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Non-linear multivariable predictive control of an alcoholic fermentation process using functional link networks
Provedor de dados: 52
Autores: Meleiro,Luiz Augusto da Cruz; Costa,Aline Carvalho da; Maciel Filho,Rubens.
In this work a MIMO non-linear predictive controller was developed for an extractive alcoholic fermentation process. The internal model of the controller was represented by two MISO Functional Link Networks (FLNs), identified using simulated data generated from a deterministic mathematical model whose kinetic parameters were determined experimentally. The FLN structure presents as advantages fast training and guaranteed convergence, since the estimation of the weights is a linear optimization problem. Besides, the elimination of non-significant weights generates parsimonious models, which allows for fast execution in an MPC-based algorithm. The proposed algorithm showed good potential in identification and control of non-linear processes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extractive alcoholic fermentation; Functional link neural networks; Non-linear predictive control.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400002

Production of Beauveria bassiana Air Conidia by Means of Optimization of Biphasic System Technology
Provedor de dados: 52
Autores: Gouli,Vladimir; Gouli,Svetlana; Kim,Jae Su.
Air conidia production of Beauveria bassiana, strain CA-603 was studied based on modified diphasic system. The biomass yield obtained in the first phase based on submerged cultivation of fungus was processed using methodology providing different contact with air space. Our study indicated that productivity of the second stage of diphasic system is had inversely proportional dependence on depth of liquid fungal biomass. Increase of biomass depth is significantly decreased production of air conidia. Two methodology of biomass processing extending contact biomass with air space including distribution of fungal material on surface of hygroscopic paper and starch packaging peanuts were investigated. The novel substrates provided optimal contact between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beauveria bassiana; Aerial conidia; Biphasic production technology; Starch peanuts; Roll-method.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400571

Technical Losses Reduction in Underground Reticulated Distribution Systems using Artificial Neural Networks and Smart Grid Features
Provedor de dados: 52
Autores: Cambraia,Mario Sergio; Brandão Júnior,Augusto Ferreira; Rosa,Luiz Henrique Leite.
ABSTRACT This work presents the methodology, development and testing of an autonomous system, based on Artificial Neural Networks (ANN), for the reduction of technical losses in reticulated underground systems through the optimal control of the capacitor banks (CBs) present in the grid. The proposed methodology includes Smart Grid features, including practical solutions for current transformers positioning in underground networks, collecting field measurements for the Distribution Operation Centre (DOC) and real-time control of field equipment (capacitors banks). The steps of the proposed methodology and the main aspects of the development of the system are also described, as well as the tests performed to prove the results and validate the system.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smart grids; Artificial neural networks; Underground reticulated networks; Technical losses.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200205

Influence of Cooling on the Glycolysis Rate and Development of PSE (Pale, Soft, Exudative) Meat
Provedor de dados: 52
Autores: Pedrão,Mayka Reghiany; Kato,Talita; Soares,Adriana Lourenço; Ida,Elza Iouko; Coró,Fábio Augusto Garcia; Grespan,Moises; Paião,Fernanda; Shimokomaki,Massami.
The aim of this work was to evaluate pH values fall rate in chicken breast meat under commercial refrigeration processing conditions and the development of PSE (pale, soft, exudative) meat. Broiler breast samples from the Cobb breed, both genders, at 47 days of age (n = 100) were taken from refrigerated carcasses (RS) immersed in water and ice in a tank chilled at 0°C (±2). pH and temperature (T) values were recorded at several periods throughout refrigeration in comparison to samples left at room T as control (CS). The ultimate pH (pHu) value of 5.86 for RS carcasses were only reached at 11°C after 8.35 h post mortem (PM) while, for CS samples, pHu value was 5.94 at 22°C after 4.08 h PM. Thus, under commercial refrigeration conditions, the glycolysis rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Post mortem management; Commercial processing refrigeration; Water chiller.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200272

Cultivation of Agaricus blazei on Pleurotus spp. spent substrate
Provedor de dados: 52
Autores: Gern,Regina Maria Miranda; Libardi Junior,Nelson; Patrício,Gabriela Nunes; Wisbeck,Elisabeth; Chaves,Mariane Bonatti; Furlan,Sandra Aparecida.
The aim of this work was the use of Pleurotus ostreatus and Pleurotus sajor-caju for the previous lignocellulolytic decomposition of banana tree leaf straw and the further use of the degraded straw as substrate for the culture of Agaricus blazei. For optimising the production of A. blazei in terms of yield (Y%) and biological efficiency (BE%), adjustments to the composition of the substrate were evaluated in a 2(5) experimental design. The following components were tested in relation to % of substrate dry mass: urea (1 and 10%), rice bran (10 or 20%) or ammonium sulphate (0 or 10%), inoculum (10 or 20%) and the casing material (subsoil or burned rice husks). The best results (79.71 Y% and 6.73 BE%) were found when the substrate containing 10% of rice bran,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agro-industrial Wastes; Basidiomycetes; Edible Mushrooms; Fungi; Lignocellulosic Degradation; Solid State Fermentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400024

Quality evaluation and sugar beet postharvest conservation under modified atmosphere
Provedor de dados: 52
Autores: Scalon,Silvana de Paula Quintão; Scalon Filho,Homero; Sandre,Tatiane Almeida; Silva,Eulene Francisco da; Krewer,Eliane Caetano Domingos.
This work had as objective the evaluation of sugar beets postharvest conservation under modified atmosphere and at environmental temperature (15 - 26° C). The experiment was entirely guided at a randomized design with three factorial sizes (small,, medium and large) and two packings (PVC and without packing) in three repetitions of five beets. They were appraised in the beginning and at the end of storage, for pH;Total Soluble Solids (TSS) and TotalTitration Acidity (TTA) contents. During the storage, the percentage of weight loss percentage was also appraised. It was observed that the packing reduced between in eight to ninefold the weight loss, and the small beets presented smaller weight loss than the large ones. After 14 days they were stored, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta vulgaris; Postharvest; Modified atmosphere.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200007

Spirulina platensis effects on the levels of total cholesterol, HDL and triacylglycerols in rabbits fed with a hypercholesterolemic diet
Provedor de dados: 52
Autores: Colla,Luciane Maria; Muccillo-Baisch,Ana Luiza; Costa,Jorge Alberto Vieira.
In this work, hypercholesterolemia was induced in rabbits by feeding them a high cholesterol diet (CD, 350 mg/d) and the effects of supplementing this diet with 0.5 g/d Spirulina platensis was evaluated by measuring the levels of serum total-cholesterol (TC), triacylglycerols (TAG) and high-density lipoprotein (HDL-cholesterol) at the start of the experiment and after 30 d and 60 d. It was found that the levels of serum cholesterol decreased from 1,054±101 mg.dL-1 in the rabbits fed a CD without S. platensis to 516±163 mg.dL-1 to those fed with a high cholesterol diet supplemented with S. platensis (significant at p &lt;0.0001). The addition of Spirulina to the cholesterolemic diet did not cause significative decrease on the levels of triacylglycerols in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spirulina platensis; Cholesterol; Rabbits.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200022

Digenea in the surubim Pseudoplatystoma corruscans (Spix and Agassiz, 1829) (Siluriformes: Pimelodidae) of the upper São Francisco River, State of Minas Gerais, Brazil
Provedor de dados: 52
Autores: Corrêa,Renata de Fátima da Silva Stravalli; Brasil-Sato,Marilia de Carvalho.
The specimens of surubim, Pseudoplatystoma corruscans (Spix and Agassiz, 1829), an important food fish with high commercial value in the Upper São Francisco River, were collected downstream from the Três Marias Dam, in the municipality of Três Marias, State of Minas Gerais, Brazil. Thirty-six individuals were examined and among the parasites, four species of Digenea were found: Witenbergia witenbergi Vaz, 1932 and Tylodelphys sp. (metacercarie), both with prevalence above 10%, followed by Acanthostomum gnerii (Szidat, 1958) and Acanthostomum sp.. The total length of the hosts did not influence the parasite prevalence. Witenbergia witenbergi occurred only in female hosts and the abundance of Tylodelphys sp. was higher in the males. These Digenea were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acanthostomum spp.; Digenea; Pseudoplatystoma corruscans; São Francisco River Tylodelphys sp.; Witenbergia witenbergi.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600013

Concentration determination of extracellular and intracellular red pigments produced by Monascus sp
Provedor de dados: 52
Autores: Hamano,Patricia Sanae; Orozco,Sandra Fernanda Bilbao; Kilikian,Beatriz Vahan.
In this work red pigments production was evaluated (by spectrophotometry) in semi-synthetic medium by rotary shaker cultures, utilizing seven strains of Monascus sp. The strains isolated at LEB/DEQ/EPUSP had a absorbance average of extracellular red pigments higher than the others strains (13.0 and 9.6 U, respectively) and the specific production of extracellular red pigments was from 1.7 until 3.5 times higher than the specific production of intracellular ones.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filamentous fungi; Monascus; Natural colorants; Red pigments; Cell disruption.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400006

Biodeterioration studies of thermoplastics in nature using indigenous bacterial consortium
Provedor de dados: 52
Autores: Anwar,Mohd. Shahbaz; Negi,Harshita; Zaidi,Mohd. Ghulam Haider; Gupta,Sanjay; Goel,Reeta.
Thermoplastics, poly vinyl chloride and low-density polyethylene were treated in the presence of indigenously developed bacterial consortium in laboratory and natural conditions. The consortium was developed using four bacteria, selected on the basis of utilization of PVC as primary carbon source, namely P. otitidis, B. aerius, B. cereus and A. pedis isolated from the plastic waste disposal sites in Northern India. The comparative in-vitro treatment studies as revealed by the spectral and thermal data, illustrated the relatively better biodegradation potential of developed consortium for PVC than the LDPE. Further, the progressive treatments of both the thermoplastics were conducted for three months under natural conditions. For this purpose,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodeterioration; Bioformulation; Thermoplastic; FT-IR; SEM; TG-DTG-DTA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300016

Chemical basis for beef charqui meat texture
Provedor de dados: 52
Autores: Youssef,Elza Y.; Garcia,Carlos Eduardo Rocha; Yamashita,Fábio; Shimokomaki,Massami.
This work evaluated the relationship of charqui meat (CHM) chemical composition with the tenderness throughout its production. CHM was prepared from beef Vastus lateralis of 4-5 years old. Shear force of fresh CHM showed an approx. 3-fold increase in toughness compared to the raw material while, in the case of cooked CHM it was 6-fold increased in relation to the raw charqui. The moisture content decreased by 39.0 and 58.0% (p&lt;0.05) for uncooked and cooked CHM, respectively, in relation to the raw material. Mathematical modeling of the influence of these meat components showed that shear force increased exponentially with the loss of moisture. The texture of CHM was the result of a multitude of factors involving myofibril proteins which promoted dynamic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen crosslinking; Water activity; Intermediate moisture meat product; Myofibril proteins.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400018

Fresh pasta production enriched with Spirulina platensis biomass
Provedor de dados: 52
Autores: Lemes,Ailton Cesar; Takeuchi,Katiuchia Pereira; Carvalho,João Carlos Monteiro de; Danesi,Eliane Dalva Godoy.
The aim of this work was to study the enrichment of Spirulina platensis in wheat flour to prepare fresh pasta to evaluate the green color and nutritional enrichment in addition to functional properties due to the presence of the bioactive compounds in the cyanobacterium. The pastas were evaluated for the centesimal composition, microbiological contamination, sensorial acceptance and technological characteristics such as cooking time, water absorption, volume displacement and loss of solids. The superior protein contents and the satisfactory technological and sensorial attributes compared with the control with no cyanobacterium showed the usefulness of incorporating S. platensis biomass in the fresh pastas. The microbiological quality was in compliance with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioactive compounds; Sensorial acceptance; Technological parameters.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500014

Effect of microalga Spirulina platensis (Arthrospira platensis) on hippocampus lipoperoxidation and lipid profile in rats with induced hypercholesterolemia
Provedor de dados: 52
Autores: Bertolin,Telma Elita; Pilatti,Dayane; Giacomini,Ana Cristina Vendrametto Varrone; Bavaresco,Caren Serra; Colla,Luciane Maria; Costa,Jorge Alberto Vieira.
Studies have been conducted on microalga Spirulina platensis (Arthrospira platensis) due to its therapeutic potential in several areas, including the capacity for preventing and decreasing the damages caused by hyperlipidemia and the antioxidant activity. The aim of the study was to evaluate the effect of microalga Spirulina platensis on hippocampus lipoperoxidation and lipid profile in rats with induced hypercholesterolemia during 60 days. The measurement of hippocampus lipoperoxidation did not demonstrate significant difference (p&gt;0.05) when Spirulina platensis was added to hypercholesterolemic diet. The evaluation of lipid profile showed that the administration of the microalga in therapeutic and preventive ways led to a significant protective effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spirulina; Arthrorspira; Lipids; Cholesterol; Antioxidant.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500024

Photo-fenton remediation of wastewaters containing agrochemicals
Provedor de dados: 52
Autores: Teixeira,Antonio Carlos Silva Costa; Mendes,Lucas; Stollar,Giselle; Guardani,Roberto; Nascimento,Cláudio Augusto Oller do.
The photochemical degradation of agrochemicals in aqueous solution by means of advanced oxidation processes (AOPs) was studied. The photo-Fenton process was evaluated in terms of the time evolution of dissolved organic carbon (COD) and chemical oxygen demand (DOC), their total removals, and increase in biodegradability of treated wastewater. Under the experimental conditions studied, the process showed to be superior to other AOPs, at any Fe(II) and H2O2 concentrations. The results pointed towards the use of solar irradiation and low cost commercial application.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Advanced Oxidation Processes; Photo-Fenton; Agrochemicals; Wastewater.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400026

Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
Provedor de dados: 52
Autores: Markovic,Marija; Grbic,Mihailo; Djukic,Matilda.
The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pineleaf pink; In vitro culture; BAP; NAA; Multiplication.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100403

Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors
Provedor de dados: 52
Autores: Sella,Sandra Regina Barroso Ruiz; Guizelini,Belquis Palácio; Vandenberghe,Luciana Porto de Souza; Medeiros,Adriane Bianchi Pedroni; Soccol,Carlos Ricardo.
Studies were conducted to evaluate Bacillus atrophaeus spores' production by solid-state fermentation (SSF) using sugarcane bagasse as support and soybean molasses as substrate at lab-scale in column bioreactors (forced aeration), plastic bags and Erlenmeyer flasks (aeration by diffusion). Different moisture contents (84%, 86% and 88%; 89%, 91% and 93%) and aeration rates (30mL/min, 45mL/min, 60mL/min and 90mL/min) were studied. The best condition for spore production (3.3x10(10) CFU.g-¹dry matter) in column bioreactor was 80% of initial humidity and no aeration. In Erlenmeyer flasks and plastic bags the best sporulation production reached 1.7 up to 4.7x10(10) CFU.g-1dry matter with 88-93% of initial moisture. The aeration rate had no significant effect on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus atrophaeus; Spores; Solid state fermentation; Column bioreactor; Biofilm.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700021

The epiphytic Cactaceae Hylocereus setaceus (Salm-Dick ex DC.) ralf bauer seed germination is controlled by light and temperature
Provedor de dados: 52
Autores: Simão,Edson; Socolowski,Fábio; Takaki,Massanori.
The effect of light and temperature on seed germination in Hylocereus setaceus was analyzed by isothermic incubations under continuous white light and darkness. The minimum temperature for germination was between 5 to 10°C and the maximum between 45 to 50°C. The optimum temperaturewais between 25 and 30°C. The involvement of phytochrome, by very low fluence response, in controlled seed germination was attained by incubation in a gradient of photoequilibrium of phytochrome. The results indicated that seeds of H. setaceus germinated in a wide range of temperature, under canopy and in open areas, but in complete darkness they did not germinate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endangered cactus; Phytochrome; Seed germination.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400011

Reaction of wheat genotypes to soil aluminum differential saturations
Provedor de dados: 52
Autores: Costa,Antonio; Campos,Luís Alberto Cogrossi; Riede,Carlos Roberto.
Ten wheat (Triticum aestivum) genotypes were evaluated in microplots with aluminum saturation of 0, 15, 30 and 45%, during 1994, in Londrina, Paraná, Brazil. The soil was a Distrofic Red Latosol (Typic Haplorthox), with 65% saturation of aluminum, amended with dolomitic lime. Variables evaluated included grain yield and yield components: ears.m-2, grains.ear-1 and the weight of 1000 grains. Genotypes differed in yield and yield components. Increasing aluminum saturation decreased yield, ears.m-2 and grains.ear-1, but did not alter thousand kernel weight. The genotypes reacted differently in relation to the toxic soil aluminum. Anahuac and IAPAR 29 were aluminum sensitive; OCEPAR 16, Trigo BR 18, and Trigo BR 23 were moderately sensitive; IAPAR 6, IAPAR 53,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Aluminum tolerance; Aluminum soil saturation; Yield components.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100004

Fisheries and conflicts in Guanabara Bay, Rio de Janeiro, Brazil
Provedor de dados: 52
Autores: Jablonski,Silvio; Azevedo,Alexandre de Freitas; Moreira,Luiz Henrique Arantes.
Despite the magnitude of domestic and industrial pollution, fishery yield registered in several landing points at Guanabara Bay, between April 2001 and March 2002 was slightly over 19,000 tons, corresponding to a value of US$ 4.8 million. When considered only the fish directed to the food market, the total catch amounted to about 6,300 tons and a value of US$ 3.7 million. Only a few fish species reached expressive densities compatible with commercial fisheries. Among small pelagic fish, Atlantic anchoveta and Brazilian sardinella were the dominant species, while in terms of demersal fish, croakers, mullets and catfishes comprised the main part of the catch. The absence of landing data previous to the spilling of 1.3 million liters of oil in January 2000...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fisheries; Fisheries statistics; Guanabara Bay; Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100010

Soybean β-Glucosidase immobilisated on chitosan beads and its application in soy drink increase the aglycones
Provedor de dados: 52
Autores: Grade,Luciana Carvalho; Moreira,Amanda Aleixo; Varea,Geni da Silva; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko; Ribeiro,Mara Lúcia Luiz.
The objective of this study was to investigate the immobilisation efficiency of soybean β-glucosidase (181.6 U/mL; 23.8 mg protein/mL) on activated chitosan beads. Central Composite Rotational Design (CCDR) 23 was used and the application of immobilised enzyme in commercial soy drink was evaluated. The activation of chitosan beads was achieved with established 2.5% glutaraldehyde, pH 7.5, 8 h incubation time (6 h with agitation and 2 h without agitation) at 37ºC. The highest immobilisation efficiency (%) of soybean β-glucosidase on chitosan beads obtained was 37.74 U/mL and 18.84 mg protein/4 chitosan beads at pH 7.5 and 20 h coupling time of enzyme-matrix (7 h with agitation and 13 h without agitation) at 4ºC. The immobilised enzyme incubated at 50ºC, pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-glucosidase soybean; Immobilisation; Chitosan; Aglycones.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500766

Embryonic stem-like cells derived from in vitro produced bovine blastocysts
Provedor de dados: 52
Autores: Freitas,Erika Regina Leal de; Sanches,Bruno Valente; Gambarini,Maria Lúcia; Oliveira Filho,Benedito Dias; Guillo,Lidia Andreu.
The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine blastocysts; ICM isolation; Embryonic stem cells; Gene markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009

Produção de Lactobacillus plantarum em Melaço de Cana-de-açúcar
Provedor de dados: 52
Autores: Villavicencio,Angel R. N.; Sant’Anna,Ernani S.; Tôrres,Regina C. O.
Comparative growth studies of Pediococcus pentosaceus were done in MRS (control) culture medium as well as in culture medium of 5% sugar cane blackstrap molasses (Broth 1) enriched with yeast extract (Broth 2) and enriched with beef extract (Broth 30. The experiment was carried out in a shaker (96 rpm) in 250 mL Erlenmeyers flasks with working volume of 150 mL, initial inoculum of about 10³ - 10(4) FCU/mL, at 35 ± 1ºC temperature and 28-hour fermentation. The biomass pruductivity was higher in MRS broth (0.0375 g/L.h), followed by Broth 2 (0.0275), Broth 3 (0.0189) and Broth 1 (0.0151). The higher viable cells number occured in MRS (13.96 log10 FCU/mL), followed by Broth 2 (12.09), Broth 3 (10.12) and Broth 1 (8.42).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediococcus pentosaceus; Starter culture; Molasses.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200015

Role of Natural Antioxidants in the Modulation of Plasma Amino Acid Pattern in Rats Exposed to Hemic Hypoxia
Provedor de dados: 52
Autores: Al-Rasheed,Nouf Mohamed; Fadda,Laila Mohamed; Al-Rasheed,Nawal Mohamed; Attia,Hala; Ali,Hanaa Mahmoud; El-Agami,Hanaa.
ABSTRACTThe aim of this work was to investigate whether the free radical scavengers, L-arginine (L-arg) and/or carnosine, either alone, or in combination would modulate tissue injury induced by hypoxia by measuring Fischer's ratio [concentrations of branched chain amino acids (BCAAs)/aromatic amino acids]. Decreased Fischer's ratios and increased malondialdehyde (MDA) led to pathogeneses of many diseases. Rats were injected with sodium nitrite (60 mg/kg) to establish hypoxia. They were treated with L-arg, (200 mg/ kg) and/or carnosine (200 mg/ kg) and their combination 24 and 1 h prior to sodium nitrite intoxication. The results revealed that hypoxia significantly decreased hemoglobin, arginine, citrulline and proline and increased sLDH, MDA , ammonia ,...
Palavras-chave: Natural antioxidants; Amino acids; Carnosine; L-arginine; Fisher's ratio; Urea.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500741

Gills of juvenile fish piaractus mesopotamicus as histological biomarkers for experimental sub-lethal contamination with the Organophosphorus Azodrin®400
Provedor de dados: 52
Autores: Rudnicki,Cácia Aparecida Mendes; Melo,Gisele Cristiane; Donatti,Lucélia; Kawall,Helena Gonçalves; Fanta,Edith.
Piaractus mesopotamicus is a freshwater native fish from rivers of the Paraná-Paraguay Basin and of the Pantanal region and has been used for repopulation programs in Brazil. Juvenile fishes were exposed to the sub-lethal dose of 1.08mg/L of the OP Azodrin®400 containing 0.43µL/L of the active principle monocrotophos for 96 h. A frequent pathology in the gills at all times of exposure was epithelial detachment, from minimal until 24 h of exposure, to intense after 48 h of contamination. Deformed pillar cells in the respiratory lamellae leading to irregular blood spaces and blood congestion, as well as hyperplasia and lamellar fusion were observed. These histopathologies suggested that 48 h after T0 was an important time when a reduction in the capability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater Fish; Organophosphorus; Monocrotophos; Morphology; Branchiae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600015

Composition and significance of glycosaminoglycans in the uterus and placenta of mammals
Provedor de dados: 52
Autores: Oliveira,Gleidson Benevides de; Vale,André Menezes do; Santos,Amílton Cesar dos; Moura,Carlos Eduardo Bezerra de; Rocha,Hugo Alexandre de Oliveira; Oliveira,Moacir Franco de.
Glycosaminoglycans (GAGs) are heteropolysaccharides in mammalian tissue and consist of repeated disaccharide units with mono-sulfated or non-sulfated monosaccharides. GAGs are important components of the Extracellular Matrix (ECM) with several physiological roles, in the recognition, migration, adhesion, proliferation and differentiation processes. They are also important in angiogenesis, blood homeostasis, immune reactions, follicule development and also in the development of pathologies such as infertility, tumors and metastases. It has been shown that the profile of glycosaminoglycans in the uterine and placental tissues is highly variable throughout the reproductive cycle and during pregnancy. It may be directly related to their physiological or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Hormonal fluctuation; Reproductive events; Euterium mammals; Pathology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400512

Combination vitamin C and vitamin E prevents enteric diabetic neuropathy in the small intestine in rats
Provedor de dados: 52
Autores: Zanoni,Jacqueline Nelisis; Hermes-Uliana,Catchia.
The present study evaluated the effects of supplementation with a combination of vitamin C and vitamin E on NADH-diaphorase-positive (NADH-d+) and neuronal nitric oxide synthase (nNOS)-immunoreactive myenteric neurons in the duodenum and ileum in diabetic rats. Forty rats were distributed into the following groups: normoglycemic (N), normoglycemic supplemented with vitamin C and vitamin E (NS), diabetic (D), and diabetic supplemented with vitamin C and vitamin E (DS). Vitamin C was added to the drinking water, and vitamin E was incorporated in the diet (1%). After 120 days, the animals were euthanized, and the duodenum and ileum were subjected to NADH-d and nNOS staining. Quantitative and morphometric analyses of myenteric neurons were performed. Diabetes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbic acid; Α-tocopherol; Diabetes mellitus; Myenteric plexus; Enteric nervous system.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400504

Nanofibrous Scaffolds for Tissue Engineering Applications
Provedor de dados: 52
Autores: Jaiswal,Amit.
The discipline of tissue engineering opens up the ways for repair and regenerate damaged organs and tissues. In the current work biomimetic nanofibrous scaffolds were fabricated by electrospinning. Poly-L-lactic acid (PLLA) was blended with collagen and gelatin to fabricate PLLLA-collagen and PLLA-gelatin fibrous scaffolds respectively. Pure PLLA and gelatin scaffolds served as controls. All the scaffolds displayed randomly oriented smooth fibers studied by SEM. Surface topography and roughness were studied by AFM and surface contact angle was also measured for all the fabricated scaffolds. Surface roughness was found to be higher in collagen and gelatin blended scaffolds in comparison to PLLA scaffold. Blending of collagen and gelatin reduced the surface...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrospinning; Biomimetic; Tissue Engineering.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100602

Genetic variability in four fish species (Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta) from Uruguay River basin
Provedor de dados: 52
Autores: Ramella,Micheline Sandra; Kroth,Mariela Aparecida; Meurer,Samira; Nuñer,Alex Pires de Oliveira; Zaniboni Filho,Evoy; Arisi,Ana Carolina Maisonnave.
The genetic variability of four fish species (Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta) collected in the upper Uruguay River basin was analyzed using the RAPD technique. A total of 118 amplified fragments was obtained, 11 for P. maculatus, 29 for P. lineatus, 45 for S. brasiliensis and 33 for S. scripta. Amplified fragments with monomorphic profile were not found in the studied species, except for S. brasiliensis, which presented seven monomorphic bands for Saltinho population. All species showed high levels of genetic variability among individuals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; Pimelodus maculatus; Prochilodus lineatus; Salminus brasiliensis; Steindachneridion scripta.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500008

Maize Productivity, Mycorrhizal Assessment, Chemical and Microbiological Soil Attributes Influenced by Maize-Forage Grasses Intercropping
Provedor de dados: 52
Autores: Méndez,Daniel Fernando Salas; Paula,Alessandra Monteiro de; Ramos,Maria Lucrécia Gerosa; Busato,Jader Galba.
Abstract Mycorrhizae are important components of agroecosystems and the diversification of crops stimulates the abundance of arbuscular mycorrhizal fungi and the participation of symbiosis in plant growth. This experiment examined mycorrhizal assessment, chemical and microbiological soil attributes in a maize-forage grasses intercropping compared to a maize-monocropping system. A complete randomized block design was used with crop systems installed under no-till management with three replicates, as follow: Maize (Zea mays L.); Panicum maximum Jacq. cv. Aruana; Urochloa humidicola (Rendle.) Schweickerdt.; Maize-P. maximum intercropping and Maize-U. humidicola intercropping. In 2015/2016 season, intercropping maize with Panicum maximum Jacq. cv. Aruana or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cropping systems; Arbuscular mycorrhiza; Tropical soils; Grain crops.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100205

Development of a Low Cost Bioprocess for Endotoxin Production by Bacillus thuringiensis var israelensis Intended for Biological Control of Aedes aegypti
Provedor de dados: 52
Autores: Soccol,Carlos Ricardo; Pollom,Teresinha E. V.; Fendrich,Ricardo Cancio; Prochmann,Fernando Alberto; Mohan,Radijiskumar; Blaskowski,Marita Maciel Moreira; Melo,André Luiz de Almeida; Carvalho,Cláudio Jose Barros de; Soccol,Vanete Thomaz.
Aedes aegypti is the vector of Dengue disease, responsible for 20,000 deaths/year worldwide. Bacillus thuringiensis var israelensis - Bti releases selective and effective toxins (crystal proteins) against A. aegypti larvae. We present a low cost bioprocess for toxin production, accomplished by a selected Brazilian strain Bti (BR-LPB01) and employment of low cost substrates. Soybean meal and sugarcane molasses lead to high toxic effectiveness after 2L bioreactor fermentation (LD50=26ng/mL), near to the reference strain IPS82 (LD50=17.3 ng/mL). The pH ranged between 5.8 and 7.0 during the exponential growth period and between 7.0 and 8.4 during the stationary phase, with low activity. Thus, control of foam and pH 7.0 were started and proved to be crucial for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopesticide; BTI; Dengue Fever Disease; Soybean meal; Sugarcane molasses.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700017

Bone-seeking therapeutic radiopharmaceuticals
Provedor de dados: 52
Autores: Srivastava,Suresh C.
Bone-seeking therapeutic radiopharmaceuticals are utilized on the basis of the radionuclide’s particulate emissions (primarily low to intermediate beta emission). The requirements therefore are different from those of bone imaging agents that consist mainly of short-lived single photon emitters. Lately, the therapeutic bone seeking radiopharmaceuticals have attained increasing importance due to their potential role in alleviating pain from osseous metastases in cancer patients, for the treatment of joint pain resulting from inflamed synovium (radiosynoviorthesis, or radiosynovectomy), or from various other forms of arthritic disease. There is, however, a paucity of published data on the bio-pharmacokinetics of these agents when used following intravenous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Therapeutic radiopharmaceuticals; Bone seekers; Pain palliation; Bio-pharmacokinetics; Radiosynovectomy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500007

Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor
Provedor de dados: 52
Autores: Porto,Ana Lúcia Figueiredo; Sarubbo,Leonie Asfora; Moreira,Keila Aparecida; Melo,Homero José Farias de; Lima-Filho,José Luís; Campos-Takaki,Galba Maria; Tambourgi,Elias Basile.
A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Efficiency; Mass transfer coefficient; Perforated Rotating Disc contactor; Aqueous two-phase system; Ascorbic oxidoreductase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019

Monitoring the nutritional and reproductive state of dairy cows through the presence of urea in milk
Provedor de dados: 52
Autores: Grande,Paula Adriana; Santos,Geraldo Tadeu dos; Ribeiro,Hilton; Damasceno,Júlio César; Alcalde,Claudete Regina; Barbosa,Orlando Rus; Horst,José Augusto; Santos,Fabio Seiji dos.
The effects of farm, parity (PO) and month of parturition on milk production, percentage of fat and protein, somatic cell count (SCC), and milk urea nitrogen (MUN) concentration were assessed on four specialized dairy farms using 120 cows. Also, the effects of MUN on gestation rate (GR) and artificial insemination rate (AIR) in early lactation were studied. The parameters of production and milk quality were similar among farms and were not influenced by the month of parturition. Farm D presented the highest MUN concentration. Concentration of MUN, percentages of fat and protein and SCC were not influenced by PO. Cows with a value of MUN between 10.1 and 13.0 mg/dL had the highest AIR and GR between days 55 and 70 postpartum and after 70 days in milk. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cattle; Gestation; Insemination; Milk urea nitrogen; Parturition order.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700032

Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum
Provedor de dados: 52
Autores: Zeni,Jamile; Gomes,Jonaína; Ambroszini,Éllin; Basso,Ana Paula; Toniazzo,Geciane; Valduga,Eunice.
Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A Plackett-Burman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL after 48 h of cultivation in a medium containing pectin (33.0 g/L), yeast extract (30.0 g/L) and potassium phosphate (2.0 g/L) at 30ºC, with a stirring rate of 180 rpm, initial pH 5.5 and 5x106spores/mL inoculum size. The kinetic evaluation in terms of substrate consumption demonstrated that the maximum production of PMGL was at 72 h, and 40% of the total organic carbon, 25% of the nitrogen, 88% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium brasilianum; Pectin liase; Experimental design technique.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600908

Effect of crude extract and its semi purified constituents from guaraná seeds [Paullinia cupana var. sorbilis (Mart.) lucke] on cognitive performance in Morris water maze in rats
Provedor de dados: 52
Autores: Otobone,Fernanda Jacques; Sanches,Andréia Conegero; Nagae,Rosangela Lika; Martins,Juliana Vanessa Colombo; Obici,Simoni; Mello,João Carlos Palazzo de; Audi,Elisabeth Aparecida.
The effect of crude lyophilized extract (EBPC) and the semi-purified constituents (EPA and EPB) of Paullinia cupana (guaraná) seeds long-term administered in rats by gavage at different doses was studied on cognitive behavior in rats. EBPC (30.0 mg/kg) and EPA (2.0 mg/kg), but not EPB (2.0 or 4.0 mg/kg) showed a smaller escape latency to find the emerged platform in Morris water maze test (MWMT), showing nootropic-like effect in normal rats, and in scopolamine induced amnesia rats compared to their controls (saline + 0.2% Tween 80) group. These extracts had no significant effect in open field test (OFT). Caffeine did alter escape latency in MWMT only in scopolamine induced amnesia rats and increased the crossings number in OFT, showing significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cognitive effects; Guaraná; Morris water maze; Paullinia cupana.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600007

Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid
Provedor de dados: 52
Autores: Pereira,Marcia de Oliveira; Rocha,Gabrielle de Souza; Lombardi,Simone dos Santos; Geller,Mauro; Pereira,Mário José; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Blood; Morphology; Plasmid; Fenoprofen.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700022

Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles
Provedor de dados: 52
Autores: Leitão,Cintia Camurça Fernandes; Costa,José Jackson do Nascimento; Brito,Ivina Rocha; Magalhães-Padilha,Déborah de Melo; Almeida,Anderson Pinto; Figueiredo,José Ricardo de; van den Hurk,Robert; Silva,José Roberto Viana.
The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At the beginning and end of culture, the follicular diameter was evaluated and compared. The levels of mRNA for GDF-9, FSH-R and BMPs -2, -4, -6, -7 and -15 in cultured follicles were quantified by real time PCR. The results showed that a significant increase of follicle diameter after six days when compared to day 0, but the presence of GDF-9 and FSH did not influence the follicular growth in comparison...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BMPs; Follicles; GDF-9; In vitro; MRNA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200007

Tropical Fruit Pulps: Processing, Product Standardization and Main Control Parameters for Quality Assurance
Provedor de dados: 52
Autores: Silva,Carlos Eduardo de Farias; Abud,Ana Karla de Souza.
ABSTRACT Fruit pulp is the most basic food product obtained from fresh fruit processing. Fruit pulps can be cold stored for long periods of time, but they also can be used to fabricate juices, ice creams, sweets, jellies and yogurts. The exploitation of tropical fruits has leveraged the entire Brazilian fruit pulp sector due mainly to the high acceptance of their organoleptic properties and remarkable nutritional facts. However, several works published in the last decades have pointed out unfavorable conditions regarding the consumption of tropical fruit pulps. This negative scenario has been associated with unsatisfactory physico-chemical and microbiological parameters of fruits pulps as outcomes of little knowledge and improper management within the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Processing; Standards of identity and quality; Vegetable product; Food safety.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100502

Ovary maturation stages and oocyte features in three species of the neotropical fish Hemiodus (Müller, 1842)
Provedor de dados: 52
Autores: Brandão,Claudia Angelica da Silva; Valentim,Maria de Fátima Moraes; Caramaschi,Érica Pellegrini.
Macro- and microscopic ovarian features of Hemiodus microlepis, H. ternetzi and H. unimaculatus were analyzed. Based on these features we proposed an ovarian maturation scale. The nine stages of the preliminary macroscopic fieldwork scale were reduced to five after microscopic analysis of ovaries. The microscopic analysis indicated a group-synchronous oocyte development common to the three species that were characterized as iteroparous synchronous spawners with a total spawning type. The remarkable thickness of the zona radiata layer and the large size of vitellogenic oocytes of Hemiodus ternetzi distinguished this species from the others.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemiodontid; Oocyte development; Zona radiata; Fish reproduction; Tocantins River.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300015

The Role of Apollon Gene Silencing on Viablity and Radiosensitivity of Cervical Cancer Hela Cells
Provedor de dados: 52
Autores: Milani,Saeideh; Bandehpour,Mojgan; Sharifi,Zohreh; Kazemi,Bahram.
Cervical cancer is the second common cause of cancer deaths in women worldwide. Radioresistancy of cancer is a principal cause of treatment impairing. Inhibitor of apoptosis proteins (IAPs) widely block apoptosis against apoptotic stimuli, including current chemo- and radiation therapies. Apollon, a membrane of IAP, can support cells against apoptosis and is over expressed in some treatment-resistant cancer cells. The aim of this study was to evaluate the effects of apollon knockdown on induction of apoptosis and also its potential for enhancement of radiosensitvity on hela cells. plasmid encoding shRNA which has been confirmed its effect against apollon, transfected into hela cells. Consequent effects on the level of P53 , Bax and BAK analyzed by real...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apollon; Apoptosis; Radiosensitivity; Cervical cancer.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100320

Dosimetric evaluation in radiation synovectomy
Provedor de dados: 52
Autores: Lima,Carla Flávia de; Campo,Tarcisio Passos Ribeiro de.
Rheumatoid arthritis can manifest itself through synovitis, of which the knee is the common locale. The treatment using an intra-articular radioisotope injection has been applied in various countries. In this work, the dose of radioactive material absorbed in the joint is evaluated, taking into consideration the dose received in the articular cartilage and adjacencies using a three-dimensional voxel model representing the knee. The radioisotopes studied were Samarium-153 and Dysprosium-165. The results show that the synovial membrane receives 85 to 98% of the normalized dose taken from all voxels representative of the synovium. The following features of 153Sm and of 165Dy - its short physical half-life, the gamma emissions with low energy which allow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; Radiation synovectomy; Dose absorbed.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700022

Cloning and Characterization of the Gene Encoding 3-hydroxy-3- Methylglutaryl-coenzyme A (HMG-CoA) Reductase from Fritillaria Cirrhosa D. Don
Provedor de dados: 52
Autores: Zhao,Qi; Li,Rui; Chen,Xiao; Yang,Qian; Li,Jian.
ABSTRACT The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in Mevalonate (MVA) pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR from Fritillaria cirrhosa (FcHMGR), a bulbous medicinal plant. The full-length cDNA of FcHMGR was 2072 base pair (bp), containing a 1680-bp open reading frame. Bioinformatical analyses revealed that FcHMGR had HMG CoA-binding domains and two NADPH binding domains, which are required for HMGR activity. Quantitative real-time PCR (qRT-PCR) analysis revealed that FcHMGR expressed high in mature bulbs. A truncated version of FcHMGR protein lacking the N-terminal 249-bp GC rich area was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 3-Hydroxy-3-methylglutaryl-CoA reductases; Fritillaria cirrhosa; Molecular cloning; Expression pattern; Prokaryotic expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100438

Absence of antidiabetic and hypolipidemic effect of Gymnema sylvestre in non-diabetic and alloxan-diabetic rats
Provedor de dados: 52
Autores: Galletto,Ricardo; Siqueira,Vera Lúcia Dias; Ferreira,Edilene Bega; Oliveira,Arildo José Braz; Bazotte,Roberto Barbosa.
In this study we investigated the antidiabetic and hypolipidemic potential of dried powdered leaves of Gymnema sylvestre (GS). The acute effect of GS administered by oral gavage on glucose blood level of and lipids in non-diabetic and alloxan-diabetic rats were investigated in the following conditions: a) after a balanced meal; b) after the ingestion of 1000 mg/kg amylose or 1000 mg/kg glucose; c) after the ingestion of a mixture of 12 mL/kg soybean oil + 1% cholesterol (SOC). In addition, the effect of the treatment with GS during two (sub-acute) or four weeks (chronic) on body weight, food and water ingestion, glucose blood level and lipids in non-diabetic and alloxan-diabetic rats were measured. The dose of GS utilized in the majority of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gymnema sylvestre; Herbal therapies; Diabetes; Asclepideceae.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400007

Morphological discrimination in juveniles of two Selene Species (Teleostei: Carangidae) using truss net distances
Provedor de dados: 52
Autores: Lima Filho,José de Melo; Lessa,Rosângela; Stosic,Borko; Duarte Neto,Paulo José; Vieira,José Wilson.
External morphological distinction between young Selene vomer and S. brownii (Carangidae) was studied using multivariate analysis with the purpose to evaluate if other characters than the second dorsal and pelvic fin spines could be used as distinguishing attributes for juveniles fishes. These spines are important distinctive morphological characters, but they can be absent in juvenile fishes or be broken during manipulation. A sample of 389 specimens was collected from beach netting at Caiçara do Norte (RN), northeastern Brazil, in 2001. Each specimen was digitalized and their images provided numerical matrices using Scion Image and FANTOMAS software. A C-language program was developed and used to calculate 16 Euclidean distances and nine homologous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Selene; Carangidae; Morphometric discrimination; Truss net.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300008

Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate
Provedor de dados: 52
Autores: Zhang,Qiaoyan; Qian,Junqing; Ma,Lingzhi.
This work aimed to obtain an ideal mutant strain with higher lipase yield using hydrazine hydrate (HZH) as a novel additive to treat Flavobacterium sp. strain YY25 by supercritical CO2. The survival rate and the positive mutation rate of the tested strain were strongly dependent on the dose of HZH. The treatment by 0.5% HZH in supercritical CO2 (8 MPa, 35°C) for 30 min provided 58.3% of positive mutation rate and an expected mutant strain with about 76.7% increase in lipase yield compared with the wild strain. Possible mutagenesis mechanisms were further explored. The analysis on pH drop of the treated seed liquid was performed to better understand the interaction. Results proved that the induced mutation with enhanced yield of lipase could be achieved by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Supercritical CO2; Mutation; PH Drop; Lipase; Flavobacterium sp.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400003

Mortality of Kalliapseudes schubartii in unvegetated soft bottoms of the estuarine region of the Lagoa dos Patos
Provedor de dados: 52
Autores: Fonseca,Duane Barros; D'Incao,Fernando.
Studies were carried iut on seasonal mortality by taking samples of Kalliapseudes schubartii (Crustacea: Tanaidacea) for a year in the estuarine region of the Lagoa dos Patos (southern Brazil). Results demonstrated no significant difference in seasonal mortality rates, which suggest that populations of K. schubartii was heavily preyed on year round and that an assumption of a significantly higher mortality in summer/autumn than in spring/winter might not be held in unvegetated soft bottoms of the estuarine regions of the Lagoa dos Patos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tanaidacea; Kalliapseudes schubartii; Mortality.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300011

Liming and postharvest quality of carambola fruits
Provedor de dados: 52
Autores: Prado,Renato de Mello; Natale,William; Corrêa,Márcio Cleber de Medeiros; Silva,José Antônio Alberto da.
To determine the effects of lime application on the postharvest quality of carambola fruit, an experiment with carambola trees cultivated on an acid soil was conducted at the Bebedouro, São Paulo, Brazil. The trees were treated with increasing doses of lime during the pre-planting period. Liming led to a increase in calcium concentration of carambola leaves and fruits. Adequate nutrition of the plant with calcium improved post-harvest fruit quality, permitting a longer (~ 2 days) period of storage under ambient conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca; Lime; Storage; Carambola averrhoa; Fruit quality.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600003

Interference of some organic substances and microorganisms adhered to stainless steel in ATP-bioluminescence measurement
Provedor de dados: 52
Autores: Simm,Erny Marcelo; Andrade,Nélio José de; Mendonça,Regina Célia Santos; Passos,Frederico José Vieira; Chaves,José Benício Paes.
Adhesion of organic substances and microorganisms on stainless steel surface was evaluated. The log RLU measurement was affected at a lower or higher degree, by type or concentration of the organic substances (casein, lipid, sucrose and their combinations) adhered to surface. However, if the samples analyzed were from the food processing surfaces, they would have been considered to be under satisfactory hygienic conditions with log RLU &lt; 2.18 (&lt; 150 RLU). The log RLU measurements of Staphylococcus carnosus-adhered surfaces, combined with or without the organic substances, were higher (p&lt;0.05) than those of the Bacillus subtilis spores-adhered surface, regardless of the concentration. The samples taken from the surfaces adhered with the casein,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hygiene monitoring; ATP-bioluminescence measurement; Adhesion on stainless steel.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300020

The occurrence of aflatoxin B1 contamination in peanuts and peanut products marketed in southern Brazil
Provedor de dados: 52
Autores: Hoeltz,Michele; Einloft,Tiago Centeno; Oldoni,Verônica Possebon; Dottori,Horacio Alberto; Noll,Isa Beatriz.
This study investigated the occurrence of aflatoxin B1 in peanuts and peanut products marketed in the State of Rio Grande do Sul, Brazil. One hundred one samples of peanuts and peanut products were collected and analyzed by thin-layer chromatography with a charge-coupled device system. Aflatoxin B1 was present in 14% of the samples analyzed, in concentrations ranging from 24.0 to 87.5 µg/kg in the peanut samples and from 22.0 to 84.6 µg/kg in the peanut-product samples. These values exceeded the Brazilian regulatory limit (20.0 µg/Kg for aflatoxins B1+G1+B2+G2 ). These results suggest that although aflatoxin contamination in peanuts marketed in southern Brazil is lower than in other Brazilian regions, it is still a serious problem for human health and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peanuts; Peanut products; Aflatoxin B1; Brazil.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200019

Impact of biocatalyst and moisture content on toluene/xylene mixture biofiltration
Provedor de dados: 52
Autores: Klapková,Eva; Halecký,Martin; Fitch,Mark; Soccol,Carlos Riccardo; Paca,Jan.
The objective of this work was to determine the influence of microbial inoculation on degradation efficiency. Three biofilters were used for the treatment of waste gas. A mixture of compost and perlite (8:2) served as the packing material. One biofilter was inoculated with a constructed microbial population. The second remained uninoculated, having the natural population present in the compost. The third biofilter was uninoculated and the packing material was sterilized. The degradation ability of the uninoculated biofilter started to drop after 18 days, while the removal efficiency of inoculated biofilter was stable. The sterile biofilter proved to have no removal efficiency. Moisture content of the packing and ability of the packing to keep moisture was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofiltration; Constructed mixed population; Moisture content; Pollutant mixture.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700018

Biogas and Biomass Implementation in Rural Areas
Provedor de dados: 52
Autores: Sandim,Álvaro A.; Batista,Alexandre G.; Bareta,Maria Luiza E.; Betini,Roberto C.; Pepplow,Luiz Amilton.
Abstract Advances in energy demand in rural areas cannot be dissociated from agricultural modernization, large estates, mechanization of labor and reduced investment in small production. The use of biogas together with biomass are the most cost-effective ways for the small producer, as it has a high calorific value. In the case of biogas the procedure is performed by combining a compressor and a chiller, allowing the gas enter the reactor and burn together with the air stream. The use of these techniques is an auxiliary way to reduce the cost of the producer to produce a certain crop, besides allowing a participation in the final energy supply, so that the utilities have an alternative to distribute the surplus energy to another region, serving thus a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biogas; Biomass; Rural areas; Electricity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200201

Soy Protein Isolate-Alginate Microspheres for Encapsulation ofEnterococcus faecalis HZNU P2
Provedor de dados: 52
Autores: Zhang,Yu; Zheng,Wei; Gu,Jian-Fei; Ni,Jian; Wang,Ling; Tang,Zhen-Xing; Shi,Lu-E.
ABSTRACTIn this work, the mixture of alginate and soy protein isolate used as a wall material was developed to encapsulateEnterococcus faecalis HZNU P2 (E. faecalis HZNU P2). The survival ability in the simulated gastric fluid (SGF) and bile salt solution, storage stability at different temperatures and release properties in the simulated intestinal fluid (SIF) of encapsulated cells were assessed. The results showed that encapsulation could offer sufficient protection toE. faecalis HZNU P2. The viability of encapsulatedE. faecalis HZNU P2 did not decrease in SGF at pH 2.5 or 2.0 after 2 h incubation, while free cells were reduced from 11 to 9.85 log CFU/mL in SGF (pH 2.5) at the same exposure time. Only minor viability of encapsulatedE. faecalis HZNU P2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus faecalis; Encapsulation; Soy protein isolate; Alginate; Stability; Viability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500805

Lymphoscintigraphy in breast cancer: a short review about the impact on upper limb after surgical treatment
Provedor de dados: 52
Autores: Bergmann,Anke; Resende,Juliana Miranda Dutra de; Santos-Filho,Sebastião David; Bello,Marcelo Adeodato; Oliveira,Juliana Flavia de; Palestro,Christopher J.; Bernardo-Filho,Mario.
Breast cancer is still associated with high mortality rates and one of the most important factors governing long survival is accurate and early diagnosis. In underdeveloped countries, this disease frequently is only detected in advanced stages; however, through mammography, many women have been diagnosed at early stages. In this context, the sentinel lymph node (SLN) technique is associated with less postoperative morbidity compared to axillary lymphadenectomy. Lymphoscintigraphy has emerged as a method for the evaluation of lymphatic drainage chains in various tumours, being both accurate and non invasive. The aim of this work is to present the main aspects which cause controversy about SLN and lymphoscintigraphy and the impact that these procedures have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Lymphoscintigraphy; Sentinel Lymph node Biopsy; Lymphedema.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700014

The fate of copepod populations in the Paranapanema River (São Paulo, Brazil), downstream from the Jurumirim dam
Provedor de dados: 52
Autores: Mitsuka,Patricia Maria; Henry,Raoul.
The longitudinal changes in the structure of copepod populations were examined during the dry and rainy seasons in a 42 km stretch of the Paranapanema River downstream from the dam of the Jurumirim Reservoir. Samples were taken in the "lacustrine" zone of the reservoir near the dam, and also at 12 stations distributed in the middle and the lateral regions of the channel of the Paranapanema River downstream from the dam. The following species of Cyclopoida were found at the sites: Thermocyclops decipiens, Thermocyclops minutus, Paracyclops sp., Tropocyclops sp and Mesocyclops sp., and of Calanoida: Argyrodiaptomus furcatus, Notodiaptomus iheringi and Notodiaptomus conifer. In the reservoir sampling station, the copepod abundance during the dry and rainy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Downstream; Reservoir; Copepod; Abundance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600012

Development of an L-Lysine Enriched Bran for Animal Nutrition via Submerged Fermentation by Corynebacterium glutamicum using Agroindustrial Substrates
Provedor de dados: 52
Autores: Letti Junior,Luiz Alberto; Letti,Gilberto Vinícius Melo; Soccol,Carlos Ricardo.
L-Lysine is an essential aminoacid added as supplement for animal feed. The aim of this work was to produce an L-Lysine enriched bran using Brazilian agroindustrial byproducts. Both the raw material costs and purification steps were minimized. Firstly, medium composition for the growth of Corynebacterium glutamicum ATCC 21799 was optimized targeting enhanced L-Lysine production - salt, vitamins and nitrogen sources concentrations were tested and selected. Next, UV mutant strains were generated and the best producers were used in formulated media using sugarcane molasses. It was reached a production of 9.3 g/L of L-Lysine with the optimized formulated media. This L-Lyisne rich broth was then impregnated and cyclically reimpregnated in pre-treated solid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-Lysine; Corynebacterium glutamicum; Submerged fermentation; Animal nutrition; Enriched bran.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100505

Molecular And 3D-Structural Characterization Of Fructose-1,6-Bisphosphate Aldolase Derived From Metroxylon Sagu
Provedor de dados: 52
Autores: Roslan,Hairul Azman; Hossain,Md. Anowar; Gerunsin,Jerry.
ABSTRACT Fructose-1,6-bisphosphate aldolase (FBAld) is an enzyme that catalyzes the cleavage of D-fructose-1,6-phosphate (FBP) to D-glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), and plays vital role in glycolysis and gluconeogenesis. However, molecular characterization and functional roles of FBAld remain unknown in sago palm. Here we report a modified CTAB-RNA extraction method was developed for the isolation of good quality RNA (RIN&gt;8) from sago leaves and the isolation of FBAld cDNA from sago palm. The isolated sago FBAld (msFBAld) cDNA has total length of 1288 bp with an open reading frame of 1020 bp and a predicted to encode for a protein of 340 amino acid resides. The predicted protein shared a high degree of homology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metroxylon sagu Fructose-1; 6-bisphospate aldolase 3D-structure stress Schiff's base.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100410

Azo Dye Mineralization by Phanerochaete Chysosporium in a Sequencing Bath Reactor
Provedor de dados: 52
Autores: Wanderley,Carlos Ronald Pessoa; Andrade,Marcus Vinícius; Pereira,Luciana José; Silva,Gloria Maria Marinho; Pessoa,Kelly Rodrigues.
ABSTRACT The mineralization of the azo dye congo red by the fungi Phanerochaete chrysosporium was studied in two sequential batch bioreactors (R1 and R2), operated in cycles of 48 h (step I) and 24 h (step II). In step I, glucose concentration was 1 g.L-1 in both reactors and in step II, 1 g.L-1 of glucose was maintained in R1, but R2 received no addition of glucose. In step I, the average dye removal efficiencies were 76 ± 29 % (R1) and 53 ± 15% (R2), while in step II the averages recorded for dye removal for R1 and R2 were 84 ± 15 and 70 ± 28%, respectively. The rates of dye removal were 0.04 h-1 in R1 and 0.03 h-1 in R2 in step I. Higher rates were obtained in step II, 0,07 h-1 and 0,02 h-1 for R1 and R2, respectively. The highest dye removal occurred...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Congo red; Phanerochaete chrysosporium; Sequential bioreactors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100606

Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.
Provedor de dados: 52
Autores: Predes,Fabrícia Souza; Matta,Sérgio Luis Pinto da; Monteiro,Juliana Castro; Oliveira,Tânia Toledo de.
This study was carried out to evaluate the effects of Arctium lappa L. (burdock) on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyl transferase (GGT), total protein, total cholesterol, urea, uric acid, triacylglycerol, calcium, phosphorus, chlorine and direct bilirubin. The morphological analysis did not reveal histopathological alterations in liver tissue. Both biochemical and morphological data did not indicate A. lappa toxicity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arctium lappa; Burdock; Plasma components; Liver.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200010

Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
Provedor de dados: 52
Autores: Priolli,Regina Helena Geribello; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Bajay,Miklos Maximiliano; Vello,Natal Antonio.
In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coefficient of parentage; Glycine max (L.) Merr.; Microsatellite markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004

Grain yield stability of wheat genotypes under irrigated and non-irrigated conditions
Provedor de dados: 52
Autores: Okuyama,Lauro Akio; Federizzi,Luiz Carlos; Barbosa Neto,José Fernandes.
Analyses of genotype by environment interactions were carried out for wheat genotypes grown under irrigated and non-irrigated field conditions, in two sowing dates during three years. The linear regression method was used to analyze the stability of grain yield. Genotype by environment interactions was observed for grain yield in all groups of environments. Nesser was the most productive and stable genotype in all groups of environments, followed by IAPAR 17-Caeté. In the late sowing, better yield performances were observed for the cultivars OCEPAR 7-Batuíra, IAPAR 17-Caeté and Nesser, under irrigated condition, and Nesser and IAPAR 28-Igapó, under non-irrigated condition. The results indicated that Nesser gave high grain yield with superior adaptability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drought tolerance; Genotype by environment interaction; Water stress.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600004

Germination, carbohydrate composition and vigor of cryopreserved Caesalpinia echinata seeds
Provedor de dados: 52
Autores: Zanotti,Rafael Fonsêca; Motta,Ludymila Brandão; Bragatto,Juliano; Labate,Carlos Alberto; Salomão,Antonieta Nassif; Vendrame,Wagner A.; Cuzzuol,Geraldo Rogerio Faustini.
The present study investigated the germination and vigor of Caesalpinia echinata (Brazilwood) seeds stored at negative temperatures. Recently harvested seeds were cryopreserved at -18º or -196ºC and periodically evaluated for germination, seed vigor and carbohydrate composition. The temperatures did not influence the germination percentages or vigor. The germination percentage decreased from 88% in recently harvested seeds to 60% after 730 days of storage. The different temperature and storage times tested did not affect the vigor seed germination as indicated by the measures of plant growth and survival. The different temperatures used did not cause changes in the carbohydrate composition. The tegument cell walls were rich in lignin, arabinose and xylose....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilwood; Carbohydrate; Conservation; Germination; Seed bank; Storage.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500004

Influence of post harvest processing conditions on yield and quality of ground turmeric (Curcuma longa L.)
Provedor de dados: 52
Autores: Bambirra,Maria Lúcia A.; Junqueira,Roberto G.; Glória,Maria Beatriz A.
Studies were carried out to evaluate the influence of post harvest processing conditions on yield and quality of ground turmeric. Rhizomes were peeled, cooked (autoclave or immersion) in water or alkaline media, sliced, dehydrated, ground, sieved, packaged in polyethylene bags and stored for 60 days at room temperature. Yields ranged from 9.84 to 14.51 g of powder/100 g of rhizome with moisture varying from 8.84 to 9.86 g/100 g. Peel removal caused 30% mass loss but the powder obtained had higher intensity of yellow and red. Cooking caused a reduction in dehydration time and provided a powder with lower moisture content, higher levels of curcuminoid pigments and higher Hunter CIE L*, a* and b* values. Cooking by immersion provided higher quality powder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turmeric; Curcuminoid pigments; Natural colorants; Processing.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600004

Effect of an aqueous extract of annatto (Bixa orellana) seeds on lipid profile and biochemical markers of renal and hepatic function in hipercholesterolemic rats
Provedor de dados: 52
Autores: Paula,Heberth de; Pedrosa,Maria Lúcia; Rossoni Júnior,Joamyr Victor; Haraguchi,Fabiano Kenji; Santos,Rinaldo Cardoso dos; Silva,Marcelo Eustáquio.
Annatto extract is a natural food color obtained from the outer coatings of the seeds of the Annatto tree (Bixa orellana L.). This is the first report in the literature that shows the relationship between the aqueous annatto extract and its influence on lipid profile in animals. Male Fisher rats were divided into three groups (n=12): C group, fed standard diet and water; H group, fed high-lipid diet and water and; HU group, with high-lipid diet and aqueous annatto extract for 60 days. The treatment with annatto extract in animals fed with the high-lipid diet lowered the LDL- and total cholesterol and raised the HDL-cholesterol, suggesting a hypocholesterolemic effect. Neither high-fat diet nor aqueous annatto extract had any significant effect on serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annatto; Bixa orellana; Cholesterol; Rat; Aqueous extract; Lipid profile.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600008

Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil
Provedor de dados: 52
Autores: Scodro,Regiane Bertin de Lima; Reinhold-Castro,Kárin Rosi; Dias-Sversutti,Alessandra de Cassia; Neitzke-Abreu,Herintha Coeto; Membrive,Norberto de Assis; Kühl,João Balduíno; Silveira,Thaís Gomes Verzignassi; Teodoro,Ueslei.
The purpose of this work was to verify the occurrence of Leishmania in naturally infected sandflies. The insects were collected with Falcão, Shannon and HP light-traps, in Doutor Camargo and Maringá municipalities between November 2004 and October 2005. Of the 11,033 sandflies collected in Doutor Camargo, 2,133 surviving females were dissected, particularly those of the Nyssomyia neivai species (86.87%). In Maringá, 136 sandflies were collected, of which 79 N. whitmani females and 1 Migonemyia migonei female were dissected. The dissected insects were identified and stored in the pools of 10 specimens. The PCR was carried out on 1,190 females of N. neivai and 190 of N. whitmani from Doutor Camargo, and on 30 of N. whitmani from Maringá, using the primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Sandflies; Dissection; Polymerase chain reaction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300007

Production of pre-basic potato seed by polyvinyl chloride PVC: articulate gutters hydroponic system
Provedor de dados: 52
Autores: Scherwinski-Pereira,Jonny Everson; Medeiros,Carlos Alberto Barbosa; Fortes,Gerson Renan de Luces; Pereira,Arione da Silva.
The development of more efficient and productive systems for pre-basic seed potato production would improve the quality of the propagative material used by the potato growers, directly affecting the crop yields. A two-year experiment was carried out to evaluate the potato pre-basic seed production by two types of hydroponic systems (fibrocement tiles and articulated PVC gutters), two cultivars (`Baronesa` and `Eliza`) and two types of propagative material (plants coming from in vitro culture and minitubers). The PVC gutters system was highly efficient. When using minitubers, this system reached multiplication rates up to 74 tubers per plant. Minitubers were more productive than in vitro plants, independent of cultivar and hydroponic system utilized.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Soilless culture; Multiplication; Propagation; Minitubers; Micropropagation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500007

Evaluation of nutrient supplementation to charcoal-treated and untreated rice straw hydrolysate for xylitol production by Candida guilliermondii
Provedor de dados: 52
Autores: Mussatto,Solange Inês; Roberto,Inês Conceição.
Xylitol was produced by Candida guilliermondii from charcoal-treated and untreated rice straw hemicellulosic hydrolysate with or without nutrients (ammonium sulphate, calcium chloride, rice bran extract). Both, xylitol yield and volumetric productivity decreased significantly when the nutrients were added to treated and untreated hydrolysates. In the treated hydrolysate, the efficiency of xylose conversion to xylitol was 79% when the nutrients were omitted. The results demonstrated that rice straw hemicellulosic hydrolysate treated with activated charcoal was a cheap source of xylose and other nutrients for xylitol production by C. guilliermondii. The non-necessity of adding nutrients to the hydrolysate media would be very advantageous since the process...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice straw hydrolysate; Nutrient; Activated charcoal; Candida guilliermondii; Xylitol.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300020

Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
Provedor de dados: 52
Autores: Rodrigues,Lia Rosane; Forte,Bianca de Camargo; Bodanese-Zanettini,Maria Helena.
In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androgenesis; Glycine max; Microspore culture; Microspore embryogenesis; Microspore isolation; Soybean.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002

Respirometry kinetics of phenol oxidation by Comamonas testosteroni Pb50 under various conditions of nutritional stress
Provedor de dados: 52
Autores: Paca,Jan; Kosteckova,Alena; Pacova,Leona; Prell,Ales; Halecky,Martin; Paca Jr.,Jan; Stiborova,Marie; Kozliak,Evguenii; Soccol,Carlos Ricardo.
Kinetics of phenol biodegradation using suspended biomass of Comamonas testosteroni Pb50 (monoculture) was measured under conditions of nutrient abundance, limitation, and prolonged cell starvation in a fed-batch reactor, with phenol being the sole carbon and energy source. The pre-washed cells were applied for measurement of the phenol and oxygen uptake rates at varied starting phenol concentrations with the kinetic parameters calculated using the Haldane model. The results revealed that nutrient limitation significantly suppressed the maximum value of exogenous respiration rate while the endogenous respiration rate, affinity and tolerance to phenol increased. By contrast, cell starvation resulted in a drop of both the exogenous and endogenous respiration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Respirometry; Kinetics; Phenol; Nutrition stress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600030

Potential of Tyrophagus putrescentiae (Schrank) (Astigmata: Acaridae) for the Biological Control of Lasioderma serricorne (F.) (Coleoptera: Anobiidae)
Provedor de dados: 52
Autores: Canevari,Glauco da Cruz; Rezende,Fernanda; Silva,Rafael Braga da; Faroni,Lêda Rita D'Antonino; Zanuncio,José Cola; Papadopoulou,Smaro; Serrão,José Eduardo.
This work investigated the potential of Tyrophagus putrescentiae (Schrank) (Astigmata: Acaridae) to control Lasioderma serricorne (F.) (Coleoptera: Anobiidae) in the laboratory. L. serricorne is the most destructive insect pest ever found on stored tobacco. The experimental delineation had 33 arenas being each experimental unit with 11 arenas of experiments with eggs, larva of L. serricorne with the density of T. putrescentiae maintained constant, where from 11 of these arenas, eight were for the analysis of predation and three to evaluate the natural mortality of the insect. The highest predation rate was found during the larval stage with, approximately, 54, 68 and 78% mortality of L. serricorne from the fourth until the sixty day of predation. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Lasioderma serricorne; Predators; Stored products; Tyrophagus putrescentiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200017

Zooplankton community composition and some limnological aspects of an oxbow lake of the Paraopeba River, São Francisco River Basin, Minas Gerais, Brazil
Provedor de dados: 52
Autores: Sampaio,Edson Vieira; López,Cristiane Machado.
Some limnological aspects of Lake Massacará (adjoining the Paraopeba River, São Francisco River Basin) were studied during two periods of the hydrological cycle. Water transparency was equally low during both the periods. During high water, thermal stratification was recorded, with a clinograde oxygen profile; pH was slightly acid, and conductivity was higher near the bottom. Concentrations of inorganic carbon and ammonia, and rates of primary production were higher than during the low water period. During the latter period, complete mixing occurred, with a clinograde oxygen profile; pH was slightly acid, and conductivity unvarying with depth. Higher concentrations of nitrate were found than during high water. With regard to the zooplankton communities,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxbow lake; Limnology; Zooplankton; Paraopeba River; São Francisco River Basin; Brazil.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300007

Micropropagation of Alibertia edulis Rich
Provedor de dados: 52
Autores: Silva,Fernanda Augusta Boato da; Pereira,Luiz Alfredo Rodrigues; Silveira,Conceição Eneida dos Santos.
The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA) alone or combined with indole-3-butyric acid (IBA) was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Alibertia edulis; Rooting; Germination; Activated charcoal.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600004

Studies on probiotics properties of two lactobacillus strains
Provedor de dados: 52
Autores: Brizuela,María Antonieta; Serrano,Paulina; Pérez,Yovanka.
"In vitro" studies were carried out for the selection of Lactobacillus strains with probiotics characteristics. Two strains of Lactobacillus were evaluated for the growth specific rate, generation time, acids, bile and antimicrobial resistances. "In vitro" tests indicated that the strains presented a potential for being used as probiotics.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotics; Lactobacillus; Selection.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100013

Genetic Diversity among Flue-cured Tobacco Cultivars Based on RAPD and AFLP Markers
Provedor de dados: 52
Autores: Zhang,Han Yao; Liu,Xiao Zhen; He,Chuan Sheng; Yang,Yu Ming.
The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cluster analysis (UPGMA) based on RAPD data and AFLP data. The accessions were grouped into three major clusters and these shared common ancestry clustered together.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flue-cured tobacco; RAPD; AFLP; Genetic diversity; DNA polymorphism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600003

Free radical scavenging activity of ethanolic extracts from herbs and spices commercialized in Brazil
Provedor de dados: 52
Autores: Mariutti,Lilian Regina Barros; Barreto,Gisela Pizarro de Mattos; Bragagnolo,Neura; Mercadante,Adriana Zerlotti.
Ethanolic extracts from 23 different dried herbs and spices commercialized in Brazil were investigated for their free radical scavenging properties using the stable free radicals 2,2'-diphenyl-β-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS•+), and Trolox as reference (TEAC) for both radicals. The kinetic curves for both radicals showed to follow the first order kinetics model and the decay rate constant (k obs) was calculated. For all the samples, the two methods showed a good linear TEAC correlation, indicating that the average reactivity of the compounds present in the ethanolic extracts was similar. Sage and rosemary extracts showed the highest free radical scavenging capacities, while onion showed negligible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant activity; Free radical scavenging activity; DPPH; ABTS; Herbs; Spices; Colorifico.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600018

Effect of oligofructose-enriched inulin on bone metabolism in girls with low calcium intakes
Provedor de dados: 52
Autores: Souza,Maria Cristina Corrêa de; Lajolo,Franco Maria; Martini,Ligia de Araujo; Correa,Nelton Bespalez; Dan,Milana Cara; Menezes,Elizabete Wenzel de.
In the present clinical study, the effect of oligofructose-enriched inulin was studied on bone metabolism in girls from 9 to 12 years old, with low habitual calcium intakes, who attended public schools. Two calcium-enriched formulations, supplemented with oligofructose-enriched inulin (test drink) or without (standard drink) were made. Sixty pre-pubertal girls were randomized into a double-blind and crossover design, divided into three groups and received one daily portion of either the standard drink (group 1) or test drink (group 2) during 11 weeks, followed by a three-week washout period. Group control did not receive any supplementation. Biochemical evaluations of serum calcium, intact parathyroid hormone - iPTH - and bone alkaline phosphatase - BAP -...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oligofructose-enriched inulin; Calcium intakes; Bone markers; Prepubertal girls; Prebiotics; Bone and mineral metabolism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100024

Growth Characteristics Modeling of Mixed Culture of Bifidobacterium bifidum and Lactobacillus acidophilus using Response Surface Methodology and Artificial Neural Network
Provedor de dados: 52
Autores: Meena,Ganga Sahay; Majumdar,Gautam Chandra; Banerjee,Rintu; Kumar,Nitin; Meena,Pankaj Kumar.
Different culture conditions viz. additional carbon and nitrogen content, inoculum size and age, temperature and pH of the mixed culture of Bifidobacterium bifidum and Lactobacillus acidophilus were optimized using response surface methodology (RSM) and artificial neural network (ANN). Kinetic growth models were fitted for the cultivations using a Fractional Factorial (FF) design experiments for different variables. This novel concept of combining the optimization and modeling presented different optimal conditions for the mixture of B. bifidum and L. acidophilus growth from their one variable at-a-time (OVAT) optimization study. Through these statistical tools, the product yield (cell mass) of the mixture of B. bifidum and L. acidophilus was increased....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Response surface methodology (RSM); Artificial neural network (ANN); Genetic algorithms (GA); Fractional factorial design (FFD); Bifidobacterium bifidum; Lactobacillus acidophilus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600962

Evaluation of toxic effects with transition metal ions, EDTA, SBTI and acrylic polymers on Aedes aegypti (L., 1762) (Culicidae) and Artemia salina (Artemidae)
Provedor de dados: 52
Autores: Arruda,Eduardo José de; Rossi,Ana Paula Leão; Andrade Porto,Karla Rejane de; Oliveira,Lincoln Carlos Silva de; Arakaki,Andrea Haruko; Scheidt,Gessiel Newton; Roel,Antonia Railda.
This work aimed to evaluate the toxicity of some insecticides compounds on Aedes aegypti and Artemia salina larvae. Bioassays were carried out to evaluate the toxic effect after of 24 and 72 h using the compounds or associations. The LC10, LC50 and LC90 values were obtained and utilized for toxicity comparations. For Ae. aegypti, LC50 were 32.65 mg L-1 in 24 h for Na2[EDTA-Cu(II)] and total mortality in 72 h for SAP-Na2[EDTA-Cu(II)].
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue fever; Insect control; Lethal Concentration.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300010

Biodegradation of blend films PVA/PVC, PVA/PCL in soil and soil with landfill leachate
Provedor de dados: 52
Autores: Campos,Adriana de; Marconato,José Carlos; Martins-Franchetti,Sandra Mara.
This study investigated the biodegradation of blends films of poly(vinyl alcohol)/poly(vinyl chloride) (PVA/PVC) and poly(vinyl alcohol)/poly(caprolactone) (PVA/PCL) blends films prepared with dimethylformamide under a variety of conditions by respirometry, spectrophotometry (FTIR), scanning electron microscopy (SEM), and contact angle. The films were buried in the garden soil and in the soil mixed with the landfill leachate for 120 days at 28ºC. Significant levels of biodegradation were achieved in fairly short incubation times in the soil. The results indicated that PVA was the most biodegradable film in the soil and in the soil with the leachate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synthetic polymers; Biodegradable polymers; Soil; Poly/vinyl alcohol; Poly/caprolactone; Poly/vinyl chloride.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600024

Piper gaudichaudianum Kunth: Seasonal Characterization of the Essential Oil Chemical Composition of Leaves and Reproductive Organs
Provedor de dados: 52
Autores: Schindler,Bianca; Heinzmann,Berta Maria.
ABSTRACT This study describes a comparative analysis of the essential oil (EO) chemical composition of leaves and reproductive organs (inflorescences and fruits) of Piper gaudichaudianum during the seasons of a year in order to determine the best collection time and the most suitable plant organ to obtain this extractive. The chemical composition of EO obtained from fresh leaves was compared to the dried ones, to verify if the drying process interferes in the extractive quality. The leaves were collected from a native population of Santa Maria, RS, Brazil, twice in each season, in triplicate, while inflorescences and fruits were sampled when they were present. The EO was obtained by hydrodistillation of the different plant organs for 3 h. The 20 EO samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pariparoba; Plant extraction; Medicinal plant; Post-harvest processing.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100603

The Dynamic of some Biochemical Indicators at Species of Medicinal Plants from Areas of Suceava County, Romania, Emerged under the Mining Pollution Influence
Provedor de dados: 52
Autores: Todirascu-Ciornea,Elena; Dumitru,Gabriela.
ABSTRACT It is known the fact that the mining causes huge damages on the environment having negative biologic effects extremely severe on some groups of organisms of the food chain. The pollutants causes major damages at the level of leaves' cuticles, the vegetation reacting through irreversible modifications at the tissue's level and at substances and energy metabolism, like a riposte to the apparition of the stress induced by the ROS, stress that creates real disturbances on physiological and biochemical processes. Considering the fact that, on the one hand, the activity of oxidoreductases is strongly influenced by the presence of the pollutants factors, with direct effect on the oxidative's stress production, and on the other hand that the plants react...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Mint; Plantain; Pollution.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100344

Measuring of the Genotoxic and Potential Antioxidant Effects of Essential Oil Obtained from Satureja Hortensis Against to Phaseolus Vulgaris
Provedor de dados: 52
Autores: Bozari,Sedat.
ABSTRACT It is known that, there are many ways for increasing the yield at the crops specially cereal products. Genetic manipulations and physiological interventions are the primary studies that aim to obtain products at high quality and amounts. It is known that, the usage of synthetic chemicals for physiological inverventions negatively affects organism. But using natural plant products instead of these chemicals is a subject that draws attention of today scientific environment. In this study, this situation was taken into consideration and the essential oil obtained from Satureja hortensis (SEO) plant were applied to bean seeds. The genotoxic and physiological effects of SEO at four different dosages were detected against to Phaseolus vulgaris...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant Enzymes; Biyopesticide; Essential Oil; GTS.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100431

Resistance of gastrointestinal nematodes to anthelmintics in sheep (Ovis aries)
Provedor de dados: 52
Autores: Thomaz-Soccol,Vanete; Souza,Felipe Pohl de; Sotomaior,Cristina; Castro,Edilene Alcântara; Milczewski,Viviane; Mocelin,Giovani; Silva,Maria do Carmo Pessoa e.
The fecal egg count reduction test (FECRT) was used in a survey for anthelmintic resistance in 42 sheep farms, in five different regions in Paraná State, Brazil between July 1996 - July 2000. Animals with natural infection were tested. Five drugs were used individually or in combination - benzimidazole, imidazothiazole, ivermectin, milbemicina and closantel. Results showed that the prevalence of resistance to all anthelmintic was as high as 88.1% for benzimidazoles (oxfendazole), 78.6% for ivermectin, 56.4% for closantel, 38.7% for closantel + oxfendazole, 38% for levamisole, and 23.6% for moxidectin. There was multiple resistance in all tested farms. The most important infective larvae recovered from culture were Haemonchus contortus and Trichostrongylus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastrointestinal parasite; Sheep nematode; Ovis aries; Anthelmintic resistance; FECRT.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100006

Hermit crab (Decapoda, Anomura) attraction to dead gastropod baits in an infralittoral algae bank
Provedor de dados: 52
Autores: Pezzuti,Juarez C. B.; Turra,Alexander; Leite,Fosca P. P.
Hermit crabs use gastropod shells as shelter and are adapted to follow chemical cues released from tissues of dead or injured gastropods as a way to find new and more adequate shells. The species composition, crab size, shell types adequacy and physical condition were compared between attracted individuals and crabs collected in previous samples. The previous sampling was carried out in five areas before each experiment. Then, five baits of crushed gastropods in nylon net bags were installed in these areas. Three samples were taken at 30min intervals, capturing all crabs within a circle of 60cm diameter. Attraction of hermit crabs was tested for four different gastropod baits to verify specificity of the chemical cues. Clibanarius antillensis, Pagurus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hermit crab; Chemical attraction; Gastropod predation sites; Shell use; Shell condition.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200018

Analysis of the Operation of Photovoltaic Systems Installed at Federal University of Technology - Paraná in Curitiba
Provedor de dados: 52
Autores: Urbanetz Junior,Jair; Tonin,Fabianna Stumpf; Mariano,Juliana D’Angela; Tiepolo,Gerson Máximo.
ABSTRACT The Federal University of Technology - Paraná (UTFPR), Campus Curitiba, has a Grid-Connected Photovoltaic System (GCPVS) of 2.1 kWp in the Green Office (GO), which has been in operation since December 2011, and until September 2017 generated little more than 13.64 MWh and a GCPVS of 10.2 kWp, in operation since February 2016. The photovoltaic panel of the 2.1 kWp system was set up following the inclination of the roof of the building, which does not give its maximum performance. On the other hand, the panel of the 10.2 kWp system in Neoville was installed in optimum conditions, that is, oriented to the north and with slope equal to the latitude of Curitiba and, up tol September 2017 generated more than 20.65 MWh. This paper presents the monitoring...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solar energy; Distribuited generation; Grid-connected photovoltaic system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200237

Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa L.) and garlic (Allium sativum L.) extracts
Provedor de dados: 52
Autores: Benkeblia,Noureddine.
The radical scavenging activity (RAS), chain-breaking activity, H2O2-scavenging, reducing capacity and total phenolics of four types of onions (Green onion, Yellow, Red and Purple) and garlic were investigated. Total phenolics varied from 30 mg (green onion) to 49 mg.100 g-1 fresh weight (garlic). Garlic extract showed the highest RAS, while green onion showed the lowest one. The chain-breaking activity of green onion extract was higher (0.48) than garlic extract (0.029). Chain-breaking activity of yellow, red and purple onion extracts was 0.19, 0.048 and 0.032 respectively. However, heating treatment (90 ºC, 3h) caused an increase in this activity. Low ability of green onion extract to scavenge hydrogen peroxide was noted (35%), whereas high ability was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radical scavenging activity; Chain-breaking activity; H2O2-scavenging; Reducing capacity; Allium cepa; Allium sativum.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600011

Isolation of natural inhibitors of papain obtained from Carica papaya latex
Provedor de dados: 52
Autores: Monti,Rubens; Contiero,Jonas; Goulart,Antonio José.
Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esterase activity; Natural papain inhibitor; Protease inhibitor.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010

Galactans: an overview of their most important sourcing and applications as natural polysaccharides
Provedor de dados: 52
Autores: Delattre,Cedric; Fenoradosoa,Taratra Andrée; Michaud,Philippe.
Since last decades, lot of biological and rheological properties of polysaccharides and oligosaccharides were described. Among them, galactans and more especially sulfated galactans from seaweeds have shown interesting and specific properties not only as texturing agents but also as biological active compounds on several organisms. This class of polysaccharides includes classical sulfated galactans extracted from seaweeds and classified as agar and carrageenans. However, some galactans are more complex and their specific structural features have been characterized after their extraction from terrestrial plants, seaweeds but also animals and microoragnisms. This review catalogues the origins, structural characteristics and potentialities of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polysaccharide; Seaweed; Oligosaccharides; Galactan.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600002

Evaluation of gastric anti-ulcer activity in a hydro-ethanolic extract from Kielmeyera coriacea
Provedor de dados: 52
Autores: Goulart,Yara Cavalcante Fortes; Sela,Vania Ramos; Obici,Simoni; Martins,Juliana Vanessa Colombo; Otobone,Fernanda; Cortez,Diogenes Aparicio; Audi,Elisabeth Aparecida.
The antiulcer activity of a hydro-ethanolic extract prepared from the stems of Kielmeyera coriacea Mart. (Guttiferae) was evaluated in rats employing the ethanol-acid, acute stress and Indomethacin models to induce experimental gastric ulcers. Treatment with K coriacea hydro-ethanolic extract provided significant antiulcer protection in the ethanol-acid and Indomethacin models, but not in the acute stress model. These results suggested that the K coriacea hydro-ethanolic extract increased resistance to necrotizing agents, providing a direct, protective effect on the gastric mucosa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute stress; Anti-ulcer activity; Ethanol-acid; Indomethacin; Kielmeyera coriacea Mart. (Guttiferae).
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200007

Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting
Provedor de dados: 52
Autores: Ono,Elisabete Yurie Sataque; Fungaro,Maria Helena Pelegrinelli; Sofia,Silvia Helena; Miguel,Tatiana de Ávila; Sugiura,Yoshitsugu; Hirooka,Elisa Yoko.
In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB1 and FB2 with levels ranging from 2.41 to 3996.36 µg/g, and from 1.18 to 1209.91 µg/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289.84 µg/g), eight as intermediate (&gt;1289.84 to 3772.44 µg/g) and four strains as high (&gt;3772.44 µg/g) fumonisin producers. From the total of 105 loci amplified, 60 (57.14%) were polymorphic. RAPD analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. Although RAPD markers were capable of discriminating the different F....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fumonisin; Fusarium verticillioides; Genetic variability; RAPD.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400026

Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2
Provedor de dados: 52
Autores: Ferreira,Graziela Batista; Evangelista,Alex Ferreira; Severo Junior,João Baptista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile; Jordão,Elizabete.
This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partitioning; Optimization; Aqueous two-phase systems; Maize malt; PEG 6000; CaCl2.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300020

Isolation of bacteriocin - producing lactic acid bacteria from 'Ugba' and 'Okpiye', two locally fermented nigerian food condiments
Provedor de dados: 52
Autores: Nwuche,Charles Ogugua.
In this work, 100 samples each of 'ugba' and 'okpiye' were evaluated for the presence of bacteriocin producing lactic acid bacteria. Thirty strains showing antibacterial activity against at least one of the indicator organisms were selected from a total of 752 colonies isolated from the condiments. Out of the 30, only five strains retained activity after the pH of the broth supernatant was adjusted to 6.5. When evaluated by the agar-well diffusion assay, the spectra of inhibitory activity showed that Staphylococcus aureus was the most sensitive indicator organism tested, while Listeria monocytogenes was the most resistant. One strain (UG 2) was active against Escherichia coli. The assays using the cell-free supernatant of the cultures showed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 'Ugba'; 'Okpiye'; Lactic acid bacteria; Bacteriocins; Inhibitory activity; Condiments; Cell free supernatant.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100013

The effect of temperature, salinity and nitrogen products on food consumption of pink shrimp Farfantepenaeus paulensis
Provedor de dados: 52
Autores: Wasielesky Jr.,Wilson; Bianchini,Adalto; Castaño Sanchez,Cecilia.; Poersch,Luis Henrique.
Studies were carried out to investigate the effect of temperature, salinity, ammonia, nitrite and nitrate on food consumption of pink shrimp Farfantepenaeus paulensis. Juveniles (0.2 - 0.4 g) were acclimated for 15 days in seawater with different temperatures, salinities and concentrations of ammonia, nitrite and nitrate. After the acclimation period, 20 shrimps per treatment were individualized in order to have their ration intake analyzed through the amount of ration offered and left over within a 24-hour period. Mean food consumption presented significant alterations (P&lt;0.05) for the tested temperatures and nitrite concentrations, whereas for the salinity, ammonia and nitrate treatments, shrimp presented no alteration on food intake (P&gt;0.05)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture; Food consumption; Shrimp; Water quality.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100019

Functional response of the predator Eriopis connexa (Coleoptera: Coccinellidae) to different prey types
Provedor de dados: 52
Autores: Sarmento,Renato Almeida; Pallini,Angelo; Venzon,Madelaine; Souza,Og Francisco Fonseca de; Molina-Rugama,Adrián José; Oliveira,Claudinei Lima de.
This work evaluated the functional response of adult females of Eriopis connexa to different densities of Macrosiphum euphorbiae and Tetranychus evansi. When preying upon aphids, E. connexa presented a sigmoidal functional response (Type III). This behavior, however, changed drastically to an exponential (Type II) functional response, when mites (T. evansi), rather than aphids, were offered to E. connexa. Such different patterns showed that this coccinellid needed to adopt distinct strategies according to the kind of prey available. Since predators were believed to be able to regulate prey populations only when adopting Type III functional response. E. connexa would be a good candidate for a biological control agent of M. euphorbiae, but would not suppress...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Coccinellids; Aphids; Mites; Lycopersicon esculentum Mill.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100014

Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
Provedor de dados: 52
Autores: Guo,Lina.
ABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of AcerOr1 gene, we analyzed its expression and localization. The results showed that AcerOr1 mRNA was predominantly expressed in the antennae of nurse and forager bees, while the AcerOr1 protein was predominant in thorax, and its expression in antennae was higher in forager than in nurse. IHC revealed that AcerOr1 mainly localized in the olfactory neurons of the antennae. In addition, the staining intensity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis cerana cerana; Olfactory receptor; AcerOr1; Expression; Localization; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440

In Vitro Assessment of Antileishmanial Activity of Natamycin and Nystatin
Provedor de dados: 52
Autores: Sidana,Arushdeep; Negi,Ajeet Kumar; Farooq,Umar.
ABSTRACT The present study was aimed to evaluate the in vitro antileishmanial activity of four different concentrations of natamycin and nystatin by using MTT 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide reduction assay. In vitro antileishmanial activity revealed that the IC50 of natamycin (80.49 μg/ml) and nystatin (105.7 μg/ml) was less than that of sodium stibogluconate (127.9 μg/ml), and more than amphotericin B (18.91 μg/ml).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Leishmania donovani; Antileishmanial; Natamycin; Nystatin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100301

Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers
Provedor de dados: 52
Autores: Souza,Silvia Graciele Hülse de; Carpentieri-Pípolo,Valéria; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Ruas,Paulo Maurício; Gerage,Antônio Carlos.
The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn; Distance genetic; Microsatellites; Molecular markers; Polymorphism; RAPD.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100022

Shelf life of custard apple treated with 1-methylciclopropene: an antagonist to the ethylene action
Provedor de dados: 52
Autores: Benassi,Guilherme; Correa,Guilherme Augusto Simões Francischini; Kluge,Ricardo Alfredo; Jacomino,Angelo Pedro.
Custard apple (Annona squamosa L.) presents very short storage life at room temperature, in part due to heavy losses in firmness. This process is associated with the production and action of the hormone ethylene. In order to retard the ripening evolution in custard apple, fruits were treated with the competitive ethylene antagonist 1-methycyclopropene (1-MCP) at concentrations of 0, 30, 90, 270 or 810 nL L-1 for 12 h at 25ºC and then stored at 25ºC for four days. The soluble solids content (SSC), firmness and percentage of ripe fruits (firmness &lt; 0.5kg) were determined during the experimental period. There were no differences among treatments as to the SSC. Fruits treated with 810 nL L-1 of 1-MCP showed higher firmness than the control fruits. Both ,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ripening; Storage; Firmness; Annona squamosa.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100016

Effects of storage time and temperature on the characteristics of vegetable-type soybean grain minimally processed
Provedor de dados: 52
Autores: Czaikoski,Karina; Carrao-Panizzi,Mercedes Concordia; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko.
The objective of this study was to evaluate the effects of storage time and temperature on the characteristics of vegetable-type soybean grain (cultivar BRS 267) minimally processed and to define the best conditions for its storage. The evaluation was performed by measurement of vitamin C levels, weight loss and color parameters (L*, a*, and b*). The time of storage of vegetable-type soybean grains minimally processed and storage in Styrofoam trays and wrapped with PVC film, caused a decreased in vitamin C levels and color parameters and increased weight loss. This process was intensified with higher temperature at 25 °C than 5°C. To maintain appropriate levels of vitamin C, weight and color of vegetable-type soybean grains minimally processed and storage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetable-type soybean; Quality; Edamame.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400002

Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
Provedor de dados: 52
Autores: Wang,Yong; Huang,Wenhua; Peng,Daizhi; Hu,Yuanbin.
In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear transcription factor; Electrophoretic mobility shift assay; Methodology; Modification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025

Evaluation of Apis mellifera Carniolan and Africanized honey bees in royal jelly production
Provedor de dados: 52
Autores: Mouro,Gisele Fernanda; Toledo,Vagner de Alencar Arnaut de.
Investigations were carried out to evaluate Africanized and Carniolan hybrid Apis mellifera honeybees in royal jelly production. Ten colonies were randomly collected in nature and housed in Langstroth beehives. Each hive was having two nuclei with five frames. In superior nucleus frame was containing 28 acrylic cups in two bars. The five colonies with the best production remained in the experiment and their queens replaced by the respective daughters. Carniolan queens were placed in the five Africanized honeybees colonies with lower production. The hybrid Carniolan showed a higher production (P=0.0001) of royal jelly per colony, in three-day collect (3.95 ± 2.92 g) when compared to the Africanized honeybees (2.23 ± 2.13 g). The Carniolan also had a higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Royal jelly; Africanized honeybees; Carniolan; Hybrid honeybees.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300018

Extraction of essential oils from lime (Citrus latifolia Tanaka) by hydrodistillation and supercritical carbon dioxide
Provedor de dados: 52
Autores: Atti-Santos,Ana Cristina; Rossato,Marcelo; Serafini,Luciana Atti; Cassel,Eduardo; Moyna,Patrick.
In this work lime essential oils were extracted by hydrodistillation and supercritical carbon dioxide. In the case of hydrodistillation, the parameters evaluated were extraction time and characteristics of the plant material. In supercritical extraction, the parameters evaluated were temperature, pressure, CO2 flow, extraction time and material characteristics. Considering citral content, the best results for hydrodistillation were obtained with a distillation time of 3 hours using whole peels. The best results for supercritical extraction were found using 60ºC, 90 bar, at a CO2 flow rate of 1 mL/ min for 30 minutes using milled peels. The best yields of lime oil were obtained by hydrodistillation (5.45% w/w) and supercritical extraction (7.93% w/w) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus latifolia; Lime; D-limonene; Supercritical carbon dioxide; Hydrodistillation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100020

Study of gels of molybdenum with cerium in the preparation of generators of 99Mo - 99mTc
Provedor de dados: 52
Autores: Moraes,Vanessa; Marczewski,Bárbara; Dias,Carla Roberta; Osso Junior,João Alberto.
99mTc has ideal nuclear properties for organ imaging in nuclear medicine, and it is obtained from the 99Mo-99mTc generator. Four different types of generators are available: chromatographic that uses 99Mo from fission of uranium; MEK solvent extraction; Tc2O7 sublimation; gel chromatographic. This work presents the preparation of gel generators of molybdenum with cerium and characterization of the gels: mass ratio between molybdenum and cerium, structure, size of particles and elution percentage of 99mTc after irradiating the gels. Eight gels were prepared at the same temperature of 50 ºC with concentrations of NaOH of 2 and 4 mol/L, mass ratio of 0.31 and 0.38 and final pH of 3.5 and 4.5. The analysis of the results proved that these gels are not adequate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioisotopes; Technetium-99m; Molybdenum-99; Gel generator.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700007

Restructured fish product from white croacker (Micropogonias furnieri) mince using microbial transglutaminas
Provedor de dados: 52
Autores: Gonçalves,Alex Augusto; Passos,Marcelo Gonzalez.
This study aimed at determining the influence of three concentrations of commercial transglutaminase enzyme in restructured fillet of minced fish from white croacker (Micropogonias furnieri), one of the four marine species with notability in Brazil. The restructured fillet developed had advantages when compared to traditional fillet, such as absence of spine and less flavour intensity (washes cycles). Washing process for white croacker mince was compared with five clarification agents: water (control), phosphoric acid (H3PO4), sodium chloride (NaCl), calcium carbonate (CaCO3) and sodium bicarbonate (NaHCO3). The higher quality product (whiteness) was obtained with calcium carbonate washes. Three concentrations (1.5, 1.0 and 0.5%) of microbial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seafood; Minced fish; White croacker; Transglutaminase; Restructured fish.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400030

Production and Characterization of Bacteriocin Produced by Lactobacillus Viridescence(NICM 2167)
Provedor de dados: 52
Autores: KP,Sure; PV,Kotnis; PK,Bhagwat; RC,Ranveer; PB,Dandge; AK,Sahoo.
The present study focused on the production optimization of bacteriocin by Lactobacillus viridescence NICM 2167 followed by its purification and characterization. The bacteriocins are antimicrobial peptides produced by many Gram positive and Gram negative bacteria.The bacteriocin produced by LAB (lactic acid bacteria) received attention in recent years due to their potential application as natural preservatives in food. Bacteriocinproduced by Lactobacillus viridescence showed broad range of antimicrobial activity against food borne pathogens. Production parameters were optimized showing highest production of bacteriocinin MRS broth with pH= 7.0 incubated at 37°C for 48 h. Bacteriocin was purified in two steps involving ammonium sulphate precipitation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus viridescence; NICM 2167; Bacteriocin; Antimicrobial activity; Gel filtration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100407

Effects of light and temperature on seed germination in Cecropia hololeuca Miq. (Cecropiaceae)
Provedor de dados: 52
Autores: Godoi,Simone; Takaki,Massanori.
Seeds of Cecropia hololeuca were submitted to regimes of constant and alternating temperatures, associated with photoperiods of white light, "shade light" and continuous darkness under controlled conditions in laboratory. Seeds did not germinate at constant temperatures of 10°C to 20°C and above 35°C. Seeds only germinated in the darkness when the temperature was alternated. "Shade light" inhibited germination. We concluded that the regime of alternating temperatures suppressed the light effects on seed germination of this species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cecropia hololeuca; Germination; Alternating temperature; Phytochrome; Light.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200004

Electrolytic treatment applied to the industrial effluent containing persistent wastes monitored by Bartha respirometric assays
Provedor de dados: 52
Autores: Régis,Gisela; Bidoia,Ederio Dino.
The effluent of a rubber chemical antioxidant and antiozonant producer industry, with high content of organic material was subjected to electrolytic process. To evaluate the speed of stabilization of the eletroctrolyzed effluents, and to evaluate the biodegradation the respirometric test of Bartha and Pramer was used. The monitoring of the biodegradation of the effluent, after different periods of electrolysis show that the ideal time of electrolysis was 10 and 25 min. It was concluded that the eletrolytic process was viable to diminish the adaptation time of the microorganism to the effluent and consequently increased the biodegradation of effluent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrolytic process; Biodegradation; Wastes pollutant; Electrodes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200020

Editorial
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200001

Population Changes of Eubacteria, Sulfate-Reducing Bacteria and Methanogenic Archaea in an Anaerobic Reactor Processing Ethanol Distillery Vinasse
Provedor de dados: 52
Autores: Brown,Adis Ivonne Terry; Pozzi,Eloisa; Damianovic,Marcia Helena Rissato Zamariolli; Briones,Homero Enrique Urrutia; Ortiz,Leslie Ester Abarzúa; Pires,Eduardo Cleto.
Abstract The microbiological characterization by molecular techniques (DGGE and quantitative PCR) of Archaea, Bacteria domain and sulfate-reducing bacteria (SRB) from a laboratory scale Up-flow Anaerobic Sludge Blanket (UASB) processing sugar cane vinasse was performed during the operational phase with increasing organic loads. The organic load removal efficiency was between 97% and 75% for volumetric organic loads (VOL) in the range of 0.6 to 15.4 kgCOD.m-3.d−1 and for higher VOL (until 27.0 kgCOD.m-3 .d−1) the removal efficiency decreased to 48%. Archaea represented the majority of the estimated population (107 copies of 16S RNA ribosomal gene. mL-1) followed by bacteria (106 copies of 16S RNA ribosomal gene. mL-1) and sulfate-reducing bacteria (SRB)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: UASB reactor; Microbial characterization; Methanogenesis; Sulfidogenesis; DGGE; Quantitative PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100203

Comparison of biostimulation and bioaugmentation techniques for the remediation of used motor oil contaminated soil
Provedor de dados: 52
Autores: Abdulsalam,Surajudeen; Omale,Adaji Benjamin.
This study was carried out on the bioremediation of used motor oil contaminated soil artificially contaminated to a pollutant level of 40,000ppm using biostimulation and bioaugmentation remediation techniques for 42 days. Four treatment options were investigated in wooden microcosms: Control (T1), water amended (T2), biostimulation (T3) and hybrid of biostimulation and bioaugmentation (T4). The effectiveness of bioremediation processes were monitored using the total petroleum hydrocarbon removal (TPH) and total bacterial count (TBC). T3 had the highest TPH removal rate (69.2±0.05%), followed by T4 (65.2±0.25%) and T2 (58.4±0.5%); the control (T1) had the lowest TPH removal rate (43.2±1.5%). TBC revealed that bioremediation actually took place; T4 had the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioremediation; Biostimulation; Bioaugmentation; TPH; Used motor oil; Wooden microcosms.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300027

Benthic Community zonation in a breakwater on the north coast of the state of Rio de Janeiro, Brazil
Provedor de dados: 52
Autores: Masi,Bruno Pereira; Macedo,Isabela Maria; Zalmon,Ilana Rosental.
The present study presents the vertical zonation pattern of the intertidal benthic organisms on granitic boulders in the Farol de São Tomé beach (22º02'S, 41º03'W), Rio de Janeiro State. Four sampling profiles using 20 x 20 cm quadrats through a photo quadrat technique revealed four horizontal bands: (1) Littoral Fringe characterized by empty space and Littorina; (2) Upper eulittoral mainly occupied by Chthamalus and bare rock; (3) Lower eulittoral characterized by Chthamalus, Phragmatopoma, Ulva and Centroceras; and (4) Sublittoral Fringe characterized by Ulva, Phragmatopoma and Hypnea. The high variability in the relative abundance of the dominant species among the profiles may be attributed to the high topographic irregularity, reflecting in different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intertidal zonation; Rocky shore; Benthic community; North coast of Rio de Janeiro.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300016

Effect of temperature shock on soybean microspore embryogenesis
Provedor de dados: 52
Autores: Moraes,Ana Paula de; Bonadese-Zanettini,Maria Helena; Callegari-Jacques,Sídia Maria; Kaltchuk-Santos,Eliane.
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anther culture; Soybean; Androgenesis; Symmetrical mitosis; Temperature shock; Stress.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400006

Genetic variation and interrelationships of agronomic characteristics in durum wheat under two constructing water regimes
Provedor de dados: 52
Autores: Talebi,Reza; Fayyaz,Farzad; Naji,Amir Mohammad.
This work aimed to study the grain yield components and plant characteristics related to grain yield. Twenty-four durum wheat genotypes from the ICARDA durum wheat breeding program were grown during 2006-2007 under rainfed and irrigated conditions using a complete randomized block design with three replicate in west of Iran. Correlation and path analysis were carried out. Results showed that there was strong positive association of grain yield with the number of seed/spike, biomass and harvest index. Grain yield was negatively associated with spike length and plant height in different moisture conditions. Comparatively, high genetic variation was found in grain yield and other characteristics. Hierarchical cluster analysis was used as a tool to classify...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Durum wheat; Drought stress; Yield components; Path analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400006

Coffee Berry Borer Resistance in Coffee Genotypes
Provedor de dados: 52
Autores: Sera,Gustavo Hiroshi; Sera,Tumoru; Ito,Dhalton Shiguer; Ribeiro Filho,Claudionor; Villacorta,Amador; Kanayama,Fabio Seidi; Alegre,Clayton Ribeiro; Del Grossi,Leandro.
The aim of this study was to evaluate the coffee germplasm of the Paraná Agronomic Institute (IAPAR) for resistance to the coffee-berry-borer. Preliminary field evaluation was performed in August 2004 and the fruits of less damaged genotypes in the field were evaluated under controlled condition with obligated and free choice experiments established in a randomized complete design with three replications. The genotypes were evaluated fifteen days after infestation with one borer per fruit in Petri dishes. The data were analyzed by the Scott-Knott means test at 1 % and by the χ2 test. Statistical analysis indicated that Coffea kapakata, Psilanthus bengalensis, C. eugenioides and genotypes with C. eugenioides genes were resistant. These genotypes presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Coffea; Cultivars; Hypothenemus hampei; Psilanthus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200003

Estudo comparativo do crescimento de plantas de tomate e milho sob condições de salinidade
Provedor de dados: 52
Autores: Bezerra Neto,Egídio; Nogueira,Rejane Jurema Mansur Custódio.
Plantas de tomate (Lycopersicum esculentum Mill) e de milho (Zea maiz L.) foram cultivadas em meio hidropônico, em presença de 0 ou 100 mmol de NaCl por litro de solução nutritiva. Trinta dias após o início da aplicação dos tratamentos foi realizada coleta para a análise dos parâmetros de crescimento. A taxa de crescimento absoluto de ambas as espécies diminuiu com a salinidade. O tratamento salino não afetou a alocação de biomassa das folhas, provocou uma diminuição na alocação da biomassa do caule e aumento da alocação da biomassa das raízes das plantas de tomate e de milho. Ocorreu uma redução de 48,01 % na área foliar das plantas de milho e de 58,75 % nas de tomate, devido ao tratamento salino. A razão de área foliar nas plantas de milho foi reduzida...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Lycopersicum esculentum; Leaf area; Dry matter; Fresh matter.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400013

Erratum: Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100902

Propriedades funcionais de concentrado protéico extrusado de sementes de girassol
Provedor de dados: 52
Autores: Murate,Elenice H.; Prudencio-Ferreira,Sandra H.
Propriedades funcionais de concentrado protéico de sementes de girassol extrusado a 135, 143 e 151ºC e com 20 e 24% de umidade (base seca) foram avaliadas. Os resultados demonstraram que a extrusão, dependendo das condições, provocou um aumento nos valores de solubilidade da proteína em água, capacidade de absorção de água e gordura, capacidade e atividade emulsificante, estabilidade da emulsão, capacidade de formação de espuma, porém não alterou a propriedade de estabilidade de espuma do concentrado protéico. A solubilidade das proteínas do concentrado protéico extrusado e não extrusado em função do pH foi menor no intervalo de 4,0 a 6,0 e houve um aumento significativo a partir do pH 7,0 para a amostra não extrusada.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteína; Girassol; Extrusão; Propriedades funcionais.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200012

Extraction of pectin from apple pomace
Provedor de dados: 52
Autores: Canteri-Schemin,Maria Helene; Fertonani,Heloísa Cristina Ramos; Waszczynskyj,Nina; Wosiacki,Gilvan.
As apple-processing units are now in expansion in Brazil, industrial by-products like pomace play an important role in pectin manufacture. The objective of this article was to determine a pratical follow-up to the extraction of pectin from apple pomace and to characterize it in a laboratory, on a small scale, aiming at establishing the optimum conditions for acid extraction. The highest yields were obtained when [1]apple pomace was dried and ground to obtain an apple flour to be used as raw material, [2] citric or nitric acids were used and [3] when the citric acid concentration was 6.2 g/100 ml and the time of reaction was 153 minutes. The apple variety in itself was not significant in pectin yield. The degree of esterification (DE = 68.84 %) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple pomace; Pectin; Agricultural wastes; Yield; Extraction; Response Surface Methodology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200013

Oxidative Damage and Antioxidant Response in Chenopodium murale L. Exposed to Elevated Levels of Zn
Provedor de dados: 52
Autores: Zoufan,Parzhak; Karimiafshar,Amer; Shokati,Sara; Hassibi,Payman; Rastegarzadeh,Saadat.
ABSTRACT Present study was carried out to investigate the effects of different Zn concentrations on growth and some antioxidant responses in Chenopodium murale L. The seeds were sown on the pots. 45-day plants were transferred to hydroponic solution containing 0.2 (control), 150, 300 and 600 µM Zn. The plants were harvested at 6 days after applying Zn treatments and some parameters were evaluated including plant length, fresh weight, photosynthetic pigments, malondialdehyde (MDA), ascorbate, proline and enzymatic antioxidants such as catalase (CAT), guaiacol peroxidase (GPX) and superoxide dismutase (SOD). Zn concentration showed a considerable increase in the shoot and root as the concentration of Zn increased in the medium, meanwhile the roots were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbate; Enzymatic antioxidant; Lipid peroxidation; Proline; Zinc.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100418

Effect of different levels of sodium chloride and glucose on fermentation of sardines (Sardinella brasiliensis) by Lactobacillus sakei 2a
Provedor de dados: 52
Autores: Espirito Santo,Milton Luiz Pinho; Lisboa,Cristiane; Alves,Fernanda Gonçalves; Martins,Daniela; Beirão,Luiz Henrique; Sant'Anna,Ernani Sebastião; Franco,Bernadette Dora Gombossy de Melo.
Lactobacillus sakei 2a is a bacteriocin producer strain. In this study, it's effects as a starter culture in the curing process of sardine (Sardinella brasiliensis) fillets were studied at different concentrations of NaCl (2, 4, 6%) and glucose (2, 4%). After 21 days of fermentation, the spoilage microorganisms population reached 9.7 Log10 CFU g-1 corresponding to 6% NaCl and 4% glucose. With no addition of glucose and starter culture, sardine fillets began spoilage 72 hours after fermentation, even when 6% NaCl was used. Little differences were observed in lactic acid production when 2 and 4% glucose were added, since total acidity was 1.32 and 1.34% respectively, the experiments with 6% NaCl presented the best results. Initial pH of sardine fillets was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sardinella brasiliensis; Lactobacillus sakei 2a; Fermented fish.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100008

Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Carobinha-do-campo)
Provedor de dados: 52
Autores: Carrim,Aysha Jussara Ivonilde; Barbosa,Edweis Cândida; Vieira,José Daniel Gonçalves.
The objectives of this work was to isolate endophytic bacteria from Jacaranda decurrens Cham. and screening of some enzymes of biotechnological interest. Ten (10) bacterial species were isolated and identified from the leaves and steams. All the isolates presented enzymatic activity, which was ranked as follows: proteolytic (60%) and amilolytic activity (60%), lipolytic (40%), esterasic (40%). However, cellulolytic and pectinolytic activities were not detected. This is the first report on the isolation and identification of endophytic bacteria from Jacaranda decurrens Cham.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic bacteria; Jacaranda decurrens; Enzymes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400001

Biochemical and morphological aspects of salivary glands of the predator Brontocoris tabidus (Heteroptera: Pentatomidae)
Provedor de dados: 52
Autores: Azevedo,Dihego de Oliveira; Zanuncio,José Cola; Zanuncio Jr.,José Salazar; Martins,Gustavo Ferreira; Marques-Silva,Solange; Sossai,Marcos Franklin; Serrão,José Eduardo.
The aim of this work was to study the feeding habits of the predator Brontocoris tabidus (Heteroptera: Pentatomidae) analyzing the salivary gland complex (SGC). The SGC was dissected and subjected to histological analyses and biochemical assays. Results showed that a pair of bilobed principal salivary glands and one pair of tubular accessory salivary glands form the SGC and different parts of salivary gland synthesizes similar substances. Lipases, a-amylase and trypsin-like enzymes were detected at both lobes of the principal salivary gland. These data together with observations related to the predator's diet suggested that it could be considered an obligate zoophytophagous.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salivary glands; Biological control; Heteroptera; Pentatomidae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300013

Prevalence of estrogen receptor alpha PvuII (c454-397T&gt;C) and XbaI (c454A&gt;G) polymorphisms in a population of Brazilian women
Provedor de dados: 52
Autores: Araújo,Karine Lourenzone; Rezende,Lucas Cunha Dias de; Souza,Letícia Soncini; Daltoé,Renata Dalmaschio; Madeira,Klesia Pirola; Paes,Marcela Ferreira; Herkenhoff,Fernando Luiz; Rangel,Letícia Batista Azevedo; Silva,Ian Victor.
The aim of this work was to study the estrogen receptor alpha (ERS1) PvuII and XbaI gene polymorphisms prevalence in randomly selected women population from Rio de Janeiro and Espírito Santo states in Brazil by polymerase chain reaction restriction fragment lengths polymorphism (PCR_RFLP) methodology. It was shown that Rio de Janeiro women exhibited a significantly different prevalence of XbaI polymorphism comparing to Espirito Santo women. Nonetheless, similar prevalence of PvuII polymorphism was found in both women´s populations. Moreover, a strong linkage disequilibrium was observed between these SNPs reinforcing the hypothesis of differential pattern of inheritance observed on such populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Estrogen Receptor; Brazilian Women; Prevalence; Linkage disequilibrium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600010

Influence of high osmotic pressure on sorbitol production by Zymomonas mobilis
Provedor de dados: 52
Autores: Vignoli,Josiane Alessandra; Cazetta,Marcia Luciana; Silva,Rui Sérgio Santos; Celligoi,Maria Antonia Pedrine Colabone.
The objective of the present work was to study the variation on the sorbitol production in relation to the concentration of sugars, (metabolizable or not) and the cultivation time. A full factorial design was used considering the factors such as sucrose and maltose concentration and cultivation time. The addition of sugars caused increases on the sorbitol production up to the concentration of 300g/L however, decreases on the sorbitol production were observed when the concentration reached values above this. Increasing the time of fermentation was statistically significant to sorbitol production, however, little increase the production was noticed after 36h.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymomonas mobilis; Sorbitol; High osmotic stress; Maltose.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500022

Behavioral and ecological interactions between reintroduced golden lion tamarins (Leontopithecus rosalia Linnaeus, 1766) and introduced marmosets (Callithrix spp, Linnaeus, 1758) in Brazil's Atlantic Coast forest fragments
Provedor de dados: 52
Autores: Ruiz-Miranda,Carlos Ramon; Affonso,Adriana Gomes; Morais,Marcio Marcelo de; Verona,Carlos Eduardo; Martins,Andreia; Beck,Benjamin B.
Marmosets (Callithrix spp.) have been introduced widely in areas within Rio de Janeiro state assigned for the reintroduction of the endangered golden lion tamarin (Leontopithecus rosalia). The objetives of this study were to estimate the marmoset (CM) population in two fragments with reintroduced golden lion tamarin to quantify the association and characterize the interactions between species. The CM population density (0,09 ind/ha) was higher than that of the golden lion tamarin (0,06 ind/ha). The mean association index between tamarins and marmosets varied among groups and seasons (winter=62% and summer=35%). During the winter, competition resulted in increases in territorial and foraging behavior when associated with marmosets. Evidence of benefits...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecology; Behavior; Golden lion tamarin; Common marmoset; Invasive species; Conservation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100012

Microencapsulation of a Colombian Spodoptera frugiperdaNucleopolyhedrovirus with Eudragit® S100 by spray drying
Provedor de dados: 52
Autores: Camacho Kurmen,Judith Elena; Gómez Alvarez,Martha Isabel; Villamizar Rivero,Laura Fernanda.
A Colombian Spodoptera frugiperda nucleopolyhedrovirus NPV003 with high potential for the development of an efficient biopesticide was microencapsulated by spray drying with a pH dependent polymer (Eudragit® S100). Conditions for microparticles production were standardized and microencapsulation process was validated. Physical properties, insecticide activity and photo-stability of microencapsulated virus were determined. The microparticles were spherical and irregular shaped, with sizes between 17.64 and 19.47 µm. Moisture content was 10.38 ± 0.87%; encapsulation efficiency 84.61± 13.09% and process yield was 91.20 ± 6.40%. Microencapsulation process did not affect viral insecticidal activity and provided efficient protection against UVB radiation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; Microencapsulation; Spray drying; Eudragit® S100; Photostability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300468

Role of Light and Dark Cycle and Different Temperatures in the Regulation of Growth and Protein Expression in Oscillatoria agardhii Strain
Provedor de dados: 52
Autores: Dautania,Gajendra Kumar; Singh,Gajendra Pal.
The cyanobacterium Oscillatoria agardhii was isolated from the fresh water Mawatha lake, Jaipur and was grown in Zarrouk's medium at 25 ± 2°C, illuminated with white fluorescent light at the intensity of 2 500 lux with 12:12 h light and dark photoperiod. The effects of photoperiod and temperature on the growth and protein expression by Oscillatoria agardhii were studied under different controlled culture conditions (ALR, ALC, CLR, CLC, and NDL), measuring optical density, cell count and dry weight. Protein content was measured quantitatively by Bradford assay and qualitatively by SDS-PAGE. The densitometric analysis was also carried out for the measurement of the expression level of different proteins/peptides under different culture conditions. Maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradford assay; Densitometer; Oscillatoria agardhii; Photoperiod; SDS-PAGE.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600933

Formulated products containing a new phytase from Schyzophyllum sp. phytase for application in feed and food processing
Provedor de dados: 52
Autores: Salmon,Denise Naomi; Piva,Luíza Cesca; Binati,Renato Leal; Vandenberghe,Luciana Porto de Souza; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Spier,Michele Rigon.
A new formulated product containing high yield of phytase from Schizophyllum sp., an important mushroom used for medicinal studies, was developed for application in feed industries and for future use in food processing. The enzyme presented a high activity yield 55.5 U/mL and 6240 U/gds in liquid and solid formulated product, respectively. It showed a good shelf-life in concentrated product, retaining 67.8% of its activity after 60 days of storage at room temperature and 90% of the activity was maintained in the liquid formulation after the same period. Powder bioformulated product maintained 77% of its activity after two months of storage, without the addition of chemical additives, which was named as a new bioformulated product containing high quantities...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytase; Shizophyllum sp; Downstream steps; Stability; Formulation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600001

Genetic variability of brycon orbignyanus (valenciennes, 1850) (characiformes: characidae) in cultivated and natural populations of the upper paraná river, and implications for the conservation of the species
Provedor de dados: 52
Autores: Panarari-Antunes,Renata de Souza; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Galdino,Alexsandro Sobreira; Julio Junior,Horácio Ferreira; Prioli,Laudenir Maria.
This study aimed to evaluate the genetic variability of B. orbignyanus in cultivated and natural populations of the Upper Paraná River, using molecular RAPD markers and mtDNA control region. Specimens were collected in the Paraná River and in the Piracema fish farm in Maringá, State of Paraná, Brazil. RAPD primers produced 82 loci with consistent expression. The population from the Paraná River showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. Data revealed genetic differentiation between the two populations, although not very pronounced. These results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. The alignment of the D-loop sequences of B. orbignyanus indicated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Endangered; RAPD; MtDNA; Polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400025

Improved purification process of β- and α-trypsin isoforms by ion-exchange chromatography
Provedor de dados: 52
Autores: Santos,Alexandre Martins Costa; Oliveira,Jamil Silvano de; Bittar,Eustáquio Resende; Silva,Anderson Lourenço da; Guia,Marcos Luiz dos Mares; Bemquerer,Marcelo Porto; Santoro,Marcelo Matos.
The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4ºC. The sample loading, salt concentration, flow rate and pH of mobile phase were varied to determine their effects on the resolution of the separation. The resolution was optimized mainly between β- and α-trypsin. Pure isoforms were obtained by chromatographying 100 mg of commercial trypsin during seven days, yielding 51 mg of high purity β-trypsin and 13 mg of α-trypsin partially pure, with small amounts of contaminating of ψ-trypsin....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypsin isoforms; Resolution; Ion-exchange chromatography; Purification; Specific activity; Mass spectrometry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009

The genus Enterococcus as probiotic: safety concerns
Provedor de dados: 52
Autores: Araújo,Tatiane Ferreira; Ferreira,Célia Lúcia de Luces Fortes.
Species from the genus Enterococcus have been used as probiotic for humans or animals, although this genus is not considered "generally recognized as safe" (GRAS). While enterococci are considered "positive" in food technology, isolates of this genus have emerged as opportunistic pathogens for the humans. The aim of this review is to summarize the characteristics that can determine the use of this genus as probiotics. According to the guidelines used to define the genus Enterococcus strains as probiotic a case-by-case evaluation of each potential technological strain is presented and research perspectives for using enterococci as probiotic is also discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disease; Enterococcus; Probiotic; Safety aspect.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300014

Infection imaging with radiopharmaceuticals in the 21st century
Provedor de dados: 52
Autores: Das,Satya S.; Hall,Anne V.; Wareham,David W.; Britton,Keith E.
Infection continues to be a major cause of morbidity and mortality worldwide. Nuclear medicine has an important role in aiding the diagnosis of particularly deep-seated infections such as abscesses, osteomyelitis, septic arthritis, endocarditis, and infections of prosthetic devices. Established techniques such as radiolabelled leucocytes are sensitive and specific for inflammation but do not distinguish between infective and non-infective inflammation. The challenge for Nuclear medicine in infection imaging in the 21st century is to build on the recent trend towards the development of more infection specific radiopharmaceuticals, such as radiolabelled anti-infectives (e.g. 99mTc- ciprofloxacin). In addition to aiding early diagnosis of infection, through...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Imaging; Infection; Inflammation; Radiolabelled anti-infectives; 99mTc- ciprofloxacin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500005

Radionuclide imaging of infection: what the future holds
Provedor de dados: 52
Autores: Palestro,Christopher J.
Nuclear Medicine plays an important role in the evaluation of patients suspected of harboring infection. Gallium imaging is especially useful for opportunistic infections and spinal osteomyelitis. In vitro labeled leukocyte imaging is the current radionuclide gold standard for imaging most infections, in immunocompetent patients, including cardiovascular, postoperative, and musculoskeletal infections (except spinal osteomyelitis). Several in-vivo leukocyte labeling methods have been investigated, but none are widely used. Results obtained with radiolabeled antibiotics have been disappointing. Data on FDG are still emerging, but this agent appears to be especially valuable in fever of unknown origin, spinal osteomyelitis, vasculitis and sarcoidosis. It is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infection; Labeled leukocytes; Gallium; FDG; PET.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700002

Effect of inoculation associated to leaf sprayed Co+Mo on the yield and grain nutrients in common bean (Phaseolus vulgaris L)
Provedor de dados: 52
Autores: Berton Junior,João Francisco; Santos,Julio Cesar Pires; Coelho,Cileide Maria Medeiros; Klauberg Filho,Osmar.
The objective of this work was to evaluate the efficiency of nitrogen fixing inoculum associated with Co + Mo leaf spray on the common bean grain yield and grain nutrients, cv. FT Nobre. Three dosages of the inoculant (0, 200 and 400 g/50 kg seeds), combined with four Co + Mo leaf spray levels (T0=0,0; T1=4.9,49; T2=7.3,73; and T3=9.7,97 g ha-1 of Co and Mo, respectively) were tested. The grain yield with the use of the inoculant (400 g / 5O kg seed-1) associated with the higher level of Co+Mo (T2 and T3) was very similar to the mineral nitrogen condition fertilizer recommended for the bean (70 kg ha-1 of N). With the increased inoculant dosage, an increase of the protein content and of P and Mg in the grain was also observed. The results indicated that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seed quality; Phosphorus; Magnesium; Nitrogen.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600002

The Mutagenic Potential Caused by the Emissions from Combustion of Crude Glycerin and Diesel Fuel
Provedor de dados: 52
Autores: Mazak,Daniel Terruggi; Ávila,Ivonete; Crnkovic,Paula Manoel; Cordoba,Aymer Yeferson Maturana; Pagliuso,Josmar Davilson.
This study evaluated the use of crude glycerin as an alternative of energy generation to replace the traditional fuels. The Tradescantia stamen hair mutation assay (Trad-SH) was applied to study the mutagenic effects caused by the emissions generated in the direct combustion of diesel oil and glycerin in a flame tube furnace. Tradescantia inflorescences were exposed to gaseous emissions from the combustion tests in a fumigation chamber for 30-40 min. The analysis of variance and the Tukey test were applied to compare the differences between six test groups (intoxicated with emissions from glycerin and diesel oil combustion) and a control group. Only one glycerin group showed statistical differences (0.05), possibly due to the complexity of the burning...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tradescantia; Stamen-hair assay; Toxic emissions; Glycerin; Diesel.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200309

Erratum: Evaluation of the Blue Crab Callinectes sapidus Rathbun, 1896 Bycatch in Artisanal Fisheries in Southern Brazil
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100901

Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro
Provedor de dados: 52
Autores: Souza,Fernanda Vidigal Duarte; Garcia-Sogo,Begoña; Souza,Antonio da Silva; San-Juán,Amparo Pérez; Moreno,Vicente.
Callus cultures from cotyledon and leaf explants of a Spanish cultivar of melon (Amarillo Oro) were tested for growth and morphogenic capacity on several culture media with different concentrations of IAA (indole-3-acetic acid) in combination with 1.0 mg.L-1 BA (6-benzylaminopurine) or 6.0 mg.L-1 KIN (kinetin). The best results were achieved with cotyledon explants. The leaf explants presented low bud formation capacity. Variability of organogenic response on cotyledons of different age (7, 5, 3 and 1-day-old) was evaluated. The age of explant had a significant influence on bud induction. Cotyledon explants from 7-day-old seedlings showed higher organogenic index and development of shoots when cultured onto MS medium supplemented with 1.5 mg.L-1 of IAA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organogenesis; Plant regeneration; Leaf explant; Cotyledon explant; Cucurbitaceae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100003

The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
Provedor de dados: 52
Autores: Hasenack,Beatriz S.; Botelho,Maria Valéria J.; Lauretti,Flávio; Melo,Fernando L. de; Orlandi,Janaina M.; Linhares,Rosa Elisa C.; Ueda,Marli; Nozawa,Carlos M.
Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Concanavalin A; Cell culture.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003

Reproduction and longevity of Supputius cincticeps (Het.: Pentatomidae) fed with larvae of Zophobas confusa, Tenebrio molitor (Col.: Tenebrionidae) or Musca domestica (Dip.: Muscidae)
Provedor de dados: 52
Autores: Zanuncio,José Cola; Beserra,Eduardo Barbosa; Molina-Rugama,Adrián José; Zanuncio,Teresinha Vinha; Pinon,Tobias Baruc Moreira; Maffia,Vanessa Pataro.
Reproduction and longevity of Supputius cincticeps (Stål) (Heteroptera: Pentatomidae) fed on Zophobas confusa Gebien, Tenebrio molitor L. (Coleoptera: Tenebrionidae) or Musca domestica (L.) (Diptera: Muscidae) larvae were studied during two generations at 24.7 ± 1.1ºC, 70 ± 10% R.H. and 12 h of photophase. Body weight of newly-emerged adults, oviposition period, number of egg masses, total number of eggs and longevity of S. cincticeps were higher when fed on Z. confusa or T. molitor larvae than on M. domestica larvae. Regardless of diet, S. cincticeps showed better reproduction and longevity in the second generation in laboratory conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asopinae; Predatory stinkbug; Fecundity; Alternative prey.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600013

Current evaluation of the clinical utility of Fluoromethylcholine-(18F) PET/CT in Prostate Cancer
Provedor de dados: 52
Autores: Talbot,Jean-Nöel; Chevalme,Yanna-Marina.
This short review is dedicated to the current status of the assessment of a new PET radiopharmaceutical, fluoromethylcholine-(18F) or FCH, which is taken-up by prostate cancer tissue, in contrary to fluorodeoxyglucose-(18F) or FDG. It seems that FCH could become "the FDG of prostate cancer", with the same type of achievements (detection of distant metastases and of occult recurrences, restaging prior to invasive treatments), and the same drawbacks (false negative results in case of small lesions, in particular lymph nodes metastases, and false positive results in case of infection/inflammation, in particular prostatitis). Current evidence is summarised and discussed for each of the potential settings of FCH PET/CT imaging in prostate cancer. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoromethylcholine-(18F); PET/CT; Prostate cancer; Evaluation of radiopharmaceuticals.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700012

Physiological quality in seeds obtained by topcrosses between vegetable soybean and grain type
Provedor de dados: 52
Autores: Yokomizo,Gilberto K.; Vello,Natal A.
Attempts were made to study the gene introgression for high physiological quality from adapted cultivars in the vegetable soybean (exotic). Promising results were obtained for all topocrosses, with increased in germination percentage in majority. General mean of the germination percentage in topcrosses with large seeds exotic parental showed an increase from 28 to 46%, and topcrosses with small seeds exotic parental, from 54 up to 60%, both cases compared to self pollinated exotic parental. Related to variance, the topcrosses showed higher values than the self pollinated exotic parental, showing to be possible the selecion of promising materials.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Glycine max; Topcross; Physiological quality in seeds; Vegetable soybean.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500004

Profile of milk fatty acids from moxotó goats fed with different levels of manicoba (Manihot Glaziovii Muel Arg.) silage
Provedor de dados: 52
Autores: Mesquita,Ítala Viviane Ubaldo; Costa,Roberto Germano; Queiroga,Rita de Cássia Ramos do Egypto; Medeiros,Ariosvaldo Nunes de; Schuler,Alexandre Ricardo Pereira.
The objective of this work was to evaluate the effects of the manicoba silage use (Manihot glaziovii Muel Arg.) in different roughage:concentrate ratios (30:70; 40:60; 50:50 and 60:40 %) on the fatty acids profile of the milk from Moxotó goats. Eight multiparous goats with approximately 60 post-birth days and weigh 44 kg on average were used in a Double Latin Square with four treatments, four periods and four animals. Each period lasted 15 days with 10 days of adaptation to experimental diets and 5 days of milk collection. The results of the fatty acids analyses were submitted to the analysis of variance (ANOVA) and regression. An increasing linear effect for the miristic acid (C14:0) and decreasing for the linoleic acid (C18:3) was observed in function of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nourishment; Lipids; Milk quality; Native breed.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600011

Blackberry Vinegar Produced By Successive Acetification Cycles: Production, Characterization And Bioactivity Parameters
Provedor de dados: 52
Autores: Cunha,Mário Antônio Alves da; Lima,Kely Priscila de; Santos,Vidiany Aparecida Queiroz; Heinz,Otto Lucas; Schmidt,Carla Adriana Pizarro.
ABSTRACT: Blackberry vinegar was produced in successive acetification cycles and content of total phenolics, anthocyanins and antioxidant activity were evaluated along the production. Firstly, blackberry wine was obtained in bench-scale bioreactor, being verified 0.39 g/g ethanol yield, 1.78 g/L.h volumetric productivity and 76% efficiency. After, three successive acetification cycles were conducted efficiently in grapia barrel with average acetic acid production of 51.6 g/L, 72.2 % acetic acid yield and 0.4 g/L.h volumetric productivity. Appreciable contents of polyphenolic compounds, anthocyanins and high antioxidant activity were observed in the raw material, wine and vinegar obtained in each cycle of acetic acid transformation. Acetic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Berries; Acetic Acid Bacteria; Bioactives; Wine; Fermentation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100508

Limnological study of Piraquara river (Upper Iguaçu basin): spatiotemporal variation of physical and chemical variables and watershed zoning
Provedor de dados: 52
Autores: Marques,Paulo Henrique C.; Oliveira,Haydée Torres de; Machado,Eunice da Costa.
The Piraquara river basin (Upper Iguaçu River basin - Brazil) was studied as an ecological system throughout a complete seasonal cycle, comprising the rainy and dry season. Analyzes of 16 physical and chemical water variables (dissolved oxygen, biochemical oxygen demand, temperature, pH, conductivity, total nitrogen, total phosphorus, ortophosphates, nitrite, nitrate, ammonium, reagent silicate, total suspended solids, chlorophyll - a, flow velocity and depth) showed correlations between water composition and watershed physiographic features, and the Principal Component Analysis allowed to evidence spatial gradients and seasonal differences. The sampling points were clustered in patches with homogeneous behavior, according to ecologycal concepts: patch 1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lotic Ecossystems; Water Chemistry; Subtropical Stream; River Continuum; Upper Iguaçu Watershed.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300010

Extension of the IsaViz software for the representation of metabolic and regulatory networks
Provedor de dados: 52
Autores: Veiga,Diogo Fernando; Cecconello,Pedro de Stege; Lucca,José Eduardo de; Porto,Luismar Marques.
In this work we developed an extension of IsaViz software, a RDF (Resource Description Framework) authoring tool, designed to be a graphical environment to build models of metabolic and regulatory networks. This environment, called Metabolic IsaViz, was linked to a genomic library of types and was modeled on the basis of ontologies. Biochemical pathways included data at sequence level (e.g., the amino acid sequence of enzymes), besides kinetic and thermodynamic parameters for the reactions. Models created with Metabolic IsaViz could be exported to pathways simulators through SBML (Systems Biology Markup Language), which allowed to analyze the pathway dynamics of target chemicals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic and Regulatory Pathways; RDF/XML; Modeling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400025

Antioxidant activity of the extracts from Pycnoporus sanguineus mycelium
Provedor de dados: 52
Autores: Borderes,Josiane; Costa,Alessandra; Guedes,Alessandro; Tavares,Lorena Benathar Ballod.
The aim of this work was to study the production of compounds with antioxidant activity by Pycnoporus sanguineus when cultivated in submerged fermentation using a potato dextrose broth plus peptone medium. The study evaluated the biomass production, glucose consumption, variation of medium pH and antioxidant activity. The antioxidant potential was tested through the DPPH method and β-carotene / linoleic acid system with extracts obtained from the mycelium at different times of cultivation (5, 10, 15, 20, 25 and 30 days). Maximum kinetic values of specific growth rate (0.289 day-1), biomass productivity (0.698 g.L-1.day-1) and the yield of glucose conversion into biomass (26.24 g.g-1) were obtained during the exponential growth phase. The highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basidiomycetes; Submerged culture; Kinetics; Antioxidant compounds.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600012

Microbial succession within an anaerobic sequencing batch biofilm reactor (ASBBR) treating cane vinasse at 55ºC
Provedor de dados: 52
Autores: Ribas,Maria Magdalena Ferreira; Chinalia,Fabio Alexandre; Pozzi,Eloisa; Foresti,Eugenio.
The aim of this work was to investigate the anaerobic biomass formation capable of treating vinasse from the production of sugar cane alcohol, which was evolved within an anaerobic sequencing batch biofilm reactor (ASBBR) as immobilized biomass on cubes of polyurethane foam at the temperature of 55ºC. The reactor was inoculated with mesophilic granular sludge originally treating poultry slaughterhouse wastewater. The evolution of the biofilm in the polyurethane foam matrices was assessed during seven experimental phases which were thus characterized by the changes in the organic matter concentrations as COD (1.0 to 20.0 g/L). Biomass characterization proceeded with the examination of sludge samples under optical and scanning electron microscopy. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaerobic digestion; Adaptation phase; Wastewater; Sugar cane; Biofilm; Microbiology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400028

