Consulta: subjectFacets:"Visceral leishmaniasis"
Registros recuperados: 39
Data/hora: 03/06/2024 06:39:31

Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs
Provedor de dados: 80
Autores: BLA,Verçosa; ACJ,Borges; FJM,Mendes; MLS,Costa; NB,Pereira; MN,Melo; IL,Mendonça; FAL,Costa; AC,Vasconcelos.
The skin has an important role in the transmission of visceral leishmaniasis (VL) as the infection pathway in dogs. To better characterize the inflammatory response of intact skin in VL, sixty infected dogs (30 symptomatic and 30 asymptomatic) and six non-infected controls were studied. Diagnosis of visceral leishmaniasis was confirmed by RIFI and ELISA; direct visualization of the parasite in bone marrow aspirate; imprints of popliteal lymph nodes, spleen, liver and skin; culture in NNN-phase liquid Schneider's medium; and PCR (performed only in the ear skin). Amastigote forms of the parasite in intact skin were found only in symptomatic dogs. Inflammatory infiltrates were observed in all groups, varying from intense and/or moderate in symptomatic to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Inflammation; Parasites; Leishmania chagasi.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300011

Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Provedor de dados: 80
Autores: Machado,JG; Hoffmann,JL; Krause,VLK; da Silva,AV; Dias-Melicio,LA; Langoni,H.
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunosuppression; Immunopathology; Mice; Interleukins.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013

Comparison of different diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis
Provedor de dados: 80
Autores: Sassaki,CY; Colodel,MM; Ferreira,I; Nogueira,FS; Lucheis,SB; Langoni,H; Rocha,NS.
Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Dogs; Visceral leishmaniasis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300015

Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
Provedor de dados: 80
Autores: Camargo,JB; Langoni,H; Troncarelli,MZ; Machado,JG; Lucheis,SB; Padovani,CR.
Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Dog; Diagnosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300007

Activity of the antiarrhythmic drug amiodarone against Leishmania (L.) infantum: an in vitro and in vivo approach
Provedor de dados: 80
Autores: Pinto,Erika G.; Tempone,Andre G.
Abstract  Background: Considering the high toxicity and limited therapies available for treating visceral leishmaniasis (VL), the drug repositioning approach represents a faster way to deliver new therapies to the market.  Methods: In this study, we described for the first time the activity of a potent antiarrhythmic, amiodarone (AMD), against L. (L.)infantum and its in vitro and in vivo activity.  Results: The evaluation against promastigotes has shown that amiodarone presents leishmanicidal effect against the extracellular form, with an IC50 value of 10 μM. The activity was even greater against amastigotes in comparison with promastigotes with an IC50 value of 0.5 μM. The selectivity index in relation to the intracellular form demonstrated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Visceral leishmaniasis; Amiodarone; In vitro; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100320

American visceral leishmaniasis: disease control strategies in dracen microregion in alta paulista, SP, Brazil
Provedor de dados: 80
Autores: D'Andrea,LAZ; Camargo-Neves,VLF; Sampaio,SMP; Kronka,SN; Sartor,IF.
Despite measures adopted to control American visceral leishmaniasis (AVL), the disease is spreading in a fast and worrying way throughout western São Paulo state. The aim of this work was to study the variables involved in the disease cycle as well as the effectiveness of controlling measures. The study was carried out in the microregion of Dracena, which is composed of twelve cities and belongs to Alta Paulista, a region of western São Paulo. The necessary data were provided by the Superintendence for Endemic Disease Control and Adolfo Lutz Institute, Regional Laboratory of Presidente Prudente. From August 2005 to January 2008, the following factors were observed: detection of phlebotomine sandflies in the cities and periods in which dogs or humans were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Seroprevalence; Control strategies; São Paulo; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200012

Impact of leishmaniasis on public health
Provedor de dados: 80
Autores: Camargo,L. B; Langoni,H.
Leishmaniasis is a parasitic zoonosis caused by protozoans of the genus Leishmania transmitted by insects known as phlebotomines, which are found in wild or urban environments. It affects domestic and wild animals and transmission to man happens by accident. The disease occurs in tropical and sub-tropical areas, mainly in Asia, Europe, Africa, and the Americas. There are two forms that affect man: American cutaneous leishmaniasis (ACL) and American visceral leishmaniasis (AVL). The latter is caused by three species of Leishmania: Leishmania (Leishmania) donovani, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) chagasi, which are grouped in the Leishmania (Leishmania) donovani complex. Wild reservoir hosts of L. chagasi known so far are foxes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; Visceral leishmaniasis; Leishmania.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400002

Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
Provedor de dados: 80
Autores: Paiva-Cavalcanti,M; Regis-da-Silva,CG; Gomes,YM.
In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Molecular diagnosis; Visceral leishmaniasis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400004

Electroantennographic responses of the Lutzomyia (Lutzomyia) longipalpis (Lutz &amp;amp; Neiva) (Diptera: Psychodidae) to 1-octen-3-ol
Provedor de dados: 82
Autores: SANT'ANA,ADSON L.; EIRAS,ALVARO E.; CAVALCANTE,REGINALDO R.
Octenol (1-octen-3-ol) is a kairomone used by haematophagous insects to locate their vertebrate hosts. However, effect of 1-octen-3-ol on Lutzomyia (Lutzomyia) longipalpis (Lutz &amp;amp; Neiva) has never been studied. The present work evaluated the electrophysiological (EAG) responses of female L. (Lutzomyia) longipalpis. Air current, air pulse and solvent (hexane) pulse were used as control stimuli. The logarithmic concentrations of 1-octen-3-ol 10 a 10(6) etag/50µl of solvent were tested. Significant olfactory responses were observed in the concentration of 1-octen-3-ol from 10³ etag/50ul with the greatest response at concentration of 10(6) etag/50ul (-3,33mV). Dose-dependency was observed, as the concentration increased, so did the electrophysiological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Chemoreception; Olfaction; Semiochemicals; Visceral leishmaniasis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000100002

Study on the bacterial midgut microbiota associated to different Brazilian populations of Lutzomyia longipalpis (Lutz &amp;amp; Neiva) (Diptera: Psychodidae)
Provedor de dados: 82
Autores: Gouveia,Cheryl; Asensi,Marise D.; Zahner,Viviane; Rangel,Elizabeth F.; Oliveira,Sandra M.P. de.
The bacterial community associated with the midgut of three Brazilian Lutzomyia longipalpis (Lutz &amp;amp; Neiva) populations, two from endemic areas for visceral leishmaniasis (Jacobina, Bahia State and São Luís, Maranhão State) and one from a non-endemic area (Lapinha Cave, Minas Gerais State), was identified. Five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. The species identification was based on molecular and traditional bacteriological methods. All bacteria were either affiliated to non-Enterobacteriaceae, such as Acinetobacter, Burkholderia, Flavimonas, Pseudomonas and Stenotrophomonas, or and to Enterobacteriaceae, such as Citrobacter, Enterobacter, Escherichia, Klebsiella,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sand fly; Visceral leishmaniasis; Microorganism; Gut microbiota.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000500016

Canine Visceral Leishmaniasis in a Doberman
Provedor de dados: 19
Autores: Sasani, F.; Jamshidi, Sh.; Mohebbali, M.
Palavras-chave: Dog; Doberman; Visceral leishmaniasis.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/323

Application of direct agglutination test and fast agglutination screening test for serodiagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
Provedor de dados: 19
Autores: Silva, E. S.; Schalig, H. D. F. H.; Kroon, N. C. M.; Schoone, G. J.; Gontijo, C. M. F.; Pacheco, Raquel S.; Brazil, R. P.
We have tested an indirect fluorescence technique (IFAT), fast agglutination screening test (FAST) and direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in serum and blood samples from patients with visceral and cutaneous leishmaniasis in Brazil. For visceral leishmaniasis the results obtained with DAT showed a sensitivity and specificity of 100% with a cut-off value of 1:800. The blood and serum samples could also be clearly read in FAST using a 1:100 dilution with the same high sensitivity. Both DAT and FAST were not able to detect significant amounts of antibodies in samples from cutaneous leishmaniasis patients.
Palavras-chave: Visceral leishmaniasis; Diagnosis; DAT; FAST.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/140

