Ana Helena.
ABSTRACT:  Hyptis marrubioides (Lamiaceae) is a medicinal plant that is native from Brazilian Cerrado. In vitro propagation techniques make use of elicitors to alter metabolic pathways, affecting how molecules are produced both qualitatively and quantitatively. This research aimed to evaluate how abiotic elicitors salicylic acid (SA) and silver nitrate (SN) at concentrations of 30µM or 60µM influence Hyptis marrubioides seedling growth by two different in vitro culture methods. The rutin content was quantified by HPLC-DAD. Compared to an untreated culture, the H. marrubioides methanolic extracts cultured in MS medium for 10 days followed by culture in MS medium containing SN (30µM) for 20 days had 1.28 times higher rutin content. In a second experiment,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salicylic acid; HPLC-DAD; Tissue culture; Silver nitrate; Elicitation; Plant growth regulators.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200400

Regulators, culture media and types of lights in vitro lavender culture
Provedor de dados: 65
Autores: Oliveira,Rayssa Camargo de; Asmar,Simone Abreu; Silva,Herick Fernando de Jesus; Morais,Tâmara Prado de; Luz,José Magno Queiroz.
ABSTRACT:  Lavender is an aromatic ornamental plant that is used widely in the perfume, pharmaceutical, cosmetical and food industries. That is why it is important to study ways to promote a production of lavender raw material. For this, four experiments were carried out in order to study the germination and initial in vitro development of lavender. In the first part the efficiency of the use of hydrogen peroxide in the pre-treatment of seeds inoculated in culture media with different concentrations of gibberellic acid was evaluated. Besides that, the influence of the use of Growlux lamps and white fluorescent lamps on plantlets cultivated in MS and LS media at salts concentrations of 50 and 100% was evaluated. Finally, the effect of the gibberellic acid,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lavandula angustifolia; Tissue culture; GA3; Polyamines; Lamp.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100405

In vitro establishment of  Comanthera curralensis , "sempre viva" native of Chapada Diamantina - Bahia
Provedor de dados: 65
Autores: Albuquerque,Mara Márcia Sampaio; Brito,Alone Lima; Lima,Andressa Priscila Piancó Santos; Alvim,Bruno Freitas Matos; Santana,José Raniere Ferreira de.
ABSTRACT: The goal of the present study was to evaluate the germination, initial growth, and in vitro co-cultivation of  Comanthera curralensis Moldenke, a "sempre viva" native of the Chapada Diamantina state of Bahia. Full strength (MS) and half-strength MS (MS1/2) growth media supplemented with two different sucrose concentrations (15 and 30g L-1) were tested for germination and initial plant growth. Three different plant densities were tested by in vitro culture (8, 10 and 12 plants per container). MS1/2 medium with 15g L-1 sucrose resulted in a higher percentage of germination and plant growth for the in vitro establishment of C. curralensis. The use of 12 plants per container is indicated for cost reduction in C. curralensis in vitro production.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eriocaulaceae; Tissue culture; Germination; In vitro growth; Co-cultivation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000600991

Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes
Provedor de dados: 65
Autores: Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki.
ABSTRACT:  The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Tissue culture; Growth regulators.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402

Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration
Provedor de dados: 65
Autores: Rocha,Paulo Sérgio Gomes da; Oliveira,Roberto Pedroso de; Scivittaro,Walkyria Bueno.
The aim of this research was to evaluate the use of light emitting diodes (LEDs) instead of white fluorescent lamps as light source and adequate growth-medium sucrose concentration for sugarcane micropropagation (Saccharum officinarum L.). Sugarcane (RB 872552 variety) bud explants were evaluated during the multiplication and rooting phases under controlled growth-room conditions. Different light sources (blue, red and green LEDs; Growlux and white fluorescent lamps) and different medium sucrose concentrations (0; 15; 30 and 45g L-1) were used, maintaining constant light intensity (20µmol m-2 s-1), photoperiod (16h) and temperature (25+2°C). The experiment was a completely randomized design, and treatments were arranged in a 5x4 factorial (five light...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharum officinarum; LED; Light source; Tissue culture; White fluorescent lamp.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000700005