Isolation and screening of microorganisms with potential for biotransformation of terpenic substrates
Provedor de dados: 52
Autores: Bier,Mário César Jucoski; Poletto,Sabrina; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Medeiros,Adriane Bianchi Pedroni.
The objective of the present work was to isolate and select strains with potential to perform the biotransformation of terpenic substrates. Microorganisms obtained from a collection culture and also isolated from a natural source of terpene substrate were tested. Seventeen strains were selected by their resistance to terpenes in potato dextrose agar containing up to 1% of limonene or α-pinene and β-pinene (1:1). Subsequently, 10 strains were selected by their capacity of using these terpenes as sole carbon source in a mineral medium. The biotransformation capacity of these strains was tested and the products obtained were identified by GC-MS.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aroma; Flavour; Limonene; Β-pinene; Biotransformation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500020

Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells
Provedor de dados: 52
Autores: Neves,Rosane de Figueiredo; Moreno,Silvana Ramos Farias; Rebello,Bernardo Machado; Caldas,Luiz Querino de Araújo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arctium lappa; Technetium-99m; Blood constituents; Morphometry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600021

Exposure of pampas fox (Pseudalopex gymnocercus) and crab-eating fox (Cerdocyon thous) from the Southern region of Brazil to Canine distemper virus (CDV), Canine parvovirus (CPV) and Canine coronavirus (CCoV)
Provedor de dados: 52
Autores: Hübner,Silvia de Oliveira; Pappen,Felipe Geraldes; Ruas,Jerônimo Lopes; Vargas,Gilberto D'Ávila; Fischer,Geferson; Vidor,Telmo.
The exposure of 13 Brazilian free-ranging nondomestic canids (five pampas fox - Pseudalopex gymnocercus and eight crab-eating fox -Cerdocyon thous) from Southern region of Brazil, to Canine distemper virus (CDV), canine parvovirus (CPV) and Canine coronavirus (CCoV) was investigated. Antibodies against CDV were detected in 38.5% (5/13) of the samples. There were anti-CDV antibodies in 60% (3/5) of P. gymnocercus and in 25% (2/8) of C. thous. The frequency was higher among the adults and males. Eleven canids (84.6%) presented antibodies against CPV, 80% (4/5) were from P. gymnocercus and 87.5% (7/8) were from C. thous. There was no difference in positivity rate against CPV between gender and age. Antibodies against CCoV were detected in 38.5% (5/13) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudalopex gymnocercus; Cerdocyon thous; Canine distemper virus (CDV); Canine parvovirus (CPV); Canine coronavirus (CCoV); Serology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300012

Effect of Phosphorylation and Copper(II) or Iron(II) Ions Enrichment on Some Physicochemical Properties of Spelt Starch
Provedor de dados: 52
Autores: Rożnowski,Jacek; Fortuna,Teresa; Nowak,Katarzyna; Szuba,Edyta.
ABSTRACT: This paper provides an assessment of the effect of saturation of spelt starch and monostarch phosphate with copper or iron ions on selected physicochemical properties of the resulting modified starches. Native and modified spelt starch samples were analyzed for selected mineral element content using Atomic Absorption Spectroscopy (AAS). Thermodynamic properties were measured using DSC, and pasting properties by RVA. Flow curves of 5% pastes were plotted and described using the Herschel-Bulkley model. The structure recovery ratio was measured. AAS analysis established the presence of iron(II) and copper(II) ions in the samples of modified starches and that potassium and magnesium ions had leached from them. In comparison to unfortified samples,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemically modified starch; Enrichment; Phosphorylation; Spelt starch.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100511

In Vitro antileishmanial properties of neutron-irradiated meglumine antimoniate
Provedor de dados: 52
Autores: Borborema,Samanta Etel Treiger; Andrade Junior,Heitor Franco de; Osso Junior,João Alberto; Nascimento,Nanci do.
Pentavalent antimony, as meglumine antimoniate (Glucantime® ) or sodium stibogluconate (Pentostam® ), is the main treatment for leishmaniasis, a complex of diseases caused by the protozoan Leishmania, and an endemic and neglected threat in Brazil. Despite over half a century of clinical use, their mechanism of action, toxicity and pharmacokinetic data remain unknown. The analytical methods for determination of antimony in biological systems remain complex and have low sensitivity. Radiotracer studies have a potential in pharmaceutical development. The aim of this study was to obtain a radiotracer for antimony, with suitable physical and biological properties. Meglumine antimoniate was neutron irradiated inside the IEA-R1 nuclear reactor, producing two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meglumine antimoniate; Leishmaniasis; Leishmania (L.) chagasi; Radiotracer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700009

Erratum: Economic Viability of Tomato Cultivation in Organic Farming System
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100918

Evaluation of trace metals (cadmium, chromium, copper and zinc) in tissues of a commercially important fish (Leporinus obtusidens) from Guaíba Lake, Southern Brazil
Provedor de dados: 52
Autores: Costa,Silene de Carvalho; Hartz,Sandra Maria.
Concentrations of Cd, Cr, Cu and Zn were determined in liver and muscle of Leporinus obtusidens. During 2003, the fishes (N = 54) were sampled from three sites along Guaíba Lake (north, central and south zones). The hypotheses tested assumed that there were differences in the location and date as to levels of contamination, association between the trace metal content in the fishes and size, and that the sex influenced metal accumulation. Possible risks of contaminated fish consumption to human health were also evaluated. The level of mean concentration (µg/g) of metals in tissues was higher in liver than muscle: [Cd] = 2.32♂; [Cr] = 0.08♀; [Cu] = 30.82♀ and [Zn] = 97.77♀. The results showed differences between the local and date sampling for all metals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trace metals; Leporinus obtusidens; Environmental contamination; Guaíba Lake; Southern Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100029

Comparison of different methodologies for DNA extraction from Aegla longirostri
Provedor de dados: 52
Autores: Bitencourt,João Vitor Trindade; Roratto,Paula Angélica; Bartholomei-Santos,Marlise Ladvocat; Santos,Sandro.
The aim of this study was to compare some DNA extraction methodologies for Aegla longirostri. The protocols were based on the traditional phenol-chloroform DNA extraction methodology and using a commercial kit for DNA extraction. They differed in tissues used, the addition - or not - of beta-mercaptoethanol to the lysis buffer, times and methods for the animal's conservation (frozen, in ethanol or fresh). Individuals stored at -20°C for a long time supplied lower molecular weight DNA than those stored for a short time. The best yield for the specimens preserved in ethanol was obtained for 15 days storage in 95% ethanol. The kit resulted in a low quantity of high molecular weight DNA. The best protocol for DNA extraction from Aeglidae, and probably for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anomura; Aeglidae; Freshwater crab; DNA extraction.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700010

Seafood as functional food
Provedor de dados: 52
Autores: Soccol,Marcilene C. Heidmann; Oetterer,Marília.
Functional food presents specific physiological properties, supplies nutrients and can be naturally found in formulated food or added to it. Seafood plays an important role in human diet, representing the largest stock available of w-3 polyunsaturated fatty acids, especially eicosapentaenoic (EPA) and docosahexaenoic (DHA). Scientific researchers have intensified their studies on fatty acids due to their importance in preventing and/or curing diseases, especially cardiovascular and inflammatory ones. This review describes the most important aspects of w-3 fatty acids found in fish, as well as their roles in the mechanism involved in the prevention and control of diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Seafood; Polyunsaturated fatty acids; W-3 fatty acids; Functional foods.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300016

Genome-Wide Assessment of Putative Superoxide Dismutases in Unicellular and Filamentous Cyanobacteria
Provedor de dados: 52
Autores: Prajapati,Rajesh; Yadav,Shivam; Mitra,Sonali; Rai,Priya; Mishra,Rajeev; Atri,Neelam.
Abstract Cyanobacteria are photoautotrophic prokaryotes capable to grow in diverse ecological habitats, originated 2.5-3.5 billion years ago and were first to produce oxygen. Since then superoxide dismutases (SOD) acquired great significance due to their ability to catalyze detoxification of byproducts of oxygenic photosynthesis i.e. superoxide radicals. In the present study, we extracted information regarding SODs from species of sequenced cyanobacteria and investigated their diversity, conservation, domain structure, and evolution. 144 putative SOD homologs were identified. Unlike other protein families (ex. serine-threonine kinases) SODs are present in all cyanobacterial species reflecting their significant role in survival. However, their distribution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide dismutases; Cyanobacteria; Comparative genomics; Phylogeny.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100422

Evaluation of rat plasma proteins after bothropic venom inoculation and treatment with Mesenquimal stem cells
Provedor de dados: 52
Autores: Telles,Thalita Costa; Michel,Ana Flávia Ribeiro Machado; Lúcia,Maria; Melo,Bruno Martins; Bicalho,Adriane Costa-Val Pimenta; Ocarino,Natália Melo; Melo,Marilia Martins.
The aim of this work was to evaluate the mesenchymal stem cells treatment of rats with myonecrosis caused by Rhinocerophis alternatus venom through acute phase proteins (APP) profile. The animals were distributed into three experimental groups (G1, G2 and G3). G1 and G2 were inoculated with 120 μg of R. alternatus venom diluted in 200 µL of ultra-pure water in gastrocnemic muscle, while G3 received 200 µL of ultra-pure water. Three days after, G1 was treated with 5 X 10(6) MSC diluted in PBS and G2 and G3 only with PBS. Each three days after the treatments (3rd, 6th, 9th, 12th 15th days), blood of five animals in each group was collected in order to evaluate the APP. A decrease (P&lt;0.05) in α2-globulin fraction was observed in G1 on the 6th day. In G1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhinocerophis alternatus venom; Rats; Mesenquimal stem cell; Inflammatory proteins.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100005

Dormancy breaking and germination of Enterolobium contortisiliquum (Vell.) Morong seed
Provedor de dados: 52
Autores: Malavasi,Ubirajara Contro; Malavasi,Marlene de Matos.
Enterolobium contortisiliquum (Vell.) Morong. is a Mimosaceae tropical tree species recommended for heterogeneous reforestation of degraded tropical areas. "Timburi" seeds present low germination due to the high degree of seed dormancy. Different methods to overcome seed dormancy was compared: sanding, sanding followed by 24 hours water (25ºC) soaking, imbibition in boiling water followed by exposure to water at room temperature (28ºC), and concentrated sulfuric acid (5, 15, 30, 60, 120 or 180 minutes) followed by washing with tap water. All seeds were germinated in rolled towels at 25ºC and 12 hours photoperiod. Total germination, first count of germination test and germination velocity index were recorded. Mechanical scarification (sanding), chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dormancy; Forest nursery; Germination; Seed.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600003

Use of fish waste as silage: a review
Provedor de dados: 52
Autores: Arruda,Lia Ferraz de; Borghesi,Ricardo; Oetterer,Marília.
The use of fish silage as a substitute for protein ingredients in rations for aquatic organisms is an alternative to solve sanitary and environmental problems caused by the lack of adequate disposition for the waste from the fish industry. Besides, it is also a way of decreasing feeding costs, and, consequently, fish production costs, since feeding corresponds to about 60% of the overall expenses with production. The objective of this review was to discuss the use of fish waste, the elaboration of chemical silage and the use of this ingredient in feed for aquaculture.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish waste; Chemical silage; Feed for aquaculture.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500016

Effect of nutrients on total lipid content and fatty acids profile of Scenedesmus obliquus
Provedor de dados: 52
Autores: Darki,Behrouz Zarei; Seyfabadi,Jafar; Fayazi,Sima.
ABSTRACT  The effect of nutrients on the total lipid content and fatty acid profile of Scenedesmus obliquus isolated from the south coast of the Caspian Sea was evaluated. The nutritional compositions of the media impacted the growth rate and biomass of S. obliquus that ranged from 0.175 day-1 to 0.209 day-1and 0.92 gr·l-1 to 1.79 gr·l-1, respectively. The alga grew better in the medium which was characterized by higher levels of sodium and trace elements such as Fe, Mn, Mo, and Co and poor in N and P as compared with the other media. The highest level of the total lipid (32%) and the highest values of saturated fatty acids, in particular palmitic acid also were positively correlated with these nutrients. Peaks in polyunsaturated fatty acids (43.7 %),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monounsaturated fatty acids; Polyunsaturated fatty acids; Canonical correspondence analysis; Green algae; Caspian Sea.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100200

Evaluation of Plant Residues on the Mobility of Surface Applied Lime
Provedor de dados: 52
Autores: Miyazawa,Mário; Pavan,Marcos A.; Franchini,Júlio C.
A baixa mobilidade do calcário aplicado na superfície do solo diminui sua eficiência na redução da acidez subsuperficial. Foram estudados os efeitos de alguns resíduos orgânicos sobre a mobilidade do calcário calcítico aplicado na superfície do solo em colunas de PVC (60 cm de altura por 15 cm de diâmetro). Avaliaram-se resíduos de aveia preta, trigo, centeio, mucuna cinza e leucena na dose equivalente a 40 Mg ha-1. Adicionaram-se os resíduos vegetais e o calcário na superfície do solo e, após um programa de irrigação equivalente a 1500 mm de chuva por ano, avaliaram-se o pH e os teores de Ca, Mg, K e Al trocáveis no solo e solúveis na água efluente. O efeito do calcário sem resíduos vegetais limitou-se aos primeiros 10 cm de profundidade. Os resíduos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subsoil acidity; Ca leaching; Green manure.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300001

Mechanical Behavior of Concrete Modified by Replacement of Cement by Rice Husk Ash
Provedor de dados: 52
Autores: Saravanan,M.M.; Sivaraja,M.
ABSTRACT Construction industry is in need of lump sum quantities of materials which has increased both their demand and price. The use of large quantities of cement leads to increasing CO2 emission and as a consequence, the greenhouse effect. Consumption of wastes and byproducts from various sources in the manufacture of concrete has gained a great deal of importance in present days. Various researches are currently being conducted concerning the use of such products in concrete. RHA is a carbon neutral green product. Lots of ways are being thought of for disposing them by making commercial use of this. Rice husk ash is a good super-pozzolan which can be used to make special concrete mixes. The rice husk ash has been taken for this present study due to its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Husk ash; Strain; Compressive; Split tensile; Flexural.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300608

Qualitative and quantitative lymphoscintigraphy in the evaluation of lower limbs lymphedema
Provedor de dados: 52
Autores: Dalia,Roberta Maria; Martins,Gláucia Ribeiro P.; Barbosa,Rodolfo; Lima,Carla Flávia de; Siqueira,Cristiano Ferrari.
The purpose of this study was to prove the efficiency of qualitative and quantitative lymphoscintigraphy in the diagnosis of the lower extremity lymphedema. Seventy-seven patients had been studied, most of then (85.7%) were female. Patients’ ages ranged from 18 to 82 years. All patients underwent a bilateral lymphoscintigraphy. Images were recorded with a dual-detector instrument in the whole-body scanning mode. Feet and inguinal static views were acquired within 15 minutes and three hours after radiopharmaceutical injection. Two nuclear doctors evaluated the lymphoscintigrams qualitatively. Quantitative studies of the radiopharmaceutical absorption and the lymph node chain uptake were also obtained. Of the 154 evaluated members, 21.44% (n = 33) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphedema; Lymphoscintigraphy; Lower limbs; Dextran; Radiopharmaceutical.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700023

Comparative study of the effects of the Ga-As (904 nm, 150mW) laser and the pulsed ultrasound of 1 MHz in inflammation of tibialis muscle of Wistar rats
Provedor de dados: 52
Autores: Pinto,Marcus Vinícius de Mello; Costa,Daniel Almeida da; Rocha,Lamara Laguardia Valente; Santos,Hélio Ricardo dos; Silva,André Luis dos Santos; Barbosa,Luis Guilherme; Reis,João Batista Alves dos; Bernardo-Filho,Mario.
This paper aims to compare the therapeutic effect of the laser As-Ga of 904nm and pulsed Ultrasound of 1 MHz applied in striated skeletal muscle of inflamed rats. The animals received an intramuscular injection of bupivacaine hydrochloride in tibialis muscle in order to induce the inflammatory process, and after 24 hours, the time was considered 0 for the initiation of therapy, using a laser and ultrasound. Samples collected the muscles of the animals were stained with Hematoxylin-Eosin and histological sections of the groups used for the analysis of the muscle tissue in relation to reducing the inflammatory process, comparing the results of the two therapies used. In this study it is suggested that both treatment with laser as with ultrasound can act as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lasers; Ultrasound; Inflammation; Rats.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700037

Reproductive parameters of Lonomia obliqua Walker, 1855 (Lepidoptera: Saturniidae) in laboratory
Provedor de dados: 52
Autores: Lorini,Lisete Maria; Rebelato,Gisele Sana; Bonatti,Juliano.
Lonomia obliqua is an insect that has urticant spines in the larval stage. This species may cause death as a result of haemorrhages caused by a toxin released from the caterpillar's spines onto the skin of the victim. Since 1989 when this species was identified in Rio Grande do Sul, Brazil, many accidents have happened. The aim of this study was to verify the oviposition, fecundity, fertility, viability of eggs and incubation period of the species. Adults were left in a moth's cage to mate and lay eggs. Thirteen couples were observed daily, and the number of eggs and the caterpillars emerged were recorded. The results showed a mean of 2.8 (± 1.3) ovipositions, a mean fecundity of 135.3 (± 54.4) eggs/female, a mean fertility of 111.9 (± 55.4) eggs/female, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lonomia obliqua; Urticant caterpillar; Public health; Fecundity; Fertility.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400011

Optimization of an Activity Assay of Coprinus Cinereus Peroxidase
Provedor de dados: 52
Autores: Dong,Bing Xue; Zhang,Yu Hong; Li,Gang; Fang,Jin Tao; Zheng,Ji Huan; Mao,Run Qian.
ABSTRACT To seek a simple, rapid and sensitive Coprinus cinereus Peroxidase (CIP) activity assay, a convenient one-factor-at-a-time (OFAT) method and a response surface methodology (RSM) were used. The recombinant CIP expressed in Pichia pastoris was purified with the Ni-NTA spin column. Based on the results of catalytic efficiency (kcat/Km) analysis, 2,2'-azinobis (ethylbenzthiazoline -6-sulfonate) (ABTS) was selected as the optimal enzyme substrate. Results of the OFAT method showed that enzymatic reaction performed in 0.1 mol/L sodium acetate (pH 5.0) buffer in a 200-µl reaction mixture containing 0.5 mmol/L ABTS, 10 mmol/L hydrogen peroxide (H2O2), 49.7 ng CIP at 25°C gave an average CIP activity of 88 U/mL. The ABTS and H2O2 concentrations were then...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity assay; One-factor-at-a-time (OFAT) method; Response surface methodology (RSM); Coprinus cinereus peroxidase (CIP).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100421

Cloning and Functional Assessment of the Recombinant Human Hepcidin-25 in the Baculovirus Expression System
Provedor de dados: 52
Autores: Yazdani,Yaghoub; Keyhanvar,Neda; Tabaraei,Alijan.
Hepcidin is the primary regulatory hormone responsible for lowering the iron content in the blood circulation. Due to its biodegradability and low cytotoxicity, hepcidin is considered as an alternative for iron chelators. The baculovirus expression system may be suitable for human hepcidin production because the expressed proteins generally exhibit proper folding, post-translational modifications, and oligomerization. Using data from two vector maps, pFastBac1 and pFastBac HTB, a unique vector was designed encoding human hepcidin-25 as fusion recombinant peptide. Expression analysis showed that it was expressed as a peptide with a molecular weight near to 5 kDa. After purification and TEV treatment, findings revealed that recombinant human hepcidin-25 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepcidin-25; Iron metabolism; SF-9 expression system.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100090

Erratum:Contribution of the IAM Pathway to IAA Pool in Developing Rice Grains
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100906

Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation
Provedor de dados: 52
Autores: Guelfi,Marieli; Maioli,Marcos Antonio; Tavares,Marco Aurélio; Mingatto,Fábio Erminio.
ABSTRACT The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fipronil; Hepatotoxicity; Bioenergetics; Biotransformation; Calcium homeostasis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600843

Chromosomal studies of five species of the marine fishes from the Paranaguá Bay and the karyotypic diversity in the marine teleostei of the Brazilian coast
Provedor de dados: 52
Autores: Cipriano,Roger Raupp; Fenocchio,Alberto Sérgio; Artoni,Roberto Ferreira; Molina,Wagner; Noleto,Rafael Bueno; Kantek,Daniel Luis Zanella; Cestari,Marta Margarete.
In this study, five species of marine fishes from the Paranaguá Bay in the Brazilian coast were evaluated. Eucinostomus argenteus and Diapterus rhombeus (Gerreidae) presented 48 chromosomes, all of which more acrocentric (FN = 48); Strongylura timucu and S. marina (Belonidae) also presented 48 chromosomes, but with a higher karyotypic complexity than the Gerreidae, 10M+2SM+36A (FN = 60) and 4M+44A (FN = 52), respectively. The fifth species, Mugil curema (Mugilidae), different than the others, presented only 28 chromosomes 20M+4ST+4A (FN = 48). The species presented diversity in the karyotypic macro-structure, which should be relevant for the cytotaxonomy and the evolution of this group of the vertebrate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine fishes; Karyotype; Cytotaxonomy; Karyotypic evolution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200010

Probing the Pharmacological Parameters, Molecular Docking and Quantum Computations of Plant Derived Compounds Exhibiting Strong Inhibitory Potential Against NS5 from Zika Virus
Provedor de dados: 52
Autores: Rasool,Nouman; Jalal,Amir; Amjad,Adnan; Hussain,Waqar.
ABSTRACT Zika virus (ZIKV) is known for microcephaly and neurological disease in humans and the nonstructural proteins of ZIKV play a fundamental role in the viral replication. Among the seven nonstructural proteins, NS5 is the most conserved and largest protein. Two major functional domains of NS5 i.e. methyltransferase (MTase) and RNA-dependent RNA polymerase (RdRp) are imperative for the virus life cycle and survival. The present study explicates the inhibitory action of phytochemicals from medicinal plants against NS5 from ZIKV, leading to the identification of potential inhibitors. The crystal structure of the protein is retrieved from RCSB protein data bank. A total of 2035 phytochemicals from 505 various medicinal plants are analysed for their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ZIKV-NS5; Phytochemicals; Molecular Docking; DFT calculations.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100324

Semen and reproductive parameters during some abstinence periods after cigarette smoke exposure in male rats
Provedor de dados: 52
Autores: Sankako,Michele Kimie; Garcia,Patricia Carvalho; Piffer,Renata Carolina; Pereira,Oduvaldo Câmara Marques.
Cigarette smoking is very widespread globally and can also be implicated in male and female infertility. This study aimed to evaluate the testicular function throughout a complete spermatic cycle during abstinence from cigarette smoke exposure in order to identify a possible residual damage and whether the parameters could recover spontaneously. Male Wistar rats were randomly divided into control and cigarette smoke-exposed (20 cigarettes/day/2 months) groups. After finishing the treatment, according to the number of days after the last cigarette exposure (0, 15, 30, or 60 days), the rats were euthanized and analyzed for compromised sperm count and quality. Results showed residual damage on sperm concentration, motility and morphology; the recovery of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cigarette; Semen; Spermatozoa; Testicular Function; Male Rat.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100012

Analytical validation of LAL kinetic assay for detection and quantification of endotoxins in measles's vaccine diluents
Provedor de dados: 52
Autores: Guimarães,Rosane Cuber; Leal,Alaide Aline Xavier.
Method validation is the process used to confirm that the analytical procedures employed for a specific test is able to produce reliable and replicable results. An analytical method should be conducted in order to demonstrate that it is suitable for its intended use [1]. The aim of this present work is to demonstrate and evaluate the suitability of method validation. The analytical validation discussed herein was conducted by prescribed protocol, using 3 different batches of measles's vaccine diluents. All of the stipulated validation parameters: linearity, repeatability, reproducibility (intermediate precision) and accuracy were met. Additionally, this study demonstrated the method's capability for the determining endotoxin levels in measles's vaccine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioanalytical method; Endotoxin; Validation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200010

Production of CO2 in crude oil bioremediation in clay soil
Provedor de dados: 52
Autores: Baptista,Sandro José; Cammarota,Magali Christe; Freire,Denize Dias de Carvalho.
The aim of the present work was to evaluate the biodegradation of petroleum hydrocarbons in clay soil a 45-days experiment. The experiment was conducted using an aerobic fixed bed reactor, containing 300g of contaminated soil at room temperature with an air rate of 6 L/h. The growth medium was supplemented with 2.5% (w/w) (NH4)2SO4 and 0.035% (w/w) KH2PO4. Biodegradation of the crude oil in the contaminated clay soil was monitored by measuring CO2 production and removal of organic matter (OM), oil and grease (OandG), and total petroleum hydrocarbons (TPH), measured before and after the 45-days experiment, together with total heterotrophic and hydrocarbon-degrading bacterial count. The best removals of OM (50%), OandG (37%) and TPH (45%) were obtained in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biostimulation; Crude oil; Clay soil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400031

Effect of CLA supplementation to low-protein diets on the growth performance, carcass characteristics, plasma urea nitrogen concentration, and fatty acid profile in the meat of pigs
Provedor de dados: 52
Autores: Martínez-Aispuro,Manuel; Figueroa-Velasco,José Luis; Zamora-Zamora,Vicente; Cordero-Mora,José Luis; Narciso-Gaytán,Carlos; Sánchez-Torres,María Teresa; Carrillo-Domínguez,Silvia; Castillo-Domínguez,Rosa María.
To analyze the effect of conjugated linoleic acid (CLA) on the meat of pigs (0,1%) and three crude protein (CP) levels (nursery: 20.5, 16.0, 14.5%; growing: 16, 14.5, 11.5%; and finishing: 14.0, 12.5, 11% CP), studies were conducted with 36 hybrid (Yorkshire×Landrace×Duroc) barrows (17.3-83.5 kg), which were individually penned and allotted in a completely randomized design in a factorial (2×3) arrangement for 84 d. The analysis by phases indicated that CP level affected some variables. Average daily gain, average daily feed intake, fat free lean gain, backfat thickness, longissimus muscle area and final body weight were reduced (P≤0.05) feeding the lowest CP diet in nursery and growing pigs. Plasma urea nitrogen concentration was also lower (P≤0.05) in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pigs; Low-protein diets; Conjugated linoleic acid; Fatty acid profile in meat.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500742

Physico-chemical and cooking characteristics of some rice varieties
Provedor de dados: 52
Autores: González,Rolando J.; Livore,Alberto; Pons,Brigitte.
Some physicochemical and cooking characteristics corresponding to different varieties and commercial samples of rice were analysed. Some mechanical properties determined by both, the viscoelastograf and the Instrom Universal Food Testing Machine were also analysed. Gelatinization time (Gt), gelatinization endotherm and amylose content were determined. The results showed that there was a very good correlation between gelatinization temperature and Gt, while no noticeable correlation among others phsycochemical characteristics was observed. Regarding the mechanical properties, both methods permited to show significant differences among most of the samples. However, when the samples order obtained with hardness (Instrom) values was compared with that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice quality; Amilose; Hardness; Firmness; Cooking time; Gelatinisation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100010

Comparison of fatty acid content of fresh and frozen fillets of rainbow trout (Oncorhynchus mykiss) Walbaum
Provedor de dados: 52
Autores: Chávez-Mendoza,Celia; García-Macías,José Arturo; Alarcón-Rojo,Alma Delia; Ortega-Gutiérrez,Juan Ángel; Holguín-Licón,Celia; Corral-Flores,Gabriela.
The aim of this study was to evaluate and compare the percentage content of fatty acids (FA) of 54 rainbow trout fresh and frozen fillets. Frozen fillets were stored at -15ºC for 45 and 90 days. Saturated (SFA), monounsaturated (MUFA), polyunsaturated (PUFA), omega-6 (n-6) and omega-3 (n-3) fatty acids were determined by gas chromatograph coupled to mass spectrometry. The results indicated that during frozen storage, SFA and MUFA content increased by 32.63 and 9.25%, respectively, while PUFA content decreased by 25.3%, n-6 by 12.4% and n-3 by 32.55%. These changes were more significant (P ≤ 0.05) during the first 45 d of storage. It was concluded that the frozen storage had a negative impact on meat quality of rainbow trout due to the reduction of PUFA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Frozen storage; Rainbow trout; Fatty acid profile.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100015

Implementation of a Photovoltaic Panel to Supply Electric Cars Energy Demands
Provedor de dados: 52
Autores: Silva,Jardel Eugenio da; Santos,Francielle Rocha; Kaltmaier,Günther; Urbanetz Junior,Jair.
ABSTRACT The gradual shifting of the energy matrix to renewable sources is one of the main global strategies for sustainable development. According to studies, the use of electric energy by automobiles would lead to a reduction in gasoline consumption of 40.7% in 2031, accompanied by an increase in electricity consumption of 42.1% in relation to official projections. However, the gradual and constant dissemination and use of electric vehicles, a new paradigm emerges, which is the need to supply the energy demand of this new charge in the electric system The use of solar radiation to generate electricity to meet this new demand presents a sustainable alternative. From these questions, a structure was designed with the installation of a photovoltaic solar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic energy; Electric car.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200226

Numerical study of n-pentane separation using adsorption column
Provedor de dados: 52
Autores: Silva,Adriano da; Mariani,Viviana Cocco; Souza,Antônio Augusto Ulson de; Souza,Selene Maria de Arruda Guelli Ulson.
This work simulated numerically the n-pentane separation of a mixture of iso-pentane, n-pentane and nitrogen, using an adsorption column with zeolite 5A. The mathematical model equations of the mass and heat transfer in the adsorption column are presented, as well as the boundary and initials conditions, beyond some hypotheses and considerations. The Volume Finite Method was used in the discretization of the equations to get the system of algebraic equations and posterior development of the computational algorithm. The numerical results using the Differencing Central (CDS) and Upwind (UDS) interpolations were compared with experimental results found in the literature. The influence of the partial pressure in the adsorption column performance was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adsorption column; Mass transfer; Finite volume method.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400033

The tritrophic system hyptis suaveolens (Lamiaceae) - agromyzid leafminers (Diptera: Agromyzidae) - parasitoids (Hymenoptera): effects of herbivore density, host plant patch size, and habitat complexity on parasitism rate
Provedor de dados: 52
Autores: Queiroz,Jarbas Marcal de; Garcia,Maria Alice.
In this study a tritrophic system was evaluated to measure the contributions of the insect host density and its host plant concentration in simple and diversified habitat on the rate of parasitism. The system was composed of the plant Hyptis suaveolens, two species of agromyzid leafminers, and three morphospecies of parasitoids. The parasitism rate, patch size, and habitat complexity were found to be interdependent. If only the habitat complexity or patch size was take into account the differences in parasitism rate are not significant, although habitat diversity seemed to contribute more than the host plant concentration to increase the parasitism rate. The leaves or plants with different number of mines were equally attacked by the parasitoids. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Habitat heterogeneity; Calycomyza; Chrysocharis; Biological control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300008

Comparative evaluation of anthelmintic and antibacterial activities in leaves and fruits of Garcinia cambogia (Gaertn.) desr. and Garcinia indica (Dupetit-Thouars) choisy
Provedor de dados: 52
Autores: Tharachand,C.; Selvaraj,C. Immanuel; Abraham,Z.
This study aimed to evaluate the in vitro anthelmintic activity and antibacterial activity of the extracts from the leaves and fruits of Garcinia indica (Dupetit-Thouars) Choisy and Garcinia cambogia(Gaertn.) Desr. using the Indian earthworm Pheretima posthuma. Two concentrations (25 and 50 mg/mL) of various extracts such as petroleum ether, chloroform, ethyl acetate, methanol and water were tested. Albendazole at the concentrations of 25 and 50 mg/mL was used as the standard reference. Significant anthelmintic effects of the fruits and leaves of G. cambogia and G. indica (P&lt;0.05) were observed and the results were expressed in terms of paralysis and death time. All the extracts showed the dose dependent paralysis and death of earthworms. Among all the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthelmintic; Antibacterial; Garcinia cambogia; Garcinia indica; Minimal Inhibitory Concentration.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300379

Evaluation of the phagocitary capacity of the mononuclear system in an experimental model of obstructive jaundice employing Tc- 99m Escherichia coli
Provedor de dados: 52
Autores: Diniz,Simone Odília Fernandes; Barbosa,Alfredo José Afonso; Araújo,Ivana Durval; Nelson,David Lee; Machado,Leonardo Augusto da Silva; Cardoso,Valbert Nascimento.
Infections and endotoxemia continue to be the principal causes of morbidity and mortality of patients with obstructions of the bile duct. The objective of the present work was the investigation of the phagocitary capacity of the mononuclear system in an experimental model of obstructive jaundice utilizing Tc-99m E.coli. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were significantly higher in jaundiced rats than in the control animals (p &lt; 0.001). The liver of the jaundiced animals presented a significant reduction in Tc-99m E.coli uptake when compared to the controls (p &lt; 0.001). The data also showed that there was a significant increase in the uptake of Tc-99m E.coli by the lungs of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mononuclear phagocyte system; Technetium-99m E. coli; Obstructive jaundice.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700005

Oxidative stress during rehabilitation from protein malnutrition associated with aerobic exercise in rats
Provedor de dados: 52
Autores: Prada,Francisco José Andriotti; Macedo,Denise Vaz; Mello,Maria Alice Rostom de.
This study was designed to evaluate biomarkers of oxidative stress in rats with or without aerobic exercise during recovery from protein malnutrition. From the 30th to the 90th day of life, male Wistar rats were fed a low protein diet (LP + 6%) followed by a normal protein diet (NP = 17%) until the 120th day and separated in two groups: sedentary (S) and exercise trained (E = swimming 1h/day, 5 days/week, with from the 90th to the 120th day). Rats fed a normal protein diet were used as controls. Results showed that physical exercise had beneficial effects on body weight gain during nutrition rehabilitation. Erythrocytes catalase and glutathione reductase (biomarkers of the antioxidant system) were significantly reduced in all groups in comparison to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein malnutrition; Nutritional recovery; Exercise; Oxidative stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100006

Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers
Provedor de dados: 52
Autores: Motta,Ludymila Brandão; Soares,Taís Cristina Bastos; Ferrão,Maria Amélia Gava; Caixeta,Eveline Teixeira; Lorenzoni,Rodrigo Monte; Souza Neto,José Dias de.
The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea Arabica; Coffea canephora; Genetic diversity; Molecular markers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500728

Replacement of corn silage with cassava foliage silage in the diet of lactating dairy cows: milk composition and economic evaluation
Provedor de dados: 52
Autores: Santos,Geraldo Tadeu dos; Modesto,Elisa Cristina; Souza,Nilson Evelázio de; Ítavo,Luís Carlos Vinhas; Jobim,Clóves Cabreira; Kazama,Daniele Cristina da Silva; Valloto,Altair Antônio; Massuda,Ely Mitie.
This study aimed to evaluate the effect of corn silage (CS) replacement by cassava's foliage silage (CFS) on the production and quality of milk. Twelve lactating dairy cows were used in a randomized block experimental design with four treatments and three repetitions per block. CS was replaced by CFS at the levels of 0, 20, 40, and 60%. The replacement of CS by different levels of CFS had very low effect on the variables studied. Milk density decreased when the replacement level was increased. Fatty acids 4:0 and 6:0 presented a quadratic behavior, while fat acids 8:0, 10:0, and 15:0 presented a linear behavior as their concentrations fell when CFS diet content was increased. The CS replacement by 20% until 60% CFS resulted a significant decrease in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal production; Economic evaluation; Fatty acid profile; Milk production; Milk quality.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700033

Selection for durable resistance to leaf rust using test-crosses on IAPAR-59 and Tupi IAC 1669-33 cultivars of Coffea arabica
Provedor de dados: 52
Autores: Sera,Gustavo Hiroshi; Sera,Tumoru; Ito,Dhalton Shiguer; Azevedo,José Alves de; Mata,João Siqueira da; Doi,Deisy Saori; Ribeiro Filho,Claudionor.
The aim of this study was to identify plants of the IAPAR-59 and Tupi IAC 1669-33 coffee cultivars with less defeated resistance genes by the rust races present at IAPAR (Londrina, Paraná State, Brazil) using test-crosses. Eighteen test-crosses derived from hybridizations between 'IAPAR-59' or 'Tupi IAC 1669-33' with susceptible coffee to the rust disease were evaluated. Six hybrids were used as susceptible standards originated from hybridizations between two susceptible coffee plants. Many parental plants of the 'IAPAR-59' and 'Tupi IAC 1669-33' presented more defeated resistance genes against rust races present at IAPAR than others of these cultivars or the genes were in heterozygous, because of segregant susceptible plants observed in some test-crosses....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemileia vastatrix; Breeding; Coffee crop; Rust races; SH genes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400001

Short term fluctuations and succession trends in tropical floodplain vegetation measured by correspondence analysis
Provedor de dados: 52
Autores: Santos,Anderson Medeiros dos; Thomaz,Sidinei Magela.
The aim of this work was to study the aquatic macrophytes community development to distinguish the fluctuations from succession trends at different temporal and spatial scales in the Upper Paraná River floodplain. The samples were collected quarterly from May 2000 to March 2002 in seven lakes with different degrees of connectivity from the main river channel and analyzed by between-class correspondence analysis. It was difficult to establish any pattern of community development at local scale but at a higher level of organization, disconnected lakes were characterized by fluctuation of the floristic composition. Connected lakes showed a straight trajectory, indicating that succession was occurring at these sites. Probably the income of propagules and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic macrophytes; Correspondence Analysis; Paraná River; Succession; Tropical floodplain.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400016

Spatial and temporal distribution of ichthyoplankton in the upper Uruguay river, Brazil
Provedor de dados: 52
Autores: Hermes-Silva,Samara; Reynalte-Tataje,David; Zaniboni-Filho,Evoy.
The distribution and abundance of fish eggs and larvae was analyzed in three sections of the Upper Uruguay river, in a stretch of 290 km. Samples were collected monthly from October, 2001 to March, 2002 during 48-h cycles at 6-h intervals between each sampling. Surface and bottom samples were collected with a 0.5-mm mesh cylindroconical net. Fishes from the Upper Uruguay river were reproductively active mainly from October to January, and this activity was more intense at the Ligeiro and Chapecó tributaries and Chapecó main river. It was observed that the tributaries are important spawning grounds and larval nursery sites, indicating the importance of preserving such environments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ichthyoplankton; Fish eggs and larvae; Uruguay river; 48-h cycles.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400017

Morphological markers for microspore developmental stage in maize
Provedor de dados: 52
Autores: Moraes,Ana Paula de; Bered,Fernanda; Carvalho,Fernando Irajá Felix de; Kaltchuk-Santos,Eliane.
The use of maize in anther culture has been limited because only few genotypes presented a high androgenetic potential. Obtaining the proper stage of microspore development at culture initiation is one of the most crucial factors for success in the androgenesis. For Brazilian maize genotypes there are no studies reporting a correlation between cytological features and morphological parameters. In this study, morphological parameters were recorded and associated with cytological specific stages of the the microsporogenesis in two Brazilian maize genotypes that were sowed in different places (field and growing chamber). For both genotypes, the plants of the growing chamber presented a delay in development. Spikelets length and anther length/spikelet length...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androgenesis; Anther culture; Microsporogenesis; Zea mays.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500006

Contribution of forest species to the floristic composition of a forested savanna in southeastern Brazil
Provedor de dados: 52
Autores: Pinheiro,Marcelo Henrique Ongaro; Monteiro,Reinaldo.
Based on studies of floristic lists the contribution of forest tree and shrub species in a forested savanna area in the city of Bauru, State of São Paulo, and in 14 different places sheltering cerrado sensu stricto and cerradão in Brazil is defined as the evidence of forest species causing a change of cerrado heterogeneity. The percentages of occurrence of forest species in the locations analyzed varied from zero to 4.5%. The proximity of different forest matrices, the occurrence of fires and the inclusion criteria used were some of the factors that may have influenced such variation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecotone; Generalist species; Exchange of floristic elements; Facilitation; Microhabitats.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600011

Kinetics and Thermodynamics of Thermal Inactivation of β-Galactosidase from Aspergillus oryzae
Provedor de dados: 52
Autores: Klein,Manuela Poletto; Sant’Ana,Voltaire; Hertz,Plinho Francisco; Rodrigues,Rafael Costa; Ninow,Jorge Luiz.
ABSTRACT For optimization of biochemical processes in food and pharmaceutical industries, the evaluation of enzyme inactivation kinetic models is necessary to allow their adequate use. Kinetic studies of thermal inactivation of β-galactosidase from Aspergillus oryzae were conducted in order to critically evaluate mathematical equations presented in the literature. Statistical analysis showed that Weibull model presented the best adequacy to residual enzymatic activity data through the processing time and its kinetic parameters as a function of the temperature, in the range of 58-66 ºC. The investigation suggests the existence of a non-sensitive heat fraction on the enzyme structure, which is relatively stable up to temperatures close to 59 ºC....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-Galactosidase; Enzyme Inactivation; Modelling; Protein Denaturation; Weibull.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100409

Spray-dried tomato powder: reconstitution properties and colour
Provedor de dados: 52
Autores: Sousa,Alexandre Santos de; Borges,Soraia Vilela; Magalhães,Natália Ferreira; Ricardo,Hevandro Vaz; Azevedo,Aline Damico.
Powdered tomato was produced by spray drying the tomato pulp. A full 2³ factorial design with the central point was used, varying the feed flow rate (127-276 g/min), air inlet temperature (200-220ºC) and the atomisation speed (25,000-35,000 rpm). The responses analysed were: moisture content, solubility, wettability consistency and colour, but the factors only significantly affected the colour parameter. All the samples became significantly darker and less red with an increase of the variables under study. A low atomisation speed (25,000 rpm) and lower inlet air temperature (220ºC) produced the powders with a higher colour index (a/b) and less darkening.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spray dried tomato (Lycopersicon esculentum); Colour; Solubility; Wettability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400019

Microbial Diversity: Relevance and Relationship Between Environmental Conservation And Human Health
Provedor de dados: 52
Autores: Panizzon,Jeremias Pakulski; Pilz Júnior,Harry Luiz; Knaak,Neiva; Ramos,Renata Cristina; Ziegler,Denize Righetto; Fiuza,Lidia Mariana.
This work presents bibliographic data on the role and function of microbial diversity. The increasing use of probiotics and prebiotics foods has led to the studies on their actual functions in the human body. It is known that in the environment, microorganisms are extremely important in recycling of nutrients, balance of trophic chains, vital physiological activities in the plants and animals, as well as the conservation of natural habitats. In human food, these microscopic organisms contribute from flavoring products to the synthesis of antimicrobial substances and vitamins essential to living beings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Nutraceutical; Collective health; Review.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100137

Intestinal parasites in ecotourism region of the state of Paraná, Brazil
Provedor de dados: 52
Autores: Falavigna,Dina Lúcia Morais; Almeida,Amanda Andrea de; Iwazaki,Renata Sayuri; Araújo,Silvana Marques de.
To determine the indices of prevalence of intestinal parasites and commensals in the residents of ecotourism region of Paraná State, a retrospective study was conducte, from January/2003 to December/2004 on 3,764 fecal parasitological examinations performed by a private laboratory of Ubiratã using the Baermann-Moraes, water-sedimentation and Willis methods. Among the individuals aged eight months to 89 years, 522 (13.9%) cases were positive. Giardia lamblia (175/522; 24.2%) was the most prevalent parasite (p&lt;0.000). Entamoeba coli was the most common commensal, with 238/552 (32.9%) cases (p&lt;0.000), being frequently associated with other species, either parasitic or commensal. The children of preschool age (2-6 years old) and school age (7-14 years)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal Parasites; Ecotourism; Travelers Parasitology; Paraná.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400007

Pentoses Used in Cultures of Synechococcus nidulans and Spirulina paracas: Evaluation of Effects in Growth and in Content of Proteins and Carbohydrates
Provedor de dados: 52
Autores: Freitas,Bárbara Catarina Bastos de; Cassuriaga,Ana Paula Aguiar; Morais,Michele Greque de; Atala,Daniel Ibraim Pires; Costa,Jorge Alberto Vieira.
Abstract The biological assimilation of the sugars present in lignocellulosic residues has gained prominence since these residues are the most abundant and economic residues in nature. Thus, the objective of this work was to determine whether the use of D-xylose and L-arabinose as sources of carbon in Synechococcus nidulans and Spirulina paracas cultures affects the growth and production of proteins and carbohydrates. Kinetic growth parameters, pentose consumption, protein content and carbohydrates were evaluated. Synechococcus nidulans and Spirulina paracas consumed all concentrations of pentose used. The highest cellular concentration (1.37 g.L-1) and the highest protein productivity (54 mg.L-1.d-1) were obtained for Spirulina paracas, which was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabinose; Proteins; Spirulina; Synechococcus; Xylose.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100513

Investigation of the tolerability of oral stevioside in Brazilian hyperlipidemic patients
Provedor de dados: 52
Autores: Silva,Gisleine Elisa Cavalcante da; Assef,Abdol Hakin; Albino,Claudio Cordeiro; Ferri,Letícia de Araujo Funari; Tasin,Gilson; Takahashi,Mirian Hideko; Eik Filho,Wilson; Bazotte,Roberto Barbosa.
The tolerability of stevioside (2.75 mg/kg/day) obtained from leaves of Stevia rebaudiana (Bert) Bertoni (Compositae) was investigated in hyperlipidemic patients. For this purpose a placebo controlled double blind study was performed. The patients were randomized in two groups: the first group received capsules containing placebo and the second group received capsules containing stevioside (50 mg) during 90 days. All capsules were ingested twice daily, i.e., 2 capsules before lunch and 2 capsules before dinner. After the selection of the patients and each 30 days body mass index and laboratory tests (alanine aminotransferase, aspartate aminotransferase, gamma-glutamyltransferase, total cholesterol, high density lipoprotein, low density lipoprotein, very...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stevia rebaudiana (Bert) Bertoni; Stevioside; Clinical investigation; Placebo effect.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500007

Application of Next-generation Sequencing in Clinical Molecular Diagnostics
Provedor de dados: 52
Autores: Seifi,Morteza; Ghasemi,Asghar; Raeisi,Sina; Heidarzadeh,Siamak.
ABSTRACT Next-generation sequencing (NGS) is the catch all terms that used to explain several different modern sequencing technologies which let us to sequence nucleic acids much more rapidly and cheaply than the formerly used Sanger sequencing, and as such have revolutionized the study of molecular biology and genomics with excellent resolution and accuracy. Over the past years, many academic companies and institutions have continued technological advances to expand NGS applications from research to the clinic. In this review, the performance and technical features of current NGS platforms were described. Furthermore, advances in the applying of NGS technologies towards the progress of clinical molecular diagnostics were emphasized. General advantages and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Clinical molecular applications; Ethical aspects; Next generation sequencing.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100303

Estudos Sobre Leishmaniose: I. Primeiros Casos de Leishmaniose Espontânea Observados em Cobáios
Provedor de dados: 52
Autores: Medina,Heitor.
The author describes the first cases of spontaneous leishmaniasis in guinea-pigs, as verified for the first time, in Curitiba, at the Institute of Biology and Technological Research. Studying the inoculability of Paracoccidioide brasiliensis in guinea-pigs, he found animals which presented tumoral lesions in the ears and peritesticular skin. The examination of these tumors, mixed with a small drop of Giemsa's original solution, plus the exam of the rubbings of the same material, coloured by the May-Grunwald Giemsa after fixation by Heidenhein's solution and the histological exams of the cuts in the tumoral lesions revealed the presence of elements which possessed the same morphology and aspect of the LEISHMAN-DONOVANI corpuscles. In the meanwhile, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Guinea-pig.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500003

Assesment of the Genotoxic Effect of the Diazinon on Root Cells of Allium cepa (L.)
Provedor de dados: 52
Autores: Pandir,Dilek.
ABSTRACT Organophosphorous pesticides (OPs) posses a great potential of acute toxicity for exposed animals and men. To evaluate the toxic potential of the organophosphate diazinon on root meristematic cells of Allium cepa L., was created two groups: In group 1 (control group), was not given any chemical. In group 2 (diazinon-treatment group), different doses (10, 40, 80 and 160 ppm) and times periods (24, 48 and 72 h) were administered. After exposure, cell death, effective concentration (EC50), mitotic index, cellular /chromosome aberrations, DNA damage by comet assay and RAPD-PCR were assessed at exposure times. EC50 value of diazinon was detected approximately 80 ppm. Hyperchromasia, later segragation, micronucleus, pulverised nucleus, nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diazinon; Allium test; DNA damage; Genotoxicty; RAPD-PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100406

Dispersal syndromes related to edge distance in cerrado sensu stricto fragments of central-western Brazil
Provedor de dados: 52
Autores: Jardim,André Vitor Fleuri; Batalha,Marco Antônio.
The main selective forces affecting the fruiting strategies are related to the environment in which plants occur. As a savanna, microclimatic conditions should not vary in relation to distance from edge in cerrado sensu stricto fragments. Thus, we postulated that the importance of different dispersal syndromes would not vary towards the fragment core. Our aim was to test in four cerrado sensu stricto fragments in central Brazil whether the absolute density of anemo-, auto-, and zoochorous individuals varied in relation to edge distance. According to results, the absolute density of anemo-and autochorous individuals did not vary, whereas those of zoochorous individuals increased with edge distance, pointing out that there were other factors rather than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central-western Brazil; Cerrado; Dispersal; Edge effect.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500014

Molecular systematics of the Phyllachorales (ascomycota, fungi) based on 18S ribosomal DNA sequences
Provedor de dados: 52
Autores: Wanderlei-Silva,Denise; Ramalho Neto,Eduardo; Hanlin,Richard.
In order to evaluate the monophyly of the Phyllachorales from a molecular standpoint and elucidate its phylogenetic relationships with other orders, a segment of the 18S rRNA gene from several representatives of the Phyllachorales, including species of Glomerella, Phyllachora, Coccodiella (=Coccostroma), Sphaerodothis, Ophiodothella, as well as Magnaporthe was sequenced. Maximum Parsimony analysis revealed that the Phyllachorales was a polyphyletic assemblage of taxa. None of the other members of the Phyllachorales, which produced either a clypeus or stroma, clustered with Glomerella. Of the taxa examined, was Coccodiella the closest relative of Phyllachora. Magnaporthe was closely related to the Diaporthales. Our 18S rDNA data highly supported Glomerella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA sequencing; 18S ribosomal DNA; PCR; Phyllachorales.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300002

Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells
Provedor de dados: 52
Autores: Gallão,Maria Izabel; Cortelazzo,Ângelo Luiz; Fevereiro,Manuel Pedro Salema; Brito,Edy Sousa de.
Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitin; Elicitation; Peroxidase; PAL; Sugarcane; Tissue culture.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002

An overview on molecular markers for detection of ochratoxigenic fungi in coffee neans
Provedor de dados: 52
Autores: Fungaro,Maria Helena Pelegrinelli; Sartori,Daniele.
Ochratoxin A (OTA) is one of the most abundant food-contaminating mycotoxins. Its occurrence in several agricultural commodities has been considered a worldwide problem. This toxin is mainly produced by Aspergillus species. OTA has nephrotoxic, immunosuppressive, and carcinogenic effects and consequently the contamination with this toxin represents a high risk for human and animal health. In the last 5 years, several investigators have applied molecular methods in order to develop PCR assays for identifying and quantifying OTA-producing fungi in coffee beans samples. The main objective is to allow the detection of microorganisms capable of producing OTA, preferentially prior to ochratoxin production and accumulation. In this contribution several of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxigenic fungi; Molecular marker; PCR detection; Molecular identification.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700001

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers
Provedor de dados: 52
Autores: Veasey,Elizabeth Ann; Cardin,Daruska; Silva,Rainério Meireles; Bressan,Eduardo de Andrade; Vencovsky,Roland.
To assess the genetic diversity and genetic structure parameters, nine populations of Oryza glumaepatula from the Amazon biome, four from the Pantanal biome, and one collected at Rio Xingu, Mato Grosso, totaling 14 populations and 333 individuals were studied with isozyme markers. Six loci were evaluated showing a moderate allozyme variability (A = 1.21, P = 20.7%, Ho = 0.005, He = 0.060). The populations from the Pantanal biome showed higher diversity levels than the Amazon biome. High genetic differentiation among the populations, expected for self-fertilizing species, was observed (F ST=0.763), with lower differentiation found among the Pantanal populations (F ST=0.501). The average apparent outcrossing rate was higher for the Pantanal populations (t a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Isozymes; Oryza glumaepatula; Outcrossing rate; Populations.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500001

First record of albinism in the smooth dogfish Mustelus Schimitti Springer, 1939 (Carcharhiniformes - Triakidae) from Southern Brazil
Provedor de dados: 52
Autores: Teixeira,Simone Ferreira; Araújo,Maria Lúcia Góes de.
This paper reports on a subadult male specimen of the smooth dogfish, Mustelus schimitti, caught in the continental shelf of Rio Grande Sul, during winter of 1993, which exhibited the characteristics of total albinism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albinism; Mustelus schimitti; Carcharhiniformes; Triakidae.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200017

Analysis of masseter muscle tension through electromyography among residents of the city of Buenos Aires - Argentina
Provedor de dados: 52
Autores: Pinto,Marcus Vinícius de Mello; Pereira Jr,Francisco José; Scaglione,Guilhermo Mario; Santos-Filho,Sebastião David; Silva,Cristiane Martins da; Silva,Marco Antônio Guimarães da; Rocha,Lamara Laguardia Valente; Gonçalves,Reggiani Vilela.
This study aimed at collecting data of masseter myofascial tension, related to the joint hyperactivity by electromyography activity of masseter muscle at rest, isotonic and isometric chewing. 08 patients living in the city of Buenos Aires, Argentina, volunteers, aged between 22 and 62 years, both sexes were evaluated. The electromyography data collection at rest had reported that 50% showed differences in masseter muscle tension increased in the left for the right masseter; in isometric contraction, 50% had a difference in masseter tension right; in isotonic contraction 62.5% there was a difference in increased tension in the left masseter. Anthropometry of the face was suggested, where 25% showed significant variance of 01 cm of discrepancy on the right,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle tension; Masseter; Electromyography.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200008

Phytochrome and temperature control of seed germination in Muntingia calabura L. (Elaeocarpaceae)
Provedor de dados: 52
Autores: Leite,Idalina T. de A.; Takaki,Massanori.
The temperature range of germination of seeds of Muntingia calabura L. was 15-35° C and the optimum temperature at 35° C where more seeds germinated at short period. The fluence response curves demonstrated the involvement of phytochrome in the control of germination through the low fluence response. The effect of photoperiod showed that the species need long daily exposures to white light and that shade light inhibited completely seed germination, confirming as pioneer species that colonized only large gaps in the forest.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seed germination; Phytochrome; Light; Temperature; Muntingia calabura.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300012

Effect of leaf Water potential on cold tolerance of Coffea arabica L.
Provedor de dados: 52
Autores: Caramori,Lázara Pereira Campos; Caramori,Paulo Henrique; Manetti Filho,João.
Young coffee plants from cultivar Mundo Novo of Coffea arabica were grown without irrigation for 32 consecutive days, to evaluate the effect of leaf water potential on damage caused by low temperatures, under controlled conditions. A wide range of leaf water potentials were evaluated, from - 0.45MPa (wet soil) at the beginning of the experimental period, to - 4.8MPa (severe leaf wilting) at the end. Results showed that under moderate water stress, there was a higher frequency of undamaged plants and lower frequency of severely damaged plants. These results help explain part of the regional variability observed after a frost and stress the importance of new studies associating cold and drought tolerance in coffee.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cold tolerance; Coffee; Leaf water potential; Water stress.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600006

Plants and pollinating bees in Maringá, State of Paraná, Brazil
Provedor de dados: 52
Autores: Toledo,Vagner de Alencar Arnaut de; Fritzen,Adriano Ernesto de Toledo; Neves,Carolina Antunes; Ruvolo-Takasusuki,Maria Claudia Colla; Sofia,Silvia Helena; Terada,Yoko.
The present study was carried out to survey the bees as visitors to melliferous flora in the region of Maringá, state of Paraná, Brazil. A total of 331 insects were captured, and the fauna comprised 39.88% Trigona spinipes, 38.37% Apis mellifera, 8.16% Tetragonisca angustula, 3.93% Halictidae, 1.21% Megachilidae, 2.42% Anthophoridae, and 3.32% other Hymenoptera. Eleven plant species from nine families were observed. The four families most frequently visited by A. mellifera were Pontederiaceae (93.53%), Sterculiaceae and Polygoniaceae (47.22%), Apocynaceae and Apiaceae (42.86%). The families most visited by T. spinipes were Lamiaceae (64.70%), Apocynaceae (57.14%), Sterculiaceae (51.85%) and Anacardiaceae (48.39%), and the families most visited by T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless bees; Pollination; Melliferous flora.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400025

Isolation and partial characterization of a D-galactose-binding lectin from the latex of Synadenium carinatum
Provedor de dados: 52
Autores: Souza,Maria Aparecida; Amâncio-Pereira,Francielle; Cardoso,Cristina Ribeiro Barros; Silva,Adriano Gomes da; Silva,Edmar Gomes; Andrade,Lívia Resende; Pena,Janethe Deolina Oliveira; Lanza,Henrique; Afonso-Cardoso,Sandra Regina.
A lectin from the latex of Synadenium carinatum was purified by affinity chromatography on immobilized-D-galactose-agarose and shown to be a potent agglutinin of human erythrocytes. The haemagglutination of human red cells was inhibited by 3.0 mM N-acetyl-D-galactopyranoside, 6.3 mM methyl-beta-D-galactopyranoside, 50 mM methyl-alpha-D-galactopyranoside and 50 mM D-fucose but not by L-fucose, demonstrating an anomeric and a conformational specificity. According to SDS-PAGE analysis, the lectin appeared to be a glycoprotein composed of two polypeptide chains of ca. 28 and 30 kDa, but size exclusion chromatography (Sephadex G-100) and native PAGE revealed a protein of apparent molecular weight 120 - 130 kDa made up of 28 and 30 kDa subunits. The lectin was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D-Galactose-binding; Lectin; Latex; Synadenium carinatum.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600005

Studies on the electrochemical disinfection of water containing Escherichia coli using a Dimensionally Stable Anode
Provedor de dados: 52
Autores: Gusmão,Isabel Celeste Caíres Pereira; Moraes,Peterson Bueno; Bidoia,Ederio Dino.
The aim of this work was to investigate the disinfectant effect of electrolysis on chlorine-free water, artificially contaminated with Escherichia coli (CCT-1457) and to evaluate the bactericidal activity of electrolysis and kinetic behavior of a single-cell reactor, with a DSA (Dimensionally Stable Anode) electrode to develop a scaled-up system. A high-density E. coli suspension (10(6) CFU mL-1) was electrolyzed in this reactor at 25, 50 and 75 mA cm-2 for up to 60 min, at flow rates of 200 and 500 L h-1. Bacterial survival fell by 98.9% without addition of chlorinated compounds and a power consumption rate not more than 5.60 kWh m-3 at flow rate of 200 L h-1 and 75 mA cm-2. The process produced a germicidal effect that reached this inactivation rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E. coli inactivation; Electrochemical treatment; Electrolysis of microorganisms; Electroxidation process; Bactericidal effect; DSA oxide electrode.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500029

Variational calculus (optimal control) applied to the optimization of the enzymatic synthesis of ampicillin
Provedor de dados: 52
Autores: Ribeiro,Marcelo Perencin de Arruda; Giordano,Roberto de Campos.
In this work, optimal control techniques were used to optimize the feed of reactants during the enzymatic synthesis of ampicillin in a semi-batch reactor. Simulation results showed that a semi-batch integrated reactor (with product crystallization) might achieve 88% 6-APA (6-aminepenicillanic acid) conversion and 92% of PGME (phenylglycine methyl ester) yield, with a productivity between 3.5 and 5.5 mM min-1.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synthesis of beta-lactam antibiotics; Fed-batch reactor optimization; Optimal control.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400003

Erratum: Regulating Effect Of Carnosine And /Or L- Arginine On The Expression Of Inflammatory Molecules Induced Nephropathy In The Hypoxic Rat Model
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100902

A chemically defined medium for production of actinomycin D by Streptomyces parvulus
Provedor de dados: 52
Autores: Queiroz Sousa,Maria de Fátima Vieira de; Lopes,Carlos Edison; Pereira Júnior,Nei.
A chemically defined medium consisting of D(+)fructose, L(-)threonine, K2HPO4, MgSO4.7H2O, ZnSO4.7H2O, CaCl2.2H2O, FeSO4.7H2O and deionized water, was developed to maximize the synthesis of actinomycin D by the Streptomyces parvulus DAUFPE 3124 strain. This medium resulted in the maximum antibiotic concentration of 133mg/L while using the original medium the production of actinomycin D was poor not surpassing 43mg/L.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinomycin D; Production; Streptomyces. parvulus; Synthetic medium.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300002

Acetylsalicylic acid and morphology of red blood cells
Provedor de dados: 52
Autores: Frydman,Jacques Natan Grinapel; Fonseca,Adenilson de Souza da; Rocha,Vanessa Câmara da; Benarroz,Monica Oliveira; Rocha,Gabrielle de Souza; Pereira,Marcia de Oliveira; Pereira,Mario José; Medeiros,Aldo Cunha; Bernardo-Filho,Mario.
This work evaluated the effect of in vitro and in vivo treatment with ASA on the morphology of the red blood cells. Blood samples or Wistar rats were treated with ASA for one hour. Blood samples or animals treated with saline were used as control group. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of red blood cells were evaluated under optical microscopy. Data showed that the in vitro treatment for one hour with ASA at higher dose used significantly (p&lt;0.05) modified the perimeter/area ratio of the red blood cells. No morphological alterations were obtained with the in vivo treatment. ASA use at highest doses could interfere on shape of red blood cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylsalicylic Acid; Blood Cells; Morphology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300010

Impact of Simulated Warming on Growthand Floral Characteristics of Two Varieties of Medicinal Epimedium
Provedor de dados: 52
Autores: Xuemei,Zhang; Zengli,Fan; Qiumei,Quan.
ABSTRACT An open top chamber (OTC) warming system was established in natural field conditions to study the impact of simulated warming on growth and floral characteristics of E. wushanense and E. acuminatum. Results: plant height and leaf growth were affected significantly.In +2°C warming condition, increment of plant height, leaf length and width and daily mean incrementof the two varieties were significantly greater than those of the control group; however, in warming +5°C condition, the increments were significantly lower than those of the control group. Floral differentiation was presented when different treatment was adopted. Floral quantitative character of E. acuminatum increased markedly after +2°C warming, but declined markedly after +5°C warming;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simulated warming; E. wushanense E. acuminatum floral characteristics growth status.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200203

Molecular characteristics, recombinant expression and activity detection of OsGSTL1 from rice
Provedor de dados: 52
Autores: Hu,Tingzhang; Yang,Yongwei; Tan,Lili; Yang,Junnian; Wu,Yingmei.
The mRNA of OsGSTL1 was detected in the roots and leaves of rice plants at seedling and tillering stages, and their roots, leaves and panicles at the heading stage. The full-length open reading frame of OsGSTL1 cDNA was 732 bp and encoded a putative polypeptide of 243 amino acids with a calculated molecular mass of 27.30 kDa and a theoretical pI of 5.50. The protein sequences of OsGSTL1 exhibited typical feature of the lambda class GST, which contained the conserved domain "GST_C_Lambda" in C-terminal alpha helical domain and a highly conserved Cys42 in active center. In silico predictions showed that the OsGSTL1 protein was strongly hydrophilic. The phylogenetic analysis revealed OsGSTL1 belonged to monocots subgroup and was closer to IN2-1 of Z. may. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione S-transferase; GST activity; In silico; Oryza sativa L.; Recombinant expression; Semi-quantitative RT-PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100011

Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates
Provedor de dados: 52
Autores: Oliveira,Flávia Márcia; Ruiz-de-Souza,Valéria; Campana-Pereira,Maria Aparecida; Gontijo,Cristiano Machado.
One of the mechanisms by which adjuvants are believed to promote T-cell activation and prevent induction of oral tolerance is by up-regulating the expression of co-stimulatory molecules on antigen presenting cells. Mice treated orally with palmitoyl-ovalbumin conjugates become immunized, while those treated with native ovalbumin (Ova) become tolerant. Cells from the peritoneal cavity of B6D2F1 mice were cultured in the presence of 0.01, or 0.1 mg/100ml of either Ova, or palmitoyl-Ova and tested for the presence of cell markers. PE-conjugated anti-mouse CD80, CD86, and CD11b antibodies as well as biotin-PE were used to stain the antigen-activated peritoneal cells. A significant increase in the expression of CD86 and CD80 was observed following in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovalbumin; Palmitic Acid; Cellular Immunity; CD86; CD80; CD11b.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600011

Antibacterial activity of chitosan and the interpolyelectrolyte complexes of poly(acrylic acid)-chitosan
Provedor de dados: 52
Autores: Ortega-Ortiz,Hortensia; Gutiérrez-Rodríguez,Baltazar; Cadenas-Pliego,Gregorio; Jimenez,Luis Ibarra.
The antimicrobial activity of chitosan and water soluble interpolyelectrolyte complexes of poly(acrylic acid)-chitosan was studied. Chitosans of two different molecular weights were tested at different concentration for 0.5 to 5 g·L-1 as antimicrobial agents against P. aeruginosa and P. oleovorans. In both cases, the best microbial inhibition was obtained with the concentration of 5 g·L-1. However, the interpolyelectrolyte complexes of poly(acrylic acid)-chitosan with composition φ =2 produced higher antibacterial activity than the two chitosans at the concentration of 0.5 g·L-1. The NPEC2 complex was more effective than chitosans. This could be attributed to the number of moles of the amino groups of chitosan and the carboxylic acid groups of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial activity; Chitosan; Complexes; Poly(acrylic acid); Polyelectrolytes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300016

Resistance induction for Meloidogyne incognita in sugarcane through mineral organic fertilizers
Provedor de dados: 52
Autores: Chaves,Andréa; Pedrosa,Elvira Maria Régis; Pimentel,Rejane Magalhães de Mendonça; Coelho,Rildo Sartori Barbosa; Guimarães,Lílian Margarete Paes; Maranhão,Sandra Roberta Vaz Lira.
The effects of Coda Radimax (CR), Coda Humus-PK (CH) and Coda Vit (CV) on the induction of resistance for Meloidogyne incognita in sugarcane (Saccharum sp.) varieties (RB92579, RB863129, RB867515) through nematode reproduction, plant development and root anatomy analysis, emphasizing the differences of the lignin deposition, and cortex-vascular cylinder proportions were investigated. In 90 days after inoculation with eggs of M. incognita, CR reduced the number of eggs per root system in all the sugarcane varieties; CH and CV reduced eggs density in RB867515; CR increased stalk number in RB92579 and fresh weight of shoots in RB863129 and RB92579; CH increased plant height in RB92579 and fresh weight of shoots in RB92579 and RB867515, CV affected fresh weigh...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharum spp.; Phosphites; Root-knot nematode; Root anatomy; Meloidogyne.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600011

Cultivation of juvenile fat snook (Centropomus parallelus Poey, 1860) fed probiotic in laboratory conditions
Provedor de dados: 52
Autores: Barbosa,Moysés Cavichioli; Jatobá,Adolfo; Vieira,Felipe do Nascimento; Silva,Bruno Corrêa; Mourino,José Luiz Pedreira; Andreatta,Edemar Roberto; Seiffert,Walter Quadros; Cerqueira,Vinícius Ronzani.
The objective of this study was to investigate the growth of juvenile fat snook (Centropomus parallelus) in laboratory conditions when fed a diet supplemented with the probiotic Lactobacillus plantarum. Changes in the intestinal flora, haematological parameters and growth performance were assessed using 180 fishes (54.2 ± 13.4 g each). The fishes were subjected to two treatments divided into six cages: 1) feed supplemented with probiotic, and 2) feed without probiotic (control). The temperature, dissolved oxygen and salinity were maintained at 25 ± 1 ° C, 4.0 mg.L-1 and 33 ‰, respectively. After 10 weeks of culture, the fishes fed probiotic had reduced viable culturable heterotrophic bacteria and Vibrio spp. and increased lactic acid bacteria in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus plantarum; Hematology; Bacterial microbiota; Body index.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400020

Production and extraction of phytotoxins from Colletotrichum dematium FGCC# 20 effective against Parthenium hysterophorus L.
Provedor de dados: 52
Autores: Singh,Jaya; Quereshi,Sadaf; Banerjee,Nikita; Pandey,Akhilesh Kumar.
The aim of this work was to study the herbicidal potential of Cell free culture filtrate of Colletotrichum dematium FGCC#20 against Parthenium by employing different bioassays i.e. shoot-cut, seedling, detached leaf and seed germination. On solvent extraction of the Cell free culture filtrate, Ethyl acetate extracted fraction showed the presence of phytotoxic moiety.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parthenium hysterophorus; Colletotrichum dematium; Bioassay; Cell Free Culture Filtrate (CFCF); Solvent extraction; Hours post treatment (hpt).
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300023

Nuclear medicine procedures for the evaluation of male sexual organs: a brief review
Provedor de dados: 52
Autores: Bernardo-Filho,Mario; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Carter,Kevin; Missailidis,Sotiris.
Sexuality consists of three aspects that are interrelated and inseparable, biological, physiological and social. The biological aspect considers the individual's capability to give and to receive pleasure. In consequence, it covers the functionality of the sexual organs and the physiology of human sexual response cycle. Diagnostic imaging modalities, such as single photon emission computed tomography (SPECT) and positron emission tomography (PET) have been used to evaluate clinical disorders of the male reproductive system. PET and SPECT procedures basically involve the administration of a radiopharmaceutical that has a higher uptake in a specific tumor or tissue. The aim of this brief review is to present some radiopharmaceuticals that have been used in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Male sexual organs; PET; SPECT; Radiopharmaceutical; Sexuality.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700004

Antagonism of Bacillus spp. against Xanthomonas campestris pv. campestris
Provedor de dados: 52
Autores: Monteiro,Leila; Mariano,Rosa de Lima Ramos; Souto-Maior,Ana Maria.
The antagonism of eight Bacillus isolates was investigated against nine strains of Xanthomonas campestris pv. campestris (causal agent of crucifers black rot) to assess the role of lipopeptides in this process. Antimicrobial and hemolytic (surfactant) activity tests were performed in vitro using agar diffusion methods. Antibiosis and hemolysis were positive for four Bacillus isolates against all X. campestris pv. campestris strains. The correlation observed between antimicrobial and hemolytic activities indicated that lipopeptides were involved in the antibiosis mechanism of the studied antagonists. Fermentation studies were carried out with the isolates that showed highest antimicrobial and hemolytic activities, to follow up growth and production of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Biological control; Xanthomonas campestris pv. campestris; Crucifers black rot.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100004

Physicochemical and Biological Investigation of Different Structures of Carbon Coatings Deposited onto Polyurethane
Provedor de dados: 52
Autores: Kaczorowski,Witold; Batory,Damian; Jakubowski,Witold; Szymanski,Witold; Komorowski,Piotr; Walkowiak,Bogdan; Sanak,Marek; Niedzielski,Piotr.
The aim of this study was to examine the thrombogenic properties of polyurethane that was surface modified with carbon coatings. Physicochemical properties of manufactured coatings were investigated using transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS), Raman spectroscopy and contact angle measurement methods. Samples were examined by the Impact-R method evaluating the level of platelets activation and adhesion of particular blood cell elements. The analysis of antimicrobial resistance against E. coli colonization and viability of endothelial cells showed that polyurethane modified with use of carbon layers constituted an interesting solution for biomedical application.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diamond-like carbon; Atomic force microscopy (AFM); X-ray photoelectron spectroscopy XPS; Raman spectroscopy; Biological properties.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100600

Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran
Provedor de dados: 52
Autores: Nuñez-Gaona,Oscar; Saucedo-Castañeda,Gerardo; Alatorre-Rosas,Raquel; Loera,Octavio.
The aim of the present work was to study the effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran (WB).The highest growth rate of B. bassiana 885.2 on WB media was obtained at a w=1.0, with no detected growth at a w &lt; 0.97. Solid-state fermentation (SSF) using WB (66% moisture; a w=1.0) achieved a maximal yield of 1.18x10 10 conidia per gram of dry substrate (gds). This yield decreased one order of magnitude with higher moisture contents or the addition of sugarcane bagasse (SCB) as a texturiser. In SSF using WB (66% humidity),the time to obtain a yield of 1x10(10) conidia/gds, referred to as t10, could be predicted using a model considering common inoculum levels and maximal yields. For...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beauveria bassiana; Wheat bran; Water activity; Texturiser; Conidial yields.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400004

Hemocyte quantitative changes in Anticarsia gemmatalis (Lepidoptera: Noctuidae) larvae infected by AgMNPV
Provedor de dados: 52
Autores: Andrade,Fábio Goulart de; Negreiro,Maria Cláudia Cordeiro de; Levy,Sheila Michele; Fonseca,Inês Cristina de Batista; Moscardi,Flávio; Falleiros,Ângela Maria Ferreira.
The initial effects of the infection by AgMNPV in the total and differential counts of the hemocytes in Anticarsia gemmatalis (Lepidoptera: Noctuidae) larvae were studied. The total number of the hemocytes did not decrease in infected larvae, as it occurred in non infected larvae. In infected larvae, the hemocyte types showed the following frequencies: plasmatocytes - 47.8%, esferulocytes - 25.9%, granulocytes - 15.8%, oenocytoids - 7.2%, prohemocytes - 2.8%, vermicytes - 0,5%. Only the percentage of the granulocytes was different among infected and non infected larvae, indicating that these cells responded quickly to the initial viral infection. These results showed the effective role of the hemocytes in the response of the A. gemmatalis to the infection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Velvetbean caterpillar; Viral infection; Cellular defense; DHC; THC.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200005

Discrimination between workers of Acromyrmex subterraneus molestans from monogynous and polygynous colonies
Provedor de dados: 52
Autores: Souza,Danival José de; Lucia,Terezinha Maria Castro Della; Barbosa,Luiz Cláudio de Almeida.
Behavioral assays were conducted with individuals from monogynous and polygynous colonies of Acromyrmex subterraneus molestans to evaluate the discriminatory ability of ant workers. These bioassays showed that this subspecies could not discriminate among non-nestmates or nestmate workers. However, nestmates of these same colonies did discriminate among workers of another subspecies Ac. subterraneus subterraneus. When discrimation occurred there were no differences in the response of workers from either monogynous or polygynous colonies. Similarities or differences in the chemical profile of both subspecies explained the absence or occurence of aggressiveness among workers. The chemical profile of colonies of the same subspecies was very similar among them,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf-cutting ants; Nestmate discrimination; Polygyny.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300013

Biochemical characterization of selected plant species from Brazilian Savannas
Provedor de dados: 52
Autores: Caramori,Samantha Salomão; Lima,Claudinei Sousa; Fernandes,Kátia Flávia.
The aim of this work was to analyze and quantify the presence of antinutritional compounds such as lectins and trypsin-like inhibitors, polyphenols and tannins, and enzymatic activity of peroxidases and proteases in the seeds of Annona crassiflora Mart. (araticum), Hymenaea courbaril L. var. courbaril (jatobá), Plathymenia reticulata Benth. (vinhático), Zanthoxylum rhoifolium Lam. (maminha de porca), Apeiba tibourbou Aubl. (pau jangada), Salacia crassiflora Mart G. Don. (bacupari), and Sclerolobium paniculatum Vog. (carvoeiro). The results suggested that these plants could be used as new source of food.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado plants; Plant enzymes; Biochemical characterization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200013

Use of Inert Gases for the Preservation of Nuclear Blood Cells
Provedor de dados: 52
Autores: Khudyakov,Andrey Nikolaevich; Polezhaeva,Tatyana Vitalyevna; Zaitseva,Oksana Olegovna; Sergushkina,Marta Igorevna; Solomina,Olga Nurzadinovna.
Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clathrates; Cryopreservation; Xenon; Krypton; Leukocytes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100404

Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers
Provedor de dados: 52
Autores: Oliveira,Luciana do Valle Rego; Faria,Ricardo Tadeu de; Ruas,Claudete de Fátima; Ruas,Paulo Maurício; Santos,Melissa de Oliveira; Carvalho,Valdemar P.
In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catasetum; Molecular marker; Orchid; RAPD; Genetic similarity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200017

Bioconversion of biomass: a case study of ligno-cellulosics bioconversions in solid state fermentation
Provedor de dados: 52
Autores: Pandey,Ashok; Soccol,Carlos R.
Lignocellulosic residues obtained from crops cultivation form useful sources to be used as substrate for bioconversion processes. Sugarcane bagasse, which is a complex substrate obtained from the processing of sugar cane, is an important biomass among such sources. Due to its abundant availability, it can serve as an ideal substrate for microbial processes for the production of value added products. This paper reviews recent developments on biological processes developed on production of various products in solid state fermentation using sugarcane bagasse as the substrate and describes production of protein enriched feed, enzymes, amino acid, organic acids and compounds of pharmaceutical importance, etc. through microbial means.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ligno-cellulosics; Sugarcane bagasse; Bioconversion; Solid state fermentation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400001

Multipotent male germline stem cells (mGSCs) from neonate porcine testis
Provedor de dados: 52
Autores: Niu,Zhiwei; Wu,Siyu; Wu,Chongyang; Li,Na; Zhu,Haijing; Liu,Weishuai; Hua,Jinlian.
ABSTRACT Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, during which unlimited spermatozoa is produced daily derived from SSCs in the testis throughout life of the male. Germline stem (GS) cells can be isolated from spermatogonia, which shared the characteristics of SSCs and embryonic stem cells (ESCs), and can be passaged stably in vitro. The study of GS cells contributes to understanding spermatogenesis process. However, little is known about the GS cells in domestic animals. Here, we report the successful establishment of a serum- and feeder-free system for multipotent male GS cells (mGSCs) from postnatal porcine testis. These cells expressed pluripotent markers, such as Oct4, Nanog, C-myc, and germline-specific markers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogonial stem cells (SSCs); Multipotent male GS cells (mGSCs); Spermatid-like cells; Porcine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100402

Physiological and cellular responses in two populations of the mussel Perna perna collected at different sites from the coast of São Paulo, Brazil
Provedor de dados: 52
Autores: Abessa,Denis Moledo de Souza; Zaroni,Letícia Pires; Sousa,Eduinetty Ceci Pereira Moreira de; Gasparro,Marcia Regina; Pereira,Camilo Dias Seabra; Rachid,Bauer Rodarte de Figueredo; Depledge,Michael; King,Rebecca Susan.
The physiological conditions of mussels from Ubatuba and Santos and also of organisms transplanted from Ubatuba to Santos were studied by using different techniques. Assays for lysosomal stability were conducted on the haemolymph. Heart rate activity was monitored for 6h. The embryonic development of larvae obtained from the collected mussels was analysed. For all the compared groups of mussels, no significant differences were observed for the cardiac activity monitoring and the embryonic bioassays. The mean Neutral Red (NR) retention time was similar for the animals from Santos and Ubatuba, whereas the organisms transplanted to Santos showed a reduction in the retention time of the dye, indicating damage in the lysosomal membranes. These differences were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Perna perna; Neutral red retention assay; Capmon system; Embryonic assay; Biomarkers; Physiology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200008

Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation
Provedor de dados: 52
Autores: Mustafa,Siti Ratina; Husaini,Ahmad; Hipolito,Cirilo Nolasco; Hussain,Hasnain; Suhaili,Nurshikin; Roslan,Hairul Azman.
Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH9 employing response surface methodology (RSM).Central composite design (CCD) was applied to determine the optimal fermentation condition with respect to the four main process parameters such as temperature, initial moisture content, pH and the incubation period. Solid state fermentation (SSF) was performed using 5.0 g of sago hampas inoculated with 1x107sporesmL-1following the experimental design obtained using CCD and further optimized by RSM. The initial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid state fermentation; Aspergillus flavusNSH9; Sago hampas; Central Composite Design; Response Surface Methodology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100416

Use of RAPD molecular markers on differentiation of brazilian and chinese Ganoderma lucidum strains
Provedor de dados: 52
Autores: Rolim,Leonardo do Nascimento; Cavalcante,Maria Auxiliadora de Queiroz; Urben,Arailde Fontes; Buso,Glaucia Salles Cortopassi.
The aim of this work was to analyze the Brazilian and Chinese strains of Ganoderma lucidum with molecular RAPD markers. A similarity matrix was elaborated and the RAPD profiles of G. lucidum strains were also compared to two other Ganoderma spp: G. applanatum and G. lipsiense in order to produce genetic similarity among the species. Based on the primers used, it was possible to determine that the Brazilian strains and Chinese strain CC-22 are alike. The method and the primers selection showed to be appropriate for the genetic identification of G. lucidum strains, enabling them to be improved and used in research, as well as in the world market.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganoderma lucidum; Genetic diversity; RAPD.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200008

Protective Effects of Edaravone against Methamphetamine-Induced cardiotoxicity
Provedor de dados: 52
Autores: Koohsari,Motahareh; Shaki,Fatemeh; Jahani,Daniel.
ABSTRACT Methamphetamine (METH) is widely abused in worldwide. METH use could damage the dopaminergic system and induce cardiotoxicity via oxidative stress and mitochondrial dysfunction. Edaravone, a sedative-hypnotic agent, is known for it's antioxidant properties. In this study we used edaravone for attenuating of METH-induced cardiotoxicity in rats. The groups (six rats in each group) were as follows: control, METH (5 mg/kg IP) and edaravone (5, 10 and 20 mg/kg, IP) was administered 30 min before METH. After 24 hours, animals were killed, heart tissue was separated and mitochondrial fraction was isolated and oxidative stress markers were measured. Edaravone significantly (p&lt;0.05) protected the heart against lipid peroxidation by inhibition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edaravone; Methamphetamine; Cardiotoxicity; Oxidative stress; Mitochondria.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100342

Quinoa and flaxseed: potential ingredients in the production of bread with functional quality
Provedor de dados: 52
Autores: Calderelli,Valéria Alcântara Santos; Benassi,Marta de Toledo; Visentainer,Jesuí Vergílio; Matioli,Graciette.
The objective of this work was to compare the physicochemical characteristics of quinoa and flaxseed bread. Sensory acceptance, color and texture were also appraised. They showed appropriate balances between their content of polyunsaturated and saturated fatty acids and low levels of trans fatty acids. Flaxseed bread had larger amounts of mono and polyunsaturated fatty acids, omega-6 and omega-3, as well as a more balanced omega-6/omega-3 ratio. Quinoa bread, on the other hand, had the advantage of presenting smaller contents of saturated fatty acids. With regard to color and texture, quinoa bread had similar characteristics to the flaxseed bread. The quinoa bread was well accepted by the consumers, who expressed high interest in buying it.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Chenopodium quinoa; Linum usitatissimum; Functional food.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400029

Enhanced Production of Vitamin K2 from Bacillus subtilis (natto)by Mutation and Optimization of the Fermentation Medium
Provedor de dados: 52
Autores: Song,Junying; Liu,Hongxia; Wang,Li; Dai,Jun; Liu,Yan; Liu,Hui; Zhao,Genhai; Wang,Peng; Zheng,Zhiming.
The aim of this study was to enhance the production of vitamin K2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG) and low energy ion beam implantation and optimizing the fermentation medium. Mutation resulted in 1.66-fold higher production of vitamin K2 than that of the parentl strain. The production by the mutant BN-P15-11-1was increased 55% and reached 3.593±0.107 mg/L by using the Plackett-Burman and Box-Behnken designs to optimize the fermentation medium. The optimal fermentation culture medium was composed of (g/L) glycerol 69.6, sucrose 34.5, K2HPO4 4.0, peptone 20, yeast extract 25 and fermented at 37 °C and 150 rpm for 72 h. The results showed that the NTG and low energy ion beam implantation mutations and optimizing fermentation medium were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus subtilis (natto); Mutant; Optimization; Vitamin K2.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400606

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design
Provedor de dados: 52
Autores: Antelo,Francine Silva; Costa,Jorge Alberto Vieira; Kalil,Susana Juliano.
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG 1500 or 4% (w/w) PEG 8000 (g/gmol) and 23 or 22.5% (w/w) of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-phycocyanin; Purification Factor; Protein; Polyethylene glycol.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100001

Production and characterization of endo-polygalacturonase from Aspergillus niger in solid-state fermentation in double-surface bioreactor
Provedor de dados: 52
Autores: Hendges,Diogo Henrique; Montanari,Queli; Malvessi,Eloane; Silveira,Mauricio Moura da.
Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of height was evaluated in a cylindrical double surface bioreactor in 96-h experiments. Cell concentration close to 92 mg.g -¹ dm (mg per g of dry medium) in the standard condition (static) was achieved, whereas in tests under forced aeration of 1.4 and 2.8 L.min-1. Kg-1 mm (L of air per minute per Kg of moist medium) and with the central shaft fungal biomass attained approximately 100 mg.g-1 dm. Superior endo-PG activity was obtained with the central-shaft system, 78 U.g-1 dm (units per g of dry medium). Forced aeration and pressure pulse showed no positive effect on the production of endo-PG, 45 U.g-1 dm and 28 U.g-1 dm, respectively. None of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Double-surface bioreactor; Endo-polygalacturonase; Solid-state fermentation; Enzyme stability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200005

Effects of hydrolysis and digestion in vitro on the activity of bovine plasma hydrolysates as inhibitors of the angiotensin I converting enzyme
Provedor de dados: 52
Autores: Gómez Sampedro,Leidy Johanna; Zapata Montoya,José Edgar.
The angiotensin I-converting enzyme (ACE) inhibiting activity of bovine plasma hydrolyzates obtained by Alcalase 2.4 L at different degrees of hydrolysis (DH) was evaluated. For the evaluation of ACE inhibition (ACEI), Hippuryl-His-Leu was used as substrate and the amount of hippuric acid liberated by non-inhibiting ACE was determined by spectrophotometry at 228 nm. The results showed that the enzymatic hydrolysis increased the ACEI activity as compared with the un-hydrolyzed plasma. The highest activity was onbtained with a DH of 6.7%. The peptide fractions with the maximum activity were isolated using ultrafiltration membranes, ion exchange chromatography and high performance liquid chromatography on reverse phase (RP-HPLC). The fraction with highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic hydrolysis; Bioactive peptides; Angiotensin I-converting enzyme inhibitors; Peptide sequence.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300012

Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp
Provedor de dados: 52
Autores: Lopes,Francisco Luiz Gumes; Severo Júnior,João Baptista; Souza,Roberto Rodrigues de; Ehrhardt,Daniela Diniz; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; Bromelain; Membrane separation processes; Purification; Medicinal product.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024

Some Biochemical properties of polyphenoloxidase from spearmint (Mentha arvensis)
Provedor de dados: 52
Autores: Neves,Valdir Augusto; Picchi,Douglas Gatte; Silva,Maraiza Aparecida da.
Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity was lost after 60 and 15 sec at 70 and 75ºC, respectively. Measuring of residual activity showed a stabilizing effect of sucrose at various temperatures with activation energy (Ea) for inactivation increasing with sucrose concentration from 0 to 40% (w/w). Ea values of 78.13; 80.37; 82.79 and 81.00 kJ/Mol were found for 0, 15; 30 and 40% sucrose, respectively. PPO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyphenoloxidase; Mentha arvensis; Characterization; Heat inactivation; Inhibitors.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400025

Performance and macrophage activity of broilers fed with a sorghum meal with different yeast wall levels
Provedor de dados: 52
Autores: Ferreira,Suelen Regina; Murakami,Alice Eiko; Silveira,Thais Gomes Verzignassi; Santos,José Maurício Gonçalves dos; Fernandes,Jovanir Inês Muller.
The aim of this work was to evaluate the effect of broiler breeders' age and yeast wall (YW) levels on broilers' performance and macrophage activitys. The experiment consisted in a completely randomized design and a 2 X 5 factorial arrangement and two controls of two broiler breeders age (34 and 57 weeks of age) and five YW levels (zero, one, two, three or four kg of YW/ton of diet). They received sorghum diet compared to a control corn/soybean meal diet. The age of the broiler breeders influenced the performance, but did not affect macrophage activity. At 34 weeks age broiler breeders' progeny, all diets were similar considering the average weight. For 57 weeks age broiler breeders' progeny, at 21 days, only average weight of sorghum diet supplemented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcass yield; Mananoligossacarydes; Phagocytosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200019

Saccharomyces uvarum mannoproteins stimulate a humoral immune response in mice
Provedor de dados: 52
Autores: Darpossolo,Fernanda Patrícia Brito; Eto,Silas Fernandes; Venancio,Emerson José; Castro-Goméz,Raul Jorge Hernan.
Yeasts discarded in industrial processes can be used as a nutritional supplement and to extract cellular components with biotechnological aims. In this study, the humoral immune response of Swiss mice treated with mannoproteins (MP) from the yeast Saccharomyces uvarum was evaluated. The mice were treated with MPs at different doses and times and inoculated with 2% sheep red blood cells. An increase in total Ig in mice treated with 100 μg of MP at the time of immunization or 24 h before was observed in the primary immune response; in the secondary immune response, an increase was observed in total Ig values for all groups, and an increase of IgG was observed in the mice treated with MPs (100 μg) at the time of immunization or 24 h before. These results show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycoprotein; Swiss mice; Antibody production; Yeast; Cell wall.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400016

Application of an Enzymatic Extract from Aspergillus niger as Coagulant for Cheddar Cheese Manufacture
Provedor de dados: 52
Autores: Lombardi,Julia; Ciocia,Felicia; Uniacke-Lowe,Thérèse; Boeris,Valeria; Risso,Patricia; McSweeney,Paul L. H.
Abstract The coagulation of milk by a serin protease from Aspergillus niger NRRL3 was studied by rheology. Cheddar-type cheese was manufactured using 3.5% (v/v) of fungal enzymatic extract and fermentation-produced chymosin was used as control coagulant. Full composition and ripening of both kinds of Cheddar cheese were studied. Differences in the proteolysis of caseins, not only during cheese manufacture but also during ripening, affected cheese composition, texture and peptide profile. Microbial development during ripening was not affected by the coagulant used.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial coagulant; Cheese; Biochemistry during ripening.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100509

A Mathematical Model for the Removal of Organic Mater in Stabilization Ponds
Provedor de dados: 52
Autores: Medri,Waldir; Medri,Vandir.
This work presents an application to systemize the construction of ponds systems for treatment of domestic sewage. It consisted of two anaerobic ponds operated in parallel during May/97 to April/99. These were connected in series with a chicaned facultative pond. The treatment system was controlled with samples collected from the crude sewage (compound sample), in the affluents and effluents of the ponds and along the flux of the anaerobic and facultative ponds. The following parameters were analyzed: pH, Biochemical Oxygen Demand, Chemical Oxygen Demand, Total Solids, Sedimentable Solids, Total Coliforms, Oxygen Consumed in Acid Medium (OCAM) and temperature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stabilization ponds; Mathematical model; Optimization.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100014

Physico-chemical and sensorial evaluation of sugarcane spirits produced using distillation residue
Provedor de dados: 52
Autores: Menezes,Evandro Galvão Tavares; Alves,José Guilherme Lembi Ferreira; Valeriano,Carolina; Guimarães,Isabela Costa.
The objective of the present study was to analyze the use of vinasse from cachaça as an ingredient of the fermentation medium for the spirit production. The fermentations were conducted out in three successive batches using a Saccharomyces cerevisiae isolate. In the first batch, the sugarcane broth was only diluted with distilled water. In the second and third batches, the fermentations were carried out using the cane broth diluted with vinasse from the distillation of the sugarcane wines of the first and second batches, respectively at a concentration of 10% (v/v). The spirits were submitted to the physicochemical and sensorial analyses. The results showed that vinasse addition did not affect the fermentation, distillation and chemical-sensorial quality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vinasse; Ethanol; Sugarcane spirits; Physico-chemical composition; Sensory analysis; Saccharomy cescerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100016

Phenolic compounds and antioxidant activity of rice
Provedor de dados: 52
Autores: Walter,Melissa; Marchesan,Enio.
The aim of this work was to study the phenolic compounds identified in rice, their antioxidant activity and their potential beneficial effects on health. In vitro and in vivo studies evaluating the rice grains with different pericarp colour (light brown, red and black) showed potential beneficial effects on health related to the polyphenol content of the grain, such as reduction of oxidative stress, aid in the prevention of cancer, cardiovascular diseases and complications of diabetes, among others.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice; Red rice; Black rice; Polyphenols; Antioxidant; Biological effect.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200020

Fetal mummification in silky shark (Carcharhinus falciformis) from the Gulf of California, Mexico
Provedor de dados: 52
Autores: Sandoval-Castillo,Jonathan; Villavicencio-Garayzar,Carlos.
The aim of this work was the description of fetal mummification in silky shark. During July of 2001 in Santa Maria fishing camp, Gulf of California Mexico, fourteen pregnant female of silky sharks were captured. In all of them the embryos were found to be dehydrated. However, total observations were possible in only two litters. Six embryos in each analyzed litters were found. All the embryos were mummified, complete and without maceration. Because the litters were totally mummificated in several females, it was suggested that fetal mummification was produced by the environmental condition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fetal mummification; Carcharhinus falciformis; Carcharinidae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300015

Development and growth curve of the pine cones of Araucaria angustifolia (Bert.) O. Ktze, in the region of Curitiba - PR
Provedor de dados: 52
Autores: Anselmini,Justina Inês; Zanette,Flávio.
The objectives of this work were to describe the development and growth rate of the pine cones of Araucaria angustifolia during and after the pollination period, to identify the period of maximum growth of the cones and the period of pollination and maturation of the pine nuts. The adult individuals were found at the Setor de Ciencias Agrarias of the UFPR, in Curitiba - PR. Collections and measurements of pine cones were made in 2003 and 2005. The diameter and the length of the pine cones were measured 15 different times in both the years. In the studied environmental conditions, the period between the pollination and maturation of the pine nuts was 20 months. The maximum pine cone growth was achieved between October and January after the pollination, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parana-pine; Strobili; Pollination.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400003

Central venous access through the external jugular vein in children submitted to bone marrow transplantation
Provedor de dados: 52
Autores: Godoy,José Luiz de; Otta,Edson Keity; Miyazaki,Ricardo Atsumori; Bitencourt,Marco Antonio; Pasquini,Ricardo.
Establishment of long-term central venous access is a sine qua non step for bone marrow transplantation in children. Most frequently, long-term central venous access has been obtained via blind percutaneous cannulation of subclavian and internal jugular veins or via internal jugular vein cutdown. In order to avoid some potential minor and major complications associated with the subclavian or internal jugular approaches, the authors describe an easy, simple and safe method for central venous access through an external jugular vein cutdown that should be of interest to readers involved in the field of bone marrow transplantation. It should be also considered for children as well as adults needing central venous access via an external catheter - or totally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central venous access; External jugular vein; Bone marrow transplantation; Catheter.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100007

Global and Local Oriented Gabor Texture Histogram for Person Re-identification
Provedor de dados: 52
Autores: Poongothai,Elango; Suruliandi,Andavar.
ABSTRACT: Automated person re-identification is a key process in global distributed camera systems. This paper proposes a new feature, the Global and Local-Oriented Gabor Texture Histogram (GLOGTH), for person re-identification. GLOGTH is a combination of the local texture and global structure information of a given input image. This feature aims at representing the human appearance traits with low-dimensional feature extraction. The proposed feature extracts the texture information of input images based on the orientation of the weighted gradient from the global representation. In GLOGTH, the principal orientation is determined by the gradient of the pixels. Based on the principal orientation, the Gabor feature is extracted and imbues GLOGTH with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Person Re-identification; Texture Feature; Global feature; Local feature.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100609

Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
Provedor de dados: 52
Autores: Lima,Eleonidas Moura; Lopes,Otávio Sérgio; Soares,Leonardo Ferreira; Arruda,Talitta Dantas; Gigek,Carolina Oliveira; Melo,Cynthia Germoglio Farias; Smith,Marilia Arruda Cardoso; Oliveira,João Ricardo Gonçalves; Medeiros,Arnaldo; Delatorre,Plínio; Burbano,Rommel Rodriguez.
Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P &lt; 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P &gt; 0.05). These results suggest that the SNP rs1695 of the GSTP1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DSASP method; Genotyping; Gastric Cancer.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414

Major soil classes of the metropolitan region of Curitiba (PR), Brazil: I - mineralogical characterization of the sand, silt and clay fractions
Provedor de dados: 52
Autores: Pires,Ana Christina Duarte; Melo,Vander de Freitas; Lima,Valmiqui Costa; Motta,Antônio Carlos Vargas.
The aim of this work was to evaluate the mineralogical and chemical characteristics of most representative soils of the Region of Curitiba, Paraná State. Samples were collected at different depths. The results showed: (a) the quartz was the only identified mineral at the silt and sand fractions. The dominant clay mineral was Kaolinite, with contents ranging from 676.7 to 820.8 g kg-1. The gibbsite was also an important constituent of the most weathered horizons and the hematite and goethite contents were low, mainly in the Histosol; (b) at the C horizon of the Inceptisol, high intensity of vermiculite/smectite reflections were detected (X-ray diffraction), justifying the high capacity of expansion and contraction, normally showed for this soil horizon; (c)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kaolinite; Crystalline and amorphous Fe and Al oxides; 2:1 minerals; XRD.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200001

Molecular Cloning and Sequencing of AlkalophilicCellulosimicrobium cellulans CKMX1 Xylanase Gene Isolated from Mushroom Compost and Characterization of the Gene Product
Provedor de dados: 52
Autores: Walia,Abhishek; Mehta,Preeti; Guleria,Shiwani; Chauhan,Anjali; Shirkot,C.K.
ABSTRACT A xylanolytic bacterium was isolated from mushroom compost by using enrichment technique. Results from the metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16S rDNA sequencing suggested the bacterium to be Cellulosimicrobium cellulans CKMX1. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 1671 bp was successfully amplified using PCR and cloned into Escherichia coli DH5α. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1, 4-beta-xylanase, which was a member of glycoside hydrolase family 11. It showed 98% homology withCellulosimicrobium sp. xylanase gene (Accession no. FJ859907.1) reported...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulase free xylanase; Cellulosimicrobium cellulans; E.coli; Cloning; Gene.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600913

An Alternative Inactivant for Rift Valley Fever Virus using Cobra Venom-derived L-Amino Oxidase, which is Related to its Immune Potential
Provedor de dados: 52
Autores: Al-Olayan,Ebtesam M; Mohamed,Aly F; El-Khadraqy,Manal F; Shebl,Rania I; Yehia,HanryM.
ABSTRACT Vaccine improvement depends on the formulation, adjuvant type and inactivant used. The type of formulation may interfere with immunogenicity. The present work aimed to evaluate the inactivation activity and related immune potential of the Cobra venom-derived LAO enzyme compared to the currently used inactivants (BPL and formalin) for both animal and human vaccines. The RVF virus was completely inactivated within 6 hrs, 4 hrs and 2 hrs after treatment with Formalin, LAO and BPL, respectively. The vaccine potency [ED50] was arranged in a descending order from formalin (0.016) to BPL (0.005) and LAO (0.002). The total IgG levels, Neutralizing Index (NI) and Interferon levels were significantly increased compared to those detected after immunization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rift Valley fever; Inactivant; Humoral immunity; Interferon; Interleukins; LAO.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100345

Properties of a biosurfactant produced by Bacillus pumilus using vinasse and waste frying oil as alternative carbon sources
Provedor de dados: 52
Autores: Oliveira,Juliana Guerra de; Garcia-Cruz,Crispin Humberto.
Biosurfactants are chemical molecules produced by the microorganisms with potential for application in various industrial and environmental sectors. The production parameters and the physicochemical properties of a biosurfactant synthesized by Bacillus pumilus using different concentrations of vinasse and waste frying oil as alternative carbon sources were analyzed. The microorganism was able to grow and produce a biosurfactant using both the residues. The surface tension was reduced up to 45 mN/m and the maximum production of crude biosurfactant was 27.7 and 5.7 g/l for vinasse and waste frying oil, respectively, in concentration of 5%. The critical micelle concentration (CMC) results of 1.5 and 0.2 g/l showed the efficiency of the biosurfactant produced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactants; Vinasse; Waste frying oil; Bacillus pumilus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100020

Effect of formulation on the quality of frozen bread dough
Provedor de dados: 52
Autores: Salas-Mellado,Myriam M.; Chang,Yoon Kil.
The main objective of this work was to determine the influence of formulation on the stability of bread dough during frozen storage. Bread doughs containing gluten and trehalose were submitted to mechanical freezing at -30° C and stored frozen for 45 days. Two types of instant yeast were tested: (A) for sweet doughs and (B) for savoury doughs. Specific volume was significantly affected by the yeast type, type A showing better effect than type B. Frozen storage of the doughs negatively affected the specific volume, crumb hardness and technological score of the bread. The addition of 10% trehalose had a beneficial effect on the cell survival rate for both the yeasts.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breadmaking; Frozen dough; Formulation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300018

Comparisons between continuous and batch processing to produce clavulanic acid by Streptomyces clavuligerus
Provedor de dados: 52
Autores: Baptista-Neto,Álvaro; Teodoro,Juliana Conceição; Cassiano Filho,Luiz Claudio Macedo; Badino,Alberto Colli; Hokka,Carlos Osamu.
The aim of the present work was to compare CA production in continuous culture with and without cell recycling and in batch process by Streptomyces clavuligerus. Continuous cultivations with high cell concentration using cell recycling were performed utilizing a hollow fiber ultrafiltration module to separate cells from the filtrate broth. The continuous cultures without cell recycling and the batch cultivations were performed conventionally. The highest productivity was attained in the continuous cultivation with cell recycling (22.2 mg.L-1.h-1). The highest CA concentration was obtained in the batch process (470 mg.L-1.h-1).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clavulanic acid; Streptomyces clavuligerus; Continuous culture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400012

Study of the Potential of Photovoltaic Microgeneration and Minigeneration from the Grid-Connected Photovoltaic Systems Installed at UTFPR Curitiba Campus
Provedor de dados: 52
Autores: Silveira,Camila de Oliveira; Krasnhak,Larissa Barbosa; Tonolo,Édwin Augusto; Urbanetz Junior,Jair.
Abstract: Humanity is increasingly dependent on energy, which demand grows every year. Renewable energy sources are consolidated alternatives in the market, previously installed on a small scale but now thought as large plants. The correct operation, taking full advantage of the generation potential, depends on studies of the place of implantation, such as radiation levels, temperature, latitude, etc. Two photovoltaic systems installed in the city of Curitiba were studied in order to monitor their respective performances through figures of merit.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic system; Power capacity; Power generation; Figures of merit.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200221

Effect of three different types of culture conditions on Spirulina maxima growth
Provedor de dados: 52
Autores: Monteiro,Maria Paula da Costa; Luchese,Rosa Helena; Absher,Theresinha Monteiro.
Growth of Spirulina maxima was studied in three types of culture conditions with four replicates each: 1) manual aeration with natural sunlight; 2) manual aeration with artificial light; and 3) constant aeration with an aquarium compressor and artificial light. After 185 days of incubation, growth declined in the first two treatments, while in the third treatment, higher growth was observed with average optical density of 3.7 against 1.8 and 1.9 in the first and second treatment, respectively. This was probably due to the fact that under constant aeration the salts were suspended avoiding the crystallization what could cause a decrease in the availability of the necessary nutrients for the growth. Also, the constant stirring allowed all the cells to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquarium compressor; Biomass production; Microalgal growth; Spirulina.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200016

Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
Provedor de dados: 52
Autores: Cortez-Herrera,Elizabeth; Sperhacke,Rosa Dea; Becker,Daniela; Kritski,Afrânio; Zaha,Arnaldo; Rossetti,Maria Lucia Rosa.
The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase Chain Reaction; Tuberculosis; Internal control; Nucleic acid amplification; Uracyl DNA glycosilase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006

Effects of clonal integration on the proximal and distal ramets of Cynodon dactylon under shade stress
Provedor de dados: 52
Autores: Shi,Tao Ying; Quan,Qiu-Mei; Li,Yun-Xiang.
ABSTRACT In a pot experiment, clonal ramets of Cynodon dactylon, a stolon herbaceous plant, were treated with heterogeneous lighting. Proximal ramets (elder ramets) were subjected to shade stress at three different degrees, and stolons between proximal and distal ramets of each pair were treated in a connected or severed manner. Results showed that in moderate shade stress, the number of ramets and leaves, biomass, net photosynthetic rate (Pn), maximum quantum yield (Fv/Fm), effective quantum yield of PSII (ФPSII), and soil and plant analysis development values (SPAD) of proximal ramets were significantly reduced, regardless of whether stolons were kept intact or severed. However, the growth of distal ramets was not significantly influenced, and keeping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clonal integration; Shade stress; Cynodon dactylon; Proximal ramets.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100405

Preparation of pellets containing highly soluble drug by extrusion/spheronisation and coating with Kollicoat® SR 30D
Provedor de dados: 52
Autores: Andreazza,Itamar Francisco; Ferraz,Humberto Gomes.
The aim of this study was to prepare and evaluate the pellets, containing a highly soluble drug (ascorbic acid), by the extrusion-spheronization process and coated with a release controlling polymer. The coating (undertaken in a fluid bed) was applied to three batches of the pellets with a dispersion of Kollicoat® SR 30 D, with each batch of pellets receiving a different level of polymer (5.07; 8.26 and 10.35%). The coated pellets were evaluated for sphericity by imaging analysis and comparative dissolution profile with a product commercially available in Brazil. All of the evaluated samples presented adequate physical properties and the dissolution profile of those coated with 5.07% of polymer proved to be similar to that of the commercially available...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coating; Pellets; Extrusion spheronization; Controlled release.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200013

A Histological Study on Venom Apparatus of Montivipera xanthina (Gray, 1849) (Serpentes, Viperidae)
Provedor de dados: 52
Autores: Kılıç,Dilara Çolak; Akat,Esra; Arıkan,Hüseyin.
ABSTRACT This is the first report describing morphological and histological structure of venom apparatus in Montivipera xanthina. The venom gland apparatus of M. xanthina was composed of four distinct parts, main venom gland, primary duct, accessory gland and secondary duct. The main venom gland was composed of acini. The accessory gland was formed by long and ramified tubules. Nine fangs were detected in one half of the upper jaw each at different stage of development. Only one tooth was functional and the eight remaining fangs form a replacement series. The venom apparatus of viperid snakes are the most effective venom-delivery system among vertebrates and have been the focus of scientific interests for many years. Despite this interest, there are few...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Montivipera xanthina; Venom gland; Fang; Dentine; Odontoblast.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100425

Stability of unpasteurized and refrigerated orange juice
Provedor de dados: 52
Autores: Souza,Maria Cristina Corrêa de; Benassi,Marta de Toledo; Meneghel,Renata Fraxino de Almeida; Silva,Rui Sérgio dos Santos Ferreira da.
The stability of orange juice obtained from a small extractor and stored in a polyethylene bottle was assessed under isothermal and non-isothermal storage conditions at 4, 8 and 12ºC for 72 hours. pH, titratable acidity and Brix did not alter significantly during the 72 hours storage. Microbiological analysis showed high initial count for moulds and yeasts that increased in the juice stored for 72h under the non-isothermal conditions with temperature abuse (12ºC/4h). Date of the sensory evaluation showed a small reduction in product acceptance in this condition. The juice, in the recommended validity period (48h), presented losses of less than 20% of the initial ascorbic acid content regardless of the treatment. However, after this time, the degradation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus fruit; Shelf life; Vitamin C; HPLC; Sensory quality; Moulds and yeasts.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300009

Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil
Provedor de dados: 52
Autores: Prasad,Roopa; Abraham,Theruvath Koshy; Nair,Ananthakrishnan Jayakumaran.
Studies were conducted on the production of protease by moderately halophilic Bacillus sp. on agro-industrial waste materials. The bacterium could efficiently use many agro wastes as substrates but wheat bran supported maximum enzyme production. To ascertain the performance of the process in shake flasks and lab scale bioreactor, experiments were conducted to analyse protease activity utilizing wheat bran as cost effective substrate. The studies unveiled that pH 7.0, temperature 30°C and static conditions were optimal for enzyme production in flask level fermentation. In scale-up fermentation, at optimal pH and temperature, agitation rate of 50 rpm was best for protease production. The enzymatic nature was studied in 10% SDS gels with BSA (2.5 mg/mL) as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poultry soil; Endoprotease; Scale up fermentation; Wheat bran; Halotolerant.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300020

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.
Provedor de dados: 52
Autores: Machado,Marília Pereira; Silva,André Luís Lopes da; Biasi,Luiz Antonio; Deschamps,Cícero; Bespalhok Filho,João Carlos; Zanette,Flávio.
In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: True lavender Micropropagation CaCl2.2H2O; Leaf anatomy Ultrastructure Trichome.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500636

Characterization of an acidification and equalization tank (AET) operating as a primary treatment of swine liquid effluent
Provedor de dados: 52
Autores: Motteran,Fabrício; Pereira,Erlon Lopes; Campos,Claudio Milton Montenegro.
This work evaluated the potential of the acidification equalization tank (AET) used as a primary treatment unit, treating the hog farming wastewater. The treatment system consisted of a degritter with a triangular-notch weir, for measuring the flow, a static sieve, and an acidification and equalization tank (AET), an anaerobic baffled reactor (ABR), an upflow anaerobic sludge blanket (UASB) reactor, a settling tank, a greenhouse for fertirrigation and two infiltration ponds. The AET had a net capacity of 8,000 liters, internally covered with asphalt blanket, worked based on surface loading rates application. The unit operated continuously, with its flow varying from 0.1 to 10 L s-1. To determine the efficiency, the following parameters were measured: pH;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine effluent; Organic matter removal; Primary treatment; Pollution control; Inexpensive technologies.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300017

Modelling antagonic effect of lactic acid eacteria supernatants on some pathogenic bacteria
Provedor de dados: 52
Autores: Portella,Augustus Caeser Franke; Karp,Susan; Scheidt,Gessiel Newton; Woiciechwski,Adenise Lorenci; Parada,Jose Luis; Soccol,Carlos Ricardo.
This work presents a statistical model of survival analysis for three pathogenic bacterial strains (Escherichia coli, Listeria monocytogenes and Staphylococcus aureus), when treated with neutralized and non-neutralized filtered supernatants broth from cultures of Lactobacillus acidhophilus, Lactobacillus rhamnosus and Lactobacillus sake. Survival analysis is a method employed to determine the period of time from an initial stage up to the occurrence of a particular event of interest, as death or a particular culture growth failure. In order to evaluate the potential efficacy of the ahead mentioned lactic acid bacteria when used as bioprotective starters in foods, experimental data were statistically treated and expressed by simple representative curves....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statistical model; Survival analysis; Bioprotection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700004

Tolerance of Anadenanthera peregrina to Eucalyptus camaldulensis and Eucalyptus grandis essential oil as condition for mixed plantation
Provedor de dados: 52
Autores: Duarte,Neimar de Freitas; Karam,Decio; Bucek,Elizabeth Uber; Muzzi,Maria Rita Scotti.
With the purpose of selecting the species of woody Caatinga for mixed plantations with Eucalyptus spp., the allelophatic effects of E. camaldulensis and E. grandis essential oil were studied on the growth activities of Anadenanthera peregrina. The plants were closed in glass chambers in the presence of volatile oil of E. camaldulensis or E. grandis at the concentration of 13 nl.cm-3. The number of leaves, height and diameter at soil lever were compared before, immediately after and after 30 days. Chlorophyll a and b, carotenoids and dry mass were evaluated after the treatment application. There was no inhibitory effect of E. camaldulensis and E. grandis oils on A. peregrina. E. camaldulensis, which was more adapted to semi-arid conditions, was planted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allelophatic; Caatinga; Angico.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300013

Characterisation of Cassava Bagasse and Composites Prepared by Blending with Low-Density Polyethylene
Provedor de dados: 52
Autores: Farias,Fabiane Oliveira; Jasko,Ariana Crasnhak; Colman,Tiago André Denck; Pinheiro,Luís Antônio; Schnitzler,Egon; Barana,Ana Cláudia; Demiate,Ivo Mottin.
The main objective of this study was to characterise the cassava bagasse and to evaluate its addition in composites. Two cassava bagasse samples were characterised using physicochemical, thermal and microscopic techniques, and by obtaining their spectra in the mid-infrared region and analysing them by using x-ray diffraction. Utilising sorption isotherms, it was possible to establish the acceptable conditions of temperature and relative humidity for the storage of the cassava bagasse. The incorporation of cassava bagasse in a low-density polyethylene (LDP) matrix was positive, increasing the elasticity modulus values from 131.90 for LDP to 186.2 for 70% LDP with 30% SP bagasse. These results were encouraging because cassava bagasse could serve as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta Crantz; Residue; Polymers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600821

Thermodynamic modeling of ternary liquid-liquid systems with forming immiscibility islands
Provedor de dados: 52
Autores: Zuber,Andre; Raimundo,Rodrigo; Mafra,Marcos Rogério; Cardozo Filho,Lúcio; Oliveira,José Vladimir; Corazza,Marcos Lúcio.
The aim of this work was to study the application of a stochastic algorithm to correlate the experimental data of island-type systems in ternary systems. Thermodynamic NRTL and UNIQUAC models were used to evaluate the activity coefficients. Results regarding the application of the stochastic algorithm were in good agreement with those presented in the current literature. However, generalization of the method proposed in the present work remained an intriguing and complex task so as to reach broad conclusions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LLE; Island-type; Stochastic algorithm; NRTL; UNIQUAC.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600020

Preliminary qualitative analysis on mtDNA in Astyanax fasciatus populations Cuvier, 1819 (Teleostei; Characidae) indicate population distinctiveness
Provedor de dados: 52
Autores: Matoso,Daniele Aparecida; Martins,Cesar; Artoni,Roberto Ferreira; Galetti Jr,Pedro Manoel.
A preliminary qualitative analysis of genetic variability status in Astyanax fasciatus (Cuvier, 1819) from upper Tibagi River headwaters and Vila Velha State Park (VVSP) was carried out by enzymatic digestion (RFLP) of D-Loop region from mtDNA. The results showed that Tibagi and VVSP populations were genetically different.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neotropical fish; RFLP; Conservation genetics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300022

Feeding adult of Artemia salina (Crustacea-Branchiopoda) on the dinoflagellate Gyrodinium corsicum (Gymnodiniales) and the Chryptophyta Rhodomonas baltica
Provedor de dados: 52
Autores: Costa,Rauquírio André Albuquerque Marinho da; Koening,Maria Luise; Pereira,Luci Cajueiro Carneiro.
Experiments were carried out on feeding performance and survival rates of adult Artemia salina exposed to no axenic strains of the dinoflagellate Gyrodinium corsicum and of the Chryptophyta Rhodomonas baltica. Filtration rates on R. baltica and G. corsicum varied from 3.35 to 7.14 ml.artemia-1.h-1 and from 2.97 to 15.86 ml.artemia-1.h-1, respectively. The ingestion rates observed for A. salina did not indicate any digestive dysfunction or physiological impairment for organisms fed on G. corsicum and their functional response were similar to those observed for other organisms like copepod fed on different food concentrations. Mortality rates oscillated from 2.5% to 100% when A. salina was fed on R. baltica or G. corsicum, respectively. Highest mortality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding; Artemia salina; Rhodomonas baltica; Gyrodinium corsicum.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500011

Decellularized Bovine Articular Cartilage Matrix Reinforced by Carboxylated-SWCNT for Tissue Engineering Application
Provedor de dados: 52
Autores: Mohammadie,Zari Majidi; Parivar,Kazem; Shahri,Nasser Mahdavi; Fereidoni,Masoud; Hayati-Roodbari,Nasim.
ABSTRACT Nanotubes with their unique properties have diversified mechanical and biological applications. Due to similarity of dimensions with extracellular matrix (ECM) elements, these materials are used in designing scaffolds. In this research, Carboxylated Single-Wall Carbon Nanotubes in optimization of decellularized scaffold of bovine articular cartilage was used. At first, the articular cartilage was decellularized. Then the scaffolds were analyzed in: (i) decellularized scaffolds, and (ii) scaffolds plunged into homogenous suspension of nanotubes in distilled water, were smeared with Carboxylated-SWCNT. The tissue rings derived from the rabbit's ear were assembled with reinforced scaffolds and they were placed in a culture media for 15 days. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Articular cartilage; Carboxylated-SWCNT; Extracellular matrix; Natural scaffold; Tissue engineering.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100607

Content Based Geographic Image Retrieval using Local Vector Pattern
Provedor de dados: 52
Autores: Jenitta,A.; Ravindran,R. Samson.
ABSTRACT Large image archives formed by satellite remote sensing missions are getting an increasing valuable source of information in Geographic Information Systems (GIS). The need for retrieving a required image from a huge image database is increasing significantly for the purpose of analyzing resources in GIS. Content Based Geographic Image Retrieval (CBGIR) in the image processing field is the best solution to meet the requirement. In this work, we used Local Vector Pattern (LVP) to extract fine features present in the geographical image and retrieve the applicable images from a large remote sensing image database. The primary idea of our method is generating micro patterns of LVP by the vectors of each pixel that are constructed by calculating the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geosciences; Geographic Information Systems; Content Based Geographic Image Retrieval; Local Vector Pattern.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100600

Development of modified medium for the enhancement in antifungal activity of P. steckii (MF1 mangrove fungi) against verticillium wilt pathogenic fungi of rose
Provedor de dados: 52
Autores: Sabat,Jyotasanmayee; Gupta,Nibha.
The antifungal activity of Penicillium steckii (MF1) against pathogenic fungi of rose causing verticillium wilt was evaluated under differential nutrient condition. A suitable and modified medium was obtained in which test fungus exhibited enhanced antifungal activity and produced larger inhibition zone against the verticillium fungus. The study carried out to modify the medium components at level of nitrogen, carbon source and their concentration on agar plates. Addition of amino acids, metals and plant growth hormones did not show positive effect but glycerol enhanced the antifungal activity very much.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Penicillium; Nutrient; Rose; Verticillium wilt.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400003

Fermentation capacity of Saccharomyces cerevisiae cultures
Provedor de dados: 52
Autores: Alencar,Elvira Maria Bezerra de; Souza-Motta,Cristina Maria de; Walter,Bruno Souza; Santos,Rejane Maria Pessoa; Marques,Olga Martins; Queiroz,Lusinete Aciole de.
This work aimed at the assessment of fermentative capacity of original diploid, monocellular haploid and recuperated diploid cultures of S. cerevisiae in sterilized sugar-cane wort. Twenty eight cultures were analyzed, four being original diploids (URM-4420, Itaiquara Ferment FIT, Lallemand Ferment FLA and Wild Ferment SEL); 12 monocellular haploids from original ones and 12 recuperated diploids from the monocells. The ethanol percentage ranged from 1.7 to 6.2% and the unfermentable reducing sugars from 0.45 to 0.50g/100mL. The highest ethanol percentages were produced by the monocellular cultures URM-MH3 (4.8%) in 12 h and SEL-MH1 (6.2%) in 24 h, corresponding to the productivity values of 3.15 and 2.03 g.L-1.h-1, respectively. The recuperated diploids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Monocellular; Fermentation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400004

Cytogenetic studies in three species of the genus Oligosarcus
Provedor de dados: 52
Autores: Rubert,Marceléia; Margarido,Vladimir Pavan.
Karyotypic studies were carried out on three species of the genus Oligosarcus: O. paranensis, O. pintoi, and O. longisrostris. All of them showed a diploid number of 50 chromosomes and the same karyotypic formulae (4M + 10SM + 16ST + 20A). Silver nitrate staining revealed single NORs in O. longirostris and multiple NORs in O. paranensis and O. pintoi. Heterochromatin seemed to play an important role in the chromosomal diversification of these species. Based on cytogenetical data, speciation hypotheses within this group were proposed, reinforcing the importance of chromosomal studies for a better understanding of evolution within the genus Oligosarcus, as well as within the family Characidae.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic studies; Oligosarcus; Acestrorhynchinae; Chromosome banding.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100015

Xylanase production by a thermo-tolerant Bacillus species under solid-state and submerged fermentation
Provedor de dados: 52
Autores: Gupta,Uma; Kar,Rita.
Effects of xylose on xylanase production by a thermophilic Bacillus sp showed diverse patterns on corn cob (CC) and wheat bran (WB) as sole carbon sources in solid- state fermentation (SSF) and submerged fermentation (SmF). Supplementation of these media with either mineral salt solution (MSS) or yeast extract peptone (YEP) also exerted variable effects. While under SSF, xylose stimulated xylanase synthesis by 44.01%, on wheat bran supplemented with MSS, it decreased the enzyme activity by 12.89% with YEP supplementation. In SmF, however the enzyme synthesis was stimulated by xylose on supplementation with both MSS and YEP by 41.38% and 27.47%, respectively. On corn cob under SSF, xylose repression was significant both with MSS (26.92%) and YEP (23.90%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylanase production; Thermo-tolerant Bacillus species; Xylose induction and repression; Corn cob; Wheat bran; Solid- state; Submerged fermentation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600007

Life history of Talorchestia deshayesii (Amphipoda, Talitridae) in the Ionian sandy beach (southern Italy)
Provedor de dados: 52
Autores: Prato,Ermelinda; Trono,Annunziata; Biandolino,Francesca.
The population structure and reproductive biology of Talorchestia deshayesii (Audouin, 1826) on an exposed sandy beach in Taranto (Italy) was examined during one year cycle. Monthly surveys were performed from September 2002 to August 2003. Individuals were subdivided into sex, measured and abundance were calculated for each sampling. Abundance varied during the study period, with a marked decrease in winter. T. deshayesii, was found in a relative narrow zone near the sea in winter and summer while in spring and autumn the animals were widespread along the supralittoral. Reproduction occurred from February to November. The minimum average CL at the moment of recovery ranged from 0.22 to 0.28 mm. The sex ratio was female biased. The females and males became...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Talorchestia deshayesii; Biology; Life cycle; Mediterranean; Ionian sea; Sandy beach.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400015

The influence of hydrodynamics on the spatial and temporal variation of phytoplankton pigments in a large, sub-tropical coastal lake (Brazil)
Provedor de dados: 52
Autores: Cardoso,Luciana de Souza; Marques,David da Motta.
This study evaluated the spatial and temporal distribution of phytoplankton pigments in Itapeva Lake and its relationship with hydrodynamic aspects. Regarding spatial distribution, a decreasing N®S gradient was generally observed for the pigments, except in summer. This inversion observed during the summer was influenced by the predominant fetch (N-E). The horizontal heterogeneity was proved (ANOVA) for all seasons of the year, except spring. Spatially in spring, the vertical variance was much more significant (p&lt;0.05) than the horizontal one. The sampling shifts presented a permanent degree of variability among the seasons of the year, showing the existence of a diurnal cycle in the concentration of chlorophyll a. This behavior was related to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pigment; Phytoplankton; Shallow lake; Hydrodynamics; Wind; Bloom.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400013

Effects of the application of sewage sludge and petrochemical residue in maize culture as source of micro-nutrients on soils of Paraná state
Provedor de dados: 52
Autores: Pigozzo,Ana T. Jordão; Gobbi,Marlene A.; Lenzi,Ervim; Luchese,Eduardo B.
An evaluation of the potential of two organic residues as source of micro-nutrients in three soils of Paraná state was studied. Treatments consisted of one dose (38 t.ha-1) of sewage sludge and residue of purified used motor oil both neutralized with CaO+MgO mixture in 3:1 rate. Maize (Zea mays L.) was used as reference plant. After 30 days of seeding, the aerial parts of the plants were cut. They were dried at 70ºC till constant weight and ground. Analysis was undertaken after nitric-perchloric digestion. Micro-nutrients levels in the soils and in the aerial parts of the plants were determined by atomic absorption spectrometry. The results showed a production of the aerial parts of the plants treated with sewage sludge higher than those in other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sewage sludge; Oil residue; Environmental pollution; Micro-nutrients.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200002

Relative growth of the mangrove crab Ucides cordatus (Linnaeus, 1763) (Crustacea, Brachyura, Ocypodidae) at Iguape, São Paulo, Brazil
Provedor de dados: 52
Autores: Pinheiro,Marcelo Antonio Amaro; Hattori,Gustavo Yomar.
A total of 2,130 individuals of Ucides cordatus (1,255 males and 875 females) were captured in a mangrove forest at Iguape, São Paulo, Brazil. For each crab, the following body structures were measured: carapace (width = CW; length = CL; depth = CD), 5th abdominal somite (AW), major chelar propodus (length = PL; width = PW; depth = PD), and 1st and 2nd gonopod pairs (length = GL1 and GL2). The Student "t" and Snedecor "F" tests were used to verify any changes in growth allometric rates during ontogeny. The relationships CLxCW, PLxCW (for both sexes), GL1xCW and GL2xCW (males) and AWxCW (females), showed a better fit by two equations for the juvenile and adult phases (p&lt;0.01). The inflexion point size between regression lines, indicated by each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Relative growth; Brachyura; Ocypodidae; Ucides.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600016

Ionizing radiation effects on food vitamins: a review
Provedor de dados: 52
Autores: Dionísio,Ana Paula; Gomes,Renata Takassugui; Oetterer,Marília.
Ionizing radiation has been widely used in industrial processes, especially in the sterilization of medicals, pharmaceuticals, cosmetic products, and in food processing. Similar to other techniques of food processing, irradiation can induce certain alterations that can modify both the chemical composition and the nutritional value of foods. These changes depend on the food composition, the irradiation dose and factors such as temperature and presence or absence of oxygen in the irradiating environment. The sensitivity of vitamins to radiation is unpredictable and food vitamin losses during the irradiation are often substantial. The aim of this study was to discuss retention or loss of vitamins in several food products submitted to an irradiation process.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irradiation; Vitamins; Foods.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500026

Development of a Standard of Solarimetric Stations SONDA
Provedor de dados: 52
Autores: Danderfer,Rafael de Freitas Gasparelo; Pinto,Camila Scotti; Cremasco,Nicole Polityto; Gruetzmacher,Sarah Beatriz; Leludak,Jorge Assade; Tiepolo,Gerson Máximo; Sodré,Caio Jorge Martins; Bronhara,Renan de Oliveira.
Abstract With the increasing of energy demand in Brazil and the unprecedent popular support for the use of energy sources with low environmental impact, the market for photovoltaic energy has become progressively relevant. Thus, it is of strategic importance to gather information of the solar resources either for a particular location or a specific region. This paper presents all the steps taken in order to design a solarimetric station intended to be used as part of the National Institute for Space Research's(INPE)Environmental Database System (SONDA)network, from the specification of the devices and sensors that will take part in it to the structural dimensioning necessary in order to accommodate them. This high-quality network mainly collects and stores...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solar energy 1; Solarimetric station 2; Applied instrumentation 3.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200214

Interrelationships among egg, larvae and maternal characteristics in Persian sturgeon Acipenser persicus
Provedor de dados: 52
Autores: Nazari,Rajab Mohammad; Ghomi,Mohammad Reza; Aquiloni,Laura.
This study was aimed to observe the correlations among egg, larvae and maternal characteristics of Persian sturgeon. A total of 41 females were used and the variables including fertilization rate, incubation survival rate, larvae survival rate during yolk sac absorption (before feeding), and larvae survival rate after first feeding were determined. As a result, fertilization rate was positively correlated with three variables: incubation survival, larvae survival during yolk sac absorption, and GSI (gonadosomatic index). Also, maternal size (age, weight, total length) did not show a significant relation (P &gt; 0.05) with incubation survival, larvae survival during yolk sac absorption and larvae survival at first feeding stage.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egg; Interrelationship; Larvae; Maternal characteristics; Persian sturgeon.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500012

Synthesis and physicochemical characterization of chemically modified chitosan by succinic anhydride
Provedor de dados: 52
Autores: Mello,Karine Gargioni Pereira Correa de; Bernusso,Leandra de Cássia; Pitombo,Ronaldo Nogueira de Moraes; Polakiewicz,Bronislaw.
The N-succinil-chitosan is a chemically modified derivative of the biopolymer chitosan. The succinic anhydride attached to the free amino groups presented along the chitosan's polymer chain imparts to the molecule different physicochemical properties not exhibited before the modification. These chemical modifications enhance chitosan's solubility in slightly acid, neutral and alkaline media. These properties are related to the long alkyl chains attached to hydrophilic parts. In this case the hydrophilic part of D-glucosamine promotes stronger interactions with the water molecules, and consequently, enhances the solubility of the chitosan polymer. Non-modified free chitosan is soluble only in acidic medium (pH &lt; 5.5). These modifications made possible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-succinil-chitosan; Succinic anhydride; Biopolymer; Solubility.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500017

Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
Provedor de dados: 52
Autores: Joshi,Chetna; Khare,Sunil Kumar.
Regulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Induction; Isoforms; Xylanase; Biobleaching.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003

Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection
Provedor de dados: 52
Autores: Parada,José Luis; Gonçalves,Dicezar; Soccol,Vanete Tomaz; Lima,Marisa; Soccol,Carlos Ricardo.
A study from cows with mastitis was performed and Staphylococcus aureus was the predominant pathogen in 46.4 % among 153 studied strains from 276 milk samples of infected cows. Antibiotic resistance of 71 S. aureus isolates was determined in order to search resistant strains to antibiotics of clinical interest, as well as to determine their degree of multi-resistance. It was found that 60% of the S. aureus strains presented resistance to β-lactams, but none to oxacillin, teicoplamin or vancomycin. On the other hand, with the aim of reducing the use of current antibiotics and their associated resistance, a new formulation was introduced. The antimicrobial compounds (P22-P32), demonstrated to be effective in 55% of the 76 mastitis cases studied. The use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Staphylococcus aureus; Antibiotic resistance; Infection reduction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400009