Genetic analysis of a recently detected urban population of Lutzomyia evansi (Diptera: Psychodidae)in Colombia
Provedor de dados: 99
Autores: Bejarano,Eduar Elías; Rojas,Winston; Uribe,Sandra; Vélez,Iván Darío; Porter,Charles H.
Lutzomyia evansi (Núñez-Tovar) is the vector of the parasite Leishmania infantum in rural zones of Northern Colombia. An attempt was made to determine the origin of a recently detected urban population of Lutzomyia evansi by genetically characterizing specimens from seven geographically distinct localities in the Colombian Caribbean. Insect specimens were collected in rural and urban environments of areas endemic for visceral leishmaniasis or free of the disease. Nine polymorphic sites, nine nucleotide haplotypes and a single aminoacid haplotype were found within the 315 bp fragment sequenced, corresponding to the 3' end of the cytochrome b mitochondrial gene. Paired genetic distances between the haplotypes, estimated with the Kimura two-parameters model,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sand flies; Genetics; Lutzomyia evansi; Visceral leishmaniasis; Colombia.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802009000100006

Histopathological and immunohistochemical study of the gastrointestinal tract from a dog naturally infected with Leishmania (Leishmania) chagasi: a case report
Provedor de dados: 45
Autores: Silva,F.L.; Tafuri,W.L.; Oliveira,M.R.; Tafuri,Wg. L.
Samples of stomach, duodenum, jejunun, ileum, cecum and colon were collected for Giemsa-smears ("imprints") from one asymptomatic mongrel dog, naturally infected with Leishmania (L) chagasi. Other fragments were obtained and fixed in formalin (10% and buffered) for histopathological and immunohistochemical studies. The immunohistochemistry was carried out by a streptavidin-peroxidase technique and it allowed to detect amastigote forms of Leishmania chagasi in the different paraffin gut sections. The principal lesion observed was a discrete to moderate chronic inflammatory reaction in the mucosa and submucosa in all fragments of the gastrointestinal tract (GIT). A chronic cellular exsudate was observed in all GIT tissues and it was composed by mononuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Visceral leishmaniasis; Histopathology; Immunohistochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400002

Clinical and histopathological features of myocarditis in dogs with visceral leishmaniasis
Provedor de dados: 45
Autores: Santos,F.P. dos; Pascon,J.P.E.; Pereira,D.T.P.; Anjos,B.L.; Mistieri,M.L.A.; Silveira,I.D.; Porciuncula,M.L.
Visceral leishmaniasis (VL) is a cosmopolitan parasitic zoonosis that can promote myocarditis and heart rate changes in canine and human hosts. Thus, histopathological aspects of the myocardium and clinical, hematological, biochemical, radiological and electrocardiographic data were evaluated in a group of 36 dogs naturally infected with VL (VLG) and compared to data from 15 non-infected dogs (CG=Control Group). A prevalence of asymptomatic dogs was present in the CG (100%) and polysymptomatic dogs in the VLG (66%). In addition, two dogs in the VLG demonstrated systolic murmurs in the mitral valve region: one with a II/VI intensity and the other with a III/VI intensity. The mean values of RBC, hemoglobin and hematocrit were lower in dogs in VLG and were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrocardiography; Leishmania sp.; Visceral leishmaniasis; Dogs.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601519

Epidemiological aspects of the first human autochthonous visceral leishmaniosis cases in Porto Alegre, Brazil
Provedor de dados: 55
Autores: Mahmud,Ibrahim Clós; Piassini,Letícia de Araujo Saraiva; Motta,Fabrizio; Behar,Paulo Renato Petersen; Souza,Getúlio Dornelles.
ABSTRACT Human visceral leishmaniasis is a growing anthropozoonosis in Brazil, and particularly in the southern region of the country. It is an infectious disease transmitted to humans, dogs and other animals in urban and rural areas of the Americas, mainly due to the bite of Lutzomya longipalpis infected with Leishmania infantum. This article aims to portray the current epidemiological situation of the human visceral leishmaniasis arrival in Porto Alegre city, located in the southern region of Brazil. It is a descriptive study, a case series and a critical review. Six human cases with human visceral leishmaniasis were notified by the date of conclusion of the study, all human visceral leishmaniasis cases were diagnosed at late stage, leading to four deaths.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Visceral leishmaniasis; Fever caused by sandflies; Communicable diseases; Leishmania infantum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200124

