Consulta: subjectFacets:"Transgenic plants"
Registros recuperados: 39
Data/hora: 06/06/2024 00:35:59

Transmisibilidad de aislamiento del Citrus Tristeza Virus (CTV) por áfidos y evaluación de la resistencia en cítricos tranformados con genes del CTV
Provedor de dados: 32
Autores: Loeza Kuk, Emiliano.
La sintomatología en árboles infectados por el Citrus Tristeza Virus (CTV) es  influenciada por factores como la población de variantes de secuencia presentes.  Esta población es modificada en la transmisión por áfidos y la evidencia sugiere  que la interacción del CTV con Toxoptera citricida, incrementa la frecuencia de los  aislamientos severos. Se estudió una huerta en Yucatán con una incidencia inicial  de 11 árboles positivos a CTV y con tres haplotipos de aislamientos no severos,  se caracterizaron los aislamientos presentes en los nuevos árboles positivos y de  manera simultánea los presentes en el vector predominante (T. citricida). Después  de tres años, la incidencia aumentó a 75 árboles positivos, los nuevos positivos  generalmente fueron del...
Tipo: Tesis
Palavras-chave: Transmisión por áfidos; SSCP; Muerte súbita de los cítricos; Plantas transgénicas; Inoculación; PCR tiempo real Aphid transmission; SSCP; Citrus sudden death; Transgenic plants; Challenging; Real-time PCR.
Ano: 2012
URL: http://hdl.handle.net/10521/1065

Transmisibilidad de aislamiento del Citrus Tristeza Virus (CTV) por áfidos y evaluación de la resistencia en cítricos tranformados con genes del CTV
Provedor de dados: 32
Autores: Loeza Kuk, Emiliano.
La sintomatología en árboles infectados por el Citrus Tristeza Virus (CTV) es  influenciada por factores como la población de variantes de secuencia presentes.  Esta población es modificada en la transmisión por áfidos y la evidencia sugiere  que la interacción del CTV con Toxoptera citricida, incrementa la frecuencia de los  aislamientos severos. Se estudió una huerta en Yucatán con una incidencia inicial  de 11 árboles positivos a CTV y con tres haplotipos de aislamientos no severos,  se caracterizaron los aislamientos presentes en los nuevos árboles positivos y de  manera simultánea los presentes en el vector predominante (T. citricida). Después  de tres años, la incidencia aumentó a 75 árboles positivos, los nuevos positivos  generalmente fueron del...
Tipo: Tesis
Palavras-chave: Transmisión por áfidos; SSCP; Muerte súbita de los cítricos; Plantas transgénicas; Inoculación; PCR tiempo real; Doctorado; Fitopatología; Aphid transmission; Citrus sudden death; Transgenic plants; Challenging; Real-time PCR.
Ano: 2008
URL: http://hdl.handle.net/10521/1399

Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass
Provedor de dados: 69
Autores: Lee,Ki-Won; Choi,Gi Jun; Kim,Ki-Yong; Yoon,Sei Hyung; Ji,Hee Chung; Park,Hyung Soo; Lim,Young Chul; Lee,Sang-Hoon.
In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation effectiveness and the expression of the GUS gene were investigated. The highest transformation effectiveness (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the...
Tipo: Journal article
Palavras-chave: Agrobacterium; Forage crop; Italian ryegrass; Transgenic plants.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008

Utilization of genes encoding osmoprotectants in transgenic plants for enhanced abiotic stress tolerance
Provedor de dados: 69
Autores: Khan,Mohammad Sayyar; Ahmad,Dawood; Khan,Muhammad Adil.
Global agriculture in the context of growing and expanding populations is under huge pressure to provide increased food, feed, and fiber. The recent phenomenon of climate change has further added fuel to the fire. It has been practically established now that the global temperature has been on the increase with associated fluctuations in annual rainfall regimes, and the resultant drought and flood events and increasing soil and water salinization. These challenges would be met with the introduction and utilization of new technologies coupled with conventional approaches. In recent years, transgenic technology has been proved very effective in terms of production of improved varieties of crop plants, resistant to biotic stresses. The abiotic stresses such as...
Tipo: Journal article
Palavras-chave: Osmoprotectants; Salt stress; Tolerance; Transgenic plants.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400001

Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
Provedor de dados: 69
Autores: Ghaffar Shahriari,Amir; Bagheri,Abdolreza; Bassami,Mohammad Reza; Malekzadeh-Shafaroudi,Saeid; Afsharifar,Alireza; Niazi,Ali.
Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant expression binary vector pBI121, containing the genes encoding Hemagglutinin-Neuraminidase (HN) and Fusion (F) epitopes of Newcastle Disease Virus (NDV) under the control of CaMV35S promoter and NOS terminator was constructed and introduced into the tobacco ( Nicotiana tabacum) plant by Agrobacterium-mediated transformation. Results: Putative transgenic plants were screened in a selection medium containing 50 mg/L...
Tipo: Journal article
Palavras-chave: Agrobacterium tumefaciens; ELISA; Recombinant vaccine; Transgenic plants.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400006

Introduction of antifungal genes in sunflower via Agrobacterium
Provedor de dados: 69
Autores: Radonic,Laura Mabel; Zimmermann,Julián Marcos; Zavallo,Diego; López,Nilda; López Bilbao,Marisa.
There is evidence that overexpression of transgenes codifying antifungal proteins may confer protection to pathogen attack, and that this protection is increased due to the synergic effect of the expression of two or more genes. On the other hand it is well known that sunflower is a recalcitrant specie, highly difficult to be genetically transformed. In this context, the final aim of this project was to obtain sunflower plants expressing at least two antifungal genes, avoiding sequential transformation. The antifungal genes used encode for two enzymes that degrade the fungal wall (glucanase and chitinase), an osmotin and a ribosome inhibitor protein. Two types of transformation vectors were used: a more traditional system with a double cassette and a novel...
Tipo: Journal article
Palavras-chave: Antifungal genes; Double expression cassettes; Polyprotein cassettes; Sunflower; Transgenic plants.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500008

Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants
Provedor de dados: 74
Autores: Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena.
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015

Effect of soybean ureases on seed germination and plant development
Provedor de dados: 74
Autores: Rechenmacher,Ciliana; Wiebke-Strohm,Beatriz; Oliveira-Busatto,Luisa A. de; Polacco,Joseph C.; Carlini,Célia R.; Bodanese-Zanettini,Maria H.
Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5-co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant development; Functional study; Transgenic plants; Mutants; Urease.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200209

BRS 333RR: cultivar de soja transgênica.
Provedor de dados: 14
Autores: PEREIRA, M. J. Z.; KLEPKER, D.; MOREIRA, J. U. V.
A BRS 333RR é uma cultivar de soja transgênica com alto potencial produtivo, apresenta boa ramificação de plantas e resistência às principais doençcas da soja. A estabilidade na altura de plantas proporciona sua utilização em áreas de baixas latitudes. Excelente potencial produtivo. Grupo de maturidade. Região edafoclimática de adaptação. Reação a doenças. Características. Semeadura.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Variedade; Planta transgênica; Soybeans; Cultivars; Transgenic plants.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/954560

Refúgio: preservar a eficiência da soja Bt está em suas mãos.
Provedor de dados: 14
Autores: ROGGIA, S.; SOSA-GOMEZ, D. R.; BUENO, A. de F.; HIROSE, E.; CORRÊA-FERREIRA, B. S.; HOFFMANN-CAMPO, C. B.; GAZZONI, D. L.; PITTA, R. M.
Manejo responsável. Como a soja Bt atua. Entendendo o refúgio. Modelo de posicionamento da área de refúgio na propriedade.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Variedade; Planta transgênica; Soybeans; Transgenic plants; Cultivars.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1043671

BRS 388RR: estabilidade com alta produtividade.
Provedor de dados: 14
Autores: CARNEIRO, G. E. de S.; PIPOLO, A. E.; MELO, C. L. P. de; LIMA, D. de; FOLONI, J. S. S.; MIRANDA, L. C.; PETEK, M. R.; BORGES, R. de S.; GOMIDE, F. B.; DALBOSCO, M.; DENGLER, R. U.
Estabilidade: a BRS 388RR é uma soja precoce com bom desempenho em diferentes épocas de semeadura e ambientes de produção. Apresenta tipo de crescimento indeterminado, arquitetura moderna e alta produtividade com estabilidade. Grupo de maturidade relativa. Regiões edafoclimáticas de adaptação. Produtividade. Sanidade.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Variedade; Planta transgênica; Soybeans; Cultivars; Transgenic plants.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1003665