HRL-Local Infinite Triangular Array Languages
Provedor de dados: 52
Autores: Rajaselvi,V. Devi; Kalyani,T.; Thomas,D.G.
ABSTRACT A new subclass of infinite triangular arrays called hrl-local infinite triangular arrays is introduced. We introduce infinite triangular domino systems to recognize the infinite triangular picture language. Also we introduce strictly domino testable ωω-triangular array languages.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hrl-local infinite triangular arrays; Infinite triangular domino systems; Strictly dommo testable ωω-triangular array languages.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300610

Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi
Provedor de dados: 52
Autores: Faria,Terezinha de Jesus; Ferreira,Rafael Sottero; Yassumoto,Lidiane; Souza,José Roberto Pinto de; Ishikawa,Noemia Kazue; Barbosa,Aneli de Melo.
An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocimum gratissimum; Antifungal activity; Eugenol; Phytopathogenic fungi.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002

Anti-Fusarium moniliforme activity and fumonisin biodegradation by corn and silage microflora
Provedor de dados: 52
Autores: Camilo,Simone B.; Ono,Carlos José; Ueno,Yoshio; Hirooka,Elisa Y.
Studies were carried out to isolate microorganisms from corn and silage screened for their ability to inhibit F. moniliforme growth (strain 113F) in association with fumonisin detoxification. Among 150 isolates four Gram-positive bacilli and one yeast with inhibitory activity were selected. The inhibition zone ranged from 50 to 72.5 mm using cultures, and from 25 to 52.5mm for crude alcoholic extract. The isolates S9, S10, S69 (sporulated bacilli) and SE3071 (yeast) degraded 43, 48, 83 and 57% of the initial FB1 concentration, respectively. The pH increased gradually in the medium during incubation for biodegradation process.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium moniliforme; Fumonisin; Biodegradation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200004

Study of potential impacts of using sewage sludge in the amendment of desert reclaimed soil on wheat and jews mallow plants
Provedor de dados: 52
Autores: Mazen,Ahmed; Faheed,Fayza A; Ahmed,Atef F.
This investigation was conducted to study the impacts of using sewage sludge at different concentrations (0, 10, 25, 50, 75%) in amendment of desert reclaimed soil properties and some physiological aspects in wheat and jews mallow plants. Generally adding sewage sludge to desert soil improved the soil texture, raised the organic matter contents, water holding capacity and lowered pH value. The contents of NPK gradually increased as the ratio of sewage sludge increased. The fresh and dry weights and biosyntheses of pigment contents of the variously treated test plants were increased by increasing the sewage sludge levels in the soil. Also, total carbohydrate and protein contents of sewage sludge-treated test plants were positively affected. With respect to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metals; Protein; Proline; Free amino acids.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400022

Reproductive biology of weakly electric fish Eigenmannia trilineata López and Castello, 1966 (Teleostei, Sternopygidae)
Provedor de dados: 52
Autores: Giora,Júlia; Fialho,Clarice Bernhardt.
This study described the reproductive biology of a population of the weakly electric fish Eigenmannia trilineata from southern Brazil, providing the information on the estimation of reproductive period, fecundity, spawning type, first maturation size, and analysis of gonadal morphology and histology of the species, relating these data to alimentary and abiotic characters. The species showed a relatively long reproductive period, a relative fecundity of 0.27 oocytes per mg of total weight and a parcelled spawning type. First maturation size estimated for the females was 80.5 mm and for the males 63.5 mm of total length. Sex ratio did not differ from 1:1 under a X²test (α= 0.01) during all the sampled months. Sexual dimorphism was related to total length,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproductive biology; Gymnotiformes; Eigenmannia; Abiotic factors; Southern Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300014

Anaerobic biodegradability of dairy wastewater pretreated with porcine pancreas lipase
Provedor de dados: 52
Autores: Mendes,Adriano Aguiar; Pereira,Ernandes Benedito; Furigo Jr.,Agenor; Castro,Heizir Ferreira de.
Lipids-rich wastewater was partial hydrolyzed with porcine pancreas lipase and the efficiency of the enzymatic pretreatment was verified by the comparative biodegradability tests (crude and treated wastewater). Alternatively, simultaneous run was carried out in which hydrolysis and digestion was performed in the same reactor. Wastewater from dairy industries and low cost lipase preparation at two concentrations (0.05 and 0.5% w.v-1) were used. All the samples pretreated with enzyme showed a positive effect on organic matter removal (Chemical Oxygen Demand-COD) and formation of methane. The best results were obtained when hydrolysis and biodegradation were performed simultaneously, attaining high COD and color removal independent of the lipase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradability; Hydrolysis; Lipases; Lipids-rich wastewater and biogas.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600003

Chemical and biological evaluation of rejects from the wood industry
Provedor de dados: 52
Autores: Granato,Daniel; Nunes,Domingos Sávio; Mattos,Patrícia Póvoa de; Rios,Ester de Moura; Glinski,Adeline; Rodrigues,Luciana Collares; Zanusso Júnior,Gerson.
This study aimed chemical characterization and microbiological evaluation of extracts obtained from the waste of woods marketed in Paraná State: Peroba-Rosa (Aspidosperma sp.), Roxinho (Peltogyne sp.), Jatobá(Hymenaea sp.), Curupixá (Micropholis sp.), Itaúba (Mezilaurus sp.), Cedrilho (Erisma sp.) and Imbúia (Licaria sp.), whose botanical identifications were based on anatomical studies. The extracts were prepared with different solvents, analyzed by TLC and UV/VIS techniques, and tested against: Proteus mirabilis ATCC15290, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Enterobacter aerogenes ATCC13048, Micrococcus luteus ATCC9341, Klebsiella pneumoniae ATCC13883, Pseudomonas aeroginosa ATCC27853, Staphylococcus aureus, Streptococcus mutans...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical woods; Microbiological evaluation; Chemical profile of woods.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400029

Lysine requirement of starting barrows from two genetic groups fed on low crude protein diets
Provedor de dados: 52
Autores: Fraga,Alessandro Luís; Moreira,Ivan; Furlan,Antonio Claudio; Bastos,Alexandre Orio; Oliveira,Ricardo Pinto de; Murakami,Alice Eiko.
A trial was carried out to determine the lysine requirement for starting barrows fed on ideal protein concept-based diets. Thirty-two pigs from a commercial crossbred genetic group (CCGG, BW=15.9 ± 1.4 kg) and 32 pigs from a dam line one (DLGG, BW= 14.8 ± 1.0 kg) were used. Pigs were allotted to 4 treatments with diets containing increasing levels of total lysine (0.80, 1.00, 1.20 and 1.40%). Methionine+cystine, threonine and tryptophan were adjusted according to ideal protein profile. Data from performance, plasma urea nitrogen (PUN) and carcass composition were analyzed. CCGG showed higher daily feed intake, daily weight gain, PUN and protein:fat ratio in carcass, while DLGG showed higher fat carcass content and nitrogen retention. Fat content and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcass composition; Crystalline amino acids; Nitrogen excretion; Nutrition; Lean meat.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100007

Effect of harvest period on the quality of storage roots and protein content of the leaves in five cassava cultivars (Manihot esculenta, Crantz)
Provedor de dados: 52
Autores: Sagrilo,Edvaldo; Vidigal Filho,Pedro Soares; Pequeno,Manoel Genildo; Scapim,Carlos Alberto; Vidigal,Maria Celeste Gonçalves; Diniz,Sérgio Paulo Severo de Souza; Modesto,Elisa Cristina; Kvitschal,Marcus Vinícius.
The effect of harvest period on the quality of storage roots and leaves of cassava cultivars was determined in an experiment carried out in a randomized complete block design with four replications in a split plot scheme, with five cultivars in the plots and ten harvest times in the subplots. The IAC 13 cultivar had the highest rate of dry matter accumulation in the storage roots and the Mico cultivar the lowest. The period of least dry matter content in the storage roots occurred later for the Fécula Branca, Mico and IAC 14 cultivars, and the minimum starch content in the storage roots occurred later for the Fécula Branca and Mico cultivars. In general, the IAC 13, IAC 14 and Fécula Branca cultivars had higher dry matter content in the storage roots,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Harvest period; Cultivars; Dry matter content; Starch content; Leaf protein.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200022

Carbon Dioxide Biofixation and Production of Spirulina sp. LEB 18 Biomass with Different Concentrations of NaNO3 and NaCl
Provedor de dados: 52
Autores: Moraes,Luiza; Rosa,Gabriel Martins da; Souza,Michele da Rosa Andrade Zimmermann de; Costa,Jorge Alberto Vieira.
ABSTRACT Microalgae are efficient at using solar energy to turn CO2 and nutrients into biomass containing lipids, proteins, carbohydrates and other compounds that may be used to produce bioproducts for human and animal consumption and pharmaceutical use. The aim of this study was to assess the effect of the NaNO3 and NaCl concentration on the growth kinetics, the biomass composition and the ability to biofix CO2 using the microalga Spirulina sp. LEB 18. The assays were carried out according to a 22 central composite design (CCD) with different concentrations of NaNO3 (1.25, 1.88 and 2.50 g L-1) and NaCl (1.00, 15.0 and 30.0 g L-1). The assays were carried out in 2 L vertical tubular photobioreactors at 30°C, 12 h light/dark and an injection of 12.0% v/v of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CO2; Fixation; Growth kinetics; Microalgae; Nutrients.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100410

Assesment of soil erosion by 137Cs technique in native forests in Londrina City, Parana, Brazil
Provedor de dados: 52
Autores: Andrello,Avacir Casanova; Appoloni,Carlos Roberto; Nascimento Filho,Virgílio Franco do.
The aim of this work was to assess the soil erosion process in native forest by the 137Cs methodology. The mass balance model was applied to assess the rates of soil loss in three native forests around of Londrina city, Paraná, Brazil. 137Cs distribution depth was of exponential type for the three forests and 137Cs inventory was 241 Bq m-2 for Mata 1, 338 Bq m-2 for Mata 2 and 325 Bq m-2 for Mata UEL. The soil loss value calculated for three native forests was: 6,684 kg ha-1 yr-1 for Mata 1, 1,788 kg ha-1 yr-1 for Mata 2 and 4,524 kg ha-1 yr-1 for Mata UEL.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cesium-137; Mass balance model; Native forest.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700016

The Evolving Role of Erythropoietin in Lung of Irradiated Rats
Provedor de dados: 52
Autores: Ashry,Omaima Mohamed; Hasan,Hesham Farouk.
ABSTRACT The aim of the present study is to evaluate the possible curative effect of erythropoietin (EPO) on radiation-induced damage to the lung and its renin angiotensin system. EPO (200 U/100g) was i.p. injected to male rats one hour post 6 Gy whole body gamma irradiation. The animals were sacrificed after 14 days post irradiation. Irradiation induced significant drop of haematological values, bone marrow (BM) count, lung oxidative stress markers, glutathione (GSH) and superoxide dismutase(SOD) associated with significant elevation of malondialdehyde (MDA), advanced oxidation protein product (AOPP) and nitric oxide (NO) besides serum inflammatory markers, tumor necrosis factor alpha (TNF-α) and lactate dehydrogenase (LDH). Also serum and lung renin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythropoietin; Radiation; Inflammation; Oxidative stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100312

Evaluation of the water quality of tidal creeks of Pontal do Paraná, Paraná, Brazil
Provedor de dados: 52
Autores: Siqueira,Adriana; Godinho,Mirna Januária Leal; Kolm,Hedda Elisabeth; Machado,Eunice da Costa.
The aim of this wat was to evaluate the water quality in four tidal creeks of Paraná, Brazil, with different anthropic influences. Samples were collected every three months. Temperature, salinity, pH, alkalinity, total CO2, dissolved O2, nitrite, nitrate, N-ammoniacal, phosphate, silicate, particulate suspended matter, humic matter, total chlorophyll, total and fecal coliform bacteria of surface water were measured in each sample and compared. For all the stations water temperature was lower in winter, but ranged within typically subtropical values. Salinity (7 - 25) and pH (7.08 - 7.91) were higher in Perequê tidal creek indicating that only this tidal creek was strongly influenced by the nearby ocean waters. Lower dissolved O2, reaching anoxic levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tidal creek; Eutrophication; Coast of Paraná.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200028

Molecular Characterization of a MYB Protein from Oryza sativa for its Role in Abiotic Stress Tolerance
Provedor de dados: 52
Autores: Deeba,Farah; Sultana,Tasawar; Javaid,Bushra; Mahmood,Tariq; Naqvi,S. M. S.
ABSTRACT The MYB family represents one of the most abundant classes of transcriptional regulators that perform pivotal role under different developmental processes and abiotic stresses. In present study, a MYB gene from Oryza sativa was selected for functional characterization. Bioinformatics analysis revealed that OsMYB1 cDNA encodes R2-R3 type DNA binding domain consisting of 413 amino acids having size of 44 kDa and pI of 6.24. DNA binding domain containing region was cloned and over-expressed in E. coli. Then, the survival of pGEX-OsMYB1 transformed E. coli cells was compared with control plasmid under different concentrations of NaCl, mannitol, high and low temperature. pGEX-OsMYB1 enhanced the survival of cells at high temperature and salinity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; E. coli; Oryza sativa; OsMYB1; PGEX; Real time qPCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100411

Reproductive behavior of the mangrove crab Ucides cordatus (Crustacea; Brachyura; Ucididae)
Provedor de dados: 52
Autores: Linhares,Jussiara Candeira Spíndola; Silva,José Roberto Feitosa.
The aim of the present work was to study the reproductive behavior of the mangrove crab (Ucides cordatus) in the estuary of the Igaraçú River (Piauí, Brazil). Courtship and mating were observed in December 2007 and in January-March 2009. The most frequently observed reproductive behavior was the transference of eggs onto the abdomen of the female. Some disputes between males and some copulations were observed. The males mated with more than one female each night and all mating activity occurred at night. The establishment of a closed season coinciding with the mating season represented an effective strategy for the preservation of the species when the socioeconomic situation of the community of gatherers was taken into account.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Courtship; Mating; Maturation; Estuary; "Andada"; Seasonality.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600014

Clinostomum complanatum (Digenea, Clinostomidae) density in Rhamdia quelen (Siluriformes, Pimelodidae) from South Brazil
Provedor de dados: 52
Autores: Vianna,Rogério Tubino; Pereira Júnior,Joaber; Brandão,Deodoro Atlante.
The density of Clinostomum complanatum metacercariae by the body regions and among the size classes of Rhamdia quelen was compared. The host size classes were defined by cluster analysis, using weight and total length as attributes. These values were related with environmental characteristics and host biological data. In all the host size classes H and PeF, CF and PF, and P and AF regions pairs, showed similar density (tested by X² test). The hosts with 30-36 cm showed largest parasite density in all the regions, except in CAV. Fishes smaller than 11 cm did not show significant differences of parasite density in all body regions. In the other classes, H was the most infected region, followed by PeF. The results suggested that the environmental conditions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinostomum complanatum; Rhamdia quelen; Metacercariae density; Jundiá; South Brazil; Fish disease; Aquaculture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500016

Comparative study of a new alkaline L-methioninase production by Aspergillus ustus AUMC 10151 in submerged and solid-state fermentation
Provedor de dados: 52
Autores: Abu-Tahon,Medhat Ahmed; Isaac,George Saad.
Twenty four fungal species were screened for their ability to produce alkaline L-methioninase on methionine-glucose liquid medium. Aspergillus ustus AUMC 10151 displayed the highest yield of enzyme (10.8 U/mg protein), followed by A. ochraceus and Fusarium proliferatum. Upon optimization of the submerged fermentation (SmF)conditions, the maximum enzyme yield (18.23 U/mg protein) was obtained on a medium containing L-methionine (0.5%), sucrose (0.95%), KH2PO4 (0.1%) and 175 rpm. Seven agro-industrial by-products were screened as substrates for L-methioninase production under solid-state fermentation (SSF). Wheat bran resulted 38.1 U/mg protein, followed by rice bran (27.6 U/mg protein) and soya bean meal (26.6 U/mg protein). Maximum alkaline L-methioninase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-methioninase; Aspergillus ustus; Submerged fermentation; Solid state fermentation; Wheat bran.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100502

Elaboration of sausage using minced fish of Nile tilapia filleting waste
Provedor de dados: 52
Autores: Oliveira Filho,Paulo Roberto Campagnoli de; Maria Netto,Flavia; Ramos,Kazumi Kawazaki; Trindade,Marco Antônio; Viegas,Elisabete Maria Macedo.
The objective of this study was to evaluate the inclusion of minced fish (MF) (0, 20, 40, 60, 80 and 100%), obtained from Nile tilapia filleting waste, in sausage and determine their physicochemical, nutritional and sensory properties. The sausages showed a decrease in protein and increase in fat content with increasing inclusion of MF. The nutritional quality of the products was high, with digestibility over 85%. The parameters of texture instrumental and yellow color (b*) decreased with the increasing inclusion of MF. The sensory evaluation of the color showed that the maximum level of inclusion of MF was not well accepted by the panelists. The sausage with the best acceptance for the flavor attribute was those with 60% of MF. The results showed good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oreochromis niloticus; Sausage; Nutritional quality; Minced fish; Sensory acceptance; Texture.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600015

Tucum-do-Pantanal (Bactris setosa Mart.): Physicochemical Characterization of Almonds, Press Cake and Crude Oil
Provedor de dados: 52
Autores: Aydos,Leonardo Recena; Amaral,Luane Aparecida do; Souza,Gabriel Henrique Oliveira de; Cavalheiro,Leandro Fontoura; Vargas,Márcio Olívio Figueiredo; Rafacho,Bruna Paola Murino; Nazário,Carlos Eduardo Domingues; Oliveira,Rodrigo Juliano; Macedo,Maria Ligia Rodrigues; Santos,Elisvânia Freitas dos.
Abstract Brazil has high diversity of native fruits with high nutritional and biochemical value. Bactris setosa Mart. (tucum-do-Pantanal) stands out by its oil-rich almond. This study aimed to determine the physicochemical characteristics of tucum-do-Pantanal almond and its by-products: press cake and crude oil. The almond of tucum-do-Pantanal had total weight of 0.81g, lager diameter 10.87mm, small diameter 8.21mm, height 12.50, weight of almond 0.38g, weight of endocarp 0.25g. In relation to the chemical analysis, the cake had higher ash, protein and carbohydrate contents than the almond. On the other hand, the content of moisture, lipids and calories were higher in the almond. The press cake showed 636.80 g kg-1 of total fiber. The fatty acids that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food analysis; Fatty acids; Tucum-do-Pantanal; Pantanal.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100511

Anti-CD3 Antibody Ameliorates Transfusion-Associated Graft-Versus-Host Disease in a Chemotherapy-Based Mouse Model With Busulfan and Fludarabine
Provedor de dados: 52
Autores: Li,Xiaofan; Song,Qingxiao; Hu1,Wanyu; Wan,Bo; Huang,Qinghua; Li,Qing.
ABSTRACT To establish a transfusion-associated graft-versus-host disease (TA-GVHD) mouse model with busulfan and fludarabine for effective treatment evaluation. BALB/c (H-2d) mice were injected with busulfan (15 mg/kg) and fludarabine (30 mg/kg) twice a day for 4 days. The mice were transfused with 106 T cell-depleted bone marrow (TCD-BM )and cells in different groups 3 days after chemotherapy: syngeneic BALB/c, MHC minor mismatch DBA/2 (H-2d), or MHC major mismatch C57BL/6(H2-b). Recipient BALB/c mice were injected with either blood only or blood+splenocyte. TA-GVHD was monitored in terms of body weight loss, clinical scores, and survival. Dexamethasone (50 mg/kg), cyclophosphamide (50 mg/kg), cyclosporine A (30 mg/kg), and anti-CD3 (1 mg/kg) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transfusion-associated graft-versus-host disease; Fludarabine; Busulfan; Animal model; Major histocompatibility complex.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100305

Proline content and protein patterns in Eucalyptus grandis shoots submitted to high and low temperature shocks
Provedor de dados: 52
Autores: Souza,Gustavo M.; Cardoso,Victor J. M.; Gonçalves,Antonio N.
Proline content and protein patterns changes in response to temperature shocks of both acclimated and non acclimated E. grandis shoots cultivated in vitro were investigated. Analysis of soluble proteins through SDS-PAGE and proline were carried out after 12h at 12ºC (cold acclimation) or 33ºC (heat acclimation), and immediately after temperature shocks at 41ºC and 0ºC. Analyses were also performed 24h after the temperature shocks (recovery period). Temperature treatment at 0ºC did not change soluble protein patterns both in acclimatized and non acclimatized plants, whereas cold temperature induced high proline levels, which kept relatively high after recovery period. Three novel, probably HSPs, proteins (90.5, 75 and 39 kDa) were observed in both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat shock protein; Proline; Recovery; Stress; Temperature shock.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300004

Morphology of olfactory sensilla and its role in host plant recognition by Neomegalotomus parvus (Westwood) (Heteroptera: Alydidae)
Provedor de dados: 52
Autores: Ventura,Maurício Ursi; Panizzi,Antônio Ricardo.
The external morphology of the sensilla, including pores and permeable points on the cuticle of the antennae of adults of Neomegalotomus parvus (West.) (Heteroptera: Alydidae) was investigated with regard to their olfactory function. Behavioural evidence of olfaction, the importance of the different antennal segments in locating the host under still-air, and the responses of the insects to plants' scents and to pheromones in wind assays were also studied. With the scanning electronic microscope mechanoreceptor sensilla were found on the four segments of the antennae of N. parvus. The density of the sensilla was greater on the 4th segment. On the apical segment, sensilla with olfactory morphological traits were observed. Bugs with intact antennae located...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sensilla; Host plant; Behaviour; Antenna.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500012

Recolonization of experimental gaps by the mussels Brachidontes darwinianus and B. solisianus in a subtropical rocky shore
Provedor de dados: 52
Autores: Tanaka,Marcel Okamoto.
In this study, the recolonization speed of the mussels Brachidontes darwinianus and B. solisianus was compared to evaluate their responses to gaps opened by disturbances in the rocky shore. B. darwinianus was much faster than B. solisianus, rapidly closing gaps in the mussel bed. When they co-occurred, distribution patterns of these species were related not only with distinct physiological tolerances but also with responses to disturbance. B. darwinianus was more tolerant to low salinities, dominated estuarine zones generally occuring lower in the midlittoral, and presented a faster response to opened gaps.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bivalve; Intertidal; Rocky shore; Zonation; Mytilidae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100015

Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties
Provedor de dados: 52
Autores: Huang,Shengwei; Deng,Guanjun; Yang,Ying; Wu,Zhengyan; Wu,Lifang.
ABSTRACTIn this study, a potential novel cellulolytic bacteriumArthrobacter sp. HPG166 was isolated from the hindgut of root-feeding larvaeHolotrichia parallela. Optimization of fermentation factors for endoglucanase production byArthrobacter sp. HPG166 was carried out via response surface methodology. Sodium carboxymethylcellulose 1.19% (w/v) and beef extract 0.35% (w/v) were the ideal combination of carbon and nitrogen sources for enzyme production; the optimum temperature and pH for cellulase production were 34°C and pH 8.0 respectively. Under the optimized fermentation conditions, the maximum endoglucanase activity of 1.411 U mL-1 was obtained. The crude endoglucanase was thermotolerant as it retained 50.31% of its activity after incubation at 70°C for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scarab larvae; Cellulolytic bacteria; Response surface methodology; Thermotolerant cellulases; Effects of Metal ions.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500692

Characterization of the penicillin G acylase from Bacillus megaterium ATCC 14945
Provedor de dados: 52
Autores: Souza,Vanessa Ribeiro de; Silva,Ana C. G.; Pinotti,Laura Marina; Araújo,Heloísa Sobreiro Selistre; Giordano,Raquel de Lima Camargo.
The purpose of this work was to characterize the enzyme penicillin G acylase (PGA) produced by Bacillus megaterium. Purification of the enzyme by ultra/diafiltration did not allow the detection of the PGA band by SDS-PAGE electrophoresis due to the high content of remaining proteins. However, using the DNA of the microorganism, it was possible to replicate the genes of the two B. megaterium PGA reported in literature, showing that the enzyme consisted of two sub-units, having 245 and 537 amino acids each and an average molecular mass of 26950 and 59070 Da, respectively. The parameters studied were: 1) the influence of temperature in the 25-60(0)C range, 2) pH in the 5-10 range and 3) substrate concentration, this was tested to obtain results on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillin G acylase; Bacillus megaterium; Characterization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400013

Lead Nitrate Induced Testicular Toxicity in Diabetic and Non-Diabetic Rats: Protective Role of Sodium Selenite
Provedor de dados: 52
Autores: Apaydin,Fatma Gokce; Kalender,Suna; Bas,Hatice; Demir,Filiz; Kalender,Yusuf.
Among heavy met als, lead is one of the common pollutants found in the environment and biological system. In the present study, streptozotocin-induced diabetic and normal non-diabetic male Wistar rats were given sodium selenite (1.0 mg/kg bw), lead nitrate (22.5 mg/kg bw) and sodium selenite plus lead nitrate (1.0 mg/kg+22.5 mg/kg bw, respectively) through gavage. At the end of 4th week, malondialdehyde (MDA) levels, antioxidant enzyme activities [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST)], and histopathological changes of testes were investigated compared to the control group. No significant differences were observed between the control and sodium selenite treated groups. However, lead...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Testicular toxicity; Lead nitrate; Sodium selenite; Streptozotocin; Oxidative stress; Histopathology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100068

Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells
Provedor de dados: 52
Autores: Mousinho,Kristiana Cerqueira; Correia,Marília Bezerra Libório; Silva,Jailson Oliveira da; Magnata,Simey de Souza Leão Pereira; Souza,Ivone Antônia de; Catanho,Maria Teresa Jansem de Almeida.
The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of Tc-99m and TCA at 5%, determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ricinus communis L.; Labeling of red blood cells; Osmotic fragility; Technetium-99m; Morphology of cells.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600008

Effect of buctril super (Bromoxynil) herbicide on soil microbial biomass and bacterial population
Provedor de dados: 52
Autores: Abbas,Zafar; Akmal,Muhammad; Khan,Khalid Saifullah; Fayyaz-ul-Hassan,.
The present study aimed to evaluate the effect of bromoxynil herbicide on soil microorganisms, with the hypothesis that this herbicide caused suppression in microbial activity and biomass by exerting toxic effect on them. Nine sites of Punjab province (Pakistan) those had been exposed to bromoxynil herbicide for about last ten years designated as soil 'A' were surveyed in 2011 and samples were collected and analyzed for Microbial Biomass Carbon (MBC), Biomass Nitrogen (MBN), Biomass Phosphorus (MBP) and bacterial population. Simultaneously, soil samples from the same areas those were not exposed to herbicide designated as soil 'B' were taken. At all the sites MBC, MBN and MBP ranged from 131 to 457, 1.22 to 13.1 and 0.59 to 3.70 µg g-1 in the contaminated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass; Buctril super; Bacteria; Effect; Population.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100002

Thermoregulation in colonies of africanized and hybrids with Caucasian, Italian and Carniolan Apis mellifera honey bees
Provedor de dados: 52
Autores: Toledo,Vagner de Alencar Arnaut de; Nogueira-Couto,Regina Helena.
This experiment was carried out to study the internal temperature regulation of a colony of Africanized honey bees (AFR), compared with hybrid Caucasian (CAU), Italian (ITA), and Carniolan (CAR) bees, during the period of one year and different size hives located in a sub-tropical region. The instant internal temperature, 33.7 ± 1.5° C for the AFR, 33.5 ± 1.4° C for the CAU, 33.7 ± 1.5° C for the ITA and 33.8 ± 1.4° C for the CAR, did not show any significant difference (P&amp;gt;0.05). The maximum temperature (36.1 ± 2.3° C) was statistically different (P&lt;0.05) from the minimum (27.6 ± 5.3° C). There was no difference (P&amp;gt;0.05) in the mean internal temperature, between the nucleus (31.7 ± 6.3° C) and the brood nest (32.1 ± 5.3° C) measured...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temperature; Honey bee; Apis; Thermoregulation; Hive; Hybrids.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400007

Selenium protective activity against aflatoxin B1 adverse affects on Drosophila melanogaster
Provedor de dados: 52
Autores: Uysal,Handan; Agar,Güleray.
The aim of this work was to investigate the effects of AFB1 and AFB1+Se4+ on various developmental stages of Drosophila melanogaster were investigated. Both different concentrations of AFB1 and Se4+ applied with AFB1 were fed during the flies developmental period (egg, larva and pupae). When F1 progeny of control and application groups were compared with each other, AFB1 was found to have extending the process of metamorphosis and decreasing the total number of offsprings. But, these negative effects were inhibited with selenium treatment at different concentrations (4.0 and 8.0 ppm). These results suggested that selenium could effectively inhibit AFB1- induced abnormalities of the developmental stages of D.melanogaster.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Selenium; Aflatoxin B1; Developmental stages; Protective effect; D. melanogaster.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200009

Development of an algorithm for tip-related artifacts identification in AFM biological film imaging
Provedor de dados: 52
Autores: Bernardes-Filho,Rubens; Assis,Odilio Benedito Garrido de.
One major drawback identified in atomic force microscopy imaging is the dependence of the image's precision on the shape of the probe tip. In this paper a simple algorithm is proposed to provide artifact identification signaling in-situ tip features in atomic force microscopy images. The base of the identifications lied when the angle formed between two scanned points was kept the same as the tip sweeps a certain length of the sample. The potential of the described method was illustrated on a chitosan polysaccharide film. The images produced were compared to evaluate tip-artifact regions. This algorithm showed promise as a tool in the measurement and characterization fields to separate true images from artificial images in probe microscopy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probe microscopy; AFM; Images tip artifacts; Biological film.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500020

Differential pathogenicity of Metarhizium anisopliae and the control of the sugarcane root spittlebug Mahanarva fimbriolata
Provedor de dados: 52
Autores: Tiago,Patricia Vieira; Souza,Hilton Marcelo de Lima; Moysés,Juliano Borsato; Oliveira,Neiva Tinti de; Lima,Elza Áurea de Luna Alves.
In order to assess the effectiveness of Metarhizium anisopliae var. anisopliae (Metschnikoff) Sorokin isolates in controlling the sugarcane root spittlebug Mahanarva fimbriolata (Stal) (Hemiptera: Cercopidae), nine isolates obtained from a single geographical region were studied. 'Confirmed cumulative' and 'corrected cumulative' spittlebug mortality rates were measured for each of the isolates. Based on the confirmed mortality curve, the isolates URM5946, URM5951 and URM6033 were considered to be potentially the most effective in a biological control program for M. fimbriolata.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Entomopathogenic fungus; Metarhizium anisopliae; Mahanarva fimbriolata.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300002

Adsorption, immobilization and activity of cellulase in soil: the impacts of maize straw and its humification
Provedor de dados: 52
Autores: Sinegani,Ali Akbar Safari; Sinegani,Mahboobe Safari.
The present work aimed to study some aspects of sorption and immobilization of cellulase molecules on soil components by the analysis of the reactions of cellulase in a soil treated with different levels of maize residue and incubated for 90 days. The analysis of variance showed that the effects of the treatments of maize straw, incubation time and their interaction on cellulase adsorption, desorption and immobilization were statistically significant. The adsorption and immobilization capacities of soil by application of maize straw increased significantly. However they decreased with decreasing the soil organic matter (SOM) after 45 days of incubation. The desorption of adsorbed cellulase molecules from the soil by washing with distilled water depended on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize straw; Humification; Cellulase; Sorption; Immobilization; Activity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600001

Broiler chicken PSE (Pale, Soft, Exudative) meat and water release during chicken carcass thawing and brazilian legislation
Provedor de dados: 52
Autores: Kato,Talita; Barbosa,Claudia Freitas; Ida,Elza Iouko; Soares,Adriana Lourenço; Shimokomaki,Massami; Pedrao,Mayka Reghiany.
The objective of this work was to investigate the relationship between poultry PSE meat and water loss of frozen chicken carcass in two experiments. The first experiment was carried out in commercial abattoir. Poultry carcass were classified as PSE meat (n=59) (pH &lt;5.8) and control samples (n=55) (pH &amp;gt;5.8). Water absorption and drip test were performed according to the Brazilian legislation methodologies. The second experiment was carried out with commercial whole five brands frozen carcasses purchased from the local supermarkets (n=30) and analyzed for pH, water holding capacity (WHC) and drip test. PSE poultry meats absorbed 3.59% of water during the processing similar to the control samples; however, released 0.38% more water during thawing of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drip loss; Water absorption; Water holding capacity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600015

Development of a Prototype for Measurement of Electrical Energy
Provedor de dados: 52
Autores: Costa,Alysson; Ciniello,Fernanda; Grzybowsk,Gustavo; Betini,Roberto C.
ABSTRACT The present work presents the development of a system of measurement of electric energy consumption. This system shows the consumer the amount of energy consumed and its respective monetary value in real time. The prototype was used in a case study to validate its operation. Data were collected to discuss the results obtained. In order to verify if the results obtained in the prototype were correct, several measurements of energy consumption were made in different residential equipment. The consumption values measured by the prototype were compared with the values obtained by the certified and calibrated energy meter provided by COPEL and were satisfactory.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meter of energy; Consumption; Electric energy; Energy efficiency.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200216

Assessment of the Potential Role of Silymarin Alone or in Combination with Vitamin E and/ or Curcumin on the Carbon Tetrachloride Induced Liver Injury in Rat
Provedor de dados: 52
Autores: Al-Rasheed,Nouf; Faddah,Laila; Sharaf,Iman A; Mohamed,Azza M; Al-Rasheed,Nawal; Abdelbaky,Nayira.
ABSTRACT The aim of this study was to investigate the effective role of silymarin either alone or in a combination with vitamin E and/or curcumin against the toxic impact of carbon tetrachloride (CCl4) induced liver injury The results revealed that administration of silymarin alone or in a combination with vitamin E and/or curcumin for 21 consecutive days, 24 h after CCl4 injection to rats, markedly ameliorated DNA damaged and apoptosis markers in rat livers, proinflammatory markers including tumor necrosis factor- α (TNF- α) and C-reactive protein (CRP ) in rat livers as well as interleukin 6 (IL-6), interferon gamma (IFN-γ) and vascular endothelial growth factor (VEGF) in rat sera. These treatments also could ameliorated the alteration in cytochrome P450...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silymarin; Vitamin E; Curcumin; Carbon Tetrachloride.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600833

Crude glycerol from biodiesel industry as substrate for biosurfactant production by Bacillus subtilis ATCC 6633
Provedor de dados: 52
Autores: Sousa,Marylane de; Dantas,Iuri Torquato; Felix,Anne Kamilly Nogueira; Sant'Ana,Hosiberto Batista de; Melo,Vânia Maria Maciel; Gonçalves,Luciana Rocha Barros.
Glycerol, a co-product of the biodiesel industry, may be a suitable raw material for the production of high added-value compounds by the microorganisms. This study aimed to use the glycerol obtained from the biodiesel production process as the main carbon source for biosurfactant production by Bacillus subtilis ATCC 6633. Results indicated that the strain lowered the surface tension of the cell-free fermented broth to 31.5 ± 1.6 mN/m, indicating the production of biosurfactant. The critical micelle concentration (CMC = 33.6 mN/m) obtained was similar to the previously reported for biossurfactants isolated from other Bacillus. The produced biosurfactant was able to emulsify n-hexadecane and soybean oil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactant; Biodiesel; Glycerol and Surfactin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200019

Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431
Provedor de dados: 52
Autores: Mohansrinivasan,V.; Devi C.,Subathra; Deori,Meenakshi; Biswas,Ananya; Naine S,Jemimah.
In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE). Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria. Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells, viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GSE (Grape seed extract); Phytochemical; FTIR; GCMS; Skin cancer cell line; Anticancer agents.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400540

Optimization of thermostable α- amylase production by Streptomyces erumpens MTCC 7317 in solid-state fermentation using cassava fibrous residue
Provedor de dados: 52
Autores: Shaktimay,Kar; Datta,Tapan Kumar; Ray,Ramesh Chandra.
Production of α- amylase under solid state fermentation by Streptomyces erumpens MTCC 7317 was investigated using cassava fibrous residue, one of the major solid waste released during extraction of starch from cassava (Manihot esculenta Crantz). Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e., incubation period (60 h), moisture holding capacity (60%) and temperature (50(0)C) on enzyme production by applying a full factorial Central Composite Design. Varying the inoculum concentration (5-25%) of S. erumpens showed that 15% inoculum (v/w, 2.5 x 10(6) CFU/ml) was the optimum for α- amylase production. Among the different nitrogen sources supplemented, beef extract was most suitable for enzyme production. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α- amylase; Cassava fibrous residue; Optimization; Response surface methodology; Solid state fermentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200008

Loss of macronutrients (N, P, K) in the hydrographic basin of the River Ivaí, an affluent of the River Paraná
Provedor de dados: 52
Autores: Guiraud,Deborah Maria Corrêa; Lenzi,Ervim; Luchese,Eduardo Bernardi; Fávero,Luzia Otília Bortotti.
Influence of some environmental variables in the loss of macronutrients N, P, K, from the hydrographic basin of the River Ivaí were analysed. Water samples of the river were monthly collected for five consecutive days during a year. In terms of total intervals of average monthly values and total average values experiment results, in mg L-1, were: N (0.32-3.22 and 1.65); K (0.73-2.69 and 1.38); P (not detected-0.39 and 0.076); COD (0.21-36.0 and 12.8); O2(dis) (1.89-8.40 and 5.43); and temperature (&amp;#186C) (16.0-30.8 and 24.6); pH (5.18-8.50 and 7.15). Statistical analysis of data showed that quantity of macronutrient (N, K, P) carried off from the hydrographic basin of the River Ivaí were directly correlated to fluviometric levels (caused by rainfall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erosion; Macronutrients; Hydrographic basin; Environment.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400019

Bark Extract of Bathysa cuspidata in the Treatment of Liver Injury Induced by Carbon Tetrachloride in Rats
Provedor de dados: 52
Autores: Gonçalves,Reggiani Vilela; Matta,Sérgio Luis Pinto da; Novaes,Rômulo Dias; Leite,João Paulo Viana; Peluzio,Maria do Carmo Gouveia; Vilela,Emerson Ferreira.
The aim of this study was to investigate the effect of bark extract Bathysa cuspidata (BCE) in the reconstitution of hepatic parenchyma and stroma after dysfunction induced by the CCl4. Liver lesions were induced by intraperitoneal administration of CCl4 (1 mL/kg) every 48 h for 12 days. The animals were treated with B. cuspidata extract (BCE) administered by gavage for another 12 days. Forty-nine rats were randomized into seven treatment groups with seven animals receiving CCl4, BCE (200, or 400 mg/kg) and the vehicle DMSO alone, or in different combinations. The extract alone showed no evidence of hepatic toxicity. In general, rats acutely exposed to CCl4 without the treatment with BCE presented high ALT and AST serum levels and high tissue content of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant defense; Biotechnology; Hepatotoxicity; Phytotherapy; Pathology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400504

Effects of Sodium Nitrate and Mixotrophic Culture on Biomass and Lipid Production in Hypersaline Microalgae Dunaliella Viridis Teod
Provedor de dados: 52
Autores: Kharati-Koupaei,Mansour; Moradshahi,Ali.
To access the potential application of Dunaliella viridis Teod. for biofuel production, the effects of culture media composition on biomass and lipid content of this microalgae were investigated. Measured at the 20 th day, sodium nitrate at 5.0 mM augmented biomass production by 26.5 percent compared to control (1 mM sodium nitrate). Total lipids expressed as µg mL-1 of culture also increased with increase in nitrate concentration up to 5.0 mM sodium nitrate, whereas when expressed on the per cell basis, total lipids stayed relatively constant at most of the tested nitrate concentrations except at 0.5 mM which was 31.4 percent higher compared to 1.0 mM nitrate. At 5.0 mM sodium nitrate, by using 20 g L-1 of glucose in mixotrophic culture of D. viridis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dunaliella viridis; Mixotrophic culture; Biofuel; Biomass; Lipid content; Hormones.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100201

Anti-Helicobacter pylori and Anti-inflammatory Properties of Eugenia uniflora L.
Provedor de dados: 52
Autores: Monteiro,Jessica Raquel Borges; Ardisson,Juliana Santa; Athaydes,Brena Ramos; Gonçalves,Rita de Cássia Ribeiro; Rodrigues,Ricardo Pereira; Kuster,Ricardo Machado; Kitagawa,Rodrigo Rezende.
Abstract Helicobacter pylori is a bacterium that reaches half of the world population and it’s recognized as the main cause of chronic gastritis and peptic ulcer. In this study, we evaluated the anti-H. pylori, antioxidant and immunomodulatory activities of the methanolic (MeOH) extract of Eugenia uniflora leaves and chemical profile. Anti-H. pylori activity was evaluated by spectrophotometric broth microdilution technique by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), in addition to the evaluation of the effect on the urease enzyme. The antioxidant activity was evaluated by capturing O2 •-, HOCl e NO• radicals. The immunomodulatory effect was evaluated on the cytokines TNF-α, IL-6 and on nitric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenia uniflora; Helicobacter pylori; Antioxidant; Immunomodulation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100424

Population structure of Phalloceros caudimaculatus (Hensel, 1868) (Cyprinodontiformes, Poeciliidae) collected in a brook in Guarapuava, PR
Provedor de dados: 52
Autores: Wolff,Luciano Lazzarini; Hreciuk,Ericsson Ricardo; Viana,Douglas; Zaleski,Tânia; Donatti,Lucélia.
The population structure of Phalloceros caudimaculatus was studied in a brook (sites A and B) existing in the CEDETEG - Guarapuava/PR. Fourteen length classes of 3 mm amplitude were defined for P. caudimaculatus. The widest length amplitude and the average and maximum sizes were observed in females. Females were significantly more predominant in the whole sample and more frequent in all length classes. The sexual proportion data, showed that the number of females was superior to that of males throughout most of the year. Young occurrence peaks were registered in November and March. Pregnant females were present from March to April, from July to August and in January, characterizing the reproductive season. At sampling site A, a higher occurrence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Ecology; Fresh-water.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300008

Mentha piperita effects on wistar rats plasma lipids
Provedor de dados: 52
Autores: Barbalho,Sandra Maria; Spada,Ana Paula Machado; Oliveira,Erick Prado de; Paiva-Filho,Márcio Emilio; Martuchi,Karla Aparecida; Leite,Náyra Coelho; Deus,Renata Maeda; Sasaki,Vivianne; Braganti,Lucas Silva; Oshiiwa,Marie.
The aim of this study was to demonstrate the effects of Mentha piperita juice and tea on plasma lipids of Wistar rats. The animals were divided in control group (CG) and four treated groups: TG1 treated with tea and TG2 treated with juice (both receiving commercial rat chow); TG3 and TG4 were fed with supplemented fat chow (with hydrogenated fat and soybean oil) and treated respectively with tea and juice. Both tea and juice were administered by intra-gastric route (gavage) two times a day for 30 days. TG2 and TG4 animals showed significant decrease in triacylglycerols and increase in HDL-c levels. TG1 and TG2 also showed lower cholesterol values. The levels of LDL-c increased in CG but decreased in the treated groups. Animals of TG1, TG2 and TG4 showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mentha piperita; Cholesterol; HDL-c; LDL-c; Triacylglycerol.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500011

Effects of the application of sewage sludge and fowl manure on soils of Paraná state in maize plants (Zea mays L.) as a macro-nutrients source
Provedor de dados: 52
Autores: Gobbi,Marlene A.; Pigozzo,Ana T. Jordão; Lenzi,Ervim; Luchese,Eduardo B.
The potential of two organic residues as sources of macro-nutrients in three types of soils of the third plateau of Paraná state was studied. Treatment consisted of a dose (38 t.ha-1) of fowl manure and sewage sludge neutralized by a 3:1 (CaO+MgO) mixture. Fowl manure was kept in natura. Maize (Zea mays L.)was reference plant. After 30 days of seeding, the aerial parts of the plants were cut. They were dried at 70 0C till constant weight was obtained, then weighted and ground. Analyses were undertaken after nitric-perchloric digestion of the samples. Macro-nutrient levels in soils and in plants were determined by atomic absorption spectrometry, flame technique. Results showed that production of dry matter was higher when fowl manure was used. Levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sewage sludge; Macro-nutrients; Maize culture; Soil fertility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200003

Morphology of the male gonads of the spiny lobster Panulirus laevicauda (Latreille, 1817)
Provedor de dados: 52
Autores: Lima,Ana Valêsca Pinto de; Gesteira,Tereza Cristina Vasconcelos.
The present study represented a contribution to the knowledge of the cytological and histological aspects of decapods' reproductive system, describing male germ cells of the spiny lobster Panulirus laevicauda. Seventy-one specimens of different sizes were caught off Fortaleza (Ceará, Brazil). Their testes were removed and fixed in Bouin solution, then, after 24 hours, dehydrated, cleared and embedded in the paraffin. Sections (4 µm thick) were stained with hematoxylin-eosin. The testes appeared macroscopically as a pair of long and highly convoluted tubes joined by a transversal commissure giving the organ an H-like shape. Microscopically, supporting cells and germ cells (spermatogonia I and II, spermatocytes, spermatids and spermatozoa) were seen in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histology; Male reproductive system; Spiny lobster; Panulirus laevicauda.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400008

Reproductive biology of Oligosarcus argenteus (Gunther, 1864) adult males and description of the gonadal maturation stages
Provedor de dados: 52
Autores: Matt,Sérgio Luis Pinto da; Gomes,Marcos de Lucca Moreira; Andrade,Dálcio Ricardo de.
Oligosarcus argenteus belongs to the Acestrorhynchinae subfamily, being restricted to South America, and found in several Brazilian hydrographic basins, in lotic and lentic environments, where they are able to reproduce. With the purpose of studying the reproductive biology of the males from this species, many morphological parameters were analyzed during a 24 month period, as well as characterizing the different testicular maturation stages. A maturity scale, with three stages (I - Initial Maturing, II - Intermediate Maturing, III - Final Maturing) was proposed for the adult males of Oligosarcus argenteus. The reproductive period was established by the bimonthly frequency of spermatogenesis and by the gonadal maturation stages.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproductive cycle; Histology; Testis; Oligosarcus argenteus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100016

Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress
Provedor de dados: 52
Autores: Shao,Huanhuan; Yong,Bin; Xu,Pan; Zheng,Haiyan; Liao,Ruoxing; Wang,Xiaoyan; Li,Xinyu; Zhang,Li; Shen,Jiabin.
ABSTRACT Phytoene synthase (PSY) is the rate-limiting enzyme for carotenoid biosynthesis. To date, several studies focused on PSY genes in the context of abiotic stress responses. In this study, two phytoene synthase encoding genes, IbPSY1 and IbPSY2, were identified from a published transcriptome and bioinformatic analysis showed that they shared conserved domains with phytoene synthases from other plants. The IbPSY1 gene was cloned and carefully characterized. Digital gene expression profiling (DGE) showed that the highest transcription level of IbPSY1 was in young leaves, and the lowest level was in stems. In vivo expression levels of IbPSY1 under abiotic stress were observed to be highest in stems at day 11. Over-expression of IbPSY1 in Escherichia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipomoea batatas; Phytoene synthase; Abiotic stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100422

Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
Provedor de dados: 52
Autores: Simões,Claudia; Albarello,Norma; Callado,Cátia Henriques; Castro,Tatiana Carvalho de; Mansur,Elisabeth.
This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), a -naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D). Nodular calli were produced from both explant types in the presence of 4.5 and 9.0 µM 2,4-D. Embryo development and maturation were achieved when calli from stem explants were transferred to media containing a ten-fold reduction of 2,4-D concentration initially used (0.45 and 0.90 µM). Leaf-derived calli did not form embryos with the same treatments. The highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auxin; Embriogenic callus; Histology; Phytagel; Somatic embryos.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300024

Novel Microsatellite Markers for Brazilian Mangrove Oysters ( Crassostrea gasar ) and their Cross-Amplification in Crassostrea rhizophorae
Provedor de dados: 52
Autores: Baldez,Renata do Socorro Corrêa; Melo,Mauro André Damasceno; Sampaio,Iracilda; Tagliaro,Claudia Helena.
ABSTRACT A microsatellite CT/GT enriched genomic library was developed for Crassostrea gasar and twelve new polymorphic loci were isolated and characterized. The markers were successfully amplified from 25 individuals of Crassostrea gasar and 11 cross-amplified individuals of Crassostrea rhizophorae. There was no evidence of linkage between loci in either species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Genetic markers; Cupped oyster; Population structure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100426

Detection of Clostridium sp. and its Relation to Different Ages and Gastrointestinal Segments as Measured by Molecular Analysis of 16S rRNA Genes
Provedor de dados: 52
Autores: Mirhosseini,Seyed Ziaeddin; Seidavi,Alireza; Shivazad,Mahmoud; Chamani,Mohammad; Sadeghi,Ali Asghar; Pourseify,Reza.
The objective of this study was to establish a specific, sensitive and rapid PCR approach for the detection of Clostridium sp. at the genus level. Clostridium sp. in the duodenum, jejunum, ileum and cecum of broiler chickens were analyzed by 16S rRNA genes. The PCR detected the presence of Clostridium spp. in naturally contaminated intestinal samples. For the total gastrointestinal segments, 53.125, 65.625 and 59.375% samples were positive for naturally occurring Clostridium spp. at the ages 4, 14 and 30d, respectively. Analysis of the microbial contents indicated that Clostridium sp. was not consistently detected in all intestinal segments. These results can put in evidence the hypothesis that Clostridium spp. may be interfering in health and performance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium spp.; Broiler; Gastrointestinal; Age; 16S rRNA genes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100009

Purification and characterization of an anti-cancer enzyme produced by marine Vibrio Costicola under a novel solid state fermentation process
Provedor de dados: 52
Autores: Prabhu,G. Nagendra; Chandrasekaran,M.
L - Glutaminase, a therapeutically and industrially important enzyme, was produced from marine Vibrio costicola by a novel solid state fermentation process using polystyrene beads as inert support. The new fermentation system offered several advantages over the conventional systems, such as the yield of leachate with minimum viscosity and high specific activity for the target product besides facilitating the easy estimation of biomass. The enzyme thus produced was purified and characterised. It was active at physiological pH, showed high substrate specificity towards L - glutamine and had a Km value of 7.4 x 10-2 M. It also exhibited high salt and temperature tolerance indicating good scope for its industrial and therapeutic applications.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L- Glutaminase; Vibrio costicola; Solid state fermentation; Inert support; Purification and characterisation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300015

Profile of the alcohols produced in fermentations with malt contaminated with trichothecenes
Provedor de dados: 52
Autores: Reinehr,Christian Oliveira; Furlong,Eliana Badiale.
In order to study the influence of mycotoxins on the production of alcohols, a fermentative process on a laboratorial scale was simulated. Malt was contaminated with deoxynivalenol and T-2 in different ratios (up to 500 ppb), according to a 3² factorial design, and the alcohols obtained after the fermentation were determined through gas chromatography. The results showed that trichothecenes influenced the profile of the alcohols produced by Saccharomyces cerevisiae during the fermentative process of malt, especially the profile of methyl and isoamyl alcohols.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deoxynivalenol; Fermentation; Malt; T-2 toxin; Trichothecenes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400013

Comparison of two lipid extraction methods produced by yeast in cheese whey
Provedor de dados: 52
Autores: Castanha,Rodrigo Fernandes; Morais,Lilia Aparecida Salgado de; Mariano,Adriano Pinto; Monteiro,Regina Teresa Rosim.
This work aimed to evaluate nine strains of yeast, previously identified as good producers of lipids in honey medium, for selecting the most suitable strain for the production of lipids in cheese whey medium and compared two well known extraction methods of lipids from the culture medium. The highest yield of total lipids was 1.27 g.L-1 produced by Cryptococcus laurentii 11. A comparison was made between the two culture media: cheese whey and liquid YEPG, and two lipid extraction methods: Bligh and Dyer and Folch et al. for C. laurentii. The experiments were performed with 2² full factorial design using two factors and two levels. Lipid content was higher in cheese whey and there was no difference in the extraction methods statistically. The method of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cheese whey; Yeast; Lipid extraction; Microbial oil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400013

Mortality of Bemisia tabaci biotype B (sternorrhyncha: aleyrodidae) adults by aliphatic and aromatic synthetic sucrose esters
Provedor de dados: 52
Autores: Alves,Mariangela; Boscolo,Maurício; Fernandes,Odair Aparecido; Nunes,Maria Andréia.
The B-strain of Bemisia tabaci Gennadius is a key pest of several crops and chemical control is the main control method used by growers, although reduction in efficacy due to insecticide resistance has already been reported. The aim of this work was to investigate the insecticidal effect of an array of synthetic sucrose esters with the aliphatic and aromatic groups on whitefly adults. Sucrose butyrate, caprate, octanoate, palmitate, oleate, octaacetate, phthalate, benzoate, and sucrose diacetate hexaisobutyrate were tested. The solutions were prepared and applied on the adults caught on yellow sticky traps using the Potter spray tower. Long-chains sucrose aliphatic esters were more effective against the silverleaf whiteflies and the highest mortality was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sucroesters; Silverleaf whitefly; Pest control; Integrated pest management.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600005

Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale
Provedor de dados: 52
Autores: Elsayed,Elsayed Ahmed; Omar,Hoda Gamal; El-Enshasy,Hesham Ali.
ABSTRACTOxytetracycline (OTC) production byStreptomyces rimosus was studied in batch and fed-batch cultures in shake flask and bioreactor levels using semi-defined medium. First, the effect of glucose concentration on OTC production and growth kinetics was studied intensively. The optimal glucose concentration in the medium was 15 g/L. Higher glucose concentrations supported higher biomass production by less volumetric and specific antibiotic production. Based on these data, cultivations were carried out at semi-industrial scale 15 L bioreactor in batch culture. At bioreactor level, cell growth and OTC production were higher compared to the shake flask culture by about 18 and 38%, respectively. During the bioreactor cultivation, glucose was totally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytetracycline; Streptomyces rimosus; Bioreactor; Fed-batch cultivation; Feed-back control.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500676

Biofiltration of a styrene/acetone vapor mixture in two reactor types under conditions of styrene overloading
Provedor de dados: 52
Autores: Zapotocky,Lubos; Halecky,Martin; Vanek,Tomas; Paca,Jan; Kozliak,Evguenii; Jones,Kim; Soccol,Carlos Ricardo.
This aim of study was to compare the performance of a biofilter (BF) and trickle bed reactor (TBR) under increased styrene loading with a constant acetone load, 2 gc/m3/h. At styrene loading rates up to 30 gc/m3/h, the BF showed higher styrene removal than TBR. However, the BF efficiency started to drop beyond this threshold loading and could never reach steady state, whereas the TBR continued to yield a 50% styrene removal. The acetone removal remained constant (93-98%) in both the reactors at any styrene loading. Once the overloading was lifted, the BF recovered within 26 min, whereas the TBR efficiency bounced back only to 95%, gradually returning to complete removal only in 10 h.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofiltration; Styrene; Acetone; Vapor mixtures; Two reactors.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500782

Determination of the Effect of the Elimination Diet Applied for Overweight and Obese People with Food Intolerance on Body Composition and Biochemical Parameters
Provedor de dados: 52
Autores: Gubur,Sema.
ABSTRACT Food Intolerance, is a reaction against food, but not immunological manner, and may be confused with real food allergies. In this study, effects of special weight-loss diet together with an elimination diet on body composition and biochemical parameters of overweight and obese people who were diagnosed with food intolerance were investigated. The study group consists of 20 patients in total who were followed-up and treated in Yorktest Turkey Laboratory; who were diagnosed with food intolerance, and whose BMI was &gt; 26kg/m2. Bloodletting for these patients was executed with Lancet from their fingertips, and the blood drawn from these patients was assessed via enzyme-linked immunosorbent assay (ELİSA) method, and food reactions of patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food intolerance; Obesity; IgG; Elimination diet; Yeast.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100314

Effect of a phosphonium salt grafted on polymers on cucumber germination and initial growth
Provedor de dados: 52
Autores: Popa,Adriana; Crisan,Manulela; Visa,Aurelia; Ilia,Gheorghe.
The aims of this study were the synthesis, characterization and the testing of vinyldiphenylphosphonium salt phytotoxicity. Phytotoxicity of the synthesised salt was tested on Cucumis sativus L. seed germination and early seedling growth, at five different concentrations, using a standardized toxicity test. Endpoints included the determination of some seedling parameters: root and shoot length, root and shoot biomass, root and shoot dry weight ratio and final germination percentage. The vinyldiphenylphosphonium salt showed moderate to strong root length and root biomass inhibition, which increases progressively with the increasd of the concentration level.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vinyldiphenylphosphonium salt; Phytotoxicity; Cucumis sativus L.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100014

Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification
Provedor de dados: 52
Autores: Miranda,Vitor Fernandes Oliveira de; Martins,Vanderlei Geraldo; Furlan,Antonio; Bacci Jr.,Maurício.
The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase; DNA; Drosera; Fungi; Phylogeny.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018

Re-evaluation of manganese solubility as affected by soil sample preparation in the laboratory
Provedor de dados: 52
Autores: Andrade,Emilene; Miyazawa,Mário; Pavan,Marcos Antonio; Oliveira,Edson Lima de.
Laboratory experiments were conducted to re-evaluate the effects of drying and the time between drying and Mn analysis on soil Mn solubility using maize seedlings as test plant. Samples of five soil types were collected in the field, transferred to laboratory and submitted for the following treatments: dried in the shade at 25ºC and dried at 65ºC followed by Mn determination immediately and after 30 and 60 days. Ninety days later soil samples were rewetted at field capacity and maize seedlings were grown for 7 days. Evaluations included plant Mn content and soil Mn extracted with NH4OAc 1 mol L-1 pH 7. The lowest soil and plant Mn contents were found in soil samples dried in the shade at 25ºC. Drying soil sample at 65ºC and increasing the time between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronutrient; Transition metal; Soil analysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500017

PET and PET/CT imaging for the earliest detection and treatment of colorectal carcinoma
Provedor de dados: 52
Autores: Carter,Kevin; Kotlyarov,Eduard.
Approximately 150,000 new cases of colorectal cancer are diagnosed each year with the life time risk of developing colon caner in developed nations being 4.6% in men and 3.2% in women. Screening patients is essential early detection of colon carcinoma to aid in complete resection. Unfortunately current screening methods carry with them poor patient compliance. PET and PET/CT may be a significant part of this screening solution. The authors reviewed and analyzed the English language articles and case reports identified on Medline during the last 10 years. PET and PET/CT results for colorectal carcinoma were tabulated and presented for the fifth Scientific Meeting of the Brazilian Society of Nuclear Biosciences. Though most studies have been retrospective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PET and PET/CT; Colorectal cancer; Diagnostic.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700025

Induced immune response of Escherichia coli BL21 expressing recombinant MSP1a and MSP1b proteins of Anaplasma marginale
Provedor de dados: 52
Autores: Tamekuni,Katia; Vidotto,Marilda Carlos; Felix,Samuel Rodrigues; Igarashi,Michelle; Garcia,João Luis; Coelho,Adriana Letícia; Gasparini,Marcela Ribeiro; Marana,Elizabete Regina Marangoni; Silva,Sérgio Silva da; Vidotto,Odilon.
This work aims to evaluate the potential of immunization with E. coli BL21 expressing the recombinant rMSP1a and rMSP1b proteins of Anaplasma marginale. E. coli BL21 was transformed with recombinant plasmids pET102/msp1α and pET101/msp1β, and rMSP1a and rMSP1b were expressed after induction by IPTG. BALB/c mice were vaccinated with formolized BL21/rMSP1a and BL21/rMSP1b, and the production in mice sera of whole IgG was determined by ELISA. The mice immunized with BL21/rMSP1a showed a better humoral response for whole IgG when compared to the mice immunized with BL21/rMSP1b; these mice exhibited a small response after the second vaccination. Sera of mice immunized with BL21/rMSP1a reacted via western blot with BL21 and rMSP1a, with molecular masses varying...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaplasma marginale; MSP1a; MSp1b; Vaccine; Mice; Cattle.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700016

Impact of Fungicide Residues on Polymerase Chain Reaction and on Yeast Metabolism
Provedor de dados: 52
Autores: Silva,Gildo Almeida da; Bernardi,Taís Letícia; Schaker,Patrícia Dayane Carvalho; Agustini,Bruna Carla; Mello,Loiva Maria de; Valente,Patrícia.
ABSTRACT The indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungicide; PCR inhibitors; Stuck fermentation; Taq polymerase inhibitors; Yeast growth.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100515

Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)
Provedor de dados: 52
Autores: Ranzani-Paiva,Maria José Tavares; Ishikawa,Carlos Massatoshi; Eiras,Augusta Cocuzza das; Silveira,Vivian Risaffi da.
A hundred adult of tilapia, Oreochromis niloticus, were inoculated intraperitoneally with 10(8) c.f.u. of Mycobacterium marinum. Other 100 speciemns were maintained without inoculation. Blood samples of six inoculated animals were collected at 01, 03, 07, 14, 21, 28, 35, 42, 49, 56 and 84 days after inoculation. Initially, six specimens were taken from the non inoculated group ("time zero"). The haematological parameters determined were: haematocrit (Hct), haemoglobin concentration (Hgb), total erythrocytes count (RBC), hematological indexes (MCV, MCH and MCHC), thrombocyte count (Tr), leukocyte count (WBC) and leukocyte differential count. There were no changes in Hct, Hgb, MCH and MCHC. However, the mean values for RBC and MCV decreased. The mean values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium marinum; Tilapia; Oreochromis niloticus; Hematology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600014

Bony fish bycatch in the southern Brazil pink shrimp (Farfantepenaeus brasiliensis and F. paulensis) fishery
Provedor de dados: 52
Autores: Vianna,Marcelo; Almeida,Tabajara.
Trawling for pink shrimp (Farfantepenaeus brasiliensis and F. paulensis) catches also large quantities of fish, mostly bony fish, which are discarded, as they have no commercial value. Their composition and abundance were studied in an area that corresponded to the southeastern coastline of Brazil. Ninety one species were registered. Incidental fishing affected mainly small individuals and occured throughout the year. The year round rate of bony fish to shrimp catches was 10,5:1,0. Three assemblages of bony fishes could be identified, influenced by the seasonal variation of the water masses, the predominant group being associated with the Coastal Water, another with the penetration of colder waters onto the platform (SACW) and a third group of a more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bycatch; Bony fishes; Demersal fish; Shrimp fisheries.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500014

Effects of catuaba cristal® on the testis of wistar rats
Provedor de dados: 52
Autores: Freitas,Karine Moura de; Costa,Kyvia Lugate; Campos,Pamela Kelly Araújo; Melo,Fabiana Cristina Silveira Alves de; Paula,Tarcízio Antônio Rego de; Matta,Sérgio Luis Pinto da.
The aim of this study was to evaluate the possible beneficial effects of Catuaba Cristal® (CC), an alcoholic drink made from wine and Erythroxylum catuaba Ar. Cam on testis. Wistar rats either received CC solution (n=8) or water (n=9). Results showed significant body weight reduction within the CC group, although, no weight changes were observed for liver, kidney, testis, epididymis, seminal vesicle and prostate. The volumetric proportion and volume of interstitial tissue and lymphatic space were reduced in the treated group. In the CC group, although the nuclear volume of Leydig cells (LC) decreased, the number of LC per testis increased. These results suggested that CC had no beneficial effect on spermatogenesis of Wistar rats.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aphrodisiac drink; Medicinal plants; Morphometry; Spermatogenesis; Testis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500007

Morphological and Biochemical Answer of the Wheat Seeds at Treatment with 2,4-Dinitrophenol and Potassium Iodate
Provedor de dados: 52
Autores: Todirascu-Ciornea,Elena; Drochioiu,Gabi; Stefanescu,Raluca; Axinte,Elena Violeta; Dumitru,Gabriela.
Oxidative stress can be regarded as an imbalance between the amount of reactive oxygen species (ROS) and the ability of a biological system to eliminate the toxic species and repair the resulting damages. Since the germinating seeds and the resulted seedlings are rich in enzymes, whereas the treatment with chemicals affects much the seed germination, producing also ROS, we evaluate here the influence of 2,4-dinitrophenol (DNP) and potassium iodate (KIO3) on wheat germination (Triticum aestivum L.) and seedlings growth. Germination rate, the masses and heights of the 7 day old seedlings, as well as the activity of some enzymes involved in the oxidative stress such as peroxidase, catalase and superoxide dismutase were measured seven days after the chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNP; KIO3; Oxidative stress; Wheat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100414

Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization
Provedor de dados: 52
Autores: Padilha,Giovana da Silva; Santana,José Carlos Curvelo; Alegre,Ranulfo Monte; Tambourgi,Elias Basile.
This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two tie lines. Metal ions, pH and temperature effects on enzyme activity were evaluated. Five milliliter of 7.5% olive oil emulsion with 2.5% gumarabic in 0.1M sodium phosphate buffer at pH 8.0 and 37ºC were used for the activity determinations. Results showed that crude stratum from B. cepacia was partitioned by PEG1500/phosphate ATPS at pH 6.0 or 8.0 for, which the partitioning coefficients were 108-and 209-folds. Lipase presented optimal activity conditions at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipase; Burkholderia cepacia; Aqueous two phase system; Partitioning; Characterization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100002

Achene dimorphism in Bidens pilosa L. (Asteraceae) as determined by germination test
Provedor de dados: 52
Autores: Amaral,Adriana; Takaki,Massanori.
The germination of achenes of Bidens pilosa L. was analyzed during the life span of the mother plant. Decrease in the range of achene size was monitored until the senescence of the mother plant when the shortest achenes were harvested. The analysis of the germination percentage confirmed the presence of two distinct classes, formerly defined as short and long ones. Because length can not be used for the separation of those classes, the morphological characteristics of the tegument, specially of the ornament must be used for separation of the achenes with verrucose tegument (formerly named as short achenes) and achenes without ornament of the tegument (formerly named as long achenes). The achenes with verrucose tegument presented dormancy and light...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bidens pilosa; Seed dimorphism; Phytochrome; Seed germination.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100002

Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
Provedor de dados: 52
Autores: Palit,Soumen; Banerjee,Rintu.
The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus circulans; Solid state fermentation; Alpha- amylase; Extraction.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015

Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao
Provedor de dados: 52
Autores: Lopes,Francis Julio Fagundes; Queiroz,Marisa Vieira de; Lima,Juliana Oliveira; Silva,Viviane Aline Oliveira; Araújo,Elza Fernandes de.
The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the influence of the promoter used to express the gene marker were also analyzed. The addition of BamHI or NotI increased the number of transformants by 3-10-fold and 3-fold, respectively, over the control without added enzyme. The use of pre-linearized plasmid did not increase the transformation efficiency in comparison with the circular plasmid. However, the frequency of multi-copy transformants increased significantly. The transformation procedure here reported resulted in better production of protoplasts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moniliophthora perniciosa; Transformation; Restriction enzyme.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100004

Isolation and mycelial growth of Diehliomyces microsporus: effect of culture medium and incubation temperature
Provedor de dados: 52
Autores: Nascimento,José Soares do; Eira,Augusto Ferreira da.
The false truffle is one of the main problems in the production of the Agaricus brasiliensis in Brazil and the control of this fungal competitor has been rather difficult due to difficulties in the isolation and cultivation of this pathogen. This experiment was conducted in three stages, the first consisting of the isolation of Diehliomyces microsporus starting from portions of the fruiting body and through the ascospores suspension; second, D. microsporus cultivated in vitro at 15, 20, 25, 30 and 35ºC in six different culture media (CSDA, OCDA, PCDA, ODA, PDA, CDA); third, D. microsporus was inoculated on sterilized compost for formation of the fruiting body. The colony formation from tissue of D. microsporus starting from portions of fruiting body was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus brasiliensis; False truffle; Culinary-medicinal mushrooms; Culture media; Mushroom disease; Competitor.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400004

Effects of Chrysobalanus icaco on the labeling of blood constituents with technetium-99m and on the shape of the red blood cells
Provedor de dados: 52
Autores: Presta,Giuseppe Antonio; Santos-Filho,Sebastião David; Paoli,Severo de; Giani,Tania Santos; Maiworm,Adalgisa Ieda; Brandão-Neto,José; Medeiros,Aldo da Cunha; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Chrysobalanus icaco (abajeru; C.icaco) is recommended in the treatment of diabetes and other clinical disorders. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this study was to verify the effects of an abajeru extract on the labeling of blood constituents with 99mTc and on the shape of red blood cells (RBC). Blood samples(Wistar rats) were incubated with abajeru extract and the labeling of blood constituents with 99mTc and morphology of RBC were carried out. The results showed significant (P&lt;0.05) alteration of labeling of blood constituents with 99mTc and the morphometry (perimeter/area ratio) of the RBC in presence of the extract. These data suggest that this abajeru extract could alter the labeling of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red blood cells; Technetium-99m; Morphometry; Chrysobalanus icaco.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600018

Effects of the Photovoltaic Distributed Generation on Electricity Distribution System Voltage - Updated Review
Provedor de dados: 52
Autores: Melo Júnior,Celso Fabrício; Waenga,Aline F. Cordeiro; Pinto,Dayana A. Ferreira.
ABSTRACT Concerns about the environment and a growing demand for electricity have led to the intensive search for renewable energy sources. Photovoltaic solar energy, in the form of distributed generation, has shown a very high growth rate to an extent that in some regions it will be adopted on a large scale, thus being responsible for the supply of a significant portion of the load of this region. Some factors have favored the adoption of distributed generation: the drop in the price of solar panels and inverters, as well as its easy installation and maintenance. However, the large-scale adoption of solar distributed generation brings new challenges to the power distribution system. Network voltage control is an example. In conventional systems, voltage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic distributed generation; Voltage ripple; Flicker; Power quality.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200206

Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate
Provedor de dados: 52
Autores: Braunig,Patricia; Rosa,Rafael Diego da; Seibert,Caroline Heidrich; Borsa,Mariana; Stoco,Patricia Hermes; Grisard,Edmundo Carlos; Pinto,Aguinaldo Roberto.
In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: WSSV; VP28; Recombinant protein; Penaeid shrimp.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200023

Bioactive amines in Brazilian wines: types, levels and correlation with physico-chemical parameters
Provedor de dados: 52
Autores: Souza,Solange C.; Theodoro,Karine H.; Souza,Élson R.; Motta,Silvana da; Glória,Maria Beatriz Abreu.
The levels of ten bioactive amines and the physico-chemical characteristics of Cabernet Sauvignon, Cabernet Franc and Merlot wines from Bento Gonçalves, RS, Brazil, vintage of 1999 were investigated. The physico-chemical characteristics varied significantly: pH from 3.80 to 4.07, total acidity from 67.7 to 85.3 meq/L, alcohol content from 11.45 to 12.46 mL/100 mL and total SO2 from 9.6 to 102 mg/L. Six amines were detected in every sample - spermidine, putrescine, histamine, tyramine, serotonine and phenylethylamine. Total amine levels ranged from 2.03 to 7.60 mg/L. Putrescine was the prevalent amine, contributing with 20 to 66% of total levels. The amine profile and total levels were affected to a greater extent by vinification practices compared to grape...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red wine; Amines; Histamine; Tyramine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100009

Inheritance of resistance to races 69 and 453 of Colletotrichum lindemuthianum in the common bean
Provedor de dados: 52
Autores: Poletine,Juliana P.; Gonçalves-Vidigal,M.C.; Vidigal Filho,Pedro S.; Scapim,Carlos Alberto; Silvério,Lucas; Thomazella,Cláudia.
The cultivars, AB 136 and G 2333 both resistant to Colletotrichum lindemuthianum races 69 and 453, were crossed with the cultivars Michelite and Perry Marrow (susceptible to both races), with Dark Red Kidney and Cornell 49242 (resistant to both races) and F1 and F2 generations were obtained. Plants were inoculated using a spore suspension at 1.2 x 10(6) concentration. The reaction of F1 and F2 populations showed that Dark Red Kidney, Cornell 49242 and AB 136 cultivars had the dominant genes A (Co-1), Are (Co-2) and Co-6, respectively, was conferring resistance to races 69 and 453. The segregation data obtained from F2 populations indicated that G 2333 carried two dominant resistance genes Co-5 gene and another one Co-7 for 69 and 453 races. The dominant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Colletotrichum lindemuthianum; Anthracnose; Resistance.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500006

Tolerance to aluminum toxicity by tropical leguminous plants used as cover crops
Provedor de dados: 52
Autores: Meda,Anderson Rotter; Furlani,Pedro Roberto.
The objectives of this work were to compare Al tolerance among 17 species of tropical leguminous plants and to evaluate the most appropriate plant characteristic and Al concentration in nutrient solution for tolerance assessment. In addition, three soybean cultivars were included for comparison purposes. There was a great difference among the 17 plant materials tested, when compared by relative root elongation and critical Al activity to reduce 50% of root net elongation. Based on these parameters and on the comparison of two tropical maize genotypes differing in Al tolerance, the following classification was established: highly tolerant, for Mucuna nivea, M. deeringiana, M. aterrima Vigna unguiculata cv. BR 17 and Lablab purpureus cv. Rongai; tolerant for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrient solution culture; Root growth; Dry matter yields; Al speciation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200019

Seed morphology and post-seminal development of Tillandsia L. (Bromeliaceae) from the "Campos Gerais", Paraná, Southern Brazil
Provedor de dados: 52
Autores: Scatena,Vera Lucia; Segecin,Simone; Coan,Alessandra Ike.
This work studied the morphology and post-seminal development of seeds of Tillandsia gardneri Lindl., T. streptocarpa Baker, and T. stricta Sol. ex Sims (Bromeliaceae) from the "Campos Gerais", State of Paraná, Southern Brazil. The seeds (ca. 2.0 mm long) presented plumose appendages at their micropylar end, an important structure in the taxonomy of the family since it represented an elaborate apparatus allowing effective wind-dispersal. Seeds germinated after circa twenty days. The haustorial cotyledon, responsible for the seedling nutrition, appeared first during the germination process. No cataphyll or primary roots developed. A small tank came along in the seedling, formed by the base of the sheath of the first leaf, which overlapped that of the second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tillandsia; Seed morphology; Post-seminal development.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700012

Population structure and somatic indexes of Hypostomus cf. ancistroides (Siluriformes, Loricariidae) collected from the Bonito river, Ivaí river basin, Turvo, Paraná
Provedor de dados: 52
Autores: Viana,Douglas; Wollf,Luciano Lazzarini; Zaleski,Tânia; Romão,Silvia; Bertoldi,Gustavo; Donatti,Lucélia.
This study aimed to provide information about the population structure and somatic index of Hypostomus cf. ancistroides collected from the Bonito river, located in the Ivaí river basin. The length-weight relationship was isometric for both the sexes. The length structure analysis showed that the larger individuals (from 18.1cm to 27.0cm in length) predominated, and the lowest abundances occurred at the size extremes (9.1-12cm and 27.1-30.0cm). The reproduction period occured between October and January for the females and between November and January for the males. The liver somatic index cannot be used as an indicator of the reproduction period in either of the sexes, due to no correlation between the liver somatic index and the gonad somatic index. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonad somatic index; Liver somatic index.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300008

Leaf Structure and Taxonomy of Petunia and Calibrachoa (Solanaceae)
Provedor de dados: 52
Autores: Reis,Claudia dos; Sajo,Maria das Graças; Stehmann,João Renato.
We studied the leaf anatomy of sixteen species of Calibrachoa and eight species of Petunia. In Calibrachoa leaves, the vascular bundles sheath (endodermis) was formed by parenchymatous developed cells, different from those of the mesophyll. In Petunia, this sheath did not show a marked morphological differentiation. The Calibrachoa leaves could be separated according to the type of leaf margins, the distribution of the stomata on leaf surfaces, the organization of the mesophyll and the morphology of the trichomes. Based on these results, an indented dichotomous identification key was elaborated for the species of the genus Calibrachoa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanaceae; Calibrachoa; Petunia; Leaf anatomy; Taxonomy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100010

An improvement of calcium determination technique in the shell of molluscs
Provedor de dados: 52
Autores: Soído,Cristiane; Vasconcellos,Maurício Carvalho; Diniz,Antônia Gonçalves; Pinheiro,Jairo.
The complexometric method is usually applied to quantitative calcium determination in different materials; however the application of this method to calcium determination in molluscs shells infers significant interferences to the results. The snail Bradybaena similaris, a terrestrial gastropod, was used as experimental model to the improvement of this method. The shells were calcinated and dissolved in nitric acid, the hydrogen peroxide was also used to clarify the medium after the acid addition. The calcination procedure and the use of nitric acid reduced the significantly the interferences, allowing a major degree of destruction of the organic substances of the shell. The improvement of the calcium determination technique usually employed showed calcium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snail; Bradybaena similaris; Shell; Calcium determination technique.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100012

Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation
Provedor de dados: 52
Autores: Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud.
ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100401

Simple models for the continuous aerobic biodegradation of phenol in a packed bed reactor
Provedor de dados: 52
Autores: Gerrard,Andrew Mark; Páca Júnior,Jan; Kostecková,Alena; Páca,Jan; Stiborová,Marie; Soccol,Carlos Ricardo.
This paper proposes the use of a preliminary, phenol removal step to reduce peak loads arriving at a conventional effluent plant. A packed bed reactor (PBR) using polyurethane foam, porous glass and also cocoa fibres as the inert support material was used. Experiments have been carried out where the flow-rates, plus inlet and outlet phenol concentrations were measured. A simple, plug-flow model is proposed to represent the results. Zero, first order, Monod and inhibited kinetics rate equations were evaluated. It was found that the Monod model gave the best fit to the experimental data and allowed linear graphs to be plotted. The Monod saturation constant, K, is approximately 50 g m-3, and ka is around 900 s-1.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Phenol; Aerobic reactor; Models.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500018

In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe
Provedor de dados: 52
Autores: Delmail,David; Labrousse,Pascal; Lesouëf,Jean-Yves; Le Hir,François; Autret,Jean-Luc; Botineau,Michel.
Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Normania; Endemic species; Micropropagation; 6‑benzylaminopurine; Callus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009

Pollination ecology of Swartzia apetala Raddi var. apetala (leguminosae-papilionoideae)
Provedor de dados: 52
Autores: Moço,Maria Cecília de Chiara; Pinheiro,Maria Célia Bezerra.
The pollination ecology of Swartzia apetala Raddi var. apetala was studied in the restinga of Maricá, State of Rio de Janeiro, Brazil. S. apetala var. apetala is a shrub with asynchronic annual blooming, between the months of November and April (hot-rainy season). Each plant can exhibit more than one blooming episode in this period. Their flowers are hermaphrodites, apetalous, heterantherous, odoriferous, and the pollen represents the only reward offered to visitors. It is pollinized by species of Centris and Xylocopa, solitary and polyletic bees, which collect the pollen by means of vibratory movements. Other bees such as Apis mellifera, Pseudaugochloropsis graminea and Trigona spinipes are only pollen-thieves. In this variety of Swartzia vibrating is an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leguminosae; Papilionoideae; Swartzia; Buzz-pollination; Pollen-flower.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400006

Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts
Provedor de dados: 52
Autores: Takahashi,Elizabete Keiko; Kobayashi,Adilson Kenji; Vieira,Luiz Gonzaga Esteves.
The objective of this study was to examine the effect of two culture systems, liquid medium associated to floating membranes and solid medium, both supplemented with different concentrations of 2,4-D, in the induction of somatic embryogenesis of cassava (Manihot esculenta Crantz). Only 28% of the young leaf lobes (with 9 µM 2,4-D) were induced to form organized embryogenic structures (OES) with membrane rafts, compared to 50% of the explants presenting this type of tissue in solid medium with 36 µM of 2,4-D. Despite the lower response observed in liquid medium with membrane, the amount of OES/explant in all 2,4-D concentrations was higher than solid medium. Based on the results and considering the high cost of the membrane rafts, this system was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Somatic embryogenesis; Membrane rafts; Organized embryogenic structures (OES).
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100005

Seed reserves partition and light compensation point of mahogany (Swietenia macrophylla King) seedlings growth under low photosynthetic active radiation
Provedor de dados: 52
Autores: Lemos Filho,José Pires de; Duarte,Ricardo Jenner.
The aim of this study was to verify the dry mass partition of the seed reserves during the initial growth of Swietenia macrophylla seedlings, in the dark, and at low levels of photosynthetically active radiation: 0.125; 3.12 and 52 µ mol.m-2.s-1. After 50 days, the dry mass of the seed reserves did not differ with treatments, but the total dry mass and leaf area were higher in the seedlings under higher light treatment. No difference in root/shoot ratio was observed between treatments, but the leaf area ratio was lower at higher light. Only the seedlings grown at 52 µ mol.m-2.s-1 showed a positive dry mass increase in relation to the mobilized seed reserves. With the values of the net increase of the seedling mass, a linear equation was adjusted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swietenia macrophylla; Dry mass partition; Light compensation point; Shade adaptation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200006

Dispersion of Aedes aegypti (Linnaeus, 1762) and Aedes albopictus (Skuse, 1894) in the rural zone of north Paraná State
Provedor de dados: 52
Autores: Lopes,José; Martins,Emerson Augusto Castilho; Oliveira,Osmar de; Oliveira,Valdecir de; Oliveira Neto,Bertolino Pedro de; Oliveira,José Eduardo de.
Seventy-two tyres were placed in four transects (Northern, Southern, Eastern and Western), every 5 km, from the Londrina city limits to the rural areas, to verify the dispersion of Aedes aegypti and Ae. albopictus vectors. Mosquito larvae were collected fortnightly from August 1998 to August 1999. Data were organized according to the average number of larvae collected for each species in each collection site. A total of 62,517 mosquito larvae were collected and distributed into the following species: Aedes albopictus (21.71%); Ae. aegypti (5.54%); Ae. terrens (0.53%); Ae. fluviatilis (0.36%); Anopheles argyritarsi (0.01%); Culex quinquefasciatus (48.37%); Cx. mollis (8.88%); Cx. eduardoi (8.65%); Cx. corniger (0.61%); Cx. bigoti (0.24%); Cx. grupo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes; Population dispersion; Dengue; Prevention and control; Breeding sites.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500009

Selection of microorganisms degrading S-Metolachlor herbicide
Provedor de dados: 52
Autores: Martins,Paula Fabiane; Martinez,Camila Ortiz; Carvalho,Giselle de; Carneiro,Paulo Irajara Borba; Azevedo,Ricardo Antunes; Pileggi,Sônia Alvim Veiga; Melo,Itamar Soares de; Pileggi,Marcos.
The aim of this work was to study herbicide degradation through selected microorganisms from humus and soil subjected to different plantation systems. The following bacterial species were identified: Klebsiella pneumoniae pneumoniae GC s.B strain 1, Pseudomonas alcaligenes, Enterobacter aerogenes GC s.A and Klebsiella pneumoniae pneumoniae GC s.B strain 2. Growth studies yet suggested the possibility of a very long lag phase. Although, culture with the herbicide presented biofilm formation and there were color changes in the herbicide that could have interfered with the espectrophotometry readings. After 5 days of incubation at 35ºC, the difference in the concentration of herbicide was 14.42% on average and after 10 days, 35.01%.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioremediation; Biodegradation; S-Metolachlor; Herbicide.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100019

Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
Provedor de dados: 52
Autores: Klemz,Claudio; Salvo,Ligia Maria; Bastos Neto,Jayme da Cunha; Bainy,Afonso Celso Dias; Assis,Helena Cristina da Silva de.
Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome P450; Fish; Ancistrus multispinis; Biomarker.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015

Erratum: Smart Health Surveillance with Automated Database Using Android Mobile Device
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100912

Optimization of Fermentation Medium for Enhanced Glucansucrase and Glucan Production from Weissella confusa
Provedor de dados: 52
Autores: Shukla,Shraddha; Goyal,Arun.
A glucan producing Weissella confusa isolated from fermented cabbage, as identified earlier, was used for optimization of its fermentation medium. The effects of various macronutrients such as sucrose, glucose as cosubstrate, yeast extract, beef extract, peptone, sodium acetate, K2HPO4 and Tween 80 were studied on glucansucrase and glucan production from Weissella confusa. The medium used as control gave 6.0 U/ml enzyme activity and 34 mg/ml glucan concentration. Sucrose (5%), glucose as cosubstrate (5% for glucansucrase and 3% for glucan production), Tween 80 (0.1%), yeast extract (1.5%), Peptone (2.0%) and K2HPO4 (1.5%) were effective nutrients displaying higher glucansucrase and glucan production giving 18.2, 18.0, 7.0, 6.4, 6.2 and 6.4 U/ml enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Weissella confusa; Glucansucrase; Glucan; Tween 80.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600006

Novel Food Supplements Formulated With S pirulina To Meet Athletes’ Needs
Provedor de dados: 52
Autores: Carvalho,Lisiane Fernandes de; Moreira,Juliana Botelho; Oliveira,Mariana Souza; Costa,Jorge Alberto Vieira.
ABSTRACT The food, training, and health are crucial for a good performance in sports. Intense physical activity takes the athlete to maintain a very unstable balance between energy demand and consumption of nutrients. Spirulina microalga has a nutritional profile that renders it an ideal food supplement, because has high protein content, also contains vitamins, minerals, and pigments. In this context, the study aimed to develop, characterize and evaluate the stability of foods enhanced with Spirulina, which are intended for athletes. In this study, six different supplements were developed (electrolyte replenisher, muscle enhancer, and recovery supplement), without and with Spirulina. The electrolyte replenisher with Spirulina compared to the product...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Athletes; Microalgal biomass; Performance; Supplement.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100302

Antifeedant activity of Melia azedarach (L.) extracts to Diabrotica speciosa (Genn.)(Coleoptera: Chrysomelidae) beetles
Provedor de dados: 52
Autores: Ventura,Maurício Ursi; Ito,Márcio.
Studies were carried out to determine the antifeedant activity of extracts of leaves, stems, flowers and fruits of Melia azedarach (L.), the dosage activity responses to the most active extract and the effects of sunlight in the activity persistence to Diabrotica speciosa (Genn.) beetles. Extracts efficiency was determined by evaluating leaf consumption. Insect feeding was deterred by stems, fruits and flowers extracts. Flowers and fruits extracts were the most efficient. Stems extract was in an intermediate position between the two most efficient and the least one (leaf extracts). The most active extract (flowers) was sprayed at concentrations of 0, 1, 2, 3, 4, 5, 6 and 7 g/100 ml. Feeding of D. speciosa on common bean leaves extracts decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Leaf beetle; Phaeolus vulgaris (L.); Deterrence; Botanical insecticide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200011

Secure Deduplication for Cloud Storage Using Interactive Message-Locked Encryption with Convergent Encryption, To Reduce Storage Space
Provedor de dados: 52
Autores: Jayapandian,N; Md Zubair Rahman,A M J.
ABSTRACT The digital data stored in the cloud requires much space due to copy of the same data. It can be reduced by dedupilcation, eliminating the copy of the repeated data in the cloud provided services. Identifying common checkoff data both files storing them only once. Deduplication can yield cost savings by increasing the utility of a given amount of storage. Unfortunately, deduplication has many security problems so more than one encryption is required to authenticate data. We have developed a solution that provides both data security and space efficiency in server storage and distributed content checksum storage systems. Here we adopt a method called interactive Message-Locked Encryption with Convergent Encryption (iMLEwCE). In this iMLEwCE the data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Data compression; Database systems; Cloud computing; Encryption; Secure storage.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100402

Investigation the natural radioactivity in local and imported chemical fertilizers
Provedor de dados: 52
Autores: Hussain,Raad Obid; Hussain,Hayder Hamza.
In order to assess the radioactive level in the environment, the specific activities of 238U, 232Th and 40K were measured by using a gamma ray spectrometer based on a NaI(Tl) detector. The results showed that the specific activities of 238U, 232Th and 40K were 12.846-88.585, 1.304-26.656 and 11.598-2275.896 Bq/kg respectively. The radium equivalent activity was also calculated which varied from 0.893 to 175.244 Bq/kg. The radium equivalent average values were lower than the world average. In general, the highest level of radium equivalent value was in leafy type NPK fertilizers. The urea type had no radionuclide. The specific activity and absorbed dose rate at 1m above the ground surface (nGy/h) after NPK fertilizers agricultural applications were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural radioactivity; Gamma-spectrometry; Fertilizers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400018

Heterosis in soybeans for seed yield components and associated traits
Provedor de dados: 52
Autores: Pandini,Fábio; Vello,Natal Antonio; Lopes,Ângela Celis de Almeida.
The objective of this research was to quantify heterosis of agronomic traits and evaluate direct and indirect correlations among seed yield and other traits. A diallel involving six parents was grown in two localities. Seed yield (PG), one-hundred seed weight (PCS), number of pods per plant (NV), weight of aerial part of the plant (PPA), harvest index (IC) and number of seeds per pod (NGV) were evaluated. Positive values of heterosis were detected for all traits. Estimates of heterosis components were significative for most traits, showing effects of aditivity and dominance. The specific heterosis was more important than the variety heterosis, mainly in the locality Anhembi. MTBR-95-123800 presented the best potential per se and as parent in crosses, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Indirect selection; Correlations among traits; Path analysis; Glycine max.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600001

Physicochemical characterization of saccharides powder obtained from yacon roots (Smallanthus sonchifolius) by membrane technology
Provedor de dados: 52
Autores: Alles,Maria Julia Ledur; Tessaro,Isabel Cristina; Noreña,Caciano Pelayo Zapata.
Fructooligosaccharides (FOS) are food ingredients applied due to combination of prebiotic benefits and interesting technological properties, being used as a sugar substitute. The knowledge of their physicochemical characteristics is important for predicting the stability of the food products where they are added. For this reason, a saccharides powder extracted from yacon (Smallanthus sonchifolius) roots by membrane technology and freeze-dried was investigated. Color, water activity (aw), microstructure by Scanning Electron Microscopy coupled to Energy- dispersive X-ray Spectroscopy (SEM-EDS) and crystallinity by X-ray diffraction were evaluated. The obtained product, containing 19.75% FOS, 36.66% glucose and 43.59% fructose (w/w) was a whitish granulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yacon; Fructooligosaccharides; Microstructure; Cristallinity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600019

International and Brazilian Versions of WHOQOL-DIS: (in)adequacy to its Underpinnings
Provedor de dados: 52
Autores: Cantorani,José Roberto Herrera; Pedroso,Bruno; Vargas,Leandro Martinez; Picinin,Claudia Tania; Pilatti,Luiz Alberto; Gutierrez,Gustavo Luis.
Abstract: The present article aims to analyze the WHOQOL-DIS (World Health Organization Quality of Life - Disabilities) structure to verify this instrument’s adequacy to the theoretical principles underlying its construction. The methodology consists in an analysis of the WHOQOL-DIS underlying theory, prompting questions about the adequacies of this theoretical model. The procedure is complemented by a syntax analysis in order to verify the relevant questions for the score calculation. The obtained results show that the subjects “participation” and “autonomy” are not objectively present in the international version, although they are considered in the theories as justifying elements for the instrument’s creation. In the Brazilian version, there are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality of life; Disabled persons; Disability evaluation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100313

Thermostabilisation of human serum butyrylcholinesterase for detection of its inhibitors in water and biological fluids
Provedor de dados: 52
Autores: Jaganathan,Lakshmanan; Boopathy,Rathanam.
The ability of gelatine-trehalose to convert the normally fragile, dry human serum BChE into a thermostable enzyme and its use in the detection of cholinesterase inhibitors in water and biological fluids is described. Gelatine or trehalose alone is unable to protect the dry enzyme against exposure to high temperature, while a combination of gelatine and trehalose were able to protect the enzyme activity against prolonged exposure to temperature as high as +50°C. A method for rapid, simple and inexpensive means of screening for cholinesterase inhibitors such as carbamates and organophosphates in water, vegetables and human blood has been developed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butyrylcholinesterase; Carbamates; Cholinesterase; Gelatine; Organophosphorus; Trehalose.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300012

Effects of Replacement of Fishmeal with other Alternative Plant Sources in the Feed on Proximate Composition of Muscle, Liver and Ovary in Tilapia (Oreochromis nioloticus)
Provedor de dados: 52
Autores: Al-Ghanim,Khalid; Al-Thobaiti,Ahmed; Al-Balawi,Humoud F. Alkahem; Ahmed,Zubair; Mahboob,Shahid.
ABSTRACT The major objective of this experiment was to assess the effect of alternate plant protein sources as a replacement for fish meal in feed on the proximate composition of muscle, liver and tissue in Oreochromis niloticus. O. niloticus of average size (average Weight 45.00±1.25 g, total length 13.28±1.42 cm) were stocked in 100 L glass aquarium. Fish were fed with three experimental feeds (A, B and C) and reference commercial feed (D) for 16 weeks. Feed A, B and C was prepared from four different plant sources and fish meal (40 % crude protein). Fish were fed at the rate 3 % of body weight daily. It has been observed that tilapia fed with feed B in which 20 % fish meal was replaced compared to feed C with other plant sources of protein, had shown...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feed; Plant sources; Muscle; Liver; Ovary.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100420

Biochemical and morphological changes during the growth kinetics of Araucaria angustifolia suspension cultures
Provedor de dados: 52
Autores: Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Maraschin,Marcelo; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 µM 2,4-dichlorophenoxyacetic acid, 1 µM 6-benzylaminopurine and 1 µM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive reduction during culture time. For both the embryogenic cultures a preferential uptake of glucose in the late stages of cell growth kinetics was observed. The extracellular protein content was similar for both the embryogenic cultures. Acetocarmine and Evan's blue double stain showed major differences for early somatic embryo organisation, in which only the embryogenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucariaceae; Glucose; PH changes; Protein; Somatic embryogenesis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300001

Anti-Candida activity and chemical composition of Cinnamomum zeylanicum blume essential oil
Provedor de dados: 52
Autores: Castro,Ricardo Dias de; Lima,Edeltrudes Oliveira.
The purpose of this study was to identify the anti-Candida activity and chemical composition of the essential oil (EO) of Cinnamomum zeylanicum (cinnamon). For this, tests were conducted to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) and the action of C. zeylanicum EO on fungal cell wall of Candida albicans, C. tropicalis and C. krusei strains. The composition of the was analysed by gas chromatography with mass spectrometry. Significant antifungal activity of the EO was observed on the strains tested, with 87.5% and 62.5% of them sensitive, respectively at a MIC of 312.5 µg/mL and MFC of 2500 µg/mL. In the presence of sorbitol, the MIC was 625 µg/mL against all the strains, showing a possible action of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidiasis; Cinnamomum zeylanicum; Essential oils.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500005

Nanoparticle-based drug delivery systems: promising approaches against infections
Provedor de dados: 52
Autores: Ranghar,Shweta; Sirohi,Parul; Verma,Pritam; Agarwal,Vishnu.
Despite the fact that many new drugs and technologies have been developed to combat the infectious diseases, these have continued to be global health challenges. The use of conventional antimicrobial agents against these infections is always associated with problems such as the development of multiple drug resistance and adverse side effects. In addition, the inefficient traditional drug delivery system results in inadequate therapeutic index, low bioavailability of drugs and many other limitations. In this regard, antimicrobial nanoparticles and nanosized drug delivery carriers have emerged as potent effective agents against the infections. Nanoparticles have unique properties owing to their ultra small and controllable size such as high surface area,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial Nanoparticles; Drug Resistance; Drug Delivery System; Infection; Metal and Metal Oxide.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200008

Alterations in proteins and amino acids of the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense in response to industrial wastewater pollution
Provedor de dados: 52
Autores: El-Sheekh,Mostafa Mohamed; El-Otify,Ahmed Mohamed; Saber,Hani.
The effect of industrial wastewater on the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense was investigated. The data showed that P. limnetica was more sensitive to pollution than A. wisconsinense. The treatments with different levels of wastewater exerted pronounced reductions in protein and amino acids content. SDS-PAGE analysis revealed that the cyanobacteria grown in the industrial wastewater showed induction in the synthesis of certain polypeptides and repression of others. The treatments of P. limnetica with wastewater stimulated the appearance of six protein bands with molecular masses of 28, 30, 31, 32, 58 and 97 kDa. The same treatments caused the disappearance of 20, 38 and 56 kDa. The structural protein pattern of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Anabaena; Electrophoretic patterns; Nile cyanobacteria; Protein; Pseudanabaena; Wastewater.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400022

Root anatomy of nine orchidaceae species
Provedor de dados: 52
Autores: Oliveira,Virginia del Carmem; Sajo,Maria das Graças.
Studies on the root anatomy of nine Orchidaceae species presented a multisseriated velamen, a parenchymatous cortex and a definided endodermis, in all the studied species (Catasetum fimbriatum Lindl., Dichaea bryophila Rchb. f., Encyclia calamara(Lindl.) Pabst, Epidendrum campestre Lindl., Epidendrum secundum Jacq., Miltonia flavescens Lindl., Pleurothallis smithiana Lindl., Stanhopea lietzei (Regel) Schltr. and Vanda tricolor Lindl.) Structural characters, which could be considered root adaptations to an epiphytic habit, were also common for all species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Root anatomy; Orchidaceae; Ephiphytism; Structural characters.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400005

Simplified Methodology for Temperature Calculation of Operation and Photovoltaic Modules Yield in Non-Standardized Environmental Conditions
Provedor de dados: 52
Autores: Souza,Muryllo Amalio de; Souza,Jean Przybysz de; Pereima,Antonio Tadeu.
ABSTRACT This work proposes a simplified methodology to obtain the needed data to determine and investigate the variation of photovoltaic modules performance under non-standardized environmental conditions - Standard Test Conditions and Nominal Operating Cell Temperature. This methodology uses a previously developed mathematical model in association with environmental parameters as wind speed, air temperature and irradiance in different cities, located in different regions in Brazil. These data are obtained from both SWERA project and the National Institute of Meteorology of Brazil websites, both of them with free access on the internet. The result of this methodology is the operating temperature of a commercial polycrystalline module of 330 Wp and 1.95...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solar Energy; Photovoltaic module; Efficiency; Wind speed; Air temperature.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200204

Cytotaxonomic and evolutionary considerations about karyotipic data of fishes from the Iguaçu River Basin in South of Brazil
Provedor de dados: 52
Autores: Kantek,Daniel Luis Zanella; Cipriano,Roger Raupp; Abilhoa,Vinícius; Artoni,Roberto Ferreira; Cestari,Marta Margarete.
The cytogenetic data available in the literature about the ichthyofauna of the Iguaçu River basin were analyzed in this review. The ichthyofauna was characterized by the high level of endemism and by the low diversity of species. Twenty-four of the eighty-one species were already karyotyped; six Characiformes, fourteen Siluriformes and four Perciformes. The chromosomal data showed the taxonomic and systematic complexity of the groups. Hypothesis related to the evolution of some Characiformes and Siluriformes groups from the Iguaçu River are proposed, as well as the utilization of karyotypic data for cytotaxonomy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neotropical region; Fish cytogenetics; Chromosome formulae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500007

Influence of light intensity on growth and physiological characteristics of common sage (Salvia officinalis L.)
Provedor de dados: 52
Autores: Zervoudakis,George; Salahas,George; Kaspiris,George; Konstantopoulou,Eleni.
The aim of this work was to investigate the effects of four different light intensities on the growth characteristics, physiological parameters and leaf photosynthetic pigments of Salvia officinalis L. The plant's dry mass, number of the leaves and physiological parameters indicated a strong positive correlation with the light intensity. On the other hand, the plant's height and leaf photosynthetic pigments were increased at low light treated plants. These results suggest that the aromatic herb Salvia officinalis L. is adaptable to different light environments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Photosynthetic pigments; Physiological parameters; Salvia officinalis; Solar radiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100011

Photosynthetic response of soybean to twospotted spider mite (Acari: Tetranychydae) injury
Provedor de dados: 52
Autores: Bueno,Adeney de Freitas; Bueno,Regiane Cristina Oliveira de Freitas; Nabity,Paul David; Higley,Leon George; Fernandes,Odair Aparecido.
The twospotted spider mite Tetranychus urticae Koch is a common pest on soybean plants. To clarify plant-arthropod interaction on mite-soybean system, leaf fluorescence, photosynthetic responses to variable carbon dioxide levels, and chlorophyll content were evaluated. Significant photosynthetic rate reduction was observed due to stomatal limitation. Stomatal closure was the major plant physiological response. As a consequence, there was reduction in photosynthetic rates. Surprisingly, plants did not show chlorophyll content reduction associated with photosynthetic impairment. No differences in fluorescence data indicate that T. urticae injury did not impair the function of light harvesting and photoelectron transport. These results showed that T. urticae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetranychus urticae; Insect-plant relationship; Fluorescence; Gas exchange; Photosynthesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400005

Changes in nutrient loads (N, P and Si) in the São Francisco estuary after the construction of dams
Provedor de dados: 52
Autores: Medeiros,Paulo Ricardo Petter; Knoppers,Bastiaan Adriaan; Cavalcante,Geórgenes Hilário; Souza,Weber Friederichs Landim de.
The aim of this study was to investigate the post-dam conditions of the loads and yields of dissolved inorganic nitrogen (DIN), orthophosphate (DIP, silicate (DSi) and total phosphorous (TP) in the Lower São Francisco river estuary (NE-Brazil) after the river was regulated to a constant flow by the dams. Loads and yields of monthly measurements performed from November 2000 to March 2002 at a gauging station downstream of the dams (80 km from the coast) showed 4.1 x 10³ t/yr and 0.006 t/km² /yr of DIN, 0.2 x 10³ t/yr and 0.002 t/km² /yr of DIP, and 448 x 10³ t/yr and 0.71 t/km² /yr of DSi, respectively. Over the last 15 years, DIN loads reduced by 94 % and DSi by 31%. The river turned into an oligotrophic system with primary production limited by nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrients; Fluvial loads; Dam impacts; Estuary; São Francisco River (NE-Brazil).
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200022

Heavy Metals and 2,4-Dinitrophenol Impact on Some Physiological and Biochemical Parameters in Capsicum Species
Provedor de dados: 52
Autores: Todirascu-Ciornea,Elena; Dumitru,Gabriela.
Abstract The accumulation of heavy metals and of pesticides in the soil have a negative impact on Capsicum chinense var. Aji Mochero and Capsicum baccatum var. pendulum Bishop's Crown species, the present work highlighting the exerted influence on the superoxid-dismutase activity, catalase and peroxidase - as enzymes implied in the removal of H2O2, of O2- radical and of ion HO- that have devastating effects on the vegetable cell, on the Krebs cycle’s dehydrogenases - as main way to produce energy, respectively of the chlorophyll a, b and carotenes - as photoassimilatory pigments, but also as biologic antioxidants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dinitrophenol; Heavy metals; Capsicum; Oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100413

Resistance of oxidative stress in biofilm and planktonic cells
Provedor de dados: 52
Autores: Jakubowski,Witold; Walkowiak,Bogdan.
This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H2O2, tBOOH, menadione, SIN-1 or peroxynitrite) in a wide range of final concentrations, from 0.5 to 10mM. Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reactive oxygen species; Biofilm; Bacterial colonization; Planktonic cells; Implant.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200300

Biocompatibility, osteointegration, osteoconduction, and biodegradation of a hydroxyapatite-polyhydroxybutyrate composite
Provedor de dados: 52
Autores: Reis,Emily Correna Carlo; Borges,Andréa Pacheco Batista; Fonseca,Cláudio César; Martinez,Mastoby Miguel Martinez; Eleotério,Renato Barros; Morato,Gláucia Oliveira; Oliveira,Paulo Miranda.
In this work, biocompatibility, osteointegration, osteoconductivity, and biodegradation of a hydroxyapatite polyhydroxybutyrate new composite were evaluated. The composite was implanted in rabbits' bone defects and clinical, radiographic, histological, and histomorphometric data of these animals were compared with those of unfilled defects on the days 8th, 45th, and 90th after surgery. No significant differences existed between the groups for the evaluated clinical parameters. Radiographs showed bone-composite direct contact. Bone formed within the defect, interface and inside the composite. Significant differences were found between the bone and connective tissues percentage within the defect at all dates and at the interface on the 45th day, bone tissue...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterials; Bone; Biocompatibility; Biodegradation; Hydroxyapatite-polyhydroxybutyrate composite.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400010

Modificações dos componentes de parede celular do melão 'Orange Flesh' submetido a tratamento pós-colheita com cálcio
Provedor de dados: 52
Autores: Boas,Eduardo V. de B. Vilas; Chitarra,Adimilson B.; Menezes,Josivan B.
O objetivo deste trabalho foi o de estudar as mudanças estruturais da parede celular durante o armazenamento do melão 'Orange Flesh' submetido ao tratamento pós-colheita com CaCl2. Os teores de celulose, hemicelulose, substâncias pécticas e seu grau de esterificação, proteínas e cálcio foram determinados na parede celular da polpa dos frutos armazenados sob temperatura ambiente e refrigeração. O tratamento hidrotérmico por imersão em CaCl2 de melões 'Orange Flesh' armazenados sob temperatura ambiente determinou, aparentemente, um incremento na concentração de cálcio ligado à parede celular da polpa dos frutos, além de inibir parcialmente a degradação de substâncias pécticas e seu grau de esterificação e promover a solubilização de proteínas da parede...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melon cell wall; Melon postharvest; Calcium postharvest treatment.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400011

Biochemical taxonomy of Crenicichla (Pisces: Perciformes: Cichlidae) of the Iguaçu River, Brazil
Provedor de dados: 52
Autores: Renesto,Erasmo; Zawadzki,Cláudio Henrique; Revaldaves,Eloísa.
Samples of Crenicichla iguassuensis and a morph from the Iguaçu River which differed from C. iguassuensis by its lips morphology were examined by isozyme starch electrophoresis technique. The analysis of 27 enzyme loci exhibited a genetic identity of 0.993 between the two morphs. No diagnostic locus was found that permitted to distinguish the two Crenicichla morphs. The data obtained in this investigation did not support the existence of two distinct species for this genus in the Iguaçu River, which suggested that the morphs stood for two polymorphic forms of Crenicichla iguassuensis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isozyme polymorphism; Biochemical systematics; Biochemical taxonomy; Genetics of Crenicichla; Genetic identity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100003

Evaluation of the acerola juice concentrated by reverse osmosis
Provedor de dados: 52
Autores: Gomes,Eliane Rodrigues dos Santos; Mendes,Elisabete Scolin; Pereira,Nehemias Curvelo; Barros,Sueli Teresa Davantel de.
The aim of this study was to obtain the acerola juice using separation processes with membranes. The acerola pulp was initially defrosted and treated with 100 ppm of Citrozym Ultra L enzyme at 45ºC for one hour, then ultrafiltrated at 3 bar at 45ºC using 0.1 µm ceramic membrane, and concentrated by reverse osmosis using a spiral membrane of a compound film. The pressures on the reverse osmosis were 20, 30, and 40 bar at environmental temperature, thus, resulting a juice with 9.76, 14.56, and 17.36 ºBrix, respectively. The physicochemical characteristics of the juice were preserved and, studies on evaluation of the public acceptability, showed that 75% of the consumers liked the juice.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acerola; Ultrafiltration; Reverse osmosis; C Vitamin; Concentration.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400022

Infliximab treatment prevents hyperglycemia and the intensification of hepatic gluconeogenesis in an animal model of high fat diet-induced liver glucose overproduction
Provedor de dados: 52
Autores: Haida,Karissa Satomi; Bertachini,Gabriela; Tavoni,Thauany; Guilhermetti,Márcio; Loures,Marco Rocha; Bazotte,Roberto Barbosa.
The effect of infliximab on gluconeogenesis in an animal model of diet-induced liver glucose overproduction was investigated. The mice were treated with standard diet (SD group) or high fat diet (HFD group). HFD group were randomly divided and treated either with saline (100 µl/dose, ip, twice a day) or infliximab (10 µg in 100 µl saline per dose, ip, twice a day, i.e., 0.5 mg/kg per day). SD group also received saline. The treatment with infliximab or saline started on the first day of the introduction of the HFD and was maintained during two weeks. After this period, the mice were fasted (15 h) and anesthetized. After laparotomy, blood was collected for glucose determination followed by liver perfusion in which L-alanine (5 mM) was used as gluconeogenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infliximab; Hyperglycemia; TNF-alpha; Liver gluconeogenesis; Type 2 diabetes; Mouse.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300009

Purification and characterization of extracellular α-amylase from a thermophilic Anoxybacillus thermarum A4 strain
Provedor de dados: 52
Autores: Baltas,Nimet; Dincer,Barbaros; Ekinci,Arife Pinar; Kolayli,Sevgi; Adiguzel,Ahmet.
ABSTRACT α-Amylase from Anoxybacillus thermarum A4 was purified using ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography, with 29.8-fold purification and 74.6% yield. A4 amylase showed best performance for soluble potato starch hydrolysis at 70 °C and pH 5.5-10.5. A4 amylase was extremely stable at +4 °C, and the enzyme retained over 65% of its original α-amylase activity at 70 °C and 43% at 90 °C. The enzyme's Km values for soluble starch, amylopectin and amylose substrates were obtained as 0.9, 1.3 and 0.5 mg/mL, respectively. EDTA, Hg2+, B4O7 2-, OH-, CN- , and urea exhibited different inhibition effects; their IC50 values were identified as 8.0, 5.75, 16.5, 15.2, 8.2 and 10.9 mM, respectively. A4 amylase exhibited extreme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anoxybacillus thermarum A4; Extracellular α-Amylase; Highly thermostable; Purification.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100605

Fabrication and Characterization of Gliclazide Loaded Microcapsules
Provedor de dados: 52
Autores: Asad,Muhammad; Bashir,Sajid; Mahmood,Tariq; Nazir,Imran; Imran,Muhammad; Karim,Sabiha; ul Hassnain,Fakhar.
This study aimed to formulate, characterize and evaluate the Gliclazide (GLZ) microcapsules prepared with sodium alginate, guar gum and pectin in different ratios by ionotropic-gelation method. The microcapsules were evaluated against different parameters such as particle size and shape, Carr's index, Hausner's ratio, rheological studies and drug release kinetics. Fourier Transform Infra Red (FTIR) and Differential Scanning Calorimetric (DSC) studies demonstrated the absence of any drug - polymers interaction. Promising characteristics were observed in rheological behavior and release kinetics. The size of microcapsules and percentage yield was in the range of 676 to 727 µm and 69 to 77%, respectively. Scanning electron micrographs revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcapsules; Gliclazide; Ionotropic-gelation method; Guar gum; Pectin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600874

Oxygen consumption during mineralization of organic compounds in water samples from a small sub-tropical reservoir (Brazil)
Provedor de dados: 52
Autores: Cunha-Santino,Marcela Bianchessi da; Bianchini Júnior,Irineu.
Assays were carried out to evaluate the oxygen consumption resulting from mineralization of different organic compounds: glucose, sucrose, starch, tannic acid, lysine and glycine. The compounds were added to 1 l of water sample from Monjolinho Reservoir. Dissolved oxygen and dissolved organic carbon were monitored during 20 days and the results were fitted to first order kinetics model. During the 20 days of experiments, the oxygen consumption varied from 4.5 mg.l-1 (tannic acid) to 71.5 mg.l-1 (glucose). The highest deoxygenation rate (kD) was observed for mineralization of tannic acid (0.321 day-1) followed by glycine, starch, lysine, sucrose and glucose (0.1004, 0.0504, 0.0486, 0.0251 and 0.0158 day-1, respectively). From theoretical calculations and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxygen consumption aerobic mineralization Reservoir of Monjolinho (UFSCar - São Carlos; SP).
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400027

Evaluation of Dietary Supplement Intake (Yeast Tablets) on Pregnant Albino Rats and Their Fetuses (Implications of Yeast Tablets on Rats &amp;amp; Fetuses)
Provedor de dados: 52
Autores: Omar,Amel Ramadan; El-Din,Eman Youssri Salah; Abdelrahman,Heba Ali; Mohamed,Hanan Ramadan.
ABSTRACT Histopathological studies in placenta, liver, kidney, apoptotic DNA damage and p53 mutation estimation of pregnant rats and their offspring exposed to dietary supplement yeast tablets, were carried out. Pregnant female albino rats were orally administrated yeast tablets at concentration 41.1 mg/kg during gestation period. Hematoxylin and eosin stained sections were used for examination. Neutral comet assay was performed to assess the apoptotic DNA damage and single strand conformational analysis was performed to screen the mutations inductions in p53 exons 7 and 8. Statistical analysis is applied to found the relation of apoptotic fraction in liver, kidney and placenta of pregnant rats and their offspring. Oral administration of yeast tablets to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alteration; Comet assay; Single strand conformational analysis; Yeast tablets.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100420

Relation between citric acid production by solid-state fermentation from cassava bagasse and respiration of Aspergillus niger LPB 21 in semi-pilot scale
Provedor de dados: 52
Autores: Prado,Flávera Camargo; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
The aim of this work was to study the relation between citric acid production and respiration of Aspergillus niger LPB 21 in solid-state fermentation of cassava bagasse. The experiments were carried out in horizontal drum bioreactor coupled with a gas chromatography system. Fermentation was conduced for 144 h with initial substrate moisture of 60% using heat-treated cassava bagasse as sole carbon source. The exhausted air from the bioreactor was analyzed for the monitoring of CO2 produced and O2 consumed in order to estimate the biomass biosynthesis by the fungal culture. The metabolic activity of A. niger growth was associated to citric acid production. The system using FERSOL software determined 4.372 g of biomass/g of consumed O2. Estimated and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citric acid; Solid-state fermentation; Cassava bagasse; Horizontal drum bioreactor; Fungus respiration.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400004

Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds
Provedor de dados: 52
Autores: Katoch,Meenu; Salgotra,Aseem; Singh,Gurpreet.
This study aimed to screen the endophytic fungal species of ethano-medicinal plant Bacopa monnieri (L.) Pennell for their ability to produce antimicrobial substances against Bacillus subtilis, Pseudomonas aeroginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Candida albicans. Endophytes were also screened for their ability to produce amylase, cellulase, protease and lipase to evaluate their ecological role within the host plant. Twenty-six endophytes were isolated and seventeen were identified. All the isolated endophytes exhibited amylolytic activity. Lipolytic, cellulolytic, proteolytic activity was shown by 98, 28 and 31% isolates, respectively. Similarly, all the endophytes (100%) exhibited significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacopa monnieri; Antimicrobial; ITS 5.8S rDNA; Lipase; Amylase; Endophyte.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500714

First report of Epibiont ciliates (Ciliophora: Peritrichia) living on larvae of Leptophlebiidae (Ephemeroptera) from Brazil
Provedor de dados: 52
Autores: Abrahão,Daniel Peroni; Mello,José Leonardo; Gorni,Guilherme; Corbi,Juliano José.
ABSTRACT Epibiosis was understood as a commensalism relation between two or more organisms. However, some studies have shown that epibionts can cause deleterious effects to their host. Here, we reported the first report of epibiosis between protozoa Epibiont ciliates (Ciliophora: Peritrichia) living on larvae of Ephemeroptera from Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epibiosis; Protozoa; Ephemeroptera.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100401

Management of Root-Knot Disease on Tomato with Bioformulated Paecilomyces lilacinus and Leaf Extract of Lantana camara
Provedor de dados: 52
Autores: Udo,Idorenyin Asukwo; Osai,Ephraim Onoku; Ukeh,Donald Agioliwhu.
Glasshouse experiments were conducted to evaluate the efficacy of application frequency of a bioformulated Paecilomyces lilacinus in combination with five concentration of Lantana camara crude aqueous leaf extract against Meloidogyne incognita race I on tomato. The experiment was a 3x5 factorial laid out in a completely randomized design (CRD) with four replications. Each seedling was inoculated with 5000 eggs of M. incognita. Application of the bionematicide and L. camara leaf extract alone significantly (P≤ 0.05) inhibited root galling and egg production compared with their respective control. However, the severity of root galling and egg mass production was more significantly (P&lt; 0.05) suppressed with the application of P. lilacinus than L. camara...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biocontrol; Meloidogyneincógnita; Paecilomyceslilacinus; Lantanacâmara; Tomato; Botanical.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400486

Enhanced solubilization of iron and calcium phosphates by Aspergillus niger by the addition of alcohols
Provedor de dados: 52
Autores: Barroso,Cinthya Babá; Nahas,Ely.
The present study deals with the effect of increased concentrations of alcohols (methanol and ethanol) on the solubilization of FePO4 and CaHPO4 by Aspergillus niger. The efficiency of solubilization (ES) of inorganic phosphates, fungal growth, acid production and pH variation were determined. The concentrations of alcohols that most favored the solubilization of phosphates were 4% methanol and 3% ethanol. The ES of phosphates by the fungus in media containing methanol ranged from 11-96% and 0.4-87% for ethanol. The stimulation or inhibition of solubilization was dependent on greater or lesser fungus growth, acid production and decreased pH. These responses were supported by the correlation between the amount of phosphate dissolved and fungal growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Methanol; Growth; Acid production; Phosphate solubilization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200003

The male reproductive system and the effect of an extract of a medicinal plant (Hypericum perforatum) on the labeling process of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Hypericum perforatum (hiperico) is a plant that has been used to treat diseases and also inhibits rat and human vas deferens contractility. In nuclear medicine, stannous chloride (SnCl2) is used as a reducing agent to obtain radiopharmaceuticals labeling with technetium-99m. As the SnCl2 seems to have adverse effects related with the reproductive performance of male rabbits as well as the human consumption of hiperico might affect sexual function. In the present work, consistent results show significant changes on the blood constituents labeled by technetium-99m obtained from young rats under the effect of an hiperico extract as opposed to blood samples equally treated taken from elderly rat.. Supposedly, this extract could protect the male reproductive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stannous chloride; Young animals; Elderly animals; Sexual function; Technetium-99m; Hypericum perforatum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600011

Composition of omega-3 and omega-6 fatty acids in freeze-dried chicken embryo eggs with different days of development
Provedor de dados: 52
Autores: Campos,Célia Maria Teixeira de; Hamad,Antônio José Simões; Block,Jane Mara; Padilha,José Carlos Fiad; Ogliari,Paulo José; Barreira-Arellano,Daniel; Moreira,Rosana Nogueira Cavalcanti.
Fatty acids omega--3 and omega--6 composition and specially DHA were determined in freeze-dried chicken embryo eggs with pre-determined incubation periods. Fertile and embryo eggs presented palmitic (23.18 + 0.54%), stearic (7.70 + 0.28%), palmitoleic (3.00 + 0.19%), oleic (36.28 + 0.58%), linoleic (22.18 + 0.34%), linolenic (1.08 + 0.04%), arachidonic (2.04 + 0.03%), docosahexaenoic (0.91 + 0.03%), total omega-3 acids (2.26 + 0.10%) and total omega-6 acids (24.62 + 0.33%). There were no significant differences in total contents of omega-3 fatty acids (p=0.1226) between freeze-dried chicken embryo eggs with different incubation periods (3, 5, 7, 9, and 11 days) and fertile freeze-dried chicken eggs (day 0). However, there were significant differences in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken embryo eggs; Fatty acids omega-3 and omega-6; DHA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200009

Effects of natural banks of free-floating plants on zooplankton community in a shallow subtropical lake in Southern Brazil
Provedor de dados: 52
Autores: Gazulha,Vanessa; Montú,Mônica; Marques,David da Motta; Bonecker,Claúdia Costa.
The aim of this study was to test the effects of natural free-floating plants on zooplankton distribution in a shallow subtropical lake. First, the hypothesis that free-floating plants have an effect on physico-chemicals, leading to a decrease on nutrient availability and influencing the phytoplankton biomass and zooplankton community was tested. Second, the hypothesis that free-floating plants act as a refuge for zooplankton was tested. Three microhabitats were selected: free-floating plants, littoral area and open water. Results demonstrated that the effects of different microhabitats on phytoplankton biomass and physico-chemicals were not significant, indicating a weak influence of the plants. Zooplankton densities were higher in free-floating plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrophytes; Refuge; Spatial distribution; Tropical zooplankton.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400014

Genetic Variability of Brycon hilarii in a Repopulation Program
Provedor de dados: 52
Autores: Bignardi,Annaiza Braga; Povh,Jayme Aparecido; Alves,Mariana Srucki; Goes,Elenice Souza dos Reis; Filho,Ruy Alberto Caetano Corrêa; Castro,Reinaldo José; Barrero,Nelson Mauricio Lopera.
ABSTRACT Brycon hilarii, popularly called piraputanga in Brazil, is a species distributed throughout the whole basin of the river Paraguay. In recent years, the species has been on a repopulation program due to its remarkable decline as a wild species in the region. Assessment of the genetic diversity of broodstock and fingerling stocks in repopulation programs is basic to avoid genetic impacts on wild populations. The genetic variability of the wild population and of the broodstock and fingerling stocks of B. hilarii in a repopulation program in the river Itiquira MT Brazil will be determined. Seven microsatellite loci produced 52 polymorphic alleles and heterozygosity revealed rates between 0.5794 and 0.7204. FIS did not register any endogamy in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Microsatellites; Piraputanga.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100424

Heavy metal accumulation in the leaves, stem and root of the invasive submerged macrophyte Myriophyllum spicatum L. (Haloragaceae): an example of Kadin Creek (Mugla, Turkey)
Provedor de dados: 52
Autores: Yabanli,Murat; Yozukmaz,Aykut; Sel,Fatma.
In this study, the existence of heavy metals such as chromium (Cr), arsenic (As), cadmium (Cd), mercury (Hg) and lead (Pb) their distribution in the organs of plant and their bioaccumulation rates in water samples and Myriophyllum spicatum which were collected from the Kadin Creek in seasonal periods between 2011 and 2012 were analyzed. Heavy metal concentrations in the mineralized plant and water samples were determined with Inductive coupled plasma with mass spectroscopy (ICP-MS). Results showed the maximum heavy metal concentrations, in the root and minimum heavy metal concentrations, except chromium, in the stem. The distribution of heavy metals As, Cd, Hg and Pb was in the form of root &gt; leaf &gt; stem; the distribution of Cr was in the root &gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metals; Myriophyllum spicatum; Kadin Creek.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300018

The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS
Provedor de dados: 52
Autores: Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromelain; Aqueous two-phase systems; Partitioning; Characterization; Pineapple.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100017

Three new varieties in Ocimum L. (Lamiaceae)
Provedor de dados: 52
Autores: Albuquerque,Ulysses P. de.
Three new infraspecifc taxa in Ocimum are described from Northeastern Brazil, which were based in vegetative morphology, habit, stem indumentum or purple pigmentation; these were O. minimum var. religiosum Albuquerque var. nov., O. campechianum var. pubescens Albuquerque var. nov. and O. campechianum var. congestifolium Albuquerque var. nov.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocimum; Lamiaceae; New varieties.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100001

Allelopathic evidence in Brachiaria decumbens and its potential to invade the Brazilian Cerrados
Provedor de dados: 52
Autores: Barbosa,Elizabeth Gorgone; Pivello,Vânia Regina; Meirelles,Sérgio Tadeu.
The aim of this study was to look for evidence of allelochemicals in B. decumbens, in parts of the plant from where they could easily be released to the environment. The germination inhibition of Phalaris canariensis, Lactuca sativa (standard species) and Melinis minutiflora, another invasive African grass, was tested using B. decumbens germinating seeds and aqueous leachates of the roots, green and senescent leaves, at 5, 10 and 20% w/v. Both the germinating seeds and the aqueous leachates of B. decumbens reduced the germination of the species tested; the effectiveness of the aqueous leachates increased according to concentration. Apparently, the competitive advantage of B. decumbens in the cerrados could be amplified via allelopathy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allelopathy; Cerrado; Biological invasion; Brachiaria decumbens; Melinis minutiflora.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400021

Isolation in HRT-18 cells and molecular analysis of a BCoV strain from diarrheic feces of naturally infected calves
Provedor de dados: 52
Autores: Stipp,Danilo Tancler; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Bodnar,Lívia; Alfieri,Amauri Alcindo.
Bovine coronavirus (BCoV) may cause acute diarrhea in newborn calves, leading to significant economic losses for cattle farmers. There are several diagnostic techniques used to detect BCoV in calf fecal samples, but virus isolation still has advantages for antigenic and genomic characterization. This study describes the isolation in HRT-18 cells and molecular characterization of Brazilian BCoV wild-type strains. Three fecal samples from diarrheic 30 day-old calves were inoculated in HRT-18 cell monolayers, which were then evaluated for HA titers and tested using semi-nested PCR followed by RFLP and sequencing. Two samples were successfully isolated and presented HA titers of 16 and 32 units per 25 mL. The results were confirmed using semi-nested PCR and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Diarrhea; Bovine coronavirus; Cell culture; Molecular analyses.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700007

Identification of a fat-soluble fraction of Sparganium stoloniferum that inhibits cervical cancer HeLa cells
Provedor de dados: 52
Autores: Zhang,Junwu; Wei,Yahui.
ABSTRACT  Context The rizoma of Sparganium stoloniferum has been used as a traditional Chinese medicinal herb for thousands of years. Sparganium stoloniferum is a stasis-breaking drug to treat a wide range of diseases including cancer,however, its activity of extract on cervical cancer HeLa cells and the mechanisms remains unknown.  Objective This study aimed to screen Sparganium stoloniferum extract for its inhibitory effects on cervical cancer HeLa cells.  Materials and methods Sparganium stoloniferum was extracted with 95% ethanol under reflux, and the extracts were preliminary separated by silica gel column chromatography. MTT assay and flow cytometry were used to determine the inhibitory effects of three fractions on HeLa cells. In addition, GC-MS was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sparganium stoloniferum; Fat-soluble active fraction; Cytotoxicity; HeLa cells.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100337

Genetic control on the performance of common bean differential cultivars to Colletotrichum lindemuthianum races
Provedor de dados: 52
Autores: Gonçalves-Vidigal,Maria Celeste; Bonett,Lucimar Pereira; Vidigal Filho,Pedro Soares; Gonela,Adriana; Ribeiro,Aliny Simony.
The aim of this work was to perform genetic control on differential cultivars Michelite, Michigan Dark Red Kidney, Perry Marrow, Cornell 49-242, PI 207262, AB 136 and G 2333 and its diallel hybrids by using Hayman's methodology. The entire F1s populations were evaluated in relation to race 89, whereas the race 31, the diallel scheme did not include the cultivar G 2333. The results showed that the additive effects were predominant for the 31 race, while non-additive effects were more important for race 89. The results demonstrated a high coefficient of genetic determination for the race 31. The G 2333, AB 136 and PI 207262 cultivars should be recommended obtaining cultivar resistant to anthracnose by backcross programs because theyshowed the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diallel analysis; Genetic inferences.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400003

Commercial Biological Control Agents Targeted Against Plant-Parasitic Root-knot Nematodes
Provedor de dados: 52
Autores: Tranier,Marie-Stéphane; Pognant-Gros,Johan; Quiroz,Reynaldo De la Cruz; González,Cristóbal Noé Aguilar; Mateille,Thierry; Roussos,Sevastianos.
Root-knot nematodes are microscopic round worms, which cause severe agricultural losses. Their attacks affect the productivity by reducing the amount and the caliber of the fruits. Chemical control is widely used, but biological control appears to be a better solution, mainly using microorganisms to reduce the quantity of pests infecting crops. Biological control is developing gradually, and with time, more products are being marketed worldwide. They can be formulated with bacteria, viruses or with filamentous fungi, which can destroy and feed on phytoparasitic nematodes. To be used by the farmers, biopesticides must be legalized by the states, which has led to the establishment of a legal framework for their use, devised by various governmental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopesticides; Meloidogynespp.; Filamentous Fungi; Homologation; Production; Formulation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600831

Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer
Provedor de dados: 52
Autores: Oda,Sandra Helena Inoue; Nepomuceno,Alexandre Lima; Ledur,Mônica Corrêa; Oliveira,Maria Cristina Neves de; Marin,Silvana Regina Rockenbach; Ida,Elza Iouko; Shimokomaki,Massami.
Total RNA isolated from Pectoralis major muscle from PSE (L*24h&gt;53.0, pH&lt;5.8) and non-PSE (44&lt;"L*24h&gt;"53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the α-ryr and β-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p&lt;0.05) for β-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p&gt;0.05) in α-ryr gene expression regardless of line studied. The β-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of α-RyR/β-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant hyperthermia; Poultry; Gene expression; Real time PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600024

Behavior of Ilex paraguariensis St. Hilaire, 1822 to Meloidogyne incognita and M. paranaensis and their influence on development of plantlets
Provedor de dados: 52
Autores: Santiago,Débora Cristina; Krzyzanowski,Alaíde Aparecida; Homechin,Martin.
The influence of Meloidogyne incognita and M. paranaensis on the development of mate (Ilex paraguariensis ST. Hill) plantlets was assessed in greenhouse and laboratory. The experimental design was randomized. Every plot of each one of the 10 repetitions contained one mate plantlet , inoculated with 5,000 eggs of M. incognita (races 1, 2, 3 and 4) and M. paranaensis. After 120 days it was observed that mate was a reliable hostess to such nematodes and that they could finish their life cycle. Plant development was significantly modified by M. incognita, races 1 and 2. Dry and green matter weight of shoots changed according to treatment. No changes were observed in fresh root weight. The number of eggs per root was similar for M. incognita, races 1 and 2, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tea; Nematode; Root-knot.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200001

Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli
Provedor de dados: 52
Autores: Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de.
Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020