Epidemiological profile of leishmaniasis at a reference service in the state of Alagoas, Brazil, from january 2000 to september 2008
Provedor de dados: 55
Autores: Nunes,Willian da Silva; Araújo,Sócrates Rios; Calheiros,Cláudia Maria Lins.
Leishmaniasis is a parasitic disease found in the continents of Europe, Asia, Africa and the Americas. In Brazil, the disease is an important public health problem, occurring in most states, but mainly in the Northeast region of the country, with the state of Alagoas having a significant number of confirmed cases. The present study establishes and discusses the epidemiological profile of cases of leishmaniases treated at the Reference Center of Alagoas, Hospital Escola Hélvio de Farias Auto, between January 2000 and October 2008. During thisperiod, records of 2,104 patients notified to the hospital Epidemiological Surveillance Center were reviewed. The epidemiology of leishmaniasis, both visceral and tegumentary, has shown a profile that has changed over...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: American tegumentary leishmaniasis; Visceral leishmaniasis; Epidemiology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400005

Comparison of conventional methods for diagnosis of visceral leishmaniasis in children of the Center-West Region of Brazil
Provedor de dados: 55
Autores: Brustoloni,Yvone M.; Cunha,Rivaldo V.; Dorval,Maria Elizabeth; Oshiro,Elisa T.; Pontes,Elenir R.J.C.; Oliveira,Ana Lúcia L.; Hillebrand,Lilian; Ribeiro,Luis Felipe.
In Brazil, sophisticated techniques currently employed for diagnosis of visceral leishmaniasis, such as polymerase chain reaction-based assays, are only available in major research centers, whereas conventional methods are still used in many areas where the disease occurs. In the state of Mato Grosso do Sul, in the country's Center-West Region, visceral leishmaniasis has recently emerged in many cities, and duration of the disease, from the onset of symptoms to diagnosis, has been short. Considering that results of diagnostic tests may depend on the phase of the disease, we compared direct examination of bone marrow aspirates (BMAs), BMA culture, and serology by Indirect Immunofluorescence Antibody Test (IFAT) for diagnosis in children, according to time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Kala-azar; Diagnosis; Direct microscopy; Immunological tests.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100023

Urine as a promising sample for Leishmania DNA extraction in the diagnosis of visceral leishmaniasis - a review
Provedor de dados: 55
Autores: Bezerra,Gilberto Silva Nunes; Barbosa Júnior,Walter Lins; Silva,Elis Dionísio da; Leal,Nilma Cintra; Medeiros,Zulma Maria de.
ABSTRACT Visceral leishmaniasis is a serious and debilitating infection with high fatality rate in tropical and subtropical countries. As clinical symptoms of visceral leishmaniasis are not so specific, confirmatory diagnostic methods with high sensitivity and specificity are needed. Noninvasive methods have been developed using urine as a clinical sample for visceral leishmaniasis diagnosis. In fact, there is a clear correlation between kidney impairment and Leishmania DNA in urine. However, it has been proved that Leishmania nucleic acid may also be isolated from patients without any sign of renal involvement. Even though urine has become a promissing biological sample, it is still not widely used due to several issues, such as (i) incomprehension of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Renal involvement; Diagnosis; Urine sample; DNA extraction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200111

Pneumocystis carinii pneumonia, pulmonary tuberculosis and visceral leishmaniasis in an adult HIV negative patient
Provedor de dados: 55
Autores: Toledo Jr.,Antonio Carlos; Castro,Márcio Rodrigues de.
This is a case report of a 29 year old male with pneumocystis pneumonia and tuberculosis, and who was initially suspected of having HIV infection, based on risk factor analyses, but was subsequently shown to be HIV negative. The patient arrived at the hospital with fever, cough, weight loss, loss of appetite, pallor, and arthralgia. In addition, he was jaundiced and had cervical lymphadenopathy and mild heptosplenomegaly. He had interstitial infiltrates of the lung, sputum smears positive for Mycobacterium tuberculosis and Pneumocystis carinii, and stool tests were positive for Strongyloides stercoralis and Schistosoma mansoni. He was diagnosed as having AIDS, and was treated for tuberculosis, pneumocystosis, and strongyloidiasis with a good response. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pneumocystis carinii; Visceral leishmaniasis; Tuberculosis; HIV diagnosis; Opportunistic infections.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000300008