CULTIVARES de soja BRS 294RR e BRS 295RR transgênicas: precoces e produtivas.
Provedor de dados: 14
Área de indicação. Caracterísiticas.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Variedade; Planta transgênica; Soybeans; Plant breeding; Varieties; Cultivars; Transgenic plants.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/573810

Avaliação dos riscos ambientais e alimentares do trigo geneticamente modificado - Método GMP-RAM para avaliação caso a caso de plantas geneticamente modificadas.
Provedor de dados: 14
Autores: CREMONEZI, S. M. N.; JESUS-HITZSCHKY, K. R. E.; LIMA, D. U.
bitstream/item/125267/1/2007FD05.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: OGM; Planta transgênica; Impacto ambiental; Trigo; Transgenic plants; Risk assessment; Wheat.
Ano: 2007
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/15993

BRS 334RR: cultivar de soja transgênica: alta produtividade com precocidade.
Provedor de dados: 14
Autores: CARNEIRO, G. E. de S.; PIPOLO, A. E.; MELO, C. L. P. de.
Alta produtividade com precocidade! BRS 334RR: grupo de maturidade, regioes edafoclimáticas de adaptação, época de semeadura, reação a doenças, características médias.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Variedade; Planta transgênica; Precocidade vegetal; Soybeans; Early development; Transgenic plants.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/921734

Soja BRS 413RR: precocidade com alta produtividade.
Provedor de dados: 14
Autores: MELO, C. L. P. de; PIPOLO, A. E.; MOREIRA, J. U. V.; LIMA, D. de; FOLONI, J. S. S.; MIRANDA, L. C.; PETEK, M. R.; BORGES, R. de S.; GOMIDE, F. B.; DALBOSCO, M.; DENGLER, R. U.
BRS 413RR: soja precoce com estabilidade e sanidade. Grupo de maturidade relativa; Regiões edafoclimáticas de adaptação; ciclo, acamamento e densidade de plantas; características; Época de semeadura; Reação a doenças.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Planta transgênica; Variedade; Soybeans; Transgenic plants; Cultivars.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1052529

BRS 360RR: a soja transgênica que você esperava!
Provedor de dados: 14
Autores: CARNEIRO, G. E. de S.; PIPOLO, A. E.; MELO, C. L. P. de.
BRS 360RR: semeadura antecipada, produtividade, precocidade, crescimento indeterminado. Grupo de maturidade relativa 6.2; Região edafoclimática de adaptação; Época de semeadura; Reação à doenças; Densidade de semeadura.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Planta transgênica; Variedade; Soybeans; Transgenic plants; Cultivars.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/925922

Análise de sensibilidade do modelo de Caprio para simulação de evolução de resistência de pragas a toxinas BT.
Provedor de dados: 14
Autores: MAIA, A. de H. N.; DOURADO NETO, D.
bitstream/item/12262/1/documentos_76.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Modelo de Caprio; Praga de planta; Resistência a pesticida; Modelo de simulação; Planta transgênica; Plant pests; Pesticide resistance; Simulation models; Transgenic plants.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/83084

BRSMG 780Frr: cultivar de soja transgênica.
Provedor de dados: 14
Autores: FRONZA, V.; ARANTES, N. E.; ZITO, R. K.
A brsmg 780rr é sua nova opção em soja transgênica para o manejo da ferrugem asiática. Alta produtividade e resistência moderada à ferrugem asiática. Grupo de maturidade. Regiões edafoclimáticas de adaptação (REC 303 - MG Triângulo Mineiro e Alto Paranaíba). Reação a doenças. Características médias. População de plantas e ciclo em solos férteis. Manejo da ferrugem asiática.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Planta transgênica; Variedade resistente; Soybeans; Transgenic plants; Disease resistance.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/922174