Analysis of the Impacts of the Electrification of the Vehicle Fleet in the Electric Power System in Curitiba
Provedor de dados: 52
Autores: Brati,Rossetto, Matheus; Maria,Lourenço, Elizete.
ABSTRACT The Market share of electrical vehicles has been rising in the past few years and tends to rise even more. According to the International Energy Agency, an increase of 50% in the number of electrical vehicles is expected. Whilst that increase is beneficial from a greenhouse gases emissions drop point of view, that rise could, on the other hand, represent a major increase in the electrical power consumption. Besides, other problems such as harmonics and overloads could emerge from the massive connection of electrical vehicles to the electrical grid. Therefore, it is necessary to perform studies and simulations in order to estimate those problems and to mitigate the problems related to the inevitable expansion of the electric vehicle fleet in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electric Vehicles; Distributed Generation; Vehicle-to-Grid; Ancillary Services; Smart Cities; MatPower.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200202

Methotrexate inhibits integrin adhesion molecules in the mouse model of pleurisy induced by carrageenan
Provedor de dados: 52
Autores: Dalmarco,Eduardo Monguilhott; Fröde,Tânia Silvia.
The aim of this work was to analyze the effect of methotrexate (MTX) upon leukocyte migration and expression of adhesion molecules CD11a/CD18 in the lung, 4 and 48 h after inflammation induction by carrageenan in mice. The results showed that MTX significantly decreased leukocyte influx and CD11a expression in the lung at 4 and 48 h of pleurisy (P &lt; 0.01). MTX also inhibited CD18 expression at 4 h but not 48 h of pleurisy (P &lt; 0.01). These results proved that MTX at the studied doses had important anti-inflammatory properties, acting primarily on leukocyte migration from the pleural cavity to the lung via inhibition of CD11a/CD18 expression in the mouse model of inflammation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methotrexate; CD11a/CD18 adhesion molecules; Leukocyte migration; Pleurisy; Mice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500005

Anatomy of the developing fruit of Metrodorea nigra A. St.-Hil. (Rutaceae)
Provedor de dados: 52
Autores: Souza,Luiz Antonio de; Rosa,Sônia Maciel da; Moscheta,Ismar Sebastião.
The pericarp and seed in development of Metrodorea nigra were investigated structurally.The flowers and fruits in different stages of maturation were fixed in FAA 50 and cross-and longitudinally sectioned, according to usual techniques. The differentiated epicarp was represented by the periderm of subepidermal origin. The mesocarp was parenchymatous. The endocarp was sclerenchymatous and originated from the meristematic action of the inner epidermis of the ovary. The seed was endotestal and originated from an anatropous, bitegmic and crassinucellate ovule. The nucellus and the endosperm occurred in small quantity in the mature seed. The embryo was straight with thick cotyledons. The seed and the endocarp were ejected in the autochory process. The fruit was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Metrodorea nigra; Rutaceae; Fruit; Seed.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600012

Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products
Provedor de dados: 52
Autores: Santos,Édilla Ribeiro dos; Teles,Zozilene Nascimento Santos; Campos,Núria Mariana; Souza,Diogo Angeli Jacinto de; Bispo,Aline Simões da Rocha; Nascimento,Rodrigo Pires do.
Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylases; Sugarcane bagasse; Sisal waste; Streptomyces sp.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020

Analysis of biological parameters of Boophilus microplus Canestrini, 1887 exposed to entomopathogenic nematodes Steinernema carpocapsae Santa Rosa and All strains (Steinernema: Rhabditida)
Provedor de dados: 52
Autores: Freitas-Ribeiro,Gláucia Marques; Furlong,John; Vasconcelos,Viviane Oliveira; Dolinski,Cláudia; Loures-Ribeiro,Alan.
Engorged and partially engorged females of Boophilus microplus were exposed to 600; 3,000; 6,000 and 30,000 infective juveniles of Steinernema carpocapsae Weiser, 1955 Santa Rosa and All strains per dish, under lab conditions. Eggs weight, pre-laying period, laying period, survival period, reproductive efficiency rate, percentage of larval eclosion and lethal concentrations of 50% and 90% (CL50 and CL90) were calculated for engorged females. In the case of partially engorged females, only egg weight, survival period and CL50 and CL90 were calculated. All biological parameters of engorged or partially engorged females were altered by exposition of S. carpocapsae infective juveniles (IJs), Santa Rosa and All strains (p&lt;0.05). The increase in the response...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boophilus microplus; Steinernema carpocapsae; Entomopathogenic nematodes; Cattle tick; Reproduction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800007

Microbial rennet produced by Mucor miehei in solid-state and submerged fermentation
Provedor de dados: 52
Autores: Silveira,Guilherme Garcia da; Oliveira,Gustavo Monteiro de; Ribeiro,Eloizio Julio; Monti,Rubens; Contiero,Jonas.
The aim of this work was to study the effect of carbon and nitrogen sources in submerged fermentation, and the casein effect on solid-state fermentation on rennin production by Mucor miehei. Biomass peaks reached 6.7; 8.1 and 8 g/L and enzymatic production peaks of 1,066; 857 and 480 Soxhlet Units (S.U.) for glucose concentration of 18; 25 and 35 g/L respectively. Flasks with baffles showed biomass peaks of 6.7; 8.3 and 10 g/L and enzyme activity peaks of 648; 279 and 300 S.U. for the same glucose concentration. The values of 923 and 667 S.U. were obtained when corn steep liquor and Proflo (Cottonseed Nutrients from Traders ®) were used. In SSF system the enzymatic activities were 414, 264 and 167 S.U., when using HCl 0.2 N, 0.3 N and 0.4 N respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucor miehei; Rennin; Clotting activity; Casein; Solid-state fermentation; Submerged fermentation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800009

Influence of agitation speeds and aeration rates on the Xylanase activity of Aspergillus niger SS7
Provedor de dados: 52
Autores: Bakri,Yasser; Mekaeel,Alaa; Koreih,Amal.
In this study, the effect of agitation and aeration rates on xylanase activity of Aspergillus niger SS7 in 3-litre stirred tank bioreactor was investigated. The agitation rates tested were 100, 200 and 300 rpm at each airflow rates of 0.5, 1.0 and 1.5 vvm. The maximum xylanase activity in mono- agitator system was at the agitation speed of 200 rpm and aeration rate of 1.0 vvm. In bi-agitator system, at low agitation speed (100 rpm), the xylanase activity was enhanced by 13% compared to mono- agitator system for an aeration rate of 1.0 vvm. Xylanase productivity in continuous culture was higher by approximately 3.5 times than in batch culture.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Agitation; Aeration; Xylanase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400003

Bioremediation of herbicide velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms)
Provedor de dados: 52
Autores: Ramos,Márcio Antônio Gomes; Yoshioka,Sergio Akinobu.
This work assessed the bioremediation of herbicide Velpar K®, in vitro in aqueous solution, used against weeds in sugar cane in São Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K®in the gel. The analysis of high performance liquid chromatography (HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Velpar K®; Herbicide; Bioremediation; Effective microorganisms.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100018

Evaluation of Anticancer Activity of Extracted Flavonoids from Morus Alba Leaves and its interaction with DNA
Provedor de dados: 52
Autores: Fallah,Soudabeh; Hajihassan,Zahra; Zarkar,Nasrin; Chadegani,Azra Rabbani-; Mohammadnejad,Javad; Hajimirzamohammad,Milad.
ABSTRACT Morous Alba, known as white mulberry contains many oxidative flavonoids, widely used in the treatment of many diseases like hyperglycemia, inflammation, fever and cancer. In the present study we investigated the interaction of extracted flavonoids from Iranian Morus Alba leaves with DNA as a main target for anticancer drugs. Various spectroscopic techniques (UV/Vis, CD and Fluorescence Spectroscopy) were used to detect the interaction. In vivo studies also were done to confirm the effectiveness of the extracted flavonoids. The spectroscopic results showed that the extracted flavonoids bind to DNA especially to the sugar-phosphate backbone and making DNA conformational changes upon this binding. Experiments on the cancerous mice with solid tumors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavonoids; Anticancer activity; DNA interaction; Morus Alba.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100002

Photosynthesis, Growth and Development of Tabebuia avellanedae Lor. ex Griseb. (Bignoniaceae) in Flooded Soil
Provedor de dados: 52
Autores: Davanso,Viviane M.; Souza,Luiz A. de; Medri,Moacyr E.; Pimenta,José A.; Bianchini,Edmilson.
Morphological, anatomical and ecophysiological modifications caused by flooding in the growth and development of Tabebuia avellanedae Lor. ex Griseb. (Bignoniaceae) were studied and correlated with tolerance to the excess of water in the soil. Three-month-old plants, grown in a greenhouse, underwent a period of 56 days of flooding and a post-flooding period. Photosynthesis rate and growth decreased in T. avellanedae when flooding duration increased. Though not much tolerant, plant adapted itself to short flooding periods. This relative tolerance was probably due to the capacity of T. avellanedae in developing structures which lessened flooding effects and promoted internal diffusion of oxygen from the aerial part to the roots, such as stem fissures,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inundation; Oxygen deprivation; Ecological anatomy; Tabebuia avellaneadae.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300016

Effect of PONNEEM# on Spodoptera litura (Fab.) and its compatibility with Trichogramma chilonis Ishii
Provedor de dados: 52
Autores: Packiam,Soosaimanickam Maria; Ignacimuthu,Savarimuthu.
Antifeedant and growth regulating activities of PONNEEM, an oil formulation containing neem and pungam (karanj) oils were evaluated along with individual neem and karanj oils and Nimbicidine, a commercial neem-based pesticide against fourth instar larvae of Spodoptera litura (Fab.). Among all the treatments, PONNEEM recorded the maximum antifeedant activity (88.6%) at 0.6%. Neem and karanj individual treatments as well as PONNEEM extended larval duration compared to control. Pupal weight and fecundity were significantly reduced and pupal period was greatly increased by PONNEEM treatment compared to other treatments. The PONNEEM was found to be compatible with Trichogramma chilonis Ishii, an egg parasitoid of many lepidopteran pests, at 0.15, 0.3 and 0.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oil formulation; Spodoptera litura; Antifeedant activity; Growth regulating activity; Trichogramma chilonis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200016

Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation
Provedor de dados: 52
Autores: Uberti,Marcela Fornari; Vieira,Felipe do Nascimento; Salência,Helena Ragibo; Vieira,Genyess da Silva; Vinatea,Luis Alejandro.
Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants such as dimethyl sulfoxide (DMSO) and ethylene glycol (EG) at 10% concentration. Each treatment was divided in subgroups for storage in liquid nitrogen during 0, 30, 60 and 90 days, in triplicate. After thawing at 25ºC for 40 seconds, cell viability in the suspensions was analyzed under the microscope in eosin-nigrosin stain and flow cytometry. There were no significant differences between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryogenics; Semen; Marine shrimp; Cell viability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300010

Hematological and cerebrospinal fluid changes in cattle naturally and experimentally infected with the bovine herpesvirus 5
Provedor de dados: 52
Autores: Lisbôa,Júlio Augusto Naylor; Isernhagen,Allan Jürgen; Borges,Alexandre Secorun; Amorim,Rogério Martins; Balarin,Mara Regina Stipp; Lunardi,Michele; Alfieri,Amauri Alcindo.
Bovine herpesvirus 5 (BoHV-5) meningoencephalitis is one of the main causes of mortality by encephalopathy in Brazilian cattle herds. However, the neurological signs observed are common to several encephalopathies and do not contribute decisively to a diagnosis. In order to verify hematological and cerebrospinal fluid (CSF) changes, blood and CSF samples from naturally and experimentally infected bovines were evaluated. In natural cases (n=17), the samples were collected only once, and in experimental cases (n=7), the samples were sequentially obtained throughout disease progression. While routine methods were used to examine the samples, BoHV-5 infection was confirmed by a PCR assay. Blood analyses did not reveal any consistent hematological alterations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Meningoencephalitis; Herpesviruses; Complete blood count; Cerebrospinal fluid; Diagnosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700010

The Influence of Ca2+ on Gluconeogenesis Stimulation by Glucagon in the Liver of Arthritic Rats
Provedor de dados: 52
Autores: Kelmer-Bracht,Ana M.; Fedatto-Júnior,Zélio; Ishii-Iwamoto,Emy L.; Caparroz-Assef,Silvana M.; Bracht,Adelar.
Ca2+ participates in the stimulation of hepatic gluconeogenesis by glucagon and there is evidence that Ca2+ fluxes are modified in arthritic rats. These findings raise the question whether hepatic gluconeogenesis in arthritic rats responds differently to glucagon and Ca2+. The experimental system was the isolated perfused rat liver. In the presence of Ca2+, stimulation of hepatic gluconeogenesis by glucagon in arthritic rats was equal to that in normal rats in absolute terms, but higher in relative terms (104.5 and 45.2%, respectively). In the absence of Ca2+, however, stimulation of hepatic gluconeogenesis by glucagon in arthritic rats was smaller in both absolute and relative terms (18.5 and 41.9%, respectively). It can be concluded that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthritis; Liver; Gluconeogenesis; Glucagon.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300009

Peroxidase from peach fruit: thermal Stability
Provedor de dados: 52
Autores: Neves,Valdir Augusto; Lourenço,E. J.
Peroxidase from peach fruit was purified 28.9-fold by DEAE-cellulose, Sephadex G-100 and hydroxylapatite chromatography. The purified enzyme showed only one peak of activity with an optimum pH of 5.0 and temperature of 40ºC. The calculated activation energy (Ea) for the reaction was 7.97 kcal/mol. The enzyme was heat-labile in the temperature range of 60 to 80ºC with a fast inactivation at 80ºC. PAGE of the inactivation course at 70ºC showed only one band of activity. Different sugars increased the heat stability of the activity in the following order: sucrose&gt;lactose&gt;glucose&gt;fructose. Measurement of residual activity showed a stabilizing effect of sucrose at various temperature/sugar concentrations (10 to 40%, w/w) with the Ea for inactivation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peach peroxidase; Purification; Heat stability; Regeneration.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200002

Lipase production by solid-state fermentation in fixed-bed bioreactors
Provedor de dados: 52
Autores: Cavalcanti,Elisa d'Avila Costa; Gutarra,Melissa Limoeiro Estrada; Freire,Denise Maria Guimarães; Castilho,Leda dos Reis; Sant'Anna Júnior,Geraldo Lippel.
In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid-state fermentation; Fixed-bed bioreactors; Lipase; Penicillium simplicissimum.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400010

Involvement of transforming growth factor beta-1 (TGFβ1) cytokine and FOXP3 transcription factor genetic polymorphisms in hematological malignancies
Provedor de dados: 52
Autores: Vitiello,Glauco Akelinghton Freire; Guembarovski,Roberta Losi; Oliveira,Carlos Eduardo Coral de; Amarante,Marla Karine; Perim,Aparecida de Lourdes; Watanabe,Maria Angelica Ehara.
Hematological malignancies (HM) are a group of neoplastic diseases that arise from hematologic cell lineages. Transforming growth factor beta 1 (TGFβ1) is shown to negatively regulate normal and malignant hematopoiesis and, in immunological context, to promote T cell exhaustion and generation of regulatory T cells, which are shown to be deleterious in cancer, by the induction of transcription factor FOXP3 expression. The present study aimed to evaluate TGFB1 exon-1 rs1800470 and FOXP3 intron-1 rs2232365 polymorphisms in relation to HM susceptibility. DNA was extracted from blood samples of 43 HM patients and 142 neoplasia-free individuals and polymorphisms were analyzed by allelic-specific PCR. Association analysis was assessed by the Odds Ratio (OR) with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematological malignancies; TGFB1; FOXP3; Genetic polymorphism; Susceptibility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400553

Dietary utilisation of protein and energy from fresh and ensiled coffee pulp by the Nile tilapia, Oreochromis niloticus
Provedor de dados: 52
Autores: Moreau,Yann; Arredondo,Jose-Luis; Perraud-Gaime,Isabelle; Roussos,Sevastianos.
Dietary protein and energy utilisation of diets containing fresh and ensiled coffee pulp were studied on 3.2 ± 0.2 g Nile tilapia for 28 days. Diets formulation and feeding were designed on the basis of daily dietary protein and energy allowance. A control diet A (100 % protein and 100 % energy allowance) corresponding to 15 g CP kg-1 day-1 and 750 kJ kg-1 day-1, a low protein control diet B (80 % protein and 100 % energy allowance), two diets C and E (100 % protein and 100 % energy allowance) where 20 % of protein were supplied by coffee pulp, and two diets D and F with the same amount of coffee pulp than in C and E and supplementation in non-protein energy. Inclusion of coffee pulp in the diet strongly impaired growth and feed utilisation. Silage process...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee pulp; Silage; Aquaculture; Cichlids; Feedstuff; Fish.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200014

Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals
Provedor de dados: 52
Autores: Watanabe,Maria Angelica Ehara; Miquelão,Ana Karina Melim Benthien; Oliveira,Carlos Eduardo Coral de; Oliveira,Karen Brajão de; Nasser,Thiago Franco; Aoki,Mateus Nóbrega; Suzuki,Patrícia Sayuri; Amarante,Marla Karine; Venâncio,Emerson José; Azevedo,Elbens Marcos Minoreli de.
The purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical cancer. GBV-C/HGV RNA was detected by reverse transcriptase and polymerase chain reaction (RT-PCR). Only one woman presented co-infection with human papilloma virus (HPV). The GBV-C/HGV RNA was detected in 13/73 (17.57%) healthy women and it's prevalence in participating women between 28-43 years old was 53.85%. No association was found with GBV-C/HGV for the age of first sexual intercourse and number of pregnancies. In GBV-C/HGV RNA positive women, 69.23% were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GBV-C/HGV RNA; Healthy women; Cervicovaginal smears.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500007

Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
Provedor de dados: 52
Autores: Oliveira,Arlem Nascimento de; Oliveira,Luiz Antonio de; Andrade,Jerusa Souza.
Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizobium; Bradyrhizobium; Enzymatic activities; Organic surfactants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024

Insilico Analysis of Phytoconstituents from Allium sativum as Potential Inhibitors of Inha in Mycobacterium tuberculosis
Provedor de dados: 52
Autores: Kannan,Rmaraj; Alharbi,Naiyf S.; Kadaikunnan,Shine; Rajaram,Shyam Kumar; Alexander,Ronaldo Anuf.
ABSTRACT Tuberculosis is leading cause of death among the global bacterial infections. The main causative for tuberculosis is Mycobacterium tuberculosis, which will survive in its host human being for decades in latent or chronic levels. In addition, the late multidrug resistance at a disturbing rate accompanies the appearance of tuberculosis. The quick spread of resistance to initial stage treatment medications has redirected the focus of the medical community in the creation of an array of new drug against Mycobacterium tuberculosis. The InhA protein is a component of Fatty acid synthetase (FAS) II and exhibits an NADH reliant enoyl-ACP reductase activity. InhA is a vital enzyme of M.tuberculosis in control of cell wall synthesis, which can turn out to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; InhA; Enoyl ACP reductase; Allium sativum; Moldock.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100329

Mineral composition and dry mass production of the corn plants in response to phosphorus sources and aluminum concentration
Provedor de dados: 52
Autores: Batista,Marcelo Augusto; Pintro,José Carlos; Costa,Antonio Carlos Saraiva da; Tormena,Cássio Antonio; Bonato,Carlos Moacir; Batista,Michelli Fernandes.
The corn plants were evaluated with different phosphate fertilizer sources and aluminum (Al) concentrations in a sandy substrate. A totally randomized experiment design was set up with two corn plants in the pots containing 2 kg of a sandy substrate, two phosphate sources (Triple Super Phosphate - TSP or Arad Phosphate - AP) and four Al concentrations. When Al concentrations increased, pH (CaCl2) substrate values decreased. There was an increase in the calcium and phosphorus contents in the sandy substrates that received the TSP and AP sources. The calcium, magnesium, phosphorus, and potassium concentrations of the corn plant's shoot were higher in the TSP than without P and AP sources. When the Al concentration increased, the concentration values of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Phosphate fertilizers; Solubility; Toxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300004

Feeding of two sympatric species of Characidium, C. lanei and C. pterostictum (Characidiinae) in a coastal stream of Atlantic Forest (Southern Brazil)
Provedor de dados: 52
Autores: Aranha,José Marcelo R.; Gomes,José Henrique C.; Fogaça,Fábio N. O.
The feeding strategy of Characidium lanei and C. pterostictum was studied in the "Cabral" stream, a coastal stream of the Atlantic Forest, in southern Brazil. The samples were taken using electric fishing between March/95 and February/96. We studied about 15 stomach contents for each species in each season of the year. The stomach contents of the studied species were composed of 39 different food items, 28 in C. lanei and 24 in C. pterostictum. Chironomidae larvae and Ephemeroptera nymph were the most important items for C. lanei, except in the summer when Acarina and not identified Arthropoda were the most important items. Chironomidae and Simulidae larvae were the most important items in C. pterostictum. These results indicated an insectivorous habits...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding; Sympatric species; Atlantic Forest; Coastal Stream; Characidiinae; Characidium.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500013

Batch and continuous extraction of bromelain enzyme by reversed micelles
Provedor de dados: 52
Autores: Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reversed micelles; Liquid-liquid extraction; Bromelain; Pineapple; Optimization; Surface response.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021

Erratum: Effects of Sodium Nitrate and Mixotrophic Culture on Biomass and Lipid Production in Hypersaline Microalgae Dunaliella Viridis Teod
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100904

Production of recombinant proteins in milk of transgenic and non-transgenic goats
Provedor de dados: 52
Autores: Moura,Raylene Ramos; Melo,Luciana Magalhães; Freitas,Vicente José de Figueirêdo.
Among all the transgenic mammalians produced so far, goats have represented an excellent model of transgenesis when considering the factors such as the market demand for protein, volume of milk produced per lactation and reproductive rate. Various recombinant proteins have been obtained from the transgenic and non-transgenic goats, and among these, human antithrombin, produced by the transgenic goats, was the first recombinant protein of animal origin to be released as a drug for the clinical use in humans. This review reports the aspects inherent to the production of recombinant proteins in the goats, from the production of the animal bioreactors up to the expression of these proteins in their milk.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenesis; Goat; Recombinant protein; Milk.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500010

Somatic embryogenesis of Neoglaziovia variegata (Arruda) Mez, an important source of fiber from native Brazilian bromeliads
Provedor de dados: 52
Autores: Silveira,Daniela Garcia; Lino,Lucimeire Souza Morais; Souza,Antônio da Silva; Souza,Fernanda Vidigal Duarte.
Neoglaziovia variegata (Arruda) Mez, a Bromeliad endemic to the Brazilian Caatinga and source of fiber for many different products, is considered an endangered species. Leaf and stem segments were cultivated on Murashige and Skoog (MS) medium with combinations of 2,4- dichlorophenoxyacetic acid (2,4-D) (0, 9, 18 and 27 µM) and glutamine (0, 50, 100, 150, 200 mg L-1) for the induction of embryogenic calli. Leaf segments did not present a favorable response for the combinations analyzed. The best response for embryogenic calli frequency with the best distribution of somatic embryos on the surface and better conversion rate was obtained using the stems explants in the medium containing 18 µM 2,4-D and 100 mg L-1 glutamine. The embryogenic calli obtained from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caroá Bromeliaceae tissue culture 2; 4-D glutamine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400004

Influence of passion fruit juice on colour stability and sensory acceptability of non-sugar Yacon-based pastes
Provedor de dados: 52
Autores: Granato,Daniel; Castro,Inar Alves de; Piekarski,Flávia Vilas Boas Wiecheteck; Benincá,Cristina; Masson,Maria Lucia.
This work was aimed at studying the influence of PFJ and/or sodium disulphite on Yacon pulp colour, and evaluating the sensory attributes of Yacon non-sugar pastes. A colour kinetic evaluation of browning in 11 treatments containing Yacon tubers, yellow passion fruit juice (PFJ) and/or sodium disulphite, over the course of 240 minutes was studied to inactivate the PPOs activity. The evaluation of the colour alterations of Yacon tuber by the addition of variable amounts of PFJ and sodium disulphite enabled identification of efficient treatments for the inhibition of browning: T7 (300 g kg -1 PFJ), T8 (400 g kg -1 PFJ), and T10 (150 g kg -1 PFJ + 0.25 g kg -1 sodium disulphite). These treatments presented high colour intensity (C*) and an insignificant (P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smallanthus sonchifolius; Diabetes; Passion fruit; Sensory analysis; Kinetics; Polyphenol oxidases.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100020

Submicroscopical Features of Leaves of Xyris Species
Provedor de dados: 52
Autores: Sajo,Maria das Graças; Machado,Silvia Rodrigues.
The leaf ultrastructure of five Xyris species were examined using scanning electron microscope (SEM), transmission electron microscope (TEM) and histochemical methods. All studied leaves show some features in epidermis and mesophyll, which were of considerable adaptative significance to drought stress. Such features included the occurrence of a pectic layer on the stomatal guard cells and the presence of a network of pectic compounds in the cuticle. Pectic compunds were also in abundance in lamellated walls of the mesophyll cells and on the inner surface of the sclerified cell walls of the vascular bundle sheaths. There were also specialized chlorenchymatous "peg cells" in the mesophyll and drops of phenolic compounds inside the epidermal cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xyris; Ultrastructure; Leaf.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400011

A comparison of methods to determine tannin acyl hydrolase activity
Provedor de dados: 52
Autores: Aguilar,Cristóbal; Christopher,Augur; Viniegra-González,Gustavo; Favela,Ernesto.
Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tannin acyl hydrolase; Assay; Polyurethane foam; Solid state fermentation; Tannase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300014

Breves Notícias Sobre a Geologia dos Estados do Paraná e Santa Catarina
Provedor de dados: 52
Autores: Maack,Reinhard.
The present short survey of the geology of the States of Paraná and Santa Catarina was written as a text to accompany sketches for the revision of the GEOLOGICAL MAP OF SOUTH AMERICA. It contains the results of geologic research up to the year 1947 in most concise form, without going into detail. As requested, the occurrence of useful minerals was not considered in the summary, and the list of bibliographical references has been condensed to the uttermost. Inasmuch as the author treated the Passa Dois Series in more detail, due to the reorganization of the Gondwana beds, the Archean and the Algonkian had to be reduced to the minimum. This was possible, since the innumerable details of the phenomena in the crystalline complex could not be represented in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geology; Gondwana beds; Archean; Algonkian; Crystalline complex; Passa Dois Series.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500010

Effects of sub-lethal exposure of lead acetate on histopathology of gills, liver, kidney and muscle and its accumulation in these organs of Clarias gariepinus
Provedor de dados: 52
Autores: Al-Balawi,Hmoud Fares Alkahem; Al-Akel,Ali Sulaiman; Al-Misned,Fahad; Suliman,El Amin Mohamed; Al-Ghanim,Khalid Abdullah; Mahboob,Shahid; Ahmad,Zubair.
The aim of this work was to study the effects of sub-lethal exposure of lead acetate on the histopathology of the gills, liver, kidney and muscle and its accumulation in these organs of Clarias gariepinus. Results showed that lead accumulation in the tissues of C. gariepinus was dependent on the exposure period and lead concentration. Gills and liver were the predominant storage tissue and the order of accumulation in tissues was gill &gt; liver &gt; kidney &gt; muscle. Some structural changes were observed in different organs, especially in the gills of the fishes exposed to lead acetate. Epithelial hypertrophy and epithelial lifting were apparent in the gills of exposed fishes. The degeneration of cytoplasm and secondary lamellae was also observed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clarias gariepinus; Lead acetate; Accumulation; Histopathology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200015

Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane
Provedor de dados: 52
Autores: Severo Júnior,João Baptista; Oliveira,Laura Sampaio de Sá; Sardeiro,Fernanda Silva; Souza,Roberto Rodrigues de; Lopes,Francisco Luiz Gumes; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
This work aimed to study the pH and the transmembrane pressure effects during the recovery of alpha and beta amylases enzymes from corn malt (Zea mays) by hollow fiber membrane. The optimal condition was obtained for a statistical model, established by response surface methodology (RSM). The response surface analysis showed that the best operation condition for amylolitics enzymes recovery by hollow fiber membrane was 0.05 bar and pH 5.00, while the enzymes were purified about of 26 times.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hollow fiber membrane; Corn malt; Alpha and beta amylase; RSM.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400017

Alpha amylase from a fungal culture grown on oil cakes and its properties
Provedor de dados: 52
Autores: Ramachandran,Sumitra; Patel,Anil K.; Nampoothiri,Kesavan Madhavan; Chandran,Sandhya; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok.
Solid-state fermentation was carried out for the production of alpha-amylase using Aspergillus oryzae. Different oil cakes such as coconut oil cake (COC) sesame oil cake (SOC), groundnut oil cake (GOC), palm kernel cake (PKC) and olive oil cake (OOC) were screened to be used as substrate for the enzyme production and also compared with wheat bran (WB). GOC was found to be the best producer of the enzyme among these. Combination of WB and GOC (1:1) resulted higher enzyme titres than the individual substrates. Maximum amount of enzyme (9196 U/gds) was obtained when SSF was carried out using WB + GOC, having initial moisture of 64% and supplemented with lactose and ammonium nitrate (1% each) at 30ºC for 72h using 2 mL spore suspension (6x10(7)spores/ml)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha amylase; Oil cakes; Solid-state fermentation; Partial purification; Aspergillus oryzae.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200019

Microbial production of citric acid
Provedor de dados: 52
Autores: Vandenberghe,Luciana P. S; Soccol,Carlos R; Pandey,Ashok; Lebeault,Jean-Michel.
Citric acid is the most important organic acid produced in tonnage and is extensively used in food and pharmaceutical industries. It is produced mainly by submerged fermentation using Aspergillus niger or Candida sp. from different sources of carbohydrates, such as molasses and starch based media. However, other fermentation techniques, e.g. solid state fermentation and surface fermentation, and alternative sources of carbon such as agro-industrial residues have been intensively studied showing great perspective to its production. This paper reviews recent developments on citric acid production by presenting a brief summary of the subject, describing micro-organisms, production techniques, and substrates, etc.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citric acid; Aspergillus niger; Submerged fermentation; Solid state fermentation; Substrates.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300001

ATP - bioluminescence as a technique to evaluate the microbiological quality of water in food industry
Provedor de dados: 52
Autores: Costa,Patrícia Dolabela; Andrade,Nélio José de; Passos,Frederico José Vieira; Brandão,Sebastião César Cardoso; Rodrigues,Carolina Gonçalves Freire.
ATP-bioluminescence was used to evaluate the microbiological quality of water samples collected from the water supply, the water treatment system and from a dairy plant, including ammonia-cooling water and industrial water. For industrial water, there was relation between the ATP-bioluminescence technique and microbial count. There were no differences (p&gt;0.05) between water supply and ammonia-cooling water samples for total and free ATP concentrations nor for the microbial counts. Different microbial ATP concentrations were found for these water samples. The results suggested that the physical chemical quality of ammonia cooling water decreased the RLU measurements slightly. It could be concluded that the total ATP concentration was the most effective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water quality; ATP-bioluminescence measurement; Food industry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300010

Gas exchange and leaf specific mass of different foliar cohorts of the wintergreen shrub Aristotelia chilensis (Mol.) stuntz (Eleocarpaceae) fifteen days before the flowering and the fall of the old cohort
Provedor de dados: 52
In the beginning of the spring Aristotelia chilensis sheds the old cohort, forms a new one and flowers. Fifteen days before the flowering and the fall we measured net photosynthesis (A), transpiration (E), water use efficiency (WUE), specific leaf mass (SLM) and area and mass of leaves per branch in old and new cohorts. Under low irradiance the old cohort exhibited higher mean values of A and WUE on area bases (P&lt;0.05). Under high irradiance A and WUE on mass bases were higher in new cohort due to its lower SLM (42.10 gm-2) in relation to old cohort (79.53 gm-2). Because old cohort showed 3.7 times more dry mass per branch it was able to counterbalance its lower performance on mass bases. It was concluded that old cohort was an important source of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aristotelia chilensis; Foliar cohorts; Photosynthesis; Specific leaf mass.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300009

Effects of plant cover on the macrofauna of Spartina marshes in northern Brazil
Provedor de dados: 52
Autores: Braga,Cesar França; Beasley,Colin Robert; Isaac,Victoria Judith.
Data on macrofauna density and diversity, and the height and density of Spartina brasiliensis, were obtained from salt marsh beds of a tropical estuary in northern Brazil. Sampling was carried out at four distinct times of the year, during the wet and dry seasons and in the transition periods between these. Sampling was also carried out in salt marshes of three size classes, small, medium and large. Variables were analyzed in relation to time of year and salt marsh size class. Overall, 46 taxa were found, with polychaetes, isopods and the gastropod Neritina virginea dominating the fauna. Macrofauna density and diversity were positively correlated with culm density, indicating a possible role in protection from predation. All the three variables were higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrofauna; Spartina; Density; Diversity; Seasonality; Tropical estuary.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600013

Effect of the dry matter intake level on the sanguine profile of glucose, insulin, urea, estrogen and progesterone and concentration of IGF-I in the follicular liquid of crossbred heifers
Provedor de dados: 52
Autores: Rigolon,Luiz Paulo; Prado,Ivanor Nunes do; Cavalieri,Fábio Luiz Bim; Nascimento,Willian Gonçalves do; Copovila,Luiz Carlos; Ramos,Fabíola dos Santos; Moreira,Fernanda Barros.
The aim of this work was to study effect of the dry matter intake level on the concentrations of glucose, insulin, urea, estrogen and progesterone in the blood and the level of IGF-I in the follicular liquid of heifers. Twenty seven crossbred heifers were used, in three treatments: 1.2, 1.6 and 2.6% of the live weight of dry matter intake (DMI) daily. The sanguine concentrations of glucose and estrogen were higher in the animals with 1.6% of DMI and the insulin levels were higher in the animals with 2.6% of DMI. There was an increase in the sanguine levels of urea with the increase of the DMI. There was no effect of the DMI on the progesterone levels in the sanguine plasma collected from the jugular. The progesterone levels in caudal vena were higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FSH; Hormones; Intake; Nutrition; Reproduction; Superovulation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100008

Diet and feeding behavior of Kyphosus spp. (Kyphosidae) in a Brazilian subtropical reef
Provedor de dados: 52
Autores: Silvano,Renato Azevedo Matias; Güth,Arthur Ziggiatti.
The present study analyzed and compared diet and feeding behavior (substrate use, position in water column, interactions with other fishes) of Kyphosus spp. (sea chubs) in a Brazilian subtropical reef. Juveniles (&lt; 160 mm) of Kyphosus incisor consumed both algae and invertebrates, which were mainly calanoid copepods. Juvenile and small adults of also observed foraging in the water column. We thus provide the first record of omnivory for Kyphosids in the southwest Atlantic Ocean.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbivory; Kyphosidae; Omnivory; Ontogenetic diet shift; Coastal fishes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500012

Sustainable Management of Keratin Waste Biomass: Applications and Future Perspectives
Provedor de dados: 52
Autores: Sharma,Swati; Gupta,Arun.
Keratin is a durable, fibrous protein which is mainly present in higher vertebrates (mammals, birds and reptiles) and humans epithelial cells. Food industry especially the meat market, slaughter house and wool industry produces million of tons of keratin containing biomass. These industries are constantly growing and the major producers include USA, Brazil and China, account for more than 40 million tons per year. These proteins constitute keratin by-products have from 15 to 18% nitrogen, 2-5% sulphur, 3.20% mineral elements and 1.27% fat and 90% of proteins. The organic waste rich in keratin can be utilized as a natural source using chemical and mechanical methods. The natural keratin obtained by biomass does not contain any harmful chemical and can be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Keratin; Purification; Cosmetics; Cuticle; Coatings; Hygroscopic; Industrial applications.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100701

Production of alcohol by simultaneous saccharification and fermentation of low-grade wheat flour
Provedor de dados: 52
Autores: Neves,Marcos Antonio das; Kimura,Toshinori; Shimizu,Naoto; Shiiba,Kiwamu.
Two samples of low-grade wheat flour, namely low-grade 1 (LG1) and low-grade 2 (LG2), with different carbohydrate and fibrous content, were used as substrates. The samples were liquefied using various concentrations of alpha- or beta-amylase, in order to optimize the production of fermentable sugars; the enzyme alpha-amylase revealed higher performance. After liquefaction, the simultaneous saccharification and fermentation was conducted in a jar fermentor. Amyloglucosidase was used for saccharification, and dry baker's yeast, S. cerevisiae, for fermentation simultaneously. Glucose was consumed promptly in both cases, LG1 and LG2; ethanol production was considerably higher in LG1 (38.6 g/L), compared to LG2 (24.9 g/L). The maximum ATP production was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low grade; Wheat flour; Yeast; Fermentation; Ethanol.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400017

Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
Provedor de dados: 52
Autores: Weibo,Jin; Jin,Liu; Fangli,Wu; Aiguang,Guo.
The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Glutenin; Promoter; Endosperm-specific; Transient expression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002

Sanitation and Drying of Sewage Sludge on Radiant Floors Using Solar Energy and Biogas: Comparison between Different Thicknesses of Deposited Mass
Provedor de dados: 52
Autores: Gontijo,Jéssica Cristine; Wagner,Luiz Gustavo; Souza,Mariana Espíndola de; Possetti,Gustavo Rafael Collere.
ABSTRACT Sewage sludge is a solid waste composed of water, rich in organic matter and nutrients, but concentrator of pathogenic microorganisms. Its use as agricultural fertilizer in the state of Paraná, Brazil is common. However, it is necessary to eliminate or significantly reduce microorganisms by means of a hygiene process. This can be obtained thermally, requiring a source of energy for heat generation. In this sense, a pilot system was constructed consisting of a stainless steel radiant floor, capable of transferring heat to the sewage sludge from coils coupled in base, through which the heated water circulates. The energy comes from solar radiation and is complemented by biogas from the anaerobic sewage treatment. Aiming to investigate the system's...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biogas; Sanitation; Sewage sludge; Solar energy; Thermal drying.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200217

Pollination of soybean (Glycine max L. Merril) by honeybees (Apis mellifera L.)
Provedor de dados: 52
Autores: Chiari,Wainer César; Toledo,Vagner de Alencar Arnaut de; Ruvolo-Takasusuki,Maria Claudia Colla; Oliveira,Arildo José Braz de; Sakaguti,Eduardo Shiguero; Attencia,Valeria Maria; Costa,Fabiana Martins; Mitsui,Marina Hitomi.
This experiment was carried out to evaluate the effect of the honeybee pollination in the production and quality of soybean seeds (Glycine max L. Merril). Seed production was higher (P=0.0001) in covered areas with honeybee colonies (50.64%) and uncovered areas (57.73%) than in covered areas without honeybee colonies. It could be concluded that honeybees were responsible for 95.5% of the pollination accomplished by insects. The pod number in covered treatment with honeybees was 61.38% higher (P=0.0002) than in the covered treatment without honeybees. The average weight of 100 seeds was larger (P=0.0001) in the area covered without honeybees, and reached 17.8 g. The medium content of crude protein in grains was 36.7% and the average oil content was 20.2%....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Soybean; Glycine max; Honeybees; Pollination; Seed production.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100005

Zoophytophagy in predatory Hemiptera
Provedor de dados: 52
Autores: Torres,Jorge Braz; Boyd,David W.
Predaceous hemipteran feeding on different trophic levels have raised questions about their ecology and role in biological control. Therefore, specific adaptations allowing them to simultaneously use plants and animals as sources for their nutritional requirements are important. Enzymatic variability in predatory hemipterans has been suggested as the basic adaptation for convergent or divergent to omnivory. Thus, the salivary enzymatic complexes of predatory hemipterans have been furnished a partial understanding of the mechanisms permitting switching between plant and animal food sources. In this study, a discriminatory analysis was performed to attribute trophic habits to each insect investigated based on the presence and absence of salivary enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding behavior; Salivary glands; Phytophagy; Zoophagy; Biological control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500018

Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensisKSADL127 Isolated from Mangrove Environments of Saudi Arabia
Provedor de dados: 52
Autores: Alarfaj,Abdullah A.; Arshad,Mohammed; Sholkamy,Essam N.; Munusamy,Murugan A.
ABSTRACTPolyhydroxybutyrate (PHB) is a renowned biodegradable plastic that do not release any toxins or residues in the environment like petroleum based plastics. In the present study, 50 bacteria isolated from mangrove niche, Saudi Arabia, were screened for maximum PHB production. All the 50 strains showed positive for PHB production, of which one strain showed maximum of 137 mgL-1. The most PHB accumulated bacterium was selected and identified asBacillus thuringiensis KSADL127, based on phenotypic characterization and 16S rRNA sequence analysis. Characterization of extracted PHB was carried out by FT-IR, NMR, UV spectroscopy, DSC, TGA, and LC-MS, which later confirmed the presence of intracellular accumulated polymer and substantiated as PHB.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangrove environments; Bacillus thuringiensis; Polyhydroxybutyrate (PHB).
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500781

Evaluation of possible failure of the mononuclear phagocyte system after total splenectomy in rats
Provedor de dados: 52
Autores: Marques,Ruy Garcia; Petroianu,Andy; Oliveira,Márcia Betânia Nunes de; Bernardo-Filho,Mário; Portela,Margareth Crisóstomo.
Young and adult Wistar rats were submitted to total splenectomy and compared to animals not submitted to any surgical manipulation in order to evaluate the phagocytic function of spleen. The animals were infected with Escherichia coli labeled with technetium-99m and killed 20 minutes later. Liver, lung, spleen and a blood clot sample were taken. No significant differences were found in the percentage of bacterial radioactivity uptake in mononuclear phagocyte system (MPS) organs in young and adult splenectomized rats. However, phagocytosis index by macrophages of MPS organs was smaller in splenectomized animals than in control group. Splenectomized rats were associated with a higher blood bacterial radioactivity uptake than animals of the control group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spleen; Splenectomy; Technetium-99m; Escherichia coli; Phagocytosis; Mononuclear phagocyte system.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200006

Human FcRn can mediate the transport across intestinal mucosal barrier and prolong the half-life of rabbit IgG in vivo
Provedor de dados: 52
Autores: Pang,Guangchang; Wang,Yufang; Xie,Junbo; Chen,Qingsen; Hu,Zhihe.
FcRn (neonatal Fc receptor) plays an important role in IgG transportation, antigen presentation and signal transmission. In this study, the complement fixation test and flow cytometry test were performed to verify whether the heterologous antibody could be transmitted to the serum or leukocyte with FcγR (Fc gamma receptor) across the intestinal mucosa. The results showed that rabbit anti-bovine IgG could be detected in both the serum and the leukocytes, which indicated that the heterologous antibody could transport across the intestinal mucosa to enter the blood and be effectively delivered to the leukocytes with FcγR. In addition, the results also showed that the rabbit anti-bovine IgG still could be detected in the leukocyte group (P=0.044&lt;0.05) after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FcRn; Heterogeneous IgG transport; Complement fixation test; FcγR; Gastrointestinal barrier.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300387

Yield response to fertilization strategies in no-tillage soybean, corn and common bean crops
Provedor de dados: 52
Autores: Pauletti,Volnei; Serrat,Beatriz Monte; Motta,Antonio Carlos Vargas; Favaretto,Nerilde; Anjos,Adilson dos.
Two research fields were conducted on a Haplohumox from 1998 and to 2005. The study aimed to evaluate the strategies for starter fertilizer application on corn, soybean and common bean yield as well as the accumulation of earlier dry matter for corn in a no-tillage crop rotation system in south of Brazil. There was no increase in the yield of soybean with phosphorus and potassium application for at least seven seasons, while corn and common bean showed increased yields with phosphorus, potassium and nitrogen application. For soybean, corn and common beans in no-tillage systems with high levels of phosphorus and medium of potassium, the starter fertilizer can be applied in any of the methods evaluated and in any of the period considered. The presence or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Starter fertilizer; Application technique; Fertilizer management; Crop rotation; No-till.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300009

Relationship Between the Anxious Symptoms and the Neurotransmitter in Parkinson's Mice with Different Dosages of MPTP
Provedor de dados: 52
Autores: Xia,Yu; Ye,Suzhen; Shi,Jian; Huang,Hanjin.
ABSTRACT This study aims to explore the relationship between the anxious symptoms and the impairment of 5-hydroxytryptamine system in PD mice induced by different dosages of MPTP. The mice from the three model groups, the low-dose, dose and high-dose group, took longer time in the dark box than those in the control group (P&lt;0.05). However, no statistically significant differences were found among the model groups. The number of open arm entry (OE) and the open arm time (OT) were significant lower in the model group than those in the control group in the elevated plus-maze test (P＜0.05). The percentage of OE in modle group was significantly lower compared with the control group (P＜0.05). The concentrations of striatum DA, HVA, 5-HT, and 5-HIAA were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson's disease 1-methyl-4-phenyl-1; 2; 3; 6-tetrahydroPyridine (MPTP) 5-hydroxytryptamine Dopamine Anxiety.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100315

X-ray Microtomography to Quantify Morphological Sandstones Properties
Provedor de dados: 52
Autores: Pires,Luiz Fernando; Melo,Mário Sérgio de; Borges,Jaqueline Aparecida Ribaski; Heck,Richard John; Facin,Paulo Cesar.
Abstract The sandstones of the Furnas Formation in the State of Paraná, south of Brazil, are located in regions with great importance due to the natural heritage found in the rocky forms and associated flora and fauna. This paper presents a detailed analysis of the internal structure of diverse sandstones of the Furnas Formation. X-ray microtomography allowed 3D characterization of the number, pore size and shape distributions of the sandstones. Images with voxel size of 603 µm3 were utilized to evaluate the sandstone’s macroporosity. The main results obtained indicate that higher macroporosities were observed in coarser and some types of silicified sandstones. In general, fine sandstones were characterized by the largest number of pores. As a general...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Image analysis; Porous system; Sandstone structure; 3D analysis; Micromorphology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100605

Structural, hystochemical and cytochemical characteristics of the stigma and style in Passiflora edulis f. flavicarpa (Passifloraceae)
Provedor de dados: 52
Autores: Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Dias,Angelo José Burla; Ribeiro,Beatriz Ferreira; Viana,Alexandre Pio.
Studies were carried out on anatomic aspects of the stigma and style of yellow passionfruit. The stigmatic style consisted of papillae, many layers of sub-epidermal cells and a central transmitting tissue. The stigma was of dry, unbranched, multicellular multiseriate papillate type and the cells were highly vacuolated with walls that were relatively thin consisting of compactly arranged fibrils. The style was solid with a central core of transmitting tissue that had intercellular spaces containing a matrix that included polysaccharidic and lipidic substances. During development, cells of the transmitting tissue were arranged compactly while the mature pistil showed cells with a little more loosely arranged intercellular spaces. Both the mature stigma and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yellow passionfruit; Anatomy; Stigma and style; Histochemistry; Cytochemistry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100011

Non-linear dynamics of chromosome condensation induced by colcemid
Provedor de dados: 52
Autores: Fernandes,Thiago de Salazar e; Silva,Isvânia Maria Serafim da; Moraes,Renato Barros; Aguiar,Leandro Álvaro de Alcântara; Amaral,Ademir; Barbosa,Catão Temístocles; Nogueira,Romildo de Albuquerque.
This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-linear dynamics; Chaos; Chromosome condensation; Colcemid.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100011

Evaluation of the Toxicity of Silver/Silica and Titanium Dioxide Particles in Mammalian Cells
Provedor de dados: 52
Autores: Pittol,Michele; Tomacheski,Daiane; Simões,Douglas Naue; Ribeiro,Vanda Ferreira; Santana,Ruth Marlene Campomanes.
ABSTRACT The increasing concern over the spread of diseases has lead to a high consumption of antimicrobial additives in the medical and industrial fields. Since these particles can lixiviate from loaded materials, the contact between this additive and mammalian cells can occur during manufacture, use and disposal of the products. Silver on fumed silica (AgNP_SiO2) and titanium dioxide (TiO2) can be used as antimicrobial additives that are applied in polymeric formulation. While these additives can inhibit bacteria, fungus and virus proliferation; they may also be harmful to humans. Standard toxicological studies were undertaken using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide), CBPI (cytokinesis-block proliferation index) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Titanium dioxide; Silver; Silica; Toxicology; Mammalian cell.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100318

Açai juice as contrast agent in MRCP exams: qualitative and quantitative image evaluation
Provedor de dados: 52
Autores: Pinho,Kátia Elisa Prus; Pinho,Antonio Carlos; Pisani,Julio Cesar; Goedert,Carlos Alberto; Gusso,Andreia Magri; Gewehr,Pedro Miguel.
Abstract This works aims to assess images obtained with administration of açai juice as compared to a manufactured standard iron oxide-based contrast employed as negative oral agents in Magnetic Resonance Cholangiopancreatography (MRCP), employing qualitative and quantitative evaluation. The research was developed with 64 patients submitted to MRCP exams (on 2 days) in a clinic of Curitiba city (Brazil). First (day 1), a manufactured iron oxide-based contrast (A) was offered and later (day 2), açai juice (contrast B) was given to patients. Radiologists (R1 and R2) evaluated the images, classifying them by a score (1-4). In order to have a quantitative assessment, Image J free software was employed generating plots of gray levels against distance of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Açai contrast agent; Iron oxide contrast; Magnetic resonance cholangiopancreatography; Image quality; Image J.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100600

Random amplified polymorphisms between two South American subspecies of rattlesnakes (Crotalus durissus collilineatus and Crotalus durissus terrificus)
Provedor de dados: 52
Autores: Echeverrigaray,Sergio; Grazziotin,Gilberto; Grazziotin,Felipe; Agostini,Gustavo.
The present work was made to determine the suitability of RAPD analysis for the identification of specimens of two subspecies of South American rattlesnakes (Crotalus durissus terrificus and Crotalus durissus collilineatus). The 11 arbitrary primer sequence tested amplified a total of 161 bands, of which 31% were polymorphics when compared with Crotalus specimens. The combining results from different primers allowed the identification of all the specimens under analysis. Several characteristic bands of each subspecies were identified. Dendrogram, based on RAPD markers, show three groups that corresponded to the subspecies of Crotalus, and to Bothrops jararaca specimen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rattlesnake; RAPD; Genetic variability; Molecular markers.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300014

Decreased mRNA Expression of Cystathionine Gamma lyase and H2S Concentration in Gastric Mucosal Tissue in Diabetic Rats
Provedor de dados: 52
Autores: Mard,Seyyed Ali; Dehkohneh,Kobra Alinejad; Ahangarpour,Akram.
ABSTRACT Background and aim: It is well established that the rate of gastric lesions increases in diabetic rats. Recently, the protective effect of hydrogen sulfide (H2S) in gastric mucosa has been proven. This study aimed to determine the release of H2S and mRNA expression of cystathionine gamma lyase (CSE) in gastric mucosa in alloxan-diabetic rats in response to distention-induced gastric acid secretion. Twenty-four rats were randomly assigned to 4 groups (6 in each). They were the normal-control, distention-control, diabetic-control, and distention-diabetic groups. Under anesthesia, animals underwent a tracheotomy and midline laparotomy. To washout the gastric contents, a catheter was inserted in the stomach through the duodenum. To determine the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric acid secretion; Cystathionine gamma lyase; Hydrogen sulfide; Alloxan-diabetic rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100311

Morpho-anatomical and micromorphometrical evaluations in soybean genotypes during water stress
Provedor de dados: 52
Autores: Stolf,Renata; Medri,Moacyr Eurípedes; Pimenta,José Antônio; Boeger,Maria Regina Torres; Dias,Jaqueline; Lemos,Noélle Giacomini; Oliveira,Maria Cristina Neves de; Brogin,Rodrigo Luís; Yamanaka,Naoki; Neumaier,Norman; Farias,José Renato Bouça; Nepomuceno,Alexandre Lima.
In a greenhouse experiment, morpho-anatomical and micromorphometrical analyses of two soybean cultivars, MG/BR46 (Conquista) and BR16-tolerant and sensitive to drought, respectively-were used to study their water-deficit-tolerance strategies. Drought treatments were applied at reproductive stages from R2 to R7, where evaluations were conducted at 30 days and 45 days after stress started, respectively. The total length of Conquista plants (shoot + root) was greater than of BR16 plants. Pod dry weight was adversely affected due to the lack of moisture, decreasing productivity even of Conquista plants. Both the cultivars had normal development of root hairs; however, it was observed a decrease in the cortex:central cylinder ratio in BR16 stressed for 30 days,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Leguminous; Drought; Root.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600002

Influence of lagoons size and prey availability on the wading birds (Ciconiiformes) in the upper Paraná river floodplain, Brazil
Provedor de dados: 52
Autores: Gimenes,Márcio Rodrigo; Anjos,Luiz dos.
The influence of lagoons size and prey availability on the Ciconiiformes in the upper Paraná river floodplain, Brazil, were analysed. The Ciconiiformes census was conducted quarterly in 2002, simultaneously to the fish census (gill nets and seining nets). There was strong relationship between the lagoons size and birds abundance, but modest relationship of lagoons size with the birds diversity and number of species. The presence of Ciconiiformes in the lagoons was better predicted by the fish density in seining nets than by the fish capture by unit of effort in gill nets. Except in the flood, there was strong relationship between the fishes density and birds relative abundance, and more modest relationship of the fishes density with birds diversity and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wading birds; Ciconiiformes; Prey availability; Lagoons; Paraná river.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400015

Field studies of a Brazilian seahorse population, Hippocampus reidi Ginsburg, 1933
Provedor de dados: 52
Autores: Freret-Meurer,Natalie Villar; Andreata,José Vanderli.
This study was carried out to fill the gaps that remain under Hippocampus reidi biology. Analysis of variations of sex ratio, density, breeding season, distribution and home range of a population of the endangered Brazilian seahorse H. reidi from a rocky shore on Araçatiba beach, Ilha Grande, Brazil were carried out. Araçatiba beach is a tourist Environmental Protected Area, suffering antropic pressure. A fixed population of H. reidi was studied, where al lthe individuals were visually tagged and sex, reproductive state and location on site were identified from December 2002 to November 2004. A total of 20 individuals were visually tagged with a mean density of 0.18 m-2. Sex ratios were skewed, with more females than males. All the males brooded during 13...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seahorse; Hippocampus reidi; Brazil; Natural biology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400012

Phytochrome controls achene germination in Bidens pilosa L. (Asteraceae) by very low fluence response
Provedor de dados: 52
Autores: Amaral-Baroli,Adriana; Takaki,Massanori.
Achene without ornament of the tegument were light insensitive with germination under all tested light conditions. Achene with verrucose ornament of the tegument presented low germination under darkness and high germination under light conditions. By pre-incubation at 36° C for remotion of pre-existing Pfr and by comparison of results of counting of dark germinating achenes at the end of experiment and daily under dim green safe light (0.001mumol m-2 s-1 nm-1) we concluded that germination was controlled by phytochrome through very low fluence response.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bidens pilosa; Phytochrome; VLFR; Achene germination.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200002

Radioguided surgery for breast cancer: preliminary experience in Piaui
Provedor de dados: 52
Autores: Abreu,Benedita; Melo,Nayana; Vieira,Sabas; Abreu,Evandro; Abreu,João Batista de; Pádua Filho,Antonio de.
Intraoperatory detection and excision of sentinel lymph node (SLN), radioguided occult lesion localization (ROLL) and lesional resection, or a combination of these two procedures at one time in a same patient (SNOLL), are techniques that represent, at the moment, a significant advance in breast cancer surgery, procedures in clinical practice being established, as can be seen from the medical literature, as well as in this institution. Thirty-one patients with breast cancer were initially treated with the aid of radioguided surgery. Of those, 20 patients were submitted to sentinel lymph node biopsy, six have undergone only ROLL and five were submitted to a combination of the two techniques.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Sentinel lymph node; Intraoperative probe.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700020

The effects of chronic AC magnetic field on contraction and relaxation of isolated thoracic aorta rings of healthy and diabetic rats
Provedor de dados: 52
Autores: Öcal,Isil; Günay,Ismail.
The aim of in this study was to determine the effect of chronic alternating current (AC) magnetic field on the contraction and relaxation parameters of isolated thoracic aorta rings in healthy and diabetic rats. Sixty rats (Wistar albino spp) weighing between 250-300 g were used. The rats were divided into four groups: 1-Control (C), 2- control + magnetic field (C+MA), 3- experimental diabetic (DIA), 4- experimental diabetic and magnetic field (DIA+MA). Magnetic fields of 5 mT intensity and 50 Hz frequency oriented in the north-south direction was applied to the C+MA and DIA+MA groups for 2 hours each day for one month, after which rats were killed by decapitation and the thoracic aorta dissected. This showed attenuated contraction responses to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetic field; Diabetic rats; Thoracic aorta; Endothelium-depending and endothelium-independing relaxation; Endothelium-derived hyperpolarizing factor (EDHF); Endothelium-derived relaxing factor (EDRF).
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500008

Assessment of Sugarcane Germplasm ( Saccharum spp. complex) Against Red Rot Pathogen Colletotrichum Falcatum
Provedor de dados: 52
Autores: Singh,Atul; Singh,Pratap; Tiwari,A K; Sharma,B L.
ABSTRACT Red rot, caused by Colletotrichum falcatum Went is the most important disease of sugarcane in India inflicting substantial loss to both cane industry and cane growers. To keep in view the importance of red rot disease of sugarcane, 117 accession of sugarcane germplasm including different Saccharum species and Indian and foreign commercial hybrids were tested against red rot with Cf 07, Cf 08 &amp; Cf 09 (national pathotypes) by plug method of inoculation. Out of 117, 6 were found resistant and 12 were moderately resistant against red rot and rest were moderately susceptible/susceptible/highly susceptible. Theses resistance and moderately resistant accession can be further utilize to produce resistance varieties against the most devastating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Germplasm; Resistance; Red rot; Plug method.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100203

Effects of different grinding levels (particle size) of soybean hull on starting pigs performance and digestibility
Provedor de dados: 52
Autores: Moreira,Ivan; Kutschenko,Marianne; Paiano,Diovani; Scapinelo,Cláudio; Murakami,Alice Eiko; Quadros,Arlei Rodrigues Bonet de.
Two experiments were carried out to study the effects of grinding of soybean hulls (SH) on starting pigs (15-30 kg) diets. Experiment I consisted of a digestibility trial with 12 barrows, initial average body weight 21.9±1.29 kg, in which the best digestibility coefficient (DC) of DM and GE was obtained with SH ground through a 2.5mm screen mesh, evaluated for CP and ME, a best DC was attained with 3.0 mm screen mesh. In the Experiment II, five diets with increasing SH (2.5 mm) levels (0, 3, 6, 9 and 12 %) for starting piglets were formulated. A quadratic response in daily weight gain (DWG) occurred, according to SH inclusion. Worst DWG occurred at 7.75 % SH inclusion. Daily feed intake, feed: gain ratio and plasma urea nitrogen were not affected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative feedstuffs; By-product; Nutritional evaluation; Nutrition; Piglets.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500023

Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
Provedor de dados: 52
Autores: Penha,Tânia Regina; Krüger,Ernesto Renato; Thomaz-Soccol,Vanete; Agottani,Jorge Victor Bacila; Itano,Flávio Hiroshi; Troiano,Ludmilla Della Coletta; Brodzinski,Josiane.
The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoclonal antibodies; Bovine immunoglobulin G; Fragment Fc.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014

Metformin protects the skeletal muscle glycogen stores against alterations inherent to functional limitation
Provedor de dados: 52
Autores: Bosi,Paula Lima; Borges,Gabriel Delfino; Durigan,João Luiz Quagliotti; Cancelliero,Karina Maria; Silva,Carlos Alberto da.
The aim of this study was to evaluate the glycogen content (GC) of the rat hind limb muscles submitted to joint immobilization, either associated with metformin treatment (M, 1,4mg.ml-1) or not. In the metformin group, there was a significant increase in the GC (soleus - S 65% , white gastrocnemius - WG 30.5%, red gastrocnemius- RG31.7%, extensor digitorum longus - EDL 44%, tibialis anterior- TA 77.4%). The immobilization significantly reduced the GC (S 31.6%, WG 56.6%, RG 39.1%, ELD 41.7%, TA 45.2%) and weight (S 34.2% and ELD 27%), whereas in the group immobilized with the metformin, there was an increase in the GC of all the muscles (S 177%, WG 290%, RG 172%,ELD 47%, TA 217%), in addition to minimizing the weight loss of S (29.6%) and ELD (27.8%).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle disuse; Metformin; Skeletal muscle; Rehabilitation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200009

Comparison of performance of different algorithms in noisy signals filtering of process in enzymatic hydrolysis of cheese whey
Provedor de dados: 52
Autores: Pinto,Gilson Alexandre; Sousa Júnior,Ruy de; Giordano,Roberto de Campos.
This work presented the results of the implementation of an off-line smoothing algorithm in the monitoring system, for the partial hydrolysis of cheese whey proteins using enzymes, which used penalized least squares. Different algorithms for on-line signals filtering used by the control were also compared: artificial neural networks, moving average and smoothing algorithm.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic proteolysis; PH control; Post-treatment noisy data; On-line noisy signals filtering.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400019

Size at morphological maturity of Ucides cordatus (Linnaeus, 1763) (Brachyura, Ocypodidae) in the Laranjeiras Bay, Southern Brazil
Provedor de dados: 52
Autores: Dalabona,Glaucia; Silva,Jayme de Loyola e; Pinheiro,Marcelo Antonio Amaro.
Morphometry and maturity of Ucides cordatus were analyzed with males and females collected during one year on a monthly basis at Laranjeiras Bay, Paraná State, Brazil. Carapace length, chelipeds propodus length and abdominal width were measured and related to carapace width to verify sexual dimorphism and size at morphological maturity of each sex. Carapace and propodus length of larger and smaller cheliped presented difference between sexes, confirming the use of both as secondary sexual characters. MATURE II program indicated 44mm and 43mm of carapace width to represent the size at sexual maturity of males and females, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproduction; Relative growth; Fishery resource.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100018

Vitamin E and Sodium Selenite Against Mercuric Chloride-Induced Lung Toxicity in the Rats
Provedor de dados: 52
Autores: Celikoglu,Emine; Aslanturk,Ayse; Kalender,Yusuf.
The aim of the present study was to elucidate the possible protective role of vitamin E and / or sodium selenite on mercuric chloride-induced oxidative stress and histopathological changes in the lung tissue of the rats. Adult male albino Wistar rats were exposed to mercuric chloride (1.0 mg/kg day) for four weeks. Treatment with mercuric chloride led to oxidative stress by enhancing MDA level and also decreasing superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPx) and glutathione S transferaz (GST) activities. However, mercuric chloride exposure resulted in histopathological changes in the lung tissue in the rats. MDA level and SOD, CAT GPx and GST activities and histopathological changes modulated in concomitantly supplementation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercuric chloride; Sodium selenite; Vitamin E; Lung toxicity; Oxidative stress; Histopathology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400587

In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata)
Provedor de dados: 52
Autores: Balakrishna,Aparna.
The aim of this work was to study the probiotic-related characteristics of four strains of bacteria isolated from the normal flora of the guppy, Poecilia reticulata. In vitro results showed that the strains, namely, MBTU_PB1, MBTU_PB2, MBTU_PB3 and MBTU_PB4 had higher adhesion abilities than the tested indicator strains. However, an association between the cell-surface hydrophobicity and the ability to adhere to the intestinal mucus was not observed for these strains. Further, the selected strains were strongly autoaggregating (autoaggregation percentage ≥ 80) and also showed strain-specific coaggregation abilities with the tested indicator strains.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotics; Adhesion; Aggreggation; Hydrophobicity; Poecilia reticulata.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500010

Enhanced biosynthesis of quercetin occurs as A photoprotective measure in Lycopersicon esculentum mill. under Acute UV-B exposure
Provedor de dados: 52
Autores: Shourie,Abhilasha; Tomar,Pushpa; Srivastava,Deepika; Chauhan,Rahul.
Lycopersicon esculentum respond to UV-B by enhanced synthesis of flavonoid quercetin, a strong antioxidant that helps the plants to well acclimatize to UV-B stress. Three weeks old plants of L. esculentum were subjected to acute UV-B irradiation for 20, 40 and 60 minutes daily until 28 days and analyzed for the morphological and biochemical changes. UV-B exposure for 40 and 60 minutes considerably affected the growth and biomass of L. esculentum. The leaves were deformed, developed chlorosis and abscised early as compared to the unexposed plants. Biomass declined by 35% and total chlorophyll decreased by 24.7% due to disintegration of chloroplasts. Enhancement was seen in the content of carotenoids, anthocyanins and total flavonoids by 15, 33.3 and 22.8%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: UV-B; Lycopersicon esculentum; Quercetin; Biomass; Pigments; Anthocyanins.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300002

Association Between Exercise and Treatment with Liraglutide in Obese Rats by Cafeteria Diet
Provedor de dados: 52
Autores: Didek,Daiane; Cordeiro,Maiara Mikuska; Xavier,João Lucas de Paula; Ribeiro,Paulo Roberto; Rentz,Thiago; Franco,Gilson Cesar Nobre; Kanunfre,Carla Cristine; Emilio,Henriette Rosa de Oliveira; Scomparin,Dionízia Xavier.
Abstract The objective of this study was to evaluate the effect of liraglutide, an analog of glucagon-like peptide-1 (GLP-1) in association with physical exercise, on the metabolic and biochemical parameters of rats induced to obesity with a cafeteria diet. Male Wistar rats, aged 21 days, were randomly divided into: Controls (CON) receiving standard feed and water ad libitum; and obese (OBESE) receiving cafeteria diet ad libitum, added to the standard diet. Groups were then subdivided into: Liraglutide animals that received subcutaneous injections of liraglutide from 80 to 90 days of life; exercised (EXE) animals submitted to swimming sessions, three days a week (15 min); and liraglutide + EXE animals that received liraglutide in association with physical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Liraglutide; GLP-1; Metabolic syndrome; Exercise.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100305

Growth of fresh-water prawn Macrobrachium tenellum (Smith, 1871) juveniles fed isoproteic diets substituting fish meal by soya bean meal
Provedor de dados: 52
Autores: García-Ulloa Gomez,Manuel; López-Aceves,Luis Alberto; Ponce-Palafox,Jesús Trinidad; Rodríguez-González,Hervey; Arredondo-Figueroa,José Luis.
Growth parameters (standard length, weight, specific growth rate and daily weight gain) of prawn Macrobrachium tenellum juveniles fed 40% crude protein isoproteic diets substituting fish meal with soya bean meal at various levels (20, 40, 60, 80 and 100%) were evaluated for 45 days under laboratory conditions. Experimental diets were compared with a 100% fish meal based diet. Total survival was recorded for all the treatments at the end of the experiment. There were no significant differences (p &gt; 0.05) for all the growth parameters among the dietary treatments. The initial mean weight (0.36 ± 0.10 g) increased almost three times (1.00 ± 0.13 g) after 45 days. The final specific growth fluctuated from 1.82% BW/d for the 60% soya bean meal inclusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Fresh water prawn; Macrobrachium tenellum; Isoproteic diets; Soya bean meal; Aquaculture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100008

Floral biology and behavior of Africanized honeybees Apis mellifera in soybean (Glycine max L. Merril)
Provedor de dados: 52
Autores: Chiari,Wainer César; Toledo,Vagner de Alencar Arnaut de; Ruvolo-Takasusuki,Maria Claudia Colla; Attencia,Valeria Maria; Costa,Fabiana Martins; Kotaka,Carolina Satie; Sakaguti,Eduardo Shiguero; Magalhães,Helida Regina.
This research was carried out to evaluate the pollination by Africanized honeybees Apis mellifera, the floral biology and to observe the hoarding behavior in the soybean flowers (Glycine max Merril), var. BRS-133. The treatments were constituted of demarcated areas for free visitation of insects, covered areas by cages with a honeybee colony (A. mellifera) and also covered areas by cage without insects visitation. All areas had 24 m² (4m x 6m). The soybean flowers stayed open for a larger time (82.82 ± 3.48 hours) in covered area without honeybees. The stigma of the flowers was also more receptive (P=0.0021) in covered area without honeybees (87.3 ± 33.0%) and at 10:42 o'clock was the schedule of greater receptivity. The pollen stayed viable in all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Africanized honeybee; Apis mellifera; Soybean; Glycine max; Pollination; Floral biology; Honeybee behavior.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300006

ICP OES Determination of Contaminant Elements Leached from Food Packaging Films
Provedor de dados: 52
Autores: Santos,Éder José dos; Oliveira,Fernanda Mara Pimentel; Herrmann,Amanda Beatriz; Sturgeon,Ralph Edward.
ABSTRACT  Determination of potential contaminants elements in food packing films arising from contact with acidic aqueous foods was undertaken by inductively coupled plasma optical emission spectrometry (ICP OES) in accordance with DIN EN 1186-1. Test sections from plastic films were totally immersed in 3% w/v CH3COOH used as the food simulant. Testing was conducted under three conditions: (1) 10 days at 40 ºC; (2) 30 min at 70 ºC and 10 days at 40 ºC; and (3) 30 min at 100 ºC and 10 days at 40 ºC. These time and temperature conditions were considered to be the most severe situations likely to be encountered in practice. Several different containers were investigated, including a borosilicate glass beaker, a glass bottle used for food canning, as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food packaging; ICP OES; Contaminant elements.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100424

Food Preference of Cavia magna (Rodentia: Caviidae) on a Subtropical Island in Southern Brazil
Provedor de dados: 52
Autores: Veiga,Kelen Rodrigues da; Hefler,Sônia Marisa; Colares,Elton Pinto; Colares,Ioni Gonçalves.
Abstract This study aimed to link the plants consumed by Cavia magna and their availability in the environment, (Ilha dos Marinheiros) determining its food preference. In the environment, 96 species were recorded. Twenty-four plant species were identified in the fecal samples. There wasn’t significant difference in the composition of plant species among seasons, although the variation was significant between the island’s northern and southern areas concerning the fecal samples. Mantel test’s results supported that the availability of vegetation has significant influence on the species consumed. Being that the most consumed species (present on the feces) were distant to their distribution areas, we propose that C. magna of Ilha dos Marinheiros actively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caviidae; Estuary; Herbivory; Rodent; Vegetation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100418

Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues
Provedor de dados: 52
Autores: Alberton,Luiz Romulo; Vandenberghe,Luciana Porto de Souza; Assmann,Ricardo; Fendrich,Ricardo Cancio; Rodriguéz-León,José; Soccol,Carlos Ricardo.
The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomyces; Xylanase; Submerged fermentation; Solid-state agro-industrial fermentation; Residues.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022

Enhanced lipase recovery through RSM integrated differential evolutionary approach from the fermented biomass
Provedor de dados: 52
Autores: Garlapati,Vijay Kumar; Vundavilli,Pandu Ranga; Banerjee,Rintu.
The aim of this work was to apply a modeling integrated optimisation approach for a complex, highly nonlinear system for an extracellular lipase extraction process. The model was developed using mutation, crossover and selection variables of Differential Evolution (DE) based on central composite design of Response Surface Methodology. The experimentally validated model was optimized by DE, a robust evolutionary optimization tool. A maximum lipase activity of 134.13 U/gds (more than 36.28 U/gds compared to one variable at a time approach) was observed with the DE-stated optimum values of 25.01% dimethyl sulfoxide concentration, 40 mM buffer, 128.52 min soaking time and 35ºC with the DE control parameters, namely number of population, generations, crossover...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Modeling; Optimization; Lipase extraction; Response surface methodology; Differential evolution; Solid state fermentation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500001

Extract of the Bark of Bathysa cuspidataAttenuates the Development of Chemically-Induced Preneoplastic Colorectal Lesions in Rats
Provedor de dados: 52
Autores: Rosa,Damiana Diniz; Reis,Sandra Aparecida dos; Siqueira,Nathane Pais; Gonçalves,Reggiani Vilela; Machado,Alessandra Barbosa Ferreira; Tafuri,Natália Filardis; Leite,João Paulo Viana; Matta,Sérgio Luís Pinto da; Peluzio,Maria do Carmo Gouveia.
ABSTRACTThe aim of this study was to investigate the effect of the bark extractBathysa cuspidata on chemically induced preneoplastic colorectal lesions in Wistar rats. Forty male rats were randomly divided into four groups (n = 10 each): saline (control group, oral administration of saline solution 0.9%); dimethylsulfoxide (DMSO, vehicle control), B200 (treated with 200 mg/kg bark extract ofB. cuspidata), and B400 (treated with 400 mg/kg bark extract ofB. cuspidata). Administration of treatments was carried out by the gavage. The animals received four subcutaneous injections of 1,2-dimethylhydrazine (DMH, 40 mg/kg) in the initial two weeks of the experiment to induce preneoplastic colorectal lesions. After 15 weeks, the animals were euthanized and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bathysa cuspidata; Animal model; Oxidative stress; Aberrant crypt foci.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500732

Consortium of Microalgae for Tannery Effluent Treatment
Provedor de dados: 52
Autores: Pena,Aline de Cassia Campos; Bertoldi,Crislaine Fabiana; Fontoura,Juliana Tolfo da; Trierweiler,Luciane Ferreira; Gutterres,Mariliz.
Abstract Wastewater generated in tanneries have essential elements for microalgae growth, but it has also some toxic compounds that may hinder or restrain the growth of microalgae in this environment. This work tested microalgae consortium growth originating from a deactivated effluent treatment decanter of a complete tannery (beamhouse to finished leather) for the treatment of wastewater of a tannery processing wet-blue leather to finished leather. It was initially evaluated the growth of the microalgae consortium in the three effluents diluted in 50% distilled water: raw effluent (50RE50W), effluent after primary coagulation/flocculation (50PE50W), and effluent after primary and secondary biological treatment (50BE50W). After 16 days of cultivation, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalgae consortium; Tannery; Effluent treatment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100701

A Novel Run-length based wavelet features for Screening Thyroid Nodule Malignancy
Provedor de dados: 52
Autores: Haji,Salih Omer; Yousif,Raghad Zuhair.
Abstract: Thyroid nodules are cell growths in the thyroid which might be for in one of two categories benign or malignant. Nodular thyroid disease is common and because of the associated risk of malignancy and hyper-function; these nodules have to be examined thoroughly. Hence diagnosing thyroid nodule malignancy in the early stage can mitigate the possibility of death. This paper presents an intelligent thyroid nodules malignancy diagnosis using texture information in run-length matrix derived from 2- level 2D wavelet transform bands (approximation and details). In this work, ANOVA test has been used to for feature selection to reduce for feature selection about 45 run-length features with and without wavelet generated, before feeding those features which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid Nodule; SVM and DT classifiers; Computer-aided iagnosis; Feature extraction; Run-Length Matrix.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100608

Hunter color dimensions, sugar content and volatile compounds in pasteurized yellow passion fruit juice (Passiflora edulis var. flavicarpa) during storage
Provedor de dados: 52
Autores: Sandi,Delcio; Chaves,José Benício Paes; Sousa,Antônio Carlos Gomes de; Parreiras,June Ferreira Maia; Silva,Marco Túlio Coelho da; Constant,Patrícia Beltrão Lessa.
Changes in Hunter L, a and b values, glucose, fructose and sucrose contents, concentration of four volatile compounds (ethyl butirate, ethyl caproate, hexyl butirate and hexyl caproate) and furfural, were studied in yellow passion fruit juice (Passiflora edulis var. flavicarpa) pasteurized at 75ºC/60s, 80ºC/41s or 85ºC/27s, during storage at room temperature (25±5ºC) and refrigeration (5±1ºC) for 120 days. While the sucrose content decreased, the glucose and fructose contents increased significantly over storage time. The Hunter L and b values behaved similarly, with a tendency to decrease over time, inversely to Hunter a value. Volatile compound concentrations also decreased over time, inversely to the furfural content. Pasteurization at 85ºC/27s resulted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yellow passion fruit juice; Passion fruit processing; Storage; Physical and chemical analysis; Volatile compounds.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200011

Estudo de parâmetros bioquímicos de éguas gestantes, Puro Sangue Árabe
Provedor de dados: 52
Autores: Unanian,Maria Marina; Silva,Antonio Emidio D. Feliciano; Manzano,Airton.
Em éguas Puro Sangue Árabe manejadas em pastagem de Coast-cross (Cynodon dactylon) foram determinados vários parâmetros bioquímicos no período inicial e final da gestação. Para o estudo foram coletados sangue, urina e fezes, alem de feitas medições corporais. As éguas ganharam, em média, 69,4 kg entre o inicio e final da prenhez. O nível de proteína total sangüínea aumentou (P&lt;0,05) do primeiro ao último mês de gestação, enquanto que o de albumina diminuiu. A proporção albumina : proteína manteve-se constante, mostrando que a dieta protéica destes animais era equilibrada. Os níveis de cálcio, fósforo, magnésio, cobre e zinco no soro sangüíneo, e os de proteína total, glicose, creatinina e uréia da urina, não variaram durante a prenhez. Os níveis de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arab mares; Biochemical parameters; Gestation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100015

Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians
Provedor de dados: 52
Autores: Quoirin,Marguerite; Bona,Cleusa; Souza,Elizandra F. de; Schwartsburd,Pedro B.
Plantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Black wattle; Legume tree; Micropropagation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002

Sensitivity to halothane and its relationship to the development of PSE (Pale, Soft, Exudative) meat in female lineage broilers
Provedor de dados: 52
Autores: Marchi,Denis Fabrício; Trindade,Marco Antônio; Oba,Alexandre; Soares,Adriana Lourenço; Ida,Elza Iouko; Ferraz,José Bento Sterman; Michelan Filho,Tércio; Ziober,Iris Lamberti; Shimokomaki,Massami.
This work aimed to evaluate female lineage broilers for halothane sensitivity and for their susceptibility to the subsequent development of PSE meat. The halothane test was carried out in an anesthetic chamber with 3.0% halothane. The unconscious birds were examined for leg muscle rigidity. If one or both legs became extended and rigid, the birds were classified as halothane sensitive (HAL+), while unresponsive birds were classified as halothane negative (HAL-). The results showed that of 298 birds aged 42 days old, 95.6% were HAL- and 4.4% were HAL+. A sample of pectoralis major muscle was collected from HAL- (n=105) and HAL+ (n=13) birds. The pH and breast fillet color were determined at 4ºC, 24 hours post-mortem. Interestingly, only 2.5% of HAL+ birds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental factors; Pectoralis major; Meat color abnormality.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700028

Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells
Provedor de dados: 52
Autores: Carneiro,Marcella Lemos Brettas; Porfírio,Elaine Paulucio; Otake,Andréia Hanada; Chammas,Roger; Báo,Sônia Nair; Guillo,Lidia Andreu.
The aim of this work was to study the effect of curcumin on cell cycle in the human SK-MEL-37 melanoma cell line. In addition, morphological and structural analyses were also performed. Flow cytometric analysis showed a G0/G1 arrest at 5 µM after 24 h exposure and a concentration-dependent increase in the proportion of sub-G0 hypodiploid cells. Typical apoptotic events were also observed by the fluorescence microscopy, transmission and scanning electronic microscopy. Loss of mitochondrial membrane potential was not detected. Results suggested that curcumin could arrest human melanoma cells at G0/G1 phase and induce a mitochondrial-independent apoptotic pathway.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin; Melanoma cells; Apoptosis; Cell cycle.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200013

Brown Flaxseed Prevents DNA Damage Induced by 1,2-Dimethylhydrazine in a Pre-Clinical Model
Provedor de dados: 52
Autores: Pamplona-Silva,Maria Tereza; Morandi,Walter Vinicius; Bernardi,Luana; Tura,Bethânia Borges; Oliveira,Diego Duarte Marques de; Antoniolli-Silva,Andreia Conceição Milan Brochado; Mauro,Mariana de Oliveira; Oliveira,Rodrigo.
ABSTRACT The study evaluated the effects of brown flaxseed supplementation in natura on the prevention of DNA damage induced by 1,2-dimethylhydrazine (DMH) in vivo. The experimental groups were Negative and Positive Controls and the protocols of Pre-treatment, Simultaneous, Post-treatment, Pre+continuous in relation to the supplementation of brown flaxseed and administration with carcinogenic compound. The results showed that brown flaxseed supplementation does not cause genomic and genetic damage. In addition, brown flaxseed showed a chemopreventive food that reduced the damages assessed by the comet assay up to 94.07x and the damages assessed by the micronucleus assay up to 91.88x. Brown flaxseed supplementation also increased the frequency of monocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flaxseed; Antigenotoxicity; Chemoprevention; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100316

The control of documents maintained in the electronic media and the requirements of NBR ISO/IEC 17025 and adequacy for PNQ
Provedor de dados: 52
Autores: Castro,Anselmo Ferreira de; Silva,Glória Maria Pereira da; Costa,Stella Regina Reis da; Santos,Sílvio Francisco dos.
During the assessments carried out by the authors at the diverse laboratories pertaining to the network of accredited laboratories - known as the Brazilian Calibration Network (RBC) - the authors verified the difficulties that these laboratories had in meeting the requirements of NBR ISO/IEC 17025:2001 (Standard), related to the maintenance of documents in the electronic media. More and more laboratories are substituting traditional control with electronic document control, which allows for more agility in the recovery of information. These laboratories implement policies and procedures; however, they still feel insecure, in some way, as to how to meet all of these requirements, thereby giving rise to difficulties in the implementation of such. The aim of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NBR ISO/IEC 17025; PNQ; Computerized systems; Documents.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200018

Two decades of radiological accidents direct causes, roots causes and consequences
Provedor de dados: 52
Autores: Rozental,Jose de Julio.
Practically all Countries utilize radioisotopes in medicine, industry, agriculture and research. The extent to which ionizing radiation practices are employed varies considerably, depending largely upon social and economic conditions and the level of technical skills available in the country. An overview of the majority of practices and the associated hazards will be found in the &lt;A HREF="#tabela4"&gt;Table IV&lt;/A&gt; to &lt;A HREF="#tabela7"&gt;VII&lt;/A&gt; of this document. The practices in normal and abnormal operating conditions should follow the basic principles of radiation protection and the Safety of Radiation Sources, considering the IAEA Radiation Protection and the Safety of Radiation Sources, Safety Series 120 and the IAEA Recommendation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiological accidents; Direct causes; Roots causes; Radiation protection; Safety of Radiation Sources; Safety Culture.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500018

Influence of static magnetic fields on S. cerevisae biomass growth
Provedor de dados: 52
Autores: Muniz,João B.; Marcelino,Milton; Motta,Mauricio da; Schuler,Alexandre; Motta,Mauricy Alves da.
Biomass growth of Saccharomyces cerevisiae DAUFPE-1012 was studied in eight batch fermentations exposed to steady magnetic fields (SMF) running at 23ºC (± 1ºC), for 24 h in a double cylindrical tube reactor with synchronic agitation. For every batch, one tube was exposed to 220mT flow intensity SMF, produced by NdFeB rod magnets attached diametrically opposed (N to S) magnets on one tube. In the other tube, without magnets, the fermentation occurred in the same conditions. The biomass growth in culture (yeast extract + glucose 2%) was monitored by spectrometry to obtain the absorbance and later, the corresponding cell dry weight. The culture glucose concentration was monitored every two hours so as the pH, which was maintained between 4 and 5. As a result,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S. cerevisiae; Biomass; Magnetic fields; Fermentation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300017

Cytotoxicity and genotoxicity of untreated hospital effluents
Provedor de dados: 52
Autores: Ortolan,Maria da Graça Silva; Ayub,Marco Antônio Záchia.
Untreated hospital effluent samples were tested for cytotoxic and genotoxicity in order to access whether they presented any serious health hazards. Results of umuC test suggested that the effluent from a large, general hospital could be considered weakly genotoxic. Comparatively, effluents from inpatient units presented higher frequency of genotoxicity than those coming from laboratory facilities. Although no cytotoxic activity was detected in laboratory effluent samples, cytotoxicity appeared to be an important problem of effluents from inpatient units.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Cytotoxicity; Untreated hospital effluents; Water sources contamination.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400009

Qualitative-feed-restricted heavy swine: meat quality and morpho-histochemical characteristics of muscle fibers
Provedor de dados: 52
Autores: Fraga,Alessandro Luís; Thomaz,Maria Cristina; Kronka,Rodolfo Nascimento; Budiño,Fábio Enrique Lemos; Huaynate,Rizal Alcides Robles; Scandolera,Antônio João; Ruiz,Urbano dos Santos; D'Angelis,Flora Helena de Freitas.
To evaluate the effect of different levels of qualitative feed restriction (0, 5, 10, 15 and 20%) on pork quality and muscle morpho-histochemical characteristics, 60 castrated male swines were used. Ten animals were slaughtered at 89 kg BW. Other 50 pigs were fed experimental diets and slaughtered at 128 kg BW. Qualitative restriction increased pH45, and water holding capacity, and reduced the redness, yellowness and cholesterol concentrations of Longissimus lumborum. Quadratic tendency for oleic acid contents of loin and fiber cross-section area of Multifidus dorsi (P=0.08) were observed, with maximum point at 11-12% of restriction. No effect (P&gt;0.1) on percentage of M. dorsi fibers SO, FG and FOG was found. The meat from 128-kg-BW pig showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Color; Fatty acid profile; Muscle fiber; Nutritional value; Tenderness.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500012

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.
Provedor de dados: 52
Autores: Bajpai,Anju; Kalim,Shahina; Chandra,Ramesh; Kamle,Madhu.
A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction was obtained on MS basal medium supplemented with 2.0 mgL-1 2, 4-D, 400 mgL-1 L-glutamine, 6% sucrose and 500 mgL-1 Malt extract. Following SE induction different developmental stages of somatic embryos (Globular, heart-shaped, torpedo, cotyledonary) was directly obtained and further recurrent embryogenesis also obtained upon prolonged incubation in induction media. Addition of polyethylene glycol (50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryogenesis; Zygotic embryo; Recurrent; Maturation; Acclimatization; Induction; Regeneration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100412

Immobilization of Bacillus megaterium MTCC 2444 by Ca-alginate entrapment method for enhanced alkaline protease production
Provedor de dados: 52
Autores: Mrudula,Soma; Shyam,Nidhi.
Optimization of culture conditions and immobilization parameters for alkaline protease production was carried out by employing Bacillus megaterium MTCC2444. The partially purified enzyme was tested for its stability in the presence of oxidants, surfactants and commercial detergents. The optimum temperature, pH, incubation time and inoculum size were 55 ºC, 11, 48 h, 1 %, respectively. Calcium alginate was used as the immobilization matrix and the effects of gel concentration, bead size, age of immobilized cells, solidification period and initial biomass concentration on alkaline protease production and cell leakage were investigated. The results indicated that the immobilization was most effective with 4 % gel concentration, bead size of 3 mm, 24 h aged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optimization; Immobilized cells; Cell leakage; Detergent; Oxidant stability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100017

Padrão de vascularização do rizoma de gengibre (Zingiber Officinale R)
Provedor de dados: 52
Autores: Santos,Gedir de Oliveira; Silva,Eldo A. Monteiro da.
The vascular system in the rhizome of ginger (Zingiber officinale R.) is arranged in three distinct systems: an inner system that form the principal vascular system of the rhizome, an intermediated system originating a thin camada of anastomosing vascular strands, having direct contact with the inner system and an outer system free of the anastomosis with the another vascular systems. The intermediated and inner system are associated with the root insertion and whole systems are relacioned with the formation of bud adventicious maintaing the same pattern of the rhizome and the communication between the outer and inner system occurs through the bridging to inner system leaf traces.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zingiberaceae; Rizoma; Zingiber; Vascularização.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300012

Slow-release urea in supplement fed to beef steers
Provedor de dados: 52
Autores: Gonçalves,Ana Paula; Nascimento,Carolina Fernanda Moysés do; Ferreira,Fernanda Altieri; Gomes,Rodrigo da Costa; Manella,Marcelo de Queiroz; Marino,Carolina Tobias; Demarchi,João José Assumpção de Abreu; Rodrigues,Paulo Henrique Mazza.
Replacing regular urea (RU) by slow-release urea (SRU) at two levels of non-protein nitrogen (NPN) in concentrate, offered with low-quality roughage, was evaluated in beef steers on dry matter intake (DMI), ruminal fermentation parameters, plasma urea nitrogen (PUN), total tract apparent digestibility of diets and in situ degradability of nitrogen sources. Eight ruminally cannulated steers were allocated into two 4x4 Latin squares, totalizing four treatments: 40 NPN/0 SRU: 40% of concentrate crude protein (CP) as NPN, resulting from 0% of SRU and 100% of RU; 40 NPN/50 SRU: 40% of concentrate CP as NPN, resulting from 50% of SRU and 50% of RU; 40 NPN/100 SRU: 40% of concentrate CP as NPN, resulting from 100% of SRU and 0% of RU; 80 NPN/100 SRU: 80% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Controlled release; Feed intake; Non-protein nitrogen; Short chain fatty acids.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100022

Environmental analysis and zoning for an urban park management purpose
Provedor de dados: 52
Autores: Zanin,Elisabete Maria; Santos,José Eduardo dos; Henke-Oliveira,Carlos.
Environmental analysis and zoning were carried out for management purpose of the Longines Malinowski Municipal Park (LMMP). With an area of 24.85 ha, this urban park includes a patch of sub-tropical humid forest, completely surrounded by residential and commercial areas in the City of Erechim, Rio Grande do Sul State,Brazil. Landscape features derived from aerophotogrametric maps, based on the use of Geographical Information Systems (GIS-IDRISI), resulted in several thematic maps, through which a proposal of environmental zoning was perfomed, observing the definition of five zones of use. The environmental diagnosis identified several types of activities potentially harmful to the biodiversity of the LMMP and city's environmental quality. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urban green area; Quality of life; GIS.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500018

Coexpression of rumen fungal xylanase and bifunctional cellulase genes in Escherichia coli
Provedor de dados: 52
Autores: Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil.
ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407

Sentinel lymphnode in breast cancer: an experience with 53 cases
Provedor de dados: 52
Autores: Abreu,Benedita Andrade Leal de; Melo,Nayana Alves de Brito; Abreu,Evandro Leal de; Vieira,Carlos Sabas; Abreu,Everardo Leal de.
The value of sentinel lymphnode biopsy or selective linfadenectomy in assessing axillary status in breast carcinoma is well established in medical literature. This work presents a briefy study from our preliminary experience with 53 patients submitted to pre-surgical limphoscintigraphy and intraoperatory detection of sentinel lymphnode and compares our records with those of current medical literature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Sentinel lymphnode; Intraoperative probe.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600006

Antibody conjugate radioimmunotherapy of superficial bladder cancer
Provedor de dados: 52
Autores: Perkins,Alan; Hopper,Melanie; Murray,Andrea; Frier,Malcolm; Bishop,Mike.
The administration of antibody conjugates for cancer therapy is now proving to be of clinical value. We are currently undertaking a programme of clinical studies using the monoclonal antibody C595 (IgG3) which reacts with the MUC1 glycoprotein antigen that is aberrantly expressed in a high proportion of bladder tumours. Radioimmunoconjugates of the C595 antibody have been produced with high radiolabelling efficiency and immunoreactivity using Tc-99m and In-111 for diagnostic imaging, and disease staging and the cytotoxic radionuclides Cu-67 and Re-188 for therapy of superficial bladder cancer. A Phase I/II therapeutic trail involving the intravesical administration of antibody directly into the bladder has now begun.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibody; Radioimmunotherapy; Bladder cancer.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500012

Population structure of two Astyanax Baird &amp;amp; Girard, 1854 (Teleostei, Characidae) species from upper Paraguaçu river
Provedor de dados: 52
Autores: Santos,Alexandre Clistenes de Alcântara; Novaes,José Luís Costa.
The fishes of the family Characidae are among the main species of fresh water fishes in South America. In this work, some aspects of the population structure of two Astyanax species were assessed. The fishes were caught in Santo Antônio and São José rivers, in the Paraguaçu basin, by standardized monthly samplings adapted to the microhabitats. Astyanax sp. b (São José river) presented larger size than Astyanax sp. a (Santo Antônio river). The length-weight relationship showed positive allometric growth for Astyanax sp. a, and negative for Astyanax sp. b. Both the species showed a long reproductive period, with higher activities in dry period.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax; Characidae; Chapada Diamantina; Paraguaçu River; Population structure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100013

Development and Psychometric Properties of TQWL-42 to Measure the Quality of Work Life
Provedor de dados: 52
Autores: Pedroso,Bruno; Pilatti,Luiz Alberto; Gutierrez,Gustavo Luis; Picinin,Claudia Tania.
Abstract The objective of this study was to construct and validate an instrument that evaluates the Quality of Work Life (QWL) globally, following the WHOQOL instruments and founded in QWL's classical theoretical models, directed to the contemporary society. The psychometric properties tested were content validity, face validity, internal consistency, criterion validity, concurrent validity and test-retest reliability in an application to 283 individuals. The final version of the instrument is constituted by 47 questions, being five to sample knowledge and 42 divided in five spheres that contemplate QWL's dimensions. For the results calculation a SPSS syntax and a tool in Microsoft Excel that perform the automated calculation after the data tabulation were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality of work life; Measurement instrument; Psychometric properties.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100601

Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts
Provedor de dados: 52
Autores: Meneghin,Maria Cristina; Reis,Vanda Renata; Ceccato-Antonini,Sandra Regina.
The aim of this work was to study the in vitro antibacterial activity possessed by killer yeast strains against bacteria contaminating alcoholic fermentation (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), in cell X cell and cell X crude toxin preparations. The bacteria were not inhibited by any S. cerevisiae killer strains (5 out of 11). The inhibition caused by two crude toxin preparations (Trichosporon figueirae and Candida sp) against L. plantarum was surprisingly high but not in the same extent for B. subtilis, especially with three killer strains (Candida glabrata, Pichia anomala and Candida sp). L. mesenteroides and L. fermentum strains were neither inhibited in cell X cell nor crude toxin X cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Killer yeasts; Alcohol; Fermentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500006

Mixed Cultures Fermentation for the Production of Poly-ß-hydroxybutyrate
Provedor de dados: 52
Autores: Shalin,Thomas; Sindhu,Raveendran; Binod,Parameswaran; Soccol,Carlos Ricardo; Pandey,Ashok.
Poly-ß-hydroxybutyrate (PHB) is a biodegradable intracellular microbial product produced by many bacteria and it is comparable to some of the petrochemical derived thermoplastics such as polypropylene. One of the main barriers for the commercial exploitation is the high cost of the substrate for the production of biopolymer. The utilization of mixed microbial cultures facilitates the use of complex substrates thereby reducing the cost of PHB production. In the present study, mixed culture systems were evaluated for PHB production. Bacillus firmus NII 0830 was used for the production of PHB since it accumulates a large amount of PHB and a second organism Lactobacillus delbrueckii NII 0925 was used to provide lactic acid. FTIR and 1H NMR analyses revealed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poly-3-hydroxybutyrate; Biopolymer; Mixed culture; Bacillus firmus; Lactobacillus delbrueckii; Biodegradation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500644

Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
Provedor de dados: 52
Autores: Morais,Glaucia Almeida de; Takaki,Massanori.
Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Β-amylase; Determination; Selective inactivation; Phaseolus vulgaris.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003

Evaluation of Water Variables in No-Take Zone of Ustica Marine Protected Area (Southern Tyrrhenian Sea)
Provedor de dados: 52
Autores: Sanfilippo,Marilena; Capillo,Gioele; Spanò,Nunziacarla; Manganaro,Antonio.
ABSTRACT In this research, the seasonal fluctuations of the main water variables in the no-take area (A zone) of the Ustica MPA were investigated. This study aims to monitor the water quality of the Ustica MPA, to analyze all the hydrological parameters useful to the physical-chemical characterization of water and to use TRIX index to characterize the trophic level of the coastal marine area. The final results shown that Ustica is an area of relevant environmental and ecological value and is a good example of a Marine Protected Area, which is not excessively affected by the human presence even during the touristic season. This study represents the first records based on well-organized protocol in order to assess water column characteristics of the studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ustica Island; MPA; No-take zone; Water quality; Water variables.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100705

Rats offspring exposed to Ipomoea Carnea and handling during gestation: neurochemical evaluation
Provedor de dados: 52
Autores: Schwarz,Aline; Hosomi,Rosana Zoriki; Flório,Jorge Camilo; Bernardi,Maria Martha; Górniak,Silvana Lima; Spinosa,Helenice Souza.
The present study evaluated the central monoamine levels of male and female adult rat offspring exposed orally by gavage to 0.0, 0.7, 3.0 and 15.0 mg/kg I. carnea aqueous extract daily, from gestation day (GD) 5 to GD 21. Several alterations in the monoamine activity systems were observed. However, the major differences were noted between the 0.0 mg/kg and the no gavage control groups, showing that alterations showing that alterations were not due to the alterations to the aqueous extract. The control data showed that gavage and handling of dams were stressful enough to produce a significant decline in 3,4-dihydroxyphenylacetic acid (DOPAC) and an increase in vanilmandelic acid (VMA), indicating decreased dopamine (DA) and enhanced norepinephrine (NE)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipomoea carnea; Neurotransmitter; Perinate; Rats; Stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300009

Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)
Provedor de dados: 52
Autores: Ninhaus-Silveira,Alexandre; Foresti,Fausto; Veríssimo-Silveira,Rosicleire; Senhorini,José Augusto.
Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semen characteristics; Cryopreservation; Brycon cephalus; Ultrastructure; Fish.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500015

The sensorial structures of Spondylus americanus Hermann,1781 (Mollusca: Bivalvia, Spondylidae)
Provedor de dados: 52
Autores: Viana,Marina Gomes; Rocha-Barreira,Cristina de Almeida.
Adult animals of Spondylus americanus were analyzed and specialized sensorial structures, the sensorial papillae and the eyes, were characterized. The sensorial papillae were projections on the edge of the mantle in form of light brown fingers which had function of percepting stimulus coming from the medium. They were constituted by loose conjunctive tissues with longitudinal muscular fibers and externally covered by ciliated cubic epithelium, which presented high amount of melanocytes that gave the coloration of the papillae. The eyes were more complex, being on small stalks histologically similar to the papillae. They exhibited a circular formate and had a metallic blue color. Theses structures were constituted by a corneo epithelium, cornea, lens,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollusca; Scallops; Eyes; Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500009

Components of fecundity and abortion in a tropical tree, Dahlstedtia pentaphylla (Leguminosae)
Provedor de dados: 52
Autores: Teixeira,Simone de Pádua; Pereira,Rodrigo Augusto Santinelo; Ranga,Neusa Taroda.
Studies were conducted on pollination and patterns of fruit and seed production to assess the potential factors causing high fruit and seed abortion in Dahlstedtia pentaphylla (Leguminosae) through analyses of relationships between flower position in the inflorescence and ovules and seeds within ovaries and fruits, and flower, fruit and seed abortion. No differences were found in pollination and fruit set as a function of inflorescence position. There was no relationship, neither between degenerating ovules and their position within the ovary, nor between developing seed and fruit position. Maternal resource limitation related to fruits and embryos, due to the high cost of fruit production and seed maturation, was one of the factors that led to a high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abortion; Atlantic Rainforest; Dahlstedtia pentaphylla; Leguminosae; Reproduction.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700007

Seasonal variation in larval density of Limnoperna fortunei (Bivalvia, Mytilidae) in the Iguaçu and Paraná rivers, in the region of Foz do Iguaçu, Paraná, Southern Brazil
Provedor de dados: 52
Autores: Pestana,Débora; Pie,Marcio Roberto; Ostrensky,Antonio; Boeger,Walter Antonio; Andreoli,Cleverson; Franceschi,Francisco; Lagos,Patrícia.
The larval density of the invasive mussel Limnoperna fortunei (Bivalvia, Mytilidae) was monitored from January 2005 to February 2006 at two points along the Paraná and the Iguaçu Rivers, near Foz do Iguaçu, PR, Brazil. The results indicated two density peaks in the Paraná River: a lower peak between March and June and a higher peak in October. In the Iguaçu River, the values were much lower, yet they also showed a peak between September and October. A reduction in the reproductive activity was observed in both the rivers between July and August. The average larval density at the collection point in the Paraná reached 948.5 larvae/m³, with a peak of 2,999.5 larvae/m³ in October. The present study represented the first quantification of the larval density of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Invasive species; Golden mussel; Biofouling; Larval density.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300023

A simple method to estimate spatial complexity in aquatic plants
Provedor de dados: 52
Autores: Dibble,Eric David; Thomaz,Sidinei Magela.
We used a computerized approach to measure spatial complexity for the structural habitat provided by eight aquatic plant species collected from backwater lagoons located in the Upper Paraná River floodplain, Brazil. The plant species were: Cabomba furcata Schult. and Schult.f., Eichhornia azurea (Sw.) Kunth (stems and roots), Eichhornia crassipes (Mart.) Solms, Egeria najas Planchon, Heteranthera cf. zosterifolia, Potamogeton cf pusillus, Utricularia foliosa L., and Nymphaea amazonum Mart. and Zucc. The upper 0.5 m length of the terminal stems was quantified for complexity. Mean frequency and length of the interstices were significantly different among plant species. Spatial complexity varied (F = 17.30; p &lt; 0.0001) among the different plant species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic plant structure; Spatial complexity; Architecture; Habitat heterogeneity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400010

Distribution of stream macroalgal communities from the mid-western region of Paraná state, southern Brazil: importance of local scale variation
Provedor de dados: 52
Autores: Branco,Ciro Cesar Zanini; Krupek,Rogério Antônio; Peres,Cleto Kaveski.
Twenty-two stream segments, representing the diverse types of environments in the mid-western region of the Paraná State, southern Brazil, were sampled for occurrence of macroalgal communities from May to October in 2002. Twenty-seven macroalgal taxa were found, with Chlorophyta as the dominant algal group, followed by Cyanobacteria, Rhodophyta and Chrysophyta. The most widespread species was Phormidium retzii. Distribution was patchy, with species number per sampling sites ranging from zero to six and correlated positively with the abundance. On the other hand, no significant correlation was found among the species number and abundance with environmental variables. Results indicated wide and random variation among the streams. Thus, it seemed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Local-scale variation; Paraná State; Stream macroalgae; Restricted distribution; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200015

Investigation of the Acute Effects of Dry Extract of Glycine Max on Postprandial Glycemia in Rats
Provedor de dados: 52
Autores: Travassos,Patrícia Batista; Vilela,Vanessa Rodrigues; Antunes,Marina Masetto; Bazotte,Roberto Barbosa.
The acute effects of Glycine max (GM) on post prandial glycemia (PPG) in male Wistar rats were investigated. All substances were orally administered by gavage in overnight fasted animals. The elevation of PPG promoted by starch (1g/kg) was prevented by GM (2.5 mg/kg, 5.0 mg/kg, 7.5 mg/kg, 10.0 mg/kg, and 100.0 mg/kg). In conclusion GM showed potential antidiabetic effect.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Soy; Diabetes; Acarbose; Phytotherapy; Post prandial glycemia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100316

Screening and enzymatic study of a composite microbial system FH3
Provedor de dados: 52
Autores: Zhang,Yi-Min; Lu,Xue-Bin; Dan,Han-Bin; Sun,Ya-Kai.
Five strains of fungi and 18 strains of bacteria were isolated from the soil and horse manure with cellulose as the sole carbon source. Among them, two fungal, F9 and F13, and two bacterial, B16 and B21, showed the highest filter paper activities, which were 7.79 U g-1, 9.84 U g-1, 7.34 U g-1 and 9.68 U g-1, respectively. Four microbial systems, designed as FH1 (F13+B21), FH2 (F13+B16+B21), FH3 (F9+B16+B21) and FH4 (F13+F9+B16+B21) were developed. The fermentation studies showed that the filter paper activity of the composite microbial system FH3 was higher than the others, which was 21.34 U g-1. The medium with bran and filter paper as the carbon source and peptone as nitrogen source was optimal and the maximum cellulase activity was reached at 30~35ºC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulose-degrading bacterium; Composite microbial systems; Enzyme properties.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100005

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks
Provedor de dados: 52
Autores: Ashraf,Asma; Shah,Muhammad Salah-ud-Din; Habib,Mudasser; Hussain,Mujahid; Mahboob,Shahid; Al-Ghanim,Khalid.
ABSTRACT Newcastle disease (ND) is a major infectious disease of the poultry caused by a virulent strain of Avian Paramyxovirus - 1, that is a single strand non-segmented negative sense RNA virus. ND virus is major threat to the poultry industry in many countries of the world. The study was aimed to isolate and identify Newcastle disease virus (NDV) by using a haemagglutination inhibition (HI) test and reverse transcription-polymerase chain reaction (RT-PCR) assay. A total 100 samples of infected and dead birds were collected from different poultry farms. The weight of the birds was ranged 1000-1200g. The birds were divided into 3 groups. Haemagglutination assay (HA) was performed to detect the presence of NDV in suspension of infected homogenized tissues...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Newcastle disease; Epidemic; Molecular characterization; Avian virus; RT-PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100332

Drug interaction with radiopharmaceuticals: a review
Provedor de dados: 52
Autores: Bernardo-Filho,Mario; Santos-Filho,Sebastião David; Moura,Egberto Gaspar de; Maiworm,Adalgisa Ieda; Orlando,Margarida Maria de Camões; Penas,Maria Expósito; Cardoso,Valbert Nascimento; Bernardo,Luciana Camargo; Brito,Lavínia de Carvalho.
Clinical images are worthwhile in Health Sciences and their analysis and correct interpretation aid the professionals,such as physicians, physiotherapists and occupational therapists, to make decisions and take subsequent therapeutic and/or rehabilitation measures. Other factors, besides the state of the disease, may interfere and affect the bioavailability of the radiopharmaceuticals (radiobiocomplexes) and the quality of the SPECT and PET images. Furthermore, the labeling of some of these radiobiocomplexes, such as plasma proteins, white blood cells and red blood cells, with 99mT, can also be modified. These factors include drugs (synthetic and natural) and dietary conditions, as well as some medical procedures (invasive or non-invasive), such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug interaction; Blood constituents; Radiobiocomplexes; Bioavailability.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700003

Effect of Salicylic Acid, 2,4-D and 2i-P on the Production of Secondary Metabolites in Garcinia brasiliensis Mart. Callus
Provedor de dados: 52
Autores: Teixeira,Maria Gessi; Carvalho,Marília; Leite,Marco Aurélio; Barbosa,Sandro; Santos filho,Plinio Rodrigues dos; Santos,Breno Régis.
Abstract The aim of this work was to evaluate the effect of Salicylic acid, 2,4-D and 2-iP on the production of total phenolic and flavonoids as well in the levels of fukugetin and 7-epiclusianone in callus of Garcinia brasiliensis zygotic embryos. For this, Bacupari callus were exposed to different concentrations (0.0; 0.1; 1; 10; 100 µM) of Salicylic acid (SA) in the presence or in the absence of 72 μM 2-iP and 28.73 μM 2,4-D. The highest concentration of total phenolic occurred in the treatment with 100 μM SA in callus subcultured in the absence of 2,4-D and 2-iP, and with 10 μM SA in callus subcultured in the presence of these regulators. Concerning flavonoids, 2,4-D and 2-iP supplementation without the presence of the SA was sufficient for the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacupari; Phenols; Flavonoids; 7-epiclusianone; Fukugetin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100407

In Vitro Mid-Term Conservation of Acorus calamus L. via Cold Storage of Encapsulated Microrhizome
Provedor de dados: 52
Autores: Quraishi,Afaque; Mehar,Snigdha; Sahu,Durga; Jadhav,Shailesh Kumar.
ABSTRACT In vitro rhizome production, encapsulation and cold storage of Acorus calamus were attempted for its propagation and ‘true-to-type’ conservation. Shoot cultures were initiated using underground rhizome buds, on 6-benzyladenine (BA) containing Murashige and Skoog (MS) medium. Maximum microrhizome production was observed in presence of 33.3 µM BA, on modified MS medium containing 6% sucrose, 100 mg/L citric acid and 1 g/L polyvinyl pyrrolidone-40. Synthetic seeds were produced from regenerated microtubers by encapsulation in calcium alginate beads. These synthetic seeds were stored in complete darkness at 100C temperature for different durations for mid-term conservation. After cold storage, synthetic seeds were re-cultured in vitro, 100% survival...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aromatic plant; Encapsulation; Sweet Flag; Synthetic seeds; Plant tissue culture.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100412

Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]
Provedor de dados: 52
Autores: Corrêa,Cynthia Manyra; Oliveira,Graciele Nicolodi de; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plant; Tissue culture; Polymnia sonchifolia; Micropropagation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300005

Microbiological analysis of critical points in the chicken industry
Provedor de dados: 52
Autores: Cansian,Rogério Luis; Floriani,Salete Teresa Radeski; Valduga,Eunice.
This work is focused on identifying microbial contamination in the scalding asepsis and cooling processes as well as in fresh sausages obtained. Salmonella was identified in two scald water samples but was absent in the water from chiller and in the final product, which might be explained in terms of chlorine addition and temperature reduction. The analysis revealed that MPN of Escherichia coli was in the range of &lt;1-11/mL in the scald water and &lt;1-64/mL in the chiller. Aeromonas sp. count showed 5 to 3.5x10¹ CFU/mL in the scald water and 9 to 3.7x10² CFU/mL in the chiller, probably due to the psycrophile characteristics of Aeromonas and evisceration process. The analysis of fresh sausages chicken revealed a sharp increase in the Aeromonas count...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas; Bacteria; Chicken; Microbiology; Food quality control.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300011

Hydrolysis of galacto-oligosaccharides in soy molasses by α -galactosidases and invertase from Aspergillus terreus
Provedor de dados: 52
Autores: Reis,Angélica Pataro; Guimarães,Valéria Monteze; Ferreira,Joana Gasperazzo; Queiroz,José Humberto de; Oliveira,Maria Goreti Almeida; Falkoski,Daniel Luciano; Almeida,Maíra Nicolau de; Rezende,Sebastião Tavares de.
Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ÂºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ÂºC, respectively. The K M app for Ï -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ÂºC and invertase maintained about 90% after 6 h at 55 ÂºC. P1 and P2 presented different inhibition sensitivities by Ag+,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus terreus; Galactooligosaccharides; Α-galactosidase; Invertase; Soy molasses.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300029

Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides
Provedor de dados: 52
Autores: Rodrigues,José Ariévilo Gurgel; Vanderlei,Edfranck de Sousa Oliveira; Bessa,Érika Façanha; Magalhães,Francisco de Araújo; Paula,Regina Célia Monteiro de; Lima,Vilma; Benevides,Norma Maria Barros.
The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine algae; Sulfated macromolecules; Chemical analysis; APTT test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007

Lipase Production by Aspergillus niger C by Submerged Fermentation
Provedor de dados: 52
Autores: Lima,Laisy Garcia Ribeiro; Gonçalves,Márcia Monteiro Machado; Couri,Sonia; Melo,Verônica Ferreira; Sant’Ana,Gizele Cardoso Fontes; Costa,Antônio Carlos Augusto da.
Abstract This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 25-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 22 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 ammonium sulphate, 4.0 g.L-1 soybean oil and 1.0 g.L-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipases; Submerged fermentation; Aspergillus nige; Experimental design.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100214

Atraumatic restorative treatment - glass ionomer sealants survival after a postgraduate training program in Ecuador: 2-year follow-up
Provedor de dados: 52
Autores: Rios,Edisson Lopez; Diniz,Ivana Márcia Alves; Ruiz,Oswaldo; Marques,Márcia Martins.
This study aimed to evaluate the survival of atraumatic restorative treatment high-viscosity glass ionomer sealants (ART-hvGIS) and its relationship with carious lesions incidence in underserved communities of Ecuador. A total of 483 first permanent molars with ART-hvGIS were included after treatment, in which fifteen students of the First Preventive Dentistry Post Graduate Program of Universidad Central del Ecuador assisted 176 schoolchildren, from 5 to 12 year-old. All the students had previously participated in a training course in ART approach. After one and two years follow-up, schoolchildren were reevaluated in relation to sealants retention and new carious lesions development. After first year of evaluation ART-hvGIS retention rate was about 30% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental atraumatic restorative treatment; Glass ionomer cements; Pit and fissure sealants; Latin America.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100049

Antioxidant Status, Lipid Peroxidation and Testis-histoarchitecture Induced by Lead Nitrate and Mercury Chloride in Male Rats
Provedor de dados: 52
Autores: Bas,Hatice; Kalender,Suna.
ABSTRACT This study was done to evaluate the effects of lead nitrate and mercury chloride in testis tissues of Wistar rats. Lead nitrate and mercury chloride are widely used heavy metals in industry. Oral lead and mercury administrations to adult male rats at doses 45 mg/kg bw and 0.02 mg/kg bw, respectively for 4 weeks caused a significant increasing in MDA levels and antioxidant enzyme activities (SOD, CAT, GPx and GST). The MDA levels and acivities of antioxidant enzymes was lower in rats that were administrated by lead nitrate than mercury chloride treated group. Light microscopic analyses revealed that lead nitrate and mercury chloride induced numerous histopathological changes in testis tissues of rats. Histopathological observations of the testis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Lead; Mercury; Oxidative stress; Testis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100341

The influence of freezing rates on bovine pericardium tissue Freeze-drying
Provedor de dados: 52
Autores: Borgognoni,Camila Figueiredo; Tattini Junior,Virgilio; Ayrosa,Ana Maria Irene Bartolomeu; Polakiewicz,Bronislaw; Leirner,Adolfo Alberto; Maizato,Marina Junko Shiotsu; Higa,Olga Zazuco; Beppu,Marisa Masumi; Pitombo,Ronaldo Nogueira de Moraes.
The bovine pericardium has been used as biomaterial in developing bioprostheses. Freeze-drying is a drying process that could be used for heart valve's preservation. The maintenance of the characteristics of the biomaterial is important for a good heart valve performance. This paper describes the initial step in the development of a bovine pericardium tissue freeze-drying to be used in heart valves. Freeze-drying involves three steps: freezing, primary drying and secondary drying. The freezing step influences the ice crystal size and, consequently, the primary and secondary drying stages. The aim of this work was to investigate the influence of freezing rates on the bovine pericardium tissue freeze-drying parameters. The glass transition temperature and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freeze-drying; Freezing; Bovine pericardium.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600021

Spatial variation of five co-existing siluriformes in an atlantic rain forest drainage
Provedor de dados: 52
Autores: Mazzoni,Rosana; Lemos,Fernanda de Souza; Iglesias-Rios,Ricardo.
Five species of Siluriformes were registered in the Ubatiba system. Pimelodella lateristriga was the dominant one, followed by Hypostomus gr. punctatus, Rineloricaria sp., Callichthys callichthys and Rhamdia sp. Simple correlation analysis between species density and habitat parameters indicated that hydrology explained density patterns of four species. Pimelodella densities were negatively correlated with pools, Callichthys and Hypostomus densities were positively correlated with runs and Rhamdia densities were positively correlated with riffles; Rineloricaria densities did not respond to any hydrological parameter. Substrata were an important factor to all species, but specific preferences were observed. Marginal vegetation was positively correlated only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal stream; Habitat characteristics; Distribution; Catfishes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100017

Fish assemblages in a tidal flat
Provedor de dados: 52
Autores: Vendel,Ana Lúcia; Lopes,Sabine Granado; Santos,César; Spach,Henry Louis.
Studies were carried out on fish assemblages in a tidal flat. Samples were obtained monthly at low tide of the half moon in the tidal flat of Paranaguá Bay, Brazil, with two seine nets, one with a 1 mm mesh, 30 m in length and 3 m in height and another with a 10 mm mesh, 65 m in length and 2 m in height. A total of 8,890 fish were captured, comprising 24 families and 53 species. The most abundant species were Harengula clupeola and Atherinella brasiliensis, which represented 63.4% of the total, capture. A seasonal tendency was observed in the abundance of fishes, with less fishes being captured during winter and part of spring. The number of species showed a seasonal pattern, with the gradual decrease through winter and a marked increase in summer. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tidal flat; Fish assemblages; Seasonal variation; Brazil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200015

Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation
Provedor de dados: 52
Autores: Gonçalves,Aline Zorzetto Lopes; Carvalho,Ana Flávia Azevedo; Silva,Roberto da; Gomes,Eleni.
Thermophilic Thermomyces lanuginosus strain TO3 was isolated from compost pile samples and was used for its ability to produce considerable glucoamylase activity when growing in liquid medium at 45ºC with starch as the sole carbon source. Enzyme productivity was high in submerged fermentation (SmF) with maximum activity of 13 U/mL after 168 h of fermentation. Higher quantities of glucose were released when the substrate for enzyme was soluble starch than maltose or maltooligosaccharides were used. The distribution of glucoamylase between the extracellular and cell-associated fractions varied according to fermentation time. Glucoamylase produced from T. lanuginosus TO3 had optimum activity at 65 ºC and good thermostability in the absence of substrate, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucoamylase; Submerged fermentation; Thermomyces lanuginosus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400024

The effect of salinization in maintenance of intermittent catheter
Provedor de dados: 52
Autores: Gil,Nelly Lopes de Moraes; Santos,Izolete Aparecida Tomazine; Carvalho,Maria Dalva de Barros.
The aim of this study was to carry out a comparative analysis between the intermittent catheter, filled with heparin 100 U/ml and one filled with saline solution 0.9%., by evaluating the formation of clots and quantifying them, when detected. Four groups of seven rabbits were used. In two of the groups, saline solution was used; in one group, in case of resistance, it was forced, whereas in the other, it was not forced. The other two groups were subjected to the same procedure, but, heparin solution was used. The presence of clot formation in all the groups was observed, independently of the solution used.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intermittent catheter; Heparin solution; Saline solution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100009

Evaluation of the microbial diversity in sequencing batch reactor treating linear alkylbenzene sulfonate under denitrifying and mesophilic conditions using swine sludge as inoculum
Provedor de dados: 52
Autores: Duarte,Iolanda Cristina Silveira; Oliveira,Lorena Lima de; Okada,Dagoberto Yukio; Prado,Pierre Ferreira do; Varesche,Maria Bernadete Amâncio.
The objective of this study was to evaluate the degradation of Linear Alkylbenzene Sulfonate (LAS) in anaerobic sequencing batch reactor (ASBR) under denitrifying conditions using swine sludge as inoculum. The reactor was operated for 104 days with synthetic substrate containing nitrate, and LAS was added later (22 mg/L). Considering the added mass of the LAS, the adsorbed mass in the sludge and discarded along with the effluent, degradation of the surfactant at the end of operation was 87%, removal of chemical oxygen demand was 86% and nitrate was 98%. The bacterial community was evaluated by cutting the bands and sequencing of polymerase chain reaction (PCR) fragments and denaturing gradient gel electrophoresis (DGGE). The sequences obtained were related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linear alkylbenzene sulfonate (LAS); Bioreactor; PCR/DGGE; Denitrified.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300326

Morphology and ecology of bivalve molluscs from Admiralty Bay, King George Island, Antarctica
Provedor de dados: 52
Autores: Absher,Theresinha M.; Feijó,Angela R.
Bivalve species were collected from shallow coastal areas of King George Island (Martel, Mackellar and Ezcurra Inlets of Admiralty Bay). Twenty one species belonging to 16 genera and 12 families were identified and their morphometric and morphological shell characteristics were described. Three main characteristics were found to be common to the majority of the bivalve species sampled: 1) thin fragile shells; 2) small size of individuals (76%), and 3) the lack of true cardinal teeth (72%). Comparison of calcium data from a tropical estuary and a subantarctic coastal shallow area suggested that the calcium in the sea water was not a constraint to shell building but shell thickness could be an adaptation to the efficiency of energy partitioning. Small...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bivalve species; Antarctic bivalves; King George Island; Antarctica.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400008

Total mercury in the night shark, Carcharhinus signatus in the western equatorial Atlantic Ocean
Provedor de dados: 52
Autores: Ferreira,Alexandre Gomes; Faria,Vicente Vieira; Carvalho,Carlos Euardo Veiga de; Lessa,Rosangela Paula Teixeira; Silva,Francisco Marcante Santana da.
Mercury is the only element capable to biomagnificate along the food chain, and carnivorous fish tend to accumulate high concentrations of this element. Total mercury in muscle tissue of Carcharhinus signatus sampled along the western equatorial Atlantic Ocean was measured by atomic emission spectrophotometry with ICP/AES. The average total mercury concentrations were consistently greater than the maximum limit for human consumption established by the Brazilian Health Ministry for carnivorous fishes (1000 µg. kg-1 w.w.). Therefore, an average consumption of 0.1 kg/day of C. signatus would result in an average daily ingestion of 174.2 µg of Hg, more than five times the 30 µg/day intake established by the World Health Organization. In the range of lengths...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Shark; Carcharhinus signatus; Southwestern Atlantic Ocean.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400016

The influence of water renewal rates on the reproductive and molting cycles of Penaeus paulensis in captivity
Provedor de dados: 52
Autores: Peixoto,Sílvio; Cavalli,Ronaldo Olivera; Wasielesky Junior,Wilson.
The present study analyzed the reproduction of wild-caught Penaeus paulensis in relation to its molt cycle. The experimental design consisted of two treatments (continuous water flow and batch renewal) with two replicates. The stocking density in experimental tanks (1.50 x 0.96m) was approximately 7 animals/m², resulting in 4 males (23.9 ± 2.3 g) and 6 females (52.0 ± 5.5 g) per tank. Shrimp had their uropods cut for individual marking and female maturation was induced through the unilateral eyestalk-ablation. The intermolt period of females and males (17.4 ± 3.2 and 17.8 ± 4.6 days, respectively), number of days between molting and the first spawn (6.9 ± 2.8 days) and number of spawns in the intermolt period (1.4 ± 0.5), presented no significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molt; Maturation; Penaeid.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200020

In vitro multiplication of 'Flordaguard' rootstock: cytokinin source and concentration effects, explants orientation and period of permanence in the culture medium
Provedor de dados: 52
Autores: Radmann,Elizete Beatriz; Bianchi,Valmor João; Fachinello,José Carlos; Ferreira,Letícia Vanni; Oliveira,Roberto Pedroso de.
This work aimed at studying the effects of different sources and cytokinin concentrations, as well as explants orientation and time of their permanence in shoots induction medium to obtain high multiplication rate. Four experiments were carried out to evaluate the sprout percentage mean, shoots number per explant, elongated shoots percentage and shoots length. The treatments with 2iP and zeatin did not promote in vitro plant multiplication. When different BAP concentrations were tested, an increase in sprout percentage was obtained using BAP concentration up to 3.66 mg L-1 (80%), and for shoots number per explant a linear behavior was observed, showing a mean of four shoots per explant with 4.0 mg L-1 BAP. For the 'Flordaguard' rootstocks, the explants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micropropagation; Cytokinin; Explant; Prunus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100004

Production of yellow-green fluorescent pigment by Pseudomonas fluorescens
Provedor de dados: 52
Autores: Silva,Gildo Almeida da; Almeida,Erik Amazonas de.
A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas fluorescens; Siderophore; Yeast extract; Yellow-green fluorescent pigment.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400009

Determination of the potential of Pistia stratiotes L. in removing nickel from the environment by utilizing its rhizofiltration capacity
Provedor de dados: 52
Autores: LEBLEBİCİ,Zeliha; DALMIŞ,Esra; ANDEDEN,Enver Ersoy.
Abstract: The aim of this study is to explain the biological response and rhizofiltration capacity of Pistia stratiotes, which is an aquatic macrophyte, in alleviating heavy metal stress. In our study, Pistia species were exposed to Nickel (Ni) at different concentrations over seven days. The potential of the aquatic macrophytes in accumulating heavy metals in the water and in removing them from the environment was evaluated by determining the bio-concentration factors (BCF). Selected biological parameters in the leaves of Pistia stratiotes, including metal accumulation, photosynthetic pigment amount, lipid peroxidation activity, and growth rates were measured at the end of the seven-day period. The highest amount of Ni accumulation in P. stratiotes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metal; Bioconcentration factor; Pistia stratiotes; Rhizofiltration.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100703

Biodegradation of stored jet fuel by a Nocardia sp. isolated from contaminated soil
Provedor de dados: 52
Autores: Gomes,Edelvio de Barros; Soriano,Adriana Ururahy; Miranda,Rita de Cássia Mendonça de; Sousa,Maria de Fátima Vieira de Queiroz; Pereira Jr.,Nei.
The aim of this study was to investigate the potential of degradation of an autochthonous bacterial strain, isolated from petroleum derivatives contaminated soil samples against jet fuel hydrocarbons. The autochthonous bacterial strain was characterized as Nocardia sp. Evaluation of their degrading abilities was carried out by presumptive assays as redox indicator test and by observations of surface tension decreases in aqueous medium. Degradation of jet fuel hydrocarbons was evaluated by chromatographic methods. Experiments were performed in flasks at two biostimulation rates. A bacterial strain of Pseudomonas aeruginosa UFPEDA 39 was utilized as a reference microorganism. The bacterial strain, identified as Nocardia sp, demonstrate high ability to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Nocardia; Jet fuel.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500027

Physicochemical stability of natural or pre-sweetened frozen passion fruit juice
Provedor de dados: 52
Autores: Righetto,Andrea Mara; Beleia,Adelaide; Ferreira,Sandra Helena P.
Passion fruit juice, pure and sucrose sweetened (1:1, w:v), was frozen and stored for 8 months in freezers. The effect of storage time and sucrose addition on physicochemical properties of the juices was evaluated, in the fresh juice, and then every two months in the stored samples. Concentration of soluble solids, organic acids, total and reducing sugars and ascorbic acid, as well as pH were not affected by storage time. Initially sucrose addition modified the juice color, but not during storage time, while the natural juice became more yellow during storage. β -carotene content decreased as a function of storage time, 51.3 % in the pure juice and 29.7 % in the sweetened juice.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passion fruit; Frozen storage; Fruit juices.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400003

Harmful phytoplankton blooms and fish mortality in a eutrophicated reservoir of northeast Brazil
Provedor de dados: 52
Autores: Chellappa,Naithirithi Tiruvenkatachary; Chellappa,Sarah Laxhmi; Chellappa,Sathyabama.
The aim of this work was to study the eutrophication in the tropical freshwater ecosystems and the consequent cyanobacterial bloom formation and economical damage to fisheries and harmful effects to public health. Mass fish mortality due to toxin producing cyanobacterial blooms was registered during December 2003 in Marechal Dutra Reservoir, Acari/RN, Northeast Brazil. Phytoplankton and fish samplings were carried out on alternate days during the episode of fish mortality and monthly during January to June 2004. The cyanobacterial toxin was identified and quantified from the seston samples and liver of the dead fishes using the standard HPLC method. The results indicated that the toxic blooms of Cylindrospermopsis raciborskii and Microcystis aeruginosa...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Harmful algal blooms; Anthropogenic activity; Fish mortality; Reservoir; Public health.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400022

Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
Provedor de dados: 52
Autores: Papamichael,Emmanuel M; Roustas,Michael K; Bieth,Joseph G.
In this study, the S1 - P1 and S3 - P3 interactions between papain and four synthetic peptide substrates were found as to be important. The values of Km were estimated as to be practically identical between these substrates; this latter is supporting the conclusions obtained by considering the estimated values of other kinetic parameters. Nevertheless, based on the estimated kcat and/or k cat/Km parameters of the used substrates, we concluded that an aromatic ring at the P3 position, and a positively charged side chain of the residue at the P1 position of the synthetic substrates were favored considerably their interaction with papain.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Papain-Substrates; Enzyme-Substrate Interactions.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300002

Xylitol inhibits J774A.1 macrophage adhesion in vitro
Provedor de dados: 52
Autores: Ferreira,Aline Siqueira; Souza,Maria Aparecida de; Raposo,Nádia Rezende Barbosa; Ferreira,Ana Paula; Silva,Silvio Silvério da.
The aim of this work was to evaluate the effect of xylitol on J774A.1 macrophage adhesion. Adhesion consisted of a three-hour interval, at room temperature, followed by washing and cell incubation at 37ºC/5% CO2/ 48h. Xylitol was used to treat the cells either before (for 24h) or after the cell incubation (for 48h) at 5% as final concentration in both the situations. It was found that xylitol was effective in preventing the adhesion in both the conditions in spite of the former being 100-fold greater and significant (p &lt; 0.001). The results pointed to an important xylitol action on macrophage adhesion, which should be further investigated as an inflammatory control.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; J774A; 1 macrophages; Xylitol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600017

Rheumatoid factor and tobacco exposure
Provedor de dados: 52
Autores: Skare,Thelma Larocca; Silva,Marilia Barreto Gameiro; Silva,Mônica Gomes da; Gomes,Fábio; Rumor,Carla; Giacomini,Glaucio.
This study was carried out to analyse the possible influence of smoking on the positivity of rheumatoid factor (RF) in healthy individuals in the region of Curitiba, Brazil. One hundred fifty blood donors (84 smokers and 66 non-smokers ) were studied for the presence of RF using nephelometry. Smoking was found not to be correlated to the positivity of RF, independently of the smokers sex (men with p=0.09 and women with p=0.49), the number of cigarettes/day (p=0.20), duration of smoking (p=0.18) and cumulative exposure (time X quantity) to cigarettes (p=0.13).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid factor; Smoking; Rheumatoid arthritis; Tobacco exposure.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200012

Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
Provedor de dados: 52
Autores: Mariano,André Bellin; Kovalhuk,Leonardo; Valente,Caroline; Maurer-Menestrina,Juliana; Pereira-Netto,Adaucto Bellarmino; Guerra,Miguel Pedro; Carnieri,Eva Gunilla Skare.
A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria angustifolia; Callus; Plant mitochondria; Respiratory chain.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006

Investigation of Some Parameters Affecting Methyl OrangeRemoval by Fusarium acuminatum
Provedor de dados: 52
Autores: Yucel,Tugba Tugrul.
ABSTRACT Dye stuff released to the ecosystem from textile industries cause a serious contamination and become a major environmental problem over the last few decades. As biological decolorization of textile wastewater is an important issue, Fusarium . acuminatum was used to removal of a frequently used textile dye, methyl orange. Live pellet of Fusarium acuminatum was used and decolorization studies performed in various temperatures and pH conditions with different dye concentrations. The highest decolorization rate was observed at 35ᴼC. 60 mg/L was found as the optimum initial dye concentration. In the pH range of 3-4, decolorization rate was approximately 70%. It was seen that Fusarium acuminatum have the great ability of the methyl orange removal. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosorption; Azo dye; Methyl orange; Fusarium acuminatum; Live pellet.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100419