Early selection of elite clones of an ornamental bromeliad in vitro
Provedor de dados: 65
Autores: Manfio,Candida Elisa; Motoike,Sérgio Yoshimitsu; Paula,Cláudio Coelho de; Valente,Magno Sávio; Melo,Cristiane Gamarano.
Orthophytum grossiorum is a typical bromeliad from Atlantic forestry threatened of extinction. The objectives of this research were to select O. grossiorum clones with ornamental values easy to propagate in vitro, and establish in vitro propagation protocols for these clones. The project was developed in three steps: germination and in vitro selection of seedlings responsive to BAP (6-benzylaminopurine), selection of clones with ornamental values, and establishment of protocol for in vitro propagation of the selected clones. In the first step only 18.33% of plantlets germinated in vitro were responsive to BAP. These plantlets were selected and replicated in vitro several times, each replicated plantlet constituting a clone. In the second step these clones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micro-propagation; Tissue culture; Breeding.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000700009

Long-term maintenance of in vitro cultures affects growth and secondary metabolism of St. John's Wort
Provedor de dados: 65
Autores: Figueiró,Adriana de Andrade; Correa,Cynthia Manira; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressant properties. Efficiency of micropropagation is often related to the long term maintenance of tissues in culture, which may alter the secondary metabolism of plants. The objective of this study was to evaluate growth and secondary metabolism of in vitro shoots of H. perforatum on short and long term maintenance of cultures (30 and 100 days). The effect of BA and NAA supplementation was evaluated during 30 days of culture. Adventitious shoots were cultivated on MS medium supplemented with 4.4mM BA alone or in combination with 0.05mM NAA for 30 days. A hormone-free medium was used as control. Shoots cultivated for 100 days were maintained in presence of 4.4mM BA....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Hypericum perforatum; Hypericin; Phenolic compounds.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000010

Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings
Provedor de dados: 65
Autores: Silva,Luciano Coutinho; Paiva,Renato; Vargas,Daiane Peixoto; Silva,Diogo Pedrosa Corrêa da; Barbosa,Sandro; Herrera,Raírys Cravo.
Byrsonima intermedia A. Juss. é uma planta medicinal e frutífera do Cerrado, cuja propagação convencional é difícil, devido à presença do endocarpo extremamente lignificado. Embora o hipoclorito de sódio (NaOCl) seja amplamente utilizado para a desinfestação superficial, existem poucos relatos de seus efeitos sobre o crescimento do explante. Neste trabalho, o objetivo foi estudar o efeito de diferentes pHs e períodos de exposição de sementes de B. intermedia em uma solução de NaOCl. As sementes foram submetidas a diferentes tempos de exposição (1, 5 e 10 minutos) numa solução de NaOCl com diferentes pHs (5, 7, 10 e 12) e, após o tratamento com hipoclorito de sódio, os embriões foram inoculados em meio WPM com a concentração de 50% de sais sem sacarose,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Native plant; Tissue culture; PH; NaOCl.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400674

Callus induction in leaf segments of Croton urucurana Baill
Provedor de dados: 64
Autores: Lima,Ednabel Caracas; Paiva,Renato; Nogueira,Raírys Cravo; Soares,Fernanda Pereira; Emrich,Eduardo Bucsam; Silva,Álvaro Augusto Naves.
Croton urucurana Baill., a species belonging to the family Euphorbiaceae, can be useful in the recovery of degraded riparian areas. In the traditional medicine, it is widely used as cicatrizant and in the treatment of rheumatism. However, its seeds present dormancy and low viability, making the propagation of this species a challenge. With the objective of establishing an alternative route for the propagation, the effect of different concentrations of 2,4-D (2,4- dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) or TDZ (thidiazuron) and of NAA (1-naphthaleneacetic acid) with BAP and their possible combinations were evaluated for callus induction in leaf segments. Callus fresh mass was evaluated forty-five days after inoculation. The isolated use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Calogenesis; Tissue culture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100002

In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight &amp;amp; Arn.) Verdc.
Provedor de dados: 64
Autores: Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes.
With the objective to promote in vitro callus induction, cotyledon and hypocotyl segments of "perennial soybean" (Glycine wightii (Wight &amp;amp; Arn.) Verdc.) were inoculated in basal medium MS supplemented with sucrose (1.5 e 3%) and 0.8% agar and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin). The explants were maintained in a dark growth room at 28ºC. The best callus induction was observed in explants (cotyledon and hypocotyl) maintained in medium containing the combination of 2,4-D (1 mg.L-1), kinetin (0.1 mg.L-1) and 3% sucrose. To promote callus subculture, the MS medium was supplemented with different combinations of 2,4-D (0.5 to 4.0 mg.L-1), with or without kinetin (0.1 mg.L-1) and sucrose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine wightii; Leguminosae; Callogenesis; Tissue culture.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000600011