Post-kala-azar dermal leishmaniasis associated with AIDS
Provedor de dados: 55
Autores: Bittencourt,Achiléa; Silva,Nancy; Straatmann,Andréa; Nunes,Victor Luiz Correia; Follador,Ivonise; Badaró,Roberto.
Post-kala-azar dermal leishmaniasis (PKDL) is rarely reported in South America. In spite of the fact that there are many reports about the association of visceral leishmaniasis and AIDS, PKDL is very uncommon in HIV-positive patients, and so far only four cases have been documented in the literature. We present another case with unusual clinicopathological aspects. The patient, a 28-year-old male, from Salvador, Bahia (an endemic area) presented with clinical manifestations of visceral leishmaniasis three years after the diagnosis of AIDS. During treatment for visceral leishmaniasis he developed disseminated miliary papules. Microscopically, the skin biopsy showed a "saw-tooth" pattern with a lichenoid mononuclear infiltrate simulating lichen planus. The...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Visceral leishmaniasis; Post-kala-azar dermal leishmaniasis; AIDS.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000600008

Increased levels of IL-10, IL-12, and IFN- in patients with visceral leishmaniasis
Provedor de dados: 55
Autores: Khoshdel,A.; Alborzi,A.; Rosouli,M.; Taheri,E.; Kiany,S.; Javadian,M.H.
Visceral leishmaniasis remains a serious public health problem in developing countries. Cytokines have a crucial role in the pathogenesis of this disease. We evaluated plasma levels of IL-10, IL-12 and IFN- in 32 patients with active visceral lieshmaniasis, in 29 siblings of the patients and in 23 normal individuals in an endemic area to look for correlations between the clinical outcome of infection and the plasma cytokine levels. An analysis was made with a skin test and a quantitative sandwich enzyme immunoassay. Data were analyzed with the Mann Whitney test and the Kruskal Wallis test. The cytokine levels were significantly higher in the patients than in the control groups. We concluded that normalization of the plasma level of IFN- can serve as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Interleukin; Interferon.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100010

Are opossums a relevant factor associated with asymptomatic Leishmania infection in the outskirts of the largest Brazilian cities?
Provedor de dados: 55
Autores: Carranza-Tamayo,César Omar; Werneck,Guilherme Loureiro; Romero,Gustavo Adolfo Sierra.
Abstract A population survey was conducted to explore the prevalence and factors associated with Leishmania infection in the Fercal region of the Federal District. The Fercal region is a group of neighborhoods in Brasília in which the first cases of visceral leishmaniasis were described. Leishmania infection was established by a positive leishmanin test. Although other tests were performed in the study (an immunochromatographic assay (Kalazar detect®) and a molecular assay), only the leishmanin skin test provided sufficient results for the measurement of the disease prevalence. Data on the epidemiological, clinical and environmental characteristics of individuals were collected along with the diagnostic tests. After sampling and enrollment, seven hundred...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Brazil; Didelphis albiventris; Leishmanin.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200119

Congenitally transmitted visceral leishmaniasis: report of two brazilian human cases
Provedor de dados: 55
Autores: Mescouto-Borges,Myrlena Regina Machado; Maués,Érika; Costa,Dorcas Lamounier; Pranchevicius,Maria Cristina da Silva; Romero,Gustavo Adolfo Sierra.
Visceral leishmaniasis is a relevant public health problem worldwide. Most of the reported cases in Latin America are from Brazil. Herein we report two human cases of congenitally transmitted visceral leishmaniasis in two patients who developed symptoms during pregnancy. The diagnosis was made by visual examination of Leishmania parasites in bone marrow aspirates of the mothers and by detecting parasite kDNA in bone marrow samples of the newborn children using polymerase chain reaction.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Congenital transmission; Visceral leishmaniasis; Leishmania infantum; Pregnancy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200024

Clinical presentation and renal evaluation of human visceral leishmaniasis (kala-azar): a retrospective study of 57 patients in Brazil
Provedor de dados: 55
Autores: Daher,Elizabeth F.; Evangelista,Leandro F.; Silva Júnior,Geraldo B.; Lima,Rafael S.A.; Aragão,Eveline B.; Arruda,Germana A.J.C.; Galeano,Noemi M.F.; Mota,Rosa M.S.; Oliveira,Rodrigo A.; Silva,Sônia L.
Visceral leishmaniasis is an endemic disease caused by various species of Leishmania. We made a retrospective study of 57 consecutive patients with visceral leishmaniasis in Brazil. Patients with visceral leishmaniasis were identified using the registries of the São José Infectious Diseases Hospital. The sample was divided into two groups: patients with serum creatinine (Scr) &lt;1.3mg/dL and Scr &gt; 1.3mg/dL. We compared these two groups for differences in clinical manifestations and laboratory features. Patients' mean age was 28 ± 18 years old; 74% were male. The main clinical symptoms and signs presented in the initial evaluation were: fever (97%), splenomegaly (96.4%), weight loss (95.5%), pallor (93.6%), cough (89.7%), hepatomegaly (87.2%), asthenia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Kala-azar; Symptoms; Acute renal failure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400015