Assessment of Rice Associated Bacterial Ability to Enhance Rice Seed Germination and Rice Growth Promotion
Provedor de dados: 52
Autores: Gholamalizadeh,R.; Khodakaramian,G.; Ebadi,A. A.
ABSTRACT The application of beneficial bacteria has recently been used for sustainable agriculture. In current research, 71 bacterial isolates were obtained from rice plant and the rhizosphere soil of different paddy fields in Guilan province, Iran. After primitive investigation, 40 bacteria with typical predominant characteristics were selected. By PCR-RFLP of their 16S r-DNA gene, 8 Operational Taxonomic Units (OTUs) totally consisted of 33 isolates were obtained. From all of them, 8 isolates were selected for rice seed germination experiment, then, effective isolates were used for pot experiment to evaluate their ability for promoting rice growth. All of them were able to increase rice growth and yield, but in different potential. These tested isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice associated bacteria; Plant growth promoting bacteria; PCR-RFLP of 16S r-DNA; IAA production; Phosphate solubilizing bacteria.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100204

Chemical Composition and Biological Activities of The Essential Oil And Anatomical Markers Of Lavandula Dentata L. Cultivated In Brazil
Provedor de dados: 52
Autores: Justus,Barbara; Almeida,Valter Paes de; Gonçalves,Melissa Marques; Assunção,Daniele Priscila da Silva Fardin de; Borsato,Debora Maria; Arana,Andres Fernando Montenegro; Maia,Beatriz Helena Lameiro Noronha Sales; Paula,Josiane de Fátima Padilha de; Budel,Jane Manfron; Farago,Paulo Vitor.
ABSTRACT Lavandula dentata, popularly known as lavender, is commonly used in traditional medicine for the treatment of digestive and inflammatory disorders. The objective of this study was to analyzed the chemical oil composition, antioxidant and antimicrobial activities of the essential oil and anatomical markers of the leaf and stem of L. dentata cultivated in South Brazil. Essential oil showed an antioxidant activity similar to rutin and gallic acid when analyzed by phosphomolybdenum method. However, by the free radical DPPH and ABTS methods, it showed a slight potential antioxidant. Essential oil presented 1,8-cineol (63%) as major component, antimicrobial activity against Gram-positive, Gram-negative bacteria strains and Candida albicans, by broth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Antimicrobial; Antioxidant; Lamiaceae; Quality control; Volatile oil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100441

Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants
Provedor de dados: 52
Autores: Ulisses,Cláudia; Camara,Terezinha Rangel; Willadino,Lilia; Albuquerque,Cynthia Cavalcanti de; Brito,Júlio Zoé de.
The present work evaluated the development of embryogenic callus from transversal ovary sections. The experiments were carried out under two experimental regimes using combinations of IAA (0; 5.71; 8.56; 11.42; 14.27μM) and 2,4-D (0; 13.57; 18.10; 22.62μM) or combinations of 2,4-D with BA (0; 4.43; 6.65; 8.87; 11.09μM). Assessments were made of anatomical aspects of the callus and for the presence of embryogenic structures using cytochemical and histological analyses and stereomicroscopic and scanning electronic microscopic observations. Treatments with 2,4-D and IAA produced friable calluses demonstrating cellular acquisition of morphogenetic competence as well as the formation of pro-embryogenic sectors. The expression of embryogenic program could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callogenesis; Growth regulator; Histology; Scanning electron microscopy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100002

Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae
Provedor de dados: 52
Autores: Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain.
In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175

Gas Chromatography - Mass Spectrometry Analysis and Antibacterial Activity of Bluish-Green Pigment from Pseudomonas sp. JJTBVK (KF836502)
Provedor de dados: 52
Autores: Verma,Bala; Kumar,Pradeep; karthik,Loganathan; Dhanasekaran,Dharumadurai; Babalola,Olubukola Oluranti; Banakar,Shivakumar Pulakeshappa.
The present study was conducted for the isolation of potential bacteria from the desert soil, their molecular identification and prediction of restriction sites of the potential isolate using the bioinformatics tools. Production of the metabolites was done by inoculating in nutrient broth of pH 8.6. Metabolite was bluish-green in color; it was extracted and dried by using methanol and used for partial characterization by using GC-MS spectroscopy. Antibacterial activity was performed with the clinical human pathogenic isolates. The bacterium was identified as Pseudomonas sp.JJTBVK on the basis of 16S rRNA sequencing. The sequence was analyzed for the restriction cleavage sites, which showed that the sequence had various restriction sites for different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterisation; Pseudomonas; Extracellular; Pigment; Antibacterial activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400628

Comparisons Between Different Methods in Measuring Enzyme Similarity for Metabolic Network Alignment
Provedor de dados: 52
Autores: Zhou,Wenwei; Liu,Jiangtao; Xing,Hiu; Zhang,Zhengdong; Xie,Xiaoyao; Jing,Fengxuan.
Metabolic network alignments enable comparison of the similarities and differences between pathways in two metabolic networks and help to uncover the conserved sub-blocks therein. Such analysis is important in the understanding of metabolic networks and species evolution. The fundamental parts of metabolic network alignment algorithms all involve comparisons of the similarity between two enzymes as a similarity measure of network nodes. As a result, the study of methods for measuring enzyme similarity becomes highly relevant. Currently, two approaches are mainly used to measure enzyme similarity. One of the methods is based on similarity measures of gene or protein sequences; the other is based on enzyme classification. In this study, multiple metabolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic network alignment; Enzyme; Similarity measure; Classification numbering; Sequence similarity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100405

Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
Provedor de dados: 52
Autores: Rahulan,Raji; Manjunath,G; Nampoothiri,Kesavan Madhavan; Pandey,Ashok.
A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomyces gedanensis; Methionine amino peptidase; Fermentation; Box Behnken design.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005

Optimization of biomass production with copper bioaccumulation by yeasts in submerged fermentation
Provedor de dados: 52
Autores: Arakaki,Andréa Haruko; Souza Vandenberghe,Luciana Porto de; Soccol,Vanete Thomaz; Masaki,Ryu; Rosa Filho,Ernani Francisco da; Gregório,Alexsandro; Soccol,Carlos Ricardo.
The objective of this work was to study the production of biomass with copper bioaccumulation in submerged fermentation using sugarcane molasses. Candida pelliculosa BARU 05 isolated from Baru (Dipteryx alata) was selected for its good capacity to accumulate the copper. Fermentation was carried out using the medium composed by sugarcane molasses at 5 °Brix enriched with (g/L) CuSO4.5H2O 0.1; yeast extract, 10.0; (NH4)2SO4, 5.0 ; KH2PO4, 5.0 MgSO4, 0.5, inoculum 10 % of total volume (100 ml), pH 6.0, and incubation at 30 °C, 120 rpm for 120 h. After three steps of optimization an uptake of 95.04% and 13.397 g/L biomass were obtained. The kinetics of copper bioaccumulation and biomass production was followed in a 10- liter bioreactor in a batch and fed-batch...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioaccumulation; Baru; Yeast; Copper; Sugarcane molasses.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500021

Aspectos relacionados à precocidade sexual em bovinos machos da raça Nelore, PO
Provedor de dados: 52
Autores: Silva,Antonio E. D. Feliciano; Unanian,Maria M.; Silva,Alzira Roza e.
O estudo foi desenvolvido em 79 bovinos machos da raça Nelore, PO, de dez a 16 meses de idade. Em todos os animais foram medidas a circunferência escrotal (CE), a concentração de testosterona (Testo) e o aparecimento dos primeiros espermatozoides no ejaculado. Os resultados indicaram que 18,5 % dos machos em estudo apresentaram os primeiros espermatozoides aos 306,17± 2,42 dias de idade, 19,75± 0,9 cm de circunferência escrotal, e uma concentração de testosterona de 1,57± 0,9 ng/ml. Após o aparecimento dos primeiros espermatozoides no ejaculado, os machos mostraram a puberdade aos 365,39± 1,13 dias e a maturidade aos 487,38± 3,08 dias. Estes resultados indicam que existem machos na raça Nelore, PO que iniciam a atividade sexual em idades inferiores às...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age of first spermatozoa; Scrotal circumference; Puberty; Bovine; Nelore breed.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400016

Studies of the floral biology and reproductive system of Brassica napus L.(Cruciferae)
Provedor de dados: 52
Autores: Mussury,Rosilda Mara; Fernandes,Wedson Desidério.
Studies of the floral biology of canola were accomplished with the objective of enlarging the system and reproductive strategies knowledge of its CTC-4 cultivar in Dourados, Centerwest, Brazil. Brassica napus is self pollinated, presenting however, many resources for the allogamy accomplishment, e.g., abundant pollen, nectar and scent production. The smaller anther were responsible for the production of fruit with smaller and less growth seeds, when compared to the autogamy, autopollination and cross pollination tests.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica napus; Canola; Floral biology.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100014

Production of specific polyclonal antibodies anti-human factor IX
Provedor de dados: 52
Autores: Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos.
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010

Isolation of endophytic actinomycetes from roots and leaves of maize (Zea mays L.)
Provedor de dados: 52
Autores: Araújo,Janete M. de; Silva,Adilson C. da; Azevedo,João L.
Actinomycetes were isolated from surface sterilized leaves and roots of maize. A total of 53 isolates were obtained, 31 of them from leaves and 22 from roots. The genus Microbispora was the most frequently found followed by the genera Streptomyces and Streptosporangium. From the isolated actinomycetes, 43.4% showed antimicrobial activity against one or more tested bacteria and yeast.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytes; Maize; Actinomycetes; Microbispora; Streptomyces; Streptosporangium.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400016

Influence of the use of selected and non-selected yeasts in red wine production
Provedor de dados: 52
Autores: Dorneles,Daniela; Machado,Iara Maria Pereira; Chociai,Miriam Blumel; Bonfim,Tania Maria Bordin.
The aim of this work was to study the influence of use of Saccharomyces cerevisiae selected varieties in the elaboration of Terci red wine from Colombo. The winemaking method followed the classic red wine vinification system and the samples were analyzed according to the official table wine methods. The assays performed showed differences mainly over volatile acids, acetaldehyde, esters and methanol contents, confirming that the use of selected yeasts contributed on improving the wine quality.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fermentation; Red wine; Selected yeasts.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600010

Proximate composition and fatty acid content of the mangrove oyster Crassostrea rhizophorae along the year seasons
Provedor de dados: 52
Autores: Martino,Ricardo Cavalcanti; Cruz,Gracinda Maria da.
Mangrove oysters, Crassostrea rhizophorae were collected at the mangrove of "Barra de Guaratiba" district, Rio de Janeiro, Brazil, with the aim to determine the proximate composition and fatty acid content. Along the year seasons no statistical (P&gt;0.05) difference was observed in the values of moisture, crude protein, crude lipid and ash. They were 82.0%; 9.7%; 1.7%; 3.2%, in average, respectively. However, glycogen was significantly (P&lt;0.05) higher in spring (4.4%) and winter (4.2%) samples, than in summer (2.7%) and autumn (2.9%), samples. Saturated fatty acids and polyunsaturated fatty acids were respectively, the most important fatty acids in oysters, with the palmitic acid (16:0), being the major fatty acid. This study, demonstrated that this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangrove oysters; Crassostrea rhizophorae; Proximate composition; Fatty acids; Year seasons; Human health.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600015

Use of cassava wastewater treated anaerobically with alkaline agents as fertilizer for maize (Zea mays L.)
Provedor de dados: 52
Autores: Ribas,Maria Magdalena Ferreira; Cereda,Marney Pascoli; Villas Bôas,Roberto Lyra.
The wastewater of the processing of cassava's flour (manipueira) was submitted to the anaerobic treatment in two phases: acidogenic and methanogenic. In the acidogenic phase, the wastewater was stabilized with NaOH (ASH) and with limestone (ASL). After that, both stabilized effluents were treated by a methanogenic reactor. Then, the effluent of the methanogenic reactor was used as fertilizer on maize in the initial growth stage (30 days), cultivated in pots in a greenhouse. The treatments were T1: control without urea addition (only N from soil); T2: NPK (2.2 g of urea with 45% of N); T3: ASH (84 mL.kg soil-1); T4: ASL (102 mL.kg soil-1); T5: double dose ASH (168 mL.kg soil-1) and T6: double dose ASL (204 mL.kg soil-1). Each treatment was composed by 4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reuse; Manipueira; Anaerobic digestion; Hydroxide sodium; Dolomitic limestone.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100007

Erratum: Developmental Toxicity of (4S)-2- (4-hydroxy-3-methoxyphenyl) thiazolidine- 4-carboxylic acid in Zebrafish (Danio rerio)
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100907

Physico-chemical, morphological and pasting properties of starches extracted from water Chestnuts (Trapa natans) from three Lakes of Kashmir, India
Provedor de dados: 52
Autores: Gani,Adil; Haq,Sham Sul; Masoodi,F.A.; Broadway,A. A.; Gani,Asir.
Studies on physicochemical, morphology and pasting properties of starches extracted from water chestnuts of three Lakes of Kashmir valley (Wular, Anchar and Dal Lakes) were conducted to determine their application in different food products. The water chestnut starch from Dal Lake had more oval shaped granules than water chestnut starches from the Wular and the Anchar Lakes.The unique feature of the water chestnut starches were shape of starch granules which looked like horn(s) protruding from the surface which did not appear in other starches already studied. Proximate analysis of water chestnut starches showed that average protein content were 0.4%, amylose 29.5 % and ash 0.007 on dry weight basis. Increase in water binding capacity, swelling power and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water chestnut; Morphology; Physioco-chemical properties; Pasting properties; Gel Texture.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300030

Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
Provedor de dados: 52
Autores: Carvalho,Milene Alves de Figueiredo; Paiva,Renato; Stein,Vanessa Cristina; Herrera,Raírys Cravo; Porto,Jorge Marcelo Padovani; Vargas,Daiane Peixoto; Alves,Eduardo.
This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passifloraceae; Callogenesis; Electromicroscopy; Optic microscope; Tissue culture.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851

Methanogenesis in an impacted and two dystrophic coastal lagoons (Macaé, Brazil)
Provedor de dados: 52
Autores: Furtado,André Luiz dos Santos; Casper,Peter; Esteves,Francisco de Assis.
This study investigated the methanogenic activity in sediment of the Imboacica (human impacted), Cabiúnas and Comprida coastal lagoons in Rio de Janeiro State (Brazil). Methane was not detected in water and sediment samples from the three lagoons. The measured nutrient concentrations in the pore-water indicated that methanogens activity was not limited by nutrients. Methanogenic activity was not detected under in vitro conditions, indicating that terminal organic carbon mineralisation via methanogenesis was negligible for the top 6 cm of sediment at the sampling time.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal lagoon; Methane; Sediment; Nutrients.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200011

Acidic polysaccharides from Psidium cattleianum (Araçá)
Provedor de dados: 52
Autores: Vriesmann,Lúcia Cristina; Petkowicz,Carmen Lúcia de Oliveira; Carneiro,Paulo Irajara Borba; Costa,Maria Eugênia; Beleski-Carneiro,Eliana.
The aim of this work was to study the acidic polysaccharides of Psidium cattleianum. Pectic fractions were obtained by successive extractions with water, EDTA and sodium carbonate (4 and 25ºC). Monosaccharide composition, total carbohydrates, uronic acids and protein contents were determined for each fraction. The yield of water and EDTA-soluble fractions and high content of uronic acid were consistent with the presence of pectins probably arising from the middle lamellae. On the other hand, sodium carbonate-soluble pectins had a higher neutral sugar content, indicating highly branched polysaccharides in these fractions, consistent with pectins from cell wall.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectins; Acidic polysaccharides; Psidium cattleianum; Cell wall.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200001

Fed batch enzymatic saccharification of food waste improves the sugar concentration in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae H058
Provedor de dados: 52
Autores: Yan,Shoubao; Yao,Jianming; Yao,Liming; Zhi,Zhijun; Chen,Xiangsong; Wu,Jingyong.
The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation of the hydrolysates by Saccharomyces cerecisiae H058 were studied in this work. The optimum batch enzymatic conditions were found to be saccharification pH of 4.5, temperature of 55!, glucoamylase concentration of 120 u/g, α-amylase concentration of 10 u/g, solid-liquid ratio of 1: 0.75 (w/w). Fed batch hydrolysis process was started with a solid-liquid ratio of 1: 1 (w/w), with solid food waste added at time lapse of 2 h to get a final solid-liquid ratio of 1: 0.5 (w/w). After 4 h of reaction, the reducing sugar concentration reached 194.43 g/L with a enzymatic digestibility of 93.12%. Further fermentation of the batch and fed batch enzymatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food waste; Enzymatic hydrolysis; Hydrolysates; Fermentation; Ethanol production.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200002

Serological Identification of Virus in Watermelon Production Fields in the Tocantins State
Provedor de dados: 52
Autores: Aguiar,Raimundo Wagner de Souza; Rodrigues,Adelmo; Portella,Augustus Caeser Franke; Lopes,Magnolia de Mendonça; Lima,Mirtes Freitas; Resende,Renato de Oliveira; Nagata,Tatsuya.
Watermelon (Citrullus lanatus) cultivated in almost all tropical and subtropical regions of the world, has its largest output in China, and then, according to FAO data, Turkey, Iran and Brazil, being one of the main crops cultivated in State of Tocantins, Brazil. In this work was investigated the occurrence and distribution of the watermelon viruses, totaling 752 samples taken in a stratified experimental design in four representative regions of production: Gurupi (150), Lagoa da Confusao (232), Formoso do Araguaia (265) and Porto Nacional (105). The sampling and collecting the leaves of plants with the presence of symptoms were performed once a week during the entire cultivation cycle. As a result, were observed by Dot-ELISA method, different types of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Watermelon; Citrullus lanatus; Potyvirus; Cucumovirus; Tospovirus; Serological assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200192

Processing of bluefish, Pomatomus saltatrix using natural smoke flavouring as coadjuvant
Provedor de dados: 52
Autores: Gonçalves,Alex Augusto; Prentice-Hernández,Carlos.
The bluefish (Pomatomus saltatrix) constitutes the main fishing resource in Rio Grande do Sul (Brazil) coast in the autumn, winter and spring months. Considering that this fish is highly perishable, to have its shelf life increased an adequate treatment and processing are needed as soon as its capture occurs. Its use in the elaboration of a smoked product can represent considerable economic growth, if compared to its current "in natura" or frozen commercialization. A bluefish base smoked product was elaborated using natural smoke flavouring (known commercially as liquid smoke) defining the adjusted technological characteristics during the processing. The best form of liquid smoke application (immersion or sprinkling) was chosen sensorially by hedonic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bluefish; Processing; Smoked fish; Liquid smoke.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100006

Bulliform cells in Loudetiopsis chrysothrix (Nees) Conert and Tristachya leiostachya Nees (Poaceae): structure in relation to function
Provedor de dados: 52
Autores: Alvarez,Juliana Magalhães; Rocha,Joecildo Francisco; Machado,Silvia Rodrigues.
This work reports anatomic and ultrastructural characteristics of bulliform cells in Loudetiopsis chrysothrix (Nees) Conert and Tristachya leiostachya Nees. Both the species presented leaf rolling under water stress. The main characteristics observed in these cells were: periclinal wall thinner than the adjacent epidermal wall; abundance of pectic substances in cuticular layer; sinuous anticlinal walls with ramified plasmodesmata; vacuome formed by a developed vacuole or innumerous small vacuoles; abundance of phenolic substances and oil drops. These characteristics suggested the involvement of bulliform cells in the mechanism of foliar involution in the studied species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Bulliform cells; Cerrado; Poaceae; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100014

Phytotoxin produced by Bipolaris euphorbiae in-vitro is effective against the weed Euphorbia heterophylla
Provedor de dados: 52
Autores: Barbosa,Aneli M.; Souza,Cristina G. M.; Dekker,Robert F. H.; Fonseca,Rafael C.; Ferreira,Dalva T.
Four virulent strain isolates of the fungus, Bipolaris euphorbiae (previously identified as a Helminthosporium sp.), isolated from host plants in four states within Brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the Brazilian weed, Euphorbia heterophylla, commonly found growing among soyabean crops. Only one isolate, B. euphorbiae Strain I (EUPH petropar from Mato Grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° C on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. Phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. The addition of nitrogen source (yeast extract, peptone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bipolaris euphorbiae; Euphorbia heterophylla; Phytotoxin production; Phytotoxin stability.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200016

Selection for grain yield and quality in segregating generations of wheat
Provedor de dados: 52
Autores: Andrade,Alessandro Ramos de; Riede,Carlos Roberto; Scholz,Maria Brígida dos Santos; Destro,Deonisio; Fonseca,Ines Cristina de Batista.
The objective of the work was to combine simultaneous selection for grain yield and technological quality in elite segregating populations of wheat developed by the breeding program of IAPAR. Thirteen populations were chosen according to their potentiality of genetic variability in the F2 generation of the 1997 crop season. One hundred heads were selected from each population and sown as F3 head-rows in the following season. Visual selection of individual head-rows or lines was done for yield potential and maturity. Seeds of the selected lines were evaluated for Sedimentation Values as well as Protein Content. The populations were identified as C1 to C13, representing the different crosses. The number of F2:3 lines selected within populations varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Technological quality; Grain yield; Segregating generations.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200010

Chemical composition of flours made of residues from the king palm (Archontophoenix alexandrae) industry
Provedor de dados: 52
Autores: Vieira,Manoela Alano; Podestá,Rossana; Tramonte,Karina Cardoso; Amboni,Renata Dias de Mello Castanho; Simas,Karina Nunes de; Avancini,Sandra Regina Paulon; Amante,Edna Regina.
Residues from King palm (Archontophoenix alexandrae) processing were used for the production of flours, which were then chemically characterized. The protein content in these flours ranged from 3.62 to 9.75 g/100g and was higher in sifted leaf flour (SLF). The dietary fiber contents varied from 64 to 72 g/100g. These values were high when compared to those of flours used in human nutrition. Analysis of anti-nutritional factors showed phytate contents to be below the levels that affected the bioavailability of minerals in human diet. Tannin contents were compatible with those found in legumes, between 0 and 2000 mg/100g. These flours showed high mineral content, which suggested a possibility for them to be used as food supplement. However, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Archontophoenix alexandrae; Residues; Flour; Chemical analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400021

Soft Collagen-Gelatine Sponges by Convection Drying
Provedor de dados: 52
Autores: Meyer,Michael; Trommer,Kristin.
This study showed that thermally labile fibrillar collagen could be processed continuously in combination with gelatine as foaming additive by convection drying. The procedure led to stable sponges with similar structural and physical properties as found for freeze-dried collagen samples. The fibrillar collagen remained native, while gelatine acted as foaming additive. The absorbency of the sponges was improved by opening the surface with abrasives. A use as medical device with hemostyptic properties would be possible.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen; Gelatine; Convection drying; Sponge.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100109

Changes in diapause related gene expression pattern during early embryonic development in HCl-treated eggs of bivoltine silkworm Bombyx mori (Lepidoptera: Bombycidae)
Provedor de dados: 52
Autores: Sasibhushan,Sirigineedi; Ponnuvel,Kangayam M.; Vijayaprakash,Nanjappa B.
Investigation of differential expression of diapause related genes (five metabolic, five heat shock protein and one translational regulatory) in HCl-treated (non-diapause) and untreated (diapause) eggs of B. mori during early embryogenesis (up to 48h following oviposition) revealed the up-regulation of sorbitol dehydrogenase upon HCl treatment, indicating increased glycogen synthesis for further embryonic development but, down-regulation of phosphofructo kinase gene expression after 18h of oviposition indicating an arrest of glycerol and sorbitol conversion. The expression of poly A binding protein gene expression was higher upon HCl treatment, revealing the initiation of translation. The expression levels of other genes analyzed did not vary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hibernation; Acid treatment; Metabolic genes; Heat shock protein genes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100001

Immunomodulation of Juvenile Pacu, Piaractus mesopotamicus, by Different β(1-3)(1-6)-D glucan Products
Provedor de dados: 52
Autores: Sabioni,Rafael Estevan; Zanuzzo,Fábio Sabbadin; Cyrino,José Eurico Possebon.
Abstract Stress in intensive fish farming hamper immune function of fish and cause losses by disease outbreaks, a situation that can be minimized, but cannot be completely circumvented, by the use of immunomodulators. Addition of immunomodulators to aquafeeds has thus become a common practice. β-glucan (BG) is one of most studied and effective immunomodulators, aquaculture purposes included. Extracted from cell walls of bacteria, fungi and selected cereals, BG activity depends on the source and extraction methods. This study evaluated effects of two BG products (BG1 and BG2), extracted from Saccharomyces cerevisiae under varying extraction methods and with different immune activity, on the feeding of pacu Piaractus mesopotamicus juveniles. BG1 provided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunostimulant; Aquaculture; Stress; Aeromonas hydrophila; Leukocytes; Lysozyme.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100410

A Trajetória de "Arquivos de Biologia e Tecnologia", Publicação que Marcou Época na História da Ciência Brasileira
Provedor de dados: 52
Autores: Bacila,Metry.
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500002

The abiotic environment of Heliamphora nutans (Sarraceniaceae): pedological and microclimatic observations on Roraima Tepui
Provedor de dados: 52
Autores: Adlassnig,Wolfram; Pranji,Kornelija; Mayer,Edith; Steinhauser,Georg; Hejjas,Flora; Lichtscheidl,Irene K.
The aim of this study was the study of the abiotic environment of the carnivorous pitcher plant Heliamphora nutans (Sarraceniaceae), including the microclimate and the geochemistry of the soil of the growing sites on Roraima Tepui and discuss their relevance within the recent model of carnivorous plant ecology. The soil was peaty and low in nutrients. The microclimate on the site was very balanced, with moderately cool temperatures, a constant high humidity and very low wind speed. Heliamphora was not exposed to any recognizable climatic stress. Previous macroclimatic measurements reflected the growth conditions of Heliamphora only incorrectly, since humidity decreased drastically with height. The apparent conflict with the common model of carnivorous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carnivorous plants; Microclimate; Soil; Niching; Competition; Cultivation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200022

Hydroxypropylcellulose controlled release tablet matrix prepared by wet granulation: effect of powder properties and polymer composition
Provedor de dados: 52
Autores: Teixeira,Antonio Zenon Antunes.
The aim of this study was to attain 100% drug release of caffeine after 24 h from hydroxypropylcellulose (HPC) tablet matrices and to investigate the effect of co-excipient. Physical properties of the powders were evaluated and suggested for a wet granulation process. The tablet containing caffeine was formulated by different weight ratios of hydrophilic polymers. The results of polymer evaluation confirmed that the increase of HPC level with the same drug content significantly decreased the rate of drug release. The presence of co-polymer excipients carboxymethylcellulose (CMC) and polyvinylpyrrolidone (PVP) in the tablet matrix was also investigated. The release rate was also controlled by low levels of CMC (&lt;10%) while PVP did not show any...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Controlled release; Caffeine; Hydroxypropylcellulose (HPC); Polyvinylpyrrolidone (PVP); Carboxymethylcellulose (CMC); Lactose.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100021

Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
Provedor de dados: 52
Autores: Bezerra,Raquel Pedrosa; Borba,Fernanda Katharine Sousa Lins; Moreira,Keila Aparecida; Lima-Filho,José Luis; Porto,Ana Lúcia Figueiredo; Chaves,Adilson Castro.
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylases; Aqueous two-phase systems; Bacillus subtilis; Partition; Poly (ethylene glycol).
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003

Impact of Equine Chorionic Gonadotropin Associated with Temporary Weaning, Estradiol Benzoate, or Estradiol Cypionate on Timed Artificial Insemination in Primiparous Bos Indicus Cows
Provedor de dados: 52
Autores: Souza,Andre Luis Bastos; Segui,Marcio Saporski; Kozicki,Luiz Ernandes; Weiss,Romildo Romulado; Abreu,Ana; Bertol,Melina Andreia Formighieri; Oliveira,Danilo Martins Amadori.
The study aimed to determine the impact of equine chorionic gonadotropin (eCG) associated with different timed artificial insemination (TAI) protocols on the pregnancy rate (PR) in Bos indicus cows previously treated with progesterone. Five hundred and fifty-seven primiparous cows were subjected to the following treatments: on day 0 (d0), GeCGTW (group equine Chorionic Gonadotropin+Temporary Weaning;n=178) received 0,558 g intravaginal progesterone (P4)+1.0 mg of estradiol benzoate (EB) (IM); on d8 (P4 removal+0,075 mg D-cloprostenol + 400 IU eCG + TW for 48 h); on d10, TAI + calves return to dam; GeCGEB (group equine Chorionic Gonadotropin+Estradiol benzoate; n=176) the same as GeCGTW without TW + application of 1.0 mg of EB on d9; GeCGEC (group equine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef catlle; Equine Chorionic Gonadotropin; Fixed time artificial insemination.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100305

Evaluation of Gamma Irradiation Effect on the Oxidative Stress Factors in Septic Rats Treated With Iranian Plant Essential Oils
Provedor de dados: 52
Autores: Fatemi,Faezeh; Yazdi,Atefeh Jalali; Ghanooni,Shirin; Zolfaghari,Mohammad Reza.
ABSTRACT Cuminum cyminum L. (CM), Zataria multiflora Boiss. (ZM) and Mentha piperita L. (MP) are traditional medicinal plants with various pharmacological properties. This study was designed to assess the role of gamma irradiation -a modern decontamination method- in hepatoprotective effects of their essential oil (E.Os) in septic rats induced by experimental cecal ligation and puncture (CLP) model. The rats were divided into 20 groups; sham-operated (SOP); CLP; CLP + CM, ZM and MP (E.Os) (100 &amp; 200 mg/kg b.w) and CLP + gamma irradiated (10 and 25 kGy) E.Os (100 &amp; 200 mg/kg b.w) as treatment groups. All E.Os were injected i.p immediately after sepsis induction. 24 hour after CLP, the rats were sacrificed and the liver tissue was examined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oils; Oxidative stress; Antioxidant; CLP; Gamma irradiation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100312

Benefits Associated with the Interaction of Endophytic Bacteria and Plants
Provedor de dados: 52
Autores: Santos,Miriam Langner dos; Berlitz,Diouneia Lisiane; Wiest,Shana Leticia Felice; Schünemann,Rogerio; Knaak,Neiva; Fiuza,Lidia Mariana.
ABSTRACT The endophytic bacteria belong to a larger group of microorganisms that have their life-cycle partly or entirely inside the plant and are located in intra and inter-cellular spaces or in the vascular tissue. These bacteria can be found colonizing aerial parts or roots. This review aims to analyze the colonization strategies of endophytic bacteria through interaction with plants, as well as to highlight the metabolic influence of these organisms in plant tissues, which result in physiological and biochemical changes. Depending on the different mechanisms used internally to colonize a plant, these microorganisms are called obligate, facultative, or passive endophytes. Phytostimulation, biofertilization and biological control are mechanisms that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofertilization; Biological control; Colonization strategies; Microorganisms; Phytostimulation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100200

Influence of Plant Growth Regulators on In Vitro Shoot Multiplication and Plantlet Formation in Cassia angustifolia Vahl
Provedor de dados: 52
Autores: Siddique,Iram; Bukhari,Najat Abdullah Wahab; Perveen,Kahkashan; Siddiqui,Iffat.
ABSTRACTAn effective and improved plant regeneration system was successfully developed using shoot tip explants taken from a two years old mature plant of Cassia angustifolia. The effect of different cytokinins, [6-benzyladenine (BA), Kinetin (Kin) and thidiazuron (TDZ)] at different concentrations (0.5-10 µM) were evaluated as augmented with Murashige and Skoog (MS 1962) medium. Among all the cytokinins tested, TDZ (5.0 µM) was optimum in inducing multiple shoots as compared to BA and Kin. The rate of shoot multiplication was increased when optimal concentration (5.0 µM) of BA and Kin was tested with different concentration (0.1-1.0 µM) of Indole-3- acetic acid (IAA). Among all the combinations tested, the maximum rate of shoot multiplication was obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acclimatization; Growth regulator; Indole-3- butyric acid; Shoot tip; Senna.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500686

Isolation and characterization of selenate resistant mutants of Acremonium chrysogenum
Provedor de dados: 52
Autores: Vialta,Airton; Catani,Cleide F; B. Junior,Renato; Azevedo,João L.
Mutants unable to convert exogenous sulfate to sulfite were isolated using the toxic analogue selenate. Three of twenty-eight isolated mutants were chromate sensitive. They showed a possible lesion in the gene that codes the ATP sulfurylase. The others were chromate resistant, and probably had a lesion in one or both of the genes that code the sulfate permease. Methionine increased the resistance levels to selenate. In addition, the frequency of spontaneous mutants obtained in a medium containing methionine was higher (between 2.4 x 10-6 and 18.0 x 10-6) than that obtained using a medium without any intentional source of sulfur (between 0.7 x 10-6 and 5.0 x 10-6). The original strain, as well as the mutants, were able to grow in a sulfur-free liquid medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Selenate resistant mutant; Acremonium chrysogenum; Cephalosporin C.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300016

Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
Provedor de dados: 52
Autores: Choi,Seung Kug; Park,Sunmi; Moon,Hyun-Seuk.
We utilized subcutaneous (SC)- and omental (OM)-derived human primary adipocytes (hPA) from obese male, and investigated whether synthetic analog of leptin, metreleptin, may regulate lipolysis via translocation of STAT3 to the nucleus. We observed that 50 ng/mL of metreleptin increases STAT3 phosphorylation in both SC- and OM-derived hPA. Importantly, we found for the first time that metreleptin is capable of trans-locating STAT3 to the nucleus and STAT3 blockade inhibits metreleptin-induced lipolysis. Our initial data provide novel insights into the role of STAT3 as probable mediator of the action of metreleptin in regulating metabolism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metreleptin; Human primary adipocyte; STAT3; Differentiation; Lipolysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100309

State-of-the-Art Impacts of Smart Grid in the Power Systems Operation and Expansion Planning
Provedor de dados: 52
Autores: Oliveira,Gabriel Apoena de; Muthemba,Leonel João; Unsihuay-Vila,Clodomiro.
ABSTRACT In this article, a state-of-the-art review on the impacts of Smart Grid in the operation and planning of the expansion of the Electric Power System is presented. The concept of Smart Grid is increasingly integrated in conventional networks, and the impact that this new technology generates on operation and planning must be investigated. Thus, in this article a survey of the main scientific publications was made with the purpose of determining the impacts and methodologies most used in this analysis. Efforts to analyze impacts are large because of the high degree of uncertainty of this new technology added to the problem. After the bibliographic survey, it was concluded that Robust Optimization and Genetic Algorithms are methodologies that are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: State-of-the-art; Methodology; Smart grid; Impact; Power systems expansion planning; Power systems operation planning.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200210

Bone Marrow Mesenchymal Stem Cells Regulate Coagulation and Inflammation Together in Methotrexate Induced Lung Injury Rat Model
Provedor de dados: 52
Autores: Haggag,Nawal Zakaria; El-Shinnawy,Nashwa Ahmed; Abd-Elhalem,Sahar Sobhy; Rashed,Laila Ahmed.
Abstract Clinical research has shed the light on the relation between coagulation and inflammation. Coagulation cascade is activated in lung injury resulting in thrombotic and fibrotic lesions. Such a cascade is initiated by inflammation, then the two systems intense each other. New therapies that modulate coagulation and inflammation will be more successful than therapies targeting only one of them. Mesenchymal stem cells showed anti-inflammatory functions in animal models. The role of mesenchymal stem cells in methotrexate induced lung injury model was evaluated, but no studies scoped on the role of stem cells in coagulation associated with inflammation in such models. This study focuses on the therapeutic role of mesenchymal stem cells against the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulation; Inflammation; Lung injury; Mesenchymal Stem Cells; Methotrexate.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100307

Nitrogen forms, pH and total carbon in a soil incubated with treated sewage effluent
Provedor de dados: 52
Autores: Fonseca,Adriel Ferreira da; Herpin,Uwe; Dias,Carlos Tadeu dos Santos; Melfi,Adolpho José.
In this study, an experiment under controlled conditions was carried out to determine the effects of secondary-treated sewage effluent (STSE) application on soil nitrogen concentrations (mineral and total), total carbon and soil pH. The soil and STSE used were collected at Lins, São Paulo State, Brazil. A completely randomized design was used, in completed factorial 4x11 (weekly application rates of 0, 100, 150 and 200 mL STSE per kg soil; and, eleven soil incubation periods from 0 to 10 weeks) with four replicates. The STSE was applied simulating common surface irrigation. Seven days after each incubation period, ammonium and nitrate contents were determined. Additionally, pH and total carbon and nitrogen contents were measured in the soil after 10 weeks....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Treated sewage effluent; Nitrogen; Carbon; Tropical soil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500001

Identification of yeasts Isolated from processed and frozen cocoa (Theobroma cacao) pulp for wine production
Provedor de dados: 52
Autores: Trindade,Rita de Cássia; Resende,Maria Aparecida de; Barreto,Eriana Gomes Serpa; Mendes,Taniella de Carvalho; Rosa,Carlos Augusto.
The alternative use of cocoa (Theobroma cacao) for wine production was tested. The pulp samples, obtained from Formosa farm, Itacaré, Brazil, were diluted, homogenized and inoculated on Sabouraud dextrose agar medium (SDA) and incubated at 28º C for 5-8 days. Selected colonies were tested for the ability to ferment cocoa pulp and divided into fermentative, non-fermentative and weak/late fermentative species. Isolates characterized as fermentative were further tested in a small-scale wine production plant and identified. Species from the genus Brettanomyces constituted the main fermentative yeasts, with the exception of two Kloeckera apis samples. The final wine product was normally pale or clear, making clarification unnecessary, and with a sweet or dry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Cocoa pulp; Fermentation; Wine production; Brettanomyces sp.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300013

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro
Provedor de dados: 52
Autores: Coelho,Alexandre Rodrigo; Celli,Marcos Giovani; Ono,Elisabete Yurie Sataque; Wosiacki,Gilvan; Hoffmann,Fernando Leite; Pagnocca,Fernando Carlos; Hirooka,Elisa Yoko.
Taking into account the preliminary antagonistic/biodegradation property showed by Pichia membranifaciens and Sporobolomyces roseus, which decreased the initial patulin concentration of 588.4 to 290.0 µg/mL, ability of P. ohmeri 158 in biocontrol against Penicillium expansum and patulin decrease in vitro was performed. The culture supernatant of P. ohmeri 158 was effective against 66.17% micelial growth, indicating antibiosis related with the killer phenomenon. The initial patulin concentration of 223 µg in the presence of P. ohmeri 158 cells was decreased over 83% of the original concentration, when incubated at 25ºC/2 days and &gt; 99% after 5 days incubation time, with undetectable patulin level after 15 days. The initial pH 4.0 decreased to pH 3.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium expansum; Antagonist yeasts; Biocontrol; Patulin; Degradation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400019

Detection of Diarrheagenic Escherichia coli in Bovine Meat in the Northern Region of Paraná State, Brazil
Provedor de dados: 52
Autores: Pelayo,Jacinta Sanchez; Elias Junior,Antonio Roberto; Lima,Nicole Ribeiro de; Navarro,Armando; Rocha,Sérgio Paulo Dejato da.
Abstract Ground bovine meat is commonly consumed by the population of Brazil. However, it constitutes an excellent medium for the multiplication of microorganisms due to available nutrients and handling practices prior to consumption. Here, we examined 100 samples of ground beef for the presence of diarrheagenic Escherichia coli (DEC) pathotypes by PCR, and characterized isolates by analyzing their adherence to HEp-2 cells, serotype, antimicrobial susceptibility, and phylogeny. Enteroaggregative E. coli was detected in five (5%) meat samples, Shiga toxin-producing E. coli in three (3%), and atypical enteropathogenic E. coli in two (2%). According to the phylogeny, six isolates (60%) were classified in group A, two (20%) in group B1, and two (20%) in group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrheagenic Escherichia coli; Ground beef; Gastroenteritis; Serotyping.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100502

Ketoconazole- and fluconazole-induced embryotoxicity and skeletal anomalies in wistar rats: a comparative study
Provedor de dados: 52
Autores: Amaral,Vanessa Cristiane de Santana; Nunes Junior,Guilhermino Pereira.
Ketoconazole and fluconazole are two broad-spectrum azole antifungals used for the treatment of superficial and systemic mycoses. Embryotoxicity and teratogenicity have been reported in some studies when those drugs are administered at high doses to pregnant rats. The aim of this study was to present a comparative study of embryotoxic effects as well as the skeletal anomalies in fetuses of Wistar rats which received ketoconazole and fluconazole at teratogenic doses on gestational days (GD) 6 through 15 (organogenesis period). On gestational day (GD) 21, the dams were euthanized and examined for standard parameters of reproductive outcome. Fetuses were stained with alizarin red and the bones of the head, trunk, forelimb and hindlimb were examined for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ketoconazole; Fluconazole; Maternal toxicity; Embryotoxicity; Skeletal malformations.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600010

Inter-annual variability of planktonic copepods in a tropical bay in southeastern Brazil
Provedor de dados: 52
Autores: Dias,Cristina de Oliveira; Bonecker,Sérgio Luiz Costa.
The aim of this work was to study the structure of the copepod community in relation to hydrographic conditions in Espírito Santo Bay, from 1993 to 1997. Zooplankton samples were obtained in the horizontal tows four times a year, using a 200 µm net. Temperature, salinity and dissolved oxygen were determined in the water samples. The overall mean density of the copepods was 9,085 ind.m-3 with the peaks exceeding 15,000 ind.m-3 in the summer and autumn during the first three years (1993 - 1995). The PCA analysis was used to relate the environmental conditions with the distribution of the copepods. The increases in the abundant species, namely Acartia lilljeborgi, Oithona hebes, Oithona oculata, Paracalanus quasimodo, Parvocalanus crassirostris and Euterpina...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copepoda; Crustacea; Spatial-temporal variation; Espírito Santo Bay; Brazil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400011

Effect of salt on color and warmed over flavor in charqui meat processing
Provedor de dados: 52
Autores: Youssef,Elza Y.; Garcia,Carlos E. Rocha; Shimokomaki,Massami.
A combination of salt (NaCl) high concentration and curing salt was investigated for their role in warmed-over flavor (WOF) and color changes during charqui meats processing. WOF was measured by TBARS method in uncured charqui meat (CH) and in cured charqui known in Brazil as Jerked beef (JB). WOF occurred substantially in CH and sodium nitrite was able to inhibit 40-45% (p&lt;0.05) in JB samples stored for 30 days. Color parameters also changed as evaluated by CIELAB system. The a*/b* ratio showed that CH samples presented brown color indicating the formation of metmyoglobin (Fe3+) whilst JB samples presented deep red color an indication of nitrosylmyoglobin (Fe2+) formation. Under cooking, a*/b* ratio indicated the presence of denatured metmyoglobin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intermediate moisture meat product; Jerky meat; Charqui meat; Cured meat; Rancidity; Warmed over flavor inhibition.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400014

Comparison of the Performance of the Grid- Connected Photovoltaic System Installed In the Brazilian Cities: Blumenau - SC and Curitiba- PR
Provedor de dados: 52
Autores: Stedile,Rafael; Tonin,Fabianna Stumpf; Urbanetz Junior,Jair.
ABSTRACT This is a study that contemplates the analysis of the main characteristics of 8 grid connected photovoltaic systems (GCPVS), 3 of which are located in the city of Curitiba-PR and 5 located in the city of Blumenau-SC. Data were collected on irradiation in the horizontal plane, generated energy and rainfall, being the first two to calculate the merit indexes, such as Yield, Performance Ratio and Capacity Factor - of those on grid connected Photovoltaic systems.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grid Connected Photovoltaic Systems; Merit Indices; Microgeneration.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200214

Enzymatic programs for broilers
Provedor de dados: 52
Autores: Sorbara,José Otávio Berti; Murakami,Alice Eiko; Nakage,Eliane Saiuri; Piracés,Francisco; Potença,Alexandra; Guerra,Rafael Lachinski Holanda.
Two trials were conducted on floor pens to evaluate the performance of broilers fed with diets contained different carbohydrases enzyme programs at different ages. Trial 1- The body weight gain (BWG) was affected by the Negative Control (NC) treatment (P&lt;0.05). However, when the birds were fed with the NC diet + Amilase (AMY)+Xilanase (XYL), the BWG improved and reached the same level as the Positive Control (PC). Trial 2- The birds received enzymes supplementation in the total phase and others only in the grower phase.The ME reduction by 120 kcal/kg increased the feed conversion ratio (FCR) (P&lt;0.05) when compared to PC and none of the enzyme programs was able to recover the performance. It can be concluded that it is possible to supplement with AMY...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Carbohydrase; Enzymes; Amylase; Xylanase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700030

Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli
Provedor de dados: 52
Autores: Niu,Li-Xi; Li,Jia-Yue; Ji,Xue-Xue; Yang,Bin-Sheng.
Human enterokinase (synonym: enteropeptidase, EC 3.4.21.9) light chain (hEKL) gene was designed and artificially synthesized with built-in codon blas towards Escherichia colicodon preference. The synthetic hEKL gene was cloned into prokaryotic expression vector pMAL-s and transferred into the expression strain E. coli BL21 (DE3). Recombinant hEKL protein with a maltose binding protein (MBP) tag was expressed at high levels in soluble form, which yielded about 42% of the total cellular protein. The target protein was then purified to the homogeneity (&gt; 95%) by affinity chromatography. The peptide substrate GST-Melittin with enterokinase recognition site was completely cleaved by the purified MBP-hEKL at the molar ratio of 1:5000 (enzyme:substrate)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human enterokinase light chain (hEKL); Fusion expression; Affinity purification; Activity analysis; Enzymatic cleavage.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200154

Mortality of Oryzophagus oryzae (Costa Lima, 1936) (Coleoptera: Curculionidae) and Spodoptera frugiperda (J E Smith, 1797) (Lepidoptera: Noctuidae) Larvae Exposed to Bacillus thuringiensis and Extracts of Melia azedarach
Provedor de dados: 52
Autores: Berlitz,Diouneia Lisiane; Azambuja,Aline Oliboni de; Sebben,Alessandra; Oliveira,Jaime Vargas de; Fiuza,Lidia Mariana.
Oryzophagus oryzae (Costa Lima 1936) (Coleoptera: Curculionidae) and Spodoptera frugiperda (J E Smith, 1797) (Lepidoptera: Noctuidae) cause important crop losses in southern Brazil. Control is possible by the use of the bacteria Bacillus thuringiensis and extracts of Melia azedarach. This study aimed to evaluate the mortality, in vivo, of O. oryzae and S. frugiperda submitted to two isolates of B. thuringiensis and the aqueous extract of M. azedarach. The LC50 for O. oryzae due to bacteria was 5.40μg/mL (Bt 2014-2) and due to plant extract 0.90μg/mL. For S. frugiperda, the Bt 1958-2 bacterial suspension (1.10(10)UFC/mL) caused a 100% of corrected mortality, showing that the purified Cry proteins caused a CL10 of 268μg/mL five days after the treatments, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative pest control; Entomopathogenic bacteria; Insecticidal activity; Botanical insecticidal.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500012

Antioxidant potential of tomatoes cultivated in organic and conventional systems
Provedor de dados: 52
Autores: Borguini,Renata Galhardo; Bastos,Deborah Helena Markowicz; Moita-Neto,José Machado; Capasso,Fernanda Sobral; Torres,Elizabeth Aparecida Ferraz da Silva.
The objective of the study was to compare the effect of organic and conventional cultivation on the antioxidant compound content and antioxidant activity of the Carmen tomato cultivar. Tomatoes were analyzed regarding ascorbic acid, phenolic compounds, lycopene content and antioxidant activity. Organic tomatoes presented higher content of ascorbic acid and total phenolics (641.39 and 4466.66 mg/100 g EAG on dry wt basis) than did the conventional tomatoes (510.16 and 3477.50 mg/100 g EAG on wt dry basis, respectively). There was no difference in lycopene concentrations between the organic and conventional. The ether, alcohol and aqueous extracts obtained from the tomatoes were subjected to the DPPH test and the β-carotene/linoleic acid system assay. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant activity; Ascorbic acid; Lycopene; Phenolic compounds.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400001

Hexavalent chromium reduction by immobilized cells of Bacillus sphaericus AND 303
Provedor de dados: 52
Autores: Pal,Arundhati; Datta,Sudeshna; Paul,Amal K.
Bacillus sphaericus AND 303, a Cr(VI)-resistant and reducing bacterium reported from serpentine outcrops of Andaman was evaluated for Cr(VI) reduction using immobilized cells under batch culture. Screening of inert matrices for entrapment of whole cells indicated that polyvinyl alchohol-alginate was the most effective one reducing 87.5% of 20 µM Cr(VI) in 24 h. The rate of chromate reduction was dependent on initial Cr(VI) and biomass concentrations. The PVA cell beads were recycled three times without cell leakage and disintegration. The reduction efficiency was improved in the presence of glucose and glycerol as electron donors leading to complete reduction. However, the presence of additional metal ions was inhibitory to Cr(VI) reduction. It could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus sphaericus; Immobilized cells; PVA alginate; Cr(VI) reduction: Bioremediation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300019

Culture conditions for production of 2-1-β-D-Fructan-fructanohydrolase in solid culturing on chicory (Cichorium intybus) roots
Provedor de dados: 52
Autores: Pandey,Ashok; Kavita,Poomarathil; Selvakumar,Ponniah.
Investigations were carried out to optimize the culture conditions for the production of b-D-fructan-fructanohydrolase by an indigenously isolated bacterial culture of Staphylococcus sp. RRL8. Experiments were carried out in solid culturing using chicory roots (powdered) as the source of carbon, which was supplemented, with corn steep liquor and potassium dihydrogen phosphate. A number of process parameters, like period of cultivation, initial moisture content in the substrate and temperature of incubation were optimized. Maximum extra-cellular enzyme was produced when fermentation was carried out at 30ºC for 24 h using chicory roots with 60% initial moisture. Supplementation of the substrate with additional carbon source (except with sucrose) resulted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fructan-fructanohydrolase; Inulinase; Solid state fermentation; Staphylococcus sp.; Chicory.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200010

A Computational Framework for Daily Power Flow Analysis Considering Photovoltaic Distributed Generation
Provedor de dados: 52
Autores: Gurski,Erico; Moraes,Fillipe Alexandre; Fritzen,Paulo Cícero; Benedito,Raphael Augusto de Souza.
Abstract In Brazil, the demand for the acquisition and installation of photovoltaic systems has grown exponentially and, with that, the importance of studies considering their effects on the distribution grids power flow also grows. Due to the intermittent nature of photovoltaic generation, it is important to approach the analysis in a discrete way, taking into account the changes in the power injections during the studied period. Thus, this article presents the daily analysis of the effects of photovoltaic generation on the distribution system through the computational implementation of a discretized power flow routine. The implemented routine can also analyze the effects of other distributed generations on distribution grids, whether those grids are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distributed Generation; Power Flow; Photovoltaic Systems.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200207

Characterization of Colletotrichum lindemuthianum isolates using differential cultivars of common bean in Santa Catarina State, Brazil
Provedor de dados: 52
Autores: Gonçalves-Vidigal,Maria Celeste; Thomazella,Claudia; Vidigal Filho,Pedro Soares; Kvitschal,Marcus Vinícius; Elias,Haroldo Tavares.
In 2003 and 2004, 32 isolates of Colletotrichum lindemuthianum obtained from the infected plants of field-grown common bean (Phaseolus vulgaris L.) in Santa Catarina state, Brazil were analyzed based on the virulence to 12 differential cultivars of Phaseolus vulgaris L.. Thirteen distinct races were identified, six of which had not been reported previously in Santa Catarina. This is the first report of the occurrence of 67, 83,101,103,105, and 581 races of C. lindemuthianum. Race 65 was most common (34%). All the isolates were compatible to the cultivars Michelite and Mexico 222. Some isolates infected not only differential cultivar of Mesoamerican origin, but also the ones of Andean origin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthracnose; Common bean; New races; Phaseolus vulgaris L.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500002

Cardioprotection of Rosuvastatin Pre-conditioning on Myocardial Ischemia / Reperfusion Injury in a Rat Model
Provedor de dados: 52
Autores: Zhang,Pei-Xi; Zhang,Hong-Xia; Hou,Dai-Liang; Xiao,Zhi-Chao; Zhou,Cheng-Yun.
Abstract This study aimed to investigate the cardioprotection of rosuvastatin pre-conditioning (R-Pre) in a rat model of myocardial ischemia / reperfusion (I/R). Male SD rats were assigned into three groups: sham group, I/R group and R-Pre group. Rats in I/R group and R-Pre group received ischemia for 30 min and reperfusion for 2 h. In R-Pre group, rats received intragastrical administration with rosuvastatin at 5 mg/kg once daily for 1 week. After 2-h reperfusion, the cardiac function was detected by ultrasonography; the blood was collected for biochemical analysis; the heart was collected for the TUNEL staining and immunohistochemistry for Bcl-2 and Bax. Our results showed rosuvastatin pre-conditioning for 1 week could significantly reduce the infarct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial ischemia/reperfusion; Rosuvastatin; Oxidative stress; Apoptosis; Pre-conditioning.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100311

Use of Frozen Leaves for Morpho-anatomical Characterization of Nectandra megapotamica (Spreng.) Mez., Lauraceae
Provedor de dados: 52
Autores: Costa,Leonardo Severo da; Reiniger,Lia Rejane Silveira; Trindade,Helena; Heinzmann,Berta Maria; Bianchini,Nadia Helena.
Abstract Nectandra megapotamica (Spreng.) Mez. it is a native tree species of the Atlantic Forest, commonly known as canela-preta. The species has some anti-inflammatory, antitumor and antirheumatic properties among others. In this work the use of frozen plant material for microscopy analysis was tested. In addition, the leaf morpho-anatomy of the species was characterized which allowed to perform a structural description and to identify structures of secretion and storage of essential oil. The plant material was prepared for analyzes in optical, fluorescence and scanning electron microscopy. The leaf anatomy shows glabrous epidermis, unistratified, paracytic stomata, absence of trichomes, polyhedral epidermal cells. Some typical family characteristics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forest species; New methodology; Leaf anatomy; Essential oil; Secretory cavities.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100423

Bioactive compounds content of chimarrão infusions related to the moisture of yerba maté (Ilex Paraguariensis) leaves
Provedor de dados: 52
Autores: Bastos,Deborah H. M.; Fornari,Ana Claudia; Queiroz,Yara S.; Torres,Elizabeth A. F. S.
The aim of this study was to evaluate the effects of the processing stages of yerba maté (Ilex paraguariensis) on the moisture content of the leaves and the efficiency of the aqueous extraction of some bioactive substances. Samples of yerba maté were analyzed for caffeine, phenolic acids (caffeic acid, 5-caffeoilquinic acid) and flavonoids (quercetin, kaempferol and myricetin) by HPLC equipped with a diode array detector. Processing widely influenced the caffeine and 5-caffeoilquinic acid content of the aqueous extract (p &lt; 0.05), which was related to the moisture content of the leaves. Caffeic acid was present in 45% of the in infusions from dried mate leaves. Quercetin, myricetin and kaempferol were not detected.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba maté (Ilex paraguariensis); Processing; Bioactive substances.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400007

Plant cells in the context of climate change
Provedor de dados: 52
Autores: Machado,Marcelo Rubens.
Global warming and its origins triggered the beginning to considerable discussion in the last century. Studies of climate models presented in multidisciplinary scientific reports suggest that anthropogenic activities, particularly the emission of gases from the greenhouse effect, are greatly responsible for the current climate changes. The increase of carbon dioxide (CO2) atmospheric concentration has been in discussion in the news, scientific meetings and in public policy debates in several countries. Apart from its impact on global warming, the rising atmospheric CO2 has alerted the scientific community to the need to investigate any morpho-physiological alterations in the plants, given their direct influence on photosynthesis. This article aims to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbon dioxide; Global warming; Stomata; Climate change biology; Plant cell; Waxes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100018

N:Si:P atomic ratio in the Santa Cruz Channel, Itamaracá-PE (Northeast Brazil): a nyctemeral variation
Provedor de dados: 52
Autores: Montes,Manuel de Jesús Flores; Macêdo,Silvio José de; Koening,Maria Luise.
Studies were carried out on the Inlets of Orange and Catuama at the Santa Cruz Channel, Itamaracá-PE, in the rainy and dry seasons, in a nyctemeral cycle, and in a transversal profile, with intervals of three hours between collections. The objective was to determine the spacial and temporal variation of the N:Si:P atomic ratio, and its possible inter-relations and dependencies, as well as the influence of the hydrodynamism provoked by the variation of the tides at different times of the year. Nitrite-N and nitrate-N were higher in the rainy season, while ammonia-N, phosphate-P and silicate-Si were higher in the dry season, which indicated different results than previous studies. The N:P atomic ratio was lower than 16:1, in all seasons and stations, being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N:Si:P atomic ratio; Dissolved nutrients; Estuary; Nyctemeral variation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200002

Limnological patterns in northern pantanal lagoons
Provedor de dados: 52
Autores: Bleich,Monica Elisa; Silveira,Roberto Moraes Lima; Nogueira,Flávia Maria Barros.
The aim of this work was to correlate the limnological patterns recorded among ten lagoons in the northern part of the Pantanal of Mato Grosso with some aspects of the lagoons related to their physical and biological characteristics. Some lagoon characteristics, such as connection with the river and the presence of floating macrophytes were important to group similar lagoons into categories. Attempts were also made to determine which kind of the nutrients could be limiting phytoplankton productivity in the lagoons of the northern Pantanal. Data was collected in nine lagoons during the flooding stage in January 2004, and nutrient and chlorophyll a was followed in a tenth lagoon from June 2004 to October 2004. The phytoplankton biomass was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrient; Lagoon; Pantanal; Phytoplankton.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300028

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation
Provedor de dados: 52
Autores: Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002

Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
Provedor de dados: 52
Autores: Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz.
Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Organogenesis; Anatomy.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013

Separation of levan-formation and sucrose-hydrolysis catalyzed by levansucrase of Zymomonas mobilis using in vitro mutagenesis
Provedor de dados: 52
Autores: Sangiliyandi,G.; Kannan,T.R.; Raj,K. Chandra; Gunasekaran,P.
A levansucrase (SacB) of Zymomonas mobilis capable of sucrose hydrolysis but not levan formation was isolated through invitro mutagenesis of cloned sacB gene. When the sacB mutant gene was expressed in Escherichiacoli strains, only 50% of the sucrose-hydrolysing activity (2.0 U/mg) was produced, compared to the wild type levansucrase (4.0 U/mg). Sequencing of the sacB mutant gene revealed changes of two amino acid residues (Phe-102 to Leu and Trp-261 to Lys in the levansucrase). The absence of mutation at the site of Cys of SacB is contradictory to the inhibition kinetics that demonstrated the involvement of Cys in conferring the levan-forming activity to the SacB. The present finding is useful in understanding the mechanism of selective modulation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levansucrase; Polymerase; Sucrase; In vitro mutagenesis; Levan; Selective modulation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400001

Influence of some sugars on xylanase production by Aspergillus awamori in solid state fermentation
Provedor de dados: 52
Autores: Lemos,Judith Liliana Solórzano; Pereira Junior,Nei.
Aspergillus awamori showed high extracellular endoxylanase (100 U/ml) and beta-xylosidase activities (3.5 U/ml) when grown on milled sugar cane bagasse as the principal carbon source without treatment. Partial characterization of xylanases showed that the apparent values of Km were 3.12 ± 0.05 mg/ml for endoxylanase (in birchwood xylan) and 0.45 ± 0.05 mM for beta-xylosidase (in p -nitrophenyl beta-D-xylanopiranoside). Corresponding values of Vmax were 6.63 ± 0.02 and 0.078 ± 0.02 mumol/min. Gradual repression of endoxylanase activity was observed when increasing concentrations of glucose and xylose (1, 2, 4, 6 and 8 g of carbohydrate / 4 g of sugar cane bagasse) were added to production media. In contrast, beta-xylosidase activity was stimulated using low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus awamori; Endoxylanases; Beta-xylosidase; Catabolic repression.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600005

Evaluation of nutrient status and grain yield of two corn cultivars under different soil aluminum levels after liming
Provedor de dados: 52
Autores: Pauletti,Volnei; Prevedello,Beatriz Monte Serrat; Pissaia,Amir; Scopel,Airton.
This study was conducted to evaluate the effects of decreasing levels of Al saturation through the addition of lime under field conditions in the nutritional status and grain yield of two corn (Zea mays L.) cultivars: one tolerant and one non-tolerant to Al. The experiment was conducted on Red Yellow Latosol (Oxisol) at the Paraná, Brazil. Control plots received no lime (T0), the treatment T3 received lime to achieve 0% Al saturation and treatments T1 and T2 recieved 33.3% and 66.6%, respectively, of the lime applied to treatment T3. At full bloom stage, representative leaves ("nutritional leaves") were analysed for macro, micronutrients and Al contents, and in treatments T0 and T3 were also analyzed for Al distribution in shoot plants parts (stem, node,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum acid soil; Liming tolerant Zea mays corn.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300005

Bioenergetics Potential of RR2 PRO Transgenic Soybean Subjected to Application of Herbicides Isolated and in Combination
Provedor de dados: 52
Autores: Moreno,Giovane; Santos,Viviane Taisa; Albrecht,Leandro Paiola; Cesco,Victor Salomão.
ABSTRACT In Brazil, there are several crops producing oil for biofuel production, such as canola, sunflower, peanut, cotton, castor bean, soybean, among others. There are parameters that indicate the energetic and economic viability for the production of biofuels, and that can be applied, for example, in transgenic soybeans. The present study aimed to evaluate the energetic viability of Intacta RR2 PROTM soybean for biofuel production.Experiments were conducted in the municipalities of Palotina and Marechal Cândido Rondon, both in the State of Paraná. Energy balance calculations were carried out from the production system, estimating energy expenditure, including the agricultural and industrial stages. The inputs used were considered as input of energy,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Bioenergy; Biotechnology; Energy generation; Socioeconomic evaluation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200229

Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust
Provedor de dados: 52
Autores: Sing,Ngieng Ngui; Zulkharnain,Azham; Roslan,Hairul Azman; Assim,Zani; Husaini,Ahmad.
Four fungal isolates, SD12, SD14, SD19 and SD20 isolated from the aged sawdust grew on agar plates supplemented with PCP up to a concentration of 100 mg L-1. At high PCP concentration, isolate SD12 showed the highest radial growth rate of 10 mm day-1, followed by SD14 and SD19 both with 4.5 mm day-1 and SD20 with 4.2 mm day-1. Ultrastructural study on the effect of PCP on the PCP tolerant fungi using scanning electron microscope showed that high concentration of PCP caused the collapse of both fungal hyphae and spores. Among the four PCP tolerant fungi examined, isolate SD12 showed the least structural damage at high PCP concentration of 100 mg L-1. This fungal isolate was further characterized and identified as Cunninghamella sp. UMAS SD12. Preliminary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pentachlorophenol; Fungi; Cunninghamellasp.; Scanning Electron Microscopy; PCP degradation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600811

Fibromyalgia and the relevance of the whole-body vibration exercises in vibratory platforms: a short review
Provedor de dados: 52
Autores: Pinto,Nelson de Souza; Monteiro,Milena de Oliveira Bravo; Paiva,Dulciane Nunes; Santos-Filho,Sebastião David; Misssailidis,Sotiris; Thompson,Diane; Cabezuelo,Pedro Marín; Bernardo-Filho,Mario.
Among nonpharmacological strategy to manage fibromyalgia, exercise (aerobic) has shown efficacy. Whole-body vibration (WBV) exercise has been proposed as a potential clinical intervention. WBV would induce increase in growth hormone (GH). An impairment of the hypothalamic-pituitary-GH-Insulin Growth Factor-1(IGF-1) axis has been implicated in the pathophysiology of fibromyalgia. This article aims to review the studies on exploring the relationship between WBV and fibromyalgia. Literature searches were performed in the PubMed database on 04/03/2010 using terms related to "pain", "whole body vibration" and "fibromyalgia". An important number of publications were identified with the term "pain" and in comparison, only a small number of articles were found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibromyalgia; Whole body vibration; PubMed.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100007

Biotransformation of Benzaldehyde to L-Phenylacetylcarbinol (L-PAC) by Free Cells of Torulaspora delbrueckii in presence of Beta-Cyclodextrin
Provedor de dados: 52
Autores: Shukla,Vilas. B.; Kulkarni,Pushpa R.
Studies were carried out to explore the possibility of decreasing the toxic and inhibitory effects of the substrate benzaldehyde during its biotransformation to L-PAC by free cells of Torulaspora delbrueckii using beta -cyclodextrin (beta -CD). Use of various levels of benzaldehyde and acetaldehyde in presence of 2% of beta -CD showed that, in presence of beta -CD, the organism could tolerate higher levels of benzaldehyde and acetaldehyde. Semi-continuous feeding of benzaldehyde and acetaldehyde was found to increase the yield of L-PAC in comparison with one time feeding.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benzaldehyde; Biotransformation; Cyclodextrin; L-phenylacetycarbinol; Torulaspora delbrueckii.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300003

The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis
Provedor de dados: 52
Autores: Hajjari,Mohammadreza; Saffar,Behnaz.
We have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects on its efficiency. Eventually, we have been able to synthesize the gene in less than 40 minutes by Taq polymerase.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single step assembly PCR; Efficiency; Amplification primer; Polymerase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100007

Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines
Provedor de dados: 52
Autores: Shanmugam,Murugesan; Mahapatra,Ramkrishna.
Abstract The present study evaluated the effect of cryoprotectants, semen diluents and chicken lines during pellet method of semen cryopreservation. Three different experiments were conducted; Experiment 1 - semen was cryopreserved using dimethylformamide (DMF) at 6% and 9% concentrations in two semen diluents (Lake and Ravie diluent and TES/NaCl diluent), Experiment 2 - semen was cryopreserved using dimethylacetamide (DMA) at 6% and 9% with or without sucrose (100mM), Experiment 3- semen from two chicken lines (PD1 and PD6) was cryopreserved using DMA (6% and 9%). Semen was evaluated pre and post cryopreservation for progressive motility, live and abnormal sperm. Semen pellets were stored in cryovials for at least seven days before examination and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Diluent; Fertility; Pellet cryopreservation; Semen.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100501

Production and characterization of surface-active compounds from Gordonia amicalis
Provedor de dados: 52
Autores: Jackisch-Matsuura,Ani Beatriz; Santos,Leonardo Silva; Eberlin,Marcos Nogueira; Faria,Andréia Fonseca de; Matsuura,Takeshi; Grossman,Matthew James; Durrant,Lucia Regina.
Two methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium sulfate and produced predominantly water in oil emulsions (W/O). The crude Type I and II preparations were able to produce a significant reduction in the surface tension of water; however, the crude Type II preparation had 10-25 fold higher emulsification activity than the Type I preparation. Both SAC preparations were analyzed by the TLC and each produced two distinct bands with Rf 0.44 and 0.62 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioemulsifier; Bioremediation; Biosurfactant; Gordonia amicalis; Industrial chemicals; Microbial surfactant production.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100019

Germination of Jacaranda mimosifolia (D. Don - Bignoniaceae) seeds: effects of light, temperature and water stress
Provedor de dados: 52
Autores: Socolowski,Fábio; Takaki,Massanori.
Investigations were carried out to study the effect of light, temperature and water stress on the germination of seeds of Jacaranda mimosifolia which showed the minimum and maximum germination temperature at 15 and 40º C, respectively. The optimum temperature was 25º C with high percentage and germination rate. Slight promotive effect of white light was observed. Under water stress conditions the effect of light was high but at optimum temperature no effect of light was observed. At -0.9MPa few seeds germinated.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jacaranda mimosifolia; Seed germination; Phytochrome; Water stress; Light sensitivity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500014

Uso da taxocenose de peixes como indicadora de degradação ambiental no rio Paraíba do Sul, Rio de Janeiro, Brasil
Provedor de dados: 52
Autores: Araújo,Francisco Gerson.
An adaptation of index of biotic integrity (IBI) was performed for the first time in Brazil at the Rio Paraíba do Sul, between Barra Mansa and Barra do Piraí, a very important extent of this river because of the high pollution levels, and for being the main abstraction source of Rio de Janeiro city water supply. A monthly standardised sampling programme, from March-1995 to February-1996, was carried out at four sites. Seines, casting nets and net trays were used for catching fishes. IBI was obtained by integrating 12 ecological and environmental attributes of fish taxocene, populations, and individual organisms to assess biological integrity based in Species Composition, Trophic Structure and Fish Condition. Overall, water quality was classified between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rio Paraíba do Sul; Integridade Biótica; Peixes; Poluição.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300016

Enhanced immune response of a bicistronic DNA vaccine expressing fusion antigen Hsp65-Esat-6 of Mycobacterium Tuberculosis with GM-CSF as a molecular adjuvant
Provedor de dados: 52
Autores: Dong,Yan; Gong,Jun-Yuan; Liu,Xin; Li,Jun-Wu.
This study aimed to construct a bicistronic DNA vaccine expressing fusion antigen Hsp65-Esat-6 of Mycobacterium tuberculosis with cytokine GM-CSF as a molecular adjuvant (pIRES-Hsp65-ESAT-6-GM-CSF, pIRHEG), and the immune response in mice. C57BL/6 mice were immunized with the recombinant plasmid to detect the titer of antibodies, lymphocyte proliferation, the ratio of CD4+, CD8+T cell and IFN ~ γï¼ŒIL-2 secretion. The titer of antibody, lymphocyte proliferation, the ratio of CD4+T and CD8+T cells and IFN ~ γ, IL-2 secretion of pIRHEG group was significant higher than other recombinant plasmid groups, which significant differed by statistical mean. The bicistronic DNA vaccine could induce an effective immune response in mice and could be used as vital...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hsp65; Esat6; GM-CSF.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500006

Short-term leaf responses of Nicotiana tabacum 'Bel-W3' to ozone under the environmental conditions of São Paulo, SE - Brazil
Provedor de dados: 52
Autores: Souza,Silvia Ribeiro de; Sant'Anna,Silvia Maria Romano; Rinaldi,Mirian Cilene Spasiani; Domingos,Marisa.
The present study aimed to study if the intensity and precocity of short-term leaf responses of Nicotiana tabacum 'Bel-W3' depended on the daily atmospheric levels of ozone and/or daily meteorological conditions in a State Park located in the city of São Paulo, SE Brazil. From 16 September to 14 October 2002, three lots of six potted plants were exposed for fourteen days. Leaf necrosis, atmospheric ozone concentrations and meteorological parameters were daily measured. The average percentage of leaf necrosis ranged from 5% to 20%. A 4th degree polynomial model was proposed to explain daily progress of leaf necrosis on plants. The average ozone levels were increased from morning (1.3-39.2 ppbv) to the midday (1.6-52.0 ppbv) and decreased in the evening...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf necrosis; N. tabacum Bel-W3; Ozone; Meteorological parameters.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100030

Comparison between multivariate methods applied for the evaluation of genetic divergence in Cacao (Theobroma cacao L.)
Provedor de dados: 52
Autores: Dias,Luiz Antônio dos Santos; Kageyama,Paulo Yoshio.
Several multivariate methods have been used in divergence analyses of populations. Consistency and relative association among four methods were assessed using a 5 x 5 complete-diallel data involving cacao cultivars. Over a 5-year period, five cultivars were analyzed based upon five yield components. In assessing the divergence of parents only the data obtained from five cacao cultivars were analyzed. Four multivariate statistics presented close association when considered in pairs, in this case the Mahalanobis' (D²) with the mean Euclidean distance obtained from canonical variates (d cv), and mean Euclidean distance (d e) with the mean Euclidean distance obtained from principal components (d pc). In both cases, high correlations (r &gt; 0.95) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cacao cultivars; Canonical variates; Genetic divergence; Mahalanobis' and mean Euclidean distances; Principal components.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200005

Effects of Emerita brasiliensis flour supplementation on normotensive (Wistar) and spontaneously hypertensive rats (SHR)
Provedor de dados: 52
Autores: Cavargere,Victor Agati; França,Camille Feitoza; Cardoso,Ricardo; Vianna,Lucia Marques.
Several studies characterize the connection of overweight and obesity with chronic diseases. Therefore, new alternatives are being studied for controlling hypertension, such as chitin and chitosan fibers, commonly found on crustacean's carapace like Emerita brasiliensis. Rats from two different strains were divided into control and supplemented groups (n=6). The Wistar strain experiment started with a 14-days baseline period, followed by supplementation of E.brasiliensis flour added to the diet in the doses of 5, 10 and 20%, for a 14-days period each. With the optimal dose of 20%, the study was undertaken with SHR rats, starting with a 7-days baseline period, followed by three weeks of supplementation. Data were evaluated using one-way ANOVA and p&lt;0.05...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitin; Chitosan; Emerita brasiliensis; Blood pressure.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400006

Rosemary and Pitanga Aqueous Leaf Extracts On Beef Patties Stability under Cold Storage
Provedor de dados: 52
Autores: Vargas,Flávia Carolina; Arantes-Pereira,Lucas; Costa,Patrícia Argemira da; Melo,Mariza Pires de; Sobral,Paulo José do Amaral.
ABSTRACT Because processing and storage conditions affect several beef quality attributes, the food industry uses a variety of synthetic antioxidants. However, some synthetic antioxidants have been questioned regarding its safety, and thus the interest in using natural antioxidants in food products is increasing. This paper aimed at assessing leaf aqueous extracts of Rosemary (Rosmarinus officinalis Linnaeus) and Pitanga (Eugenia uniflora Linnaeus) as antioxidants in beef cold storage. After 48h storage, patties added of Rosemary leaf extracts showed increased pH. Patties added of Pitanga extracts had the lowest a* color values. Oxymyoglobin levels were significantly higher for Negative control, than for Pitanga treatment. The 10% extract addition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Myoglobin; Color; Oxidation; Lipid.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100514

The vascular flora of the cerrado in Emas National Park (Central Brazil): a savanna flora summarized
Provedor de dados: 52
Autores: Batalha,Marco Antônio; Martins,Fernando Roberto.
The Brazilian cerrado may be divided in two main phytogeographic sectors: one characterized by Piptocarpha rotundifolia, in which the Emas National Park (ENP) is located, and other characterized by Curatella americana. We carried out a floristic survey in ENP, which allowed an assessment of the taxonomic composition, taxa size, and similarity with other sites for the ENP's vascular flora. We compared the ENP's flora with southeastern outlying cerrado sites, also in the Piptocarpha sector, and with general floristic patterns in the cerrado vegetation. The distribution of species per family in ENP was significantly different from that obtained for each component of the general cerrado flora. The herbaceous component was characterized by an overproportion of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Floristics; Phytogeography; Principal component analysis; Savanna.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200012

Evaluation of carcass characteristics and meat chemical composition of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in pasture systems
Provedor de dados: 52
Autores: Moreira,Fernanda Barros; Souza,Nilson Evelázio de; Matsushita,Makoto; Prado,Ivanor Nunes do; Nascimento,Willian Gonçalves do.
The objective of this study was to evaluate the carcass characteristics (carcass weight, carcass yield, fat thickness, loin area, marbling and colour) and chemical composition of the Longissimus dorsi muscle (moisture, ash, crude protein, fat and cholesterol) of cuts with or without fat thickness, of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in millet (Pennisetum americanum L.) or star grass (Cynodon plectostachyus Pilger) pasture systems, with mineral or mineral protein supplementation. Animals were slaughtered with an average body weight of 450 kg (Bos indicus) or 470 kg (Bos indicus x Bos taurus crossbreed). There was no treatments effect on carcass characteristics and meat chemical composition of cut without fat thickness. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; Bos taurus; Carcass; Meat; Pasture.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400016

Caracterização sedimentológica da planície intertidal da parte sul do saco do Limoeiro (Ilha do Mel - Paraná - Brasil): I. implicações ecológicas
Provedor de dados: 52
Autores: Couto,Erminda da Conceição Guerreiro; Savian,Michelle.
Sediment cores 40 cm depth were taken in triplicate at nine points along an unvegetable intertidal flat, to assess the ecological implications of sedimentological characteristics. Each core was sectioned in 5 cm depht layers. In these sub-samples temperature, pH, water, organic matter and carbonate content were determined. Granulometric analysis, porosity, qualitative and quantitative identification of sediment components were also carried. The sediments presented low water (15.6 - 37.2 %), organic matter (0.7 - 5.0 %) and carbonates (0.3 - 1.0 %) contents, prevailing fine to very fine sand at surface. Statistical analysis showed a strong horizontal homogeneity regarding sedimentological and physiochemical parameters. Organic matter exhibit a general trend...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Planície intertidal; Sedimento; Análise granulométrica; Calcáreo biodetrítico.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200011

Gas exchange and plant growth after defoliation on Leandra lacunosa, a cerrado woody species with continuous leaf production
Provedor de dados: 52
Autores: Damascos,Maria Angélica; Ronquim,Carlos Cesar; Prado,Carlos Henrique Britto Assis.
Morphological and physiological leaf traits were studied on control and defoliated potted Leandra lacunosa young plants. After defoliation, remaining bottom leaves showed no significant change in net photosynthesis and stomatal conductance values with time, but the leaf transpiration rate was higher. The production of the new leaves was greater after defoliation in top defoliated plants. Bottom defoliated plants increased stem extension rates and net photosynthesis transitorily. Two months after defoliation, control and defoliated plants did not differ in stem length and root dry mass values. In spite of smaller area and photosynthetic capacity, remaining bottom leaves were able to compensate top leaves elimination. The effectiveness of this compensatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compensatory photosynthesis; Herbivory; Leaf age; Leaf phenology; Leandra lacunosa.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800013

Solexa Profiling Identifies Differentially Expressed MiRNAs Between Sexually Immature and Mature Equine Testis
Provedor de dados: 52
Autores: He,Liangjun; Wang,Shiwei; Deng,Haifeng; Dong,Hong; Chen,Jingbo.
ABSTRACT MicroRNAs (miRNAs) are a class of short non-coding RNAs identified as potent regulators of gene expression. Previous studies have suggested that miRNAs are involved in mammalian spermatogenesis. Stallion fertility is an important trait for the horse breeding industry, but stallion fertility traits are largely ignored in the industry. In this study, we generated expression profiles of miRNAs in foal (immature) and stallion (mature) testes using Solexa sequencing. We identified 438 known and homologous equine miRNAs and 199 novel miRNAs which were distributed among all the chromosomes. The two developmental stages showed significant differences in miRNA expression patterns. Our result expands the horse miRNA database and provided additional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; MiRNAs; Solexa; Testis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100307

Apoptosis and Histopathology of the Heart after Renal Ischemia-Reperfusion in Male Rat Running title: Ischemia-Reperfusion Injury
Provedor de dados: 52
Autores: Alihemmati,Alireza; Yousefi,Hadi; Ahmadiasl,Naser; Habibi,Parisa.
ABSTRACT Ischemia-reperfusion injury was seen in strokes, myocardial infarctions, acute kidney injury, mesenteric ischemia, liver and systemic shock. Renal ischemia-reperfusion is more importance in the setting of kidney transplantation that affects distant organs. In this study forty Male Albino Wistar rats (200-250g) were randomly divided in four group (n=10) including control, sham operation group, nephrectomy and IRI group. All rats anesthetized with intraperitoneal injection of ketamine (50 mg/kg) and xylazine (10 mg/kg) and maintained the core body temperature at approximately 37°C. For inducing IRI group, it was performed right nephrectomy, and in continuing, the left kidney pedicle occluded to 45 min via nontraumatic microvascular clamp for making...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal Ischemia-reperfusion injury; Glycogen accumulation; Apoptosis; Heart; Rat.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100443

An adapted thermal-gradient block for the germination of photoblastic seeds
Provedor de dados: 52
Autores: Cardoso,Victor José Mendes.
This work describes an adapted thermal-gradient block with internal illumination, allowing experiments with light-requiring seeds. The temperature dependence study on the germination of the photoblastic Plantago tomentosa seeds was carried out in order to test the performance of the apparatus. With the aid of the model described here, some parameters related to the response of the seeds to temperature could be estimated, showing that the apparatus was suitable for the studies on the temperature dependence of photoblastic seeds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temperature devices; Thermal-biology; Plantago tomentosa.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600002

Relationships among sensory analysis, isoflavone and hexanal contents of soymilk powder
Provedor de dados: 52
Autores: Silva,Josemeyre Bonifácio da; Felberg,Ilana; Carrão-Panizzi,Mercedes Concórdia; Lee,Soo Young; Prudencio,Sandra Helena.
The objective of this work was to study the sensory attributes and their relationships with isoflavones and hexanal contents of soymilks made in laboratory and commercial samples. The laboratory soymilk samples showed cooked grain and cotton candy aroma and cooked grain, malty and sweetness flavor (a mild flavor). The commercial samples presented stronger roasted soy, rancid, sesame seeds and fishy aroma and roasted soy, sesame seeds and bitterness flavor, and bitter taste (closed nose) and starchy texture. No differences were noted among laboratory soymilks, denoting inactivation of lipoxygenases enzymes in the soymilks process. There were differences between the samples prepared in laboratory and commercial soymilks, which was due to several factors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quantitative descriptive analysis; Hexanal; Lipoxygenases; Vegetable type soybean; Isoflavones.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500025

Production of 2,3-Butanediol from Sucrose by Klebsiella pneumoniae NRRL B199 in Batch and Fed-Batch Reactors
Provedor de dados: 52
Autores: Silveira,Mauricio M.; Berbert-Molina,M.; Prata,A. M. R.; Schmidell,W.
In batch mode, Klebsiella pneumoniae growth and 2,3-butanediol/acetoin formation are increasingly inhibited by initial sucrose concentrations (S0) over 60 g/L. At non inhibitory conditions, a maximum sucrose consumption rate of 1,5 g/L/h was measured. With S0=204 g/L however, this rate decreased to 0.15 g/L/h. K. pneumoniae fermented 204 g/L sucrose to produce 84.3 g/L of a mixture 2,3-butanediol/acetoin with a yield of 0.41 g/g and a productivity of 1.06 g/L/h. Higher oxygen transfer rates improved the overall process rate but the product yield was reduced. Avoiding substrate inhibition, by performing the fermentation in fed-batch mode, a final 2,3-butanediol/acetoin concentration of 80.0 g/L was achieved. In this case, a productivity of 2.63 g/L/h and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae 2; 3-butanediol/batch process fed-batch process.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300009

Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview
Provedor de dados: 52
Autores: Fathi,Ezzatollah; Farahzadi,Raheleh.
ABSTRACT The aim of this review was to describe the current state-of-the-art regarding isolation, characterization and aging of adipose tissue-derived mesenchymal stem cells (ADSCs). Mesenchymal stem cells (MSCs) have recently received widespread attention because of their potential use in tissue-engineering applications. Various studies have indicated that MSCs with a fibroblast-like morphology migrate to the sites of injury and help to regenerate damaged tissue. Over the past few years, it has been recognized that fat is not only an energy supply, but also a rich source of multipotent stem cells that can be easily harvested, isolated and selected as compared with other tissues. ADSCs are particularly interesting because of their rapid proliferation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue-derived mesenchymal stem cells; Adipose tissue; Isolation; Aging; Differentiation potential.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100302

Soil resistance to penetration and least limiting water range for soybean yield in a haplustox from Brazil
Provedor de dados: 52
Autores: Beutler,Amauri Nelson; Centurion,José Frederico; Silva,Alvaro Pires da.
The objective of this study was determine the resistance to penetration (PR), least limiting water range (LLWR) and critical bulk density (Db-crit) for soybean yield in a medium-textured oxisol (Haplustox). The treatments represented the soil compaction by passing a tractor over the site 0, 1, 2, 4, and 6 times, with 4 replications in a randomized experimental design. Samples were collected from 0.02-0.05, 0.07-0.10 and 0.15-0.18 m depths. Soybean (Glycine max cv. Embrapa 48) was sowed in December 2002. Plant height, number of pods, aerial dry matter, weight of 100 seeds, and the yield in 3.6 m² plots were recorded. Soybean yield started reduction at the PR of 0.85 MPa and Db of 1.48 Mg m-3. The LLWR was limited in highest part by water content at field...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Water content; Resistance to penetration; Bulk density.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800002

Floristic and phytosociological description of a riparian forest and the relationship with the edaphic environment in Caiuá Ecological Station - Paraná - Brazil
Provedor de dados: 52
Autores: Costa Filho,Lysias Vellozo da; Nanni,Marcos Rafael; Campos,João Batista.
Relationship in soil physical and chemical properties of soil, floristic and phytosociological association of semi-deciduous riparian forest of the Paranapanema River (R) and Rosana Reservoir (Re) in the Caiuá Ecological Station were evaluated. Aerial photography and satellite image were used to determine the forest cover and to locate 15 transects 50 x 30m (1500m²),which were used to sample trees with diameters at breast height (DBH)&gt;15 cm. R contained 1487 individuals from 33 families, 64 genders and 73 species. The Shannon-weaver index (H') was 3.318. Re contained 1146 individuals from 35 families, 72 genders and 85 species and the H' was 3.755. There was a statistically significant difference (P&lt;0.05) between R and Re environments and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical semi deciduous forest; Riverine forest; Multivariate analysis; Riparian forest; Soil-vegetation; Caiuá Ecological Station.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600013

Extraction of Indigo from Some Isatis species and Dyeing Standardization Using Low-technology Methods
Provedor de dados: 52
Autores: Comlekcioglu,Nazan; Efe,Lale; Karaman,Sengul.
Fresh leaves of four Isatis species culture form of I. tinctoria L and wild forms of I. buschiana Schischkin, I. candolleana Boiss. (endemic) and I. tinctoria L. subsp. corymbosa. (Boiss.) were used for indigo production. Dyes were extracted by fermentation and hot water application. The extracted dyes were optimized with different pH and reducing agents. Results showed that the dye from hot water application produced the desired dying quality at pH 11. Reducing agent concentrations had no significant effect on color quality. Dark blue and blue colors were obtained from I. tinctoria and I. candolleana extracts although I. tinctoria subsp. corymbosa and I. buschiana produced mostly yellow-gray colors. Light, dry and wet rubbing fastness values varied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isatisspp.; Woad; Indigo; Natural dye; Fermentation; Fastness; Spectrophotometry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100096

The clinical impact of [18F]-FDG Pet during the opening year of a Pet centre
Provedor de dados: 52
Autores: Talbot,Jean-Noël.
We have evaluated the clinical impact of FDG-PET on patient staging and management during the opening year of our PET centre in France. A questionnaire, translation in French of the questionnaire used recently in California, was sent to the referring physician of each of the 476 patients who had at least one routine FDG-PET examination during the year 2000. Of 348 responses (response rate = 73%), the disease was upstaged in 26% of the cases and downstaged in 9%. Inter-modality management changes (change from a scheduled therapeutic modality for a different one) were reported in 37% of the cases and intra-modality changes in 9%. Those modification rates were respectively 38% and 7% in recurrence of colorectal cancer (153 patients), 47% and 7% in lung cancer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PET; [18F]-FDG; Clinical impact; Cancer.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500009

Damage assessment of the equine sperm membranes by fluorimetric technique
Provedor de dados: 52
Autores: Celeghini,Eneiva Carla Carvalho; Andrade,André Furugen Cesar de; Raphael,Cláudia Fernandes; Nascimento,Juliana; Ticianelli,Janahi Souza; Arruda,Rubens Paes de.
To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stallion; Acrosome; Plasma membrane; Mitochondria; Fluorescent probes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004

Effect of lactic acid and UV irradiation on the cassava and corn starches
Provedor de dados: 52
Autores: Franco,Célia Maria Landi; Ogawa,Cristina; Rabachini,Taís; Rocha,Thais de Souza; Cereda,Marney Pascoli; Jane,Jay-lin.
In this work, the effect of lactic acid and UV irradiation on the physicochemical and structural characteristics of cassava and corn starches was evaluated. Only the modified cassava starch presented baking expansion capacity. From RVA, reduction of viscosity values, greater internal stability and none set back for modified cassava starch were observed. Modified corn starch did not show any peak viscosity. There were no significant differences in DSC thermal properties of treated and native starches. Amylopectin and amylose molecules from both the modified starches displayed some degradation. Molecular weight of cassava amylopectin was mostly preserved, whereas corn amylopectin was evenly attacked through the granule. Nevertheless, the B long branched...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Starch; Photochemical treatment; Expansion capacity; Structural and physicochemical characteristics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200025

Crystal morphology of binary and ternary mixtures of hydrogenated fats and soybean oil
Provedor de dados: 52
Autores: Simões,Ilka Sumiyoshi; Gioielli,Luiz Antonio.
The objective of this study was to verify the influence of temperature on crystallization of binary and ternary mixtures of two hydrogenated fats and soybean oil, by polarized light microscopy at temperatures of 30° C, 35° C, and 40° C. The types of crystals observed were spherulites type A, and B and the polymorphic forms were ß , and ß -prime. The soybean oil does not contribute statistically to total area or maximum diameter of the crystals. At 35° C the positive relative coefficients to the interactions presented, in general, absolute values higher than the negative ones, pointing that the crystals were larger than what could be expected, if there was no interaction among the components. At 40° C the negative relative coefficients revealed, in general,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crystal morphology; Hydrogenated fat; Mixture; X-ray diffraction.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200015

Cladia aggregata (lichen) from Brazilian northeast: chemical characterization and antimicrobial activity
Provedor de dados: 52
Autores: Martins,Mônica Cristina Barroso; Lima,Marcio James Gonçalves de; Silva,Flávia Pereira; Azevedo-Ximenes,Eulália; Silva,Nicácio Henrique da; Pereira,Eugênia Cristina.
The chemical composition and antimicrobial activity of the Cladia aggregata (Sw.) Nyl. were evaluated. Barbatic acid, depside obtained from C. aggregata, was spectroscopically analyzed and tested, as well as organic extracts. The extracts and purified substance were tested against four Staphylococcus aureus multi-resistant strains. The structure of barbatic acid was confirmed through NMR (¹H; 13C) and elemental ® analysis. Biochromatographic assays showed action of this compound, along with other substances contained in organic extracts, suggesting a synergic action, MIC assays placed barbatic acid in the same level of inhibition to other studied lichen substances.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cladia aggregata; Barbatic acid; Antimicrobial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100015

Copper improves the production of laccase by the white-rot fungus Pleurotus pulmonarius in solid state fermentation
Provedor de dados: 52
Autores: Tychanowicz,Giovana Kirst; Souza,Daniela F. de; Souza,Cristina G. M.; Kadowaki,Marina Kimiko; Peralta,Rosane Marina.
Pleurotus pulmonarius (Fr) Quélet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copper; Laccase; Pleurotus pulmonarius; Phenoloxidase; Solid state fermentation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600002

Effect of gamma radiation on lipids by the TBARS and NMR
Provedor de dados: 52
Autores: Silva,Adriana Cristina de Oliveira; Mársico,Eliane Teixeira; Jesus,Edgar Francisco Oliveira de; Guimarães,Carlos Frederico; Cortez,Marco Antonio Sloboda.
The aim of this work was to study the effect of gamma radiation on lipids by TBARS and NMR. The samples of raw whole milk were subjected to gamma radiation from Co60 in doses of 1, 2 and 3 kGy and the production of rancidity was studied through Nuclear Magnetic Resonance (NMR) and Thiobarbituric Acid Test (2-TBARS). The TBARS values increased according to the intensity of the radiation dose applied at the samples, demonstrating correlation between the radiation dose and the production of lipid oxidation. This was confirmed by NMR with the formation of peaks of aldehydes and ketones that were small and similar in the doses of 1 and 2 kGy. In the dose of 3 kGy, the total degradation of milk fat was observed. A correlation between the NMR and 2-TBA was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk; Gamma radiation; Rancidity; Thiobarbituric Acid Test; Nuclear Magnetic Resonance (NMR).
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600021

Studies on fungal and bacterial population of air-conditioned environments
Provedor de dados: 52
Autores: Ross,Claudia; Menezes,Jose Roberto de; Svidzinski,Terezinha Inez Estivalet; Albino,Ulisses; Andrade,Galdino.
In tropical countries such as Brazil, there is not enough information about microbial contaminants in indoor environments with air conditioning systems. Microbial monitoring of such environments is important for the quality of human life. The aim of this work was to assess the fungal genera and bacterial morphotypes occurring in such environments. Air samples were taken indoors and outdoors from a public auditorium, a hospital, a company and a shopping center during the 2001 winter by using a six-stage impactor Millipore M air T® . Twenty-one fungal genera were identified. Bacterial morphological groups found were Gram positive and negative rods and Gram positive coccus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sick building syndrome; Fungi; Bacteria; Air conditioner; Indoor air quality; Air ecology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500020

Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
Provedor de dados: 52
Autores: Portes,Ciro da Silva; Oliveira,Andriélen Virke de; Simer,Patrícia; Lunkes,Alessandra Machado; Coelho,Alexandre Rodrigo.
This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moulds; Killer yeasts; Biocontrol; Antibiosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400012

Micropropagation and in vitro conservation of Neoglaziovia variegata (Arr. Cam.) mez, a fiber producing bromeliad from Brazil
Provedor de dados: 52
Autores: Silveira,Daniela Garcia; Souza,Fernanda Vidigal Duarte; Pelacani,Claudinéia Regina; Souza,Antonio da Silva; Ledo,Carlos Alberto da Silva; Santana,José Raniere Ferreira de.
Neoglaziovia variegata (Arr. Cam.) Mez is a Bromeliaceae native to the Caatinga, used for fiber extraction in the Northeast Region of Brazil. The antropic activity has place this species among the threatened ones. The objective of the work was to establish an in vitro propagation and conservation of caroá. Seeds were cultivated in MS medium in the presence or absence of light. In vitro germinated seedlings were multiplied in MS medium supplemented with the combinations 0.05 and 0.50 µM NAA and 2.2 and 4.4 µM BAP and KIN. The best percentages of germination were obtained with the seeds incubated in the presence of light. The highest multiplication ratio was obtained for the NAA (0,5 µM) + BAP (4,4 µM) treatment and the number of roots, with NAA (0.5 µM) +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acclimatization; Bromeliaceae; Fiber extraction; In vitro preservation; Seedling production.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400016

Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes
Provedor de dados: 52
Autores: Alves,Andrea Catão; Gontijo,Cristiano Machado; Oliveira,Mônica Cristina; Diniz,Simone Odília Fernandes; Oliveira,Flávia Márcia; Cardoso,Valbert Nascimento; Ramaldes,Gilson Andrade.
The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liposomes; Oral absorption; Mucosal delivery; Proteins; Nanotechnology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700035

Germination in seed species ingested by opossums: implications for seed dispersal and forest conservation
Provedor de dados: 52
Autores: Cáceres,Nilton Carlos; Monteiro-Filho,Emygdio Leite de Araújo.
Seed germination in plant species consumed by opossums, genus Didelphis, was investigated in southern Brazil, in order to improve knowledge of the strategies of zoochorous plants in the Neotropics. Seeds were obtained from opossum feces. Thirteen of the most frequent species in the diet of local opossums were tested for germination rates and germination responses under different qualities (red/far red ratio) and different intensities of light. Most seeds from feces germinated similarly to the control groups, except for seeds of Rubus rosifolius, which appeared to depend on gut passage. Other experiments revealed that most seeds in the opossums' diet were of pioneer species, with most germination occurring during favorable humid conditions in the rainy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seed germination; Light quality; Dormancy; Marsupials.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700002

Morphological patterns of fish and their relationships with reservoirs hydrodynamics
Provedor de dados: 52
Autores: Cunico,Almir Manoel; Agostinho,Angelo Antonio.
The aim of this study was to determine morphological patterns of the most abundant fish species found in reservoirs with different hydraulic retention times and stages of colonization, considering habitat strata and feeding strategies. Samplings were carried out in six Paraná State (Brazil) reservoirs, and morphological data were obtained from 15 individuals of 17 fish species. Ecomorphological attributes that best segregated species, were selected using Discriminant Analysis. These selected attributes were later used in a Principal Components Analysis (PCA). Differences among reservoirs concerning occupation of the ecomorphological space were tested through analysis of variance (null models). The six reservoirs differed in the occupation of the fish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecomorphology; Feeding strategies; Habitat; Reservoir.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100015

Osmo-dehydrated functional product containing fructo-oligosaccharides: physical, chemical and sensorial characteristics
Provedor de dados: 52
Autores: Egea,Mariana Buranelo; Borsato,Dionísio; Silva,Rui Sérgio dos Santos Ferreira da; Yamashita,Fabio.
The objective of the present study was to develop a functional product from the osmo-dehydrated Fuji apple incorporating FOS with acceptable physical, chemical and sensorial characteristics and potential for commercial-scale production. Some treatments were discarded due to texture and water activity that did not reach the desired quality parameters. The products submitted to the osmo-dehydration treatments presented a greater vitamin C loss. All the treatments obtained sensory scores of 6 in a 9 points scale for all the attributes, except the crispness, indicating good acceptance. The sensory scores and incorporated FOS quantities indicated that the osmo-dehydrated apple products have potential for commercial-scale production.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sensory analysis; Functional product; Apple; Combined conservation methods.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600017

Morphometric and ultrastructural studies of the effect of infrared laser on rabbit temporo-mandibular joint fibroblasts
Provedor de dados: 52
Autores: Uribe,Ricardo Cornejo; Vargas,Fernando Matamala; Mella,Héctor Silva; Onate,Orlando Garrido.
The infrared laser beams has been successfully used in joint lesions treatment due to its analgesic anti inflamatory and healing action, and on a cellular level, inducing an accentuated synthesis of ATP, activating cellular proliferation and collagen synthesis. Samples of normal-control and irradiated tissue from temporo-mandibular joint disc of rabbit, were obtained, with daily doses of 2 joules/cm²during 10 consecutive days. They were prepared for transmission electronic microscopy.Transmission electronic micrographs of normal and irradiated fibroblasts were obtained with final increase of 11.500 X for morphometric studies with the purpose of quantifying the volumetric fractions of the cellular components pertaining to the mentioned cellular types....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblasts; Laser; Morphometry.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300013

Evaluation of the types of starch for preparation of LDPE/starch blends
Provedor de dados: 52
Autores: Vinhas,Glória Maria; Lima,Suzana Moreira de; Santos,Lívia Almeida; Lima,Maria Alice Gomes de Andrade; Almeida,Yêda Medeiros Bastos de.
This study evaluated in relation the growth, and the amylolytic activity of mixed and isolated cultures of Phanerochaete chrysosporium and Talaromyces wortmanni on different types of starch. The thermal and mechanical properties in polyethylene/starch blends (proportion: 80/20 (w/w) before and after inoculation of the mixed cultures were evaluated. The regular starch Amidex 3 and the modified starch Fox5901 stood out in relation to the cellular growth and production of the amylase enzyme. In spite of the short time that the blends were exposed to the fungi, the microorganisms promoted physical and chemical changes in the structure of the blend, modifying its thermal and mechanical properties. The alteration of the degree of crystallinity and mechanical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylolytic enzymes; Polyethylene; Starch; Blends; Talaromyces wortmanii; Phanerochaete crysosporium.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300001

Physiologic sexual maturity of the fiddler crab Uca rapax (Smith, 1870) (Crustacea, Ocypodidae) from two mangroves in Ubatuba, Brazil
Provedor de dados: 52
Autores: Castiglioni,Daniela da Silva; Negreiros-Fransozo,Maria Lucia.
The gonad development of Uca rapax was studied to achieve the size at onset of its sexual maturity. Crabs were sampled from April/2001 to March/2002 in the Itamambuca and Ubatumirim mangroves in Ubatuba, São Paulo, Brazil. The specimens were grouped in 10 size classes. Juveniles and adult crabs frequencies were assessed for each class. The size of carapace width in which 50% of males and females were considered mature was 14.8 and 12.1 mm (Itamambuca) and 13.6 and 11.4 mm (Ubatumirim), respectively for males and females. Males matured at higher sizes than females, probably due to a major investment in their somatic growth, while females spend their energy in the reproductive process, saving energy for eggs' production.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maturity; Uca rapax; Ocypodidae; Mangroves.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300009

Erratum: Phenolic Content and Biomolecule Oxidation Protective Activity of Globularia alypum Extracts
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100905

Variation on the amount of winter cover crops residues on weeds incidence and soil seed bank during an agricultural year
Provedor de dados: 52
Autores: Mauli,Márcia Maria; Nóbrega,Lúcia Helena Pereira; Rosa,Danielle Medina; Lima,Gislaine Piccolo de; Ralish,Ricardo.
This study analyzed possible interferences associated to the amount of crop residues produced by the black oats and the consortium of black oats, common vetch and forage turnip on weeds incidence and soil seed bank. It was a field trial with seven treatments and five replications. The cover crop was sown at throwing, cut at 100 days and residues were put on each respective plot, using a proportion of normal amount of produced straw, either its half and double. The heaviest weights were obtained from cover crop consortium and their application decreased weeds incidence in such area. The seeds bank and other analyzed parameters did not show statistical differences. According to these results, it was concluded that winter cover crop could be used in crops...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: No-tillage system; Seeds bank; Weeds incidence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400006

Effect of feeding methods on the astaxanthin production by Phaffia rhodozyma in fed-batch process
Provedor de dados: 52
Autores: Moriel,Danilo Gomes; Chociai,Miriam Blumel; Machado,Iara Maria Pereira; Fontana,José Domingos; Bonfim,Tania Maria Bordin.
The effect of feeding methods on the production of astaxanthin by the yeast Phaffia rhodozyma ATCC 24202 was studied, using continuous and pulsed fed-batch processes and low cost materials as substrates (sugar cane juice and urea). In continuous fed-batch processes, a cellular astaxanthin concentration of 383.73 µg/g biomass was obtained. But in pulsed fed-batch processes a reduction in the cellular astaxanthin concentration (303.34 µg/g biomass) was observed. Thus the continuous fed-batch processes could be an alternative to industrial production of astaxanthin, allowing an increase in the biomass productivity without losses on astaxanthin production by the yeast.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astaxanthin; Fed-batch process; Phaffia rhodozyma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300010

Effect of naringerin on biochemical parameters in the streptozotocin-induced diabetic rats
Provedor de dados: 52
Autores: Fernandes,Ana Angélica Henrique; Novelli,Ethel Lourenzi Barbosa; Fernandes Junior,Ary; Galhardi,Cristiano Machado.
Amongst the numerous co-adjuvant therapies which could influence the incidence and progression of diabetic complications, antioxidants and flavonoids are currently being tested in clinical trials. We investigated the effect of naringerin on biochemical parameters in streptozotocin-induced (STZ - 60 mg/kg, i.p.) diabetic rats. Male rats were divided into four groups: G1: untreated controls; G2: normal rats receiving naringerin; G3: untreated diabetics; G4: diabetics rats receiving naringerin. The naringerin (50mg/kg, i.p,) decreased the hyperglycaemia and hyperlipidaemia associated with STZ-diabetes. The concentrations of serum insulin in treated diabetic rats tended to be increased. Naringerin treatment prevents STZ-induced changes in the activities of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naringerin; Hypoglycaemic activity; Streptozotocin; Diabetes mellitus; Aminotransferases; Hepatic; Cardiac tissues.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100007

Metabolic and morphometric alterations inherent to neuromuscular electric stimulation in the antagonist muscle submitted to ankle joint immobilization
Provedor de dados: 52
Autores: Durigan,João Luiz Quagliotti; Cancelliero,Karina Maria; Bosi,Paula Lima; Delfino,Gabriel Borges; Montebelo,Maria Imaculada de Lima; Guirro,Rinaldo Roberto de Jesus; Silva,Carlos Alberto da; Polacow,Maria Luiza Ozores.
The aim of this work was to investigate the effect of the neuromuscular electrical stimulation (ES) on the metabolic and morfometric profile of the tibialis anterior muscle, antagonist to the soleus muscle which was stimulate, under the joint immobilization condition of the ankle for 7 and 15 days. The immobilization promoted the reduction in the muscle mass (I7: 17.36%, I15: 20.83%), in the glycogen content (I7: 48%, I15: 48%), in the muscle fibers areas (I7: 27%, I15: 40%) and increase in intramuscular connective tissue density (I7: 122%, I15: 206%). The EE didn't promote significant alterations in the mass of the immobilized groups, however, promoted increase in the glycogen (IEE7: 31.25%; IEE15: 56.25%), reduction in the muscle fibers areas (IEE7: 14%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Muscle disuse; Metabolic; Morphometric; Neuromuscular electrical stimulation; Rehabilitation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100011

Overview of parameters influencing biomass and bioreactor performance used for extracellular ligninase production from Phanerochaete chrysosporium
Provedor de dados: 52
Autores: Ntwampe,Seteno; Chowdhury,Faysol; Sheldon,Marshall; Volschenk,Heinrich.
The production of extracellular enzymes is gaining momentum as commercial interests seek alternative ways to improve the productivity in the biotechnology and pharmaceutical industries. Early research studies looked at improving batch bioreactor operational challenges; however, the use of continuous cultures was indicated to be favourable. This led to a new approach developed to produce extracellular enzymes continuously using fixed-film bioreactors from biofilms immobilised on polymeric and inorganic membranes. In this review, the performance of P. chrysosporium biomass, evaluated in terms of ligninase production using different bioreactor operation conditions, is highlighted. Furthermore, the limitations related to the implementation of optimised batch...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phanerochaete chrysosporium; Lignin peroxidase; Manganese peroxidase; Dissolved oxygen; Bioreactors.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500008

Feature Level Two -Dimensional Arrays Based Fusion in the Personal Authentication system using Physiological Biometric traits
Provedor de dados: 52
Autores: Carol .J,Jerusalin; Fred,A. Lenin; Daniel,Ashy.V.
ABSTRACT The fingerprint, knuckle print and the retina are used to authenticate a person accurately because of the permanence in the features. These three biometric traits are fused for better security. The fingerprint and knuckle print images are pre-processed by morphological techniques and the features are extracted from the normalized image using gabor filter. The retinal image is converted to gray image and pre-processing is done using top hat and bottom hat filtering. Blood vessels are segmented and the features are extracted by locating the optic disk as the centre point. The extracted features from the fingerprint, knuckle print and the retina are fused together as one template and stored in the data base for authentication purpose, thus reducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fingerprint; Knuckle print; Retina; Blood vessel segmentation; Feature level Fusion; Template.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300408

Coprophilous fungi from Brazil
Provedor de dados: 52
Autores: Richardson,Michael J.
Thirty-two species of coprophilous fungi were recorded from seven dung samples collected from the state of Matto Grosso do Sul, Brazil, and incubated in moist chambers. Descriptions of some of the more interesting fungi are given, and aspects of their biodiversity and ecology are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascomycetes; Coprophilous fungi; Diversity; Species richness.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300010

Determination of 4-vinylgaiacol and 4-vinylphenol in top-fermented wheat beers by isocratic high performance liquid chromatography with ultraviolet detector
Provedor de dados: 52
Autores: Zhu,Mingguang; Cui,Yunqian.
This work aimed at determining 4-vinylguaiacol and 4-vinylphenol in the top-fermented wheat beers using different wavelength and the mobile phase for HPLC. Best results for isocratic elution were obtained at 260 nm and the mobile phase comprising methanol/ultrapure water/phosphoric acid (400/590/10, V/V). Under these conditions, the retention time of 4-vinylguaiacol and 4-vinylphenol was 25 and 27min, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-vinylguaiacol; 4-vinylphenol; Top-fermented wheat beers; HPLC; Phenolic off-flavor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600018

Repellent, antifeedant and insecticidal effects of neem oil on Microtheca punctigera
Provedor de dados: 52
Autores: Mikami,Adriana Yatie; Ventura,Maurício Ursi.
The effects of concentrations (0.00, 0.25, 0.50 and 1.00%) of the neem tree (Azadirachta indica - Meliaceae) oil emulsion on the behavioral and biological parameters of M. punctigera were investigated in the laboratory. Wild radish (Raphanus raphanistrum L.) host plant was used. Multiple and no-choice feeding preference assays were conducted which shown multiple effects. The males were repelled by the neem oil in multiple-choice assay. The adult (multiple-choice) and larvae (multiple and no-choice) feeding were deterred. The larvae mortality was higher in the neem oil treated than the control leaves. Further investigations are suggested to test neem oil in the management of the pest in the field.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Yellowmargined leaf beetles; Botanical insecticide.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600006

Ultra-structural and histochemical analysis of channel catfish (Ictalurus punctatus) liver treated with fumonisin B1
Provedor de dados: 52
Autores: Scaff,Rejane Maria Cirra; Scussel,Vildes Maria.
The histopathological effects of fumonisin B1 (FB1) injected intraperitoneally (IP), was evaluated in catfish (Ictalurus punctatus). Fishes were divided into four Groups. Groups II, III and IV were treated IP with FB1 injections of 1; 5 and 10 mg/kg bw/day, respectively, during 21 days. At the 7th, 14th and 21st day, fishes were sacrificed. The livers were hystologicaly analysed by the light and transmission electronic microscopy. Livers from the 7th day showed organelles alterations, particularly in the granular endoplasmatic reticle, mitochondria, nucleus and nucleolus mediated by FB1 doses. The occurrence of processes involved in the necrosis and apoptosis was detected. At the highest FB1 dose,the livers presented an intense response with an accentuate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver; Catfish; Fumonisin B1; Histopathology; Ultra-structural microscopy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200013

Bubaline Diaphragm Matrix: Development and Clinical Assessment into Cattle Abdominal Hernia Repair
Provedor de dados: 52
Autores: Vora,Shruti Dineshbhai; Kumar,Vineet; Asodiya,Foram Arvindbhai; Singh,Vivek Kumar; Fefar,Dhaval Tribhovanbhai; Gajera,Harsukh Popatbhai.
Abstract The purpose of the study was to develop a xenogenic bubaline diaphragm matrix (BDM) for abdominal hernia repair. A fresh diaphragm was decellularized using aqueous sodium dodecyl sulfate (SDS) solutions (0.5-4% w/v) over a period. Acellularity was confirmed histologically and characterized by Masson’s trichrome staining, scanning electron microscopy (SEM), DNA quantification, agarose gel electrophoresis, and Fourier-transform infrared spectroscopy. The BDM was used for clinical abdominal hernia repair in six cattle. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BDM. Histologically, the diaphragm treated with 2% SDS for 48 h showed complete acellularity and orderly arranged collagen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biocompatibility; Bubaline diaphragm matrix; DNA quantification; Spectroscopy; Scanning electron microscopy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100309

Characterization of chestnut (Castanea sativa, mill) starch for industrial utilization
Provedor de dados: 52
Autores: Demiate,Ivo Mottin; Oetterer,Marília; Wosiacki,Gilvan.
Studies were conducted to characterize the chestnut and its starch. Chemical composition of the chestnuts showed high level of starch. Moisture level in the raw nuts was around 50g/100g in wet basis and starch content, around 80g/100g in dry basis; other nut flour components were protein (5.58 g/100g), lipid (5.39 g/100g), crude fiber (2.34 g/100g) and ash (2.14 g/100g). Starch fraction was chemically characterized in order to identify the granule quality as compared with those of cassava and corn. This fraction showed more lipids and proteins than the other starches. Chestnut starch granules showed peculiar shape, smaller than the control starches and low amount of damaged units. Chemical composition concerning amylose : amylopectin ratio was intermediate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Castanea sativa; Starch; Granules; Pastes; Sweet Chestnut; Cassava.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100010

Growth capacity of thermotolerant campylobacters in culture media supplemented with pig and cow blood
Provedor de dados: 52
Autores: Tresierra-Ayala,Álvaro; Navas,Manuel; Flores,Josué; Perea,Ramsés; Huanaquiri,Juan; Bendayán,María; Fernández,Heriberto.
In this work 60 thermotolerant Campylobacter strains (37 C. jejuni and 23 C. coli) isolated from the cows, pigs, chickens and ducks (15 strains of each type of animal) were used to establish their growth capacity on media containing cow or swine blood as potential substitutes of sheep or horse blood. The growth capacity was assessed by viable counts on cow and swine blood media, using the modified Miles and Misra method. Campylobacter strains showed better growth in the media supplemented with pig or sheep blood than with cow blood. Thus, the use of pig blood could be a supplement for Campylobacter culture medium, when there was no availability of sheep or horse blood.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter; Culture media; Cow blood; Swine blood.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500011

Colonization of the Corumbá Reservoir (Corumbá River, Paraná River Basin, Goiás State, Brazil) by the "lambari" Astyanax altiparanae (Tetragonopterinae; Characidae)
Provedor de dados: 52
Autores: Dias,Rosa Maria; Bailly,Dayani; Antônio,Rosimeire Ribeiro; Suzuki,Harumi Irene; Agostinho,Angelo Antonio.
This work aimed to determine which population features were important to the colonization process of Astyanax altiparanae in the Corumbá Reservoir. The species presented wide spatial-temporal distribution, caught in all months and sites. Higher CPE values were recorded upstream from the reservoir during the filling phase. In this phase, reproductive activity was intense upstream and moderate in the tributaries. However, a fall in abundance of juveniles was observed after the formation of the reservoir. Among the most relevant population features to grant a successful colonization were wide geographic distribution, ability to occupy and spawn in lentic habitats and feeding flexibility.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax altiparanae; Reservoir; Colonization; Reproduction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300017

A Review of Scientific Papers About Head and Neck Cancers
Provedor de dados: 52
Autores: Paoli,Severo de; Fonseca,Adenilson de Souza da; Paoli,Flávia de; Geller,Mauro; Presta,Giuseppe Antonio; Santos-Filho,Sebastião David; Bernardo-Filho,Mário.
Head and neck cancer is one of the 10 most frequent cancers worldwide, with an estimated 500000 new cases diagnosed annually. Treatment of head and neck cancers require a multidisciplinary approach due their complexity and the functional and esthetic alterations that cancer can cause. The interest of the scientific community in a specific subject can be evaluated by analyzing of the number and the quality of published papers on the topic. The information obtained from PubMed (www.ncbi.nlm.nih.gov/sites/entrez) has been used as a tool in various publications to aid the evaluation of the scientific interest in specific research areas The aim of this work is to evaluate, using PubMed, the scientific interest in studies of head and neck cancer treatments such...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scientific interest; PubMed; Cancer; Surgery; Radiotherapy; Chemotherapy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700011

Parasitism Capacity of Trichogramma pretiosum and Trichogramma acacioi (Hym.: Trichogrammatidae) on eggs of Sitotroga cerealella (Lep.: Gelechiidae)
Provedor de dados: 52
Autores: Pratissoli,Dirceu; Zanuncio,José Cola; Vianna,Ulysses Rodrigues; Andrade,Josimar Souza; Zinger,Fernando Domingo; Alencar,João Rafael de Conte Carvalho de; Leite,Germano Leão Demolin.
The objective of this work was to evaluate the parasitism capacity of Trichogramma pretiosum Riley and T. acacioi Brun, Moraes and Soares (Hymenoptera: Trichogrammatidae) in eggs of the alternative host Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae) aiming to use both species in biological control programs of Nipteria panacea Tierry-Mieg (Lepidoptera: Geometridae). The parasitism rhythm and total parasitism of these parasitoid species were affected by the temperature with higher values during the first 24 h of their life. Parasitism period was longer for T. pretiosum and T. acacioi at the lowest temperature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avocado; Insecta; Trichogrammatidae; Temperature.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600021

Gonadosomatic index and some hematological parameters in African catfish  Clarias gariepinus (Burchell, 1822) as affected by feed type and temperature level
Provedor de dados: 52
Autores: Al-Deghayem,Waleed A.; Al-Balawi,Hamoud F.; Kandeal,Saleh A.; Suliman,El Amin M.
ABSTRACT The purpose of the current study was to examine the effects of different feeds/nutrients and temperature on the gonadal development of Clarias gariepinus. The gonadosomatic index (GSI) and blood parameters including red blood cell count (RBCs), white blood cell count (WBCs), hemoglobin (Hgb) level, hematocrit (HCT), platelets (PLT) and mean corpuscular volume (MCV) were investigated. Four types of fish feed of 36% protein content [D1(fish meal), D2 (soybean meal), D3 (peameal) and D4 (commercial tilapia feed)] and 3 different levels of temperature (T1(24℃), T2(28℃) and T3(32℃) were tried in this study for a duration of 4 months. The mean values were as follows: female GSI (14.68 ± 4.86), male GSI (0.70 ± 0.32), RBCs x 106(2.45± 0.64), WBCs x...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feed; Temperature; Clarias gariepinus; Hematology; GSI.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100404

Competition between Catolaccus grandis (Hymenoptera: Pteromalidae) and Bracon vulgaris (Hymenoptera: Braconidae), parasitoids of the Boll Weevil
Provedor de dados: 52
Autores: Ramalho,Francisco de Sousa; Silva,Ana Maria Camêlo da; Zanuncio,José Cola; Serrão,José Eduardo.
The competition between populations of the parasitoids C. grandis and B. vulgaris was studied using larvae of Euscepes postfasciatus (Fairmaire) as an alternative host. A series of biological parameters was observed and related to the competitive abilities of both parasitoid species. They were capable of colonizing and maintaining their populations regardless of host location. The population growth of C. grandis and B. vulgaris, based on fecundity was not affected by the competition. The parasitism and survivorship to the adult stage were affected by competition, except when the host was located at the bottom of the rearing cage. C. grandis performed better than B. vulgaris independently of the competition and host location, but it did not exclude the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Anthonomus grandis; Competition between parasitoids.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300002

Feeding and trophic ecomorphology of Satanoperca pappaterra (Pisces, Cichlidae) in the Manso Reservoir, Mato Grosso State, Brazil
Provedor de dados: 52
Autores: Hahn,Norma Segatti; Cunha,Fabiane.
The aim of this work was to evaluate the relationship between diet and features of the trophic ecomorphology of Satanoperca pappaterra (Heckel, 1840) in an impacted environment. Samples were collected from March 2000 to February 2003 in Manso Reservoir, Cuiabá River, Mato Grosso State. Analysis of 93 stomachs contents showed that food resources associated with the substrate, such as plant remains, detritus, fish scales and Chironomidae larvae, dominated the diet. Consequently this species was characterized as a detritivorous-invertivorous. However, individuals collected in 2003 showed a greater selection of benthic organisms in relation to previous periods. Morphological structures such as position of the mouth, form of the lips, gill rakers and pharyngeal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cichlidae; Diet; Morphology; Freshwater.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800017

Contribuição ao Estudo da Planície Litorânea do Estado do Paraná
Provedor de dados: 52
Autores: Bigarella,João José.
The present paper contains an approach to systematize the study of the State of Paraná coastal plains, in the southern part of Brazil. It contains in general some geographical and geological data, documented by maps, sketches and photographs. In the initial stage the coastal plain represents a marine ingression, caused by faulting. This ingression penetrated the valleys of a not yet determinated geological landscape. Ended the movement of deeping by faulting begins the epirogenic ascension and building of the barriers and beach ridges. An intensive sedimentation caused the lagoons and bays obstruction. This work refers mainly to t33 he sedimentary formations and the coastal plains is classified in the following way for the study in consideration to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Costal plains; State of Paraná; Marine sedimentation; Mangrove swamps; Mangrovito; Continental sedimentation; Eolic dunes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500005

Production of tannase by Aspergillus tamarii in submerged cultures
Provedor de dados: 52
Autores: Costa,Andréa M.; Ribeiro,Wanessa X.; Kato,Elaine; Monteiro,Antonio Roberto G.; Peralta,Rosane Marina.
The production of tannase by Aspergillus tamarii was studied in submerged cultures. The fungus produced an extracellular tannase after two days of growth in mineral medium containing tannic acid, gallic acid and methyl gallate as carbon source. The best result was obtained using gallic acid as inducer (20.6 U/ml). The production of enzyme was strongly repressed by the presence of glucose. Crude enzyme was optimally active at pH 5.0 and 30º C. The enzyme was stable in a large range of pH and up to the temperature of 45º C.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus tamarii; Enzyme; Fungal biomass; Submerged cultures; Tannase; Tannins.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200021

Measurement of volatilized mercury by a mini-system: a simple, reliable and reproducible technique
Provedor de dados: 52
Autores: Cursino,Luciana; Mattos,Silvânia V. M.; Silva,Nilton O.; Chartone-Souza,Edmar; Nascimento,Andréa M. A.
A simple and easy new technique for volatilized mercury determination in biological systems was developed. This technique is fast and sensitive and can overcome the problems that arise due to the extremely low readings during the measurements and reproducibility in biological material (bacteria). It measures directly the volatilized metallic mercury of bacteria by means of a chemical adsorbent in a coupled mini-system, as a modified technique for mercury in air analysis. It is potentially of interest to the bioremediation and bacterial mercury resistance communities
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Biological system; Measurement; Mercury; Volatilization.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400028

Morphometric patterns of two fouling Eudendrium spp. (Hydrozoa, Anthomedusae, Eudendriidae) from São Sebastião (SP, SE Brazil)
Provedor de dados: 52
Autores: Oliveira,Otto M.P.; Marques,Antonio C.; Migotto,Alvaro E.
Thirty colonies of Eudendrium carneum and 100 colonies of E. glomeratum, collected on recruitment panels in São Sebastião (SP, SE Brazil), were studied concerning morphometrical parameters and compared with morphometrical data available from other locations. The results show that E. carneum from Brazil has a relatively smaller morphometric variation than colonies of E. glomeratum for Brazil and other areas. Both morphological and speciation causes are suggested as possible explanations for the high variation of E. glomeratum.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrozoa; Eudendrium morphometry taxonomy Brazil.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500012

Antifungal Potential of Indigenous Medicinal Plants against Myrothecium Leaf Spot of Bitter Gourd ( Momordica charantia L.)
Provedor de dados: 52
Autores: Abid,Muhammad; Chohan,Sobia; Mehmood,Mirza Abid; Naz,Safina; Naqvi,Syed Atif Hasan.
ABSTRACT  Bitter gourd is of great importance due to its usage against the treatment of numerous ailments in human beings. A comprehensive survey at four localities of Southern Punjab, Pakistan was carried out to determine the severity of Myrothecium leaf spot. Maximum disease severity was at C1 (Chak 11/NP) and least at C2 (Kot Mehtab). Among isolated species Myrothecium roridum was found more prevalent and pathogenic as compared to M. verrucaria. Antifungal activity using solvent extracts of five medicinal plants (Mangifera indica, Melia azedarach, Nicotiana tabacum, Moringa oleifera and Eucalyptus globosum) were evaluated against isolated species by agar well diffusion method at various concentrations (0.01, 0.10, 1.0 and 10.0 µg / mL). N. tabacum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Momordica charantia L.; Secondary compounds; Solvent plant extracts.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100421

Effects of limnological and morphometric factors upon Zmin, Zmax and width of Egeria spp stands in a tropical reservoir
Provedor de dados: 52
Autores: Pierini,Sandra Andréa; Thomaz,Sidinei Magela.
In this work, regression analyses were used to test the effects of fetch, littoral slope and Secchi disk depth upon the stand width (Wi) and maximum (Zmax) and minimum (Zmin) colonization depths of Egeria spp in a large tropical reservoir (Rosana Reservoir). Littoral slope was the only variable correlated with Wi, which was larger in locations with lower slopes. The higher Wi values (up to 260m) were found in locations where slopes were lower than 0.05 m.m-1. Zmin and Zmax were correlated positively with fetch. Nevertheless, different relationships were found at lower and higher fetch values observed in the arms and in the main body of the reservoir, respectively. At lower values, Secchi disk depth was the main variable explaining Egeria Zmax. On the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egeria; Fetch; Littoral slope; Secchi disk.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200016

Molecular Modeling, Structural Analysis and Evaluation of 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase (DXR) as a Putative Drug Target For Theileria Parva
Provedor de dados: 52
Autores: Icen-Taskin,Irmak; Munzuroglu,Omer.
ABSTRACT The apicomplexan parasite Theileria parva, the causative agent of ECF, is an important pathogen affecting both domestic and wild animals, causing major economic losses in the world. Problems such as high cost of drugs, development of resistance, and absence of effective vaccines prevent effective combating of the pathogen. Thus, it is necessary to explore new targets for affordable and higher therapeutic value drugs. 1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) in the non-mevalonate isoprene biosynthesis pathway is vital to the organism and therefore has been selected as a target for developing antitheilerial drugs. In this study, the 3D structure of TpDXR was identified by template-based in silico homology modelling method, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homology modelling; Theileria parva; 1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR); East Coast Fever.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100458

Glycolytic activities in size-fractionated water samples: emphasis on rhamnosidase, arabinosidase and fucosidase activities
Provedor de dados: 52
Autores: Colombo-Corbi,Vanessa; Dellamano-Oliveira,Maria José; Vieira,Armando Augusto Henriques.
Glycolytic activities of eight enzymes in size-fractionated water samples from a eutrophic tropical reservoir are presented in this study, including enzymes assayed for the first time in a freshwater environment. Among these enzymes, rhamnosidase, arabinosidase and fucosidase presented high activity in the free-living fraction, while glucosidase, mannosidase and galactosidase exhibited high activity in the attached fraction. The low activity registered for rhamnosidase, arabinosidase and fucosidase in the attached fraction seemed contribute to the integrity of the aggregate and based on this fact, a protective role for these structures was proposed. The presented enzyme profiles and the differences in the relative activities probably reflected the organic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eutrophic reservoir; Extracellular polysaccharides; Phytoplanktonic bloom; Microbial activity; Size-fractionated samples.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300013

Arginine specific aminopeptidase from Lactobacillus brevis
Provedor de dados: 52
Autores: Nandan,Arya; Gaurav,Amit; Pandey,Ashok; Nampoothiri,Kesavan Madhavan.
The proteolytic system of lactic acid bacteria contribute to the development of flavor during the ripening of cheese through the generation of short peptides and free amino acids, which directly or indirectly act as flavor precursors. Newly isolated lactic acid bacteria (LAB) as well as those procured from culture collection centers were screened for the production of various substrate specific aminopeptidases. Among all the strains screened, L. brevis (NRRL B-1836) was found to produce quantifiable amount of intracellular arginine specific aminopeptidase (EC 3.4.11.6). The productivity of arginine aminopeptidase in 5 L fermentor was 36 IU/L/h. The Luedeking and Piret model was tested for intracellular production of aminopeptidase and the data seemed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus brevis; Aminopeptidases; Arginine- p- nitroanilide.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600021

Simultaneous determination of Pb and Cd in seafood by ICP OES with on-line pre-concentration by solid phase extraction with amberlite XAD-4 after complex formation with DDTP
Provedor de dados: 52
Autores: Santos,Éder José dos; Santos,Arlene Bispo dos; Herrmann,Amanda Beatriz; Kulik,Suzete; Baika,Loana Mara; Tormen,Luciano; Curtius,Adilson José.
An on-line method for Cd and Pb pre-concentration and simultaneous determination in acid digested seafood by inductively coupled plasma optical emission spectrometry (ICP OES) was developed. The on-line pre-concentration was based on the complex formation of the analytes with the ammonium salt of O,O-diethyldithiophosphate (DDTP) and using the Amberlite XAD-4 resin as a solid support in a homemade column. Different conditions of the flow injection system, such as solutions flow rates, nebulizer pressure and eluent concentration were optimized. Three certified reference materials of lobster hepatopancreas, dogfish liver and fish protein, three samples of fish muscle and three samples of shrimp were digested with HNO3, H2O2 and H2SO4 in a microwave system...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cd; Pb; ICP OES; Diethyldithiophosphate; XAD-4; Seafood; Solid phase extraction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100017

Oxidative stress action in cellular aging
Provedor de dados: 52
Autores: Oliveira,Monique Cristine de; Schoffen,João Paulo Ferreira.
Various theories try to explain the biological aging by changing the functions and structure of organic systems and cells. During lifetime, free radicals in the oxidative stress lead to lipid peroxidation of cellular membranes, homeostasis imbalance, chemical residues formation, gene mutations in DNA, dysfunction of certain organelles, and the arise of diseases due to cell death and/or injury. This review describes the action of oxidative stress in the cells aging process, emphasizing the factors such as cellular oxidative damage, its consequences and the main protective measures taken to prevent or delay this process. Tests with antioxidants: vitamins A, E and C, flavonoids, carotenoids and minerals, the practice of caloric restriction and physical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Cellular aging; Cell death; Oxidative stress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600009

Selection of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. strains for control of Cornitermes cumulans (Kollar)
Provedor de dados: 52
Autores: Neves,Pedro J.; Alves,Sérgio Batista.
Fifty strains of the entomopathogenic fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. were tested against the termite Cornitermes cumulans. In the first phase of the experiments, several bioassays were conducted and the five best strains were selected. The criterion for strain selection during this phase was the confirmed mortality above 50% five days after application of the fungus. Three M. anisopliae and two B. bassiana strains were the most virulent. The second phase of the experiments consisted of a bioassay and a conidial production test using a rice medium. The best M. anisopliae and B. bassiana strains were selected using both the confirmed insect mortality nine days after application of the fungus and the yield...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomophatogenic fungi; Termits; Biological control.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400004

Sobre a Dosagem Fotométrica do Cobre em Águas
Provedor de dados: 52
Autores: Spitzner,Reinaldo.
The author presents an analytic process for copper quantitative determination in mineral and potable waters, based in the reaction of the cation copper with sodium diethyl-dithio carbamate. Due to the presence of certain elements common in water, such as iron, calcium, magnesium, etc., the main principles of the reactions behavior were studied. In the same manner the reaction sensibility was verified, as well as its precision, by the addition of known quantities of copper. About the end of the work presented, there are shown some copper determinations made through the mentioned process with mineral and potable waters of Paraná.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copper; Photometry; Mineral waters.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500004