Biotecnologia e o melhoramento genético de plantas.
Provedor de dados: 14
Autores: CALVO, E. S.
Biotecnologia e o melhoramento genético de plantas. Mas o que é engenharia genética? Como assim? Como é o DNA? Mas como desenvolver novas variedade de plantas via engenharia genética? Como é feita a transformação? Transformação via agrobactéria e regeneração de plantas por organogênese. Como é feita regeneração de plantas num tubo de ensaio? Transformação com o revólver de genes. De que outra maneira a biotecnologia pode auliar no desenvolvimento de novas variedades? Regeneração de plantas de soja via embriogênese somática. Mas como os genes são identificados? O que é um organismo mutante? Como criar um mutante? Existem riscos associados com o uso de plantas transgênicas?
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Soja; Planta transgênica; Biotecnologia; Soybeans; Transgenic plants.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/460384

BRSGO 7460RR: cultivar de soja transgênica.
Provedor de dados: 14
Autores: MELLO FILHO, O. L. de; NUNES JÚNIOR, J.; ZITO, R. K.; MEYER, M. C.; PIMENTA, C. B.
bitstream/item/57492/1/BRSGO7460RR.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Planta transgênica; Variedade resistente; Soybeans; Disease resistance; Transgenic plants.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/922180

BRS 399RR: soja precoce e resistente a nematóides.
Provedor de dados: 14
Autores: PIPOLO, A. E.; MELO, C. L. P. de; CARNEIRO, G. E. de S.; LIMA, D. de; FOLONI, J. S. S.; MIRANDA, L. C.; PETEK, M. R.; BORGES, R. de S.; GOMIDE, F. B.; DALBOSCO, M; DENGLER, R. U.
BRS 399RR: soja precoce e resistente a nematóides, grupo de maturidade relativa: 6.0 ; Precocidade; Sanidade; Regiões Edafoclimáticas de Adaptação (REC); Época de semeadura; Ciclo, Acamamento e Densidade de plantas; Características gerais; Reação a doenças.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja transgênica; Soja; Nematóide; Planta transgênica; Soybeans; Nematoda; Transgenic plants.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1030662

BRS 359RR: soja precoce para semeadura antecipada!
Provedor de dados: 14
Autores: CARNEIRO, G. E. de S.; PIPOLO, A. E.; MELO, C. L. P. de; MIRANDA, L. C.; PETEK, M. R.; LIMA, D. de; GOMIDE, F. B.; DALBOSCO, M.; DENGLER, R. U.
A BRS 359RR é a soja transgênica precoce, que aliada à semeadura antecipada, favorece a safrinha de milho na melhor época. Possui crescimento indeterminado e alto potencial produtivo, inclusive com bom desempenho em áreas com altitudes acima de 600 metros. Grupo de maturidade relativa. Região edafoclimática de adaptação. Época de semeadura. Reação a doenças. Densidade de semeadura. Características.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Soja; Planta transgênica; Variedade; Soybeans; Transgenic plants.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/943452

Genes are gems:reporting agri-biotechnology
Provedor de dados: 30
Autores: R.L.
Palavras-chave: Biotechnology; Transgenics; Genes; DNA; Mycotoxins; Chickpeas; Transgenic plants; Genetic markers; Genetic engineering; Genomes.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4146

DREB1A allows for more water uptake in groundnut by a large modification in the root/shoot ratio under water deficit
Provedor de dados: 30
Autores: Vadez, V.
Palavras-chave: Transgenics; Evapotranspiration; Drought stress; Genotypes; Nutrient uptake; Transgenic plants; Transpiration (animals); Harvest index; Transcription; Transcription factors.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3355

Biological activity of lectins from grain legumes and garlic against the legume pod borer, Helicoverpa armigera
Provedor de dados: 30
Autores: Arora, R.,( Richa Arora ).
Palavras-chave: Garlic; Pigeonpeas; Proteins; Transgenics; Transgenic plants; Chickpeas; Genes; Enzymes; Animal developmental stages; Genetic transformation.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3230

Host plant resistance to insects in sorghum: present status and need for future research
Provedor de dados: 30
Autores: Sharma, H.C.
Palavras-chave: Genes; Sorghum; Transgenics; Cytoplasm; Transgenic plants; Hybrids; Esters; Gene expression; Biochemical compounds; Phenotypes.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3250

Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum
Provedor de dados: 30
Autores: Girijashankar, V.
Palavras-chave: Somatic embryos; Transgenics; Somatic embryogenesis; Auxins; Regeneration; Protocols; Sorghum; Cytokinins; Embryonic development; Transgenic plants.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3300

Transformation and Regeneration of Groundnut, and Utilization of Viral Genes to Induce Resistance to Virus Diseases
Provedor de dados: 30
Autores: D.V.R.
Palavras-chave: Genes; RNA; Transgenics; Protoplasts; Groundnuts; Transgenic plants; Biotechnology; Regeneration; Genomes; Nucleotides.
Ano: 1992
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4105

A simple and reliable method for the screening of transgenic tobacco plants
Provedor de dados: 86
Autores: Freitas-Astua,Juliana; Astua-Monge,Gustavo; Polston,Jane Elisabeth; Hiebert,Ernest.
Even though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still timeconsuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were seen on the leaves of transformed plants. This reaction occurred regardless of age of the tested plants, and the method has proven to be simple, fast, non-destructive, relatively cheap, and reliable. These results were comparable to those obtained by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kanamycin; Genetically modified organisms; Transgenic plants; Testing.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700015

Bioassay for detection of transgenic soybean seeds tolerant to glyphosate
Provedor de dados: 86
Autores: Torres,Antonio Carlos; Nascimento,Warley Marco; Paiva,Sonia Alessandra Vasconcelos; Aragão,Fernando Antonio Souza de.
Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 muM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Herbicide resistance; Transgenic plants; Biosynthesis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000900005

Mowing Associated to Chemical Control for Glyphosate-Resistant Cotton Stalk Destruction
Provedor de dados: 87
Autores: BRAZ,G.B.P.; ANDRADE JR.,E.R.; NICOLAI,M.; LOPEZ OVEJERO,R.F.; CAVENAGHI,A.L.; OLIVEIRA JR.,R.S.; CHRISTOFFOLETI,P.J.; PEREIRA,A.T.A.; CONSTANTIN,J.; GUIMARÃES,S.C.
ABSTRACT: Post-harvest cotton stalk control is mandatory in many cotton producing countries, and the major methods used for this practice are mechanical and chemical (glyphosate and 2,4-D as the most usual herbicides applied), or a combination of both. However, the adoption of glyphosate-resistant cotton varieties by growers have required the development of alternative chemical treatments. Six trials were conducted to evaluate systems combining mowing and chemical control of glyphosate-resistant cotton stalks. Experiments conducted in 2014 were installed in randomized complete block design with nineteen treatments and four replicates. In 2015, the experiments were also conducted in randomized complete block design with eleven treatments and four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crop residues; Fallow management; Herbicides; Transgenic plants.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100260

Edaphic Entomofauna Variation Depending on Glyphosate Application in Roundup Ready Soybean Crops
Provedor de dados: 87
Autores: PEREIRA,J.L.; ARAÚJO,T.A.; RODRIGUES-SILVA,N.; SILVA,A.A.; PICANÇO,M.C.
ABSTRACT: Currently, biomonitoring is a methodology used to verify the environmental impact of new technologies in the agricultural environment, highlighting edaphic entomofauna that is traditionally used as a bioindicator in this sort of research. Therefore, the objective of this study was to investigate the edaphic entomofauna variation depending on glyphosate application in Roundup Ready soybeans. The experiment was carried out in Coimbra, MG during the 2007/2008 and 2008/2009 cropping seasons. The experimental design was a randomized block design with five replications. The treatments were: non-transgenic soybean with mechanical weeding of weeds; transgenic soybean with mechanical weeding of weeds; transgenic soybean with one glyphosate application and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Bioindicators; Transgenic plants; Herbicide.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100307

Laboratory Protocols 2008: Maize Nutrition Quality and Plant Tissue Analysis Laboratory
Provedor de dados: 31
Autores: Gallicia,   L.; Nurit,   E.; Rosales,   A.; Palacios-Rojas,   N.
Tipo: Report
Palavras-chave: Zea mays; Maize; Wheats; Triticum; Transgenic plants; Genetic engineering; Gene transfer; Genetic transformation; DNA hybridization; Phenotypes; Selection; Tissue culture; In vitro culture; Biolistics; Research projects; Methods; Molecular cloning; PCR; Crop Production/Industries; F02; F30.
Ano: 2009
URL: http://purl.umn.edu/56177