In vitro morphogenesis of Syngonanthus mucugensis Giul: subsp. mucugensis
Provedor de dados: 64
Autores: Lima-Brito,Alone; Resende,Sheila Vitória; Lima,Carolina Oliveira de Cerqueira; Alvim,Bruno Matos; Carneiro,Claudia Elena; Santana,José Raniere Ferreira de.
Syngonanthus mucugensis Giul. subsp. mucugensis is an herbaceous plant with significant economic value in the ornamental dry flower business. The restricted occurrence of the municipality Mucugê-BA, Brazil, exclusively associated with extractive exploitation, has considered this species as endangered. The objective of this work was to evaluate the organogenic potential of three different types of S. mucugensis subsp. mucugensis explants to promote the development of an alternative method to the propagation of the genetic resources of this important plant. The morphogenetic capacities of the leaf, stem and root this species was tested using Murashige and Skoog culture medium at half salt concentration and different concentrations of growth of regulators...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Organogenesis; Micropropagation; Straw-flowers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300010

In vitro propagation of Campomanesia rufa: An endangered fruit species
Provedor de dados: 64
Autores: Sant’Ana,Cecília Ramos de Oliveira; Paiva,Renato; Reis,Michele Valquíria dos; Silva,Diogo Pedrosa Corrêa da; Silva,Luciano Coutinho.
ABSTRACT The Campomanesia rufa is a fruitful species native from Brazil considered as endangered by the IUC (International Union for Conservation of Nature) and low information regarding its propagation is available. In this context, the aim of the present study was to develop in vitro germination, micropropagation and callogenesis protocols for the species. For in vitro germination, seeds were inoculated in MS medium supplemented with GA3 (gibberellic acid) and for shoot induction, the medium was supplemented with three different cytokinins BA (benzyladenine), BAP (6-Benzylaminopurine) or TDZ (Thidiazuron). For shoot growth, culture medium containing BA, BAP, TDZ were maintained under white fluorescent lamps and blue:red light-emitting diodes (LED). GA3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Micropropagation; Myrtaceae; Casaqueira.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000400372

Culture media for the multiplication of wild Manihot species
Provedor de dados: 64
Autores: Sá,Jucieny Ferreira de; Sampaio,Emília dos Santos; Mendes,Maria Inês de Souza; Santos,Karen Cristina Fialho dos; Sousa,Antônio da Silva; Ledo,Carlos Alberto da Silva.
ABSTRACT The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Wild parental; Nutritional media; Tissue culture; Micropropagation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600598

Sealing and explant types on the mangaba micropropagation
Provedor de dados: 64
Autores: Sá,Aline de Jesus; Lédo,Ana da Silva; Lédo,Carlos Alberto da Silva; Pasqual,Moacir; Silva,Ana Veruska Cruz da; Silva Junior,Josué Francisco da.
In micropropagation, especially for mangaba tree botanical variety of Northeastern Brazil, limiting aspects such as ethylene accumulation in the cultivation flask and loss of vigor in subcultures have been observed. This study was aimed at assessing the technical and scientific knowledge of the in vitro propagation of botanical mangaba tree variety and at improving the micropropagation protocol, establishing the in vitro cultivation time, the best type of flask sealing and explant at different micropropagation stages. For the establishment phase and for the first and second subcultures, the MS medium with 3% sucrose and 0.6% agar, supplemented with 1 mg L-1 IAA and 1 mg L-1 BA was used. Evaluations were performed at 30, 50 and 65 days of in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hancornia speciosa; Tissue culture; In vitro ethylene; Nodal segments.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400004

Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
Provedor de dados: 64
Autores: Pio,Leila Aparecida Salles; Ramos,José Darlan; Pasqual,Moacir; Santos,Flavia Carvalho; Junqueira,Keize Pereira.
The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Palinology; Tissue culture.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016