Aqueous extract of Huang-lian induces apoptosis in lung cancer cells via P53- mediated mitochondrial apoptosis
Provedor de dados: 52
Autores: Zhu,Deying; Lin,Zhe; Li,Zhenzhong.
The current study was designed to evaluate the activity of the aqueous extract of Huang-lian, and the main apoptosis pathway induced by the extracts of Huang-lian was detected on lung cancer. Antiproliferactive activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TUNEL methods against human lung cancer cells (A549). Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial pathway via p53 and was detected by western blot. It increased the activation of caspase-3 and caspase-3 cleavage increased as the time increased. These results suggested that Huang-lianregulated the Bcl-2 family protein-mediated mitochondrial via p53 pathway, suggesting that Huang-lian should be further investigation as a natural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Huang-lian; Aqueous extract; Apoptosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300353

Structure and Dynamics of Fish Assemblages in a Tidal Creek Environment
Provedor de dados: 52
Autores: Vendel,Ana Lúcia; Spach,Henry Louis; Lopes,Sabine Granado; Santos,César.
Studies were carried out on structure and dynamics of fish assemblages in the Baguaçu tidal creek, Paranaguá Bay, Brazil. A total of 30,104 fish were captured, comprising 21 families and 47 species. Both in weight and in number, the species Anchoa parva prevailed. Monthly captures in number and weight were largest in the autumn and part of the winter. No seasonal tendency was observed in the indexes of community structure. The dendrogram produced by the classification of the samples separated the 12 months of collection into three groups, reflecting differences in the qualitative and quantitative occurrences of the most important taxa. Some ecological likeness, not only seasonal patterns of abundance, seemed evident in the seasonality of the groupings of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tidal creek; Fish assemblages; Seasonal variation; Brazil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300015

Dimethoate degradation in plants and during processing of yerba maté leaves
Provedor de dados: 52
Autores: Schmalko,Miguel E.; Ramallo,Laura A.; Ferreira,Darío; Berlingheri,Rubén D.
The objective of this research was to study degradation kinetics of dimethoate in plants of Ilex paraguariensis Saint Hilaire (or yerba maté) and during its processing. To determine dimethoate concentration, a capillary gas chromatography technique with a mass selective detector was used. Half-life times in plants ranked between 9.8 and 11.8 days. During processing, with a blanching and two drying steps, dimethoate concentration decayed to a 22.7% of its initial value (in dry basis); while during seasoning step (at 45°C), half-life time was 17.3 days. With these values, preharvest safety interval was determined.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dimethoate; Pesticide; Degradation; Processing; Ilex paraguariensis; Yerba maté.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600003

Evaluation of activity of the crude ethanolic extract of Magonia pubescens St. Hil (Sapindaceae) against larvae of the cattle tick Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Acari: Ixodidae)
Provedor de dados: 52
Autores: Fernandes,Fernando de Freitas; Bessa,Priscilla Antunes Diógenes; Freitas,Edméia de Paula e Souza.
The acaricidal potential of the crude ethanolic extract (c.e.e.) of the stem peel of Magonia pubescens was evaluated against larvae of Rhipicephalus (Boophilus) microplus. The larvae were placed in filter paper envelopes impregnated with different concentrations of c.e.e., dissolved in dimethylsulfoxide (DMSO) and distilled water for determination of lethal concentrations (LC). The following treatments were used: 1. Envelopes of dry filter paper; 2. Envelopes of filter paper moistened with distilled water; 3. Envelopes of filter paper moistened with a solution of DMSO in distilled water; and 4. Envelopes moistened with 2 mL of each concentration of the c.e.e. to be tested. The bioassays were carried out in quadruplicate at 27°±1° C and RH e"80% and 12h...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botanical acaricide; Sapindaceae; Magonia pubescens; Tinguí-do-cerrado; Boophilus microplus; Cattle tick.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600009

Polymerase chain reaction (PCR) identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province
Provedor de dados: 52
Autores: Demirel,Rasime; Sariozlu,Nalan Yilmaz; İlhan,Semra.
In the present study, nine terverticillate Penicillium isolates (P. griseofulfum, P. puberulum, P. crustosum, P. aurantiogriseum, P. chrysogenum, P. primulinum, P. expansum, P. viridicatum, Eupenicillium egyptiacum) from 56 soil samples were characterized genetically by a PCR method. The DNAs of the strains were isolated using the glass beads and vortexing extraction method and then used for PCR amplification with the internal transcribed spacer 1 (ITS1) and ITS4 universal fungal specific primers. The ITS regions of fungal ribosomal DNA (rDNA) were sequenced through the CEQ 8000 Genetic Analysis System. ITS-5.8S sequences obtained were compared with those deposited in the GenBank Database. The results indicated that the identification of Penicillium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium; Identification methods; PCR; ITS.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600013

Chromosomal differentiation between populations of Oligosarcus hepsetus (Teleostei, Characidae) from small tributaries at opposite margins of the Paraíba do Sul river (Brazil)
Provedor de dados: 52
Autores: Centofante,Liano; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando.
Cytogenetic analyses were carried out in two populations of Oligosarcus hepsetus from tributaries at opposite margins of the Paraíba do Sul river. The same diploid number was observed in both populations (2n=50), but they showed remarkable differences related to karyotype formula and distribution of rDNA sites as revealed by silver nitrate staining and in situ hybridization with 18S probes. The results suggested that the main channel of the Paraíba do Sul river acted as barrier to gene flow between populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Headstream; Cytogenetic; Differentiation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700016

Applications of nucleoside-based molecular probes for the in vivo assessment of tumour biochemistry using positron emission tomography (PET)
Provedor de dados: 52
Autores: Wiebe,Leonard I.
Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three dimensional images of radiotracer distribution in the body. Molecular imaging with PET refers to the use of positron-emitting biomolecules that are highly specific substrates for target enzymes, transport proteins or receptor proteins. Molecular imaging with PET produces spatial and temporal maps of the target-related processes. Molecular imaging is an important analytical tool in diagnostic medical imaging, therapy monitoring and the development of new drugs. Molecular imaging has its roots in molecular biology....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Molecular imaging; PET; Nucleosides; Nucleotides; Nucleic acids; Oligonucleotides; Antisense; Aptamer; Spiegelmer.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300011

Seasonal variability of coastal water quality in bay of Bengal and Palk Strait, Tamilnadu, Southeast Coast of India
Provedor de dados: 52
Autores: Srinivasan,Viswanathan; Natesan,Usha; Parthasarathy,Anitha.
The aim of this work was to study the physicochemical parameters of water quality collected from 12 sampling stations from Topputhurai to Muthupet in Vedaranyam located on the southeast coast of India from January to December 2008. Results showed that the DO and nutrients were the maximum in the Bay of Bengal during the monsoon period. High concentration of the nutrients in summer season was obtained near the Muthupet mangroves compared to the Palk Strait, which showed that this acted as a source of nutrients to the adjacent coastal waters. Low concentrations of the nutrients observed in the monsoon could be attributed to the terrestrial runoff from Muthupet lagoon. The physicochemical characteristics of coastal waters between the Point Calimere and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal zone; Physico-chemistry of water; Nutrients; Vedaranyam; Bay of Bengal; Palk Strait.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500020

Randomly Amplified Polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani
Provedor de dados: 52
Autores: Góes,Larissa Brandão; Costa,Ana Bolena Lima da; Freire,Laurineide Lopes de Carvalho; Oliveira,Neiva Tinti de.
Random Amplified Polymorphic DNA (RAPD) procedure was used to examine the genetic variability among fourteen isolates of Trichoderma and their ability to antagonize Rhizoctonia solani using a dual-culture assay for correlation among RAPD products and their hardness to R. solani. Seven oligodeoxynucleotide primers were selected for the RAPD assays which resulted in 197 bands for 14 isolates of Trichoderma. The data were entered into a binary matrix and a similarity matrix was constructed using DICE similarity (SD) index. A UPGMA cluster based on SD values was generated using NTSYS (Numerical Taxonomy System, Applied Biostatistics) computer program. A mean coefficient of similarity obtained for pairwise comparisons among the most antagonics isolates was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; Trichoderma; Rhizoctonia solani.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200005

Effect of mannitol on the pharmacokinetics of amikacin in wistar rats
Provedor de dados: 52
Autores: Olguín,Hugo Juárez; Portugal,Miriam Carrasco; Pérez,Janett Flores; Vieyra,Angélica Camacho; Pérez,Carmen Flores; Rodríguez,Alfonso Alfaro.
The study analyzed the effect of mannitol on the pharmacokinetics (PK) of amikacin. Adult Wistar rats were treated as follows: Group 1 (G1) received mannitol for three days, Group 2 (G2) received mannitol plus 10 mg/kg of amikacin simultaneously, and Group 3 only amikacin. The PK study was conducted on the 4th day. For which, blood samples were drawn at fixed times during 24 h and immunoenzymatically analyzed. Results revealed significant differences (p&lt;0.05) between the groups, e.g. Cmax were 62.26 ± 15.75 µg/ml for G1, 72.63 ± 24.80 µg/ml for G2 and 68.61 ± 27.40 µg/ml for G3. The AUC also differed in the three groups, being largest for G2, 222.52 ± 47.30 µg/ml/h, and smallest for G1, 135.59 ± 39.00 µg/ml/h. Alteration of the PK parameters observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacokinetics; Interactions; Amikacin; Mannitol; Antibiotics; Diuretics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400006

Utilisation of respirometry to assess organic matter reduction of effluents from the Camaçari industrial complex (BA, Brazil)
Provedor de dados: 52
Autores: Oliveira,Carla A.; Araújo,Cristiano V. M.; Nascimento,Renato B.; Strotmann,Uwe J.; Silva,Eduardo M. da.
The treatment efficiency of industrial effluents, after biological treatment by activated sludge in aeration tanks (AT), was assessed through the utilisation of respirometry tests at the Cetrel's-wastewater treatment plant (WTP). Samples of the equalised effluent (EE), prior to treatment, and of the treated effluent (TE), after treatment, were analysed. Twenty bioassays batch were carried out to AT (AT-2, AT-3 and AT-4). Each test consisted of: a basic test, a basic test with peptone added, a test using EE and a test using TE. The data showed that there was no statistically significant difference (p&gt;0.05) in the respiration activity between the aeration tanks. Regarding the specific oxygen uptake rate there was a mean reduction of 70.8% between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activated sludge; Bacterial toxicity; Oxygen uptake rate; Wastewater treatment plant.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200016

Biological evaluation of crude and degummed oil from Moringa oleifera seeds
Provedor de dados: 52
Autores: Fátima Andrade,Graziela de; Melo,Tânia Márcia Sacramento; Guedes,Cláudia Dumans; Novack,Kátia Monteiro; Santos,Rinaldo Cardoso dos; Silva,Marcelo Eustáquio.
The aim of this work was to evaluate the oil extracted from Moringa oleifera (fam. Moringaceae) seeds from the nutritional standpoint. Nutritional evaluation of crude or degummed moringa oil or soybean oil (as a control) involved the determination of the Food Efficiency (FE) in male Fisher rats and the fatty acid composition of the moringa oil. Hepatic and renal functions were assessed by measuring serum transaminases activity and urea and creatinine concentrations, respectively. Serum cholesterol and triglycerides, alkaline phosphatase, total proteins and albumine were also measured. Results showed that FE was slightly increased by the crude moringa oil while no differences were found between the soybean and degummed moringa oil regarding this parameter....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moringa oleifera oil; Degummed oil; Biological evaluation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500018

Modelling the growth of lactic acid bacteria at different temperatures
Provedor de dados: 52
Autores: Silva,Ana Paula Rosa da; Longhi,Daniel Angelo; Dalcanton,Francieli; Aragão,Gláucia Maria Falcão de.
ABSTRACT Mathematical models are widely used to predict the shelf life of foods. Lactic acid bacteria (LAB), particularly Lactobacillus plantarum, Weissella viridescens and Lactobacillus sakei, are the main spoilage bacteria of refrigerated, vacuum-packed meat products, stored in modified atmosphere, and their growth determines the shelf life length of these products. The objective of this study was to model the growth of L. plantarum, W. viridescens and L. sakei under different isothermal cultivation conditions and establish secondary models to describe the effect of temperature on the growth parameters of these bacteria. The LAB growth was evaluated in culture medium at temperatures of 4, 8, 12, 16, 20 and 30 ºC. The fit of Baranyi and Roberts (BAR) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predictive microbiology; Shelf life; Bacterial spoilage.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100411

Association between floral bud size and developmental stage in soybean microspores
Provedor de dados: 52
Autores: Lauxen,Mozart da Silva; Kaltchuk- Santos,Eliane; Hu,Ching -yeh; Callegari- Jacques,Sidia Maria; Bodanese-Zanettini,Maria Helena.
This study was carried out to establish the association between floral bud size and the corresponding microspore developmental stages for Brazilian soybean cultivars. Microspore developmental stage distributions were examined in young buds from cv Década, IAS5 and RS7. The data indicated that for a given bud-size group, the microspores of different cultivars were at different developmental stages, with cv RS7 and Década distributed at the youngest and cv IAS5 at the most advanced stages. Microspore stages distribution were also compared among the ten anthers of the same bud of the above cultivars. The ten anthers from a given bud were clearly distributed at different developmental stages. Caution should be exercised when adopting the standard anther...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Soybean; Microsporogenesis; Anther culture.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400004

Chemical composition and functional properties of malted corn flours
Provedor de dados: 52
Autores: Grossmann,Maria Victória E.; Mandarino,José Marcos G.; Yabu,Márcia Cristina.
Flours with different physicochemical and functional properties were obtained from malted corn. Response surface methodology (RSM) was used to study the effects of malting time (1,3, 5 days), malting temperature (20, 25, 30º C) and gibberelic acid concentration (0.0; 0.5; 1.0 %) on these properties. The chemical composition and paste viscosity of flours were significantly affected by malting time and temperature, while water solubility index (WSI) and water absorption index (WAI) varied only with malting time. Gibberelic acid did not significantly influenced any of the studied properties. Germination at 20-25ºC for 3 days was recomended to obtain corn flour with high WAI, low viscosity and medium WSI, without excessive loss of proteins.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malting; Corn flour; Functional properties.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200003

Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus
Provedor de dados: 52
Autores: Custodio,Luiz Antonio; Saito,Alexandre; Amarante,Marla Karine; Fujita,Thiago Cezar; Perim,Aparecida de Lourdes; Costa,Ivete Conchon; Felipe,Ionice; Jankevicius,Shiduca Itow.
In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium leprae; Leprosy; Hanseniase; Nasal mucus; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300007

Advantages and Applications of Cryopreservation in Fisheries Science
Provedor de dados: 52
Autores: Tsai,S.; Lin,C.
Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014

Fast assessment of mineral constituents in grass by inductively coupled plasma optical emission spectrometry
Provedor de dados: 52
Autores: Santos,Éder José dos; Baika,Loana Mara; Herrmann,Amanda Beatriz; Kulik,Suzete; Sato,Charyane Satie; Santos,Arlene Bispo dos; Curtius,Adilson José.
A method for evaluating inorganic constituents in the grass by ICP OES is proposed. The samples were digested with nitric acid plus hydrogen peroxide in a microwave system. Seventeen samples were collected in the metropolitan area of Curitiba and Castro in the Paraná State, Brazil. The following elements were studied: Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, P, Pb, Sb, Se and Zn. The linear coefficients of the curves were higher than 0.999 and the precision, expressed as the relative standard deviation, which was below 10%, being considered adequate. The accuracy was validated by using the recovery test (recoveries from 80 to 107%) and by the analysis of botanical certified materials. For one sample, two other digestion procedures, using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ICP OES; Inorganic constituents; Acid digestion; PCA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300017

Stability evaluation of propolis topical bases for veterinary use
Provedor de dados: 52
Autores: Farias,Luana d'Avila; Pereira,Camila Bugnotto; Machado,Gustavo; Schmidt,Cleber Alberto; Vargas,Agueda Castagna de.
This study aimed to evaluate and select different dermatological bases incorporated with propolis for veterinary use as well as to analyze the chemical compounds of the propolis hydroalcoholic extract by LC-MS/MS. Thus, formulations were submitted to accelerated stability tests under different temperatures and to mechanical stress, and evaluated for the appearance, color, odor, pH, viscosity, spreadability, and the mean size of the dispersed globules from the internal phase during a period of three months. The creamy gel formulation showed satisfactory results for all the evaluated items with an excellent capability to incorporate the hydroalcoholic extract of propolis associated to the maintenance of its physicochemical properties. The propolis used in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Accelerated stability; Phytotherapic; Caffeic acid.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600008

Isolation and Characterization of Midgut Lectin From Aedes aegypti (L.) (Diptera: Culicidae)
Provedor de dados: 52
Autores: Ayaad,Tahany Hassan; Al-Akeel,Rasha Khalifah; Olayan,Ebtisam.
ABSTRACT The present investigation deals with the isolation and characterization of a lectin from Aedes aegypti (Ae aegypti) female mid gut extract that agglutinates various mammalian red blood cells (RBCs) such as human three groups A, B, and O (RH+), mouse, rat, guinea-pig, sheep and goat erythrocytes. The highest activity of both crude and isolated mid gut lectins were detected against sheep RBCs. Using (NH4)2 SO4 fractionation, ion-exchange and mannose-CNBr-Sepharose 6B affinity chromatography techniques, Ae. aegypti midgut lectin (Aelec) was purified to homogeneity.Isoelectric focusing (IEF) and reducing SDS/PAGE revealed that the isolated mid gut lectin had isoelectric point (PI) of 5.90, and subunits approximate molecular weights of 35.50 and 27.35...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lectin; Purification; Characterization; Hemagglutination; Aedes aegypti; Mosquitoes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600905

Leaf surface characters applied to Lauraceae taxonomy in a Seasonal Forest of Southern Brazil
Provedor de dados: 52
Autores: Ceolin,Guilherme Bordignon; Rosito,Jumaida Maria; Canto-Dorow,Thais Scotti do.
The goal of this work was to test if the macro and micro morphological analysis of the leaf surface could provide vegetative diagnostic characters for some of the most common Lauraceae species in Southern Brazilian Seasonal Forests. The leaf printing technique with universal instantaneous adhesive was used for the epidermical microscopical analysis and external macroscospical analyses of leave were made. Microscopic evaluation revealed the visibility and contours of anticlinal walls of epidermical cell and stomata and shape of guard-cells. Macroscopic evaluation showed the absence or presence of characters such as hairiness, domatia and scents. The results showed that analyzed characters, together with other diagnostic characteristics, could contribute in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lauraceae; Leaf surface; Printing; Seasonal Forests; Stomata.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600017

A bovine teat papilloma specimen harboring Deltapapillomavirus (BPV-1) and Xipapillomavirus (BPV-6) representatives
Provedor de dados: 52
Autores: Claus,Marlise Pompeo; Lunardi,Michele; Alfieri,Amauri Alcindo; Otonel,Rodrigo Alejandro Arellano; Ferracin,Lara Munique; Fungaro,Maria Helena Pelegrinelli; Alfieri,Alice Fernandes.
The common occurrence of multiple papillomavirus infections has been shown in several studies involving the human host. However, investigations with the aim of identifying mixed papillomavirus infections in cattle have been conducted only recently. In the current work we describe a co-infection with two different bovine papillomavirus (BPV) types that was identified in a bovine teat papilloma. The skin wart was obtained from a cow belonging to a Brazilian beef herd. A PCR assay was carried out with the FAP primer pair, which amplifies a partial segment of the L1 gene (approximately 478 bp), and the amplicon was submitted to direct sequencing. Because nucleotide sequences with satisfactory quality scores were not obtained, the amplicon was cloned and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Cutaneous papillomatosis; BPV; Co-infection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700012

Effect of aubergine (Solanum melongena) on serum and hepatic cholesterol and triglycerides in rats
Provedor de dados: 52
Autores: Silva,Marcelo E; Santos,Rinaldo C; O'Leary,Maureen C; Santos,Ronald S.
The present work reports the effect of aubergine extract on serum and hepatic cholesterol and triglycerides levels in adult rats. Fisher rats were divided into three groups: the first one received a normolipidic diet and water , serving as a control; the other two received a hypercholesterolaemic diet with 30% vegetable oil and 1% cholesterol, one of these being given water while the other was given an aubergine extract. After 28 days the animals were sacrificed and serum and hepatic cholesterol and triglycerides levels were assessed. The obtained results indicated that under the experimental conditions employed, the aubergine extract increased serum and decreased hepatic cholesterol and had little or no effect on both serum and hepatic triglycerides.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aubergine; Eggplant; Solanum melongena; Serum cholesterol; Hepatic cholesterol.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300011

In vitro Anticancer Property of Yellow Pigment fromStreptomyces griseoaurantiacus JUACT 01
Provedor de dados: 52
Autores: Prashanthi,Kuruvalli; Suryan,Sandeep; Varalakshmi,Kilinger Nadumane.
ABSTRACT Despite the complications in isolation of pigments, microbial pigments are increasingly gaining the attention of researchers because of their broad range therapeutic potentials, especially against cancer. In this study the cytotoxic and anti proliferative potentials of yellow pigment from Streptomyces griseoaurantiacus JUACT 01 isolated from soil are investigated. The effect of pigment treatment on the growth and proliferation of in vitro cervical cancer cells (HeLa) and liver cancer cells (Hep G2) was tested by various methods. Significant cytotoxicity was observed with IC 50 values as low as 1.5 and 1.8 µg /mL with HeLa and Hep G2 cells respectively. The pigment exhibited non toxic effects on human lymphocytes. Decrease in the number of viable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticancer activity; ESI-MS; Fluorescence microscopy; Streptomyces griseoaurantiacus JUACT 01; Yellow pigment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600869

Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles
Provedor de dados: 52
Autores: Carneiro,Lara Aparecida Buffoni Campos; Costa-Silva,Tales Alexandre; Souza,Cláudia Regina Fernandes; Bachmann,Luciano; Oliveira,Wanderley Pereira; Said,Suraia.
This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE). The immobilized enzyme retained an activity of about 53.5 % after five reuses, using p-NPP as substrate. Physical adsorption of lipase onto highly deacetylated CHM showed 46.2 % of enzymatic activity and 28.6% of IE. This immobilized derivative did not lose activity up to 80 days of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme immobilization; Chitosan; Cercospora kikuchii; Lipase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400578

Abnormal spindles in second meiosis in canola (Brassica napus and Brassica campestris)
Provedor de dados: 52
Autores: Souza,Alice Maria de; Pagliarini,Maria Suely; Carraro,Ivo Marcos.
Studies were carried out on the occurrence of abnormal spindles in the second meiotic division in some canola cultivars recently introduced in Brazil. Fusion of spindles was observed in metaphase II rejoining the two sets of chromosomes segregated in anaphase I and also sequential and tripolar spindles were discovered rejoining two sets of chromatids segregated in anaphase II. The frequency of cells with abnormal spindles ranged from 3.18 to 8.10%. The results suggested that this abnormality was caused by environmental stress that affected the plants during the blooming period.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canola Brasssica napus; Brassica campestris Abnormal spindles Unreduced gametes.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100007

Sampling Methodology of Alphitobius diaperinus (Coleptera: Tenebrionidae) Population in Poultry Houses
Provedor de dados: 52
Autores: Oliveira,Daian Guilherme Pinto de; Miguel,Rafael Freire; Bonini,Andrea Kusumota; Alves,Luis Francisco Angeli.
Abstract This paper describes a methodology to evaluate Alphitobius diaperinus (Darkling beetle) population in order to estimate the effectiveness of control methods, consisting of direct sample collections from the poultry litter in 18 points, counting of adults and larvae, and subsequent comparison of the values obtained before and after of control strategies. It allows estimating the total population, population in a known area, as well to analyze the variations in different points all over the aviary. The method has shown to be very versatile, being indicated for population studies and efficacy evaluations, once it has already been validated in different experimental conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lesser mealworm; Darkling beetle; Direct sampling.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100414

Cassava Pulp Enzymatic Hydrolysate as a Promising Feedstock for Ethanol Production
Provedor de dados: 52
Autores: Valeriano,Ixthá Hasselmann; Marques,Gabriel Lessa Lisboa; Freitas,Suely Pereira; Couri,Sonia; Penha,Edmar das Mercês; Gonçalves,Márcia Monteiro Machado.
ABSTRACT The aim of this study was to produce bioethanol from enzymatic hydrolysates of cassava pulp, a by-product of cassava flour manufacturing, using an alcohol-tolerant Saccharomyces cerevisiae strain. First, the best operational condition of the starch hydrolysis process was determined through a complete factorial design (24), with triplicates at the central point. The independent variables evaluated were: the concentrations of α-amylase (Termamyl 2X) and glucoamylase (AMG 300L) and both liquefaction and saccharification times. The most favorable hydrolysis condition in the assay was achieved using 0.517 mL AMG.g starch-1 and 0.270 mL Termamyl.g starch-1, with liquefaction and saccharification times of 1 and 2 h, respectively. The broth obtained at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Starch waste; Enzymatic hydrolysis; Alcoholic fermentation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100207

An ordination technique for fish diet comparisons
Provedor de dados: 52
Autores: Penczak,Tadeusz; Agostinho,Angelo A.; Hahn,Norma S.
The frequency of nine food types in the guts of 58 fish species from the Paraná River and its floodplain was compared using similarity definition. A synthetic table ordered on the basis of this definition, with fish species on axis y, and food types on axis x, enabled easy reading numerous kinds of information on feeding ecology of fish in the catchment. For example: 1) which fish species were generalists, specialists or opportunists, 2) those classified as herbivorous species consumed small amounts of animal food, 3) that fish consumed any food type that contained transformed solar energy and matter, 4) what are food preferences dependent on fish taxonomic affinity. The possibility of employing synthetic tables for investigating spatial and temporal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paraná River; Floodplain; Fish; Feeding; Similarity definition; Synthetic table.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100013

Vehicle thermal microclimate evaluation during Brazilian summer broiler transport and the occurrence of PSE (Pale, Soft, Exudative) meat
Provedor de dados: 52
Autores: Simões,Gislaine Silveira; Oba,Alexandre; Matsuo,Tiemi; Rossa,Alessandro; Shimokomaki,Massami; Ida,Elza Iouko.
The formation of a thermal microclimate within the vehicle during the transport of broilers from farm to slaughterhouse affects the birds' welfare and potentially promotes the development of PSE meat. Thus, the aim of this work was to evaluate the effects of routine commercial practices on the vehicle microclimate formed during transportation of broilers. Twenty-four hours postmortem Pectoralis major m. samples were classified as PSE meat by determining pH and color (L*, a* e b*). Results showed that broiler located at the rear of the truck and with longer journey presented higher amounts of PSE meat because birds were under harsh conditions of both temperature and relative humidity. The ventilation decreased gradually from the front to the rear of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal welfare; Poultry meat quality; Thermal stress; Poultry breast meat; Color abnormality.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700025

Using fluid bed granulation to improve the dissolution of poorly water-soluble drugs
Provedor de dados: 52
Autores: Takahashi,Andrea Ikeda; Lourenço,Felipe Rebello; Duque,Marcelo Dutra; Consiglieri,Vladi Olga; Ferraz,Humberto Gomes.
In this study, fluid bed granulation was applied to improve the dissolution of nimodipine and spironolactone, two very poorly water-soluble drugs. Granules were obtained with different amounts of sodium dodecyl sulfate and croscarmellose sodium and then compressed into tablets. The dissolution behavior of the tablets was studied by comparing their dissolution profiles and dissolution efficiency with those obtained from physical mixtures of the drug and excipients subjected to similar conditions. Statistical analysis of the results demonstrated that the fluid bed granulation process improves the dissolution efficiency of both nimodipine and spironolactone tablets. The addition of either the surfactant or the disintegrant employed in the study proved to have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluid bed; Granulation; Dissolution efficiency; Nimodipine; Spironolactone.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300020

Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil
Provedor de dados: 52
Autores: Thomaz-Soccol,Andréa; Mocellin,Marcos; Mulinari,Fabiane; Castro,Edilene Alcântara de; Queiroz-Telles,Flávio de; Alcântara,Fernanda de Souza; Bavaresco,Marcello Tortelli; Hennig,Luciane; Andraus,Anielo; Luz,Ennio; Thomaz-Soccol,Vanete.
The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucocutaneous leishmaniasis; Molecular diagnosis; Leishmania (Viannia) braziliensis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008

Erratum: Blackberry Vinegar Produced By Successive Acetification Cycles: Production, Characterization And Bioactivity Parameters
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300905

Evaluation on the Pathogenesis of Streptococcus agalactiae in Nile Tilapia (Oreochromis niloticus)
Provedor de dados: 52
Autores: Pretto-Giordano,Lucienne Garcia; Müller,Ernst Eckehardt; Freitas,Julio Cesar de; Silva,Vanessa Gomes da.
The pathogenesis of a Streptococcus agalactiae was evaluated in a three-period experiment. Two groups of 40 fishes were intraperitoneally (i.p.) challenged in each experimental period with different infective doses of the pathogen. Doses varied from 1.0 x 10(6) to 1.5 x 10(8) CFU/fish. One group of 40 tilapia i.p. injected with tryptic soy broth (TSB) was used as a control group in each period. Mortalities varied from 67.5% in group 8 (infective dose 1.0 x 10(6) CFU/fish) to 90.0% in group 1 (infective dose 1.5 x 10(8) CFU/fish). Significant differences in mortalities were found only between group 8 and each of the other groups, except group 5 (infective dose 6.0 x 10(6) CFU/fish; mortality 75.0%). The highest mortality coefficients were observed in days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus agalactiae; Nile tilapia (Oreochromis niloticus); Clinical signs; Death rate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100011

Analysis of Production and Consumption of Electric Energy in the Green Office of UTFPR in Curitiba
Provedor de dados: 52
Autores: Krasnhak,Larissa Barbosa; Mello,Elis Almeida Medeiros de; Moura,Guilherme R.; Santos,Francielle da Rocha; Urbanetz Junior,Jair.
ABSTRACT The increasing demand for electricity and the scarcity of resources, require renewable energy sources and efficient equipment that reduce the consumption of electricity. The Green Office (GO) of the Universidade Tecnológica Federal do Paraná (UTFPR) is a sustainable building that uses strategies to reduce impacts to the environment, one of them being the use of the grid connected photovoltaic system (on-grid). The on-grid was installed in 2011 and since then has been feeding the GO and another building (block V) belonging to UTFPR. This article presents a comparison between an estimate of energy consumption and the generation of energy through the on-grid. By means of the estimated consumption, a survey in loco of the scenario of expenditures of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green Office; Photovoltaic Solar Energy; Energy consumption; Energy production.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200233

Current Panorama and 2025 Scenario of Photovoltaic Solar Energy in Brazil
Provedor de dados: 52
Autores: Urbanetz,Isabela Valpecovski; Moura Netto,Allana de; Scolari,Bruno; Leite,Vicente; Urbanetz Junior,Jair.
Abstract The photovoltaic solar energy in Brazil was boosted by Normative Resolution No. 482/2012 of ANEEL, which regulated the micro and mini generation in the compensation system, and by the specific auctions for photovoltaic plants carried out by the federal government. However, the country still has little representation of the solar energy in its electrical matrix, about 0.13%, with approximately 1% of the installed capacity of electric power generators considering all the sources, both values ​​refer to the year 2017. In the initial moments of a technology, its growth occurs irregularly and because there is little installed capacity, any added value can cause abrupt variations in the percentage from one year to another, that is, its growth still does...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic Solar Energy; Energy Scenario; Installed Capacity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200210

Photosynthetic capacity and water use efficiency in sugarcane genotypes subject to water deficit during early growth phase
Provedor de dados: 52
Autores: Silva,Marcelo de Almeida; Jifon,John Lonfover; Santos,Claudiana Moura dos; Jadoski,Cleber Junior; Silva,Jorge Alberto Gonçalves da.
The objective of this study was to compare the gas exchange, photosynthetic capacity and water potential of sugarcane genotypes cultivated under water deficit conditions imposed during the initial growth phase. Experiments were performed in a greenhouse using two sugarcane genotypes namely: HoCP93-776 (drought susceptible) and TCP02-4587 (drought tolerant). Sixty days after planting, two different water treatments were applied (i.e., with or without water deficit). At 0,30 and 60 days after the treatment, gas exchange variables were evaluated for their relationship with water use, intrinsic instantaneous water use efficiency and instantaneous carboxylation efficiency. The SPAD index, photosynthetic pigments, water potential and relative water content in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharum spp.; Photosynthesis; Stomatal conductance; Chlorophyll; Drought tolerance; Water potential.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500004

Whey protein improves HDL/non-HDL ratio and body weight gain in rats subjected to the resistance exercise
Provedor de dados: 52
Autores: Teixeira,Kely Raspante; Silva,Marcelo Eustáquio; Neves,Leandro Xavier; Santos,Rinaldo Cardoso dos; Pedrosa,Maria Lúcia; Haraguchi,Fabiano Kenji.
The aim of this study was to evaluate the effects of resistance exercise, such as weight-lifting (WL) on the biochemical parameters of lipid metabolism and cardiovascular disease risk in the rats fed casein (control) or whey protein (WP) diets. Thirty-two male Fisher rats were randomly assigned to sedentary or exercise-trained groups and were fed control or WP diets. The WL program consisted of inducing the animals to perform the sets of jumps with weights attached to the chest. After seven weeks, arteriovenous blood samples were collected for analysis. The WL or WP ingestion were able to improve the lipid profile, reducing the TC and non-HDL cholesterol concentrations, but only WP treatment significantly increased the serum HDL concentrations, thereby...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dietary protein; Lipid profile; Cholesterol; Cardiovascular disease risk.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600019

Anatomical confirmation of root parasitism in Brazilian Agalinis Raf. species (Scrophulariaceae)
Provedor de dados: 52
Autores: Elias,Samira Ismael; Souza,Vinicius Castro; Appezzato-da-Glória,Beatriz.
Agalinis Raf. consists approximately of 60 species, 14 of which occur in Brazil. The genus presents predominantly american distribution and the Brazilian species appears mainly in high areas of Minas Gerais. The North-American species are refered as hemiparasites, but there is no anatomical data about it in relation to the Brazilian species. Anatomical studies were conducted to verify whether the Agalinis species from Brazil were root parasites or not. The eight species analysed were presented haustoria which varied in shape, arrangement and size. They were generally elliptic or globose structures and mostly were tightly sticked to other roots in a solitary or clustered manner. The seriate sections of haustoria showed that there was a xylem connection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haustorium; Hemiparasitism; Vascular connection; Root host.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300013

Morphological Plasticity and Gas Exchange of Ligustrum lucidumW.T. Aiton in Distinct Light Conditions
Provedor de dados: 52
Autores: Larcher,Letícia; Nogueira,Guilherme; Boeger,Maria Regina.
ABSTRACT The objective of this study was to understand the different morpho-physiological strategies of Ligustrum lucidum, an invasive species occurring in Brazilian forest fragments under heterogeneous light conditions. Ten individuals of L. lucidum were selected and evaluated for morphological of the leaves and physiological traits. For morphological parameters were evaluated: length, width, area, angle, petiole length, dried mass, total thickness, palisade parenchyma and spongy parenchyma thickness, abaxial and adaxial epidermis thickness, stomata density, leaf density and specific leaf area. The physiological traits were vapor-pressure deficit, assimilation rate, CO2 sub-stomata concentration, intrinsic water-use efficiency, transpiration rate and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Invasive plants; Phenotypic integration; Assimilation rates; Glossy privet.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600877

Production of rabbit antibodies against purified Glucose oxidase
Provedor de dados: 52
Autores: Zia,Muhammad Anjum; Ain,Qurat-ul; Iftikhar,Tehreema; Abbas,Rao Zahid; Rahman,Khalil-ur.
Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) resulted into 22.53 U mg-1 specific activity and 10.27 fold purification. This was applied to sephadex G-200 column for gel filtration chromatography. It was observed that enzyme achieved 59.37 U mg-1of specific activity with 27.08 fold purity and 64.36% recovery. Purified glucose oxidase was injected into rabbits through intravenous route, to raise the glucose oxidase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose oxidase; A. niger; Antibodies; Rabbits.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100008

Effect of freezing and processing technologies on the antioxidant capacity of fruit pulp and jelly
Provedor de dados: 52
Autores: Bof,Cristine Maso Jeusti; Fontana,Roselei Claudete; Piemolini-Barreto,Luciani Tatsch; Sandri,Ivana Greice.
The effect of freezing and processing technology on the antioxidant capacity of grape (Vitis vinifera), apple (Malus domestica), strawberry (Fragaria x Anassa), pear (Pyrus communis L.), guava (Psidium guajava L.), and fig (Ficus carica L.) was evaluated for 90 days. Under a storage temperature of -15 º C, there was no significant difference in the antioxidant capacity of grape and fig pulp, and a higher antioxidant capacity was found for guava pulp (27 µmol/g). While the technological processing did not affect the antioxidant capacity of pear and apple jellies, all other jellies studied showed a reduced antioxidant capacity. The processing reduced the antioxidant capacity of grapes in 45%. Among the fruit products, the highest antioxidant activities were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional foods; Pulps; Jellies; Free radicals; Storage.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100014

Improving Aspergillus niger tannase yield by N+ ion beam implantation
Provedor de dados: 52
Autores: Jin,Wei; Nie,Guangjun; Liu,Hui; Xiaoran,Yang; Gong,Guohong; Wang,Li; Zheng,Zhiming.
This work aimed to improve tannase yield of Aspergillus niger through N+ ion beam implantation in submerged fermentation. The energy and dose of N+ ion beam implantation were investigated. The results indicated that an excellent mutant was obtained through nine successive implantations under the conditions of 10 keV and 30-40 (×2.6×10(13)) ions/cm², and its tannase yield reached 38.5 U/mL, which was about five-time higher than the original strain. The study on the genetic stability of the mutant showed that its promising performance in tannase production could be stable. The studies of metal ions and surfactants affecting tannase yield indicated that manganese ions, stannum ions, xylene and SDS contained in the culture medium had positive effects on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; N+ ion implantation; Tannase.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100018

Antimicrobial effect of Anacardium Occidentale extract and cosmetic formulation development
Provedor de dados: 52
Autores: Gonçalves,Gisele Mara Silva; Gobbo,Juliana.
The objective of this work was to assess the activity of the extract of Anacardium occidentale Staphylococcus epidermidis and S. aureus and then to develop cosmetic formulations from those extracts. These formulations were stable in relation to their pH and rheological behavior, but were gradually darkened when stored for assessment at temperatures between 40 and 60ºC. Thus, even though the extract appeared a promising raw material for use in cosmetic formulations, those compounds might still require adjustments to improve the stability.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anacardium occidentale L; Antimicrobial; Cosmetic; Extract; Rheology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600006

Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation
Provedor de dados: 52
Autores: Benjamin,Sailas; Pandey,Ashok.
Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200016

Comparative effect of glucagon and isoproterenol on hepatic glycogenolysis and glycolysis in isolated perfused liver
Provedor de dados: 52
Autores: Vardanega-Peicher,Márcia; Galletto,Ricardo; Pagliarini e Silva,Sarah; Bazotte,Roberto Barbosa.
The effect of glucagon and isoproterenol (beta-adrenergic agonist) on hepatic glycogenolysis and glycolysis in isolated perfused liver was compared. The levels of isoproterenol and glucagon which promoted the maximal activation of glycogenolysis were 20 muM and 1nM respectively. However, glucagon (1 nM) not only increased glycogenolysis but also inhibited glycolysis. Because adenosine-3'-5'-cyclic monophosphate (cAMP) is a common second messenger to glucagon and isoproterenol, the level of cAMP that simulates the effect of these substances were investigated. The concentration of cAMP that inhibited glycolysis was five times higher (15 muM) than that which stimulated glycogenolysis (3 muM). Similar inhibition of glycolysis was obtained with cAMP agonists...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic glycolysis; Hepatic glycogenolysis; Glucagon; Isoproterenol; CAMP.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400010

Limnological characteristics and seasonal changes in density and diversity of the phytoplanktonic community at the Caçó pond, Maranhão State, Brazil
Provedor de dados: 52
Autores: Dellamano-Oliveira,Maria José; Senna,Pedro Américo Cabral; Taniguchi,Glória Massae.
Seasonal changes of the phytoplanktonic community and limnological abiotic characteristics of Caçó pond (Maranhão State, Brazil) was evaluated from two field researches during the rainy (April 1999) and dry (November 1999) seasons. Measurements of twelve chemical and physical variables and phytoplankton collections were carried out at eight sampling stations. The Chlorophyceae and Cyanobacteria groups were in highest fractions during the rainy and dry seasons, respectively. The limnological abiotic variables showed a homogenous spatial distribution. The cluster analysis, using Bray-Curtis distance, distinguished two major groups, represented by the most common and abundant species in both the periods. The results showed that the climate regime, due to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seasonality; Phytoplankton; Diversity; Tropical region.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400019

Extraction optimization of soluble compounds of yerba maté
Provedor de dados: 52
Autores: Sambiassi,César; Escalada,Andrea M.; Schmalko,Miguel E.
The objective of this research was the extraction optimization of water soluble compounds of yerba maté. Measures variables were extract concentration and weight of leaves and twigs. Controlled variables were time and temperature of extraction and water/solid relation. A surface response method of three variables was used as experimental design, with 20 experiences in each case. The range of each variable, defined in the experimental design, was: extraction time, 13.2 to 46.8 minutes; temperature, 48.2 to 81.8°C and water solid relation, 4.64 to 11.36 g water/100 g of dry solid. Extract weight varied from 13.14 to 29.56 g in leaves and 8.98 to 16.32 g in twigs (each one per 100 g of dry solid). Extract concentration varied between 2.17 and 3.43 g/100 ml in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba maté; Soluble compounds; Optimization.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200010

Effect of the aqueous extracts of the seeds of Talisia esculenta and Sapindus saponaria on fall armyworm
Provedor de dados: 52
Autores: Santos,Wander Laizo dos; Freire,Maria das Graças M.; Bogorni,Paulo Cesar; Vendramim,José Djair; Macedo,Maria Lígia R.
The effect of aqueous extracts of Talisia esculenta (T.E.) and Sapindus saponaria (S.S.), were evaluated on the development and mortality of 8-14th day-life Spodoptera frugiperda, an important pest of maize. Corn leaves were dipped in the aqueous extracts (1% w/v) and offered as food to the caterpillars. The treated corn leaves with the extracts caused larval mortality (26.71%/T.E.; 63.3%/S.S.) and also showed effect on the larval weight (237.50 mg/T.E.; 86.65 mg/S.S.) when compared with the control (11.3% and 293.45 mg), mortality and larval weight, respectively. The electrophoresis with gelatin (0.1%) showed two very clear white areas (trypsin activity) in the caterpillars midgut of all the treatments. Only the caterpillar treated with S. saponaria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Talisia esculenta; Sapindus saponaria; Sapindaceae; Spodoptera frugiperda; Natural insecticide.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200018

Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
Provedor de dados: 52
Autores: Michelena,Georgina L.; Martínez,Aidín; Bell,Antonio; Carrera,Emilia; Valencia,Roxana.
Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leuconostoc mesenteroides; Dextransucrase enzyme; Scale-up; Oxygen transfer; Fed batch fermentation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300017

Styrene biofiltration in a trickle-bed reactor
Provedor de dados: 52
Autores: Novak,V.; Paca,J.; Halecky,M.; Soccol,Carlos Ricardo.
The biological treatment of styrene waste gas in a trickle-bed filter (TBF) was investigated. The bioreactor consisted of a two-part glass cylinder (ID 150 mm) filled with 25 mm polypropylene Pall rings serving as packing material. The bed height was 1m. Although the laboratory temperature was maintained at 22 ºC, the water temperature in the trickle-bed filter was slightly lower (about 18 ºC).The main aim of our study was to observe the effect of empty-bed residence time (EBRT) on bioreactor performance at a constant pollutant concentration over an extended time period. The bioreactor was inoculated with a mixed microbial consortium isolated from a styrene-degrading biofilter that had been running for the previous two years. After three weeks of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofiltration; Styrene; Trickle - bed filter; Loading by air flow rate.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200019

Physico-chemical quality changes in mangaba (hancornia speciosa gomes) fruit stored at different temperatures
Provedor de dados: 52
Autores: Carnelossi,Marcelo Augusto Gutierrez; Sena,Hildo Costa de; Narain,Narendra; Yaguiu,Paula; Silva,Gabriel Francisco da.
The physical-chemical quality changes in mangaba fruit were studied. The fruit which attained full development at half-ripe stage were harvested and initially stored at 6, 8, 10 and 12±1ºC for four days. After this period, the fruit were transferred to an acclimatized room (24±2ºC) and maintained for five days. For control purposes, recently harvested fruit were stored directly in an acclimatized room (24±2ºC) for six days. After the transfer and storage at 24ºC, fruit were analyzed daily for their vitamin-C, soluble solids (ºBrix), titratable acidity, pH and firmness contents. In fruit directly stored at 24ºC, there was a sharp fall in vitamin C and acid contents. The fruit firmness decrease, after four days of storage, and they turned totally ripe. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cold storage; Postharvest; Tropical fruits; Vitamin C.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400023

Production of grape juice powder obtained by freeze-drying after concentration by reverse osmosis
Provedor de dados: 52
Autores: Gurak,Poliana Deyse; Cabral,Lourdes Maria Correa; Rocha-Leão,Maria Helena.
This study aimed to evaluate the freeze-drying process for obtaining grape juice powder by reverse osmosis using 50% grape juice pre-concentrated (28.5 °Brix) and 50% hydrocolloids (37.5% maltodextrin and 12.5% arabic gum). The morphology of the glassy food showed the absence of crystalline structure, which was the amorphous wall that protected the contents of the powder. The samples were stored in clear and dark containers at room temperature, evaluated for their physical (X-ray diffraction) for 65 days and chemical (polyphenol content) stability for 120 days. During the storage time in plastic vessels, samples remained physically stable (amorphous) and the phenolic concentration was constant, indicating the potentiality of this technique to obtain a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic compounds; Maltodextrin; Arabic gum; X-ray diffraction; Scanning electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600017

Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair
Provedor de dados: 52
Autores: Maia Filho,Antonio Luiz Martins; Amaral,Fabrício Pires de Moura do; Martin,Airton Abrahão; Soares,Luís Eduardo Silva.
The aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Three experimental groups were tested as Group C - control, surgery procedure without cerami c implant, Group Ce - uncoated Al 2O3 implants (n = 6) and Group CeHA - apatite-coated Al2O3 implants (n = 6). The mineralization of the Al2O3 implants was analyzed in bone fragments using FT-Raman spectroscopy. Raman peaks at 959 cm-1 and 2940 cm-1 evaluated the inorganic and organic bone content, respectively. In vivo citotoxicity was analyzed using micronucleus test....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone regeneration; Alumina foams; Apatite coating; FT-Raman spectroscopy; Micronucleus test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300004

Inheritance and linkage relationships of allozyme variants of ilex paraguariensis St. Hil
Provedor de dados: 52
Autores: Wendt,Simone Neumann; Sousa,Valderês Aparecida de; Sebbenn,Alexandre Magno; Sturion,José Alfredo; Luíz,Felipe; Santos,Elisa Caroline da Silva; Quoirin,Marguerite.
Eighteen enzyme systems were examined in Ilex paraguariensis St. Hil. using starch gel electrophoresis. Seven out of 12 active isozyme systems revealed one or more polymorphic loci (PGI, GOT, MR, G-6PDH, MDH, NDH, and 6-PGDH). However, the segregation and linkage analyses were performed only for PGI, GOT, G-6PDH and 6-PGDH systems. Gene segregation at these loci was regular, except for a few trees that showed segregation distortion. Weak linkage disequilibrium between loci was detected, but it was not enough to influence the multilocus estimate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erva-mate (matê); Electrophoresis; Loci; Allele; Segregation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600016

Effect of Organic Matter on Manganese Solubility
Provedor de dados: 52
Autores: Andrade,Emilene; Miyazawa,Mário; Pavan,Marcos A.; Oliveira,Edson L. de.
The objective of this study was to re-evaluate some aspects of the relative importance of organic matter on Mn solubility in acid soil conditions. Field study showed that black oats, oil seed radish, elephant grass, lupin, leucaena, and coffee leaves serving as mulch decreased Mn solubility as compared with bare soil. The decrease in Mn solubility with plant mulch was related to increase in soil moisture content. Laboratory study showed that increasing temperature from 25 to 100ºC increased Mn solubility and total soil organic carbon was little changed; from 150 to 200ºC increased both Mn solubility and organic carbon oxidation, and up to 300ºC decreased Mn solubility and stoped organic carbon oxidation . Aluminum solubility always increased with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant residue; Mulch; Organic carbon; Heavy metal; Micronutrient.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100003

Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk
Provedor de dados: 52
Autores: Kaur,Hardeep; Kumar,Sachin; Verma,Neelam.
The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 -...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosensor; Urease; Hydrosol- gel immobilization; Lead; Milk; Masking agent.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613

Vertical and horizontal distribution of pollination systems in cerrado fragments of central Brazil
Provedor de dados: 52
Autores: Martins,Fernanda Quintas; Batalha,Marco Antônio.
In fragments of the cerrado, we determined the frequency of pollination systems and analyzed their spatial distribution. We placed 38 transects, sampling 2,280 individuals and 121 species. As expected in Neotropical regions, bee-pollination was the most frequent pollination system. We found a decrease in the frequency of plants pollinated by beetles towards the fragment interior. Similarly, we found significant variation in relation to height just for the bats; there was an increase in the frequency of plants pollinated by bats towards the higher heights. In general, we found no horizontal and vertical variation in the pollination systems, probably as consequence of the more open physiognomy of the cerrado vegetation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central-western Brazil; Cerrado; Pollination; Savanna; Spatial distribution; Woody component.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300016

Identification of Suitable Reference Genes for Gene Expression Normalization in Jatropha curcas L During Development and Under Stress Conditions Using Real Time Quantitative PCR
Provedor de dados: 52
Autores: Rocha,Antonio José; Maranhão,Paulo Abraão; Silva,Rafaela Oliveira; Pohl,Simone; Fonteles,Cristiane S.R.
Jatropha curcas L represent a potential source of raw material for the production of biodiesel. The aim this study was to find potential candidate reference genes in J. curcas tissues. Three softwares were utilized to verify which would be the most stable reference genes in qPCR assay: GeNorm, NormFinder and BestKeeper. The most stable reference genes in developing J. curcas seeds suggested by GeNorm were GAPDH, UCP, actin. However, the best combinations of stable genes in each tissue were identified separately under stress conditions: EF1-α, PP2A2 and GAPDH in total stress, however, in SA stress, four genes were required for normalization: PP2A2, EF1-α, GAPDH and PUB. In PEG stress, four genes also were required: PP2A2, EF1-α, GAPDH and PUB, while in NaCl...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; RT-qPCR; Reference genes; Jatropha curcas L.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100404

Suspended matter and nutrient gradients of a small-scale river plume in Sepetiba Bay, SE-Brazil
Provedor de dados: 52
Autores: Rodrigues,Raphael Paiva; Knoppers,Bastiaan Adriaan; Souza,Weber Friederichs Landim de; Santos,Elisamara Sabadini.
Coastal river plumes represent one of the final stages of material transport across the land-sea interface. Most studies, however have focused on the behavior of medium to large sized river plumes of coastal-shelf waters, whereas small sized river plumes acting within estuaries have been neglected. This study addressed the behavior of suspended particulate matter (SPM), dissolved inorganic nutrients (DIN, DIP and DSi) and Chlorophyll a (Chl. a) of a small sized river plume derived from the closely lain São Francisco and Guandú river channels, set in the Sepetiba Bay estuary, SE-Brazil. Two surface water sampling campaigns were conducted, one in January 2003 (humid summer conditions) and the other in June 2003 (dry winter conditions). On both occasions, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suspended matter; Nutrients; Estuarine gradients; River plume; Sepetiba Bay; SE-Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200030

Fetal Cerebrospinal Fluid Promotes Proliferation and Neural Differentiation of Stromal Mesenchymal Stem Cells Derived from Bone Marrow
Provedor de dados: 52
Autores: Shokohi,Rozmehr; Nabiuni,Mohammad; Moghaddam,Parisa; Irian,Saeed; Miyan,Jaleel A.
ABSTRACT Embryonic cerebrospinal fluid (E-CSF) contains many neurotrophic and growth factors, acts as a growth medium for cortical progenitors, and can modulate proliferation and differentiation of neural stem cells. Mesenchymal stem cells (MSCs) are multipotential stem cells that can differentiate into several types of mesenchymal cells as well as nonmesenchymal cells, such as neural cells. In the present study, the effect of E-CSF on proliferation and neural differentiation of bone marrow mesenchymal stem cells (BM-MSCs) was investigated to test whether E-CSF is capable of driving these cells down the neuronal line. To verify the multipotential characteristics of BM-MSCs, the cells were analyzed for their osteogenic and adipogenic potential. Expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebrospinal fluid; Neural differentiation; Proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100441

Relative growth and sexual maturity of the stone crab Menippe nodifrons Stimpson, 1859 (Brachyura, Xanthoidea) in southeastern Brazil
Provedor de dados: 52
Autores: Bertini,Giovana; Braga,Adriane Araújo; Fransozo,Adilson; Corrêa,Michéle de Oliveira Dias Alves; Freire,Fulvio Aurélio de Morais.
The relative growth and size at onset of morphological sexual maturity of the stone crab Menippe nodifrons were investigated. A total of 399 crabs was captured on Praia Grande and Tenório beaches at Ubatuba. Carapace width (CW) and length, cheliped propodus length and height, abdomen width in females, and gonopod length in males were recorded. In females, the abdominal width showed negative allometry for juveniles and positive allometry for adults; the puberty molt occurred at 31.6 mm CW. In males, the size at onset of morphological sexual maturity was estimated as 29.7 mm CW; the gonopod growth showed positive allometry for juveniles, and an isometric relationship for adults. The gonopod length and the abdominal width were the most appropriate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Sexual Maturity; Menippe; Xanthoidea.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200011

Uncertainty of the density of moist air: Gum x Monte Carlo
Provedor de dados: 52
Autores: Canaves Junior,Moacyr; Pompéia,Pedro José.
In this study two methods of evaluation of uncertainty, the law of propagation of uncertainties (as recommended by GUM) and the method of Monte Carlo, are compared. As a particular case the determination of the density of moist air was considered The results show that there are some differences between both methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Density of moist air; Propagation of uncertainties; Monte Carlo.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200014

Novel Image Classification technique using Particle Filter Framework optimised by Multikernel Sparse Representation
Provedor de dados: 52
Autores: N. R,Bhuvaneswari; V.G,Sivakumar.
ABSTRACT The robustness and speed of image classification is still a challenging task in satellite image processing. This paper introduces a novel image classification technique that uses the particle filter framework (PFF)-based optimisation technique for satellite image classification. The framework uses a template-matching algorithm, comprising fast marching algorithm (FMA) and level set method (LSM)-based segmentation which assists in creating the initial templates for comparison with other test images. The created templates are trained and used as inputs for the optimisation. The optimisation technique used in this proposed work is multikernel sparse representation (MKSR). The combined execution of FMA, LSM, PFF and MKSR approaches has resulted in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multikernel Sparse Representation; Image Classification; Sparse Learning; Level Set Method; Particle Filter Framework; Remote Sensing.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300606

Optimization of drying process of Zea Mays malt to use as alternative source of amylolytics enzymes
Provedor de dados: 52
Autores: Biazus,Joana Paula Menezes; Souza,Anderson Gomes; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambougi,Elias Basile.
This work aimed to study the drying process optimization of maize (Zea Mays) malt for obtaining maize malt, without affecting enzymatic activity of alpha e beta-amylases from maize malt. Results showed that dryer operation must occur in zone at 54°C and 5.18-6 h process time. The maize malt obtained had good enzymatic properties.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha and beta -amylases enzymes; Maize (Zea mays) malt/drying process; Optimization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400023

Attack behavior of Podisus rostralis (Heteroptera: Pentatomidade) adults on caterpillars of Bombyx mori (Lepidoptera: Bombycidae)
Provedor de dados: 52
Autores: Lemos,Walkymário Paulo; Zanuncio,José Cola; Serrão,José Eduardo.
Attack behavior of the predator Podisus rostralis (Stäl) (Heteroptera: Pentatomidae) adults on fourth instar Bombyx mori L. (Lepidoptera: Bombycidae) caterpillars was studied in laboratory conditions. Ten 24 hours old adults of this predator were observed during two hours with the following attack behavior: (1) Predator: prey finding; prey observation; touching prey with antenna; attack behavior; prey paralysis; predator retreat after attack; attack cessation; successive attacks; and (2) Prey: defense. The predator P. rostralis found its prey before attacking and it approached it with slow circular movements. The attack was usually made in the posterior part of the prey to reduce defense reaction. Larger size of prey in relation to the predator resulted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asopinae; Alternative prey; Biological control; Ethology; Insecta.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800014

Lactobacilli isolated from Algerian goat's milk as adjunct culture in dairy products
Provedor de dados: 52
Autores: Marroki,Ahmed; Bousmaha-Marroki,Leila.
In this study, nineteen Lactobacillus isolated from Algerian goat's milk, 13 belonging to L. plantarum, three to L. pentosus, two to L. rhamnosus and one to L. fermentum, were examined in vitro in order to be used as adjunct culture in dairy products. The strains were tested for their proteolytic activity, sensory and safety properties. Strains LbMS16 and LbMS21 L. plantarum and LbMF25 L. rhamnosus presented the highest proteolytic activity. All the tested lactobacilli were able to grow on MRS agar containing 0.5 and 1% (W/V) of oxgall, whereas none produced biogenic amine (BA) from the four tested amino acids and were resistant to pH 2.0 and 3.0, but some strains were able to grow at pH 3.5. None of examined strains were β-haemolytic when grown in hors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Goat's milk; Safety aspect; Adjunct culture; Sensory properties; Antibiotic resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300015

O gênero Erythroxylum P. Browne (Erythroxylaceae) do estado do Paraná, Brasil
Provedor de dados: 52
Autores: Mendonça,Joalice de Oliveira; Cervi,Armando Carlos; Guimarães,Olavo Araújo.
An inventory of the Erythroxylum's species from the State of Paraná, Brazil, was done and the following species were found: E. ambiguum Peyr., E. amplifolium (Mart.) O.E. Schulz, E. anguifugum Mart., E. argentinum O.E. Schulz, E. buxus Peyr., E. campestre A. St.-Hil., E. cuneifolium (Mart.) O.E. Schulz, E. cuspidifolium Mart., E. deciduum A. St.-Hil., E. gonocladum (Mart.) O.E. Schulz, E. nanum A. St.-Hil., E. pelleterianum A. St.-Hil., E. microphyllum A. St.-Hil., E. myrsinites Mart. and E. suberosum A. St.-Hil.. All of then are taxonomically revised and a new synonym is presented: E. cuneifolium (Mart.) O.E. Schulz var. squarrosum O.E. Schulz as synonym of E. cuneifolium (Mart.) O.E. Schulz. Is also discussed the validity of the genus name Erythroxylon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythroxylaceae; Erythroxylum; Flora; Paraná; Taxonomy.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300013

Dye decolorizing potential of a novel fungus Coriolus versicolor ML04 in the medium optimized by response surface methodology
Provedor de dados: 52
Autores: Venil,Chidambaram Kulandaisamy; Lakshmanaperumalsamy,Perumalsamy.
The potential of the white rot fungus, Coriolus versicolor ML04 to decolorize the widely used textile dye Blue BB was tested by employing statistical optimization. Response surface methodology (RSM) involving a central composite design (CCD) was applied to evaluate the interactive effects of four significant factors in different ranges i.e.; glucose (0.5 - 2.5 g/L), yeast extract (0.4 -1.2 g/L), dye concentration (100 - 500 ppm) and inoculum size (5 - 20 % v/v) to decolorize the Blue BB. The results demonstrated the effectiveness of the statistical experimental design and the ability of C. versicolor ML04 for maximum dye decolorization (&gt;96%) at the optimum conditions of the significant factors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blue BB; Coriolus versicolor ML04; Plackett - Burman design; Central composite design.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600028

Characterization and Cytotoxic Evaluation of Silver and Gold Nanoparticles Produced with Aqueous Extract of Lavandula dentata L. in Relation to K-562 Cell Line
Provedor de dados: 52
Autores: Justus,Barbara; Arana,Andrés Fernando Montenegro; Gonçalves,Melissa Marques; Wohnrath,Karen; Boscardin,Patrícia Mathias Döll; Kanunfre,Carla Cristine; Budel,Jane Manfron; Farago,Paulo Vitor; Paula,Josiane de Fátima Padilha de.
Abstract Metallic nanoparticles have great potential as a chemotherapeutic agent. The aim of this study was to develop and characterize silver and gold nanoparticles using a simple method, as well as evaluating the potential cytotoxic activity in relation to the K-562 cell line. For the synthesis, a solution containing the metallic ions was subjected to magnetic stirring with the aqueous extract of Lavandula dentata L. and a change of colour was observed. With the data obtained from the analyses we concluded that the nanoparticles were successfully obtained by a simple and green method using the aqueous extract of L. dentata. The obtained nanoparticles presented a reduced size, a low level of polydispersion, and a homogenous spherical shape. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green synthesis; Metallic nanoparticles; Myelogenous leukemia line; Cytotoxic potential; Lamiaceae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100420

Relationship between seminal malondialdehyde levels and sperm quality in fertile and infertile men
Provedor de dados: 52
Autores: Colagar,Abasalt Hosseinzadeh; Pouramir,Mehdi; Marzony,Eisa Tahmasbpour; Jorsaraei,Sayed Gholam Ali.
The aim of this study was to determine the level of malondialdehyde in seminal plasma of fertile and infertile men and investigate its relationship with sperm quality. Results showed that the mean of ± S.D. MDA concentration in seminal plasma of infertile men (0.94 ± 0.28 nmol/ml) was significantly higher than fertile men (0.65 ± 0.17 nmol/ml) (p value&lt; 0.001), and had negative relationship with sperm count, motility and morphology. Therefore it could be concluded that increase in lipid peroxidation was associated with sperm membrane destructed and high level of MDA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seminal plasma; Male infertility; Sperm quality; Malondialdehyde (MDA); Lipid peroxidation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600010

Evaluation of the average performance of topcrosses among food and grain type soybean
Provedor de dados: 52
Autores: Yokomizo,Gilberto K.; Vello,Natal A.
The food type soybean (exotic) are, in general, non-adapted to the Brazilian growing conditions. A breeding strategy to develop adapted food type soybean should involve crossing exotic with the available grain type genotypes. We evaluated the performance of topcrosses among food type and grain type soybeans. Best progenies showed different performance rates in each environment and they were not always the same in the different environments and joint analysis of lodging (L), plant height at maturity (PHM) and agronomic value (AV) was essential to avoid selection of plants with good performance for one trait and bad for another.) Piracicaba autumn environment (PA) caused low individual plant yield performance (IPY), and consequently, cultivation was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Food type soybean; Edamame; Topcross; Vegetal breeding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300003

Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss
Provedor de dados: 52
Autores: Ninhaus-Silveira,Alexandre; Foresti,Fausto; Tabata,Yara Aiko; Rigolino,Marcos Guilherme; Veríssimo-Silveira,Rosicleire.
Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oncorhynchus mykiss; Cryopreservation; Sex reversal; Gynogenesis; Semen.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009

A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee
Provedor de dados: 52
Autores: Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020

Fish silage in black bass (Micropterus Salmoides) feed as an alternative to fish meal
Provedor de dados: 52
Autores: Arruda,Lia Ferraz de; Borghesi,Ricardo; Portz,Leandro; Cyrino,José Eurico Possebon; Oetterer,Marília.
The objective of this study was to use the residues of fermented sardine to elaborate the acid fish silage and its use in feed for aquaculture. Biological assay was performed by feeding largemouth bass (Micropterus salmoides) fingerlings (initial weight 22g), with extruded diets (41% crude protein; 3,600 kcal/kg digestible energy) containing 0.0, 7.5, 10.0, 12.5, or 15.0% of fish silage in partial substitution to the fish meal. The feed conversion ratio and weight gain for the treatments were: 1.26 and 15.76g; 1.11 and 17.07g; 1.19 and 17.81g; 1.18 and 19.83g; 1.47 and 14.64g, respectively. No significant differences (P&lt;0.05) were detected among the treatments. Results indicated that it was possible to use up to 15% of acid fish silage as partial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Residue; Carnivorous fish; Protein; Fish nutrition.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500025

Selection of an experimental fish ladder located at the dam of the Itaipu Binacional, Paraná River, Brazil
Provedor de dados: 52
Autores: Fernandez,Domingo Rodriguez; Agostinho,Angelo Antônio; Bini,Luis Mauricio.
The specific selection of a weir and orifice type experimental fish ladder in the dam of the Itaipu Reservoir (Paraná River) was evaluated by samplings in the river downstream and at two points along the ladder (at heights of 10 m and 27 m) during 28 months. Among the 65 species recorded in the river (immediate downstream of the dam), 27 were captured on the ladder. The species that showed highest density on the ladder, the majority migratory, were moderately, or only slightly, abundant downstream. Among the most abundant species downstream, only one, non-migratory, was recorded in the ladder. The structure presented a negative selection in relation to large migratory pimelodids that might be overcome by enlarging the scale of its design. The sampling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish ladder; Fish migration; Selectivity; Fish transposition; Reservoir.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400012

Cichorium intybus from India: GC-MS Profiling, Phenolic Content and in vitro Antioxidant Capacity of Sequential Soxhlet Extracted Roasted Roots
Provedor de dados: 52
Autores: Singh,Ravinder; Chahal,Khushminder Kaur.
Abstract Research on the bio-activities and chemical composition of roasted C. intybus roots from India is very little. In present studies GC-MS analysis of volatile components of roasted C. intybus roots, phenolics and flavonoid content estimation and antioxidant potential of roasted C. intybus roots was carried out. Antioxidant potential was also evaluated using FRAP, DPPH, hydroxyl radical, nitric oxide and superoxide free radical scavenging method. Extracts were prepared by sequential Soxhlet extraction. GC-MS analysis of volatile components of roasted C. intybus root extracts revealed that 5-hydroxymethyl furfural was major volatile component in dichloromethane and methanol extract whereas lupeol and its derivative compounds were major constituents of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant potential; Chicory; Chemical Composition; Solvent extracts.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100419

Relationships among oil content, protein content and seed size in soybeans
Provedor de dados: 52
Autores: Marega Filho,Mario; Destro,Deonisio; Miranda,Lilian Azevedo; Spinosa,Wilma Aparecida; Carrão-Panizzi,Mercedes Concórdia; Montalván,Ricardo.
During 1995/96 and 1996/97, experiments were carried out at Londrina State University, aiming at quantifying the oil and protein contents in two groups of soybean genotypes; estimating the phenotypic, genotypic and environmental correlations existent among oil, protein content and seed size, and identifying genotypes for direct human consumption with high protein content. The evaluated characters were Weight of a Hundred Seeds (WHS), expressed in grams/100 seeds, Oil Content (OC) and Protein Content (PC), expressed in %. In the experiment carried out in the field, OC ranged from 12 to 20.37 % and PC from 35.66 to 41.75% while in the experiment carried out in the greenhouse OC ranged from 12.26 to 21.79 % and PC from 32.95 to 41.56 % . The correlations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Glycine max; Seed composition; Seed size; Oil content; Protein content.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100004

Genetic Diversity of Some Capparis L. Species Growing in Syria
Provedor de dados: 52
Autores: Al- Safadi,Bassam; Faouri,Hussam; Elias,Rana.
This work investigated the genetic diversity and relationships among Capparis species growing in Syria using IRAP and ISSR techniques. Forty-seven samples of three Capparis species genotypes were collected from 21 different locations in Syria. The genotypes were morphologically identified based on the descriptions available in the literature. When IRAP technique was used, an average of 71.5% of the amplified fragments were polymorphic compared to 82.04% in ISSR. Morphological characterization along with the cluster and PCoA analyses of the data divided the studied genotypes into three groups. The groups included genotypes identified as Capparis spinosa L, C. sicula Duh., and C. aegyptia Lam. Based on the morphological description, molecular studies and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capparis; Diversity; ISSR; IRAP; Syria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600916

Rust resistance in Arabic Coffee cultivars in northern Paraná
Provedor de dados: 52
Autores: Del Grossi,Leandro; Sera,Tumoru; Sera,Gustavo Hiroshi; Fonseca,Inês Cristina de Batista; Ito,Dhalton Shiguer; Shigueoka,Luciana Harumi; Andreazi,Elder; Carvalho,Filipe Gimenez.
The objective of the study was to evaluate the resistance to rust in coffee cultivars developed by research institutes of Brazil in Paraná state. Resistance to the local leaf rust races was assessed in high disease intensity field conditions at Londrina and Congonhinhas in 2009 and 2010.The cultivars were developed by the EPAMIG/UFV, IAPAR, IAC and MAPA/Procafé. The resistant standard 'IAPAR 59' and the susceptible standards Catuaí Vermelho IAC 144' and 'Bourbon Amarelo' were used. A randomized block design with three replications and plots with 10 plants was used. A scale from 1 to 5 based on the rust intensity was used to evaluate the resistance. The Catiguá MG 1, Catiguá MG 2, IAPAR 59, IPR 98, IPR 104, Palma II, Paraíso H-419-10-6-2-5-1, Paraíso...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Coffea; Hemileia vastatrix; Híbrido de Timor; SH genes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100004

Water type and irrigation time effects on microbial metabolism of a soil cultivated with Bermuda-grass Tifton 85
Provedor de dados: 52
Autores: Nogueira,Sandra Furlan; Paula,Alessandra Monteiro de; Pereira,Bruno Fernando Faria; Salemi,Luiz Felippe; Fonseca,Adriel Ferreira da; Montes,Célia Regina; Victoria,Reynaldo Luiz.
This study investigated the microbial metabolism in Bermuda-grass Tifton 85 areas after potable-water and effluent irrigation treatments. The experiment was carried out in Lins/SP with samples taken in the rainy and dry seasons (2006) after one year and three years of irrigation management, and set up on an entirely randomized block design with four treatments: C (control, without irrigation or fertilization), PW (potable water + 520 kg of N ha-1 year-1); TE3 and TE0 (treated effluent + 520 kg of N ha-1 year-1) for three years and one year, respectively. The parameters determined were: microbial biomass carbon, microbial activity, and metabolic quotient. Irrigation with wastewater after three years indicated no alteration in soil quality for C and ET3; for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reuse; Irrigated agriculture; Microbial respiration; Cmic; QCO2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300007

Diet of a catfish before and after damming of the salto caxias reservoir, iguaçu river
Provedor de dados: 52
Autores: Delariva,Rosilene Luciana; Hahn,Norma Segatti; Gomes,Luiz Carlos.
This study evaluated the diet of Pimelodus sp., before and after damming of Salto Caxias, (waterfall in the Iguaçu river), as well spatial and ontogenetic diet shift. Surveys were carried out from March 1997 to February 1998 (before damming) and from April 1999 to January 2000 (after damming) inside the reservoir and adjacent tributaries. Stomach contents analysis of 682 specimens revealed omnivory with high degree of trophic opportunism to this species. Analysis of variance (three way ANOVA) for scores produced from Detrended Correspondence Analysis (DCA) yielded a significant interaction between the damming phases and size of the individuals (juveniles and adults) (F = 4.68; P = 0.04). After damming, adults exploited mainly fish, whereas the juveniles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catfish; Diet; Trophic opportunism; Reservoir.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500004

Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
Provedor de dados: 52
Autores: Jiang,Yina; Hu,Yanlin; Wang,Biao; Wu,Tianlong.
In this work, a bivalent RNA interference (RNAi) plant-transformation vector was constructed to silence both the flavanone 3-hydroxylase (F3H) gene and the flavone synthase II (GmFNSII) gene in soybean (Glycine max). Two further unit RNAi vectors were constructed for each of these two genes. RNAi-mediated suppression of these genes effectively regulated flavone and isoflavone production in hairy roots that arose from soybean cotyledons transformed with Agrobacterium rhizogenes ATCC15834. Notably, the bivalent RNAi vector had a significantly higher effect for increasing isoflavone production compared with the two unit RNAi vectors. The study highlighted molecular methods that could be used to enhance isoflavone production in soybean and demonstrated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bivalent RNA interference; Flavanone 3-hydroxylase; Flavone synthase II; Isoflavone biosynthesis; Soybean.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200002

Problems and pitfalls in vulvar and cervical cancer sentinel node scintigraphy
Provedor de dados: 52
Autores: Sinzinger,Helmut; Granegger,Susanne; Palumbo,Barbara; Palumbo,Renato.
After the introduction for penile cancer, the sentinel lymph node imaging is increasingly applied in various types of cancer. After the initial learning phase, 105 patients with vulvar and 24 with cervical cancer have been investigated. In vulvar cancer all the imaged sentinel nodes were discovered by the portable probe intraoperatively. No false negative sentinel node was observed. The most critical issue is the tracer application. Performed strictly intradermally, the sentinel node shows up immediately. Concomitant use of isosulfan blue dye did not improve the results and was stopped therefore. Similarly, more superficial (intra/subendothelial) application brings up better results as compared to deeper injection in cervical cancer patients. No false...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sentinel lymph node; Vulvar cancer; Cervical cancer; Scintigraphy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700007

Generalized lineal models for the analysis of binary data from propagation experiments of Brazilian orchids
Provedor de dados: 52
Autores: Mora,Freddy; Gonçalves,Letícia de Menezes; Scapim,Carlos Alberto; Martins,Elias Nunes; Machado,Maria de Fátima Pires da Silva.
This study aimed at applying the generalized linear models (GLM) for the analysis of a germination experiment of Cattleya bicolor in which the response variable was binary. The purpose of this experiment was to assess the effects of the storage temperatures and culture mediums on the seed viability. The analyses of variance was also carried out either with or without the data transformation. All the statistical approaches indicated the importance of the storage temperature on the seed viability. But, the culture media and interaction effects were significant only by the GLM. Based on the GLM, the seeds stored at 10°C increased viability, in which the coconut medium achieved the best performance. The results emphasized the importance of adopting the GLM to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattleya bicolor; Deviance; In vitro culture; Logit model; Maximum-likelihood.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500013

Pentachlorophenol removal by Pleurotus pulmonarius in submerged cultures
Provedor de dados: 52
Autores: Souza,Daniela Farani de; Costa,Silvio Cláudio da; Dacome,Antonio Sérgio; Souza,Cristina Giatti Marques de; Bracht,Adelar; Peralta,Rosane Marina.
Pentachlorophenol (PCP) removal by Pleurotus pulmonarius grown in submerged cultures in the presence and absence of laccase inducers was studied in this work. When PCP was added to a final concentration of 25 mg·L-1 in submerged cultures actively producing laccase, 70% of the PCP was removed after 96 h. The removal of PCP was less than 20% in the cultures with low laccase activity. The results suggested that laccase played an important role in the biodegradation of PCP by P. pulmonarius and that for bioremediation purposes the fungus must be cultured under the conditions of active laccase production.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laccase; Pentachlorophenol; Pleurotus pulmonarius; Xenobiotics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200018

Viability Assays of PLLA Fibrous Membranes Produced by Rotary Jet Spinning for Application in Tissue Engineering
Provedor de dados: 52
Autores: Ciocca,Bárbara Etruri; Munhoz,André Luiz Jardini; Cardoso,Guinea Brasil Camargo; Rodigues,Ana Amélia; Pattaro,Ana Flávia; Kaasi,Andreas; Maciel Filho,Rubens.
Abstract Tissue engineering suggests different forms to reconstruct tissues and organs. One of the ways is through the use of polymeric biomaterials such as poly(L-lactic acid) (PLLA). PLLA is a recognized material in tissue engineering due to its characteristics as biocompatibility and bioresorbability. In this work PLLA fibrous membranes were produced by a simple technique known as rotary jet spinning. The rotary jet spinning consists of fibrous membranes production, with fibers of scale nano/micrometric, from a polymeric solution through the centrifugal force generated by the equipment. The membranes formed were subjected to preliminary in vitro assays to verify the cytotoxicity of the membranes made in contact with the cells. Direct cytotoxicity assays...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue engineering; Poly(L-lactic acid); Biomaterial; Rotary jet spinning; In vitro assay.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100604

Effect of Light Intensity on Initial Survival of Fat Snook (Centropomus parallelus, Pisces: Centropomidae) Larvae
Provedor de dados: 52
Autores: Cerqueira,Vinicius Ronzani; Brügger,André Macedo.
Different light intensities were tested during the larval rearing of fat snook (Centropomus parallelus) to evaluate its influence on survival and functional swim bladder rate. Newly-hatched larvae were obtained by stripping of hormonally induced broodstock, and were stocked in 32-liter tanks at densities from 30 to 50 L-1. Four experiments were carried out testing six light intensities: 50, 100, 200, 500, 1500, and 2500 lx, and total darkness as a control. Mean survival ranged from 0.06 to 16.0% and were significantly influenced by light intensity. Complete darkness resulted in 100% mortality after one week, larvae appeared to have starved since they had empty stomachs. The species has the characteristics of a visual-feeder. Survival was the highest at 200...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat snook; Larva; Centropomus parallelus; Light intensity; First-feeding.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400004

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova
Provedor de dados: 52
Autores: Takahachi,Gisele; Maluf,Márcia Luzia Ferrarezi; Svidzinski,Terezinha Inez Estivalet; Akimoto-Günther,Luciene Setsuko; Hübler,Márcia Rosangela Neves de Oliveira; Bersani-Amado,Ciomar Aparecida; Cuman,Roberto Kenji Nakamura.
The objective of this work was to evaluate biochemical parameters in Paracoccidioides brasiliensis infected mice and the effect of Canova medicine on these parameters. Mice infected with the isolate Paracoccidioides brasiliensis Pb18 and treated with Canova for 17 weeks were used. The biochemical parameters analysed were the levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and amylase, and the concentration of total proteins, albumin and globulins. The results suggested that the animals that were treated with Canova had less functional alterations in their internal organs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Biochemical profile; Canova; Paracoccidioides brasiliensis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700006

Potential Use of Polysaccharides from the Brown Alga Undaria pinnatifida as Anticoagulants
Provedor de dados: 52
Autores: Faggio,Caterina; Morabito,Marina; Minicante,Simona Armeli; Piano,Giada Lo; Pagano,Maria; Genovese,Giuseppa.
Undaria pinnatifida (U. pinnatifida) is a highly invasive species and has caused concern all over the world because it has invaded coastal environments, has the potential to displace native species, significantly alters habitat for associated fauna, and disturbs navigation. Any attempt to eradicate it would be futile, owing to the elusive, microscopic gametophyte, and because the alga thrives in sites rich in anthropic activities. Venice Lagoon is the largest Mediterranean transitional environment and the spot of the highest introduction of non-indigenous species, including U. pinnatifida, which is removed as a waste. We demonstrated that polysaccharide extracts from U. pinnatifida have an anticoagulant effect on human blood in vitro and are not cytotoxic....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algal extract; Anticoagulant activity; Cytotoxicity; Fucoidans; Human blood cells; Undaria pinnatifida.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500798

Effects of gamma radiation on the stability and degradation kinetics of phenolic compounds and antioxidant activity during storage of (Oryza sativa L.) black rice flour
Provedor de dados: 52
Autores: Ito,Vivian Cristina; Zielinski,Acácio Antônio Ferreira; Demiate,Ivo Mottin; Spoto,Marta; Nogueira,Alessandro; Lacerda,Luiz Gustavo.
Abstract The effects of gamma radiation (0, 1, 2 and 3 kGy) were used to evaluate the stability and thermal degradation kinetics of anthocyanins, as well as the stability of total phenolic compounds (TPC) and antioxidant activity at different temperatures (4, 25, 35 and 45 °C) during the storage (0, 30, 60, 90 and 120 days) of black rice flour. This flour can be used as ingredient for gluten-free cereal products with higher nutritional value. For this it is necessary to preserve the anthocyanin content during thermal processing and shelf-life periods. At time 0, the dose of 3 kGy provided all of the most available bioactive compounds, raising their antioxidant potential, except for TPC. During the storage at different temperatures up to 120 days, gradual...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthocyanins; Bioactive compounds; Irradiation; Stability; Thermodynamic parameters; Pigmented rice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100500

Antimicrobial effectiveness of maleimides on fungal strains isolated from onychomycosis
Provedor de dados: 52
Autores: Gayoso,Carla Wanderley; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Cechinel Filho,Valdir; Trajano,Vinícius Nogueira; Pereira,Fillipe de Oliveira; Lima,Igara Oliveira.
This study aimed to analyze the effectiveness of maleimides as inhibitors on the growth of fungal strains isolated from onychomycosis by the solid medium diffusion procedure. The results showed a promising antifungical activity of the assayed maleimides with formation of fungal growth inhibition halos oscillating between diameter 10 and 23mm. MIC was 100µg/mL for 3,4-dichloro-N-phenyl-methyl-maleimide and 3,4-dichloro-N-phenyl-propil-maleimide and 200µg/mL for 3,4-dichloro-N-phenyl-maleimide, 3,4-dichloro-N-phenyl-ethyl-maleimide and 3,4-dichloro-N-phenyl-buthyl-maleimide.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Onychomycosis; Fungi; Maleimides; Antifungical activity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500016

Use of Adsorbent Biochar from Pequi (Caryocar Brasiliense) Husks for the Removal of Commercial Formulation of Glyphosate from Aqueous Media
Provedor de dados: 52
Autores: Borba,Lana Lima; Cuba,Renata Medici Frayne; Terán,Francisco Javier Cuba; Castro,Martha Nascimento; Mendes,Thiago Augusto.
Abstract This study evaluated the capacity of adsorbent biochar derived from pequi husks to remove glyphosate (commercial formulation) in aqueous medium under three pH conditions (5.5, 7.0 and 8.0). This biochar presented a mean yield of 33.1% ± 2.66% and a high amount of surface particles of small dimensions endowing it with high surface area. The results showed that removal is proportional to pH increase in the range of 5.5 to 8.0. Adsorption assays performed at pH 7 and 8 fitted better to the Langmuir pseudo-first order kinetics model with fast adsorption in the first 15 to 30 minutes. The results for the acidic pH range fit none of the adopted models satisfactorily. The results obtained suggest that adsorbent can be used as an efficient and inexpensive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agroindustrial waste; Glyphosate commercial formulation; Biochar; Adsorption.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100603

Seasonal and spatial variability of appendicularian density and taxonomic composition in the Caravelas Estuary (Northeastern Brazil) and adjacent coastal area
Provedor de dados: 52
Autores: Carvalho,Pedro Freitas de; Bonecker,Sérgio Luiz Costa.
This study aimed to identify and assess the seasonal and spatial variations of the appendicularians in the Caravelas River estuary and the adjacent coastal area. Samples were taken during 12 campaigns over five years (2001 and 2003-2006). Ten species were identified; the most abundant were Oikopleura dioica, Oikopleura rufescens, and Oikopleura longicauda. These species represented more than 95% of the total numbers of appendicularians. The remaining species were less frequent and occurred in low densities. The mean density of appendicularians found at the coastal stations (804 ind.m-3.) was higher than in the estuary (66 ind.m-3). However, the differences observed between the estuary and coastal stations were not significant (p=0.54). The samples taken...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oikopleuridae; Fritillariidae; Tropical estuary; Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100020

Effect of water losses by evaporation and chemical reaction in an industrial slaker reactor
Provedor de dados: 52
Autores: Andreola,Ricardo; Vieira,Osvaldo; Santos,Onélia Aparecida Andreo dos; Jorge,Luiz Mario de Matos.
A dynamic model of the slaker reactor was developed and validated for Klabin Paraná Papéis causticizing system, responsable for white liquor generation used by the plant. The model considered water losses by evaporation and chemical reaction. The model showed a good agreement with the industrial plant measures of active alkali, total titratable alkali and temperature, without the need of adjustment of any parameter. The simulated results showed that the water consumption by the slaking reaction and evaporation exerted significant influence on the volumetric flow rate of limed liquor, which imposed a decrease of 4.6% in the amount of water in reactor outlet.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Slaker reactor; Modeling and simulation; White liquor.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200019

Effects of Leached Amylose and Amylopectin in Rice Cooking Liquidon Texture and Structure of Cooked Rice
Provedor de dados: 52
Autores: Yang,Liu; Sun,Yong-Hai; Liu,Yang; Mao,Qian; You,Li-Xin; Hou,Ju-Min; Ashraf,Muhammad Aqeel.
ABSTRACT Effects of leached amylose (AM) and amylopectin (AP) on textural and morphological properties of cooked rice were investigated separately by replacing cooking liquid with AM and AP separated from pouring cooking liquid. The pouring of cooking water reduced the hardness (from 28.45 to 19.42N) and stickiness (from 1.74 to 1.19N·s) significantly. However, the addition of AM and AP enhanced the hardness (27.63N) and stickiness (1.71N·s).Scanning electron microscopy show that the leached short-chain AM entered the surface hollows in the cooked rice after water evaporation. Meanwhile, the Long-chain AM cross-linked to formed a three-dimensional network structures, which covered on the filled hollows. This distribution led to a harder texture of cooked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylose; Amylopectin; Leached; Morphological properties; Textural properties.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200500

Effect of the improved fermentation on physicochemical properties and sensorial acceptability of sour cassava starch
Provedor de dados: 52
Autores: Marcon,Maria Janete Angeloni; Vieira,Gisele Cristina Netto; Simas,Karina Nunes de; Santos,Karina; Vieira,Manoela Alano; Amboni,Renata Dias de Mello Castanho; Amante,Edna Regina.
The aim of this work was to study the effect of improved fermentation on sour cassava starch, aiming to reduce its fermentation time and to enhance its expansion capacity as well as its viscoamylographic properties and its sensorial acceptability. Results showed that the improved process of cassava starch production did not harm starch expansion, physicochemical properties or sensorial acceptability; it also produced starches with different viscoamylographic properties, which compared favourably to those of the sour cassava starch produced through current industrial methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sour cassava starch; Expansion; Viscosity; Sensorial acceptability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700018

Erratum: Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300906

Copper concentration of vineyard soils as a function of pH variation and addition of poultry litter
Provedor de dados: 52
Autores: Nachtigall,Gilmar Ribeiro; Nogueirol,Roberta Corrêa; Alleoni,Luís Reynaldo Ferracciú; Cambri,Michel Alexandro.
Copper (Cu) concentration was evaluated as a function of pH variation and addition of poultry litter to a Dystrophic Lithic Udorthent and a Humic Dystrudept from the state of Rio Grande do Sul, Brazil, cultivated with vines treated with successive applications of Cu-based product. Samples were collected from the surface layer (0 to 10 cm). Soluble Cu concentration was determined using DTPA and Mehlich III as extractants, and exchangeable Cu was determined in CaCl2. The availability of Cu was mainly affected by the soil pH. CaCl2 extractant had the best correlation with Cu concentration in contaminated soils, according to treatments applied. The addition of poultry litter did not reduce Cu availability in these soils. Total soil Cu content varied between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vineyard soils; Chemical extractors; PH and soil organic matter.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700005

Cacao yield in different planting densities
Provedor de dados: 52
Autores: Souza,Carlos Alberto Spaggiari; Dias,Luiz Antônio dos Santos; Aguilar,Marco Antonio Galeas; Sonegheti,Sabrina; Oliveira,Jéferson; Costa,Jorge Luiz Andrade.
The effect of six planting densities on cacao yield of a commercial hybrid mixture as well as the interaction of planting densities with the years were investigated. Crop data collected over a 14-year period (1977-1990) showed that it was possible to optimise the regional cacao yields by implementing high planting densities (2500 and 1736 trees ha-1). This was however only true for the first half of the crop period. In the second half, low planting density (1059 trees ha-1) attained the best yields. This change in the ranking of planting densities over the years confirmed the presence of density-year interaction. Alternatives to achieve high productivity in high planting density systems were presented and discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Density-year interaction; High productivity; Theobroma cacao L.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600001

Phenetic analysis of Panstrongylus megistus Burmeister, 1835 (Hemiptera: Reduviidae: Triatominae) in the State of Paraná-Brazil
Provedor de dados: 52
Autores: Kopp,Rogério Luiz; Thomaz-Soccol,Vanete; Klisiowicz,Débora do Rocio; Membrive,Norberto; Barata,José Maria Soares; Jurberg,José; Steindel,Mário; Kopp,Denize Cristina Trevisan; Castro,Edilene Alcântara de; Luz,Ennio.
Panstrongylus megistus is an important Chagas Disease vector and is said to be one of the species that might replace Triatoma infestans as the main vector of that disease in Brazil. The different degrees of P. megistus domiciliation in Brazil and its epidemiological relevance draw forth the need for the development of genetic studies that make it possible to analyze and understand the interchange of individual and gene fluxes among different populations. Thus, the present work aimed at studying the genetic variability of P. megistus in the State of Paraná - south of Brazil- and at comparing it with populations of the same species from five other states in Brazil (SP, MG, SC, RS, SE). In order to attain the proposed objective, 25 populations were studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panstrongylus megistus; Isoenzymes; Genetic variability; Triatomines.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200012

Size-associated Variation and Factors Affecting the Morphology of Brown Bodies in Glycera tridactyla (Polychaeta:Glyceridae)
Provedor de dados: 52
Autores: Can,Elif.
Size-associated variations in brown body morphology (size and shape) were studied from the individuals of Glycera tridactyla. Variables related to size, length (BL) and width (BW) of the brown bodies were measured. On the basis of these measurements, other variables such as the surface area (SA), volume (V), surface area/volume ratio (SA/V) and elongation degree (ED) were calculated. Brown body shape was quantified by the elongation degree. The immature brown bodies were significantly smaller than the mature bodies, and therefore the smaller bodies had a higher SA/V. Results obtained from the regression analysis showed that there was a significant relationship between all the variables with the exception of SA/V:BW for the immature bodies and ED: BL for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bloodworm; Elongation degree; Encapsulation; Proboscis; Surface area volume ratio.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600941

Influence of Ionizing Radiation on Biodegradable Foam Trays for Food Packaging Obtained from Irradiated Cassava Starch
Provedor de dados: 52
Autores: Brant,Antônio Jedson Caldeira; Naime,Natália; Lugão,Ademar Benévolo; Ponce,Patrícia.
ABSTRACT Biodegradable polymers, such as starch, cellulose, and derivatives thereof, are being studied to produce innovative packaging in the most diverse shapes (films, bags, trays, bottles, etc.) to attend this current market trend. The aim of this work was to produce foam trays from cassava starch for food packaging via extrusion followed by thermoforming process. Their formulations were based on unirradiated and γ-irradiated starches at diverse radiation absorbed doses (in kGy) in order to evaluate the influence of the irradiated starches on the physical properties of the trays. Water absorption results showed an irregular increase with the increase of the absorbed dose ( for example, 26.32% and 39.84% for the trays based on unirradiated starch 0 kGy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradable foam; Cassava starch; Food packaging; Ionizing radiation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100604

Energy Management in Energy-Intensive Industries: Developing a Conceptual Map
Provedor de dados: 52
Autores: Silva,Vilson Roiz Gonçalves Rabelo da; Loures,Eduardo de Freitas Rocha; Lima,Edson Pinheiro de; Costa,Sergio Eduardo Gouvêa da.
Abstract This paper presents the development of a conceptual map regarding energy management applied to industry. The energy issue is currently of great relevance, especially for the so-called energy-intensive industries related to high energy consumption and their associated environmental impacts. The present research is characterized as a basic, exploratory approach justified by the need to build knowledge on the subject of energy management in industry. The methodology provides for the use of a computational tool called CMap Tools, which assists in the graphic representation of the proposed conceptual map. The conceptual map based on the ISO 50001 standard and on successful energy management practices described in the scientific literature is directed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy management system; Energy-intensive industries; Conceptual map.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200216

Radionuclide imaging of musculoskeletal infection
Provedor de dados: 52
Autores: Palestro,Christopher J.; Love,Charito.
Radionuclide imaging studies are routinely used to evaluate patients suspected of having musculoskeletal infection. Three-phase bone imaging is readily available, relatively inexpensive, and very accurate in the setting of otherwise normal bone. Labeled leukocyte imaging should be used in cases of "complicating osteomyelitis" such as prosthetic joint infection. This test also is useful in clinically unsuspected diabetic pedal osteomyelitis as well as in the neuropathic joint. It is often necessary, however, to perform complementary bone marrow imaging, to maximize the accuracy of labeled leukocyte imaging. In contrast to other regions in the skeleton, labeled leukocyte imaging is not useful for diagnosing spinal osteomyelitis. At the moment, gallium is the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musculoskeletal infection; Osteomyelitis; Bone scintigraphy; Labeled leukocytes; Gallium; FDG-PET.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600003

Nano Coated Lead Free Solders for Sustainable Electronic Waste Management
Provedor de dados: 52
Autores: Geethan,K. Arun Vasantha; Jose,S.; Kannan,S. Ashwin; Durai Raj,J. Immanuel; k.,Ramesh.
ABSTRACT Lead has been used in a wide range of applications, but in the past decades it became clear that its high toxicity could cause various problems. Studies indicate that exposure to high concentrations of lead can cause harmful damages to humans. To eliminate the usage of lead in electronic products as an initiative towards electronic waste management (e waste), lead free solders were produced with suitable methods by replacing lead. But lead free solders are not preferred as a substitute of lead because they are poor in their mechanical properties such as tensile strength, shear strength and hardness which are ultimately required for a material to resist failure.Nano-Structured materials and coatings offer the potential for Vital improvements in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E-waste; Nano-structured materials; Lead free; Management.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300607

Efficiency of RAPD and ISSR markers in assessing genetic variation in Arthrocnemum macrostachyum (Chenopodiaceae)
Provedor de dados: 52
Autores: Saleh,Basel.
The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002

Sobre a Padronização de um Método Prático Para a Dosagem da Cafeína
Provedor de dados: 52
Autores: Bührer,Nilton E.
In this work the author puts in evidence a practical and at the same time rapid method for the determination of the cafein quantity in erva-mate, that may be utilized to perfection in food and industrial analyses or others that not demand scientific rigour. The author presents a description of the method and makes a comparison with the classical method of Grandval-Lajoux, showing that the differences between the one and the other are practically nil. In conclusion, the result of the three analyses of the same sample of erva-mate, made by the Grandval-Lajoux method are cited, show that the same differences appear as those shown between the rapid method suggested by the author and the classical method. In this case, the maximum difference was 0,06% and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeine; Erva-mate; Caffeine assay.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500007

Phenotypic Characterization and Genetic Diversity of Sugarcane Varieties Cultivated in Northern Karnataka of India based on Principal Component and Cluster Analyses
Provedor de dados: 52
Autores: Tawadare,Rakesh; Thangadurai,Devarajan; Khandagave,Rayappa Bharamappa; Mundaragi,Abhishek; Sangeetha,Jeyabalan.
Abstract: Sugarcane is a major commercial crop grown in India and across the world. Hence, several elite varieties have been developed now-a-days to overcome many obstacles including abiotic stresses and diseases. The present study was undertaken to screen genetic variation among twenty four sugarcane varieties that are commonly cultivated across Northern Karnataka, India with reference to physicochemical characters. Experiment was conducted in triplicate following randomized complete block design (RCBD) at S. Nijalingappa Sugar Institute, Belagavi, Karnataka, India during February 2016-17. Physiological parameters such as internode length, stalk height, plant height, stalk girth, number of internodes, single cane weight, single cane volume of juice, cane...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physicochemical characters; Principal component analysis; Agglomerative hierarchical clustering; Sugarcane.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100215

Lake Baikal Endemic Sculpins (Cottoidei): A Promising Model to Study Adaptive Plasticity of Blood Cholesterol Metabolism
Provedor de dados: 52
Autores: Sudakov,Nikolay P.; Klimenkov,Igor V.; Pastukhov,Mikhail V.
We analyzed the blood lipid spectra in four closely related sculpin (Cottoidei) species endemic to Lake Baikal. These data characterize the Baikal sculpins as a set of model organisms for studying the adaptive plasticity of cholesterol metabolism and also mechanisms of resistance to the development of dyslipidemia and atherosclerosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol; Lipoproteins; Liver; Cottoidei; Lake Baikal.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400613

Characterization of the Physicochemical Properties of Different Biodiesel Samples
Provedor de dados: 52
Autores: Vertonha,Maria Thais; Delgado,Leonardo Almeida; Lukasievicz,Gustavo Vinicius Bassi.
ABSTRACT For combustion engines to perform well, it is necessary that the fuel used be of high quality. Fuel quality can be analyzed through different physicochemical properties. This study presents comparisons between the values presented in literature, legislation and those obtained through experimental tests of different biodiesel samples. Biodiesel is a promising alternative of renewable energy obtained from raw material. The properties observed in this study are: Viscosity, density, cloud point, pour point and calorific value. The data explored by this work aims to bring solidification to the methodologies and equipment necessary for a better characterization of biodiesel.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiesel; Vegetable oil; Animal fat; ANP.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200236

LED Lights Enhance Metabolites and Antioxidants in Chinese Cabbage and Kale
Provedor de dados: 52
Autores: Lee,Min Ki; Arasu,Mariadhas Valan; Park,Suhyoung; Byeon,Dong Hae; Chung,Sun-Ok; Park,Sang Un; Yong-,PyoLim; Sun-,JuKim.
Light emitting diode (LED) lights play an important role in the plant physiology and alter the metabolites in a significant manner. Glucosinolates (GSLs), polyphenols, flavonoids and antioxidant properties of Chinese cabbage and kale cultivated in varying LED lights were investigated. Analysis revealed 7 aliphatic, 3 indolyl and 1 aromatic GSLs in Chinese cabbage and kale. The total GSL content ranged from 1.5-19.08 and 1.85-24.87 µmol/g DW, and glucobrassicanapin was the predominant GSL (3) in Chinese cabbage, whereas; sinigrin (3.49 µmol/g DW) was in kale. Blue and red LED lights produced significantly higher amount of GSLs in Chinese cabbage and kale respectively. Results revealed higher amount of total polyphenol (3.845 µg/mL) and total flavanoids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chinese cabbage; Kale; Glucosinolates; Polyphenols; Flavanoids; Antioxidant activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100601

Antibacterial Activity of Polygonum Orientale Extracts Against Clavibacter Michiganensis subsp. michiganensis, the Agent of Bacterial Canker of Tomato Disease
Provedor de dados: 52
Autores: Cai,Jin; Gao,Yichen; Wang,Mengliang; Zhang,Jing; Zhang,Yongbin; Wang,Qi; Liu,Jiquan.
Abstract Clavibacter michiganensis subsp. michiganensis (Cmm), which is a Gram positive bacterium, causes the bacterial canker of tomato disease. The purpose of the study was to evaluate the antibacterial activity of Polygonum orientale extracts against Cmm. In this study, firstly, effects of three extracting parameters (extractive time, extractive temperature, and solid to liquid ratio) of orthogonal experiment design L27 (313) were conducted. Secondly, survival rate was determined and inhibition zone of Cmm rescued post-stress was monitored. Finally, extracellular OD260nm value, extracellular protein content, conformational structure of membrane protein, extracellular alkaline phosphatase (AKP) activity, and ATPase activity were measured to investigate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant bioactive compounds; Antibacterial agent; Plant pathogenic bacterium; Orthogonal experiment design; Biological control.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100217

The use of fricke dosimetry for low energy x-rays
Provedor de dados: 52
Autores: Austerlitz,Carlos de; Souza,Viviane; Villar,Heldio Pereira; Cordilha,Aloisio.
The performance of four X-ray qualities generated in a Pantak X-ray machine operating at 30-100 kV was determined with a parallel-plate ionization chamber and a Fricke dosimeter. X-ray qualities used were those recommended by Deutsch Internationale Normung DIN 6809 and dose measurements were carried out with Plexiglas® simulators. Results have shown that the Fricke dosimeter can be used not only for soft X-ray dosimetry, but also for the maintenance of low-energy measuring systems' calibration factor.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiation dosimetry; Soft X-rays; Fricke dosimeter.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200004

Does adaptive strategy for delayed seed dispersion affect extinction probability of a desert species? an assessment using the population viability analysis and glass house experiment
Provedor de dados: 52
Autores: Mathur,Manish.
Canopy seed bank is an important adaptive evolutionary trait that provides various types of protection to the seeds. However, costing of such evolutionary trait on plant survival is largely unknown. Present investigation provided a new insight on the serotonious habit of Blepharis sindica associated with its endangerment status. Extinction probabilities of two available population of B. sindica were quantified using two types of census data, i.e., fruiting body number and actual population size. Population Viability Analysis (PVA) revealed that delayed seed release tendency (higher fruiting body number) was not synchronized with actual ground conditions (lower population size). PVA analysis based on actual population size indicated that both the available...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blepharis sindica; Population Viability Analysis (PVA); Serotiny; Cumulative Probability of Extinction; Mean Extinction Time; Hydrate Mucilage.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500774

Fishing activity in Northern Rio de Janeiro State (Brazil) and its relation with small cetaceans
Provedor de dados: 52
Autores: Beneditto,Ana Paula Madeira Di; Ramos,Renata Maria Arruda; Lima,Neuza Rejane Wille.
Research on fishing activity at Atafona village, in Northern Rio de Janeiro, Brazil (21°35'S), was carried out between 1987-96 for the purpose of relating it to the accidental capture of small cetaceans and of estimating the relationship between fishing activity and the diet of small cetaceans. Data on fishing operations were obtained at the cold storage plants management, from interviews with fishermen and personal observations. The most representative fishing resources were Xyphopenaeus kroyeri, Micropogonias furnieri, Carcharhinus plumbeus, C. acronotus,and Rhizoprionodon porosus. Gillnets are responsible for the accidental capture of small cetaceans in the region, mainly Pontoporia blainvillei and Sotalia fluviatilis (marine form). Four types of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fishery; Accidental capture; Pontoporia blainvillei; Sotalia fluviatilis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300004

Triplaris americana L. (Polygonaceae), a New Host Plant For Aethalion reticulatum (Linnaeus, 1767) (Hemiptera: Aethalionidae)
Provedor de dados: 52
Autores: Pires,Evaldo Martins; Silva,Larissa Cavalheiro; Batirola,Leandro Denis; Nogueira,Roberta Martins; Barreto,Marliton Rocha; Corassa,Janaina Nadai.
Triplaris americana is a plant that has been applied as ornamental specie and also as natural medicine. Adults and immature stages of Aethalion reticulatum were observed colonizing specimens of this plant in Sinop, MT, Brazil, which represent the first record of this leafhopper colonizing this specie.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ants; Bees; Geographic distribution; Honeydew.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100031

Aerial line transect survey to estimate abundance of marsh deer (Blastocerus dichotomus) (Illiger, 1815)
Provedor de dados: 52
Autores: Andriolo,Artur; Piovezan,Ubiratan; Costa,Mateus José Rodrigues Paranhos da; Laake,Jeff; Duarte,José Maurício Barbanti.
The objective was to estimate abundance of marsh deer in the Paraná River basin of this work. The results provided information to support further analysis of the impact of the Porto Primavera flooding lake over population. Sixty-nine animals were recorded by aerial survey using distance sampling methodology. Animals were widely distributed throughout the study area. The uncorrected data resulted in a estimate density of 0.0035 ind/ha and a population size of 636 individuals. Correcting the g for the animals that could be missed the calculated abundance was 896 (CV=0.27) individuals. This methodology was applied with success to survey marsh deer. The result was important to evaluate the marsh deer status in the area, and for future analysis of the impact of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abundance; Aerial Survey; Blastocerus dichotomus; Marsh deer; Distance Sampling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600017

Activities, procedures and doses in pediatric patients due to radiopharmaceuticals
Provedor de dados: 52
Autores: Oliveira,Silvia Maria Velasques de; Boasquevisque,Edson Mendes; Santos,Felipe Sima dos; Biancardi,Rodrigo.
An investigation performed between 2003 and 2005 in sixteen selected public and private institutions in Northeast, Southeast and South geographical regions of Brazil evaluated average organ doses and effective doses in 2,411 pediatric patients due to diagnostic procedures with radiopharmaceuticals. For 1 year, effective doses were greater than literature. For 5 years, differences were noticed between present work and literature for bone scintigraphy, thyroid scintigraphy and 67Ga citrate scintigraphy. These differences may be attributed to the uncertainties in internal dose calculations. High absorved doses in bone surfaces of children due to 67Ga citrate and bone scintigraphy should be evaluated accordingly. Current protocols used recommend standardized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediatrics; Oncology; Dosimetry; Radiopharmaceuticals; Nuclear medicine; Radiation protection.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700019

Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct
Provedor de dados: 52
Autores: Martin,Natalia; Souza,Simone Regina de; Silva,Roberto da; Gomes,Eleni.
Pectin lyase and polygalacturonase production by newly isolated fungal strains was carried out in solid-state fermentation. Moniliella SB9 and Penicillium sp EGC5 produced polygalcturonase (PG) and pectin lyase (PL) on mixture of orange bagasse, sugar cane bagasse and wheat bran as substrate. PG and PL produced by Moniliella presented optimum activity at pH 4.5 and 10.0 and at 55 and 45°C, respectively, while these enzymes from Penicillium sp presented optimum activity at pH 4.5-5.0 and 9.0 and 40°C, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium; Moniliella; Polygalacturonase; Pectin lyase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500018

Chemical composition of fruits of some important chestnut cultivars
Provedor de dados: 52
Autores: Ertürk,Ümran; Mert,Cevriye; Soylu,Arif.
In this study, chemical compositions of the fruits of some important domestic chestnut types and cultivars were investigated. They contained (g/100g dry matter basis) total carbohydrates 75.32 - 86.31, total sugar 10.32 - 22.79, invert sugar 0.08 - 1.25, starch 54.45 - 69.70, sucrose 8.86 - 21.28, ash 1.02 - 3.22, crude cellulose 3.58 - 5.96, total fat 0.49 - 2.01, total protein 4.88 - 10.87. Ca, Mg, Fe, Mn, Cu, Zn, P, Na and K contents were (mg/100g) 43 - 230, 70 - 160, 0.4 - 5.7, 0.7 - 5.5, 0.6 - 3.8, 1.8 - 9.1, 107 - 191, 6 - 41, 761 - 1271, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Castanea sativa; Chestnut; Fruit Composition.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300001

Resistance profile to antimicrobials of Salmonella spp. isolated from human infections
Provedor de dados: 52
Autores: Delicato,Elaine Regina; Mikcha,Jane Martha Graton; Fernandes,Sueli Aparecida; Pelayo,Jacinta Sanchez.
The purpose of this study was to analyse the profile of antimicrobial resistance among 21 strains of Salmonella isolated from patients with gastroenteritis symptom. It was observed that S. enteritidis was the serotype prevalent. These strains were sensitive to the majority of the antimicrobials tested, however, high resistance was observed in S. typhimurium and S. enterica subsp. enterica serotype 4,5,12:i:-. Surveillance and an efficient monitoring should be priority for the public health for the containment of antimicrobial resistance in foodborne infections.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Humans; Serotypes; Resistance; Antimicrobials.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200005

Effect of Gamma Radiation on Growth and Metabolic Activities of Arthrospira platensis
Provedor de dados: 52
Autores: Abomohra,Abo El-Fatah; El-Shouny,Wagih; Sharaf,Mona; Abo-Eleneen,Mai.
This work aimed to study the influence of gamma radiation on the growth and production of some active substances of Arthrospira platensis. Biomass production was significantly inhibited (p ≤ 0.05) by 21 and 34%, with respect to the control at 2.0 and 2.5 kGy, respectively. Chlorophyll-a content showed 11% reduction at 2.5 kGy compared to the control. As a result of growth and Chl-a inhibition, chlorophyll productivity recorded a continuous significant decrease below the control in the cells exposed to 1, 1.5, 2 and 2.5 kGy by 8, 12, 15 and 25%, respectively after 15 days of incubation. In addition, phycobillins productivity showed significant decrease by 10 and 36% below the control at 2 and 2.5 kGy of gamma radiation, respectively. Protein production...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthrospira platensis; Spirulina platensis; Gamma radiation; Metabolic activity; Nutritional value.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100409

Inulin Induces IL-10 Secretion and Increased FOXP3 Gene Expression in Human Peripheral Blood Mononuclear Cells
Provedor de dados: 52
Autores: Amarante,Marla Karine; Ariza,Carolina Batista; Oliveira,Carlos Eduardo Coral de; Gualtieri,Karina de Almeida; Oda,Julie Massayo Maeda; Watanabe,Maria Angelica Ehara.
ABSTRACT Inulin is an effective prebiotic and its potential in modulating systemic immunity have been proposed. A subpopulation of T cells, named T regulatory cells (Tregs), expressing the Forkhead boxP3 transcription factor are key mediators of peripheral tolerance and suppress undesirable immune responses. These Tregs can be induced by cytokine transforming growth factor beta (TGF-β) and interleukin 10 (IL-10). This work aimed to evaluate inulin effects on human peripheral blood mononuclear cells (PBMC) in vitro. PBMC were incubated with inulin, and the expression of TGF-(1, FOXP3 and IL-10 was analyzed. Increased supernatant IL-10 levels were observed in PBMC of inulin-treated group (p=0.03). Moreover, FOXP3 gene expression was 7.6 fold higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulin; FOXP3; TGF-β1; IL-10; PBMC.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100417

Pre-eclampsia: a life-threatening pregnancy syndrom
Provedor de dados: 52
Autores: Brazdova,Andrea; Keprova,Alena; Zidkova,Jarmila; Madar,Jindrich.
Pre-eclampsia is a serious pathological state affecting 5-10% of pregnant women. Currently, it is diagnosed in the second half of pregnancy, particularly after the 20th week. Symptoms mostly correspond to the changes of blood vessels and kidneys. The severity of pre-eclampsia is proportional to symptomatic manifestations, thus the more symptoms present, the higher is of pre-eclampsia development. Although there are several studies dealing with pre-eclampsia pathology, the complete etiology is still unknown. In this review paper, several theories are presented and discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Placenta; Pre-eclampsia; Pregnancy; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500701

Ethanol fermentation of a diluted molasses medium by Saccharomyces cerevisiae immobilized on chrysotile
Provedor de dados: 52
Autores: Monte Alegre,Ranulfo; Rigo,Maurício; Joekes,Inés.
In this work, the catalytic role of chrysotile support on the acceleration of alcoholic fermentation under non-aseptic conditions by Saccharomyces cerevisiae was investigated. The fermentation medium employed consisted only of diluted sugar-cane molasses. In the batch fermentations process with immobilized yeasts, the initial rate of CO2 production increased roughly 27 % during the first 30 minutes, compared to systems containing no chrysotile. A study of continuous alcoholic fermentation with chrysotile in the reactor bed showed a higher ethanol production rate at the different dilution rates investigated compared to similar fermentations without chrysotile.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholic continuous fermentations; Alcoholic batch fermentations; Immobilized yeast; Chrysotile; Ethanol.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400031

Harvesting Microalgal Biomass grown in Anaerobic Sewage Treatment Effluent by the Coagulation-Flocculation Method: Effect of pH
Provedor de dados: 52
Autores: Cassini,Servio Tulio; Francisco,Sara Aparecida; Antunes,Paulo Wagner Pereira; Oss,Rodrigo Nunes; Keller,Regina.
ABSTRACT Harvesting is a critical step in microalgal biomass production process for many reasons. Among the existing techniques available for harvesting and dewatering microalgal biomass, recovery from aqueous medium by coagulation-flocculation has been the most economically viable process, althoughit is highly dependent on pH. This study aims to assess alternative coagulants compared to the standard coagulant aluminum sulfate for microalgal biomass recovery from anaerobic effluent of domestic sewage treatment. The effluent quality was also analyzed after biomass recovery. Coagulants represented by modified tannin, cationic starch and aluminum sulfate recovered more than 90% of algae biomass, at concentrations greater than 80 mg/L, in the pH range 7-10....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalgae; Anaerobic effluent; Coagulation-flocculation; PH.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100700

Hepatoprotective Role of α-Lipoic acid and Thymoquinone in Acetaminophen- Induced Liver Injury: Down-Regulation of COX-2 and flt-1 Expression
Provedor de dados: 52
Autores: Al-Rasheed,Nawal M.; Fadda,Laila; Al-Rasheed,Nouf M.; Hasan,Iman H.; Ali,Hanaa M.; Al-Fayez,Musaed; Mohamad,Raeesa A.
ABSTRACT Acetaminophen (APAP) is a widely-used analgesic, while toxic doses of which induce liver injury. Inducible cyclooxygenase-2 (COX-2) is derived prostaglandins which play an anti-inflammatory role in acetaminophen-induced hepatotoxicity. Selective activation of vascular endothelial growth factor (VEGFR1, flt -1) on endothelial cells increased mRNA levels of hepatocyte mitogens (IL-6) and hepatocyte growth factor leading to prosurvival effects on hepatocytes. The aim of this study was to compare the hepatoprotective effect of N-acetylcysteine (NAC; the antidote for APAP) with that of α-Lipoic acid (ALA) and/or Thymoquinone (THQ) either alone or in combination on liver injury induced by APAP. APAP administration elevated most of the previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetaminophen; Α-Lipoic acid; Thymoquinone; Cyclooxygenase-2.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100427

Biologically Synthesized Silver Nanoparticles for Enhancing Tetracycline Activity Against Staphylococcus aureus and Klebsiella pneumoniae
Provedor de dados: 52
Autores: Hussein,Elsayed Alsaied Masoud; Mohammad,Ali Al-Hajry; Harraz,Farid Abourageh; Ahsan,Mohd Faisal.
Abstract Phytochemical content of plant extracts can be used effectively to reduce the metal ions to nanoparticles in one-step green synthesis process. In this study, six plant extracts were used for the synthesis of silver nanoparticles (AgNPs). Biologically synthesized AgNPs was characterized using UV-Vis Spectrophotometer, Field Emission Scanning Electron Microscope (FE-SEM), X-ray diffraction (XRD), Energy Dispersive X-ray spectroscopy (EDX) and Fourier Transform Infrared (FTIR) spectroscopy. The individual and combined effects of AgNPs and tetracycline against S. aureus and K. pneumoniae were assessed. Ginger, onion and sidr extracts supported AgNPs formation while arak, garlic and mint extracts failed to convert the silver ions to AgNPs. The present...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver nanoparticles; Biosynthesis; Plant extracts; Tetracycline; S. aureus; K. pneumoniae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100308

Effect of NaCl and HCl concentrations on primary pH measurement for the certification of standard materials
Provedor de dados: 52
Autores: Souza,V.; Ordine,Alberto Pires; Fraga,I. C. S.; Getrouw,Monique Audrey; Borges,P. P.; Damasceno,J. C.; Couto,P. R. G.
pH is an important parameter to control the production of food, medicine, petrochemical products and others. Thus, standard reference materials used to calibrate pH meters are necessary to guarantee reliability of the measurements. The Chemical Metrology Division (Dquim) of the National Metrology Institute of Brazil (Inmetro) has as one of its missions the certification of standard reference materials. In the case of pH, it will be done by the pH primary measurement system of Inmetro, in operation at Dquim since 2003. The buffer solution of nominal pH value equal to 6.865 will be the first to be certified. The study of some measurement parameters has already been accomplished, and will lead to the improvement of the primary pH measurement. In this paper,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical metrology; Primary pH; Reference material.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200013

Influence of vitamin E supplemented diet on charque quality and lipid stabilization
Provedor de dados: 52
Autores: Facco,Elizete Maria Pesamosca; Lage,Moacir Evandro; Godoy,Helena Teixeira.
The present work evaluated and compared the lipid oxidation of charque, taken from, the semi-tendinosus muscle of animals from the Nelore breed (Bos taurus indicus), supplemented with α-tocopherol. Charque was produced submitting the samples to salt moisture, salt drying and exposition to the sun for dehydration until water activity values (Aw) reached 0.75. The statistical analysis of the results showed that there were no significant differences in the values of cholesterol, total lipids, fatty acids and substances relative to tiobarbituric acid (TBARS) and tocopherol as the samples were controlled and supplemented, respectively. Despite this, the values of TBARS and cholesterol were lower in the samples with the supplement of α-tocopherol. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Charque; Lipid oxidation; Tocopherol; Vitamin; Supplementation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300025

Estimation of aerial biomass of Lychnophora ericoides (Mart.)
Provedor de dados: 52
Autores: Andrade,Brunno Santana de; Hay,John Du Vall.
For sustainable use of native plant species, knowledge of the amount of harvestable biomass is necessary. This study presents data on allometric relationships of Lychnophora ericoides Mart. (Asteraceae), an extractive resource in the Cerrado region of Brazil. On the Fazenda Água Limpa (15º 45'S, 47º 57'W) of the Universidade de Brasilia, 38 individuals of this species were measured in the field, the parts above ground were harvested, separated into components and oven dried. The best regression equations to estimate biomass were geometric and the best fit was between total height and total biomass (r² = 0.923). The economically useful portions, the leaves and branches accounted for approximately 20% of total above ground dry weight, but when used as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allometric relationships; Sustainable development; Cerrado.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400014

Prevalence of metabolic syndrome among Kaingang native americans in southern Brazil
Provedor de dados: 52
Autores: Anjos,Heloisa Nakai Kwabara dos; Toledo,Max Jean de Ornelas; Mota,Lúcio Tadeu; Previdelli,Isolde Terezinha Santos; Anjos,Adriano Félix dos; Saruhashi,Tiago Ribeiro; Carrara,Márcia Aparecida; Batista,Márcia Regina.
The aim of this work was to evaluate the prevalence of metabolic syndrome, diabetes mellitus, and obesity among a Brazilian indigenous population. A cross-sectional study was carried out in 2008 among Kaingang native Americans from the central region of the state of Paraná, Brazil. Eighty two of the inhabitants aged 15 or older were selected. Height, weight, blood pressure, waistline circumference, and hip circumference were measured. After fasting, the blood was collected for the measurement of glucose, HDL cholesterol, triglyceride, total cholesterol, AI and B apolipoprotein, and hemoglobin. The prevalences found were: fasting hyperglycemia (9.8%), hypercholesterolemia (4.9%), reduced HDL cholesterol (13.4%), hypertriglyceridemia (11%), abdominal obesity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Health of indigenous peoples; Brazilian native Americans; Kaingang.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100011

A Rapid and Reliable Method for Molecular Detection of Fusarium guttiforme, the Etiological Agent of Pineapple Fusariosis
Provedor de dados: 52
Autores: Carnielli-Queiroz,Lorena; Fernandes,Patricia Machado Bueno; Fernandes,Antônio Alberto Ribeiro; Ventura,José Aires.
Abstract Pineapple (Ananas comosus var. comosus) fusariosis is an economically important fungal disease affecting the plant and its fruit. A rapid and reliable diagnosis is the base of integrated disease management practices. Fusariosis has resulted in quarantines for pineapple products in Central America, Africa and Asia. Difficulties diagnosing and correctly identifying the fungus Fusarium guttiforme, agent of the pineapple fusariosis, have led to the search for new methodologies, and for this we developed a new reliable molecular method to detect it. For diagnostic purposes, real-time PCR of elongation factor gene 1-α (ef1) was used to rapidly, specifically and sensitively diagnose F. guttiforme. A pathogenicity test was conducted with slips of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic; Diseases; Quarantine; Real-time PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100219

Use of food wastes for the production of lactic silage
Provedor de dados: 52
Autores: Martínez,Ricardo Murray; Mezquita,Pedro Cerezal; Bermúdez,Paulina; Muñoz,Richard Bugueño.
The goal of the present work was to produce lactic silage from food wastes. A factorial 2³ experimental design was applied using the following factors and levels: yogurt inoculum concentration (1 and 15%), sucrose (2 and 15%) and temperature (22 and 35 ºC) and as response variable, the soluble nitrogen content (SNC) at the end of the fermentation was considered. The best SNC output was for the treatment with 1 % of inoculum, 2 % of sucrose and temperature of 22ºC. The increase of SNC with regards to its initial content, from 0.17 % to 1.67 % for protein contents (PC) &lt; 5 % corresponded to 882 % and from 0.65 % to 2.36 % for PC &amp;gt; 5 % represented 263 %. It was possible to produce a lactic silage and keep it stable for up to 30 days, which was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food waste; Lactic silage; Lactic fermentation; Institutional feeding.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100015

Morphological study of biomass during the start-up period of a fixed-bed anaerobic reactor treating domestic sewage
Provedor de dados: 52
Autores: Lima,Cláudio Antonio Andrade; Ribeiro,Rogers; Foresti,Eugenio; Zaiat,Marcelo.
This work focused on a morphological study of the microorganisms attached to polyurethane foam matrices in a horizontal-flow anaerobic immobilized biomass (HAIB) reactor treating domestic sewage. The experiments consisted of monitoring the biomass colonization process of foam matrices in terms of the amount of retained biomass and the morphological characteristics of the cells attached to the support during the start-up period. Non-fluorescent rods and cocci were found to predominate in the process of attachment to the polyurethane foam surface. From the 10th week of operation onwards, an increase was observed in the morphological diversity, mainly due to rods, cocci, and Methanosaeta-like archaeal cells. Hydrodynamic problems, such as bed clogging and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaerobic digestion; Biofilm; Domestic sewage; Fixed-bed reactor; Polyurethane foam.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600020

Species of Lepidoptera defoliators of Eucalyptus as new host for the parasitoid Palmistichus elaeisis (Hymenoptera: Eulophidae)
Provedor de dados: 52
Autores: Pereira,Fabricio Fagundes; Zanuncio,Teresinha Vinha; Zanuncio,José Cola; Pratissoli,Dirceu; Tavares,Marcelo Teixeira.
Pupae of Thyrinteina arnobia (Stoll) and Thyrinteina leucoceraea Rindge (Lepidoptera: Geometridae) were obtained from Eucalyptus cloeziana F. Muell and Eucalyptus urophylla S.T. Blake plants, respectively. Specimens of a parasitoid emerged from T. arnobia pupae and also found parasitising T. leucoceraea pupae in the field were identified as Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae). This is the first report on P. elaeisis parasitizing T. arnobia and T. leucoceraea pupae in natural conditions in Brazil. P. elaeisis also parasitized these hosts and Bombyx mori Linnaeus (Lepidoptera: Bombycidae), Anticarsia gemmatalis Hubner, Pseudaletia sequax Franclemont, Alabama argillacea Huebner (Lepidoptera: Noctuidae), Dirphia moderata Bouvier...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Lepidopterous; Parasitoid.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200004

Internal exposure in nuclear medicine: application of IAEA criteria to determine the need for internal monitoring
Provedor de dados: 52
Autores: Dantas,Bernardo Maranhão; Lucena,Eder Augusto de; Dantas,Ana Letícia Almeida.
The manipulation of unsealed sources in nuclear medicine poses significant risks of internal exposure to the staff. According to the International Atomic Energy Agency, the radiological protection program should include an evaluation of such risks and an individual monitoring plan, assuring acceptable radiological safety conditions in the workplace. The IAEA Safety Guide RS-G-1.2 recommends that occupational monitoring should be implemented whenever it is likely that committed effective doses from annual intakes of radionuclides would exceed 1 mSv. It also suggests a mathematical criterion to determine the need to implement internal monitoring. This paper presents a simulation of the IAEA criteria applied to commonly used radionuclides in nuclear medicine,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear medicine; Internal monitoring; Radiation protection.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700017

Modifications on leaf anatomy of Coffea arabica caused by shade of pigeonpea (Cajanus cajan)
Provedor de dados: 52
Autores: Morais,Heverly; Medri,Moacyr Eurípedes; Marur,Celso Jamil; Caramori,Paulo Henrique; Ribeiro,Ana Maria de Arruda; Gomes,José Carlos.
Modifications on leaf anatomy in Coffea arabica shaded with pigeonpea (Cajanus cajan), compared to cultivation under full sun, were assessed. The leaves fully exposed to sunlight presented thicker cuticles and cellular walls, narrower epidermis cells, palisade parenchyma with longer cells, thicker lacunar parenchyma, fewer intercellular spaces and a larger stomata number. Leaves under dense shade presented a narrower cuticle and cellular wall; a mesophyll with smaller volume, but with larger intercellular spaces; and epidermis with thicker cells and a smaller stomata amount, surrounded by subsidiary cells of smaller dimensions. Plants grown under full sunlight presented higher values of net photosynthesis. The results evidenced that the species C. arabica...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Shading; Morphology; Physiology; Agroforestry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600005

Insecticide distribution model in human tissues viewing worker's health monitoring programs
Provedor de dados: 52
Autores: Paraíba,Lourival Costa; Castro,Vera Lúcia Scherholz Salgado de; Maia,Aline de Holanda Nunes.
This work aimed at evaluating the characteristics of thirty nine insecticides on tissue distribution and accumulation, using their physico-chemical characteristics and the tissues lipid contents to calculate the compounds distribution among the tissues. The insecticides evaluated were selected among those registered in Brazil for agriculture use. The level I fugacity model was used for the calculations of insecticide distribution among the tissues of muscles, viscera, skin, fat, blood, liver, kidneys and gut. The octanol-water partition coefficient, water solubility and tissue lipid contents showed an insecticide distribution in human tissues. Cluster analysis was performed aiming the identification and separation of insecticides groups based on their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fugacity model; Insecticide tissue distribution; Farmworkers; Health monitoring programs.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400011

Enteromorpha intestinalis Derived Seaweed Liquid Fertilizers as Prospective Biostimulant for Glycine max
Provedor de dados: 52
Autores: Mathur,Chetna; Rai,Saumya; Sase,Nikhil; Krish,Suthindhiran; Jayasri,Mangalam Achuthanandam.
ABSTRACT In the present study, the potential of seaweed liquid fertilizer (SLF) of marine algae Enteromorpha intestinalis was evaluated for its effect on seed germination, yield, biochemical parameters and pigment characteristics of Glycine maxE. intestinalis was collected form Mandapam coast of Gulf of Mannar, Tamil Nadu, and the dried seaweeds were used for the preparation of SLF. G. max seeds were germinated with four different concentrations (20, 40, 60, and 100%) of SLF; its growth and yield parameters were evaluated and compared with chemical fertilizer and control. The morphological and bio-chemical parameters such as seed germination (100%), root (6.6cm) and shoot length (5.4 cm), carbohydrates (0.098 mg/g), protein (0.56 mg/g), pigment (0.444 mg/g...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine algae; Enteromorpha intestinalis; Glycine max (L.) Merr; Seaweed liquid fertilizer; Plant growth.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600813

Mangrove bird community of Paranaguá Bay - Paraná, Brazil
Provedor de dados: 52
Autores: Mestre,Luiz Augusto Macedo; Krul,Ricardo; Moraes,Valéria dos Santos.
This study describes the mangrove bird community of Paranaguá Bay in Paraná - South Brazil. Seasonal surveys were conducted during September 1997 to September 1998 at three sites in Paranaguá Bay. Frequencies and abundances of birds were registered in 200m transects and one hour point counts. A total of 81 bird species were observed in the three sampling sites. Most of the bird species in Paranaguá mangroves are fruits, seeds and arthropods consumers, and predators of flying insects. The most frequent and abundant species were Egretta caerulea, Ceryle torquata, Chloroceryle amazona, Pitangus sulphuratus, Turdus amaurochalinus and Parula pitiayumi. The bird community of these three sites is composed mainly by forest bird species. The mangroves of Paranaguá...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangroves; Birds; Community; Paraná; Paranaguá - Bay.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100009

Influence of coffee pruning on the severity of frost damage
Provedor de dados: 52
Autores: Androcioli Filho,Armando; Caramori,Paulo Henrique.
Frost damages in a field experiment of pruning types and systems for the cultivars of Coffea arabica Catuaí and Mundo Novo, were evaluated at Londrina (23º22’S, 52º10´W), State of Parana, southern Brazil, during the winter of 1990 and 1994. Pruning types evaluated were ‘esqueletamento’ (cutting off all plagiotropic branches at 20-30 cm from the orthotropic branch), ‘decote’ (cutting off the orthotropic branch at 1.5 m and 2.0 m above ground) and ‘recepa’ (cutting off the orthotropic branch at 0.8 m above ground), performed on all rows and on alternate rows, and on different sections of the plant. Results indicated that frost damage could increase according to the type and height of pruning. The pruning type ‘esqueletamento’ and prunings at higher levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pruning; Coffea arabica; Coffee cultivars; Frost.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100006

Morphological and anatomical features of the flowers and fruits during the development of Chamissoa altissima (Jacq.) Kunth (Amaranthaceae)
Provedor de dados: 52
Autores: Oyama,Sayuri de Oliveira; Souza,Luiz Antonio de; Muneratto,Júnior Cesar; Albiero,Adriana Lenita Meyer.
The morphology and structure of the flowers and fruits of Chamissoa altissima (Jacq.) Kunth during the development were analyzed. The plant material was fixed in FAA 50, embedded in historesin and sectioned according to standard techniques. The flowers presented tepals with betalain and homogeneous mesophyll; receptacular nectary; anthers with epidermis, endothecium, one middle layer, and uninucleate tapetum; and monocarpelar ovary with homogeneous mesophyll. The fruit was a circumscissile capsule, presented the same number of cellular layers that the ovary, and possessed subendocarpic sclerenchyma. The seed was bitegmic, exotestal and perispermic.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Perianth; Stamen; Gynoecium; Pericarp; Seed.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600019

Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed
Provedor de dados: 52
Autores: Das,Mithu; Banerjee,Rintu; Bal,Satish.
The aim of this study was to evaluate the interaction effects of the physico-chemical parameters on the endoglucanase (CMCase) production by Trichoderma reesei Rut C30 on a cellulosic agro-residue by the solid-state fermentation (SSF) and to determine their optimum values by the EVOP factorial design technique. The best combination of physical parameters for the maximum production of the endoglucanase (CMCase) was 28ºC temperature, 79% relative humidity and 4.8 pH of the medium. The best combination of the chemical parameters was (mg/L) nicotinic acid 15, naphthalene acetic acid 7, ferric chloride 5 and Tween-80 6. With the application of this technique, the yield of the CMCase increased by ~ 2.3 fold.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Endoglucanase; Carboxymethylcellulase (CMCase); EVOP factorial design technique.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100005