Incorporating Collateral Information Using an Adaptive Management Framework for the Regulation of Transgenic Crops
Provedor de dados: 31
Autores: Linacre,   Nicholas A.; Burgman,   Mark A.; Ades,   Peter K.; Stewart-Oaten,   Allen.
A lack of data often makes biological management decisions difficult and has been an area of contention in the debate over the approval of transgenic crops. Our knowledge of natural systems is limited and our ability to gain additional information, quickly and effectively, is often handicapped by statistical complexity. To adequately cope with this requires new approaches and models that integrate decision-making and management. This paper describes one possible approach to the integration of decision-making and management, which may have application for the regulatory approval of transgenic crops. In many situations countries wishing to approve transgenic crops will have limited data on the environmental performance of the crop. The approach outlined in...
Tipo: Working or Discussion Paper
Palavras-chave: Bayesian estimation; Collateral data; Inference; GMO; Transgenic plants; Bayesian theory; Biotechnology; Biosafety; Regulatory systems; Crop Production/Industries.
Ano: 2005
URL: http://purl.umn.edu/59233

In vitro organogenesis and genetic transformation of mandarin cultivars
Provedor de dados: 94
Autores: Soriano,Leonardo; Tavano,Eveline Carla da Rocha; Correa,Marcelo Favaretto; Harakava,Ricardo; Mendes,Beatriz Madalena Januzzi; Mourão Filho,Francisco de Assis Alves.
Abstract  The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial peptide; Cultivar improvement; Cytokinin; Huanglongbing; Transgenic plants.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200802

Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton
Provedor de dados: 48
Autores: Muzaffar,Adnan; Kiani,Sarfraz; Khan,Muhammad Azmat Ullah; Rao,Abdul Qayyum; Ali,Arfan; Awan,Mudassar Fareed; Iqbal,Adnan; Nasir,Idrees Ahmad; Shahid,Ahmad Ali; Husnain,Tayyab.
BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed...
Tipo: Journal article
Palavras-chave: Chloroplast transient peptide; Cry1Ac; Cry2A; Bt; CTP; Cry genes; Endotoxins; GUS; Transgenic plants.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100014

Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
Provedor de dados: 55
Autores: Marcondes,Jackson; Hansen,Ekkehard.
The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety "Vitória de Verão" genetically modified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edible vaccine; Hepatitis B; Oral immunization; Recombinant antigen; Transgenic plants.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600004

Metabolism and root exudation of organic acid anions under aluminium stress
Provedor de dados: 61
Autores: Mariano,Eduardo D.; Jorge,Renato A.; Keltjens,Willem G.; Menossi,Marcelo.
Numerous plant species can release organic acid anions (OA) from their roots in response to toxic aluminium (Al) ions present in the rooting medium. Hypothetically OA complex Al in the root apoplast and/or rhizosphere and thus avoid its interaction with root cellular components and its entry in the root symplast. Two temporal patterns of root OA exudation are observed. In pattern I, OA release is rapidly activated after the contact of the root with Al ions while in pattern II there is a lag phase between the addition of Al and the beginning of OA release. Compounds other than OA have been detected in root exudates and are also correlated with Al resistance in plants. Plant species like buckwheat and tea show mechanisms of Al tolerance, which confer them...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Al resistance; Al toxicity; Anion channels; Root exudates; Transgenic plants.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100013

Plant tissue culture: Current status, opportunities and challenges
Provedor de dados: 118
Autores: García-Gonzáles,Rolando; Quiroz,Karla; Carrasco,Basilio; Caligari,Peter.
In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes. Current results have allowed plant biotechnology and its products -including transgenic plants with several traits- to be the most assimilated technology for farmers and companies, representing several benefits such as: 125 millions ha of transgenic crops in 2008, the reduction of pesticides application by up to 9% in the last ten years, transgenic plants with a better nutritional quality, mass propagation of selected and healthy plants, and the production of proteins for industrial or therapeutic use. The rapid and extensive...
Tipo: Journal article
Palavras-chave: In vitro; Micropropagation; Organogenesis; Plant bioreactors; Somatic embryogenesis; Transgenic plants.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300001