Monoclonal gammopathy associated with visceral leishmaniasis
Provedor de dados: 55
Autores: Sharma,Vishal; Agarwal,Mukul P; Giri,Subhash.
Monoclonal gammopathy can accompany diverse conditions and is usually benign. It should be distinguished from monoclonal gammopathy of undetermined significance (MGUS) which can rarely turn malignant. Visceral leishmaniasis has only rarely been associated with monoclonal gammopathy. We describe the case of a 55-year-old male who had monoclonal gammopathy associated with visceral leishmanisais, which reversed with stibogluconate therapy.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Visceral leishmaniasis; Monoclonal gammopathy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300018

Post-kala-azar dermal leishmaniasis associated with AIDS
Provedor de dados: 55
Autores: Bittencourt,Achiléa; Silva,Nancy; Straatmann,Andréa; Nunes,Victor Luiz Correia; Follador,Ivonise; Badaró,Roberto.
Post-kala-azar dermal leishmaniasis (PKDL) is rarely reported in South America. In spite of the fact that there are many reports about the association of visceral leishmaniasis and AIDS, PKDL is very uncommon in HIV-positive patients, and so far only four cases have been documented in the literature. We present another case with unusual clinicopathological aspects. The patient, a 28-year-old male, from Salvador, Bahia (an endemic area) presented with clinical manifestations of visceral leishmaniasis three years after the diagnosis of AIDS. During treatment for visceral leishmaniasis he developed disseminated miliary papules. Microscopically, the skin biopsy showed a "saw-tooth" pattern with a lichenoid mononuclear infiltrate simulating lichen planus. The...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Visceral leishmaniasis; Post-kala-azar dermal leishmaniasis; AIDS.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300009

Kidney involvement in leishmaniasis — a review
Provedor de dados: 55
Autores: Silva Junior,Geraldo Bezerra da; Barros,Elvino José Guardão; Daher,Elizabeth De Francesco.
Leishmaniasis is an infectious disease caused by protozoa of the genus Leishmania transmitted by insects of the genus Lutzomyia sp. or Phlebotomus sp. The main syndromes are cutaneous leishmaniasis, mucocutaneous leishmaniasis, visceral leishmaniasis (kala-azar) and post-kala-azar dermal leishmaniasis. This article reviews kidney involvement in cutaneous and visceral leishmaniasis, highlighting the aspects of their pathophysiology, clinical manifestations, histopathological findings, outcome and treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; American cutaneous leishmaniasis; Kala-azar; Kidney disease.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400434

Detection of specific IgE antibodies in parasite diseases
Provedor de dados: 56
Autores: Souza-Atta,M.L.B.; Araújo,M.I.; D'Oliveira Júnior,A.; Ribeiro-de-Jesus,A.; Almeida,R.P.; Atta,A.M.; Carvalho,E.M.
Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: IgE antibodies; Immunoassays; Schistosomiasis mansoni; Visceral leishmaniasis; Kala-azar.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008

Serum cytokine profile in the subclinical form of visceral leishmaniasis
Provedor de dados: 56
Autores: Gama,M.E.A.; Costa,J.M.L.; Pereira,J.C.R.; Gomes,C.M.C.; Corbett,C.E.P.
The factors determining the development or not of visceral leishmaniasis (VL) have not been completely identified, but a Leishmania-specific cellular immune response seems to play a fundamental role in the final control of infection. Few studies are available regarding the production of cytokines in the subclinical form of VL, with only the production of IFN-g and TNF-a known. The aim of the present study was to identify immunological markers for the oligosymptomatic or subclinical form of VL. A prospective cohort study was conducted on 784 children aged 0 to 5 years from an endemic area in the State of Maranhão, Brazil, between January 1998 and December 2001. During 30 consecutive months of follow-up, 33 children developed the oligosymptomatic form of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Cytokine levels; Immunological aspects; Subclinical form; Oligosymptomatic form.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100018