Forest fragments' contribution to the natural biological control of Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) in Maize
Provedor de dados: 52
Autores: Sousa,Eduardo Henrique Santana; Matos,Michela Costa Batista; Almeida,Ruanno Silva; Teodoro,Adenir Vieira.
The present work evaluated the influence of forest distance on predatory solitary wasps' abundance and richness and its relation to the natural biological control of the fall armyworm (Spodoptera frugiperda Smith - Lepidoptera: Noctuidae) in maize. The study consisted of two parallel lines of six experimental maize plots located at an increasing distance from a forest edge. Four trap-nests were placed in the vertices of each plot of the first line to evaluate the abundance and species richness of predatory solitary wasps. The larvae of the fall armyworm were sampled weekly by inspecting ten randomly chosen plants per plot. Predatory solitary wasp abundance (but not species richness) decreased whilst the fall armyworm abundance increased with the distance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predatory solitary wasps; Fall armyworm; Ecosystem services; Natural biological control.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400015

Growth of Candida guilliermondii FTI 20037 on mixed substrate
Provedor de dados: 52
Autores: Gurgel,Patrick V.; Vieira,Délia C.; Furlan,Sandra A.; Mancilha,Ismael M. de.
Candida guilliermondii FTI 20037 was grown on a mixed substrate comprising glucose and xylose. Inocula were grown using xylose or glucose as carbon source. Results showed that xylose utilization was delayed until glucose was utilized. Inoculum prepared on glucose showed a lag phase in xylose consumption. Cell mass production was higher when glucose was utilized during fermentation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida guilliermondii; Glucose; Xylose; Mixed substrate.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300010

Measurements of the fallout flux of beryllium-7 and its variability in the soil
Provedor de dados: 52
Autores: Andrello,Avacir Casanova; Appoloni,Carlos Roberto.
The aim of this study was to examine the beryllium-7 behavior in the soil. Natural variability of beryllium-7 concentration was calculated to be about 23% (relative standard deviation), and the depth distribution could be approximated by an exponential decay in bare soil, with an average penetration depth in the soil about 1 cm. The nuclide was not found below 2 cm depth, which confirmed its utilization to infer the erosion processes as a tracer of soil surface. The maximum beryllium-7 concentration in the analyzed period was about 40 Bq.kg-1.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beryllium-7; Gamma ray spectrometry; Superficial soil erosion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100022

Biological characteristics of Trichogramma pretiosum and Trichogramma acacioi (Hym.: Trichogrammatidae), parasitoids of the avocado defoliator Nipteria panacea (Lep.: Geometridae), on eggs of Anagasta kuehniella (Lep.: Pyralidae)
Provedor de dados: 52
Autores: Pratissoli,Dirceu; Zanuncio,José Cola; Vianna,Ulysses Rodrigues; Andrade,Josimar Souza; Zanotti,Luiz Carlos Marozzi; Silva,Alexandre Faria da.
The objective of this investigation was to study Trichogramma pretiosum and T. acacioi (Hymenoptera: Trichogrammatidae), parasitoids of the avocado defoliator Nipteria panacea (Lepidoptera: Geometridae) on the eggs of the alternative host Anagasta kuehniella (Lepidoptera: Pyralidae) aiming to use them for biological control of this pest in avocado orchards. The cubic model presented better adjustment for duration of the life cycle of T. pretiosum and T. acacioi with the host A. kuehniella which shows that development rate of these species increases with temperature within the range tested. The number of individuals of both Trichogramma species emerged per egg from this host was higher than one. The quadratic model was significant for viability of T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avocado; Trichogramma; Alternative host; Mass rearing.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100002

Cyanogenic glycosides in plants
Provedor de dados: 52
Autores: Francisco,Ilza A.; Pinotti,Maria Helena Pimenta.
The presence of cyanogenic glycosides was determined in 70 plant species from the campus of the State University of Londrina, PR, Brazil, and a further 45 plant species from the Forestry Reserve on the Doralice Farm in Ibiporã, PR, Brazil. Of the vegetative species from the State University of Londrina, 7.1% showed cyanogenic glycosides: Manihot esculenta (Euphorbiaceae), Passiflora edulis (Passifloraceae), Macadamia ternifolia (Proteaceae), Prunus persica (Rosaceae) and Beloperone sp (Acanthaceae).The first four species were considered to be potentially cyanogenic in the field. From the Forestry Reserve on the Doralice Farm, the plant species with cyanogenic glycosides were: Holocalix balanseae (Caesalpinaceae), Nectranda megapotamica (Lauraceae),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyanogenic glycosides; Cyanogenic plants; Cyanogenesis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500007

Wild boars (Sus scrofa scrofa) seminiferous tubules morphometry
Provedor de dados: 52
Autores: Costa,Deiler Sampaio; Silva,José Frederico Straggiotti.
The aim of this data was to analyze morphology and function of the seminiferous tubule in adult wild boars. Testes removed by unilateral castration of five animals were used. The testicular parenchyma was composed by 82.1±2.2% of seminiferous tubule and 17.9±2.2% of intertubular tissue. The tubular diameter was 249.2±33.0 µm and the seminiferous tubule lenght per gram of testis was 19.3±4.9m. The spermatogonial mitoses efficiency coefficient, meiotic index and spermatogenesis efficiency were 10.34, 2.71 and 30.5 respectively. Each Sertoli cell supported about 13 germinatives cells. The hystometric parameters studied were very similar to those related for domestic boars, however, the wild boars intrinsic efficiency of spermatogenesis and Sertoli cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seminiferous tubule; Wild boar; Sus scrofa scrofa.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600008

Sodium fluoride does not alter sperm production or sperm morphology in rats
Provedor de dados: 52
Autores: Chioca,Lea Rosa; Müller,Juliane Centeno; Boareto,Ana Cláudia; Andreatini,Roberto; Dalsenter,Paulo Roberto.
The aim of the present study was to evaluate the effects of sodium fluoride (NaF) on the male reproductive system. Adult male rats were exposed to NaF in drinking water for 30 days at three concentrations: 1.54 (control, tap water), 50 and 100 ppm. Body and organ weights, daily sperm production, sperm number and morphology were investigated. No difference was observed on the sperm number and morphology among the groups, as well as body weight and organ absolute and relative weights. Overall, despite the presence of a mild degree of dental fluorosis in the higher dose group, the results indicated that exposure to NaF at the doses used in the present study did not adversely affect sperm production and morphology of male rats.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoride; Sodium fluoride; Male rats; Reproductive toxicology; Dental fluorosis; Sperm.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200011

Nutrients behavior from the association pig slurry and chemical fertilizers on soybean crop
Provedor de dados: 52
Autores: Smanhotto,Adriana; Sampaio,Silvio César; Bosco,Tatiane Cristina Dal; Prior,Maritane; Soncela,Rosimaldo.
This study aimed at evaluating the effects of pig slurry application with mineral fertilizer on ions leaching from the soil in soybean crop. The experiment was carried out in 24 drainage lysimeters under protection. The soybean cultivar CD 202/COODETEC was sown in a soil that received 0, 100, 200 and 300 m³ha-1of pig slurry in one cycle, with or without mineral fertilizer. There were three samplings of soil throughout the trial to determine the pH, N, NO3-, K+, Ca2+, Mg2+, Na+, Cu+2, Zn+2, OM, CEC, EC and SAR six times during the crop cycle. The yield was determined in the plants. In soil, pig slurry increased the concentrations of pH, NO3-, K+, Zn+2, OM and CEC, while mineral fertilizer increased P and Zn+2concentrations. The limits observed for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic fertilization; Soil pollution; Environment contamination; Soybean culture.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500003

Anatomy of somatic embryogenesis in Carica papaya L.
Provedor de dados: 52
Autores: Fernando,Juliana A.; Melo,Murilo; Soares,Marli K. M.; Appezzato-da-Glória,Beatriz.
Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Somatic embryogenesis; Papaya.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005

Chemical and nutritional composition of some common foods in Abeokuta, Nigeria
Provedor de dados: 52
Autores: Sanni,Silifat Ajoke; Oguntona,Clara Raquel Bernstein; Sanni,Lateef Oladimeji.
Chemical composition of seven common foods viz. cooked rice, cooked beans, cooked yams, cooked fufu, fried fish, stew, and vegetable soup, sold by the selected (147) vendors in Abeokut, were investigated. Descriptive statistics was used to analyse the collected data. The results of the proximate analysis of food samples showed that the mean moisture content of the street foods ranged from 44.87% in fried fish to 72.43% in cooked fufu. Protein content varied from 0.03% in cooked fufu to 14.47% in fried fish while the fat content ranged from 0.03% in cooked fufu to 34.43% in fried fish. Ash and carbohydrate contents ranged from 0.42% in cooked rice to 16.07% in vegetable soup and 0.73% in stew to 29.27% in cooked rice, respectively. The energy content ranged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical and nutritional composition; Common foods; Abeokuta; Nigeria.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300010

Protein Expression and Codon 72 Polymorphism of TP53 Gene in Triple Negative Breast Cancer
Provedor de dados: 52
Autores: Lopes,Leandra Fiori; Guembarovski,Roberta Losi; Guembarovski,Alda Losi; Kishima,Marina Okuyama; Campos,Clodoaldo Zago; Derossi,Daniela Rudgeri; Ariza,Carolina Batista; Ozawa,Patricia Midori Murobushi; Oliveira,Carlos Eduardo Coral de; Banin-Hirata,Bruna Karina; Vitiello,Glauco Akelinghton Freire; Borelli,Sueli Donizete; Watanabe,Maria Angelica Ehara.
A subgroup of tumor that has received attention is triple-negative breast cancer (TNBC), which presents phenotype of negative estrogen receptor, negative progesterone receptor and has no overexpression of HER2. TP53 acts as a tumor suppressor limiting the proliferation of damaged cells. A polymorphic site (rs1042522) of TP53 encodes either an arginine or a proline amino acid, but its biological significance remains unclear. This study aimed to investigate this variant and its expression in search for a possible involvement in TNBC susceptibility and clinical outcome. Genetic polymorphism was evaluated in 50 patients and 115 controls by PCR based methodology and immunohistochemistry was done with monoclonal antibody. Case-control study showed no positive or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; TNBC; TP53; Genetic polymorphism; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600895

Immobilization of alpha-amylase produced by Bacillus circulans GRS 313
Provedor de dados: 52
Autores: Dey,Gargi; Bhupinder,Singh; Banerjee,Rintu.
A maltooligosaccharide-forming amylase from B circulans GRS 313 was immobilized by entrapment in calcium alginate beads. The immobilized activity was affected by the size of the bead and bead size of 2mm was found to be most effective for hydrolysis. Kinetics constants, Km and Vmax were estimated and were found to be affected by the bead size. The catalytic activity of the enzyme was studied in presence of various starchy residues and metal ions. HgCl2, CuSO4 and FeCl3 caused inhibition of the enzyme. The reaction conditions, pH and temperature, was optimized using response surface methodology. At the optimum pH and temperature of 4.9 and 57ºC, the apparent activity was 25.6U/g of beads, resulting in almost 2-fold increase in activity. The immobilized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus circulans GRS313; Entrapment; Maltooligosaccharide-forming amylase; Response surface methodology; Starchy residues.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200005

Pollination and dispersal systems in a Cerrado remnant (Brazilian Savanna) in Southeastern Brazil
Provedor de dados: 52
Autores: Ishara,Katia Losano; Maimoni-Rodella,Rita de Cassia Sindrônia.
The aim of this work was to identify the pollination and dispersal systems among the species in a disjunct marginal cerrado area and to compare the frequency of these systems to those found in other Neotropical vegetation. The floral and diaspore traits and the pollination and dispersal systems of 176 species were analysed in a cerrado remnant in Southeastern Brazil. The most frequent pollination system was melittophily (63% of the studied species) with the remaining 37% distributed among diverse pollination systems. Zoochory was the predominant system of dispersal (44.9%). The frequencies of melittophily and zoochory observed in diverse tropical areas were the main feature that allowed the formation of distinct groups in the dendrograms generated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dispersal syndrome; Melittophily; Pollination syndrome; Similarity; Zoochory.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300025

Food extraction by the males of Podisus nigrispinus (Dallas) (Hemiptera: Pentatomidae) from cotton leafworm larvae
Provedor de dados: 52
Autores: Pereira,Alexandre Igor de Azevedo; Ramalho,Francisco de Sousa; Rodrigues,Karjoene Cassimiro Vilar; Malaquias,José Bruno; Souza,Jefferson Virgínio da Silva; Zanuncio,José Cola.
In this work, the effect of different densities (1, 3, 5, 7 and 9) of 3rd instar Alabama argillacea (Huebner) larvae on food consumption by Podisus nigrispinus (Dallas) males was evaluated. The densities established were converted to weight of prey offered: 13.4 mg (one larva), 33.3 mg (three larvae), 54.3 mg (five larvae), 81.8 mg (seven larvae), and 110.34 mg (nine larvae). The quantity of food consumed by P. nigrispinus increased with the prey density. The density of preys did not affect the time spent by the predator to ingest the food. The quantity of food extracted per minute was always higher in smaller densities and lower in higher densities. Males mean body weight did not differ statistically between the treatments tested, and weight gain was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotton leafworm; Predator; Stinkbug; Food consumption; Nutrition.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500004

Parameters for dimensional inspection of orifice plates and roughness of the straight stretches of the tubing
Provedor de dados: 52
Autores: Medeiros,Alberto Kennedy de Almeida; Lima,Jeffersson Fernandes de; Medeiros,Gilson Gomes de; Silva Junior,Nivaldo Ferreira da; Felipe,Raimundo Nonato B.; Felipe,Renata Carla Tavares dos Santos.
According to the Technical Regulation of Measurement of Petroleum and Natural Gas (approved in 2000 by ANP - the Petroleum National Agency of Brazil), the systems of measurement of natural gas should use ultra-sonic flow meters, turbines and, especially, the orifice plates, which represent the most expressive base of the flow systems, being used in the fiscal and operational measurements. This work aims to evaluate the parameters that should be accounted for during the dimensional inspection of orifice plate and roughness of the straight stretches of the tubing used for measurement of natural gas, with the objective of guiding the responsible professionals for the measurement of volumes of natural gas by orifice plate, as well as the professionals of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orifice plate; Superficial roughness; Measurement parameters.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200002

Evaluation of the Heat and Energy Performance of a Datacenter Using a New Efficiency Index: Energy Usage Effectiveness Design – EUED.
Provedor de dados: 52
Autores: Santos,Alexandre F.; Gaspar,Pedro D.; Souza,Heraldo J.L. de.
Abstract Data Centers are growing steadily worldwide, and they are expected to continue growing up to 53% in 2020. Energy efficiency, in high power consumption, is a key venue. There are methodologies to measure this efficiency, one example is using the PUE (Power Usage Effectiveness) index. In this paper is proposed a new index for measuring efficiency at the design stage, the EUED (Energy Usage Effectiveness Design). This index allows to evaluate systems using “free cooling” and adiabatic system. A comparison is performed considering the equipment in the worst situation. The thermodynamics parameter enthalpy is used to calculate the results. This new methodology allows to determine differences between the cities of São Paulo and Curitiba (1.21%) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Data Centers; PUE; EUED; Performance indexes; Data Center Cooling.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200220

Performance of calves submitted to protocols using extruded or ground starter
Provedor de dados: 52
Autores: Chaves,Amália Saturnino; Costa,Suely de Fátima; Melo,Leandra Queiroz de; Pereira,Marcos Neves.
Feed processing can affect rumen development in new born calves, and potentially define animal performance. Two feed management systems, extruded starter (Ruter) with possible early weaning and ground starter (control), were evaluated in thirty-two Holstein calves (16 females and 16 males). Animals were randomly assigned to the treatments using a randomized block design with birth weight as a covariate. They were weaned when starter intake reached 800 g for two consecutive days. Twenty-one days after the weaning, males were slaughtered and the stomach compartments were isolated. Rumen and omasum fragments were processed for morphological evaluation. Animal performance, clinical condition and stomach compartment weight did not differ between the treatments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk-feeding period; Grain processing; Reticulorumen.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500695

Ionically Bound Peroxidase from Peach Fruit
Provedor de dados: 52
Autores: Neves,Valdir Augusto.
Soluble, ionically bound peroxidase (POD) and polyphenoloxidase (PPO) were extracted from the pulp of peach fruit during ripening at 20°C. Ionically bound form was purified 6.1-fold by DEAE-cellulose and Sephadex G-100 chromatography. The purified enzyme showed only one peak of activity on Sephadex G-100 and PAGE revealed that the enzyme was purified by the procedures adopted. The purified enzyme showed a molecular weight of 29000 Da, maximum activity at pH 5.0 and at 40ºC. The calculated apparent activation energy (Ea) for the reaction was10.04 kcal/mol. The enzyme was heat-labile in the temperature range of 60 to 75ºC with a fast inactivation at 75ºC. Measurement of residual activity showed a stabilizing effect of sucrose at various temperature/sugar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peach peroxidases; Ripening; Purification; Kinetics; Heat stability.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100002

Endophytes: exploitation as a tool in plant protection
Provedor de dados: 52
Autores: Dutta,Devanushi; Puzari,Keshab Chandra; Gogoi,Robin; Dutta,Pranab.
Endophytes are symptomless fungal or bacterial microorganisms found in almost all living plant species reported so far. They are the plant-associated microbes that form symbiotic association with their host plants by colonizing the internal tissues, which has made them valuable for agriculture as a tool in improving crop performance. Many fungal endophytes produce secondary metabolites such as auxin, gibberellin etc that helps in growth and development of the host plant. Some of these compounds are antibiotics having antifungal, antibacterial and insecticidal properties, which strongly inhibit the growth of other microorganisms, including plant pathogens. This article reviews the endophyte isolated from different plants, mode of endophytic infection and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics; Auxin; Endophytism; Insecticidal; Symbiotic.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500621

Pretreatment strategies for delignification of sugarcane bagasse: a review
Provedor de dados: 52
Autores: Karp,Susan Grace; Woiciechowski,Adenise Lorenci; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo.
The valorization of agro-residues by biological routes is a key technology that contributes to the development of sustainable processes and the generation of value-added products. Sugarcane bagasse is an agro-residue generated by the sugar and alcohol industry in Brazil (186 million tons per year), composed essentially of cellulose (32-44%), hemicellulose (27-32%) and lignin (19-24%). The conversion of sugarcane bagasse into fermentable sugars requires essentially two steps: pretreatment and hydrolysis. The aim of the pretreatment is to separate the lignin and break the structure of lignocellulose, and it is one of the most critical steps in the process of converting biomass to fermentable sugars. The aim of this review is to describe different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane Bagasse; Lignocellulose; Pretreatment; Delignification.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400019

Genetic variability in regenerated Metarhizium flavoviride protoplasts
Provedor de dados: 52
Autores: Kuklinsky-Sobral,Júlia; Luna-Alves-Lima,Elza Áurea de; Araújo,Janete Magali de; Azevedo,João Lúcio.
Protoplast isolation and regeneration were evaluated in two wild-type and two colour mutant strains of Metarhizium flavoviride. Cultivation in liquid medium, followed by mycelium treatment with Novozym 234 in the presence of KCl 0.7M as osmotic stabilizer, produced 5.05 x 10(6) to 1.15 x 10(7)x mL-1 protoplasts. The percentage of regeneration ranged from 6.65 to 27.92%. Following protoplast regeneration, one strain produced spontaneously stable morphological variant colonies. Although colonies with altered morphology have been reported in bacteria following protoplast regeneration, this is the first time that the same is described in a filamentous fungus. The original strain and one derived variant were tested for sensitivity to the fungicides benomyl and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium flavoviride; Protoplasts; Genetic variability; Benomyl; Captan.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100001

Solid-state fermentation of Acanthogobius hastaprocessing by-products for the production of antioxidant protein hydrolysates with Aspergillus oryzae
Provedor de dados: 52
Autores: Fang,Yaowei; Wang,Shujun; Liu,Shu; Lu,Mingsheng; Jiao,Yuliang; Chen,Guoqiang; Pan,Jianmei.
Functional properties and antioxidative activity of a protein hydrolysate prepared from Acanthogobius hasta processing by-product protein during solid-state fermentation with Aspergillus oryzae were investigated. Overall, protease activity increased with the degree of hydrolysis (DH) decreased during solid-state fermentation. All the protein hydrolysate had excellent solubility, possessed interfacial properties, and varying degrees of antioxidant activity which were governed by their concentrations and DH, molecular weight distribution and amino acid composition. After 5 days fermentation, the DH of the protein hydrolysate was 31.23%. The protein hydrolysate had the highest total hydrophobic amino acid content, the highest DPPH scavenging activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functionalities and antioxidant properties; Acanthogobius hasta processing by-product; Solid state fermentation; Protein hydrolysates; Aspergillus oryzae.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300343

Physical characteristics of Brazilian wheat and their utilization as an indication of flour extraction
Provedor de dados: 52
Autores: Germani,Rogério; Carvalho,José Luiz Viana de.
An extensive work to determine the test weight (TW), the thousand kernel weight (TKW) and the hardness (HD) of Brazilian wheat, was undertaken for the crop years from 1990 to 1994, with the objetive of drawing the Brazilian wheat physical characteristics and their relationship with flour extraction (FE). The results showed a median TW of 78.2kg/hl, a TKW of 34.2g and the HD was graded as hard. The values varied accordingly with the crop year, the local and year vs. local. Among the locals the wheat from the MS/DF/MG/GO States showed the best results. Correlation coefficients between FE and each of the parameters, TW, TKW and HD were low but significant (P&lt; 5.0%) and, therefore, should not be used as a direct relationship but only as an indicator of FE....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Flour extraction; Hectoliter weight; Thousand kernel weight; Hardness.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100004

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
Provedor de dados: 52
Autores: Yan,Xu; Souza,Maria Aparecida; Pontes,Marcela Z. R.; Vitolo,Michele; Pessoa Júnior,Adalberto.
From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Affinity partitioning; Aqueous two-phase systems; Reactive dyes; Ligands; Enzymes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030

Personalized Gene Expression Analyses of SMAD7 and KLF10 In Breast Cancer
Provedor de dados: 52
Autores: Hosseinzadeh,Ali; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Seifi,Morteza; Gavgani,Reyhaneh Ravanbakhsh; Nadergoli,Omid Kheyri; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim.
Many cancer researchers use gene expression analysis for differentiation between tumor and normal cells for diagnostic, prognostic and therapeutic purposes. Most of studies compare either tumor cell lines by normal cell lines or tumor tissue of affected individuals by normal healthy control tissue. But expression of each special gene is unique in different individuals and also in different tissue of same individual. For this reason, here we compare the gene expression levels of SMAD7 and KLF10 in tumor cells and its adjacent normal tissue of breast cancer patients and compared them. For this purpose, a total of 40 tumor and matched tumor-free margin samples were obtained during surgery. The SMAD7 and KLF10 mRNA expression levels in tumor and marginal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: KLF10; Personalized gene Expression; Real-time PCR; SMAD7; TGFβ.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100317

A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery
Provedor de dados: 52
Autores: Severo Jr.,João Batista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BSA; Protein adsorption; Breakthrough curves; Amberlite IRA 410 ion-exchange resin; Distribution residence time study; Richardson-Zack coefficient.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200021

Effect of eugenol on growth and listeriolysin o production by Listeria monocytogenes
Provedor de dados: 52
Autores: Filgueiras,Cristina Tostes; Vanetti,Maria Cristina Dantas.
The inhibitory effect of eugenol, a naturally occurring compound mainly present in the essential oil fraction of cloves, was studied on the growth and listeriolysin O (LLO) production by Listeria monocytogenes. Potassium efflux from cells promoted by eugenol was also determined after 24 h incubation in phosphate buffered saline. Eugenol promoted a delay on the growth of L. monocytogenes at concentrations of 100, 300 and 500 µg mL-1and above 800 µg mL-1 the effect was bactericidal. Production of LLO by L. monocytogenes in the presence of eugenol was reduced 80-100%. An accumulation of external K+ was observed above 300 µg mL-1 of eugenol which indicated that the cell membrane was affected. The results showed the effectiveness of eugenol in controlling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenol; Listeria monocytogenes; Antilisteric; LLO.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400008

Cloning of a peroxidase gene from cassava with potential as a molecular marker for resistance to bacterial blight
Provedor de dados: 52
Autores: Pereira,Luiz Filipe; Goodwin,Paul H.; Erickson,Larry.
Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis, is considered one of the most important bacterial diseases of cassava (Manihot esculenta Crantz). In order to characterize the cassava genes involved in resistance to this disease, a genomic clone of a cationic peroxidase gene, MEPX1, was isolated by PCR from cassava cultivar MCOL 22. The DNA sequence of MEPX1 showed high homology with other plant peroxidase genes and contained a large intron typical of peroxidase genes. The predicted translation product showed a heme-ligand motif, also a characteristic of peroxidases, as well as phosphorylation, myristoylation and glycosylation sites. The amino acid sequence had 75 % homology with two Arabidopsis thaliana peroxidases. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Peroxidase; Bacterial blight; Xanthomonas axonopodis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200002

Biodistribution of the radiopharmaceutical technetium-99m-sodium phytate in rats after splenectomy
Provedor de dados: 52
Autores: Pereira,Kércia Regina Santos Gomes; Açucena,Maria Kadja Meneses Torres; Villarim Neto,Arthur; Rêgo,Amália Cínthia Meneses; Bernardo-Filho,Mário; Azevedo,Ítalo Medeiros; Araújo Filho,Irami; Medeiros,Aldo Cunha.
Drugs and surgery can interfere with the biodistribution of radiopharmaceuticals and data about the effect of splenectomy on the metabolism of phytate-Tc-99m are scarce. This study aimed at evaluating the interference of splenectomy on phytate-Tc-99m biodistribution and liver function in rats. The SP group rats (n=6) underwent splenectomy. In group C (control) the animals were not operated on. After 15 days, all rats were injected with 0.1mL of Tc-99m-phytate via orbital plexus (0.66MBq). After 30 minutes, liver samples were harvested, weighed and the percentage of radioactivity per gram (%ATI/g) was determined by a Wizard Perkin-Elme gama counter. The ATI%/g in splenectomized rats (0.99±0.02) was significantly higher than in controls (0.4±0.02),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Splenectomy; Sodium phytate; Biodistribution; Liver.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700033

Methodology adjustments for organic acid tolerance studies in oat under hydroponic systems
Provedor de dados: 52
Autores: Kopp,Mauricio Marini; Luz,Viviane Kopp da; Souza,Velci Queiróz de; Coimbra,Jefferson Luis Meirelles; Sousa,Rogério Oliveira de; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de.
The occurrence of anaerobic conditions in hydromorphic soils favors the development of anaerobic microorganisms that produce phytotoxic substances representing primarily by organic acids. The selection of promising oat (Avena sativa L.) genotypes for use in those situations requires field evaluations that can be cumbersome, making hydroponics a viable alternative. The objective of this work was to adjust a methodology to use in studies of tolerance to organic acids in oat under hydroponic systems. For such goal, the best germination system was determined in order to reduce the seedling initial establishment effects under hydroponics, the ideal concentration for screening genotypes and the best variable for stress evaluation. It was found that the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytotoxicity; Levels; Abiotic stress; Genetic breeding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300003

Diet, Epidemiological Factors and Cognitive Impairment: A Cross-Sectional Study in the Elderly Population
Provedor de dados: 52
Autores: França,Vivian Francielle; Azzolini,Thairine; Pissaia,Ediane; Bortoloti,Durcelina Schiavoni; Signorini,Taise; Costa,Lediana Dalla; Souza,Marília Moraes Queiroz; Lívero,Francislaine Aparecida dos Reis; Lovato,Evellyn Claudia Wietzikoski.
ABSTRACT Purpose Population aging is one of the great achievements of humanity. However, with aging, the incidence of chronic diseases and those related to cognition deficits increase. In this way, strategies aimed at preventing or delaying cognitive deficit are extremely necessary. Thus, this study investigates the relationship between cognitive deficits, schooling and eating habits in a Brazilian elderly population. Methods The dietary habits of 400 older adults were investigated through structured questionnaires. Unhealthy eating habits such as low consumption of fruits, vegetables and beans, consumption of fatty meats and whole milk, adjusted for other variables, were evaluated. Cognitive function was assessed by the Mini Mental State Examination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Cognitive; Diet; Fruits; Schooling; Vegetable.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100327

Tolerance of arabica coffee cultivars for aluminum in nutritive solution
Provedor de dados: 52
Autores: Macedo,Célia Maria Peixoto de; Lopes,José Carlos; Amaral,José Augusto Teixeira do; Fonseca,Aymbiré Francisco Almeida da; Amaral,José Francisco Teixeira do.
This work aimed to evaluate the Coffea arabica cultivars for aluminum toxicity tolerance, in modified Hoagland solution. A completely randomized design with five repetitions in a factorial 4 x 4 (cultivar x combinations of aluminum) was used. After 44 days of the sowing, were transferred ten seedlings each cultivar germinated in the absence of Al3+ to solution without Al3+, and ten for solution with Al3+; ten seedlings each cultivar germinated in presence of Al3+ to solution without Al3+, and ten for solution with Al3+. In the treatment with aluminum, the element was added to the nutritive solution in the concentration of 0.83 mmol L-1 as Al2(SO4)3.16H2O. The cultivars Catuaí Amarelo IAC 62 and Iapar 59 were tolerant to the aluminum; cultivar Oeiras...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Aluminum toxicity; Vigor; Breeding.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500005

Investigation of Mode of Action of Anti Bacterial Activity of Salacia Oblonga Extract Against Drug Resistant Pathogen
Provedor de dados: 52
Autores: Musini,Anjaneyulu; Giri,Archana.
Abstract Salacia oblonga Wall belonging to family Celastraceae contains vital phytoconstituents and has been used since long for the treatment of diabetes, inflammation and burn wounds. S. oblonga ethyl acetate root extract was evaluated for antibacterial activity towards drug resistant pathogens Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Further 260 nm absorbing material was estimated in the control and treated cells. Interestingly 260 nm absorbance material is higher in the Staphylococcus aureus. Further the effect of the plant extract on drug resistant pathogen S. aureus was examined by scanning electron microscopy (SEM). SEM results have shown that treated bacterial cells have changed in morphology, size and reduced in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salacia oblonga; Antibacterial activity; Drug resistant pathogen; And SEM.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100417

Application of xylanases from Amazon Forest fungal species in bleaching of eucalyptus kraft pulps
Provedor de dados: 52
Autores: Medeiros,Roseli Garcia; Silva Jr.,Francides Gomes da; Báo,Sônia Nair; Hanada,Rogério; Ferreira Filho,Edivaldo Ximenes.
Crude xylanase preparations from Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum were used to treat Eucalyptus kraft pulp, prior to chlorine dioxide and alkaline bleaching sequences. The enzyme pretreatment improved brightness and delignification of non-delignified and oxygen-bleached samples of eucalyptus kraft pulp. Xylanase preparations from T. longibrachiatum and P. corylophilum were more effective to reduce pulp kappa number. A small reduction in viscosity was obtained when the oxygen-bleached pulp was treated with xylanase preparation from A. niger. For all enzyme samples, the best release of chromophoric material from the pulp was at 237 nm. The enzyme preparation from P. corylophilum was responsible for the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylan; Xylanase; Kraft pulp; Pulp bleaching.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200008

Effects of Cinnamomum zeylanicum treatment on radiolabeling of blood bonstituents and morphology of red blood cells in Wistar rats
Provedor de dados: 52
Autores: Benarroz,Mônica Oliveira; Rocha,Gabrielle de Souza; Pereira,Márcia Oliveira; Geller,Mauro; Fonseca,Adenilson de Souza da; Presta,Giuseppe Antonio; Bernardo-Filho,Mario.
The aim of this study was to evaluate the effect of in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. Animals were treated with cinnamon extract at different doses and for different periods of time. As controls, animals treated with 0.9% NaCl. Labeling of blood constituents with 99mTc was performed. Plasma, blood cells and insoluble fractions were isolated. Radioactivity in each fraction was counted and the percentage of radioactivity (%ATI) was calculated. Also, blood smears were prepared to morphological analysis of red blood cells from. Data showed that in vivo cinnamon extract did not significantly (p&gt;0.05) modify the %ATI of blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood constituents; Cinnamomum zeylanicum; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700023

Improving Production of Tacrolimus In Streptomyces Tacrolimicus (ATCC 55098) Through Development of Novel Mutant by Dual Mutagenesis
Provedor de dados: 52
Autores: Singh,Bhanu P; Kumar,Punit; Haque,Shafiul; Jawed,Arshad; Dubey,Kashyap Kumar.
ABSTRACT Tacrolimus is a polyketide macrolide produced by Streptomyces species which is widely used as anti-fibrotic agent and potent immunosuppressant. In this article dual mutagenesis approach using mutagens (NTG+EMS+UV) was used to develop a mutant strain of Streptomyces tacrolimicus (ATCC 55098) for higher tacrolimus production and this strain showed higher tacrolimus production at 82.5 mg/l. Interestingly; addition of L-Lysine (0.2 g/l) into the production medium further enhanced the tacrolimus production to ~102 mg/l at 7-L fed-batch bioreactor. To the best of our knowledge this is the first report mentioning efficient strain development for higher production of tacrolimus using dual mutagenesis. The obtained data presents an impressive model for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunosuppressant; Macrolide polyketide; Tacrolimus; Mutagenesis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100419

The influence of starvation and Eurytrema coelomaticum infection on the nitrogenous products of degradation in the hemolymph of Bradybaena similaris
Provedor de dados: 52
Autores: Souza,Rosana M. de; Gomes,Edna M.; Chagas,Generoso M.; Pinheiro,Jairo.
The urea and uric acid contents in the hemolymph of Bradybaena similaris were analyzed under starvation and Eurytrema coelomaticum infection during 30 days. The urea and uric acid contents in the uninfected and fed snails were 15.090 mg/dl and 9.399 mg/dl, respectively, being the urea/uric acid ratio 1.6. The urea content in starved snails increased to a maximum of 363.237 mg/dl at 15 days, and the uric acid varied to a maximum of 11.470 mg/dl. The urea content in infected snails was reduced to a minimum of 2.123 mg/dl at 30 days of infection and the uric acid increased significatively at 25 days post exposure, being 13.820 mg/dl. A significative relation was not observed between the time of starvation and time of E. coelomaticum infection and the uric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urea; Uric acid; Starvation; Bradybaena similaris; Eurytrema coelomaticum; Host-parasite relationship.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400003

Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
Provedor de dados: 52
Autores: Goulart,Antonio José; Carmona,Eleonora Cano; Monti,Rubens.
Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45º C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 µmol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL) than at pH 9.0.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaline xylanase; Rhizopus stolonifer; Solid-state fermentation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300001

Reproductive biology of Bryconamericus stramineus Eigenmann, 1908 (Ostariophysi: Characidae) from the Rio Ibicuí, RS, Brazil
Provedor de dados: 52
Autores: Lampert,Vinicius Renner; Azevedo,Marco Aurélio; Fialho,Clarice Bernhardt.
Reproduction of Bryconamericus stramineus was described based on the analysis of 208 females and 166 males collected monthly in the Rio Ibicuí, from April 2001 through March 2002. Reproduction occurred between September and December, with a lower peak in February. The mean absolute fecundity was 371.3 (± 244.6) oocytes and the mean relative fecundity was 0.35 (± 0.08) oocytes/mg of total weight and the fish was a multiple spawner. A weak correlation between GSI of males and day length was observed. Other parameters (stomach repletion and hepatosomatic index, rainfall and temperature) did not show any correlation with GSI. However, some of these factors might act as "starters" of gonadal maturation. The presence and frequency of fin ray hooks on the males...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproductive period; Fecundity; Spawning type; Bryconamericus; Fin-ray hooks.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700011

Characterization of açaí (E. oleracea) fruits and its processing residues
Provedor de dados: 52
Autores: Pessoa,José Dalton Cruz; Arduin,Marcos; Martins,Maria Alice; Carvalho,José Edmar Urano de.
The aim of this work was to study the source of the açaí residue for its possible commercial applications by characterizing the fruit (fresh and dry mass), and performing an anatomic study of the pericarp from which were identified the origin of the anthocyanins, and fatty acids, and fibers; also the vascular system, its fibers constituents and fibrils were characterized. It was concluded that anthocyanins were located on the epiderm and external parenchyma, and that solids retained on the sieve come from the sclerenchyma, and that the fatty acids come from the storage parenchyma. The vascular tissue was formed by the fibers around 20 mm length. The length distribution of the fibrils had a mean length of 580µm.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fiber; Fibril; Composite; Fatty acid; Anthocyanin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600022

Use of image analysis for monitoring the dilution of Physalis peruviana pulp
Provedor de dados: 52
Autores: Licodiedoff,Silvana; Ribani,Rosemary Hoffmann; Camlofski,Ana Mery de Oliveira; Lenzi,Marcelo Kaminski.
The aim of this work was to develop linear models using the image analysis coupled with density measurements to monitor the dilution of the Physalis juice in the concentrations ranging from 0 to 100% in mass of juice pulp. A sample corresponding to 20% in the mass of juice pulp was for validating purposes and a prediction of 19.9±0.3%. The models with three parameters showed the best predictions, providing this technique with a promising future for the monitoring the dilution of fruit juices.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juice; Fruit; Color; Image; RGB; Physalis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300015

Bacteriocinogenic effect of Lactobacillus sakei 2a on microbiological quality of fermented Sardinella brasiliensis
Provedor de dados: 52
Autores: Espírito Santo,Milton L. P.; Beirão,Luiz H.; Sant'Anna,Ernani S.; Dalcin,Eliana Bressa; Franco,Bernadette G. M.
Lactobacillus sakei 2a is a bacteriocin producer strain and, in this work, it's effects as a starter culture in the fermentation process of sardine (Sardinella brasiliensis) fillets were observed at different concentrations of NaCl (2, 4 and 6%) and glucose (2 and 4%), to determine it's ability to produce organic acids and consequent pH reduction. Experiments were carried out independently, with only one parameter (NaCl or glucose) varying at a time. After 21 days of fermentation the deteriorative bacteria concentration reached 9.7 Log10 CFU. g-1 corresponding to 6% NaCl and 4% glucose. Little differences were observed in lactic acid production when 2 and 4% glucose were added, since total acidity was 1.32 and 1.34% respectively, the experiments with 6%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sardinella brasiliensis; Lactobacillus sakei 2a; Fermented fish.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400009

Genome-wide Identiﬁcation, Classiﬁcation and Expression Analysis of the Mildew Resistance Locus O ( MLO ) Gene Family in Sweet Orange ( Citrus sinensis )
Provedor de dados: 52
Autores: Liu,Li-Ping; Qu,Jin-Wang; Yi,Xiao-Qu; Huang,Huan-Huan.
ABSTRACT Mildew resistance Locus O (MLO), a gene family specific to plants, plays significant roles in the resistance to powdery mildew (PM) and response to a variety of abiotic stresses, plant growth and development. Despite their importance as barley, rice, wheat, few studies are reported in dicots except Arabidopsis; no global analysis has been performed in the burgeoning model fruit plant sweet orange (Citrus sinensis). The recent release of the genome sequences of C. sinensis provides an opportunity to conduct a comprehensive overview the evolution and features of the MLO gene family in sweet orange. In this study, amount to 14 members of the Citrus sinensis MLO gene (CisMLO) family according to their gene structures, conserved motifs, and similitude...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; MLO gene family; Powdery mildew; Abiotic stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100426

Generation of intensity duration frequency curves and intensity temporal variability pattern of intense rainfall for Lages/SC
Provedor de dados: 52
Autores: Cardoso,Célio Orli; Bertol,Idelgardis; Soccol,Olívio José; Sampaio,Carlos Augusto de Paiva.
The objective of this work was to analyze the frequency distribution and intensity temporal variability of intense rainfall for Lages/SC from diary pluviograph data. Data on annual series of maximum rainfalls from rain gauges of the CAV-UDESC Weather Station in Lages/SC were used from 2000 to 2009. Gumbel statistic distribution was applied in order to obtain the rainfall height and intensity in the following return periods: 2, 5, 10, 15 and 20 years. Results showed intensity-duration-frequency curves (I-D-F) for those return periods, as well as I-D-F equations: i=2050.Tr0,20.(t+30)-0,89, where i was the intensity, Tr was the rainfall return periods and t was the rainfall duration. For the intensity of temporal variability pattern along of the rainfall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maximum rainfall; I-D-F curves; Gumbel distribution; Intensity rainfall.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200017

New Faunistic Records of Armoured Scale Insects of Southern Iran (Hemiptera: Diaspididae)
Provedor de dados: 52
Autores: Rouhani,Mohammad; Vahedi,Hassan Ali; Marefat,Alireza.
ABSTRACT As a result of recent field work in southern Iran from 2012 to 2016, twenty species of armoured scale insects were collected, representing two subfamilies (Aspidiotinae and Diaspidinae) and 4 tribes including Aspidiotini (8 species in 5 genera), Diaspidini (4 species in 2 genera), Leucaspidini (5 species in 4 genera) and Parlatoriini (3 species in 1 genus). The species Diaspidiotus gigas (Thiem and Gerneck), is illustrated and redescribed from Iran for the first time. In addition, four species are reported and redescribed from south of Iran for the first time: Aspidiotus nerii Bouché, Diaspidiotus armenicus Borchsenius, Leucaspis pusilla Löw, Melanaspis louristana Balachowsky and Kaussari.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diaspididae; Coccomorpha; Tribes; Iran; New record.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100434

Erratum: Anticandidal Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100919

Effects of water deficit and rehydration on the polar lipid and membranes resistance leaves of Phaseolus vulgaris L. cv. Pérola
Provedor de dados: 52
Autores: Martins Júnior,Raimundo Rômulo; Oliveira,Micheline Soares Costa; Baccache,Maria Ary; Paula,Fernando Monteiro de.
Bean leaves (Phaseolus vulgaris L.) cv. Pérola were used to evaluate the water deficit effects in polar lipids composition and in the electric conductivity. The results showed that the water deficiency a effected in the electrolytes loss which increased gradually in response to water deficit. This suggested a compartimentalization loss, due to the structural cellular membranes elements degradation. Total lipids contents decreased by reason of the water stress action. The polyunsaturated fatty acid contents (linoleic and linolenic acids) suffered a decrease while saturated fatty acid (palmitic and stearic acids) increased. The imbalance in the fatty saturated/unsaturated acid relation led to a reduction in the unsaturation index. The electrolytes loss...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polar lipids; Membranes resistance; Water stress.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200016

Frequency of group a rotavirus in piglet stool samples from non-vaccinated Brazilian pig herds
Provedor de dados: 52
Autores: Linares,Rita de Cássia; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Médici,Kerlei Cristina; Feronato,César; Grieder,Wladimir; Alfieri,Amauri Alcindo.
Neonatal diarrhea is one of the main causes of morbidity and mortality in piglets, and it leads to significant economic losses for pig farmers worldwide. The aim of this study was to determine the frequency of diagnosis, age group, and association of group A rotavirus (GARV) infection with diarrhea in piglets from pig herds in two (south and center-west)Brazilian geographical regions. The frequency of GARV diagnosis was evaluated between 2004 and 2007, using SS-PAGE on 681 fecal samples (428 diarrheic and 253 with normal consistency) from 1-4 week-old piglets. The animals were selected from 130 pig herds and 80 counties in the states of Rio Grande do Sul, Santa Catarina, Paraná, Mato Grosso do Sul, and Mato Grosso, Brazil. None of the herds were vaccinated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piglet; Neonatal diarrhea; Group A rotavirus; SS-PAGE.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700009

Analysis of Mercury-Rich plants and mine tailings using the Hydride-Generation AAS method
Provedor de dados: 52
Autores: Moreno,Fábio Netto; Anderson,Christopher; Stewart,Robert; Robinson,Brett.
A hydride-generation atomic absorption spectroscopy (AAS) method was developed for the analysis of total Hg in liquid matrices of mercury-rich plants and mine tailings samples. The detection limit for this method was as low as 11.4 ng/mL. The reproducibility of the mercury signals (in terms of relative standard deviation) was 4.6%. Accuracy of the method was verified by analyses of deionised water samples spiked with HgCl2 and HgNO3. Recovery values for total mercury ranged from 88.5 to 94.3% for both mercury species added. An external certified laboratory validated the analytical method with a maximum discrepancy of 15 %. Optimal analytical response of the equipment for Hg analysis of plant samples was achieved when the sample volume added to the reaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydride-generation; Mercury; Plant and mine tailings; Sodium borohydride (NaBH4); Phytoextraction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400019

Three tubulin genes of Trichoderma harzianum: alpha, beta and gamma
Provedor de dados: 52
Autores: Li,Min; Yang,Qian; Song,Jinzhu.
Three tubulin genes of Trichoderma harzianum were cloned followed genomic walking procedure. The tubulins showed high degree of amino acid homology with other fungal tubulins and were homologous with each other with 32 to 38% amino acid identity. Three measures for the degree of codon usage bias indicated the presence of bias in all the sequences, suggesting high expression levels in all the genes. Protein structures were modeled to provide the basis for understanding the tubulin's properties and its interactions with microtubule-associated proteins. Potential motifs were also postulated.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma harzianum; Tubulin; Codon usage bias; Motif.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400009

Diversity of the Potential 2-Methylisoborneol-Producing Genotypes in Thai Strains of Planktothricoides (Cyanobacteria)
Provedor de dados: 52
Autores: Tawong,Wittaya.
ABSTRACT The genus Planktothricoides Suda &amp; Watanabe is considered as a 2-methylisoborneol (MIB) producer, affecting water quality and aquatic animal products worldwide. To date, there is limited information about the diversity of this genus from Thailand. In this study, Thai Planktothricoides strains were isolated from fish ponds and reservoirs in North, Northeast and Central regions for morphological examination, phylogenetic analyses based on 16S rRNA, rbcLX and MIB synthase genes as well as GC/MS/MS analyses. The morphological results and the 16S rRNA and rbcLX phylogenies of Thai Planktothricodes strains enabled them to be designated as Planktothricoides raciborskii. Cell dimensions of Thai strains tested were in 1.86 to 5.96 µm length (L), 2.83...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-methylisoborneol; Filamentous cyanobacteria; 16S rRNA; MIB synthase; Thailand.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100433

Spectrophotometric determination of Pd(Ii) with P-Dimethylaminebenzylidenerhodanine
Provedor de dados: 52
Autores: Dias,Lucia Felicidade; Nozaki,Jorge.
p-Dimethylaminebenzylidenerhodanine in methyl isobutyl ketone was employed as selective and sensitive reagent for the extraction and determination of palladium(II) in metal alloys and rocks. Beer’s Law is followed in the concentration range 0.2-2.4 m g.mL-1 of organic phase with a molar absorptivity of 3.0x10(4)L.mol-1.cm-1. The detection limit observed for aqueous solution was 0.1 mg.L-1. The extractions were performed at pH 2.4 and no interference of foreign ions such as Cu2+, Zn2+, Fe3+, Pb2+, Hg2+ ,etc., were observed in jewellery alloy and rock sample analyzed. The complex of reagent and palladium(II) was pH dependent and it should be carefully controlled during extraction. Continuous variation and mole ratio methods showed that the complex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-dimethylaminebenzylidenerhodanine; Palladium(II); Extraction-spectrophotometry; Rocks; Metal alloy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200003

Formulation and evaluation of glipizide floating-bioadhesive tablets
Provedor de dados: 52
Autores: Patel,Jayvadan K.; Chavda,Jayant R.
The purpose of this study was formulation and in vitro evaluation of floating-bioadhesive tablets to lengthen the stay of glipizide in its absorption area. Effervescent tablets were made using chitosan (CH), hydroxypropyl methylcellulose (HPMC), carbopolP934 (CP), polymethacrylic acid (PMA), citric acid, and sodium bicarbonate. Tablets with 5% effervescent base had longer lag time than 10%. The type of polymer had no significant effect on the floating lag time. All tablets floated atop the medium for 23-24 hr. Increasing carbopolP934 caused higher bioadhesion than chitosan (p &lt; 0.05). All formulations showed a Higuchi, non-Fickian release mechanism. Tablets with 10% effervescent base, 80% CH/20% HPMC, or 80% CP/20% PMA seemed desirable.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glipizide; Floating-bioadhesive; Tablets; Non-Fickian release.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500010

Characterization of Alkaliphilic Bacteria Isolated from Bauxite Residue in the Southern Region of Minas Gerais, Brazil
Provedor de dados: 52
Autores: Nogueira,Elis Watanable; Hayash,Elize Ayumi; Alves,Enne; Lima,Claudio Antônio de Andrade; Adorno,Maria Talarico; Brucha,Gunther.
ABSTRACT The aim of this study was to isolate and characterize bacterial strains from bauxite residue in the southern region of Minas Gerais, Brazil, by 16S rRNA gene sequencing and biochemical assays. Bacillus cohnii, Bacillus pseudofirmus, and Bacillus clarkii were identified among the isolates. The isolates were able to use a wide range of carbon sources and to grow at NaCl concentrations of up to 10%, temperatures from 10 to 40 °C, and pH from 7 to 10.5, producing a wide variety of organic acids. This is the first report on microbial composition of bauxite residue in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauxite residue; 16S ribosomal RNA gene; Alkaliphilic bacteria.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100406

Identification of a splicing coactivator gene that affects the production of ochratoxin a in Aspergillus carbonarius
Provedor de dados: 52
Autores: Lunardi,Lígia Uno; Guembarovski,Roberta Losi; Hanai,Luiz Ricardo; Cristiano,Valderi; Vieira,Maria Lucia Carneiro; Sartori,Daniele; Fungaro,Maria Helena Pelegrinelli.
Ochratoxin A is a mycotoxin produced by some fungi species. Among them, Aspergillus carbonarius is considered a powerful producer. Genes involved in the ochratoxin A biosynthesis pathway have been identified in some producer species. However, there are few studies that purpose to identify these genes in A. carbonarius. The use of insertion mutants to identify genes associated with certain properties has been increased in the literature. In this work, the region of T-DNA integration was investigated in one A. carbonarius ochratoxin-defective mutant previously obtained by Agrobacterium tumefaciens-mediated transformation, in order to find an association between interrupted gene and the biosynthesis of ochratoxin A. The integration occurred in a gene that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxigenic fungi; Mycotoxin; Real time PCR; Splicing coactivator protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700018

Performance of maize landrace under no-till as affected by the organic and mineral fertilizers
Provedor de dados: 52
Autores: Caires,Eduardo Fávero; Joris,Helio Antonio Wood; Churka,Susana; Zardo Filho,Renato.
The aim of this work was to study the effects of organic and mineral fertilizers at sowing (without fertilizers, organic poultry litter fertilizer on the surface and mineral NK + reactive natural phosphate from Arad and NK + triple superphosphate in the furrow) and topdressing (without fertilizers, organic poultry litter fertilization and urea) on chemical attributes of a no-till Oxisol and nutrition and yield of maize landrace (Zea mays L.), Carioca variety in a field experiment. Results revealed that P content (Mehlich 1 and resin) was increased in the soil surface layer with organic poultry litter fertilizer on the surface at sowing. Mineral fertilizer in the sowing furrow could be replaced by organic fertilizer with poultry litter on the surface, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Reactive natural phosphate; Nitrogen fertilization; Poultry litter.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200006

Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation
Provedor de dados: 52
Autores: Luna,Christine Lamenha; Lopes,Carlos Edison; Massarani,Giulio.
The aim of this work was use flocculation/sedimentation and flotation for recovery of spores of the Bacillus sphaericus. Microorganism was produced batchwise using culture medium based skimmed milk, corn steep liquor and mineral salts. The best results of flocculation were obtained using CaCl2.2H2O, FeCl3.6H2O, Al2(SO4)3 and tannin as flocculating agents, with optimal flocculation concentrations of 1,500, 3,000, 2,000 and 1,700ppm, respectively. Flocculent suspensions were characterized based on floc diameter and density. Settling tests were performed in batch at different concentrations of the cellular suspensions and revealed high recovery of the solids in suspension in all cases. Flotation tests were accomplished using a mechanical agitated flotation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus sphaericus; Flocculation; Sedimentation; Flotation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400008

Carbohydrate/glycan-binding specificity of legume lectins in respect to their proposed biological functions
Provedor de dados: 52
Autores: Ramos,Márcio Viana; Grangeiro,Thalles Barbosa; Cavada,Benildo Sousa; Shepherd,Iain; Lopes,Roberval Oliveira de Melo; Sampaio,Alexandre Holanda.
The lectins, proteins which specifically recognize carbohydrate moieties, have been extensively studied in many biochemical and structural aspects in order to establish the molecular basis of this non-catalytic event. On the other hand, their clinical and agricultural potentials have been growing fast. Although lectins, mainly those from legume plants, had been investigated for biological properties, studies about the physiological functions of lectins are scarce in literature. Therefore, despite the accumulated data on lectins (as proteins), the role played by these signalizing molecules is poorly discussed. In the light of our accumulated results on legume lectins, specially those obtained from plants belonging to the Diocleinae sub-tribe and available...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Binding-site; Biological functions; Fine sugar-specificity; Legume lectins.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400001

Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae)
Provedor de dados: 52
Autores: Mendes-Bonato,Andréa Beatriz; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
Microsporogenesis was analyzed in an interspecific hybrid between an artificially tetraploidized sexual accession of Brachiaria ruziziensis (2n=4x=36) and a natural apomictic tetraploid accession of B. decumbens. Syncytes involving a large number of cells were recorded in 15.4% of meiocytes. Meiosis progressed normally in syncytes during prophase I; in metaphase I, however, several nuclei were found fusioned, showing chromosome stickiness and several chromosome fragments. Meiosis was arrested in metaphase I and pycnotic nuclei and micronuclei were formed. Abnormal cytokinesis fractionated the syncyte into abnormal meiotic products that were covered by the pollen wall. Meiocytes in leptotene were recorded in all the slides prepared for both meiotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria decumbens; Brachiaria ruziziensis; Cell fusion; Interspecific hybrid; Meiosis arrest; Pollen sterilitiy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500011

Modeling the Growth of Byssochlamys fulva on Solidified Apple Juice at Different Temperatures
Provedor de dados: 52
Autores: Longhi,Daniel Angelo; Tremarin,Andréia; Carciofi,Bruno Augusto Mattar; Laurindo,João Borges; Aragão,Gláucia Maria Falcão de.
The aim of this study was to establish primary and secondary models to describe the growth kinetics of Byssochlamys fulva on solidified apple juice at different temperatures. B. fulva was inoculated on solidified apple juice at 10, 15, 20, 25 and 30 °C. Linear-with-breakpoint, Baranyi and Roberts, and Huang primary models (without upper asymptote) were fitted to the data, and they showed good ability to describe the growth kinetics. B. fulva showed longer adaptation time on apple juice than on culture medium, but growth rates were similar as reported in the literature. The dependence of µmax and λ parameters on temperature was described with Square Root and Arrhenius-Davey secondary models, respectively. These models were important to establish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mould growth; Mathematical modeling; Food microbiology; Predictive mycology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600971

Offshore distribution patterns of the cyanobacterium Trichodesmium erythraeum ehrenberg and associated phyto- and bacterioplankton in the southern Atlantic coast (Paraná, Brazil)
Provedor de dados: 52
Autores: Siqueira,Adriana; Kolm,Hedda Elisabeth; Brandini,Frederico Pereira.
Studies were carried out on Thrichodesmium erythraeum occurring on the inner shelf in the state of Paraná, Brazil. Temperature, salinity, rainfall, wind velocity, total bacteria, bacterial biomass, chlorophyll-a, phytoplankton, Anabaena sp., Merismopedia sp. and T. erythraeum densities were measured in surface water. Centric and pennate diatoms, Anabaena sp. and Merismopedia sp. were most abundant at 15 m isobath, while dinoflagellate abundance was relatively constant among stations. Similarly, total bacterial densities were relatively homogeneous throughout the sampling area, suggesting that blooms of T. erythraeum were not yet in the senescent phase. Results showed that T. erythraeum was capable of surviving in relatively inhospitable environmental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichodesmium erythraeum; Bloom; Cyanobacteria; Phytoplankton; South Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300018

Assessment of the DNA Damage in Human Sperm and Lymphocytes Exposed to the Carcinogen Food Contaminant Furan with Comet Assay
Provedor de dados: 52
Autores: Pandir,Dilek.
ABSTRACTThe aim of this work was to assess the damage of DNA in human blood cell and spermin vitro under the influence of furan. These cells were administered 0-600 μM of furan at 37 and 32oC for 30 and 60 min, respectively. A significant increase in tail DNA%, tail length and moment indicating DNA damage was observed at increasing doses when compared to the controls. The treatment with 300 and 600 μM of furan showed a maximum increase of 86.74 ± 2.43 and 93.29 ± 8.68 compared to the control tail DNA% in lymphocytes. However, only 600 μM of furan showed a maximum increase of 94.71 ± 6.24 compared to the control tail DNA% in sperm. The results suggested that furan caused DNA damage in lymphocytes at increasing doses, but appeared to have not the same effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Furan; Tail length; DNA damage; Human blood cells; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500773

Radiolabeling and biodistribution of monoclonal antibody (MAb) anti-CD20 with iodine-131
Provedor de dados: 52
Autores: Akanji,Akinkunmi Ganiyu; Muramoto,Emiko; Caldeira Filho,José de Souza; Couto,Renata Martinussi; Araújo,Elaine Bortoletti de.
Radioactive isotopes of iodine, mainly iodine-131 have been broadly used in many monoclonal antibody radioiodination settings, employing different methods. In this study, using a Chloramine-T procedure, the influence of incubation time, CT mass, and Ab/activity ratio on the radiochemical yield of the anti-CD20 antibody labeled with iodine-131 was observed. Radiochemical yield was &gt; 97 %, employing different Chloramine-T masses, independently of incubation time. Radiochemical purity was above 99 % after purification of the labeled compound. The relationship between Ab mass and radioactivity was tested and no difference was observed when 90.6 MBq (2.45mCi) of activity was incorporated in the Ab-mass range studied. Biodistribution study in normal Swiss...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoclonal antibody; Radioioactivity; Anti-CD20; Radioimmunotherapy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700010

Seasonal influence on the essential oil production of Nectandra megapotamica (Spreng.) Mez
Provedor de dados: 52
Autores: Amaral,Lúcio de Paula; Tondolo,Juliana Simoni Moraes; Schindler,Bianca; Silva,Daniela Thomas da; Pinheiro,Carlos Garrido; Longhi,Solon Jonas; Mallmann,Carlos Augusto; Heinzmann,Berta Maria.
This study evaluated the seasonal influence on the yield and chemical composition of the essential oil (EO) of Nectandra megapotamica. Fresh young (YL) and old leaves (OL) obtained from three trees in each season (Nov/2010 to Sep/2011) collected in Santa Maria-RS were hydrodistilled in triplicate. The chemical composition was determined by the gas chromatography coupled to mass spectrometry (GC-MS) and the yield on dry basis was evaluated by two-way ANOVA (seasons, development stage). Spring (Sp) and summer (Su) showed higher average incomes (0.45 and 0.33%), which occurred when flowering, fruiting, and growth of YL and senescence of OL took place, while autumn (Au) presented the lowest yield (0.25%) during the rustification of OL. The highest yield was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lauraceae; Phenology; Variability of volatile oils; Terpenes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100012

Fruit development, pigmentation and biochemical properties of wax apple as affected by localized Application of GA3 under field conditions
Provedor de dados: 52
Autores: Khandaker,Mohammad Moneruzzaman; Boyce,Amru Nasrulhaq; Osman,Normaniza; Golam,Faruq; Rahman,M. Motior; Sofian-Azirun,M.
This study investigated the effects of gibberellin (GA3) on the fruit development, pigmentation and biochemical properties of wax apple. The wax apple trees were rubbing treated with 0, 20, 50 and 100 mgGA3/l under field conditions. The localized application (rubbing) of 50 mg GA3/l significantly increased the fruit set, fruit length and diameter, color development, weight and yieldcompared to the control. In addition, GA3 treatments significantly reduced the fruit drop. With regard to the fruit quality, 50 mg/l GA3 treatment increased the juice content, K+, TSS, total sugar and sugar acid ratio of wax apple fruits. In addition, higher vitamin C, phenol, flavonoid, anthocyanin, carotene content, PAL and antioxidant activities were recorded in the treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fruit development; Pigmentation; Quality; GA3; Wax apple.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100002

Bionomic aspects of Pattonella intermutans (Thomson, 1869) (Diptera, Sarcophagidae) under laboratory conditions
Provedor de dados: 52
Autores: Oliveira,Vanderleia Cristina de; Mello,Rubens Pinto; Santos,Rosicleide Fátima Silva.
Life and fertility tables of Pattonella intermutans (Thomson, 1869) were studied in the laboratory conditions. The flies colonies were maintained at 20-28°C, 80±10% RH and observed daily. Life expectancy was 2.34 weeks for males and 2.33 weeks for females. The survivorship curves presented a type II tendency for females and type III for males. The periods of pre-larviposition and larviposition were of 2.5 and 9.5 weeks, respectively. Using data of the life table of fertility, the following values were obtained: Ro=24.83 times; T= 5.06 weeks; r=0.64 and lambda=1.89. The number of generations of P. intermutans was estimated to be 10.28 generations/year. The females had a high fertility under laboratory contiditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diptera; Sarcophagidae; Pattonella intermutans; Bionomic; Ecology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600011

Influence of mulberry leaf extract on serum adiponectin, visfatin and lipid profile levels in type 2 diabetic rats
Provedor de dados: 52
Autores: Tond,Sepideh Barzin; Fallah,Soudabeh; Salemi,Zahra; Seifi,Morteza.
ABSTRACT The effect of ethanolic mulberry leaf extract (MLE) and mulberry leaf powder (MLP) on glycemic control, serum adiponectin, visfatin and lipid profile in type2 diabetic rats have been investigated. 30 male wistar rats randomly divided into 5 groups. One group was randomly assigned as control (I) and diabetes was induced in others by administration of streptozotocin (STZ) (55 mg/kg body weight) 15 minutes after the administration of nicotinamide (110 mg/kg body weight) intraperitoneally. Finally, fasting blood glucose (FBG), lipid profile, adiponectin and visfatin were assessed after 6 weeks. Lipid profiles including serum FBG, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-c), very low-density lipoprotein cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mulberry leaf extract; Mulberry leaf powder; Type2 diabetes; Adiponectin; Visfatin.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100336

Regulating Effect Of Carnosine And /Or L- Arginine On The Expression Of Inflammatory Molecules Induced Nephropathy In The Hypoxic Rat Model
Provedor de dados: 52
Autores: Al-Rasheed,Nouf M.; Fadda,Laila; Mohamed,Azza M; Attia,Hala A.; Al-Rasheed,Nawal M.
This study aimed to explore the effective role of carnosine and /or L- arginine in down regulation of the inflammatory molecule expression caused renal damage in response to sodium nitrite (NaNO2) induced hypoxia in rats . NaNO2 was administered subcutaneously (s.c.) to rats as a single dose (60 mg/kg body weight ). L-arginine (200mg/Kg body weight) and carnosine (250 mg/ Kg body weight ) were administered (i.p.) as a single dose , 24 h before NaNO2 injection. The results revealed that pre- administration of arginine and /or carnosine to NaNO2 hypoxic rats, significantly modulated the increases in serum markers of renal function (creatinine and urea) as well as the decrease in hemoglobin (Hb) level versus hypoxic rats. The two agents each alone or in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoxia; Arginine; Carnosine; Inflammatory molecules; Renal damage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100310

Erratum: Assessment of Sugarcane Germplasm (Saccharum spp. complex) Against Red Rot Pathogen Colletotrichum Falcatum
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100917

Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption
Provedor de dados: 52
Autores: Sugahara,Vanessa Hitomi; Varéa,Geni da Silva.
Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Lipase; Beauveria bassiana; Silica gel.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600842

Analysis of seven STR human loci for paternity testing by microchip electrophoresis
Provedor de dados: 52
Autores: Fraige,Karina; Travensolo,Regiane Fátima; Carrilho,Emanuel.
The aim of this work was to evaluate two paternity cases by microchip electrophoresis and the validation of the methodology by comparison of the results with those obtained in a commercial genetic analyzer. It was observed that when working with tetranucleotide regions, in which the minimal difference between the alleles was only four base pairs, the commercial microchip system did not present the resolution and repeatability needed. Nevertheless, the relative standard deviation was between 0 and 1.2% and the fragments detected were within the expected size ranges as described in the literature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioanalyzer 2100; Paternity testing; Polymerase chain reaction; Short tandem repeats.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200006

Influence of the reactor shape on the kinetics of ethanol production in laboratory-scale batch fermentation tests carried out in unstirred vessels
Provedor de dados: 52
Autores: Borzani,Walter; Souza,Fabiana Saraguza de; Soyama,Claudia Yoshie; Furuko,Patricia Marie; Ferreira,Daniela Souza.
The influence of the shape of laboratory-scale unstirred reactors on the kinetics of ethanol production in batch ethanol fermentation was studied. Two reactors were used: a 1-L glass measuring cylinder and a 2-L Erlenmeyer flask. The volume of inoculated medium in each reactor was 1,000 mL. The above influence was affected by the ratio between the initial yeast cells concentration (X0: ~ 7 g/L, ~ 14 g/L, and ~ 21 g/L, dry matter) and the initial glucose concentration (S0: ~ 100 g/L, ~ 150 g/L and ~ 200 g/L). When X0/S0 increased from 0.038 to 0.219 the influence of the reactor shape decreased, and was not observed when X0/S0 = 0.22 to 0.24. The reactor shape practically did not affect the ethanol yield, the final yeast cells concentration and both the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Batch ethanol fermentation; Ethanol production kinetics; Reactor shape; Unstirred reactor.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400019

Volatile compounds present in traditional meat products (charqui and longaniza sausage) in Chile
Provedor de dados: 52
Autores: Gianelli,María Pía; Salazar,Vanessa; Mojica,Luis; Friz,Miguel.
The aim of this work was to identify and quantify the volatile compounds in five different commercial brands of charqui and longaniza sausages. Volatile compounds were extracted from some samples headspace using solid phase microextraction (SPME). The identification and quantification were made through the gas chromatography with a mass-selective detector (GS-MS). Fifty-four volatile compounds were identified in charqui samples and thirty-two volatile compounds in longaniza sausages. The chemical groups of the volatile compounds found in both the products were: aldehydes, alcohols, ketones, organic acids, furans, aromatic and aliphatic hydrocarbons. Significant differences were found (p&lt;0.05) in the volatile compounds among the brands of longaniza and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SPME; Volatile compounds; Sausage; Charqui.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400017

Photovoltaic Cell Temperature Estimation for a Grid-Connect Photovoltaic Systems in Curitiba
Provedor de dados: 52
Autores: Yang,Renata Lautert; Tiepolo,Gerson Máximo; Tonolo,Édwin Augusto; Urbanetz Junior,Jair; Souza,Muriele Bester de.
Abstract The photovoltaic solar energy has been growing in installed capacity worldwide year by year, and Brazil has also been investing in this renewable source of energy generation. The conversion of light into electrical energy occurs in the photovoltaic cells, which are sensitive to the increase of the temperature. A considerable amount of the energy incident on the module is transformed into heat, rising its temperature and decreasing its efficiency. This study aims to estimate the temperature in photovoltaic cells (Tc) for polycrystalline silicon modules from a grid-connected photovoltaic systems through several equations proposed over the last decades by researchers. This estimation will occur in the municipality of Curitiba, using data from INMET’s...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic solar energy; Grid-connected photovoltaic system; Estimation of the photovoltaic cell temperature.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200215

Impact of the construction of the harbor at pecém (Ceará, Brazil) upon reef fish communities in tide pools
Provedor de dados: 52
Autores: Freitas,Marcelo Carneiro de; Vieira,Regine Helena Silva dos Fernandes; Araújo,Maria Elisabeth de.
This study aimed to assess the impact of harbor construction at Pecém (Brazil) upon the reef fish communities in tide pools. The monitoring was carried out through a visual census and sea water quality was evaluated by microbiological analyses. From March to December 2001, 1,425 individuals of 17 families and 25 species were registered. The most frequent species were Haemulon parra, Acanthurus chirurgus, Abudefduf saxatilis and Sparisoma axillare. The average species diversity for the whole period was 1.77 bits.ind -1. There was evidence that the tide pools gradually silted up, resulting in a reduction in species diversity and richness. The microbiological analyses indicated the presence of faecal coliforms in the water though not at significant levels....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine fishes; Environmental changes; Sand dredging.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100024

Effect of microhabitat distribution and substrate roughness on barnacle Tetraclita stalactifera (Lamarck, 1818) settlement
Provedor de dados: 52
Autores: Skinner,Luís Felipe; Coutinho,Ricardo.
Studies were carried out on microhabitat distribution and substrate roughness on barnacle settlement at Cabo Frio region, Rio de Janeiro, Brazil. Ten commercial blocks of granite rock (100 cm²) were attached to rocky coast at 0.6 tide level (5 smooth blocks and 5 rough with 20 holes). Experiment was conduced for five weeks, with repetitions each week. Settlement was higher on rough (398 individuals) than on smooth blocks (38 individuals) on the same week. There was no significant difference between settlement in the centre of the block and at the edge. Significant differences observed among weeks were affected by the variability of cyprid larvae density. This result showed that roughness was an important variable to be considered on barnacle settlement.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barnacle; Substrate roughness; Settlement; Upwelling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100014

Pseudomonas Aeruginosa: Virulence Factors and Antibiotic Resistance Genes
Provedor de dados: 52
Autores: Rocha,Antônio José; Barsottini,Mario Ramos de Oliveira; Rocha,Renan Rhonalty; Laurindo,Maria Vitória; Moraes,Francisco Leandro Laurindo de; Rocha,Soraya Lília da.
Abstract In this review, we explore some aspects of Pseudomonas aeruginosa virulence factors that are related to disease development in healthy organisms and resistance to antibiotics. This pathogen is one of the most clinically and epidemiologically important bacteria in Brazil, being the major cause of opportunistic infections. Among the virulence factors, biofilm formation acting of manner different in the organism. Furthermore, we review several P. aeruginosa genes that act in antimicrobial resistance, such as β-lactamases against β-lactamers. The resistance to pied-lactamases in P. aeruginosa is associated to resistance to the broad-spectrum cephalosporin. On the other hand, there is a group of synthetic broad-spectrum antibiotics acting on DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Virulence factors; Antimicrobial activities; Biofilms; Β-lactamase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100902

Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding
Provedor de dados: 52
Autores: Abyari,Mohammad; Nasr,Nasrin; Soorni,Jahad; Sadhu,Deokule.
In this study, the various concentrations of casein hydrolysate (25, 50, 75, 100 mg/L) and L-phenylalanine (50, 100, 150, 200 µM/l) were incorporated in MS containing 15 µM BA plus 5 µM 2,4-D for enhancement of secondary metabolites in cell culture of Spilanthes acmella. The presence of casein hydrolysate in the nutrient medium improved the growth of cell biomass and the production of scopoletin. The addition of casein hydrolysate up to 75 mg/L stimulated the accumulation of scopoletin, but increasing excess 75 mg/L the level of casein hydrolysate reduced the production of scopoletin. The addition of L-phenylalanine in the nutrient medium was found to be more effective for production of secondary metabolite in S. acmella. The addition of 50 µM/L of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Casein hydrolysate; L-phenylalanine; Precursor; Scopoletin; Spilanthes acmella.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100410

Diel Variations and Ecological Aspects in Fish Assemblages of a Sandy Beach in the Semi-Arid Region of Northeast Brazil.
Provedor de dados: 52
Autores: Dantas,Natália Carla Fernandes Medeiros; Silva Júnior,Carlos Antônio Beserra; Lippi,Daniel Lino; Feitosa,Caroline Vieira.
ABSTRACT For the demersal fish that inhabit sandy beaches, the variation between day and night periods represents a determinant factor in their behavior, especially for species that live in shallow waters. This research aims at determining whether there are differences in the fish assemblage structure of the sandy beach of São Cristóvão, RN, Brazil, between diurnal and nocturnal periods. Samplings were carried out in 2011, during spring high tide period, with an otter trawl net. Dissimilarity between diurnal and nocturnal fish assemblages was confirmed by PERMANOVA results. No statistical differences were observed for richness and diversity of fish assemblage between day and night periods. The first and third order carnivores were the significantly more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Demersal ichthyofauna; Density; Diversity; Total length; Day; Night.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100347

Interannual variation of larval fish assemblages in the Gulf of Cádiz (SW Iberian Peninsula) in relation to summer oceanographic conditions
Provedor de dados: 52
Autores: Mafalda Júnior,Paulo; Rubín,Juan Perez de.
Two ichthyoplankton surveys were conducted during July 1994 and July 1995 in the Gulf of Cádiz with the aim of describing composition, abundance, distribution patterns and interannual variations of larval fish assemblages. Interannual differences were found in this study. In 1994, higher salinities were observed at external sites, though in 1995, higher values were observed at intermediate sites. The upper water column was warmer in 1994 and had less fish larvae density. During 1994, Sardinella aurita and Engraulis encrasicolus were abundant but spatial location was opposite. In 1995, abundance of both species was very different, but with similar spatial pattern. Cluster analysis revealed well-defined groups of stations and assemblages of larvae, primarily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Larval fish assemblages; Sardinella aurita; Engraulis encrasicolus; Gulf of Cádiz.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300014

Diallel analysis of the combining ability of common bean (Phaseolus vulgaris L.) cultivars
Provedor de dados: 52
Autores: Barelli,Marco Antônio Aparecido; Gonçalves-Vidigal,Maria Celeste; Amaral Júnior,Antonio Teixeira do; Vidigal Filho,Pedro Soares; Scapim,Carlos Alberto.
Six common bean cultivars (LPSPI 93-17, LPSPI 93-19, FT-Nobre, Aporé, Rudá and Campeão-1) and their 15 diallel hybrids were evaluated for four agronomic characteristics using Griffing's methodology (1956). The analysis indicated the predominance of non-additive gene effects for days to germination and plant height. The mean number of days to flowering and mean height of the first pod insertion were predominantly controlled by genes showing additive effects. The results showed that LPSI 93-19 and Aporé were indicated for intra-population breeding, while the combinations LPSPI 93-17 x FT-Nobre, LPSPI 93-17 x Aporé, LPSPI 93-17 x Rudá, LPSPI 93-19 x Campeão-1, FT-Nobre x Aporé and Rudá x Campeão-1 were indicated for interpopulational breeding. Plants with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Common bean; Phaseolus vulgaris; Diallel analysis; Plant breeding.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400009

Concentration of radioactive elements (U, Th and K) derived from phosphatic fertilizers in cultivated soils
Provedor de dados: 52
Autores: Becegato,Valter Antonio; Ferreira,Francisco José Fonseca; Machado,William César Pollonio.
Gamma spectrometric measurements were obtained for the agricultural soils aiming at characterizing the spatial distribution of radionuclide concentrations (K, eU and eTh), as well for the samples of phosphatic fertilizers and agricultural gypsum. In the study areas, three types of soils occured: Eutrophic Red Nitosol (Alfisoil), Eutroferric Red Latosol of clayey texture (Oxisoil) and Dystrophic Red Latosol of medium texture (Oxisoil). The results showed that the radionuclide concentrations in more clayey soils were higher than in more sandy soils, mainly as a function of a higher adsorption capacity of the former. For the area where human activity predominated, the average contents of K, eU and eTh were respectively 54.75; 10.22 and 7.27 Bq/Kg,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma-rays spectrometry; Soils; Fertilizers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600022

Calculation of uncertainties in influence quantities in biological essays
Provedor de dados: 52
Autores: Morel,Pierre; Arruda,Tatiana Langbeck de; Bohrer-Morel,Maria Beatriz C.
Due to the need of showing the reliability of results from biological essays, such as in health and environmental areas, laboratories have been implementing quality systems, particularly ABNT NBR ISO/IEC 17025 (ABNT, 2001), which include as one of the requirements, the calculation of uncertainty in essays results. It is of common knowledge that estimating uncertainty in essays using organisms, such as animal and vegetal species, is difficult, preventing from meeting that requirement in the standard. The paper shows the calculation of uncertainties in influences quantities in these essays as a tool to allow validating the measuring process, by using the essays performed by Rhodia Environmental Impact Analysis Laboratory and IPEN Ecotoxicology Laboratory as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uncertainty; Influence quantities; Biological essays.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200015

Glucose metabolism in rats submitted to skeletal muscle denervation
Provedor de dados: 52
Autores: Nunes,Wilton Marlindo Santana; Mello,Maria Alice Rostom de.
This study analyzed the local and systemic effects of immobilization by denervation of the skeletal muscle on glucose metabolism. The rats were submitted to section of the right paw sciatic nerve. A reduction was observed in glucose uptake by the isolated soleus muscle of the denervated paw after 3 and 7 days, but not after 28 days in relation to the control animals. There was no difference after 3 and 7 days in glucose uptake by the soleus muscle of the opposite intact paw in relation to the control. There was increased glucose uptake in the same paw 28 days after denervation. The rate of glucose removal in response to exogenous insulin after 28 days of denervation was significantly higher than in control animals and those observed after 3 and 7 days of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Denervation; Glucose metabolism; Skeletal muscle.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500006

Developmental Toxicity of (4S)-2- (4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic acid in Zebrafish ( Danio rerio )
Provedor de dados: 52
Autores: Akbulut,Cansu; Öztürk,Burcu; Genc,Hayriye; Zengin,Mustafa; Yön,Nazan Deniz.
ABSTRACT (4S)-2-(4-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid is new synthesized substance obtained from cysteine and valine. Thiazolidine derivates have important biological responses so scientists work intensively on these compounds in recent years. It is obvious that thiazolidine contained compounds will be used in future in the pharmaceutical industry to treat important diseases. Median lethal concentrations (LC50) for 48 h and 96 h were found as 1.106±0.052 mM and 0.804mM ± 0.102 respectively. According to LC50, exposure doses were determined as control, 0.4 mM, 0.2 mM and 0.1 mM (4S)-2-(4-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid. Developmental toxicity and apoptotic features on zebrafish development were evaluated in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thiazolidine; Toxicity; Development; Zebrafish; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100431

The influence of caffeine and thymol on the survival, growth and reproduction of Subulina octona (Brugüière, 1789) (Mollusca, Subulinidae)
Provedor de dados: 52
Autores: Ferreira,Paula; Soares,Geraldo Luiz Gonçalves; D'ávila,Sthefane; Bessa,Elisabeth Cristina de Almeida.
Subulina octona is a terrestrial snail which serves as an intermediate host for the parasites. It is also an agricultural pest. The aim of this work was to assess, during 120 days, the effects of caffeine and thymol at 2.5 g/L and 5 g/L on the hatchability, survival after hatching, growth and reproduction of S. octona under the laboratory conditions. A total of 240 eggs, 240 juveniles aged 10-day-old, and 240 aged 30-day-old were tested. The results showed that thymol (at 2.5 g/L and 5 g/L) and caffeine (at 5 g/L) acted as ovicides. In the 10-day-old juveniles, caffeine at 5 g/L caused 25% mortality and at 2.5 g/L it caused 30% mortality. Thymol at 2.5 and 5 g/L caused 20 and 22.5% mortality, respectively. In the 30-day-old juveniles, caffeine at 5 g/L...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeine; Thymol; Molluscicide; Subulina octona; Snail-terrestrial.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400018

Age-dependent fecundity of Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) with sublethal doses of gammacyhalothrin
Provedor de dados: 52
Autores: Pereira,Alexandre Igor Azevedo; Ramalho,Francisco de Sousa; Bandeira,Catarina de Medeiros; Malaquias,José Bruno; Zanuncio,José Cola.
Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) was exposed to gammacyhalothrin to study the stimulatory effect of low concentrations of this pyrethroid. The doses studied were 23.4375, 11.7188, 5.8594, 2.9297, 1.4648, 0.7324 mg.litre-1 and water, as control. The third, fourth, and fifth instars of P. nigrispinus were shorter with all the doses of this insecticide. The survival and longevity of this predator was highest with the lowest doses. The oviposition period of P. nigrispinus decreased as the doses increased. The lowest pre-and postoviposition periods were found with 11.7188 mg.litre-1 of gammacyhalothrin. The dose 0.7324 mg.litre-1 increased the clutch interval. The egg viability was similar between the treatments. The number of eggs per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predator; Pyrethroid; Stink bug; Hormesis; Biology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500013

Morphology of the pericarp and seed of Tabebuia chrysotricha (Mart. ex DC.) Standl. (Bignoniaceae)
Provedor de dados: 52
Autores: Souza,Luiz Antonio de; Iwazaki,Maisa C.; Moscheta,Ismar S.
The morphology and anatomy of the fruit and seed of Tabebuia chrysotricha (Mart. ex DC.) Standl. (Bignoniaceae), a species typical of the Atlantic rainforest, is presented and discussed. The fruit was a loculicidal capsule, dehiscing through two slits, liberating a seminiferous column. The dehiscence process of the fruit involved separation tissue and two crossed sclerenchymatous mesocarpic layers (middle and subepidermic). The seed originated from an anatropous, unitegmic and tenuinucellate ovule. It was exotestal and presented polyembriony. The endothelium and endosperm were conserved in the mature seed and protected the embryo. Adventive embryos were made up of hypostase cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tabebuia chrysotricha; Bignoniaceae; Fruit; Seed; Anatomy; Morphology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300012

Thymbra Spicata Var. Intricata Induces Mesenchymal Stem Cell Proliferation and Osteogenic Differentiation
Provedor de dados: 52
Autores: Mendi,Ayseqül; Yağcı,Beyza Gökçınar; Kızıloğlu,Mustafa; Saraç,Nurdan; Uğur,Aysel; Yılmaz,Derviş; Uçkan,Duygu.
ABSTRACT A natural agent that maintains mesenchymal stem cell (MSCs) viability, promotes osteogenic differentiation while modulating the immunological response could achieve success in regeneration during healing and may also prevent bone resorption and improve regeneration. We aimed to demonstrate that a Thymbra spicata var. intricata extract could induce proliferation, differentiation, and modulate the immune responses of mesenchymal stem cells (MSCs). Using xCELLigence, a real-time monitoring system, we obtained a growth curve of MSCs. A dose of 10 µg/mL was the most efficient concentration for vitality. Osteogenic differentiation and antiinflammatory activities were determined by using an ELISA Kit to detect early and late markers of differentiation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cell; Osteogenic differentiation; T. spicata var. intricata; Bone regeneration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100310

In situ monitoring of mutagenicity of air pollutants in São Paulo City using Tradescantia-SHM bioassay
Provedor de dados: 52
Autores: Ferreira,Maria Izildinha; Rodrigues,Geraldo Stachetti; Domingos,Marisa; Saldiva,Paulo Hilário do N.
The Tradescantia stamen hair mutation assay (Trad-SHM) was employed for assessing the environmental mutagenesis in two areas of São Paulo City. The study sites were defined as follows: 1) Celso Garcia Avenue, an industrial area on the outskirts of the urban center (Belém neighborhood), with an average daily traffic volume of 8,750 vehicles; 2) Doutor Arnaldo Avenue, in downtown area (Pinheiros neighborhood), with a mostly residential and business offices occupation, with an average daily traffic volume of 46,125 vehicles. The negative control (background) site is located in the city of Jaguariúna, 138 km from São Paulo, in an area of low atmospheric pollution. The mutation frequency observed for Belém was similar to the background for clone 4430, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pollution; Mutagenesis; Bioassay; Tradescantia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200017

First record of partial albinism in two catfish species of Genidens (Siluriformes: Ariidae) in an estuary of Southern Brazil
Provedor de dados: 52
Autores: Leal,Mateus Evangelista; Schulz,Uwe Horst; Albornoz,Pablo Lehmann; Machado,Rodrigo; Ott,Paulo Henrique.
This study reports the first occurrence of partial albinism in two species of Ariidae: Genidens barbus and Genidens planifrons from an estuary of Southern Brazil. Possible causes of the simultaneous occurrence of three specimens with partial albinism are discussed, including random genetic alterations, chronic contamination effects and small effective population size, which may favor the expression of the recessive albino gene.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estuarine fishes; Genidens barbus; Genidens planifrons; Leucism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200008

Erratum: Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300904

Selection of candidate fish species for farming in the bay of Guaratuba, Brazil
Provedor de dados: 52
Autores: Bouchereau,Jean-Luc; Chaves,Paulo T.; Albaret,Jean-Jacques.
An inventory of the maximum length (ML) reached by 57 species of fishes living in the mangrove of Guaratuba, Brazil, was undertaken with an aim to evaluate the relationship between the ecosystem and the size of individuals. For each of these species, the maximum length found in the region were compared with those available in existing literature. The majority of populations presented individuals whose length reached at least 40% than the known maximum length for the species, although only 19.3% were longer than 300mm in absolute value. Population of the other species were represented only by individuals either in their initial development phases, or reduced length as compared to the maximum length known elsewhere for the same species. In this mangrove,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maximum length; Selection; Fish; Aquaculture; Mangrove.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100003

Biodiversity of Antimicrobial-Producing Actinomycetes Strains Isolated from Dry Dipterocarp Forest Soil in Northeast Thailand
Provedor de dados: 52
Autores: Chanthasena,Panjamaphon; Nantapong,Nawarat.
The increasing use of antibiotics has led to the development of drug-resistant microorganisms. The emergence of resistant strains has compromised the treatment and control of infectious diseases. As a result, the search and development of a novel class of antibiotic drugs are required. Actinomycetes have been recognized as a richest source of secondary metabolites including antibiotics. The aim of this study was to investigate the diversity of antibiotic-producing soil Actinomycetes from dry dipterocarp forest in Northeast of Thailand. The soil from this area has been known for its poor in nutrients and highly acidic with pH values around 4.5. In such a harsh condition, soil-inhabiting Actinomycetes elevate their protective mechanisms by inducing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soil bacteria; Antibiotics; Actinomycetes; Streptomyces.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100417

Arboreous vegetation of an alluvial riparian forest and their soil relations: Porto Rico island, Paraná river, Brazil
Provedor de dados: 52
Autores: Campos,João Batista; Souza,Maria Conceição de.
The dynamics of alluvial deposits in floodplains forms islands and sandbanks. Deposits frequently accumulate at the river margins and on islands with consequent side growths. One of these sandbanks which started to form in 1952 annexed an area of 12.4ha to the Porto Rico island (53º15’W and 22º45’S). At present a forest fragment of approximately 2.0 ha exists in this place. The structural analysis of arboreous vegetation of this fragment showed a floristic gradient related to the physical and chemical variations of the substratum. High density of pioneer species associated to the absence of recruitment of new individuals of these and other successional categories indicated that the forest was impaired in its succession process. This fact could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alluvial forest; Floodplain; Forest structure; Phytosociology; Riparian vegetation; Succession.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200004

Effects of oral L-carnitine and DL-carnitine supplementation on alloxan-diabetic rats
Provedor de dados: 52
Autores: Bazotte,Roberto Barbosa; Lopes-Bertolini,Gisele.
The effect of oral L-carnitine (LC) or DL-carnitine (DLC) supplementation during one or four weeks (200 or 400 mg.kg-1.day-1) in diabetic rats was investigated. After the supplementation period, the blood was collected for the evaluation of total (TC) and free L-carnitine (FC), glucose, total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triacylglycerol. Tissues were collected for the determination of TC and FC concentrations. The carnitine supplementation did not change levels of glucose, total cholesterol, HDL-C and LDL-C in the blood. Diabetic rats showed hypertriacylglycerolemia and decreased blood and tissue levels of FC and TC. Normalization of the blood triacylglycerol and increased blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carnitine; Diabetes; Dietary supplementation; Lipids; Rat.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100010

Identification and quantification of monomers released from dental composites using HPLC
Provedor de dados: 52
Autores: Archegas,Lucí Regina Panka; Rached,Rodrigo Nunes; Ignacio,Sérgio Aparecido; Vasconcelos,Eliane Carvalho de; Ramos,Débora Toledo; Souza,Evelise Machado de.
The aim of this study was to detect and quantify the main residual monomers released from composites, using high performance liquid chromatography (HPLC). Discs were made with dental composites (Herculite XRV, Tetric Ceram and Filtek Z250) and immersed in deionized water at 37ºC for 28 days, with water changes in 1, 7, 14 and 21 days. The mean concentration of residual monomers were subject to the Kruskal-Wallis test (p&lt;0.05). Tetric Ceram exhibited significantly higher concentrations of leached monomers. Bis-GMA was the monomer released in lower concentrations for all the materials. There was no statistical difference between the amounts of TEGDMA and UDMA. Most of the monomers demonstrated maximal concentration at the 7-day period. The HPLC analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental composites; Monomers; HPLC; TEGDMA; Bis-GMA; UDMA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400009

Influence of the gender on cerebral vascular diameters observed during the magnetic resonance angiographic examination of willis circle
Provedor de dados: 52
Autores: Stefani,Marco Antonio; Schneider,Felipe Luis; Marrone,Antonio Carlos Huf; Severino,Antonio Generoso.
The present study evaluated the calibers and anatomic configurations based on the Magnetic Resonance analysis (MRA), assaying the cerebral vascular territories and sex-linked variations. A randomized sample of 30 angiographic examinations in adult patients of both sexes was obtained and components of the circle of Willis were identified. Branch diameters were measured on a transversal cut 5mm from the vessel origin in a typical angiographic frontal incidence. For the comparative statistical analysis, tests were divided in the groups considering the patients' sex and age. The classical Circle of Willis configuration was oberved in only 15 samples (50%). Greater calibers were observed in the arteries of the posterior circulation and multiple linear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiography; Magnetic resonance; Anatomic variations.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100006

Transition metal rates in latosol twice treated with sewage sludge
Provedor de dados: 52
Autores: Pigozzo,Ana Tereza Jordão; Lenzi,Ervim; Luca Junior,Jorge de; Scapim,Carlos Alberto; Costa,Antonio Carlos Saraiva da.
Agricultural recycling of sewage sludge has been a source of accumulation of heavy metals in the environment which may reach toxic levels and cause serious damage to the biota. Field experiments were undertaken for two agricultural years (2000 and 2002) and effects of two sewage sludge applications were evaluated through the extraction of (essential and non-essential) transition metals by diethylenetriaminepentaacetic acid (DTPA) extractor in a medium texture dystrophic Dark Red Latosol. Cd, Ni, Co, Pb and Cr were not detected. Application of sewage sludge initially caused a slight pH rise in the soil; later pH lowered and kept itself close to the starting level. It could be concluded that through consecutive sludge application, extractable rates of Fe and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metals; Sewage sludge; DTPA; Biosolid; Nutrient rates.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400020

Influence of the temperature, pressure and viscosity on the oil measurement with turbine type measurers
Provedor de dados: 52
Autores: Damasceno,Magalí Araújo; Penha,Janaina Karla de Medeiros; Silva Junior,Nivaldo Ferreira da; Felipe,Raimundo Nonato B.; Felipe,Renata Carla Tavares dos Santos; Medeiros,Gilson Gomes de.
The flow measurement of liquids and gases is a necessity in many industrial applications. There is a great amount of measurers for such purpose, as, for example, the coriolis, positive displacement and type turbine measurers. A measurer sufficiently used for the oil flow measurement is the turbine type, because it uses the proper extracted energy of the measured flow for its functioning, moreover is also used as standard for the calibration of other measurers. For this reason, it is important to study the parameters that influence the measurement process for turbine measurers. In Brazil, to measure the volume of oil, regardless the type of measurer, it is necessary to observe "Portaria Conjunta Nº. 1", of June 19, 2000, that approved the Technical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oil; Measurement; Turbine.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200011

Enhancement of biohydrogen production from starch processing wastewater and further inside its ecosystem disclosed by 16S rDNA sequencing and FISH
Provedor de dados: 52
Autores: Sutthipattanasomboon,Chonlapin; Wongthanate,Jaruwan.
ABSTRACT Biohydrogen production from starch processing wastewater in this study resulted the highest yield of 61.75 mL H2/g COD at initial pH 7.0, thermophilic temperature, and iron concentration 800 mg Fe/L. The yield was 2-folded higher than the operation at mesophilic temperature or without iron addition. Cell immobilization by addition of biomaterials (BM) could improve the hydrogen yield by 2-folded comparing to the non-addition. BM from plants (loofa sponge) was found producing higher yield than that from animals (silk cocoon), and optimal concentration of BM was 5% (V/V). Furthermore, it was revealed further inside its ecosystem using SEM, 16S rDNA sequencing and FISH. There was found rod-shaped microorganisms of Bacillus cereus, which reported as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biohydrogen production; Starch processing wastewater; Biological supporting materials; Bacillus cereus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100600

Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
Provedor de dados: 52
Autores: Kumari,Annapurna; Mahapatra,Paramita; Banerjee,Rintu.
Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central composite design; Enterobacter aerogenes; Lipase; Optimization; Parameter; Response Surface Methodology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005

Biodegradable films made from raw and acetylated cassava starch
Provedor de dados: 52
Autores: Larotonda,Fábio D. S.; Matsui,Kátia N.; Soldi,Valdir; Laurindo,João B.
Studies were carried out to produce biodegradable films from cassava starch. Two alternatives were investigated. In the first, films were obtained by starch gelatinization followed by thermopressing and glycerol was used at different concentrations as a plasticizer. In the second, starch acetate films were obtained by solubilization of cassava starch acetate in organic solvents, followed by casting on a glass plate and drying at room temperature. The films obtained by gelatinization were transparent and resistant to traction. The starch acetate films were also transparent but breakable. The use of these starch acetate films in paper impregnation improved the mechanical properties of the paper.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Starch acetate; Films; Tensile strength.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300019

Effect of vacuum impregnation temperature on the mechanical properties and osmotic dehydration parameters of apples
Provedor de dados: 52
Autores: Paes,Sabrina Silva; Stringari,Gustavo Beulke; Laurindo,João Borges.
The effect of sucrose solution temperature on the mechanical properties, water loss (WL), solids gain (SG) and weight reduction (WR) of apples (Fuji var.) treated by vacuum impregnation was studied. Temperatures were varied from 10 to 50 ºC, using a sucrose solution of 50 ºBrix. The mechanical properties were studied throughout a stress relaxation test. The results showed that the SG varied between 10.57 and 14.29 % and the WL varied between 10.55 and 14.48 %. The treated fruit soluble solids increased with the temperature probably due to the lower viscosity of the solution. The maximum stress was highest at 10 ºC, decreasing at higher temperatures, probably due the softening of the structure.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vacuum; Impregnation; Temperature; Solids; Water; Texture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400018

Biofiltration of volatile organic compounds of Brazilian gasoline
Provedor de dados: 52
Autores: Rizzolo,Joana Antunez; Santos,Vanessa Cristina de Castro; Soares,Marlene; Woiciechowski,Adenise Lorenci; Soccol,Carlos Ricardo.
Gasoline vapors pollute the atmosphere and can be harmful to human and animal health. Tons of particles of this pollutant are expelled to the atmosphere, generating great economic losses to the industries and serious damage to the environment. Biofiltration is an option of simple technology with low costs that can be used for the gasoline vapor treatment. The present study was carried out in two biofilter systems of laboratorial scale. The biofilter system 1 was operated with a total volume of 2.72 L (four columns) and the biofilter 2 with 2.04 L (three columns) of total volume. Both of them were operated in sequence, with airflow of 450 mL entering each one. Results obtained were as follows for the removal efficiency (RE) of different gasoline vapor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofiltration; Volatile organic compounds; Gasoline; Atmospheric pollution.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100017

Studies on stem cuttings of kiwi (Actinidia chinensis PL. CV Bruno)
Provedor de dados: 52
Autores: Ono,Elizabeth O.; Rodrigues,João D.; Pinho,Sheila Z. de.
The work was carried out to study the effects of some auxins and boron in the rooting of kiwi (Actinidia chinensis Planch cv Bruno) stem cuttings.. These cuttings were treated on the base individually with H2O; NAA 300 mg.L-1; IBA 300 mg.L-1; NAA 300 mg.L-1 + Boron; IBA 300 mg.L-1 + Boron; NAA 0,5%-talc and IBA 0,5%-talc. After the treatments, the cuttings were placed in styrofoam trays with vermiculite under moist conditions for 120 days. The evaluation of auxin and boric acid effects were made by observing rooted stem cuttings percentage; reducing and total sugar analysis (g/100 g of dry matter); and tryptophan analysis (in µg/100 mg of dry matter). The effects of such treatments were observed during four seasons of the year. The results showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinidia chinensis; NAA; IBA; Boron; Rooting.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100007

Hepatoprotective efficacy of Hypnea muciformis ethanolic extract on CCl4 induced toxicity in rats
Provedor de dados: 52
Autores: Bupesh,Giridharan; Amutha,Chinnaiah; Vasanth,Sakthivel; Manoharan,Natesan; Raja,Ramalingam Senthil; Krishnamoorthy,Raju; Subramanian,Periyasamy.
The ethanolic extract of Hypnea muciformis (red algae) was tested for hepatoprotective activity against experimentally induced liver damage by Carbon tetrachloride (CCl4) in male albino rats. The levels of serum enzymatic and biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), Serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), lactate dehydrogenase, 5' nucleotidase, bilirubin, creatinine, urea, triglycerides, lipid peroxides and albumin were determined. The CCI4 induced lesions in the liver significant increased the levels of serum marker enzymes SGPT and SGOT, bilirubin, creatinine and decreased urea. The oral treatment with ethanolic extract of H. muciformis exhibited significant hepatoprotective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypnea muciformis; Hepatoprotective activity; Carbontetrachloride.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600008

Phenotypic Characterization and Synthesis of Extracellular Catecholase from a Newly Isolated Bacterium Pseudomonas sp. BSC-6
Provedor de dados: 52
Autores: Lugani,Yogita; Kauldhar,Baljinder Singh; Kaur,Navneet; Sooch,Balwinder Singh.
Abstract Catecholase (EC 1.10.3.1), an oxidoreductase enzyme is a key member of polyphenol oxidase family which catalyze the degradation of catechol. This enzyme possesses vast applications in diverse areas and is found in bacteria, fungi, mushrooms, higher plants, arthropods, amphibians and mammals. Catechol, a phenolic compound, is used as a starting material in the synthesis of various industrial compounds such as inhibitors, antioxidants, pesticides etc. The release of this phenolic compound in the environment causes toxicity to both flora and fauna. In the present studies, emphasis has been laid on isolation, screening and characterization of catechol degrading bacterium coupled with synthesis of catecholase enzyme. Further, the selected isolated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catecholase; Catechol; Polyphenol oxidase; Pseudomonas sp. BSC-6; Bioremediation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100216

Preparation of biological fish silage and its effect on the performance and meat quality characteristics of quails (Coturnix coturnix japonica)
Provedor de dados: 52
Autores: Ramírez,José Carmen Ramírez; Ibarra,José Inés; Romero,Francisco Arce; Ulloa,Petra Rosas; Ulloa,José Armando; Matsumoto,Keiko Shirai; Cordoba,Belinda Vallejo; Manzano,Miguel Ángel Mazorra.
The aim of the present study was to produce fish silage by lactic acid fermentation and evaluate its use in feeding of quails (Coturnix coturnix japonica). An oven-dried mixture of fish silage and soybean meal (1:1 w/w) was used to prepare the diets with different levels of inclusion (0, 10, 20 and 30%) and evaluate its effect on the performance and meat quality of 160 quails. The inclusion level did not affect the growth and feed conversion ratio. The carcass yield (70.3%) and sensory quality of breast meat were not significantly different among the treatments (p&amp;gt;0.05). However, the concentration of unsaturated fatty acids such as oleic (C18:1n9C), linoleic (C18:2n6C), linolenic (C18:3n3), arachidonic (C20:4n6), cis eicosapentaenoic (C20:5n3) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish wastes; Biological silage; Quails feeding; Meat quality.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600016

Protection of Grid Connected Photovoltaic Systems (GCPVS)
Provedor de dados: 52
Autores: Silva,Jardel Eugenio da; Gouveia,Danilo Carvalho de; Urbanetz Junior,Jair.
ABSTRACT Installations of photovoltaic systems connected or not to the electrical network have become increasingly popular, but it is often carried out by unqualified people using low quality components. The present study aims to describe the methodology adopted for the protection of grid connected photovoltaic systems (GCPVS) and the implications caused by their lack. Photovoltaic modules are typically installed in galvanized, painted, stainless aluminum or steel structures, which must be connected to a common ground, as these structures and any other components of the system could be energized by the photovoltaic array and may cause serious injuries or even death during routine maintenance, these fatalities can still result in ramifications for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic systems; Grounding; Photovoltaic modules; Protection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200225

Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS
Provedor de dados: 52
Autores: Bittencourt-Oliveira,Maria do Carmo; Cunha,Maristela Casé Costa; Moura,Ariadne do Nascimento.
The escalating occurrence of cyanobacterial toxic blooms demands a better understanding of genetic variability as an auxiliary expedient in species identification, collaborating with the monitoring of water destined to public supply. This study aimed at the unraveling of genetic polymorphism in the toxic and nontoxic strains of Microcystis (Cyanobacteria) species, isolated from diverse Brazilian localities through the RFLP-PCR technique applied to the c-phycocyanin encoding operon and its intergenic spacer (cpcBA-IGS). Eighteen strains belonging to M. aeruginosa, M. panniformis, M. protocystis and M. wesenbergii, plus two other unidentified strains, were analyzed by means of the morphological and molecular data. The molecular data constituted three groups...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyanobacteria; CpcBA-IGS; Genetic polymorphism; Microcystin; Microcystis; RFLP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400014

Endophytic Microbiota Associated with the Root Tips and Leaves of Baccharis dracunculifolia
Provedor de dados: 52
Autores: Santana,Raiana S. M.; Fernandes,G. W.; Ávila,Marcelo P.; Reis,Mariana P.; Araújo,Flávio M. G. de; Salim,Anna C. M.; Oliveira,Guilherme; Chartone-Souza,Edmar; Nascimento,Andréa M. A.
ABSTRACT Although endophytic bacteria impact the health, and ultimately the fitness, of their hosts, our understanding of the diversity of endophytic species remains limited. Here we report on the endophytic microbiota inhabiting the roots, healthy leaves and leaves attacked by a gall-inducing insect of Baccharis dracunculifolia, a species of major economic relevance in South America, using 16S rRNA gene new generation sequencing. Rhodoplanes and Nitrospira were well represented in the communities of roots and leaves; known to be important for nitrogen cycling. The difference in bacterial diversity between healthy and galled leaves was not pronounced. The leaves seem to harbor specialized bacteria with high tolerances to abiotic stresses such as wide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baccharis dracunculifolia; Endophytic bacteria; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100433

Adiabatic differential scanning calorimetric study of divalent cation induced DNA - DPPC liposome formulation compacted for gene delivery
Provedor de dados: 52
Autores: Süleymanoglu,Erhan.
Complexes between nucleic acids and phospholipid vesicles have been developed as stable non-viral gene delivery vehicles. Currently employed approach uses positively charged lipid species and a helper zwitterionic lipid, the latter being applied for the stabilization of the whole complex. However, besides problematic steps during their preparation, cationic lipids are toxic for cells. The present work describes some energetic issues pertinent to preparation and use of neutral lipid-DNA self-assemblies, thus avoiding toxicity of lipoplexes. Differential scanning calorimetry data showed stabilization of polynucleotide helix upon its interaction with liposomes in the presence of divalent metal cations. It is thus possible to suggest this self-assembly as an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleic acid-phospholipid interactions; Divalent cation-induced complex formation; Phase transitions; Microcalorimetry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600007

The effect of management of transport and lairage conditions on broiler chicken breast meat quality and DOA (Death on Arrival)
Provedor de dados: 52
Autores: Oba,Alexandre; Almeida,Mauricio de; Pinheiro,João Waine; Ida,Elza Iouko; Marchi,Denis Fabricio; Soares,Adriana Lourenço; Shimokomaki,Massami.
The aim of this work was to evaluate the influence of time of broiler chicken transportation and lairage prior to slaughtering on the occurrence of PSE (Pale, Soft, Exudative) meat and Death On Arrival (DOA) under non-commercial conditions in the Brazilian summer. Male birds (n=250) from a commercial line were subjected to different periods of journey (30, 90, and 180 min) and lairage (0, 90, and 180 min) before slaughtering. The occurrence of PSE was higher in broilers subjected to shorter journeys and lairage periods, whereas DOA was more pronounced upon longer periods of transport and lairage. The DOA occurrence percentage was much higher in comparison to commercially available figures, confirming that broiler chickens are very sensitive to both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water holding capacity; PSE meat; Color abnormalities; Broiler management.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700026

Effect of coastline properties and wastewater on plankton composition and distribution in a stressed environment on the north coast of Olinda-PE (Brazil)
Provedor de dados: 52
Autores: Pereira,Luci Cajueiro Carneiro; Jiménez,José Antonio; Koening,Maria Luise; Porto Neto,Fernando F.; Medeiros,Carmen; Costa,Rauquírio Marinho da.
A study of the structure of the plankton community along the coastal zone of the Casa Caiada-Rio Doce area (Pernambuco, Brazil) is presented. The results obtained indicated that in the protected zones the water presented high turbidity, high temperature, low salinity and high nutrient and oxygen rates. All of these conditions were responsible for the reduction in plankton diversity and an increase in the biomass of the most opportunistic (r-strategist) species e.g. Gyrosigma balticum and Bacillaria paxillifera (Phytoplankton) and Euterpina acutifrons (Zooplankton). The differences found with respect to others area studies performed in pristine conditions indicated that plankton structure could be used as an environmental health indicator and that, in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plankton; Sheltered areas; Environmental indicators.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800018

Pharmacodynamic and Pharmacokinetic Studies of β-Cyclodextrin:Dexamethasone Acetate Complexes in Mice
Provedor de dados: 52
Autores: Rodrigues,Patrik Oening; Becker,Ana Cláudia; Dellagnelo,Viviane Aparecida; Benetti,Cibele Nabhan; Pereira,Eduardo Manoel; Wagner,Theodoro Marcel; Silva,Marcos Antonio Segatto.
The aim of this study was to evaluate the in vivo activity of the anti-inflammatory and analgesic effects of a suspension of the complex composed of dexamethasone acetate (DMA) with β-cyclodextrin in comparison to a suspension of the pure DMA. Solid complexes prepared by different methods were evaluated in pharmacodynamics and pharmacokinetics studies. The pharmacodynamic effect was investigated although the capacity of the inhibited the inflammation. Models of abdominal constriction, carrageenan-induced paw oedema and formalin induced licking were used. The study of the pharmacodynamic comparison of free DMA and products of β-CD:DMA demonstrated no significant difference in the majority of the tests performed. Plasma concentrations of DMA and DMA:β-CD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solubility; Dexamethasone acetate; Β-cyclodextrin; Bioavailability; Anti-inflammatory activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600887

Mechanism of action of probiotics
Provedor de dados: 52
Autores: Hemaiswarya,S; Raja,R.; Ravikumar,R.; Carvalho,Isabel S.
The modern diet doesn't provide the required amount of beneficial bacteria. Maintenance of a proper microbial ecology in the host is the main criteria to be met for a healthy growth. Probiotics are one such alternative that are supplemented to the host where by and large species of Lactobacillus, Bifidobacterium and Saccharomyces are considered as main probiotics. The field of probiotics has made stupendous strides though there is no major break through in the identification of their mechanism of action. They exert their activity primarily by strengthening the intestinal barrier and immunomodulation. The main objective of the study was to provide a deep insight into the effect of probiotics against the diseases, their applications and proposed mechanism of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-allergic; Gastrointestinal problems; Immunomodulation; Infectious diseases; Probiotics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100015

Caffeine formation by suspension cultures of Coffea dewevrei
Provedor de dados: 52
Autores: Sartor,Rosana Mary; Mazzafera,Paulo.
The low caffeine content in leaves of C. dewevrei (~ 0.5 mg/g) is due to a low biosynthesis associated with a fast degradation. On the other hand, high biosynthesis and low degradation confer a higher content (~ 8 mg/g) in leaves of C. arabica. In this work it was observed that cell cultures of C. dewevrei recovered the ability to synthesize caffeine almost in similar levels of C. arabica cultures. Tracer experiments with labelled carbon dioxide showed a significant accumulation of radioactivity in caffeine and metabolites, indicating an active biosynthesis. When the cultures were fed with labelled caffeine most of the radioactivity was recovered in caffeine, indicating that although active, degradation was not so efficient as in leaves, and therefore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Caffeine; Cell suspension culture; Methylxanthines.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100009

Research on the space environment characteristic and the development of the traditional village in China: Taking Pengzhuang in the northern Jiangsu province as an example
Provedor de dados: 52
Autores: Yao,Peng.
ABSTRACT Traditional villages in the northern Jiangsu province have a local character and history unique artistic style, traditional culture and art Jiangsu indispensable part. How to make achieving sustainable and harmonious for development in rural areas, it is a major problem currently in these places. In this paper, field research and theoretical analysis a multidisciplinary approach, the traditional villages of northern Jiangsu space environment features, including location and spatial pattern are elaborated, analyzed the current conditions of rural development. Then taking Pengzhuang Village in the northern Jiangsu as example, discuss the current situation as well as its architectural characteristics of the space environment group and architectural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Traditional villages; Space environment; Characteristic; Ecological; Development.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200202

Analysis of the 40K levels in soil using gamma spectrometry
Provedor de dados: 52
Autores: Santos Júnior,José Araújo dos; Cardoso,Jorge João Ricardo Ferreira; Silva,Cleomacio Miguel da; Silveira,Suêldo Vita; Amaral,Romilton dos Santos.
Potassium-40 was determined in soil in an area with 40,000 m² situated in the western State of Pernambuco, Brazil. For radiometric measurements, the gamma spectrometry method with a high purity germanium (HPGe) detector was used. Sampling of 78 soil samples has been performed at intervals of 25 m. The specific activities of 40K were calculated based on the photopeak of 1.46 MeV. Values from 541 to 3,572 Bq kg-1 were obtained (mean of 1,827 Bq kg-1). These values allowed the determination of the elemental concentrations as well as the absorbed dose rates in air, 1 m above the ground. The values varied from 1.7 to 11.5% (mean of 6%) and from 23.4 to 154.3 nGy h-1 (mean of 79 nGy h-1), respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 40K in soil; 40K gamma spectrometry; 40K in environmental radiological protection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700033

Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
Provedor de dados: 52
Autores: Khan,Abdul Basit; Khattak,Mehmood Iqbal; Tarar,Omer Mukhtar; Habib,Farzana; Jamil,Khalid; Yasmin,Arfa; Parvez,Shoukat.
In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxyalkanoates; Biodegradable polymers; PHA synthase gene; FTIR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400015

Co-production of Proteases and Bioactive Protein Hydrolysates from Bioprocessing of Feather Meal
Provedor de dados: 52
Autores: Bernardo,Bernardete da Silva; Ramos,Rodrigo Ferraz; Callegaro,Kelly; Daroit,Daniel Joner.
Abstract: Feather meal conversion through submerged cultivations with Bacillus strains (CL33A, CL14) yielded proteases and protein hydrolysates. After 4-day (CL33A) and 10-day (CL14) cultivations, protease activities reached 461 U/mL; hydrolysates presented antioxidant (radicals-scavenging, 57-77%; Fe2+-chelation, 14-28%; Fe3+-reduction) and antidiabetic (dipeptidyl peptidase-IV inhibition, 49-52%) potentials. The obtained bioproducts present prospective commercial/industrial applications.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Bioprocess; Enzyme; Antioxidant; Antidiabetic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100220

Morphogenic variability of some autochthonous plum cultivars in western Serbia
Provedor de dados: 52
Autores: Milosevic,Tomo; Milosevic,Nebojsa; Mratinic,Evica.
A study conducted over a period of two years in a Western Serbian valley included in situ identification of autochthonous plum cultivars. Observation and recording of their phenological and pomological traits were performed using IBPGR and UPOV methodologies. Fifteen cultivars derived from Prunus domestica L. and two cultivars derived from P. insititia L. were identified. Flowering started between 24 March and 7 April and fruit ripening between 12 July (Petrovača) and 16 September (Trnovača). Fruit weight ranged from 6.79± 0.018 to 36.62± 0.251 g and stone weight from 0.14± 0.002 to 1.95± 0.023 g. The cultivars were classified as being extremely small in terms of fruit size. Rounded fruit shape and light-yellow ground colour were dominant. Skin colour...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fruit; Genetic bases; Germplasm; Prunus domestica L.; Prunus insititia L.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600005

Microscopic Studies on Erythrocytes of Channa punctata Exposed to Commercial Grade Lindane
Provedor de dados: 52
Autores: Bhattacharjee,Debasish; Das,Suchismita.
ABSTRACT Light and scanning electron microscopic studies of erythrocytes of Channa punctata exposed to two sublethal doses of lindane over a period of 21 days revealed myriads of anomalies. High frequency of micronucleus appeared progressively in the erythrocyte, ranging from 3.8-7.5%, when monitored weekly for 21 d at 0.25 g/L and 1.0-3.0% at 0.025 g/L lindane. Prominent structural anomalies of erythrocytes included abnormal shapes, vacuolation, membrane invagination and disintegration. At higher dose and at later duration, a unique tendency of abnormal cells to stick together was observed. It could be concluded that commercial formulation of lindane could induce genotoxicity and structural anomalies in the erythrocytes of fish.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RBC; SEM; Light microscopy; Micronucleus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100449

Controle de flebotomíneos com DDT, em àrea endêmica de leishmaniose tegumentar no estado do Paraná, sul do Brasil
Provedor de dados: 52
Autores: Teodoro,Ueslei; Galati,Eunice A.B.; Kühl,João Balduíno; Lozovei,Ana Leuch; Barbosa,Orlando Carlos.
Evaluation of DDT insecticide used to sand flies control in domiciles and domestic animal shelters in an endemic area of cutaneous leishmaniasis. Collections of sand flies were made with Falcão traps in houses, in one pigsty and in one tree in Jussara Farm, municipality of Jussara, State of Paraná, Brazil, between 10 p.m. and 2 a.m. during 7 days from March 24 to April 1, 1992. In May, 1992 DDT insecticide (Dicloro Difenil Tricloro Etano) (1 g/square meter of area) was applied internally and externally on houses and in pigsty. After this, new collections of sand flies were made from July 1992 to February 1993, once a month, with Falcão traps installed in the same hour and conditions mentioned above. Before DDT insecticide application 12,960 sand flies were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Leishmaniose; Controle de flebotomíneos; DDT.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300014

Analysis of Maceaene and Macamide Contents of Petroleum Ether Extract of Black, Yellow, and Purple Lepidium Meyenii (Maca) and Their Antioxidant Effect on Diabetes Mellitus Rat Model
Provedor de dados: 52
Autores: Qiu,Congyang; Zhu,Tonglin; Lan,Liying; Zeng,Qingmao; Du,Zhiguo.
ABSTRACT Maceaene and macamide contents as well as antioxidant effect of petroleum ether extract of black maca (BM), yellow maca (YM), and purple maca (PM) on diabetes mellitus (DM) rats were investigated. The results showed that seven, six, and five analogues of macamides were identified from the petroleum ether extracts of BM, YM, and PM, respectively. BM extract exhibited the highest contents of total macamides. Comparatively, the PM extract has the lowest macamide quantity. The maceaene contents in all the extracts showed no significant difference (p&gt;0.05). Macamide contents in maca with the same color were not statistically different. Pharmacological results showed that 60-day oral administration of the petroleum ether extract of maca (100 mg/kg.d)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maca extract; Antioxidant activity; Maceaene; Macamide; Diabetes mellitus rat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100326

The immunogenicity and anti-tumor effects of a lung cancer DNA vaccine harboring a MUC-1 and GM-CSF fusion gene
Provedor de dados: 52
Autores: Yang,Zhen; Jiang,DanDan; Zhu,Qiang; Xiao,BinBin; Chen,Liang An.
ABSTRACT DNA vaccines have been shown to be an effective approach to induce antigen-specific cellular and humoral immunity. However, the inability of DNA vaccines to elicit strong immune responses in clinical trials limits the application of DNA vaccines. Here, we developed a new DNA vaccine based on MUC1, which has been suggested as a potential target for lung cancer therapy, and we enhanced the potency of the DNA vaccine by including granulocyte-macrophage colony-stimulating factor (GM-CSF) as an adjuvant. A series of DNA plasmids encoding MUC1, human GM-CSF and their conjugates were constructed and injected into female mice intramuscularly (i.m.). This action was followed by an electric pulse. The humoral and cellular immune responses after immunization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MUC1; GM-CSF; Lung cancer; DNA vaccine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100331

Trends in Mean Annual Streamflows in Serra da Mantiqueira Environmental Protection Area
Provedor de dados: 52
Autores: Vilanova,Mateus Ricardo Nogueira.
The aim of this study was to detect trends in the mean annual streamflow in watersheds of Serra da Mantiqueira Environmental Protection Area, an important Brazilian conservation area located between Minas Gerais, São Paulo and Rio de Janeiro States. Historical series of four selected streamgage stations were analyzed for the periods of 1980-1998 and 1980-2009, using the Mann-Kendall and Regional Mann-Kendall tests. The results showed that the mean annual streamflows of Serra da Mantiqueira Environmental Protection Area watersheds did not change during the complete period of 1980-2009. The tests detected statistically significant trends of reduction of mean annual streamflow during the 1980-1998 period. The confrontation of this result with the inexistence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streamflow changes; Serra da Mantiqueira Range; Mann-Kendall test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000601004

Efeito do armazenamento sobre as propriedades tecnológicas da farinha, de variedades de trigo cultivadas no Brasil
Provedor de dados: 52
Autores: Pirozi,Mônica R.; Germani,Rogério.
The aim of this work was to evaluate changes in technological properties of newly milled flours of BR-23, BR-35 and Anahuac varieties (Brazilian grown wheat) during storage for 180 days. Quality of samples was analysed for their rheological properties, acidity, falling number, glutomatic test and baking test, after periods of 0, 7, 15, 30, 60, 90, 120, 150 and 180 days. Most evident changes were the increase in flour acitidy and dough elasticity. The other characteristics did not show expressive changes. The flour of Anahuac variety was less influenced by the storage than the other ones. The results showed an increment in the flour quality, during 60-90 days of storage, althoug the baking test did not show expressive changes during all the period of storage.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Farinha de trigo; Qualidade tecnológica; Armazenamento; Maturação.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100015

Comparative Cytogenetics Among Three Sympatric Hypostomus Species (Siluriformes: Loricariidae): An Evolutionary Analysis in a High Endemic Region
Provedor de dados: 52
Autores: Lorscheider,Carla Andreia; Oliveira,Jordana Inácio Nascimento; Dulz,Thais Aparecida; Nogaroto,Viviane; Martins-Santos,Isabel Cristina; Vicari,Marcelo Ricardo.
ABSTRACT The karyotypes of three armored catfish species (Loricariidae) from the Iguaçu river, southern of the Brazil, were compared using different techniques: C-banding, Ag-NOR and fluorescence in situ hybridization (FISH), which used 5S and 18S rDNAs and total Cot-1 fraction as probes. Hypostomus commersoni and Hypostomus derbyi presented 2n = 68 chromosomes, with karyotype formulae 12m+12sm+14st+30a and 12m+12sm+10st+34a, respectively; whereas Hypostomus myersi presented 2n = 74 chromosomes and 12m+16sm+12st+34a. The chromosomal localization of the Ag-NORs, 5S and 18S rDNAs differed in number of sites and chromosomal localization among the studied species. The total Cot-1 probe permitted the visualization of the repetitive DNA fraction in karyotypes of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Armored catfish; FISH; Iguaçu river; Karyotype evolution.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100435

Corn performance with late sowing in Planalto Catarinense, Brazil, simulated with CERES-maize model
Provedor de dados: 52
Autores: Cardoso,Célio Orli; Soccol,Olívio José.
This work had the objective to investigate the delay effects in the sowing date on corn establishment, cycle duration and yield through the computer simulations using CERES-Maize model, in order to help agrarian producers from the Mountainous Area of Santa Catarina, Brazil to take better decisions on it. The computer simulations showed that: 1)the risks on corn establishment increased due to the delay of sowing during the studied years; 2) in some years, its cycles were drastically affected for the lower temperatures; 3) the cycle durations were longer when confronted with the low temperatures; 4) corn income decreased when the cycles were confronted with the low temperatures, water deficiency and low solar radiation; 5) potential incomes averaged 4944 kg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simulation; Mathematical Model; Sowing date.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400002

Genetic Engineering In BioButanol Production And Tolerance
Provedor de dados: 52
Autores: Rao,Ashok; Sathiavelu,A.; Mythili,S.
ABSTRACT The growing need to address current energy and environmental problems has sparked an interest in developing improved biological methods to produce liquid fuels from renewable sources. Higher-chain alcohols possess chemical properties that are more similar to gasoline. Ethanol and butanol are two products which are used as biofuel. Butanol production was more concerned than ethanol because of its high octane number. Unfortunately, these alcohols are not produced efficiently in natural microorganisms, and thus economical production in industrial volumes remains a challenge. The synthetic biology, however, offers additional tools to engineer synthetic pathways in user-friendly hosts to help increase titers and productivity of bio-butanol. Knock out...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butanol; CRISPR; Biofuel; Clostridium acetobutylicum.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100604

Ethanol affects the absorption and tissue distribution of orally administered antigens in mice
Provedor de dados: 52
Autores: Oliveira,Flávia Márcia; Gusmão,Leandro José; Cardoso,Valbert do Nascimento; Ramaldes,Gilson Andrade; Ruiz-de-Souza,Valéria; Gontijo,Cristiano Machado.
The aim of this work was to evaluate the effects of ethanol on the adsorption and tissue distribution of orally administered antigens in mice. Results showed that ethanol reduced the level of anti-ovalbumin IgA antibodies in intestinal fluid for the mice treated orally with a palmitoyl-ovalbumin conjugate. Ethanol was administered intragastrically to mice at 5 g/kg body weight for 14 days (chronic treatment), or 10 g/kg body weight every 7th day up to 14 days (acute treatment). Thereafter, 99m technetium-labeled antigens were administered and lymphoid tissues were collected. Ethanol interfered with the transport of ovalbumin to the liver. Moreover, the transport of palmitoyl-ovalbumin to mesenteric lymph nodes was reduced 6 h after the antigen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Absorption; Biodistribution; Technetium; Immune response.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400008

Occurrence of Diaretiella rapae (Mc´Intosh, 1855) (Hymenoptera: Aphidiidae) Parasitising Lipaphis erysimi (Kaltenbach, 1843) and Brevicoryne brassicae (L. 1758) (Homoptera: Aphididae) in Brassica napus in Mato Grosso do Sul
Provedor de dados: 52
Autores: Mussury,Rosilda Mara; Fernandes,Wedson Desidério.
The occurrence of Diaretiella rapae parasitising Lipaphis erysimi and Brevicoryne brassicae in canola field (Brassica napus) was evaluated through two sample methods in Dourados-MS. The methods, used weekly, were: entomologic sweep net and plants sacking. The aphids population was observed from initial to the senescence plant development. Aphids were more abundant during the flowering phase, and they were usually located in the stems of the inflorescence and development fruits. In this phase the largest parasitism level for D. rapae (89,7%) occurred. The sample method with a sweep net captured significantly (t=4,484, P &lt;= 0,01) more D. rapae while sacking method captured more parasitise aphids (t=2,199 with P &lt;= 0,05) and active aphids (t=3,513, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canola; Brassica napus; Diaretiella rapae; Parasitism; Lipaphis erysimi; Brevicoryne brassicae.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100007

Canopy gap colonization in the Atlantic Montane Rain Forest
Provedor de dados: 52
Autores: Lima,Renato A. Ferreira de; Moura,Leila Cunha de.
In the Atlantic Montane Rain Forest of South-eastern Brazil, a study was carried out to describe and evaluate canopy gap colonization. Gap composition by herb species was assessed through their soil coverage and woody species by measuring and identifying all individuals taller than one meter. Gap structure (gap size, number and diameter of treefalls), topographic position and surrounding vegetation were also measured. Two genera of Marantaceae were markedly frequent and abundant inside gaps. The more abundant and frequent woody species were shade tolerant. Species-rich families found inside gaps did not differ from the forest as a whole. Results revealed that direct and indirect influences of topography were important to determine gap composition of woody...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gap floristics; South-eastern Brazil; Marantaceae species; Montane rain forest; Topography.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700013

Anti-staphylococcal effectiveness of nisaplin in refrigerated pizza doughs
Provedor de dados: 52
Autores: Freitas,Wilma Carla de; Souza,Evandro Leite de; Sousa,Cristina Paiva de; Travassos,Antônio Eustáquio Resende.
This study evaluated the effectiveness of nisaplin, commercial product having nisin as active component, in decreasing the staphylococcal population in refrigerated pizza doughs. The refrigerated pizza dough pieces randomly chosen were dipped in the solutions with nisaplin concentrations of 1.0 x 10-3 g and 1.0 x 10-2 g nisaplin/mL named for the treatment A and B and kept under refrigeration (7 °C ± 1 °C). On times 0, 15 and 30 days post treatment the Staphylococcus spp. count was carried out. The results showed that both nisaplin treatments were able to reduce the Staphylococcus spp. count (CFU/g) in the refrigerated pizza doughs. However, only treatment B showed statistically significant reducer effect (p &lt; 0.05) on the count providing a decrease of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nisaplin; Nisin; Dough; Staphylococcus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300021

Monitoring and evaluation of need for nitrogen fertilizer topdressing for maize leaf chlorophyll readings and the relationship with grain yield
Provedor de dados: 52
Autores: Silva,Maria Anita Gonçalves da; Muniz,Antonio Saraiva; Mannigel,Anny Rosi; Porto,Simone Maria Altoé; Marchetti,Marlene Estevão; Nolla,Antonio; Grannemann,Ivan.
The study was carried out for two years in maize in succession to the wheat using no tillage system in a distroferric Red Latosol (Hapludox). Methods of management nitrogen fertilizer (120 kg ha-1) with ammonium sulphate were studied; the fertilizer was applied in maize sowing or in maize topdressing, and N with previous application in wheat sowing. In addition, leaf chlorophyll reading was used as an indicator for the need for topdressed nitrogen fertilizer. Nitrogen supply index (NSI) was shown to be effective at predicting need for topdressed nitrogen fertilizer for maize. The application of N improved the yield of the maize independent of the management system. The flowering stage was carried out at the appropriate time in order to estimate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrogen fertilized; Leaf chlorophyll; Crop rotation; No till; Zea mays; Triticum aestivum.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400004

Morphological and molecular identification of filamentous fungi isolated from cosmetic powders
Provedor de dados: 52
Autores: Pinto,Flavia Cristina Jastale; Lima,Daniel Braga de; Agustini,Bruna Carla; Dallagassa,Cibelle Borba; Shimabukuro,Maria Fernanda; Chimelli,Márcio; Brand,Debora; Fadel-Picheth,Cyntia Maria Telles; Bonfim,Tania Maria Bordin.
Seven fungi were isolated from 50 samples of cosmetic powders. Morphological analyses and ribosomal DNA Internal Transcribed Spacers sequencing were performed which allowed the discrimination of the isolated fungi as Aspergillus fumigatus, Penicillium sp., and Cladosporium sp. which could have, among their species, potentially pathogenic microorganisms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial contamination; Ribosomal DNA; Cosmetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600013

The p38 MAPK and NF-κB Pathways are Involved in Cyclic Compressive Force-induced IL-6 Secretion in MLO-Y4 Cells
Provedor de dados: 52
Autores: Chen,Xiwen; Chen,Wenchuan; He,Yun; Zhang,Yixin; Chen,Chenfeng; Zhu,Zhimin; Wang,Hang.
ABSTRACT We previously revealed the involvement of extracellular regulated protein kinases 1/2 (ERK1/2) in interleukin-6 (IL-6) secretion induced by cyclic compressive force (CCF) in MLO-Y4 cells. In this study, we investigated the contributions of the p38 mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways to IL-6 secretion by stimulating MLO-Y4 cells with CCF. At 80% confluence, different magnitudes (1000μstrain, 2000 μstrain and 4000 μstrain), frequencies (0.5 Hz, 1.0 Hz and 2.0 Hz) and durations (10 min, 30 min, 1 h, 3 h and 6 h) of CCF were loaded onto cells using a four-point bending system. Flow Cytometry (FCM) analysis was used to analyze cell mortality rates after CCF loading. p38 and p65 phosphorylation as well as IκBα...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-6; Cyclic compressive force; P38 mitogen-activated protein kinases; Nuclear factor-κB; Osteocyte.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100437

Bioactive Compounds and Antioxidant Activity in Leaves of Endemic and Native Isatis spp in Turkey
Provedor de dados: 52
Autores: Comlekcioglu,Nazan.
Abstract This study was undertaken to evaluate the health-promoting potentials of Isatis aucherii, Isatis buschiana, Isatis candolleana, Isatis tinctoria subsp. corymbosa and Isatis tinctoria. I. aucherii and I. candolleana are endemic, I. buschiana, I. tinctoria subsp. corymbosa are native in Turkey. While I. tinctoria is a well studied species, there is insufficient information about other endemic and native species. Therefore, this study is focused to reveal the bioactive compounds of poorly studied endemic and native species. In this context, protein, ash, glucosinolates, fatty acids, total phenolic and flavonoid content, and antioxidant activities were determined in leaf extracts. The highest protein and fatty oil contents were observed in I....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant activity; Fatty acids; Flavonoid; Glucosinolate; Isatis; Phenolic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100411