CD4+ T cells participate in the nephropathy of canine visceral leishmaniasis
Provedor de dados: 56
Autores: Costa,F.A.L.; Guerra,J.L.; Silva,S.M.M.S.; Klein,R.P.; Mendonça,I.L.; Goto,H.
Renal involvement in visceral leishmaniasis (VL) is very frequent. The renal lesions of humans and dogs are similar but their pathogenesis has not been clearly elucidated. There is growing evidence that the cellular immune response is involved in the pathogenesis of immunologically mediated glomerulonephritis. Since T cells could participate in the pathogenesis of nephropathy, in the present study we investigated the possible involvement of CD4+ and CD8+ T cells in the nephropathy of canine VL. Six dogs naturally infected with Leishmania (Leishmania) chagasi from the endemic area in the Northeast of Brazil, the town of Teresina in the State of Piauí, were studied. An expressive inflammatory infiltrate of CD4+ T cells both in glomeruli and in interstitium...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visceral leishmaniasis; Dog; Leishmania (Leishmania) chagasi; Nephropathy; Immunoglobulin; T cells.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200009

Anti-leishmania antibodies in cerebrospinal fluid from dogs with visceral leishmaniasis
Provedor de dados: 56
Autores: Lima,V.M.F.; Gonçalves,M.E.; Ikeda,F.A.; Luvizotto,M.C.R.; Feitosa,M.M.
Visceral leishmaniasis in Brazil is caused by Leishmania (Leishmania) chagasi and the dog is its most important reservoir. The clinical features in dogs include loss of weight, lymphadenopathy, renal failure, skin lesions, fever, hypergammaglobulinemia, hepatosplenomegaly, anemia, and, rarely, neurological symptoms. Most infected animals develop active disease, characterized by high anti-leishmania antibody titers and depressed lymphoproliferative ability. Antibody production is not primarily important for protection but might be involved in the pathogenesis of tissue lesions. An ELISA test was used to determine if there is an association between neurological symptoms and the presence of anti-L. chagasi antibodies in cerebrospinal fluid (CSF). Thirty serum...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Cerebrospinal fluid; Dogs; Antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400010

Immunity and immunosuppression in experimental visceral leishmaniasis
Provedor de dados: 56
Autores: Goto,H.; Lindoso,J.A.L.
Leishmaniasis is a disease caused by protozoa of the genus Leishmania, and visceral leishmaniasis is a form in which the inner organs are affected. Since knowledge about immunity in experimental visceral leishmaniasis is poor, we present here a review on immunity and immunosuppression in experimental visceral leishmaniasis in mouse and hamster models. We show the complexity of the mechanisms involved and differences when compared with the cutaneous form of leishmaniasis. Resistance in visceral leishmaniasis involves both CD4+ and CD8+ T cells, and interleukin (IL)-2, interferon (IFN)- gamma, and IL-12, the latter in a mechanism independent of IFN- gamma and linked to transforming growth factor (TGF)-ß production. Susceptibility involves IL-10 but not IL-4,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunosuppression; T cell; Cytokines; Mice; Hamster.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400020

Detection of immunoglobulin G in the lung and liver of hamsters with visceral leishmaniasis
Provedor de dados: 56
Autores: Mathias,R.; Costa,F.A.L.; Goto,H.
Several organs are affected in visceral leishmaniasis, not only those rich in mononuclear phagocytes. Hypergammaglobulinemia occurs during visceral leishmaniasis; anti-Leishmania antibodies are not primarily important for protection but might be involved in the pathogenesis of tissue lesions. The glomerulonephritis occurring in visceral leishmaniasis has been attributed to immune complex deposition but in other organs the mechanism has not been studied. In the current study we demonstrated the presence of IgG in the lung and liver of hamsters with visceral leishmaniasis. Hamsters were injected intraperitoneally with 2 x 10(7) amastigotes of Leishmania (Leishmania) chagasi and the presence of IgG in the liver and lung was evaluated at 7, 15, 30, 45, 80 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Immunopathology; Immunoglobulin G; Liver; Lung.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400015

Leishmania (Leishmania) chagasi-infected mice as a model for the study of glomerular lesions in visceral leishmaniasis
Provedor de dados: 56
Autores: Prianti,M.G.; Yokoo,M.; Saldanha,L.C.B.; Costa,F.A.L.; Goto,H.
Renal involvement in visceral leishmaniasis (VL) is very frequent but the pathogenesis of this nephropathy is poorly understood. In previous studies using dogs with VL we have detected new immunopathological elements in the glomeruli such as T cells and adhesion molecules. Although Leishmania (Leishmania) chagasi-infected dogs and hamsters are considered to be good models for VL, their use is limited for immunopathologic studies. The use of isogenic mouse strains susceptible to L. (L.) chagasi infection was an alternative but, on the other hand, the renal lesions of these animals have not yet been characterized. Thus, our purpose in the present study was to characterize mice infected with L. (L.) chagasi as a suitable model to study VL nephropathy. Kidney...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visceral leishmaniasis; Glomerulonephritis; Mice; Model; Leishmania (Leishmania) chagasi.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600011

Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli
Provedor de dados: 58
Autores: Vaz,Michelle Rossana Ferreira; França,Ricardo Luiz Soares de; Andrade,Sirtys Santos Lessa de; Sousa Junior,Francisco Canindé de; Santos,Everaldo Silvino dos; Martins,Daniella Regina Arantes; Macedo,Gorete Ribeiro de.
With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivation; Recombinant protein; Visceral leishmaniasis; Escherichia coli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021

Parasite load, iNOS and cytokine profiles, and histopathological aspects of Leishmania infantum infection in dogs with different clinical presentations
Provedor de dados: 65
Autores: Vasconcelos,Tassia Cristina Bello de; Bruno,Sávio Freire; Miranda,Luisa Helena Monteiro de; Conceição-Silva,Fátima; Belo,Vinícius Silva; Figueiredo,Fabiano Borges.
ABSTRACT:  Visceral leishmaniasis (VL) is a zoonotic disease with a canine urban reservoir in South America. Dogs from an endemic area within Brazil, which were naturally infected with Leishmania infantum, and those presenting severe clinical (SC), mild, or no clinical (MNC) disease, were evaluated. Parasite load, histopathology, and cytokine and iNOS mRNA expressions were assessed in the spleen and liver in order to determine the potential markers for disease susceptibility or resistance. As a result, dogs with both SC and MNC had high parasite loads; IFN-γ was the most expressive cytokine in both organs, along with IL-6 and IL-4 being detected in the spleen and liver, and IL-10 only in liver. The hepatic tissue presented higher medians for IFN-γ and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunopathology; Canine infection; Histopathology; Canine cytokines.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001000501

High frequency of visceral leishmaniasis in dogs under veterinary clinical care in an intense transmission area in the state of Tocantins, Brazil
Provedor de dados: 65
Autores: Santos,Helcileia Dias; Galvão,Samara Rocha; Dias,Francisca Elda Ferreira; Ribeiro,Taiã Mairon Peixoto; Negreiros Filho,Osmar; Sousa,Sebastiana Adriana Pereira; Minharro,Silvia.
ABSTRACT: A direct search for parasites were used as the diagnostic test to determine the frequency of  Leishmania spp. infection in dogs ( Canis lupus familiaris ) under veterinary clinical care in the city of Araguaína, Tocantins, Brazil. For this approach, lymph node cell samples were collected using needle aspiration from 649 dogs of different breeds and ages. Two hundred and sixty four (40.7%) dogs tested positive for amastigote forms of  Leishmania spp. Furthermore, 202 (76.5%) dogs that tested positive showed some clinical sign of disease, while 62 (28.4%) dogs were asymptomatic. Dogs &lt;2 years old or those that lived alongside poultry species in peri-domicile areas had a greater chance of infection (P&lt;0.05). Our results revealed the importance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Cytodiagnosis; Risk factors; Prevention and control.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300501

Leishmania chagasi in dogs from the city of Jaciara, Mato Grosso, Brazil
Provedor de dados: 65
Autores: Lazari,Patrícia; Almeida,Arleana do Bom Parto Ferreira de; Dutra,Valéria; Nakazato,Luciano; Dias,Álvaro Felipe de Lima Ruy; Brito,Veruska Nogueira de; Oliveira,Cenita Maria; Sousa,Valéria Régia Franco.
ABSTRACT: The aim of this study was to identify the Leishmania species in Jaciara dogs; visceral leishmaniasis (VL) is endemic in the county of Jaciara. A comparison among the PCR results for the spleen, bone marrow, skin and blood of 101 dogs with VL-reactive serum were evaluated. Spleen tissue showed the highest detection percentage, followed by bone marrow. In 97 (96.04%) dogs, the Leishmania DNA detected was confirmed as L. chagasi, emphasizing the importance of aetiological identifications in sympatric areas with other trypanosomatids.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Visceral leishmaniasis; PCR; Different tissues.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200315