Unraveling the Role of Π - Conjugation in Thiophene Oligomers for Optoelectronic Properties by DFT/TDDFT Approach
Provedor de dados: 52
Autores: Gajalakshmi,; Tamilmani,V.
ABSTRACT Thiophene oligomer has been investigated using DFT/TDDFT calculations with an aim to check its suitability for opto electronic applications and also to analyse the influence of π-bridge. Our results revealed that thiophene oligomers have excellent π-conjugation throughout. FMO analysis give an estimate of band gap of thiophene oligomer and further revealed HOMO are localized on π - bridge, donor group and LUMO are localized on π - bridge and acceptor group. A TDDFT calculation has been performed to understand the absorption properties of them in gas phase and solvent phase. PCM calculations convey that absorption maxima show positive solvatochromism. Among the designed candidates, the one with more π - bridge show higher wavelength of absorption...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thiophene oligomer; Π - bridge; TDDFT; PCM; NBO.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300400

Escherichia Coli: Characteristics of Carbapenem Resistance and Virulence Factors
Provedor de dados: 52
Autores: Candan,Esra Deniz; Aksöz,Nilüfer.
ABSTRACT In this study, fifty Escherichia coli strains were analyzed by multiplex polymerase chain reaction for the genes expressed carbapenemase and virulence factors in order to determine the presence of carbapenemase and nine virulence factors and investigate the association between these two characteristics. When carbapenemase susceptibility was taken into consideration, OXA-48 type carbapenemase was determined for 22% of the total strains. Also, the frequency of virulence gene regions in E.coli infections and virulence gene profiles of these isolates were examined and the frequency of pap, afa, sfa, fimA, iroN, aer, iutA, hly and cnf-1 genes were 24, 38, 20, 84, 28, 90, 92, 10 and 34% respectively. A significant correlation was found between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbapenemase; Escherichia coli; Multiplex polymerase chain reaction; Virulence factor.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100415

Biodistribution of the radiopharmaceutical sodium pertechnetate after biliopancreatic bypass with a duodenal switch
Provedor de dados: 52
Autores: Araújo-Filho,Irami; Rêgo,Amália Cínthia Meneses; Brandão-Neto,José; Villarim-Neto,Arthur; Egito,Eryvaldo Sócrates Tabosa; Azevedo,Ítalo Medeiros; Medeiros,Aldo Cunha.
Study with the purpose to examine the effects of duodenal switch (DS), regularly performed in morbidly obese patients, on biodistribution of sodium pertechnetate in several organs of rats. There was no early or late mortality in either rats groups. The values of percent radioactivity per gram of tissue (%ATI/g), showed no significant difference in liver, stomach, small bowel, duodenum, kidney, heart, bladder, bone and brain, when compared the DS rats with sham and controls rats. A postoperative significant increase (p&lt;0.05) in mean %ATI/g levels was observed in spleen, pancreas and muscle in group DS rats, as compared to group S and C rats. In the lung there was an increase and in thyroid a decrease in mean %ATI/g of DS rats, when compared to sham rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bariatric surgery; Duodenal switch; Sodium pertechnetate; Biodistribution.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600024

Factors influencing crude oil biodegradation by Yarrowia lipolytica
Provedor de dados: 52
Autores: Ferreira,Tatiana Felix; Coelho,Maria Alice Zarur; Rocha-Leão,Maria Helena Miguez da.
Yarrowia lipolytica is unique strictly aerobic yeast with the ability to efficiently degrade hydrophobic substrates such as n-alkenes, fatty acids, glycerol and oils. In the present work, a 2(4) full factorial design was used to investigate the influence of the independent variables of temperature, agitation, initial cell concentration and initial petroleum concentration on crude oil biodegradation. The results showed that all variables studied had significant effects on the biodegradation process. Temperature, agitation speed and initial cell concentration had positive effects, and initial petroleum concentration had a negative effect. Among the crude oil removal conditions studied, the best temperature and agitation conditions were 28ºC and 250 rpm,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yarrowia lipolytica; Crude oil; Biodegradation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500019

Thermic Variation in Incubation and Development of Pantanal Caiman (Caiman crocodilus yacare) (Daudin, 1802) Kept in Metabolic Box
Provedor de dados: 52
Autores: Miranda,Maristela Parra; Moraes,Gentil Vanini de; Martins,Elias Nunes; Maia,Luis Carlos Pinto; Barbosa,Orlando Rus.
Thirty-two eggs of Pantanal caiman (Caiman crocodilus yacare) (Daudin, 1802) were incubated at 30° C. After 20 days of incubation at 30° C, the remaining eggs were distributed in the following way: seven at 34° C, seven at 32° C, six at 30° C and six at 28° C. At 32° C and 34° C a 100% hatchability was obtained while at 30° C 83.3% and at 28° C 66.6%. After hatching, the animals were kept for 120 days at the same temperature of their final incubation. Weight (g) control and morphometric (cm) analysis were carried out. The statistic design was totally randomized and the data were treated by regression analysis showing a quadratic behavior ( p&lt;0.05). It was found that weight, total length (TTL), tail length (TL), snout- vent length (SVL), head length...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caiman; Development; Incubation; Temperature; Thermic variation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300012

Effect of plant growth regulators in the rooting of Pinus cuttings
Provedor de dados: 52
Autores: Henrique,Andréia; Campinhos,Eduardo Nogueira; Ono,Elizabeth Orika; Pinho,Sheila Zambello de.
This work evaluated the rooting of Pinus caribaea var. hondurensis Morelet cuttings under the action of different levels of plant growth regulators. The cuttings consisted of 4-6 cm long shoots of P. caribaea var. hondurensis Morelet with their basal needles removed. The basal part of the cuttings were treated for 2 seconds with the following treatments: 1- NAA 2000mg L-1; 2- NAA 4000mg L-1; 3- NAA 6000mg L-1; 4- NAA 2000mg L-1 + PBZ 100mg L-1; 5- NAA 4000mg L-1 + PBZ; 6- NAA 6000mg L-1 + PBZ; 7- IBA 2000mg L-1; 8- IBA 4000mg L-1; 9- IBA 6000mg L-1; 10-IBA 2000mg L-1 + PBZ; 11- IBA 4000mg L-1 + PBZ; 12- IBA 6000mg L-1 + PBZ; and a control. After receiving the treatment, the cuttings were planted in tubes containing 50% carbonized rice hulls and 50%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auxins; Paclobutrazol; Pinus caribaea.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300002

Tree species sprouting from root buds in a semideciduous forest affected by fires
Provedor de dados: 52
Autores: Rodrigues,Ricardo R.; Torres,Roseli B.; Matthes,Luis A. F.; Penha,Alessandra S.
Tree species which sprouted from root buds were identified in a 3.6ha area of a Semideciduous Seasonal Forest fragment in Campinas, São Paulo State (22° 55' S and 47° 05' W), affected by several fires. Connection between sprouts and main trunk root system was confirmed by digging and root exposure. Twenty seven tree species with root buds were identified, with a high proportion of Leguminosae species. The majority of identified species (45%) were characteristic of initial secondary succession stages. Distances between sprouts and main trunks varied from 0.6m (Guettarda viburnioides Cham. and Schltdl- Rubiaceae.) to 14.0m (Colubrina glandulosa Perkins- Rhamnaceae). These results lead discussions about natural and/or human-made disturbances influence on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disturbances; Forests; Fire; Root buds; Sprouting; Vegetative propagation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100017

Role of Glutaraldehyde in Imparting Stability to Immobilized β-Galactosidase Systems
Provedor de dados: 52
Autores: Satar,Rukhsana; Jafri,Mohammad Alam; Rasool,Mahmood; Ansari,Shakeel Ahmed.
ABSTRACT This review article highlights the role of glutaraldehyde as a matrix activator/stabilizer in imparting higher operational and thermal stability to β-galactosidase (βG) for biotechnological applications. Glutaraldehyde has been used extensively as a crosslinking agent as well as for functionalization of matrices to immobilize β-galactosidase. Immobilized β-galactosidase systems (IβGS) obtained as a result of glutaraldehyde treatment has been employed to hydrolyze whey and milk lactose in batch reactors, continuous packed-bed and fluidized bed reactors under various operational conditions. Moreover, these IβGS have also been utilized for the production of galactooligosaccharides in food, dairy and fermentation industries. It was observed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-galactosidase; Crosslinking; Glutaraldehyde; Immobilization; Stability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100447

Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
Provedor de dados: 52
Autores: Mall,Puspadhwaja; Mohanty,Bijaya Kumar; Patankar,Dhananjay Bhawanishankar; Mody,Rustom; Tunga,Rashbehari.
The influence of various physiochemical parameters on the growth of Lactococcus lactis sub sp. lactis MTCC 440 was studied at shake flask level for 20 h. Media optimization (MRS broth) was studied to achieve enhanced growth of the organism and also nisin production. Bioassay of nisin was done with agar diffusion method using Streptococcus agalactae NCIM 2401 as indicator strain. MRS broth (6%, w/v) with 0.15μg/ml of nisin supplemented with 0.5% (v/v) skimmed milk was found to be the best for nisin production as well as for growth of L lactis. The production of nisin was strongly influenced by the presence of skimmed milk and nisin in MRS broth. The production of nisin was affected by the physical parameters and maximum nisin production was at 30(0)C while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nisin; Lactococcus lactis; Streptococcus agalactae; Skimmed milk.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100025

Performance analysis of a trickle irrigation subunit installed in an apple orchard
Provedor de dados: 52
Autores: Soccol,Olívio José; Ullmann,Mario Nestor; Frizzone,José Antônio.
Studies were carried out to evaluate the "xique-xique" irrigation system of a trickle irrigated subunit. Initial results showed that the system's performance was average, any Emission Uniformity, Statistical Uniformity and Coefficient of Global Variation were 74.51, 77.69, and 22.31%, respectively. The efficiency parameters were below expectations, and values of Application Efficiency (Ae), Storage Efficiency (Se), Deep Percolation (Dp), Deficit Degree (DD) and Adequacy Degree (a) were 100, 47.83, 0, 52.17 and 0% respectively. By adopting new management procedures, it was possible to improve the system's efficiency, reaching values of 90.20, 91.68, 9.80, 8.32 and 52.87% for Ae, Se, Dp, DD and "a", respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irrigation; Efficiency; Uniformity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600017

Characterization and Metal Detoxification Potential of Moderately Thermophilic Bacillus cereus from Geothermal Springs of Himalaya
Provedor de dados: 52
Autores: Ghalib,Aslam Khan; Yasin,Muhammad; Faisal,Muhammad.
Two thermophilic Bacillus cereus strains (B. cereus-TA2 and B. cereus-TA4) used in the present study were isolated from the geothermal spring of Hunza valley, Gilgit, Pakistan. They showed the ability to withstand and grow at high temperature (85°C). Both these strains could resist multiple metals (copper, cadmium, mercury, manganese, zinc, arsenic, chromium and selenium). Strain B. cereus-TA4 reduced Cr (VI) at pH 5.0 to 9.0 but maximum reduction (83%) was observed at pH 7.0 after 48 h when initially supplied with 200 µg mL-1of K2CrO4. Lower initial concentrations such as 100 µg mL-1 supported higher reduction (90 to 95%) than that of high concentration such as 500 µg mL-1 (20 to 30%). Both the strains reduced nearly 70% of Se (IV) after 48 h of growth at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromium; Bioremediation; Selenium; Heavy metals.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400554

An outbreak of staphylococcal food poisoning in the Municipality of Passos, MG, Brazil
Provedor de dados: 52
Autores: Carmo,Luiz Simeão do; Dias,Ricardo Souza; Linardi,Valter Roberto; Sena,Maria José de; Santos,Deise Aparecida dos.
An outbreak of staphylococcal food poisoning involving 42 people who had eaten a meal at a restaurant in the Municipality of Passos, Minas Gerais, Brazil, is reported. Thirty-one of the individuals became ill with vomiting, diarrhea and dizziness within 30 minutes after eating the meal. The foods suspected were: chicken pancake, rice, beans, tomato sauce and mashed chick-peas. Large numbers (&gt; 2.0x10(8) CFU/g) of enterotoxigenic staphylococci were present in the chicken pancake. These strains produced enterotoxins A, B and D. Swabs from the nasal cavity and throat and from under the fingernails of food handlers were cultured for the detection of enterotoxigenic staphylococci carriers. Four out of five of them were healthy carriers of enterotoxin A, B, C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcal enterotoxin; Food poisoning; Staphylococci; Food handlers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400012

Botanical Knowledge in the Law Enforcement: a Preliminary Approach Towards its Potential Use in Brazilian Criminal Caseworks
Provedor de dados: 52
Autores: Manvailer,Vinicius; Leandro,Thales D.; Scremin-Dias,Edna.
ABSTRACT We surveyed Brazilian forensic institutes in order to provide a baseline for botanical knowledge used in forensic applications. Our approach provides a better understanding of the law enforcement dynamics as a whole, and establishes perspectives and guidelines for implementing this discipline in Brazil, enhancing the elucidative power of Brazilian forensic institutes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botany; Criminal Investigation; Criminalistics; Forensic Sciences; Plant Sciences.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100436

Contribuição ao levantamento fitossanitário do Estado do Paraná
Provedor de dados: 52
Autores: Vellozo,Lycio G. C.; Nowacki,M. J.; Vernalha,M. M.
A previous report of plants diseases, observed at the Division of Plant Biology of the Instituto de Biologia e Pesquisas Tecnológicas - Paraná - Brasil, during the years 1946-1950.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plaint diseases; State of Paraná; Phytosanitary survey.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500013

Studies on alkalophilic CGTase-producing bacteria and effect of starch on cyclodextrin-glycosyltransferase activity
Provedor de dados: 52
Autores: Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Nascimento,Aguinaldo José do.
The production of beta- and gamma -CD by CGTase from Bacillus firmus was studied, in regard to the effect of the source and concentration of starch on the yield of CD and CGTase activity. A cyclic activity of accumulation and consumption of beta -CD and gamma -CD occurred during the bacterial growth. CGTase was more active when citrate buffer, pH 5.5 was used. No differences were found for production of gamma -CD with the use of commercial starch flour when compared with corn starch (p&gt;0.05). The use of commercial starch flour resulted in decreased conversion of starch to beta-CD (all the 4 starch sources were statistically different, p&lt;0.05). The best results for beta -CD production were obtained with the use of corn starch. The specific activity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Starch.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100018

Demographic aspects of Chrysomya megacephala (Diptera, Calliphoridae) adults maintained under experimental conditions: reproductive rate estimates
Provedor de dados: 52
Autores: Carvalho,Marcelo Henrique de; Von Zuben,Claudio José.
The objective of this work was to evaluate some aspects of the populational ecology of Chrysomya megacephala, analyzing demographic aspects of adults kept under experimental conditions. Cages of C. megacephala adults were prepared with four different larval densities (100, 200, 400 and 800). For each cage, two tables were made: one with demographic parameters for the life expectancy estimate at the initial age (e0), and another with the reproductive rate and average reproduction age estimates. Populational parameters such as the intrinsic growth rate (r) and the finite growth rate (lambda) were calculated as well.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chrysomya megacephala; Calliphoridae; Demographic aspects; Reproductive parameters; Cohort's mean reproductive age.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400014

The mangrove as a temporary habitat for fish: the Eucinostomus Species at Guaratuba Bay, Brazil (25º 52'S;48º 39'W)
Provedor de dados: 52
Autores: Chaves,Paulo de Tarso C.; Otto,Gislaine.
Several coastal fish use the estuarine habitat during a part of their life cycle. These sites are considered good for the reproductive activity, as well as for the growth of larvae and juveniles. Concerning the Gerreidae, however, many studies reveal that most species leave the estuaries to reproduce at sea. At Guaratuba Bay, southern Brazil, this family is represented by three genera and five species, which make an important fraction of the local assemblage. The present study investigated the populational structure and breeding habits of three Eucinostomus species, in order to know what relationship exists between them and the mangrove. It was found that the Guaratuba mangrove represents a transitory habitat for the life cycle of the Eucinostomus species....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Gerreidae; Estuaries; Mangrove.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100009

Development, survival and reproduction of Podisus nigrispinus (Dallas, 1851) (Heteroptera: Pentatomidae) with salt and amino acids solutions supplementary diet
Provedor de dados: 52
Autores: Freitas,Simone Patrícia Carneiro; Evangelista Júnior,Walter Santos; Zanuncio,José Cola; Serrão,José Eduardo.
This study presents the effect of a supplementary diet with amino acids and sodium chloride solutions in addition to prey on the development, survival and reproduction of the predator Podisus nigrispinus (Heteroptera, Pentatomidae). Both solutions showed deleterious effects on nymph survival, adult weight, female longevity, number of egg masses, eggs per female, eggs per egg mass and nymphs per female besides egg viability of P. nigrispinus when compared with diet with water and prey. When compared with plant supplements in the diet the use of amino acids and salt solutions for mass rearing of P. nigrispinus was inferior.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrition; Zoophytophagous; Insecta; Biological control.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400013

Antioxidant Activities of Chicken Egg White Hydrolysates Obtained by New Purified Protease of Aspergillus avenaceus URM 6706
Provedor de dados: 52
Autores: Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Oliveira,João Tiago Correia; Medeiros,Erika Valente; Souza-Motta,Cristina Maria de; Moreira,Keila Aparecida.
Abstract Protein hydrolysates originating from egg white have already been reported to be bioactive and, among their biological activities, possess the antioxidant property that protects the body from early ageing and diseases linked to oxidation. Therefore, the objective of this work was to evaluate the antioxidant activity of hydrolysates obtained by the hydrolysis of egg white from hen poultry. The protease produced by Aspergillus avenaceus URM 6706 was purified and subsequently applied to hydrolysate the egg white, and the degree of hydrolysis was verified during the protease exposure time (4-24 h). The hydrolysis was intensified over time of exposure to the protease. It was possible to detect the antioxidant activities of eliminating the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus; Purification; ABTS; +; DPPH; Chelators.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100508

Main Stem and Tiller Contribution to Wheat Cultivars Yield Under Different Irrigation Regimes
Provedor de dados: 52
Autores: Destro,Deonisio; Miglioranza,Édison; Arias,Carlos Alberto Arrabal; Vendrame,Jefferson Marcos; Almeida,José Carlos Vieira de.
The present study was carried out to determine the contribution of main stems and tillers to the total yield of two wheat cultivars (Triticum aestivum L.), Cocoraque and BH-1146, under two water treatments: a) normal field conditions, and; b) irrigation, in Londrina, PR, Brazil. The experiment was an eight replication randomized complete block design with treatments arranged in split-plots. Data on total grain yield, main stem grain yield, tillers grain yield, the ratio between tiller and total grain yield, yield components and other 17 traits were collected. The cultivar BH-1146 had a higher total grain yield in relation to Cocoraque under normal field conditions; i, e., under water stress. Main stem grain yield responded positively and significantly to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Harvest index; Growth period; Hectolitric weight; Poaceae; Water stress.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400001

Bacteriocins: molecules of fundamental impact on the microbial ecology and potential food biopreservatives
Provedor de dados: 52
Autores: Souza,Evandro Leite de; Silva,Clemilson Antonio da; Sousa,Cristina Paiva de.
Bacteriocins are proteic molecules synthesized for various lineages of Gram-positive and Gram-negative bacteria when exposed to stressful conditions. Bacteriocins have been characterized as molecules of high antimicrobial property even at low concentrations, provoking the microbial survival inhibition by antibiosis. These substances have their synthesis mediated for genetic mechanisms and develop their lethal action on the microbial cell by multiples mechanisms that can act of isolated or concomitant way culminating with microbial cell killing. This molecules class presents characteristic of stability to heat, low pH, refrigeration and freezing, and resistance to weak organics solvents, salts and enzymes. On the other hand, they are very sensitive to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriocins; Antimicrobials; Food Industry; Biopreservation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500008

Correlation between heavy metal ions (copper, zinc, lead) concentrations and root length of Allium cepa L. in polluted river water
Provedor de dados: 52
Autores: Palacio,Soraya Moreno; Espinoza-Quiñones,Fernando Rodolfo; Galante,Raquel Manozzo; Zenatti,Dilcemara Cristina; Seolatto,Araceli Aparecida; Lorenz,Evandro Kleber; Zacarkim,Carlos Eduardo; Rossi,Nickeli; Rizzutto,Márcia de Almeida; Tabacniks,Manfredo Harri.
The present work was performed using the common onion (Allium cepa L.) as a bioindicator of toxicity of heavy metals in river water. The test waters were collected at two sampling sites: at the beginning and the end of the Toledo River. The bulbs of A. cepa L. were grown in test water with nine concentration levels of copper, zinc and lead from 0.1 to 50 ppm. In the laboratory, the influence of these test liquids on the root growth was examined during five days. For test liquids containing below 0.03-ppm dissolved Cu the root growth was reduced by 40% However, the same reduction occurred for 1-ppm dissolved Zn. For dissolved Pb, results reveal toxicity above 0.1 and 0.6 ppm at the beginning and the end of the Toledo river water, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute toxicity; Bioassay; Copper; Zinc; Lead; Allium cepa L.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400024

In silico molecular modeling and docking studies on the leishmanial tryparedoxin peroxidase
Provedor de dados: 52
Autores: Mutlu,Ozal.
Leishmaniasis is one of the most common form of neglected parasitic disease that affects about 350 million people worldwide. Leishmanias have a trypanothione mediated hydroperoxide metabolism to eliminate endogenous or exogenous oxidative agents. Both of 2-Cys peroxiredoxin (Prx) and glutathione peroxidase type tryparedoxin peroxidase (Px) are the terminal enzymes in the trypanothione dependent detoxification system. Therefore absence of trypanothione redox system in mammals and the sensitivity of trypanosomatids against oxidative stress, enzymes of this pathway are drug targets candidates. In this study, 3D structure of tryparedoxin peroxidase (2-Cys peroxiredoxin type) from Leishmania donovani (LdTXNPx) was described by homology modeling method based on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homology modeling; Docking; Leishmania donovani; Tryparedoxin peroxidase; Hydroperoxide metabolism.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200013

Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
Provedor de dados: 52
Autores: Bertassoli,Bruno Machado; Costa,Emanuela Silva; Sousa,Cristiane Aparecida; Albergaria,Juliano Douglas Silva; Maltos,Kátia L. Melo; Goes,Alfredo Miranda; Matins,Thais Maria da Mata; Silva,Gerluza Aparecida Borges; Jorge,Erika Cristina.
ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Dental pulp; Murine; Phenotypic characterization; Cellular differentiation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335

Genetic distance and its association with heterosis in cacao
Provedor de dados: 52
Autores: Dias,Luiz Antônio dos Santos; Marita,Jane; Cruz,Cosme Damião; Barros,Everaldo Gonçalves de; Salomão,Tânia Maria Fernandes.
The efficiency of cacao breeding program can be increased by choosing superior crosses to be made between divergent clones. We assessed the genetic distance among five clones with RAPD data (genetic distance - GD) and with yield component data (Mahalanobis distance - MD). The clones were evaluated in a diallel, during five years, for five yield components. A total of 130 RAPD bands were scored. GD and MD were used to determine the correlation between genetic distances among clones and the performance of their hybrids. The correlation between GD and MD was 0.67 (P=0.03). Both distances were related to heterotic performance of hybrids for wet seed weight/plant and wet seed weight/fruit. The average hybrid performance for the same two yield components was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic distances; Heterosis; Performance data; RAPD data; Theobroma cacao L.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300005

Contribution of the IAM Pathway to IAA Pool in Developing Rice Grains
Provedor de dados: 52
Autores: Abu-Zaitoon,Yousef; Aladaileh,Saleem; Tawaha,Abdel Rahman Al.
A possible role for the indole-3-acetamide (IAM) pathway in the indole-3-acetic acid (IAA) production was investigated in developing rice grains. IAM-hydrolase proposed to convert IAM to IAA primarily through the identification of IAM and IAM-hydrolase activity in some plant species. Expression profiles of the two putative rice IAM-hydrolase genes, OsAMI1&amp;2, were compared to the previously quantified IAA level. The abrupt increase in IAA level between 4 and 7 days after anthesis (DAF) was not found to correlate with changes in the expression of OsAMI1 or OsAMID2 suggesting that the IAM pathway may not contribute significantly to IAA pool in rice grains. Production of a biological compound other than IAA may explain the high activity of OsAMI1&amp;2 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indole-3-acetamide IAM-hydrolase Auxin; Indole-3-acetic acid Tryptophan.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100507

Erratum: Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100903

Factors affecting herbivory of Thrips palmi (Thysanoptera: Thripidae) and Aphis gossypii (Homoptera: Aphididae) on the eggplant (Solanum melongena)
Provedor de dados: 52
Autores: Leite,Germano Leão Demolin; Picanço,Marcelo; Zanuncio,José Cola; Ecole,Carvalho Carlos.
The objective of this study was to evaluate effects of total rainfall, mean temperature, natural enemies, chemical composition of leaves, levels of nitrogen and potassium on leaves and density of leaf trichomes on attack intensity of Thrips palmi Karny (Thysanoptera: Thripidae) and Aphis gossypii (Glover) (Homoptera: Aphididae) on plantations of the eggplant (Solanum melongena) in two regions of Minas Gerais, Brazil. Higher numbers of A. gossypii/leaf and T. palmi/leaf were observed in plantations of this eggplant in the Municipalities of Viçosa and Guidoval, respectively. Guidoval had a rainy and hotter weather than Viçosa. T. palmi was almost positivelly correlated with rainfall (r= 0.49, P= 0.0538) while A. gossypii seemed to be more affected by mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aphis gossypii; Natural enemies; Plant nutrition; Solanum melongena; Thrips palmi.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400002

Dynamics of ethanol production from deproteinized whey by Kluyveromyces marxianus: An analysis about buffering capacity,thermal and nitrogen tolerance
Provedor de dados: 52
Autores: Moreira,Nathalia Lima; Santos,Leandro Freire dos; Soccol,Carlos Ricardo; Suguimoto,Hélio Hiroshi.
The production of value-added products could be a valuable option for cheese wastewater management. However, this kind of study cannot just focus alone on getting the final product. This also necessitates studies on the dynamics of bioprocesses. With these as background, the present investigation aimed at evaluating the buffering capacity of deproteinized whey and effect of temperature and nitrogen source on ethanol yields from it. The batch fermentation conditions used to evaluate ethanol production were temperatures 30, 35, 40°C and pH 4.5, 5.0, 5.5, 6.0. To study the influence of nitrogen source on ethanol yield, a design matrix was applied using yeast extract and (NH4)2SO4.The final pH was analyzed to evaluate the buffering capacity. The results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol fermentation; Dairy byproduct; Whey management.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300454

Combined pig slurry and mineral fertilization for corn cultivation
Provedor de dados: 52
Autores: Prior,Maritane; Sampaio,Silvio César; Nóbrega,Lúcia Helena Pereira; Opazo,Miguel Angel Uribe; Dieter,Jonhatan; Pegoraro,Thaisa.
The objective of this work was to evaluate the environmental effects of the use of pig slurry to irrigate the corn crop grown in a typical Red Distroferric Latosol and in leachate composition. Twenty four lysimeters, filled with soil, in a protected environment, received five doses of pig slurry (0; 112.5; 225; 337.5 and 450 m³ ha-1 per growing cycle) combined with two mineral fertilization doses (50 and 75% of the recommended dose - 80 kg ha-1of nitrogen). Corn height and yield were evaluated, as well as nutrients in the soil and in leachate. From the results obtained in the first year of experiment, it was concluded that the concentrations of nitrate increased by increase of pig slurry application, and thus, it would be necessary to develop further...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrate leaching; Nutrients cycling; Zea mays L.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200020

Mathematical modeling of convective air drying of quinoa-supplemented feed for laboratory rats
Provedor de dados: 52
Autores: Vega-Gálvez,Antonio; Dagnino-Subiabre,Alexies; Terreros,Gonzalo; López,Jessica; Miranda,Margarita; Di Scala,Karina.
Drying kinetics of quinoa-supplemented feed for laboratory rats during processing at 50, 60, 70, 80 and 90ºC was studied and modeled in this work. Desorption isotherm was obtained at 60ºC giving a monolayer moisture content of 0.04 g water/g d.m. The experimental drying curves showed that drying process took place only in the falling rate period. Several thin-layer drying equations available in the literature were evaluated based on determination coefficient (r²), sum squared errors (SSE) and Chi-square (χ2) statisticals. In comparison to the experimental moisture values, the values estimated with the Logarithmic model gave the best fit quality (r² &amp;gt;0.994, SSE &lt; 0.00015 and χ2 &lt; 0.00018), showing this equation could predict very accurately the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat feed; Quinoa; Drying kinetics; Modeling; Statistical test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100021

Variação nos hábitos alimentares da mosca de estábulos Stomoxys calcitrans L
Provedor de dados: 52
Autores: Gonçalves,Neuza Maria Ferraz de Mello; Veiga,L. A. S.
During the course of a study on the behaviour, of Stomoxys calcitrans a research on the feeding habits of this fly was carried out. A total of 27l4 flies were caught in three poultry ranches from Araucária, Paraná. The source of the flie's sucked blood has been established by precipitin test. . The results showed that although almost all the flies usually feed mammal blood, some of them gave positive precipitin test for birds blood. Although all flies were caught on poultry ranches, none had feed on chickens but 2,7% of the total had feed on ducks, 0.7% on geese and 0.37% on turkeys.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stomoxys calcitrans; Mosca de estábulos; Teste de precipitina; Alimentação em aves.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300011

Growth and Differentiation Factor-9 Supplementation Affects Viability and Morphology of Preantral Follicles in Equine Ovarian Fragments During Short-term in vitro Culture
Provedor de dados: 52
Autores: Marino,Polyana Carolina; Bizarro-Silva,Camila; Búfalo,Isabela; Rosa,Camila Oliveira; Gonçalves,Gustavo Romero; Lisboa,Livia Aires; Seneda,Marcelo Marcondes.
Abstract This study aimed to evaluate different concentrations of growth and differentiation factor-9 (GDF-9) on the development and maintenance of equine preantral follicle morphology during short-term in vitro culture. Ovaries (n=5) from five mares were collected from a local slaughterhouse and transported to the laboratory, where nine fragments (5x5x1mm) were procured from each ovary. One fragment from each was immediately fixed and submitted for histological analysis (control group; D0). The other eight fragments were cultured in situ for two (D2) or six (D6) days in MEM+ or MEM+ supplemented with GDF-9 at different concentrations (i.e., 50, 100 and 200 ng/mL the GDF-9). After culturing with different concentrations of GDF-9 for 2 or 6 days, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Assisted Reproduction; GDF-9; Mare.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100515

A comparative evaluation of antibacterial potential of some plants used in indian traditional medicine for the treatment of microbial infections
Provedor de dados: 52
Autores: Chattopadhyay,Rabi Ranjan; Bhattacharyya,Subir Kumar; Medda,Chiranjib; Chanda,Sunanda; Bag,Anwesa.
A comparative in vitro antibacterial potential of extracts (aqueous and ethanol) of five important medicinal plants (Aegle marmelos, Azadirachta indica, Terminalia chebula, Mangifera indica and Ocimum sanctum) were investigated using microbial growth inhibition assays against the common human pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli) of clinical origin. All the plant materials showed varying degrees of strain specific inhibitory action and ethanol extract of the plant materials showed higher antibacterial activity than their aqueous counterparts. Besides, T. chebula and A. marmelos had the strongest antibacterial activity out of which, T. chebula possessed a wider spectrum and a superior antibacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenic bacteria; Medicinal plants; Antibacterial potential; Comparative study.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500009

Fat body morphology of Eriopis connexa (coleoptera, coccinelidae) in function of two alimentary sources
Provedor de dados: 52
Autores: Sarmento,Renato de Almeida; Oliveira,Hamilton Gomes de; Holtz,Anderson Mathias; Silva,Solange Marques da; Serrão,José Eduardo; Pallini,Ângelo.
This work investigated the influence of two alimentary sources: aphids and mites, in the morphology of the fat body of E. connexa. Results showed that fat body cells of individuals fed with aphids presented areas more than three folds higher (511 ± 98,7µm²) than those of beetles fed with mites (162 ± 34,9µm²). Histochemical tests showed that nutrient amount stored in fat body was higher in lady-beetles fed on aphids.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alimentary source; Aphids; Eriopis connexa; Fat body; Mites.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300011

Optimization Medium Composition for Vitamin K2 by Flavobacterium sp. using Response Surface Methodology and Addition of Arachis hypogaea
Provedor de dados: 52
Autores: Tan,Mu; Liu,Hui; Li,Zhemin; Sun,Xiaowen; Zheng,Zhiming; Zhao,Genhai.
ABSTRACT The purpose of this research was to enhance the production of vitamin K2 by fermentation optimization and Arachis hypogaea supplementation in Flavobacterium sp. mutant SP-L-01. Optimized culture condition were as follows: 6-days shake-flask culture at 37oC with initial pH value 7.0 ± 0.2, shaking speed in 120 r/min and medium volume of 30 mL with 2% inoculums. After optimization of fermentation medium by response surface methodology (RSM), optimized medium were maltose 23.8 g/l, glucose 9.69 g/l, beef extract 15 g/l, K2HPO4 4.5 g/l，NaCl 3.0 g/l and MgSO4·7H2O 0.3 g/l. Production of vitamin K2 after optimization reached to 10.97 mg/l, which is 79.25% higher than that before optimization (6.12 mg/l). 3 mg/mL of arachis hypogaea was added into the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis hypogaea; D-(+)-catechin; Response surface methodology; Vitamin K2.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100501

Cytogenetics and biogeography: considerations about the natural origin of Hoplias malabaricus (Characiformes, Erythrinidae) on the Iguaçu river
Provedor de dados: 52
Autores: Vicari,Marcelo Ricardo; Pazza,Rubens; Artoni,Roberto Ferreira; Margarido,Vladimir Pavan; Bertollo,Luiz Antonio Carlos.
Hoplias malabaricus (trahyra) is a widespread fish species over the Neotropical region with diversified inter-populational karyotypes (cytotypes), which may correspond to a species complex. Despite the wide distribution in the South American basins, some authors have questioned its natural origin in the Iguaçu river, an important Brazilian river basin which is characterized by several endemic fish species. We have analyzed the karyotype of H. malabaricus from different collection sites of this river, by conventional and banding methods. Our results, in addition to our previous data concerning geographic distribution of the cytotypes, contribute to better understand the origin of H. malabaricus on the Iguaçu river, reinforcing the proposition that it is a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome evolution; Karyotypes distribution; Endemism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300015

Susceptibilities to carbapenems and presence of cphA gene on food-borne Aeromonas
Provedor de dados: 52
Autores: Martín Talavera,Bibiana María; Benassi,Fernando Olivio; von Specht,Martha Helena; Quiroga,Marina Inés; García,Myriam Alicia; Pucciarelli,Amanda Beatríz; Zubreski,Emilce; Laczeski,Margarita Esther; Gutkind,Gabriel.
The purpose of this study was to determine the susceptibilities of food-borne Aeromonas to carbapenems, as well as to investigate the presence of a metallo carbapenemase-encoding gene, named cphA. Minimum Inhibitory Concentration (MIC) was determined following NCCLS standards. All the tested microorganisms were susceptible to imipenem, meropenem and biapenem. However, a strong inoculum size effect on carbapenem MICs was observed for most of the strains. Six strains, out of seven, showed the presence of metallo--beta-lactamases but cphA gene was detected in only two strains of A. veronii bv. sobria.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metallocarbapenemase; CphA gene; Food borne Aeromonas.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500019

Alternative Control of Plant Pathogen Fungi Through Ethanolic Extracts of Avocado Seeds (Persea Americana Mill.)
Provedor de dados: 52
Autores: Fagundes,Miriã Cristina Pereira; Oliveira,Adelson Francisco de; Carvalho,Vicente Luiz de; Ramos,José Darlan; Santos,Verônica Andrade dos; Rufini,José Carlos Moraes.
ABSTRACT The aim of this study was to evaluate the antifungal action of ethanolic extracts of avocado seeds, by conducting two experiments in the laboratory of plant pathology of EPAMIG SUL/Lavras, MG, Brazil, in a completely randomized design with three replicates. The first consisted of the following treatments ('Breda' 3%, 'Breda' 2%, 'Margarida' 3%, 'Margarida' 2%, Control, and Ethanol) testing its inhibitory effect on two fungal species (Colletotrichum gloeosporioides and Monilinia fructicola) and the second consisted of different concentrations of extracts (0%, 0.25%, 0.50%, 0.75%, 1.0%, 1.5%, and 2.0%) on the fungus F fructicola. The evaluations were performed at three times (7, 14, and 21 days of incubation) by measurements of the mycelial diameter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal activity; Colletotrichum gloeosporioides; Monilinia fructicola; Postharvest.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100204

A Histopathological, Immunohistochemical and Biochemical Investigation on the in vitro Antioxidant, Myeloprotective, Hematoprotective and Hepatoprotective Effects of Hypericum triquetrifolium Seed Extract Against Cyclophosphamide-Induced Toxicity
Provedor de dados: 52
Autores: Yildiz,Songul Cetik; Keskin,Cumali; Sahintürk,Varol; Ayhanci,Adnan.
Abstract  The aim of this study was to investigate in vitro antioxidant properties and in vivo protective effects of the methanol extract of the Hypericum triquetrifolium Turra (HT) seed against acute hepatotoxicity, myelotoxicity and hematotoxicity in rats induced by cyclophosphamide (CP). In order to investigate in vivo protective effects of the HT extract on rat tissues, the rats were divided into nine groups. The toxic effects of CP and the protective effects of HT extract on nucleated cells that are produced by bone marrow, serum alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and oxidative stress index (OSI) levels were investigated biochemically. Additionally, liver tissue samples were examined for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclophosphamide; Hematoprotective; Hepatoprotective; Hypericum triquetrifolium; Myeloprotective; Pharmacological activity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100303

Stem cells and respiratory diseases
Provedor de dados: 52
Autores: Abreu,Soraia Carvalho; Maron-Gutierrez,Tatiana; Garcia,Cristiane Sousa Nascimento Baez; Morales,Marcelo Marcos; Rocco,Patricia Rieken Macedo.
Stem cells have a multitude of clinical implications in the lung. This article is a critical review that includes clinical and experimental studies of MedLine and SciElo database in the last 10 years, where we highlight the effects of stem cell therapy in acute respiratory distress syndrome or more chronic disorders such as lung fibrosis and emphysema. Although, many studies have shown the beneficial effects of stem cells in lung development, repair and remodeling; some important questions need to be answered to better understand the mechanisms that control cell division and differentiation, therefore enabling the use of cell therapy in human respiratory diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Fibrosis; Repair; Acute respiratory distress syndrome; Asthma; Emphysema.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700005

Biodegradation of 2,4 dichlorophenol by Pleurotus ostreatus DSM 1833
Provedor de dados: 52
Autores: Silva,Heloisa Helena Batista da; Schneider,Andréa Lima dos Santos; Wisbeck,Elisabeth; Furlan,Sandra Aparecida.
This work aimed to investigate the capacity of Pleurotus ostreatus DSM 1833 to degrade 2,4-dichlorophenol, important pollutant found in the wastewaters of the paper and cellulose industry. Using a factorial design 2², the concentrations of glucose and 2,4-dichlorophenol varied between 0 and 10g.L-1 and 5 and 30mg.L-1, respectively. The best global biodegradation rate was obtained using 30 mg.L-1 of 2,4- dichlorophenol in the absence of glucose. This culture medium was used for scaling up the process, resulting in a global biodegradation rate of 0.47mg.L-1.h-1. A comparative test between an inoculated medium and an abiotic control demonstrated that 54.1% of 2,4- dichlorophenol degradation could be attributed to the presence of P. ostreatus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation 2; 4-dichlorophenol paper and cellulose industry Pleurotus ostreatus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600028

Effect of temperature on two-phase anaerobic reactors treating slaughterhouse wastewater
Provedor de dados: 52
Autores: Beux,Simone; Nunes,Ezequiel; Barana,Ana Cláudia.
The effectiveness of the anaerobic treatment of effluent from a swine and bovine slaughterhouse was assessed in two sets of two-phase anaerobic digesters, operated with or without temperature control. Set A, consisting of an acidogenic reactor with recirculation and an upflow biological filter as the methanogenic phase, was operated at room temperature, while set B, consisting of an acidogenic reactor without recirculation and an upflow biological filter as the methanogenic phase, was maintained at 32°C. The methanogenic reactors showed COD (Chemical Demand of Oxygen) removal above 60% for HRT (Hydraulic Retention Time) values of 20, 15, 10, 8, 6, 4, and 2 days. When the HRT value in those reactors was changed to 1 day, the COD percentage removal decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Effluent; Slaughterhouse; Treatment; Anaerobic; Two-phase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700017

Study of phycocyanin production from Spirulina platensis under different light spectra
Provedor de dados: 52
Autores: Walter,Alfredo; Carvalho,Júlio Cesar de; Soccol,Vanete Thomaz; Faria,Ana Bárbara Bisinella de; Ghiggi,Vanessa; Soccol,Carlos Ricardo.
The aim of this work was to investigate the production of phycocyanin by Spirulina platensis under different spectra of light. The dependent variables evaluated were the amount of phycocyanin obtained and its purity, demonstrating that there might be a restructuring of phycobilisomes, especially when the culture was subjected to red light, which increased the purity level up to 33% with a reduction of 16% in phycocyanin content, but with higher photosynthetic efficiency compared to natural light.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spirulina; Phycocyanin; Phycobilisome; Pigment; Spectrum; Chromatic acclimation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400005

Origins of Bipedalism
Provedor de dados: 52
Autores: Ko,Kwang Hyun.
ABSTRACT This article aimed to review various theories of bipedalism and provide a holistic answer to human evolution. There have been two questions regarding bipedalism: i) why were the earliest hominins partially bipedal?, and ii) why did hominins become increasingly bipedal over the time and replaced their less bipedal ancestors? To answer these questions, the prominent theories in the field, such as the savanna-based theory, the postural feeding hypotheses, and the provisioning model, are collectively examined. Because biological evolution is not a simple causation; there may be multiple answers to the evolution of bipedalism. The postural feeding hypothesis (reaching for food/balancing) provides an explanation for the partial bipedalism of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Locomotion; Provisioning model; Bipedalism; Chimps; Savannah; Origins of Bipedalism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600929

Study of the Performance Reduction Due to the Dirt Effect in the Photovoltaic Systems of UTFPR - Curitiba
Provedor de dados: 52
Autores: Tonolo,Édwin Augusto; Mariano,Juliana D’Angela; Neves,Carlos Eduardo Teixeira; Silva,Alynne Penha de Mendonça; Urbanetz Junior,Jair.
ABSTRACT Photovoltaic systems have been consolidated in the global energy scenario as an option of low environmental impact energy generation, high reliability and great applicability in urban centers, acting like energy generators near the point of consumption. The Federal University of Technology of Paraná (UTFPR), with the proposal of testing the performance of grid-connected photovoltaic systems (On Grid PV Systems) and help its entry into the Brazilian energy matrix, implemented this technology in two of its buildings: Green Office (GO) And Neoville. This paper analyzed the effects of dust on the Photovoltaic Systems performance based on daily energy. The analysis was carried out from the solar irradiance data from the places where the panels are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic system; Dust; Power generation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200220

The cytogenetic effects of black tea and green tea on cultured human lymphocytes
Provedor de dados: 52
Autores: Eroğlu,Halil Erhan.
In this study, the cytogenetic effects of black tea and green tea were determined in cultured peripheral blood lymphocytes. Results showed that black tea and green tea induced the mitotic and replication indexes and decreased micronuclei. But these data were not statistically significant for green tea. The effects of black tea on the micronucleus formation and mitotic index were statistically significant. The decrease in micronucleus counts indicated that black tea and green tea had considerable anticlastogenic and antigenotoxic effects as observed in vitro in human lymphocytes. Thus, it could be concluded that tea polyphenols protected the normal cells from genotoxic or carcinogenic agents, which indicated the therapeutic and antioxidative role of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tea; Catechins; Micronucleus; Mitotic index; Replication index.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600011

Epibiosis Reduction on Productivity in a Mussel Culture of Perna perna (Linné, 1758)
Provedor de dados: 52
Autores: Metri,Rafael; Rocha,Rosana Moreira da; Marenzi,Adriano.
Cultivated mussels (Perna perna) were studied to test for the effects of cleaning on their growth. These effects were examined by experimentally cleaning mussels and by changing mussel density. Treatment was performed twice, at two and four months after immerging the ropes. Two months after the first treatment and three months after the second treatment, mussels were harvested and measured (weight, length, width and thickness). Analysis of variance showed that none of the treatments resulted in increased growth of the mussels, nor did increased density result in decreased growth when compared with the control. It was concluded that it was not necessary to clean the shells to increase harvest.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mussels culture; Perna perna; Epibiosis; Cleaning management.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300011

Genetic variability of pre and post-fragmentation cohorts of Aspidosperma polyneuron Muell. Arg. (Apocynaceae)
Provedor de dados: 52
Autores: Torezan,José Marcelo Domingues; Souza,Rogério Fernandes de; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Camargo,Elvis Hassmann; Vanzela,André Luís Laforga.
RAPD was used to access the genetic variability in Aspisdosperma polyneuron, a long-lived, late-reproducing tropical tree, and highly important for the Atlantic Forest. RAPD profiles from adults (pre-fragmentation, &gt;300 years old) and seedlings (post-fragmentation, &lt;&lt;50 years old) were analyzed. Results showed a decrease of genetic polymorphism of post-fragmentation cohorts in small fragments and higher genetic diversity within population. The genetic diversity distribution suggested the establishment of fragments as protected reserves, and the transference of seedlings among fragments for conservation of A. polyneuron.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspidosperma; DNA markers; Forest fragmentation; Genetic variability; Peroba-rosa; RAPD.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200002

Effect of tripanosomicide benznidazole (Rochagan®) on the biodistribution of sodium pertechnetate (Na99mTcO4) in Wistar rats
Provedor de dados: 52
Autores: Barbosa,Vanessa Santos de Arruda; Holanda,Maria de Carvalho Xavier; Silva,Roseane Pereira da; Oliveira,Daniel Pereira de; Silva Júnior,Maurício Ferreira da; Oliveira,Elias Herculano de; Spyrides,Maria Helena Constantino; Medeiros,Aldo Cunha.
Benznidazole, a drug with specific anti-Trypanosoma cruzi activity, is used in the treatment of Chagas' disease. The radiopharmaceutical sodium pertechnetate (Na99mTcO4) is used to obtain diagnostic images of the stomach, thyroid, parathyroids, salivary glands, brain and in the study of esophageal reflux and blood flow. This study aimed at evaluating in vivo the influence of benznidazole treatment on the sodium pertechnetate biodistribution in Wistar rats. The percentage of radioactivity per gram (%ATI/g) of various organs (brain, heart, esophagus, stomach, small intestine, large intestine, spleen, liver, muscle and blood) was determined. Comparing the treated rats with the controls, we observed that sodium pertechnetate biodistribution did not change when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiparasite; Radiopharmaceutical; Technetium-99m; Benznidazole; Biodistribution; Trypanosoma cruzi.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700028

Evaluation of the effects of pressure gradients on four Brazilian freshwater fish species
Provedor de dados: 52
Autores: Pompeu,Paulo dos Santos; Horta,Luiz Felipe Mascarenhas; Martinez,Carlos Barreira.
This work aimed to experimentally evaluate the behavior of Brazilian freshwater fish species when submitted to a gradual increase in pressure, as well as sudden decompression's effects simulating the passage through a hydroelectric turbine. Four species from the São Francisco river basin were tested: Astyanax bimaculatus, Hypostomus sp., Leporinus reinhardti and Prochilodus costatus. For all of them mortality rates due to decompression were extremely low. However, the symptoms related to decompression, such as bulged eyes and hemorrhage, were not observed only in Hypostomus sp., and were more frequent the larger the pressure values were, considering the values from which decompression was performed. All these symptoms decreased significantly after 24 h of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Downstream fish passage; Dams; Experimental apparatus; Fish mortality; Pressure effects on fish.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100015

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology
Provedor de dados: 52
Autores: Turkez,Hasan; Sonmez,Erdal; Turkez,Ozlem; Mokhtar,Yousef Ibrahem; Stefano,Antonio Di; Turgut,Guven.
Tungsten (VI) oxide (WO3) nanoparticles (NPs) are used for many industrial purposes in everyday life. However, their effects on human health have not been sufficiently evaluated. Therefore, the present study was designed to investigate the toxicity potentials of various concentrations (0 to 1000 ppm) of WO3NPs (&lt;100 nm particle size) in cultured primary rat hepatocytes. The results of cell viability assay showed that the higher concentrations of dispersed WO3 NPs (300, 500 and 1000 ppm) caused significant (p&lt;0.05) decreases of cell viability. Also, dose dependent negative alterations were observed in oxidative status and antioxidant capacity levels after the application of WO3 in cultured rat primary hepatocytes. The results of genotoxicity tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tungsten oxide; Nanotoxicity; Genotoxicity; Cytotoxicity; Hepatocytes; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400532

Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor
Provedor de dados: 52
Autores: Komarkova,Eliska; Paca,Jan; Klapkova,Eva; Stiborova,Marie; Soccol,Carlos R; Sobotka,Miroslav.
Candida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be formed. An accumulation of these products inhibited the cell growth under aerobic conditions. Another impact was a decrease of the phenol hydroxylase activity as the key enzyme of the phenol degradation pathway at the end of the cell respiration activity. This decrease is linked with the above mentioned product inhibition. The cell death studied by fluorescent probe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenol; Biodegradation; Candida tropicalis; Phenol hydroxylase; Starvation stress; Viability.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007

A methodological proposal for quantifying environmental compensation through the spatial analysis of vulnerability indicators
Provedor de dados: 52
Autores: Torresan,Fabio Enrique; Lorandi,Reinaldo.
The aim of this work was to propose a methodology for quantifying the environmental compensation through the spatial analysis of vulnerability indicators. A case study was applied for the analysis of sand extraction enterprises, in the region of Descalvado and Analândia, inland of São Paulo State, Brazil. Environmental vulnerability scores were attributed for the indicators related to erosion, hydrological resources and biodiversity loss. This methodological proposal allowed analyzing the local alternatives of certain enterprise with the objective of reducing impacts and at the same time reducing the costs of environmental compensation. The application of the methodology significantly reduced the subjectivity degree usually associated to the most of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental compensation; Environmental impact assessment; GIS; Landscape ecology; Sand mining.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300026

Effects of wastewater from a cassava industry on soil chemistry and crop yield of lopsided oats (Avena strigosa Schreb.)
Provedor de dados: 52
Autores: Cabral,Juarez Rogério; Freitas,Paulo Sérgio Lourenço; Bertonha,Altair; Muniz,Antônio Saraiva.
This experiment was carried out in the Arenito soil of the Cidade Gaúcha county, Northwest Paraná in Brazil, in which 0, 150, 300, 450 and 600 m³ ha-1 of wastewater were applied onto soil and the crop yield and soil chemistry were investigated for lopsided oat (Avena strigosa Schreb.) plants. The crop yield from the control was 2818 kg ha-1 contrasting 3629 kg ha-1 when 300 m³ ha-1 of cassava wastewater were applied, and potassium, after haversting, was the only nutrient found in a higher concentration in the soil profile.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Effluents; Wastewaters; Irrigation; Fertirrigation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100003

Energy Management in Industry: An Enterprise Engineering Approach
Provedor de dados: 52
Autores: Silva,Vilson Roiz Gonçalves Rebelo da; Loures,Eduardo de Freitas Rocha; Lima,Edson Pinheiro de; Costa,Sergio Eduardo Gouvêa da.
ABSTRACT Companies have needs and experience new opportunities to build their structure and align organizational process to achieve energy management with higher priority facing economic and environmental issues. The objective of this paper is to present the design and modeling of the Energy Management System (EnMS) in energy-intensive industries in an enterprise engineering approach, according to the management discipline called Business Process Management (BPM). The design of the process is based on the ISO 50001 standard and in good practices cited in the scientific literature. The methodology is characterized as basic, experimental, qualitative, and oriented according to the BPM development cycle. The process design created in the “should be” format...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy management system; Energy-intensive industries; Enterprise engineering; Business process management.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200203

Thermodynamic study of asparagine and glycyl-asparagine using computational methods
Provedor de dados: 52
Autores: Kiani,Farhoush; Behzadi,Hannaneh; Koohyar,Fardad.
This work aimed to develop an ab initio procedure for accurately calculating pKa values and applied it to study the acidity of asparagine and glycyl-asparagine. DFT methods with B3LYP composed by 6-31+G(d) basis set were applied for calculating the acidic dissociation constant of asparagine and glycyl-asparagine. The formation of intermolecular hydrogen bonds between the available species and water was analyzed using Tomasi,s method. Results showed that in alkaline solutions, the cation, anion and neutral species of asparagine and glycyl-asparagine were solvated with one, two, three and four molecules of water, respectively. There was an excellent similarity between the experimentally attained pKavalues and the theoretically ones in this work.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dissociation constant; DFT; Ab initio; Atomic charge; Asparagine; Cation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300477

Biomarkers towards Ovarian Cancer Diagnostics: Present and Future Prospects
Provedor de dados: 52
Autores: Rastogi,Meenal; Gupta,Sameer; Sachan,Manisha.
ABSTRACT Ovarian carcinoma accounts for highest mortality of all gynecologic malignancies as the disease is asymptomatic until late stages. Biomarkers such as CA-125 and HE4 are being currently used for diagnosis of ovarian cancer, but they show contradicting diagnostic accuracy. Therefore other biomarkers have been investigated for early detection of this disease, but no success has been obtained and no test has yet been recommended for screening a general population. In this instance, aptamers can be effectively used to identify tumor-specific antigens for early diagnosis and targeted therapy of ovarian cancer. This article provides an overview of the biomarkers/panels being explored as well as the potential of aptamers to improve current long-term...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovarian cancer; Aptamers; DNA methylation; CA-125; HE4.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100346

Expression Analysis of Pyrenophora teres f. maculata-Responsive Loci in Hordeum vulgare
Provedor de dados: 52
Autores: Habachi-Houimli,Yosra; Cherif,Mejda; Gharsallah,Charfeddine; Sébéi,Abdennour; Makni,Mohamed; Bouktila,Dhia.
Abstract Pyrenophora teres f. maculata is the causal agent of barley spot form net blotch (SFNB), a major stubble-borne disease in many barley-growing areas worldwide. In plants, the Nucleotide-Binding Site-Leucine-Rich Repeat (NBS-LRR) gene family functions in immunity against a variety of pathogens and pests. From a pre-established set of NBS-type resistance gene candidates, we have selected three candidate genes, HvNBS10, HvNBS72 and HvNBS85, to analyze their possible involvement in P. teres f. maculata resistance. The studied genes were mapped on chromosomes 5H and 7H. Expression profiles using qRT-PCR, 48 hours after infection by P. teres. f. maculata, revealed that the transcription of all genes acted in the same direction (down-regulation) in both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spot form net blotch (SFNB); Nucleotide-binding site (NBS); Leucine-rich repeat (LRR); QRT-PCR; Hordeum vulgare.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100425

Floral biology of cacauhy (Theobroma speciosum - Malvaceae)
Provedor de dados: 52
Autores: Souza,Márcio Silva de; Venturieri,Giorgini Augusto.
In the present work, cacauhy's (Theobroma speciosum) floral biology was studied. Flower buds split their sepals at 14h reaching its maximum at 22h, but all flowers were fully opened at 6:00 h of the following morning. Stigmatic branches showed exudates, reaching maximum between 6:00 h and 10:00 h at the same day. Ligules and petal hoods were the floral parts with highest intensity of odour. Flowers were receptive along all the morning and noon of the anthesis day. Approximately 65% of the flowers were naturally pollinated, but only 0.85% of them set a fruit. Abscission occurred on its higher frequency at 6:00 h of the second day after anthesis. Controlled pollinations showed that cacauhy was self-incompatible species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Self-incompatibility; Flower behavior; Hybridization; Cocoa.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400016

Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces
Provedor de dados: 52
Autores: Zeraik,Ana Eliza; Nitschke,Marcia.
Bacterial adhesion to inert surfaces is a complex process influenced by environmental conditions. In this work, the influence of growth medium and temperature on the adhesion of Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes to polystyrene surfaces was studied. Most bacteria demonstrated the highest adhesion when cultured in TSYEA, except S. marcescens, which showed to be positively influenced by the pigment production, favored in poor nutrient media (lactose and peptone agar). P. aeruginosa adhesion to polystyrene increased at low temperatures whatever the medium used. The culture medium influenced the surface properties of the bacteria as assessed by the MATS test.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial adhesion; Biofilms; Hydrophobicity; Polystyrene.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400012

Experimental Study on the Changing Rules of Coal Fire Indictor Gases of the Whole Combustion Phases in Confined Space
Provedor de dados: 52
Autores: Lei,Baiwei; Wu,Bing; Wang,Xuyang.
ABSTRACT When the coal mine fire occurs, it is crucial to judge the fire combustion state by analyzing indicator gases concentration and changing trends of various gas ratios in order to formulate proper rescue measures. It's an effective methodology to estimate the changes of combustion state in coal mine fire accurately by the trends analysis of indicator gases and gas ratios independent of the external environment disturbs, such as air leakage and inner gases injection. However, there are few experimental researches about the changing rules of indicator gases and gas ratios at different combustion phases at present. Therefore, this paper has established a small-sized coal combustion experimental platform in confined space, on which the experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coal combustion; Indicator gases; Gas ratios; Trend analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200704

New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil
Provedor de dados: 52
Autores: Gonçalves,Dicezar; Gabriel,Jane Eire; Madeira,Humberto Maciel França; Schühli,Guilherme Schnell e; Vicente,Vânia Aparecida.
The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Molecular markers; Subclinical mastitis; Clinical mastitis; Mastitis detection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200014

Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
Provedor de dados: 52
Autores: Pujatti,Priscilla Brunelli; Simal,Carlos Jorge Rodrigues; Santos,Raquel Gouvêa dos.
Technetium-99m (99mTc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Labeling; Hemolytic activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700002

Paper-Based Microfluidics Immunoassay for Detection of Canine Distemper Virus
Provedor de dados: 52
Autores: Mazzu-Nascimento,Thiago; Donofrio,Fabiana Cristina; Bianchi,Beatriz Cutilak; Travensolo,Regiane de Fátima; Souza Junior,João Batista; Moraes,Daniel Angeli; Morbioli,Giorgio Gianini; Segato,Thiago Pinotti; Varanda,Laudemir Carlos; Carrilho,Emanuel.
ABSTRACT Paper-based devices present low-cost and are versatile, making them very attractive for clinical analysis. To manufacture those devices wax patterns are printed on paper surface and upon heating the wax permeates through the entire thickness of the paper, creating hydrophobic barriers that delimit test areas. Antibodies produced in rabbits against canine distemper virus (CDV) were physically adsorbed on the surface of gold nanoparticles (AuNPs) and incubated with CDV viral antigens, forming the immunocomplex. Anti-CDV antibodies were immobilized into the microchannels by physical adsorption, forming the test region. The test solution containing conjugated AuNPs was applied at the bottom of the microchannel and it was eluted with a phosphate buffer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paper-based devices; Wax printing; Point-of-care testing; Gold nanoparticles; Canine distemper virus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100318

Co-Production of Nattokinase and Poly (γ-Glutamic Acid) Under Solid-State Fermentation Using Soybean and Rice Husk
Provedor de dados: 52
Autores: Nie,Guangjun; Zhu,Zhubin; Liu,Fang; Nie,Zhijie; Ye,Yuchao; Yue,Wenjin.
ABSTRACTThe aim of this work was to study the co-production of nattokinase and poly (γ-glutamic acid) by Bacillus subtilis natto with soybean and rice husk under solid-state fermentation (SSF). The results showed that the size of soybean particle and rice husk significantly improved the co-production of nattokinase and poly (γ-glutamic acid), yielding 2503.4 IU/gs and 320 mg/gs, respectively in the improved culture medium composed of 16.7% soybean flour and 13.3% rice husk with 70% water content. The yields increased by approximate 7- and 2-fold factor relative to their original ones. Thus, the co-production of nattokinase and poly (γ-glutamic acid) under SSF could be considered as an efficient method to exploit agro-residues for economical production of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-production; Nattokinase; Poly (γ-glutamic acid); Rice husk; Solid-state fermentation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500718

Erratum: Apoptosis and Histopathology of the Heart after Renal Ischemia-Reperfusion in Male Rat Running title: Ischemia-Reperfusion Injury
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100900

Highly Solvent Tolerance in Serratia marcescens IBBPo15
Provedor de dados: 52
Autores: Stancu,Mihaela Marilena.
ABSTRACT The aim of this study was to investigate the solvent tolerance mechanisms in Serratia marcescens strain IBBPo15 (KT315653). Serratia marcescens IBBPo15 exhibited remarkable solvent-tolerance, being able to survive in the presence of high concentrations (above 40%) of toxic organic solvents, such as cyclohexane, n-hexane, n-decane, toluene, styrene, and ethylbenzene. S. marcescens IBBPo15 produced extracellular protease and the enzyme production decreased in cells exposed to 5% cyclohexane, n-hexane, toluene, styrene, and ethylbenzene, as compared with the control and n-decane exposed cells. S. marcescens IBBPo15 cells produced carotenoid pigments and alteration of pigments profile (i.e., phytoene, lycopene) were observed in cells exposed to 5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serratia marcescens; Solvents; Tolerance; Mechanisms.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100704

Dissolved Organic Carbon Determination Using FIA and Photo-Fenton Reaction
Provedor de dados: 52
Autores: Kondo,Márcia M.; Arcos,Maria A. S. V.; Grassi,Marco T.
The FIA-photo-Fenton system is based on the flow oxidation of the organic matter. A small amount of the sample containing H2O2 is injected into the acidic flow solution of Fe2+, which passes through a tubular PTFE reactor irradiated with UV light. The generated CO2 is quantified by a conductometric detector and is directly proportional to the dissolved organic carbon concentration in the sample. The optimization studies were performed using EDTA solutions. The average recovery of organic carbon was 83% with a relative standard deviation of 3.7% using a 1:5 molar ratio of Fe2+:H2O2, pH 2.0, 100 muL of sample injection and a liquid flow of 1 mL min-1. After optimization, the DOC concentration was quantified using 13 different organic compounds, where the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photo-Fenton reaction; Dissolved organic carbon; FIA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100013

Cysteine proteinases and cystatins
Provedor de dados: 52
Autores: Oliveira,Adeliana S.; Xavier-Filho,José; Sales,Maurício P.
This review describeds the definition, localization, functions and examples of cysteine proteinases and their protein inhibitors in vertebrate, non-vertebrate animals and plants. These inhibitors are related with defense mechanisms of plant against pests. It also describes the factors involved in the specific cysteine proteinase-cystatin interaction and high degree of affinity and large specificity in this interaction which are not only represented by the compatibility between amino acid residues of the active site involved in catalysis, but also of all amino acid residues that participante in the enzyme-inhibitor interaction.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cysteine proteinases; Cystatin; Phytocystatins.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100014

Radiopeptides from diagnosis to therapy
Provedor de dados: 52
Autores: Buscombe,John. R.
Radiopeptides are playing an increasing role in the diagnosis of disease. Initially based on the somatatostatin system, early research concentrated on identifying tumours of neuroendocrine origin. New techniques with artificial peptides are extending their role into other areas such as thrombus formation and heart disease. It has been shown that radiopeptides have excellent labelling new methods have looked at therapy starting with high activities of In-111 pentetreotide to the development of Y-90 based products and new and novel peptides and radio isotopes. Outside of PET there are the most exciting developments in nuclear medicine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopeptides; Diagnosis; Therapy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500013

Temporal genotypic diversity of Schizaphis graminum (Rondani 1852) (Hemiptera: Aphididae) in a black oats (Avena strigosa) field
Provedor de dados: 52
Autores: Lopes-da-Silva,Marcelo; Vieira,Luiz Gonzaga Esteves.
The aim of this work was to analyze the clonal diversity variation in Schizaphis graminum during a complete phenological cycle of black oats (Avena strigosa). RAPD markers were used for detection of DNA polymorphisms of each clonal lineage, derived from a single clone collected weekly during a period of four months, in a crop field of black oats, Londrina, Paraná, Brazil. The monthly genotypic diversity was estimated by Shannon Information Index (H). Only four genotypes were distinguished from 122 specimens, with one of them overly predominant in all sampling dates (&gt;60%). Another genotype, apparently a later colonizer, rapidly reached greater frequency than other genotypes on the second and third month. The results of this work suggested that temporal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; RAPD; Temporal scale.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400021

Erratum: Determination of the Effect of the Elimination Diet Applied for Overweight and Obese People with Food Intolerance on Body Composition and Biochemical Parameters
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100915

Experimental Investigation on Self-Compacting Self-Curing Concrete Incorporated with the Light Weight Aggregates
Provedor de dados: 52
Autores: G S,Rampradheep; Sivaraja,M.
ABSTRACT Self-Compacting Concrete (SCC) flows around obstructions by its self-weight to fill entirely and self-consolidate (without any need for vibration), without any part of disconnection and chunking. The eradication of the need for consolidation leads to better quality concrete and substantial improvement of running conditions. The fresh state of SCC mixes will usually have huge amount of fine fillers, including cement, and produce excessively high compressive strength concrete. In order to overcome the workability problem Super Plasticizer (SP) and Viscosity Modifying Agent (VMA) are used. Here, the workability admixtures are fixed at a constant rate of 2% based on the weight of cement. This technique examines special applications in cases of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Self-compacting concrete; Vermiculite; Diatomaceous; Internal curing; LECA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300609

The effects of intramuscular or intravenous injections of gonadotropin releasing hormone at fixed-time artificial insemination on pregnancy rates of Bos indicus beef cows
Provedor de dados: 52
Autores: Demeterco,Danilo; Henry,Darren Dwayne; Mercadante,Vitor Rodrigues Gomes; Lamb,Graham Cliff; Gaievski,Francisco Romano; Weiss,Bruno; Turbay,Gustavo Nascimento; Segui,Márcio Saporski; Weiss,Romildo Romualdo; Betiol,Melina Andreia Formighieri; Kozicki,Luiz Ernandes.
The effect of an intramuscular versus intravenous administration of gonadotropin releasing hormone (GnRH) at fixed-time AI (FTAI) on the pregnancy rates of crossbred Bos indicus beef cows was evaluated. Pluriparous nursing calv cows (n=120) were synchronized as follows: d 0 cows received a 2.0 mg injection of estradiol benzoate (EB) and insertion of a controlled intravaginal progesterone releasing device containing 0.558 g of progesterone, d 8 removal of the progesterone device , a 0.15 mg injection of prostaglandin F2α (PGF), a 1.0 mg injection of EB, and 400 IU injection of equine chorionic gonadotropin. Fifty-four hr after PGF, all cows were exposed to FTAI and a 0.084 mg injection of GnRH was administered either via Vena caudalis (n=60), or via...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonadotropin releasing hormone; Fixed-time artificial insemination; Intravenous intramuscular administration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300008

Oxidative stress parameters as biomarkers of risk factor for diabetic foot among the patients with type 2 diabetes
Provedor de dados: 52
Autores: Oliveira,Ana Carla Pozzi; Teixeira,Caio Jordão; Stefanello,Talitha Fernandes; Carrara,Marcia Aparecida; Bazotte,Roberto Barbosa; Sá-Nakanishi,Anacharis Babeto; Comar,Jurandir Fernando; Batista,Marcia Regina.
The aim of this study was to determine whether plasma levels of carbonylated proteins, total antioxidant capacity (TAC) and reduced protein thiols could be suitable biomarkers of risk factors for diabetic foot. Individuals with type 2 diabetes with normal protective sensation (normal foot group) vs. loss of protective sensation and/or signs of peripheral arterial disease and/or foot deformities and/or history of ulcers and/or neuropathic fractures and/or amputation (diabetic foot group) were compared. The diabetic foot group showed higher carbonylated protein levels (P = 0.0457) and lower levels of TAC (P = 0.0148) and reduced protein thiols (P = 0.0088), compared with the normal foot group. In general, several other parameters of risk of diabetes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic foot; Oxidative stress; Total antioxidant capacity; Reduced protein thiols; Protein carbonyls.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200009

Estimation of some heavy metals in polluted well water and mercury accumulation in broiler organs
Provedor de dados: 52
Autores: Hussein,Hussein Khamis; Abu-Zinadah,Osama Abdullah; EL-Rabey,Haddad Abdulsameih; Meerasahib,Mohammed Fareez.
The aim of this study was to investigate the relationship between the concentrations of heavy metals in well water and bioaccumulation of the most abundant metals in chicken tissues in some areas in the province of Mecca Almokaramah, Saudi Arabia. Among the heavy metals (Cd, Zn, Cr, Mn, Cu Hg, Pb and Ni) studied, mercury (Hg) revealed highest in concentration in well waters. The concentration of mercury in the ground water, beside in liver, kidney, muscle and blood samples of ten chickens from each of four poultry- production farms were estimated using atomic absorption spectrophotometer. The results showed that the kidney followed by liver had the highest bioaccumulation of mercury in all farm samples. The level of mercury in the ground water was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental toxicology; Ground water; Heavy metals; Mercury; Bioaccumulation-chicken histopathology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500007

Spatial and multi-temporal analysis of deforestation and quantification of the remnant forests on Porto Rico Island, Paraná, Brazil
Provedor de dados: 52
Autores: Campos,João Batista.
The process of land occupation by the state of Paraná practically decimated its forests. Presently, only 7% from a total area of 83.41% is covered by forests. Most of them are now concentrated in the "Serra do Mar" and in the Iguaçu National Park. With regard to forest cover the most critical region is situated in the northwestern of the state with less than 1% of its area covered by forest. This restricted area mainly consists of conservation units and the islands of the high Paraná River floodplain. The present study consists of a spatial and multi-temporal analysis of deforestation and the quantification of the remnant forests on Porto Rico island (22º45'S; 53º15'W), which have their occupation history linked to the colonization process of the region....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photogrammetry; Alluvial forest; Floodplain; Forest.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100013

Genetic diversity in passion fruit (Passiflora spp.) evaluated by RAPD markers
Provedor de dados: 52
Autores: Crochemore,Maria Lúcia; Molinari,Hugo Bruno Correa; Vieira,Luiz Gonzaga Esteves.
The objective of this study was to characterize the genetic diversity within a Passiflora collection by PCR-RAPD markers. Genetic analysis was performed in 70 accessions, representing 11 species of the genus Passiflora. The use of only five primers produced 136 reproducible polymorphic bands. The hierarchical classification showed high levels of dissimilarities between and within the species studied. A clear separation was obtained among species and accessions of P. edulis and P. edulis f. flavicarpa, which were forms of the high commercial value species. The fingerprints produced from the studied genotypes would allow the identification of improved varieties/populations in an easy, fast and inexpensive manner.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora spp.; Germoplasm; Genetic diversity; RAPD-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400005

Effect of probiotic administration on the immune response: a systematic review of experimental models in rats
Provedor de dados: 52
Autores: Silva,Viviam de Oliveira; Foureaux,Renata de Carvalho; Araujo,Thiago Santos; Peconick,Ana Paula; Zangeronimo,Marcio Gilberto; Pereira,Luciano José.
The probiotic influence on the immune system, especially under pathogenic challenge conditions, still remains controversial. To address this, a systematic review of current studies concerning the efficacy of probiotics on the immune response of rats subjected to experimental challenges was conducted. The survey was conducted using PubMed, ISI Web of Science and Scielo databases. Only studies which tested probiotics in vivo in rats were included. The experimental design, methodological quality, and results of the articles were analyzed. In total 21 articles were selected for this study. The most commonly used microorganisms in the experiments were those of the genus Lactobacillus, which was reported in 12 articles. The second most often used genus was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunity; Probiotics; Rats; Dietary supplements.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500007

Azospirillum sp . inoculation in wheat, barley and oats seeds greenhouse experiments
Provedor de dados: 52
Autores: Dalla Santa,Osmar R.; Hernández,Ramona Fernández; Alvarez,Gergina L. Michelena; Ronzelli Junior,Pedro; Soccol,Carlos Ricardo.
The aim of the present work was to evaluate the inoculation effects of Azospirillum sp. RAM-7 strain, in seeds of wheat, barley and oats cultures on yield and total nitrogen content of the grains in greenhouse experiments. Seeds inoculation with Azospirillum sp. RAM-7 strain enhanced grain yields, even so, the responses vary among the evaluated cultures. For wheat, significant increases were obtained when the inoculation was associated to 100% of the recommended nitrogen, although the treatment that received only inoculation produced 7.4% above the control, however this difference wasn't significant. For barley, the presence of the inoculant substituted 20% the recommended nitrogen fertilization. For oats the inoculation with Azospirillum sp. RAM-7 didn't...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azospirillum; Wheat; Barley; Oats; Nitrogen fixation; Plant growth promotion.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600002

Stochastic modeling for a better approach of the in vitro observed growth of colon adenocarcinoma cells
Provedor de dados: 52
Autores: Ruiz-Gómez,Miguel J.; Ruiz-Gómez,Antonio; Martínez Morillo,Manuel.
The definition of a stochastic model that reflects the cell growth and the use of computer software could be very useful in modelling the cell behaviour due to the possibility to introduce alterations in biology parameters to obtain different growth patterns without the use of laboratory material. Human colon adenocarcinoma cells were cultured and a growth curve was made by daily count of the cell number. Pielou modelling was applied for stochastic simulation of deterministic growth, making stochastic the cell division, the death rates and the transition time between division and death, by using different probabilities. A greater growth was produced when the cell division rate increased, considering the density dependence constant. In contrast, a lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon carcinoma; Cell growth; Stochastic; Deterministic; Simulation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300006

The abundance of some pathogenic bacteria in mangrove habitats of Paraiba do Norte estuary and crabmeat contamination of mangrove crab Ucides cordatus
Provedor de dados: 52
Autores: Grisi,Teresa Cristina Soares de Lima; Gorlach-Lira,Krystyna.
Study on mangrove water and sediment of Paraiba do Norte river (Northeastern Brazil) and crabmeat of Ucides cordatus was carried to investigate the relation between the abundance of bacteria in the mangrove habitat and in the crabmeat. Simultaneous collection of samples of water, sediment and crabs (U. cordatus) was done during the rainy and dry seasons at two areas of mangroves that differed in influence of tide and sewage discharge. The numbers of total heterotrophic bacteria in the mangrove water were between 0.04 and 6 log CFU 100mL-1, and in the sediment between 5 and 6 log CFU g-1. The location closer to waste discharge showed higher incidence of total heterotrophic bacteria, coliforms, Staphylococcus aureus and Salmonella spp. The counts of total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangrove ecosystem; Bacteria; Ucides cordatus; Crabmeat; Contamination.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100028

Embryo transfer in no cycling Crioula and Quarter horse breeds treated with estradiol cipionate and long-acting progesterone
Provedor de dados: 52
Autores: Kaercher,Fernanda; Siqueira,Marlon; Mercadante,Alessandro; Figueiredo,Tatiana; Weiss,Romildo Romualdo; Siqueira,Felipe; Kozicki,Luiz Ernandes.
The aim of this work was to prepare the mares for embryo transfer. In group 1 (G1,treated, n=15), recipient mares in anoestrus or in a transition period were treated with 5.0, 3.0 and 2.0 mg of estradiol cipionate at the days 0, 1 and 2 respectively, beginning at the day of ovulation (DO). From the fourth day on, the mares this group received long-acting progesterone weekly, up to the 120 day of gestation. At D8, the embryo was collected from the donor and transferred to the recipient. At D12, the ultrasonographyc diagnosis of pregnancy was carried out. The control group (G2, not treated, n=20) was formed by cycling recipient mares, displaying ovulation at each 2 to 3 days after the donors mare ovulation. The pregnancy rate was higher (p&lt;0.05) in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo transfer; Cycling and no cycling mares; Long acting progesterone; Estradiol cipionate; Mares recipient.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600009

Ultrastructural and quantitative studies of hemocytes in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Pyralidae)
Provedor de dados: 52
Autores: Falleiros,Ângela Maria Ferreira; Bombonato,Maria Terezinha Siqueira; Gregório,Elisa Aparecida.
Six circulating hemocytes cell types from Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae) larvae were identified by transmission and scanning electron microscope: prohemocytes (PR), plasmatocytes (PL) granulocytes (GR), spherulocytes (SP), oenocytoids (OE) and vermicytes (VE). The PR was the smallest cell type with a large nucleus, a cytoplasm with few organelles and a homogenous smooth surface. The PL was polymorphic and abundant, with a cytoplasm rich in organelles and a cellular surface with several cytoplasmic projections. The GR was abundant, showing two types of membrane-bounded granules (dense and structutered), glycogen, lipid droplets and a surface with philopodial projections. The SP was a large cell, with a cytoplasm full of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect; Lepidoptera; Hemocytes; Ultrastructural Morphology; Differential Hemocyte Count; Morphometry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200021

An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance
Provedor de dados: 52
Autores: Pileggi,Marcos; Pereira,Albanin Aparecida Mielniczki; Silva,Joandrei dos Santos; Pileggi,Sônia Alvim Veiga; Verma,Desh Pal S.
An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. The major modifications were concerned with turning more practical the transformation and regeneration protocols. Also we tried to improve transformation steps by increasing injured area in explants and prolonging co-cultivation with Agrobacteria (in larger concentration). A more significant selective pressure was used against non-transformed plants and bacteria. In these work we were concerned to obtain T1 and T2 seeds. The P5CS gene codes for a delta¹-pyrroline-5-carboxylate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress resistance; Cold resistance; Transgenic; Lettuce; Proline.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200013

Acid and enzymatic hydrolysis to recover reducing sugars from cassava bagasse: an economic study
Provedor de dados: 52
Autores: Woiciechowski,Adenise Lorenci; Nitsche,Saul; Pandey,Ashok; Soccol,Carlos Ricardo.
The objective of this work was to study the acid and enzymatic hydrolysis of cassava bagasse for the recovery of reducing sugars and to establish the operational costs. A statistical program "Statistica", based on the surface response was used to optimize the recovery of reducing sugars in both the processes. The process economics was determined considering the values of reducing sugars obtained at laboratory scale, and the operations costs of a cylindrical reactor of 1500 L, with flat walls at the top and bottom. The reactor was operated with 150 kg of cassava bagasse and 1350 kg of water. The yield of the acid hydrolysis was 62.4 g of reducing sugars from 100 g of cassava bagasse containing 66% starch. It represented 94.5% of reducing sugar recovery. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava bagasse; Hydrolysis; Acid; Enzyme; Reducing sugars; Economics.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300018

Using egg albumin foam to extinguish fires
Provedor de dados: 52
Autores: Alsaati,Hytham A.; Ditzler,Danielle; Burapatana,Vorakan; Tanner,Robert D.
Oil, coal and chemical fires are often difficult to put out using water. In certain hydrocarbon fires, protein foam can extinguish fires better than water by keeping air (oxygen) away from the flames and by ''blowing'' the flame away from its fuel source. Egg albumin is a relatively inexpensive protein and is representative of foaming proteins, which are candidates for use as fire suppression agents. This paper begins to deal with the effect of the foam bulk pH, foam protein concentration and generating air flow rate into the foam on the fire extinguishing time in laboratory experiments. A Bunsen burner was used to generate a small, controlled laboratory fire within a plastic container, which represented a point source in a partially open room in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein Foam; Albumin; Fire Suppression; Biodegradable Foams; Natural Gas Flame.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400029

Physicochemical and antioxidant properties of kiwifruit as a function of cultivar and fruit harvested month
Provedor de dados: 52
Autores: Pal,Ramesh Singh; Kumar,Vandana A; Arora,Sandeep; Sharma,A K; Kumar,Virendra; Agrawal,Sanjeev.
The present study was carried out to find the effect of fruit harvesting stage (October, November and December) on the physicochemical and antioxidant properties in five kiwi cultivars (Abbot, Bruno, Allison, Hayward, Monty). Results showed that soluble solid content (SSC) and pH increased while ascorbic acid (Vit C), titrated acidity (TAD) and SSC/TAD decreased in all the cultivars with delay in harvesting. Total polyphenols (TP) were decreased while total flavonoids (TF) increased in all tested cultivars with delay in harvesting. The highest concentration of TP (2.02 mg gallic acid equivalent/g fresh weight) and TF (51.12 mg catechin equivalent/100g FW) were found in cultivar 'Allison' in the month of October and December, respectively. Antioxidant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant Properties; Kiwi Fruit; Fruit Harvest Month; Physicochemical Properties.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200262

Production and properties of Xylanase from thermophilic Bacillus sp.
Provedor de dados: 52
Autores: Cordeiro,Carlos Alberto Martins; Martins,Meire Lelis Leal; Luciano,Angélica Bárbara; Silva,Roberta Freitas da.
An aerobic, thermophilic, xylanolytic bacterium was isolated from local soil. The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1. These organisms exhibited 94% levels of ribossomal DNA sequence homology. Studies on the xylanase characterisation from liquid cultures grown on beechwood xylan revealed that the enzyme retained 100% of activity for 2 hours at temperatures ranging from 30 to 50º C, while at 60, 70 and 100º C, 10%, 11% and 29% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be between 6.5 and 7.0. After incubation of crude enzyme solution for 24 hours at 25º C and at pH 5.5 to 8.0, a decrease of about 12%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylanase; Thermophilic bacterium; Bacillus sp.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600002

Protease gene shuffling and expression in Pichia pastoris
Provedor de dados: 52
Autores: Yang,Gang; Chang,Juan; Yin,Qingqiang; Wang,Erzhu; Zhu,Qun; Wang,Ping; Dang,Xiaowei; Lu,Fushan.
Four kinds of neutral and alkaline protease genes from Aspergillus oryzae and Bacillus subtilis were isolated and shuffled. The shuffled genes were selected, inserted into pGAPZαA plasmid and transformed into Escherichia coli. The gene which could express high-activity protease was selected by screening the sizes of transparent zones around the colonies on casein plates. After an ideal protease gene was selected, it was sequenced and then transformed into Pichia pastoris X33. The result showed that the base in 1022th position of shuffled protease gene was changed from thymine to cytosine, inferring that cysteine was changed to arginine in the mutant protease. After 48 h incubation for the transformed P. pastoris with the mutant or native protease genes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease gene; DNA shuffling; Pichia pastoris; Gene expression; Protease characterization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300337

Antioxidant Activity of Oral Administration of Rosmarinus Officinalis Leaves Extract on Rat's Hippocampus which Exposed to 6-Hydroxydopamine
Provedor de dados: 52
Autores: Rasoul,Arashpour; Maryam,Haji GhasemKashani; Taghi,Ghorbanian Mohammad; Taghi,Lashkarbolouki; dehghan,Rada asle.
Carnosic acid, a diterpene of Rosemarinus officinalis leaves extract (RE), has potent antioxidant activity in vitro. The dopaminergic connection of substantia nigra pars compacta to the hippocampus might be affected by oxidative stress which caused cognitive impairment observed in the early phase of Parkinson's disease (PD). Adult male Wistar rats were lesioned bilaterally by intra-nigral injection of 6-OHDA, and divided into six groups: four groups that orally given RE containing 40% of carnosic acid, at doses of 25, 50 and 100 mg/kg (treated rats) and distilled water (H2O), once daily for a period of 14 days before and after the injury. There were also two another groups as control rats which injected by normal saline and untreated lesion group. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosmarinus officinalis leaves extract; Antioxidant enzymes; Hippocampus; 6-Hydroxydopamine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100312

Mimosine and cyclophosphamide: a potential new combination therapy used to prevent tumor development
Provedor de dados: 52
Autores: Dipe,Vânius Vinícius; Gotardo,André Tadeu; Haraguchi,Mitsue; Górniak,Silvana Lima.
The effects of mimosine (MI), which is an amino acid that is derived from Leucaena leucocephala, were evaluated on the growth of ascitic Ehrlich tumors, and the effects of the combination treatment of MI and cyclophosphamide (CY) on tumor growth were also assessed. Mice were divided into groups that received the following treatments over the course of 20 days: phosphate buffer solution (CO), MI, Ehrlich cells (E), E plus CY (EC), E plus MI (EM) and E plus MI and CY (EMC). No signs of toxicity were detected in the mice from the MI group. The mice from the EMC group showed reductions in body weights when compared with those from the E group. The animals from the EC, EM and EMC groups showed reductions in ascitic volume compared with those from the E group....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucaena leucocephala; Mimosine; Ehrlich tumor; Cyclophosphamide; Anticancer activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600010

The occurrence of Aeromonas spp. in the bottled mineral water, well water and tap water from the municipal supplies
Provedor de dados: 52
Autores: Scoaris,Denise de Oliveira; Bizerra,Fernando Cezar; Yamada-Ogatta,Sueli Fumie; Abreu Filho,Benício Alves de; Ueda-Nakamura,Tânia; Nakamura,Celso Vataru; Dias Filho,Benedito Prado.
The aim of this work was to study the occurrence of Aeromonas sp in the bottled mineral water, well water and tap water from the municipal supplies. Positive samples were found for Aeromonas spp. 12.7% from the mineral water, 8.3% from the artesian water and 6.5% from the tap water. The recovery of Aeromonas spp. was significantly higher in the bottled mineral and artesian water than in the tap water from municipal supplies. The occurrence of the Aeromonas spp. did not correlate significantly with the contamination indicator bacteria (i.e. total coliforms) in the artesian water samples. However, a significant correlation was found between Aeromonas spp. and total coliforms in the both mineral water and tap water samples. The presence or absence of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas; Drinking water; Indicator bacteria; Coliforms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500022

Changes in electrophoretic profiles of Ipomoea batatas (sweet potato) induced by gamma radiation
Provedor de dados: 52
Autores: Lage,Celso Luiz Salgueiro; Vasconcellos,Alexandre Guimarães; Silva,Nina Claudia Barboza da; Esquibel,Maria Apparecida.
The ability of nodal segments of Ipomoea batatas to differentiate shoots and roots was evaluated after gamma irradiation. Shoot differentiation was less sensitive to irradiation than roots. However, at 90 Gy, no shoot was able to regenerate a new plant; in contrast 76 % of the roots from irradiated nodal segments continued to grow. The gamma radiation also induced changes in electrophoretic profiles of peroxidases of storage roots. Plants originated from irradiated storage roots presented changes in leaf peroxidase profiles very similar to those produced by leaves directly irradiated. The peroxidase profile of absorbent roots from irradiated storage roots was different from that obtained from directly irradiated absorbent roots.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxidases; Gamma radiation; Sweet potato; Ipomoea batatas; Convovulaceae.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200008

High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells
Provedor de dados: 52
Autores: Ohtsuki,Takashi; Himeji,Mika; Fukazawa,Harumi; Tanaka,Miho; Yamamoto,Hisako; Mimura,Akio.
Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 mg/L of wogonin at two week. In addition, the production of baicalin was drastically increased to 1000 mg/L level at 3-week culture, and the extremely high production rate (339 mg/L•week) was obtained. In the comparison of total antioxidative activities among baicalein, baicalin and wogonin, evaluated by thiocyanate method, it was suggested that the location of hydroxyl groups both at 5- and 6-position contributed to enhancement of radical scavenging activity, and/or methoxylation at 8-position diminished...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Flavonoids; Liquid culture; High-yield production; Scutellaria baicalensis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200005

Influence of chromium salts on increased lipid peroxidation and differential pattern in antioxidant metabolism in Pistia stratiotes L
Provedor de dados: 52
Autores: Upadhyay,RishiKesh; Panda,Sanjib Kumar.
In this work, the changing effect of different concentrations (0, 0.01, 0.1, 1, 10mM) of hexavalent and trivalent chromium on different biochemical parameters along with antioxidant enzymes was investigated on water lettuce (Pistia stratiotes L.) in order to know the possible involvement of this metal in oxidative injury, besides the activities of antioxidant enzymes leading to biochemical and oxidative aberration induced by elevated concentrations. Both in roots and shoots, Cr produced a significant increase in enzymic and non-enzymic antioxidants, except in catalase (CAT) activity where a strong accumulation of hydrogen peroxide was indicated, suggesting an imposition of oxidative stress. The observation showed an uptake of chromium by P. stratiotes L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biochemical alterations; Chromium stress; Lipid peroxidation; Oxidative stress; Pistia stratiotes L; Reactive oxygen species.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500018

Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG
Provedor de dados: 52
Autores: Sado,Ricardo Yuji; Matushima,Eliana Reiko.
The aim of this study was to evaluate the inflammatory response kinetics after experimental inoculation with BCG in the primitive Arius sp. fish. The BCG was applied through the intramuscular injection in the caudal peduncular region, and the samples were collected for the analyses at days 1, 3, 7, 14, 21, and 33 post-injection. Acute phase inflammatory infiltrate was characterized by the predominant mononuclear cells, intersticial edema, and muscular tissue necrosis. As the inflammatory response evolved, a large number of multinuclear giant cells were formed containing the BCG. These giant cells were positive for the S100 protein at the histochemical analysis, which demonstrate the macrofage activity, confirmed by the ultra-structural analysis showing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Arius; Phylogeny; BCG; Inflammation; Giant cells.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500009

Effect of female weight on reproductive potential of the predator Brontocoris tabidus (Signoret, 1852) (Heteroptera: Pentatomidae)
Provedor de dados: 52
Autores: Oliveira,Isaias; Zanuncio,José Cola; Serrão,José Eduardo; Zanuncio,Teresinha Vinha; Pinon,Tobias Baruc Moreira; Fialho,Maria do Carmo Queiroz.
The objective of this work was to determine the fecundity of the predator Brontocoris tabidus (Signoret) (Heteroptera: Pentatomidae) females of two weight classes aiming to define, which one presented higher productivity in the laboratory. Males and females of B. tabidus were reared from nymphs fed with Tenebrio molitor L. (Coleoptera: Tenebrionidae) pupae in laboratory. Females of B. tabidus weighting 95 to 150 mg and those with 160 to 220 mg constituted two treatments. Oviposition period and numbers of egg masses, eggs and nymphs per female of B. tabidus were higher in the treatment with heavier females, while the periods of preoviposition, between egg mass laying, egg incubation and number of eggs per egg mass, besides the percentage of nymphs hatched...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asopinae; Predatory bugs; Mass rearing; Biological control; Reproductive potential.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200017

Isolation and characterization of a newly isolated Pseudomonas mutant for protease production
Provedor de dados: 52
Autores: Dutta,Jayati Ray; Banerjee,Rintu.
A potent bacterium for extracellular protease production was isolated from local soil and identified as Pseudomonas sp. RAJR 044. A mutant of this strain JNGR 242 with protease productivity 2.5 fold higher was obtained by ultraviolet irradiation under experimentally optimized conditions of pH 7.0, temperature of 34ºC, inoculum volume of 1.0 mL and incubation time of 24 hours. Comparative analysis of the chemical characteristics i.e. assimilation of carbon and nitrogen sources were also carried out. Maximum growth of the mutant strain in 2% gelatin agar plate was obtained in presence of dextrose (2%), maltose (2%), ammonium sulfate (2%) and potassium nitrate (2%) whereas, that of the parent strain was found in sucrose (2%) and ammonium nitrate (2%). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular protease; Purification; Kinetic characterization; Ultraviolet mutagenesis; Pseudomonas sp.; Physicochemical.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100005

Temporal and spatial variation on heavy metal concentrations in the bivalve Perna perna (LINNAEUS, 1758) on the northern coast of Rio de Janeiro State, Brazil
Provedor de dados: 52
Autores: Ferreira,Alexandre Gomes; Machado,André Luís dos Santos; Zalmon,Ilana Rosental.
Heavy metal (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) concentrations were studied in Perna perna from three beaches (Barra do Furado, Buena and Ponta do Retiro) on the northern coast of Rio de Janeiro State by ICP-AES. The average concentration was 0.3 ± 0.04 (Cd); 1.9 ± 0.6 (Cr); 6.1 ± 0.7 (Cu); 1,130 ± 113 (Fe); 22 ± 3.2 (Mn); 9.3 ± 4.6 (Ni); 0.4 ± 0.2 (Pb); 44 ± 5.8 (Zn) µg.g-1 dry weight. There were no significant difference for almost all the studied metals in relation to sex. Although, significant spatial variations (p &lt; 0.05) occurred for Cr, Pb and Ni with higher values for Barra do Furado. Temporal variations were significant (p &lt; 0.05) at the three beaches, however, no trends of accumulation were observed. The metal concentrations were similar to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metals; Mollusk; Spatial variation; Temporal variation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200020

Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes
Provedor de dados: 52
Autores: Duarte,Ana Rodrigues; Duarte,Débora Maria Rodrigues; Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Lima-Filho,José Luiz de; Porto,Ana Lúcia Figueiredo.
The partial characterization and purification of milk clotting enzyme obtained from the (root latex) of Jacaratia corumbensis O. kuntze was studied, by fractional precipitation with ammonium sulphate and ion exchange chromatography. The ammonium sulphate precipitate showed five fractions (AS1- 0-20%; AS2 - 20-40%; AS3 - 40-60%; AS4 - 60-80%; AS5 - 80-100%) and among the fractions obtained, the 40-60% fraction (AS3) showed the highest milk clotting activity with a purification factor of 1.2 fold in relation to the crude extract. This fraction when applied on Mono Q column yielded two protein peaks (p1 and p2), but p1 pool showed the best milk-clotting activity. The optimal pH for the crude and partially purified extract was 6.5 and 7.0, respectively. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jacaratia corumbensis O. kuntze; Milk clotting enzyme; Characterization; Purification; Vegetable enzyme.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100001

Mycorrhizal efficiency in acerola seedlings with different levels of phosphorus
Provedor de dados: 52
Autores: Balota,Elcio Liborio; Machineski,Oswaldo; Stenzel,Neusa Maria Colauto.
The objective of this work was to evaluate the response of acerola seedlings to arbuscular mycorrhizal fungi (AMF) with different levels of phosphorus (P) additions. The experiment was carried out in greenhouse conditions, in a randomized factorial design with four treatments of AMF (control without AMF inoculation; Gigaspora margarita; Glomus manihotis; and Glomus clarum) and four phosphorus treatments (0, 30, 90, 270 mg kg-1 soil) in four replicates. The acerola plants were harvested after 120 days of the experiment. There were significant effects of arbuscular mycorrhizal fungi inoculation on the plant growth and nutrient contents in the plant shoots, mainly under lower P soil levels. Mycorrhizal plants presented a decrease of 55% of manganese content...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malpighia emarginata D.C.; Mycorrhizal inoculation; Plant growth; Mn toxicity alleviation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300005

Research on Value Assessment and Compensation for Health Hazards of Urban Air Pollution-A Case Study of Urumqi
Provedor de dados: 52
Autores: Yu,Chen; Hui,Sun.
ABSTRACT With the acceleration of urbanization and industrialization, urban air pollution has become a serious threat to the health of urban residents. In this study, to investigate health hazards caused by air pollution for urban residents, concentrations of main air pollutants and annual coal consumption amounts during the period from 2000 to 2013 were analyzed. Our results showed that economic losses of Urumqi caused by air pollution amounted to 63.155 million yuan in 2013, accounting for 0.2 ‰ of its GDP and 5.7% of public utility expenditures for that year. The compensation mechanism analysis suggested that it is necessary to further improve the health care system and increase corporate environmental taxes. More environmental health protection taxes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollution; Health hazard; Compensation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200700

Effects of exposure to Diazinon on the lung and small intestine of Guinea pig, histological and some histochemical changes
Provedor de dados: 52
Autores: Rady,Mohamed Ibrahim.
The present study aimed to evaluate the histological changes and adverse effects on the total protein and general carbohydrates in the lung and small intestine of guinea pig "Cavia porcellus" induced by the exposure to sub lethal doses of the organophosphate insecticide diazinon. Primarily, diazinon insecticide oral LD50 was investigated after administrating orally different doses in guinea pig and was determined as 213 mg/Kg bw., the animals exposed to sub lethal doses of this insecticide revealed histological changes in both lung and intestine in a dose dependent manner. Also, significant decrease in the total protein and general carbohydrates in the lung and intestine were mostly observed, especially with the higher doses of the insecticide in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diazinon; Guinea pig; Lung; Intestine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200008

Scintigraphic imaging of focal hypoxic tissue: development and clinical applications of 123I-IAZA
Provedor de dados: 52
Autores: Wiebe,Leonard I.; McEwan,Alexander J. B.
Affected tissues in a number of diseases, including cancer, stroke, cardiac infarction and diabetes, develop focal tissue hypoxia during their progression. The presence of hypoxic tissue may make the disease refractory to therapy, as in the case of solid tumor therapy using low LET ionizing radiation. In other pathologies, the detection of viable but hypoxic tissues may serve as a prodromal indicator of developing disease (e.g. diabetes),or as a prognostic indicator for management of the disease (e.g. stroke). Over the past two decades, a number of hypoxia radioimaging agents have been developed and tested clinically. Of these, 18F-Fmiso and 123I-IAZA are the most widely used radiotracers for PET and SPECT/planar imaging, respectively. IAZA and Fmiso are a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scintigraphic Imaging; Hypoxic Tissue; Clinical Applications; 123I-IAZA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500010

Technetium-99m ceftizoxime kit preparation
Provedor de dados: 52
Autores: Diniz,Simone Odília Fernandes; Siqueira,Cristiano Ferrari; Nelson,David Lee; Martin-Comin,Josep; Cardoso,Valbert Nascimento.
The aim of this work was to prepare a kit of 99mTc-ceftizoxime (99mTc-CFT), with stability and biological activity preserved, able to identify a septic focus (E. coli) in the experimental infection model in rats. The preparation of the CFT kit involved the use of lyophilized solutions containing the antibiotic ceftizoxime and the sodium dithionite reducing agent (6.0 mg/mL). After lyophilization, the kit was reconstituted with 1.0 mL of sodium 99mTc-pertechnetate solution (Na99mTcO4-) with an activity of 370 MBq. The solution was boiled for 10 min and filtered through a cellulose ester filter. The labeling efficiency was on the order of 92%, remaining stable for six hours and the kit remained stable for two months. The biological activity of the 99mTc-CFT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tc-99m Ceftizoxime; Infection; Gamma scintigraphy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700014

Feeding ecology of juvenile dog snapper Lutjanus jocu (Bloch and Shneider, 1801) (Lutjanidae) in intertidal mangrove creeks in Curuçá estuary (Northern Brazil)
Provedor de dados: 52
Autores: Monteiro,Dijane Pantoja; Giarrizzo,Tommaso; Isaac,Victoria.
The diet and feeding ecology of juvenile dog snapper (Lutjanus jocu) were investigated in 92 specimens collected in four intertidal mangrove creeks of Curuçá estuary, Northern Brazil, between September 2003 and July 2004. No significant differences in total length were found between the sampling months. Feeding intensity was high as indicated by high stomach fullness index and low vacuity index. The most important prey was Penaeidae, followed by Grapsidae and Porcellanidae. The diet of juvenile dog snapper showed clear seasonal differences. Specimens from dry season (September and November) and dry / wet season transition (January), were specialists feeding exclusively on Penaeidae. However, the specimens from wet season (March and May) and wet /dry season...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estuary; Feeding habits; Feeding strategy; Juvenile fish; Lutjanus jocu; Intertidal mangrove creek.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600014

Physical and biological dosimetry for risk perception in radioprotection
Provedor de dados: 52
Autores: Amaral,Ademir.
The increase in ionizing radiation (IR) applications, especially nuclear, has been followed by the growth of public concern for the potential associated risks. The public’s perception of such risks is often based on the philosophy that IR is harmful at any exposure level. On the other hand, although radiation workers have knowledge about the nature of IR and its potential health effects, the relationship between absorbed dose and risk is not well understood, principally for low doses. This report presents an overview of physical and biological dosimetry as complementary methodologies, as well as their possible contribution for improving risk perception in radioprotection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodosimetry; Physical Dosimetry; Radioprotection; Public perception.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700034

The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli
Provedor de dados: 52
Autores: Agostinho,Raquel Terra; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Missailidis,Sotiris; Bernardo-Filho,Mario.
This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood constituents; Escherichia coli; Ganoderma lucidum; Stannous chloride; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700025

Life Cycle Observations on Amphilocus neapolitanus (Della Valle, 1853) (Crustacea, Amphipoda) Associated with Sargassum cymosum C. Agardh, 1820 in Ubatuba, (SP), Brazil
Provedor de dados: 52
Autores: Leite,Fosca Pedini Pereira.
Day and night densities, reproductive biology and life cycle of Amphilocus neapolitanus (Della Valle, 1853), associated with Sargassum cymosum C. Agardh, 1820, were studied during 13 months. The density of juveniles, males and females was higher in night samples than in day samples. Sex ratio was strongly favorable toward the females. Ovigerous females were present in similar densities in both the periods while the non-ovigerous females were predominant at night. The egg number was not correlated with the size of the females. Breeding occurred throughout the year.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population structure; Reproductive biology; Life cycle; Amphipoda; Sargassum.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300014

Feeding of Eigenmannia trilineata (Pisces, Sternopygidae) (Lopez &amp;amp; Castello, 1966), in the upper Paraná River floodplain, Brazil
Provedor de dados: 52
Autores: Peretti,Danielle; Andrian,Izabel de Fátima.
Studies were performed on various aspects related to the feeding of Eigenmannia trilineata in the upper Paraná River floodplain, from June 1993 to July 1994, at four places with different types of bottom and waters speed. A number of 279 fish had its stomachal contents analyzed by occurence and point methods in which it was applied on its frequencies the feeding index. Two hundred an sixty two specimens were utilized to the analysis of the feeding activity that it was based on frequency and middle degree of stomach repletion. For the morfological analyse utilized 12 individuals. It was verified fourteen items on species diet which it was found chiefly Chironomidae (Diptera) and detritus. Seasonal, area variations were verified in feeding activity and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding; Eigenmannia trilineata; Pisces; Paraná River.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100011

The effects of low level laser therapy on injured skeletal muscle
Provedor de dados: 52
Autores: Renno,Ana Claudia Muniz; Assis,Lívia; Peres,Beatriz; Rodrigues,Natalia Camargo; Brunelli,Roberta de Matos; Toma,Renata Luri; Medalha,Carla Christina; Ribeiro,Daniel Araki.
The main purpose of the present study was to investigate the effects of low-level laser therapy (LLLT) used in two different fluencies on injured skeletal muscle after cryolesion by means of histopathological analysis and immunohistochemistry for COX-2. A total of sixty male Wistar rats were randomly distributed into three groups: injured animals without any treatment; 808 nm laser treated group, at 10 J/cm² and 808 nm laser treated group, at 50 J/cm². Each group was divided into two different subgroups (n=10) on days 6 and 13 post-injury. The results showed that the animals irradiated with laser at 10 J/cm² or 50 J/cm² presented the areas with cell infiltrate and pointed out to minor and mild areas with destroyed zones compared with the control group....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low level laser therapy; Rat; Muscle repair; COX-2.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100008

Erratum: Hepatoprotective Role of α-Lipoic acid and Thymoquinone in Acetaminophen- Induced Liver Injury: Down-Regulation of COX-2 and flt-1 Expression
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100911

Helminths of the marine tucuxi, Sotalia fluviatilis (Gervais, 1853) (Cetacea: Delphinidae), in northern Rio de Janeiro State, Brazil
Provedor de dados: 52
Autores: Melo,Osana Prado; Ramos,Renata Maria Arruda; Di Beneditto,Ana Paula Madeira.
The objectives of this work were to reporte the helminths of the marine tucuxi, Sotalia fluviatilis (Cetacea: Delphinidae) in northern Rio de Janeiro State; and to check if the infestation rate varied with the host characteristics (sex and maturity) and year seasons. From 1989 to 2002, 80 marine tucuxis that were by-caught in fisheries were examined and four parasites were recorded: Braunina cordiformis, Anisakis typica, Halocercus brasiliensis and Nasitrema sp. The parasitism in the marine tucuxi corroborated its feeding habits and there were no infestation rate differences between males and females, youngs and adults, as well as between year seasons. This work reports for the first time the occurrence of the genus Nasitrema in the marine tucuxi.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sotalia fluviatilis; Marine tucuxi; Helminths; Rio de Janeiro State; Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100017

Embryo development alteration in rats treated with lapachol
Provedor de dados: 52
Autores: Maganha,Juliana; Rocha,Evelise de Souza; Brandão,Marcos Antônio Fernandes; Peters,Vera Maria; Guerra,Martha de Oliveira.
Lapachol, a naphthoquinone extracted from plants of the genus Tabebuia (family Bignoneaceae), showed multiple therapeutic activities. Pregnant Wistar rats were treated with Lapachol from the 1st to the 4th (pre-implantation period) and from 5th to 7th (implantation period) post insemination day (PID). Mothers were sacrificed on the 5th or on the15th PID. Number of corpora lutea, preimplantation embryo, blastocysts, live and dead fetuses and resorptions were counted. There were no signs of maternal toxicity. The number and the morphology of embryos, during oviduct development (pre-implantation period), did not seem to be affected by this drug, but during the implantation period, lapachol was toxic causing the death of embryos and intrauterine growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lapachol; Embryo development; Rat; Reproductive toxicity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700010

Brain Region Specificity of Mitochondrial Biogenesis and Bioenergetics Response to Nrf2 Knockdown: A Comparison Among Hippocampus, Prefrontal Cortex and Amygdala of Male Rat Brain
Provedor de dados: 52
Autores: Khalifeh,Solmaz; Khodagholi,Fariba; Shaerzadeh,Fatemeh; Ghazvini,Hamed; Zarrindast,Mohammad-Reza; Azizi,Vahid.
ABSTRACT Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) has been identified as the well-known coordinator of intracellular antioxidant defense system. Herein, we aimed to evaluate the effects of Nrf2 silencing on mitochondrial biogenesis markers peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), nuclear respiratory factor-1(NRF-1), mitochondrial transcription factor A (TFAM) and cytochrome c as well activities of two enzymes citrate synthase (CS) and malate dehydrogenase (MDH) in three brain regions hippocampus, amygdala, and prefrontal cortex of male Wistar rats. Small interfering RNA (siRNA) targeting Nrf2 was injected in dorsal third ventricle. Next, western blot analysis and biochemical assays were used to evaluation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nrf2; Antioxidant defense system; Citrate synthase; Malate dehydrogenase; PGC1-α; TFAM.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100428

Shrimp farming as an alternative to artisanal fishermen communities: the Case of Patos Lagoon, Brazil
Provedor de dados: 52
Autores: Cavalli,Ronaldo Olivera; Wasielesky Jr,Wilson; Peixoto,Silvio; Poersch,Luís Henrique da Silva; Santos,Marcos Henrique Silva; Soares,Roberta Borda.
The shrimp Farfantepenaeus paulensis is considered a suitable species for culture in the Patos Lagoon estuary, southern Brazil, where its catch has been decreasing in the last few years. Considering the potential of estuarine areas for shrimp farming, the application of low-cost structures to culture shrimp, such as the pen enclosures, is being proposed as an alternative source of income for artisanal fishermen and farmers living near Patos Lagoon. From an economical standpoint, these alternative systems present several advantages over the traditional pond-based culture systems since land, water pumping and artificial aerations are not required, high natural productivity is available and inexpensive materials are employed in the construction of grow-out...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shrimp farming; Alternative culture; Pen enclosure; Farfantepenaeus paulensis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500016

The investigation of cytogenetic and oxidative effects of diffractaic acid on human lymphocyte cultures
Provedor de dados: 52
Autores: Demir,Leyla; Toğar,Başak; Türkez,Hasan; Sozio,Piera; Aslan,Ali; Stefano,Antonio Di.
Diffractaic acid (DA) is a naturally occurring depside derivative found in several lichen species. It has a wide range of important biological effects such as analgesic and antiviral properties, although its cytotoxic, cytogenetic and oxidative effects have not been investigated in human blood tissue yet. Therefore, increasing concentrations (1, 5, 10, 25, 50, 100 and 200 mgL-1) of DA was added into human whole blood cultures. 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay was used to assess the cell viability and/or cytotoxicity and genotoxic damage potential of DA using chromosome aberration (CA) and micronucleus (MN) tests were performed. In addition, oxidative alterations were determined by the total antioxidant capacity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diffractaic acid; Cytotoxicity; Human blood; Genotoxicity; Oxidative status.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100075

The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil
Provedor de dados: 52
Autores: Mariano,Adriano Pinto; Crivelaro,Sérgio Henrique Rezende; Angelis,Dejanira de Franceschi de; Bonotto,Daniel Marcos.
This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioremediation; Soil; Groundwater; Diesel oil; Biostimulation; Vinasse.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400030

Spatial partition of artificial structures by fish at the surroundings of the conservation unit - Parque Estadual da Ilha Anchieta, SP, Brazil
Provedor de dados: 52
Autores: Cunningham,Patricia Teresa Monteiro; Saul,Andreia de Carvalho.
The aim of this work was to study the spatial partition dynamics of fish at artificial structures. Holed structured concrete blocks were used to construct eight identical artificial structures and disposed between 3m-6m depths. Installation was made in two steps during 1996 (May/June and November/December) and daily observations were carried out during 30 consecutive days SCUBA diving. The artificial reef areas were used in discriminated ways by the fish community and was most probably influenced by several factor, mainly biotic. The results of the Krustal-Wallis test led to the refutation of the hypothesis that the artificial structure spaces were shared and randomly used by fish.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish community; Artificial reefs; Space partition; Coastal area; Brazil; Conservation unit.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100015

Biodegradation of diesel oil by yeasts isolated from the vicinity of suape port in the state of Pernambuco - Brazil
Provedor de dados: 52
Autores: Miranda,Rita de Cássia; Souza,Cynthia Silva de; Gomes,Edelvio de Barros; Lovaglio,Roberta Barros; Lopes,Carlos Edison; Sousa,Maria de Fátima Vieira de Queiroz.
The aim of this work was to investigate the potential of the diesel oil degrading yeasts to use them in bioremediation of areas contaminated by this pollutant. The cultures, identified as Rhodotorula aurantiaca UFPEDA 845 and Candida ernobii UFPEDA 862, were selected at the initial stage. In the course of the biodegradation assays, C. ernobii degraded tetradecane, 5 methyl-octane and octadecane completely and decane (60.8%) and nonane (21.4%) partially whilst R. aurantiaca presented degradation percentages of 93% for decane, 38.4% for nonane and 22.9% for dodecane.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Yeasts; Diesel oil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100018

Effect of soil contaminated by diesel oil on the germination of seeds and the growth of Schinus terebinthifolius Raddi (Anacardiaceae) Seedlings
Provedor de dados: 52
Autores: Bona,Cleusa; Rezende,Igor Mendonça de; Santos,Gedir de Oliveira; Souza,Luiz Antônio de.
The effect of soil polluted by diesel oil on the germination of seeds and the growth of Schinus terebinthifolius Raddi seedlings was analyzed at different times after contamination of the soil. The experiments were conducted under greenhouse conditions, with four treatments and five repetitions. The four treatments included: soil contaminated 30 (T30), 90 (T90) or 180 (T180) days before planting as well as a non-polluted soil (T0) (control). Soil saturated to 50% of its maximum retention capacity (MRC) was contaminated with diesel oil at a rate of 92.4 mL per kg. The germination rate and germination speed index (GSI) were significantly affected only in T30. The development of the plants was affected significantly in all the treatments, with reductions of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aroeira; Biomass production; Eophyll; Residual contamination.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600025

Crude Fermented Extract Containing Gibberellic Acid Produced by Fusarium moniliforme is an Alternative to Cost Reduction in Biofactories
Provedor de dados: 52
Autores: Costa,Jefferson da Luz; Silva,André Luís Lopes da; Gollo,André Luiz; Brondani,Gilvano Ebling; Santos,Leandro Freire dos; Rodrigues,Cristine; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
ABSTRACT Nidularium procerum and Nidularium innocentii (Bromeliaceae) were cultivated in vitro on media supplemented with different sources and levels of GA3 (gibberellic acid). These sources were the commercial powder (analytical degree) and fermented extract obtained by Fusarium moniliforme via solid state fermentation. The in vitro elongation and rooting of these plants were evaluated after 50 days of cultivation. The GA3 present in the fermented extract possess the same effect of purified GA3 (analytical degree) for the increase of the height of aerial part of shoots of N. innocentii, but not for the N. procerum being the GA3 fermented extract in a lesser degree. The GA3 fermented extract influences negatively the rooting in N. innocentii, while GA3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shelf life; Bromeliad; Gibberellin; Solid state fermentation; Micropropagation; Biofactory.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100427

Establishment of an Embryonic Tip Regeneration System of Soybean
Provedor de dados: 52
Autores: Si-yan,LIU; Guang-long,LI; Jing,QU; Shu-yan,GUAN; Dan,YAO; Pi-wu,WANG; Si-chen,LIU; Yang,JIA; Peng-fei,GUAN; Long-qing,WANG.
ABSTRACT In order to establish an optimal soybean embryonic tip regeneration system that can serve as soybean genetic transformation receptor, and be used for the study of genetic function verification, the influences of single factor on the adventitious bud of embryonic tip induction, elongation and rooting stage, are researched and compared.The single factors includes seeds soaking time, different kinds of hormones, different concentration of hormone and different concentration of sucrose. By one-way ANOVA and LSD ad hoc test , the results show that, for the embryonic tip adventitious bud induction stage, 12h is the optimal seeds soaking time, 2.0mg·L-1 is the optimal concentration of 6-Benzyl Aminopurine(6-BA), for the embryonic tip adventitious bud...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Induction; Elongation; Rooting; Single factor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200204

Synergic interaction between ascorbic acid and antibiotics against Pseudomonas aeruginosa
Provedor de dados: 52
Autores: Cursino,Luciana; Chartone-Souza,Edmar; Nascimento,Andréa Maria Amaral.
Studies were carried out on in vitro combination of ascorbic acid (AA) with six antibiotics against 12 multi-resistant Pseudomonas aeruginosa isolates. Synergic activity was detected with AA chloramphenicol, kanamycin, streptomycin and tetracycline. Indifference was observed to any antibiotics and antagonism only for chloramphenicol. Results indicated that multiresistant P. aeruginosa was affected by combination of AA and antibiotics. Future research on ascorbic acid-antimicrobial interactions may find new methods to control strains of multiresistant P. aeruginosa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbic acid; Antibiotic; Synergy; Pseudomonas aeruginosa.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300007

Population structure and reproduction of Deuterodon langei travassos, 1957 (Teleostei, Characidae) in a neotropical stream basin from the Atlantic Forest, Southern Brazil
Provedor de dados: 52
Autores: Vitule,Jean Ricardo Simões; Braga,Marcelo Rennó; Aranha,José Marcelo Rocha.
Aspects of the biology of D. langei were studied at different sites along a longitudinal gradient formed by the Ribeirão stream basin, a Neotropical stream of the Atlantic Forest, southern Brazil. Differences were observed in population structure and reproduction along the longitudinal gradient and during the study period. Juvenile fishes occurred in high abundance, mainly in the downstream site after the rainy months. Adults occurred mainly in the intermediate and upstream sites. During their life cycle, adults optimise their reproductive strategy by concentrating the reproductive period with total spawn in a short time interval before summer rains dragged the juvenile, larval forms and/or eggs downstream. The downstream site was characterized by a wide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characins; Tropical fish; Longitudinal segregation; Distribution; Reproductive period; Serra do Mar.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600014

Changes of the Total Lipid and Omega-3 Fatty Acid Contents in two Microalgae Dunaliella Salina and Chlorella Vulgaris Under Salt Stress
Provedor de dados: 52
Autores: Rismani,Sharare; Shariati,Mansour.
ABSTRACT Effect of salt stress on biomass, cell number, contents of total lipid, omega-3 fatty acids, including ALA (Alpha Linolenic Acid), EPA (Eicosapentaenoic Acid) and DHA (Docosahexaenoic Acid) and their biosynthetic pathway intermediates (palmitic acid, stearic acid, oleic acid and linoleic acid) of two microalgae Dunaliella salina and Chlorella vulgaris were investigated. Dilution stress from 1.5 to 0.5 M NaCl and salt stress from 1.5 to 3 M NaCl were incorporated into the D. salina medium. Salt stress of 200 mM NaCl was also applied to C. vulgaris culture. Results indicated that increasing salt concentration resulted in the reduced growth rate of C. vulgaris and substantial increase of the total lipid content in both species. Proper growth rate of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dunaliella; Chlorella; Salt stress; Lipid; Omega-3.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100432

Occurrence of Larvae and Juveniles of Eucinostomus argenteus, Eucinostomus gula, Menticirrhus americanus, Menticirrhus littoralis, Umbrina coroides and Micropogonias furnieri at Pontal do Sul beach, Paraná
Provedor de dados: 52
Autores: Godefroid,Rodrigo Santiago; Santos,César; Hofstaetter,Marion; Spach,Henry Louis.
Despite its importance, most ichthyoplankton studies in the South-Southeastern coast of Brazil, have been accomplished on the continental shelf. Regarding the beach environment, recognized as a nursery, little is known about the ichthyoplankton. We analyzed 288 samples collected with a 18 by 2 m seine net, 1 mm mesh and a conical plankton net, with a mesh of 300 mum and a 60 cm mouth. Eucinostomus argenteus comprised 78 % of the larvae and juveniles caught in the samples. The occurrence of gerreids was strongly concentrated in the summer, while the Menticirrhus littoralis, Menticirrhus americanus and Umbrina coroides were present during all seasons. M.littoralis was most abundant during spring, while M. americanus and U. coroides dominated during winter....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish larvae; Surf Zone; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400012

Total Phenolic Contents and Antioxidant Activity in Oxidized Leaves of Mate (Ilex paraguariensis St. Hil)
Provedor de dados: 52
Autores: Molin,Rochele Fernanda; Dartora,Nessana; Borges,Ana Cláudia Piovezan; Gonçalves,Itamar Luís; Di Luccio,Marco; Valduga,Alice Teresa.
Ilex paraguariensis is a native species to South America. Its economic importance is in the use of its leaves and twigs in the processing of a product popularly known as "chimarrão". The aim of this study was to evaluate the correlations between some physico-chemical process parameters in the oxidation of I. paraguariensis leaves oxidation. The leaves of Maté with age 1.0, 6.5 and 12.0 months were subjected to an oxidation process for 3 h under different relative humidity conditions (80, 90 and 99%) at 26°C. The extracts were evaluated using the experimental design techniques to maximize the oxidation conditions for the total polyphenols, antioxidant capacity, water activity and color. The condition of 90% humidity and leaf age 6.5 months resulted in lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba-mate; Oxidative process; Physicochemical parameters; Antioxidant activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600997

Compression induced intercellular shaping for some geometric cellular lattices
Provedor de dados: 52
Autores: Calbo,Adonai Gimenez; Nery,Amauri Alves.
The wall perimeter fraction, which contact neighboring cells, was named compression ratio (alpha). A zero compression ratio indicates maximum intercellular (air) volume (vG, v/v) and neglectable contact among cells, while alpha=1 indicates complete adherence between neighboring cells and no vG in the lattice. The maximum intercellular air volume (beta, v/v), when alpha=0, was 0.593 for triangular, 0.2146 for square and 0,0931 for hexagonal lattices. The equation alpha=1- (vG/beta)½ was derived to relate alpha, beta and vG in the studied lattices. The relation (P S=p/alpha) between cell turgor (P S) and the tissue aggregating pressure (p), defined as the compression to keep in place a layer of cells, was demonstrated using the compression ratio concept....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elastic modules; Compression; External force; Intercellular air volume; Ipomea batatas; Pressure chamber.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100006

Plant population structure and insect herbivory on Solanum mauritianum Scopoli (Solanaceae) in southern Brazil: a support to biological control
Provedor de dados: 52
Autores: Barboza,Deise Mari; Marques,Márcia Cristina Mendes; Pedrosa-Macedo,José Henrique; Olckers,Terence.
Solanum mauritianum Scopoli (Solanaceae), a native Brazilian shrub, has become naturalized and invasive in several countries. In South Africa, where invasions are severe, herbivorous insects that attack S. mauritianum in its native area have been considered for introduction as biological control agents. To assess the action of such herbivores on the plant, studies were carried out on a population of S. mauritianum in an area undergoing regeneration in southern Brazil. An analysis of the structure of that population was performed, as well as of herbivory by insects, in particular of Anthonomus (Curculionidae). The population structure showed an "inverted J" pattern in diameter classes, but not in height classes. Individual plants showed an aggregate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population structure; Solanum; Anthonomus; Herbivory; Biological control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200019

Radionuclide imaging of the painful joint replacement: past, present and future
Provedor de dados: 52
Autores: Palestro,Christopher.
Differentiating aseptic loosening from infection as the cause of prosthetic joint failure is difficult because both entities are similar, clinically and histopathologically. Aseptic loosening frequently results from an immune reaction to the prosthesis. There is inflammation with an influx of histiocytes, giant cells, lymphocytes, and plasma cells. Proinflammatory cytokines and proteolytic enzymes are secreted, causing osteolysis and loosening. These same events occur in infection except that neutrophils, rarely present in aseptic loosening, are invariably present in infection. Clinical signs and symptoms, laboratory tests, x-rays and joint aspiration are insensitive, nonspecific or both. Artifacts produced by the metallic hardware hamper cross-sectional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radionuclide imaging; Painful joint replacement; Infection.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500003

Experimental bovine infection with Taenia saginata eggs: recovery rates and cysticerci location
Provedor de dados: 52
Autores: Minozzo,João Carlos; Gusso,Rubens L. Ferreira; Castro,Edilene A. de; Lago,Oscar; Soccol,Vanete Thomaz.
Studies were carried out on the recovery rate and cysticerci location in bovines experimentally infected with Taenia saginata eggs. Three calves of 6.5 months and one with 19 months of age were infected orally with 2 x 10(4) eggs of Taenia saginata. A fifth calf served as control. After 90 days of infection, the animals were slaughtered and organs and skeletal muscles were inspected using a slicing technique every 5 mm. From the four infected calves, 702 cysticerci were recovered, of which 570 (81.2%) were alive and 132 (18.8%) were degenerated. The recovery rate ranged from 0.01 to 1.43% with an average of 0.88%. The cysticerci presented the following anatomical distribution: hioideos muscles 02 (0.28%), kidneys 03 (0.43%), tongue 07 (1.00%), liver 12...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taenia saginata; Cysticercus bovis; Cysticerci location.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600008

Surimi of king weakfish (Macrodon ancylodon) wastes: texture gel evaluation with protease inhibitors and transglutaminase
Provedor de dados: 52
Autores: Kuhn,Cláudio Rafael; Prentice-Hernández,Carlos; Vendruscolo,João Luís; Soares,Germano Jorge Dorneles.
The protease inhibitors (bovine serum albumin -BSA - and egg white) and transglutaminase inhibitor (NH4Cl), was added to the surimi obtained by King weakfish (Macrodon ancylodon) wastes to evaluate your effect on the gel texture. Results indicated that the treatment with pre-heating (60°C, 30min + 90°C, 15min) favored the elasticity of the gel (ashi), demonstrating low proteolysis and characterizing the suwari phenomenon (high gel strength). Protease inhibitors increased gel strength significantly (P&lt;0.05) by compression force, when BSA was better than egg whites. Gels from surimi of King weakfish wastes didn't have action of the transglutaminase characterized.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Surimi; Protease inhibitors; Gel strength; Transglutaminase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600009

Effect of method of secondary fermentation and type of base wine on physico-chemical and sensory qualities of sparkling plum wine
Provedor de dados: 52
Autores: Joshi,Vinod K; Sharma,Sanjeev K; Goyal,Ravinder K; Thakur,Narayan S.
Plum base wines prepared with potassium metabisulphite or sodium benzoate were converted into sparkling wine, either by `Methode Champenoise' or tank method with artificially carbonated wine serving as a control. In both the secondary fermentation methods ethanol and low temperature acclimatized yeast; Saccharomyces cerevisiae UCD-595 with optimized sugar (1.5%) and di-ammonium hydrogen phosphate (0.2%) were used. Both methods of sparkling wine production and the type of base wine affected the physico-chemical and sensory characteristics of the sparkling wine produced. In the secondary fermented wines, most of the physico-chemical characteristics were altered compared to that of artificially carbonated wines except volatile acidity, methanol, propanol and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plum; Prunus salicina L.; Sparkling wine; Potassium metabisulphite; Sodium benzoate; Saccharomyces cerevisiae.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300008

Oral Administration of Citrus Extracts in Suckling Piglets
Provedor de dados: 52
Autores: Pereira,Lidiane Pescke; Hilgemberg,João Otávio; Primieri,Charlise; Mass,Anna Paula Holzmann; Lehnen,Cheila Roberta.
Abstract An experiment was conducted to evaluate the performance of suckling piglets subjected to oral administration of citrus extracts (CE). Twenty sows were used with ten sucking piglets on average initial weight of 1.26 kg (± 0.32) for 21 days. The experimental design was a randomized complete block design with two treatments: Negative Control (without antimicrobials) and Citrus Extracts (ascorbic acid + flavonoids, oral administration of 0.3 mg kg of BW via pigdoser) with ten repetitions each. The number of live-born piglets in females PO5 was 21% higher than in PO3 sows. Sows where piglets received CE showed a 10% higher (P &lt;0.05) feed intake in the last week of lactation. From 8 to 21 days, the feed conversion of NC sows was 23 and 38% lower (P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbic acid; Additives; Antibiotics flavonoids; Gut health.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100514

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil
Provedor de dados: 52
Autores: Minozzo,João Carlos; Moura,Juliana de; Almeida,Sérgio Monteiro; Thomaz-Soccol,Vanete.
Neurocysticercosis (NCC), the cerebral presence of Taenia solium metacestode (Cysticercus cellulosae), is responsible for neurological disorders worldwide. In order to validate an immunodiagnosis for public-health patients in the State of Parana-Brazil, crude antigen of Taenia crassicepsmetacestode (Cysticercus longicollis) was used as an alternative heterologous antigen to be used in ELISA and in electroimmunotransfer blotting (EITB) for active and inactive NCC diagnosis. Indirect ELISA was able to discriminate between active and inactive samples and presented high specificity and sensitivity. Any immunodominant band was able to distinguish the NCC stages, although the EITB showed 100% specificity. The immunological results proved to be an important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurocysticercosis; Immunodiagnosis; ELISA; EITB; Cysticercus longicollis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600007

Comparison between deep intracornual artificial insemination (dIAI) and conventional artificial insemination (AI) using low concentration of spermatozoa in beef cattle
Provedor de dados: 52
Autores: Meirelles,Ciro; Kozicki,Luiz Ernandes; Weiss,Romildo Romualdo; Segui,Marcio Saporski; Souza,Antonio; Santos,Ivo Walter dos; Breda,Jose Carlos dos Santos.
This study aimed to compare the pregnancy rate using the conventional artificial insemination (AI) or deep intracornual artificial insemination (DIAI), with low number of spermatozoa (4.0 million sperm) in 270 Nelore cows. The animals were divided in two groups (G: G1 (135 cows) conventional AI was performed (=semen deposition in the uterine body) and in G2 (135 cows) to DIAI, in ipsilateral horn where the dominant follicle in the ovary had previously been detected, by ultrasound examinations. For both the methods, a single artificial insemination was carried out after visual estrus observation, checked three times a day (morning, afternoon and evening). The pregnancy diagnosis after 45 days was conducted by ultrasound. Results showed a better pregnancy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deep intracornual artificial insemination; Beef cattle; Artificial insemination; Nelore cows.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300006

Identification and characterization of Fusarium mangiferae as pathogen of mango malformation in India
Provedor de dados: 52
Autores: Kumar,Pradeep; Kamle,Madhu; Misra,Asok Kumar; O'Donovan,Anthonia; Pagano,Marcela; Modi,Dinesh Raj.
ABSTRACT Fusarium mangiferae (=F. subglutinans) isolates collect from malformed samples from major mango-growing area of North India. Molecular identification and characterization of eleven most virulent isolates of F. mangiferae, based on pathogenicity tests used for the present study. Species-specific, genus specific ITS-PCR and PCR-RFLP performed for the accurate and easy detection of F. mangiferae. The rDNA-ITS 28S region sequences used for phylogenetic analysis of Fusarium isolates from India and other countries for homology search between them. The phylogenetic tree divided the isolates into three clades (i.e., American, Asian and African) and showed the high level of sequence based similarity (69-99%) among all Fusarium sequences from Asia. Thus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium mangiferae; Detection; Phylogenetic analysis; Vegetative malformation; Floral malformation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100204

Effect of Hydrostatic Pressure on the Fluorescence of Tryptophan in the Presence of Metal Ions Running title: Effect of pressure on the tryptophan
Provedor de dados: 52
Autores: Bao,Chengman; Tang,Xinhui; Ye,Shuming.
ABSTRACT The effect of pressure on the fluorescence of tryptophan in the presence of metal ions was studied by fluorescence spectrometry. It was found that at 60 MPa, the fluorescence intensity of M/Trp mixtures (M represented metal ions) increased compared to that at atmosphere pressure. The relative fluorescence efficiency of M/Trp mixtures increased with pressure. When the M/Trp ratio was above 10:1, the relative fluorescence efficiency in decreasing order was Cu2+/Trp mixtures, Ni2+/Trp mixtures and Mg2+ (K+)/Trp mixtures. When the ratio was below 10:1, the decreasing order was Cu2+/Trp mixtures and Ni2+ (Mg2+, K+)/Trp mixtures. The relative fluorescence efficiency increased with the concentration of Cu2+ and Ni2+. The variation was relate to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrostatic pressure; Tryptophan; Fluorescence; Metal ions.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100605

Lowering of phytic acid content by enhancement of phytase and acid phosphatase activities during sunflower germination
Provedor de dados: 52
Autores: Agostini,Juliana da Silva; Nogueira,Rosicler Balduíno; Ida,Elza Iouko.
The objective of this work was to investigate the germination of hybrid sunflowers BRS191 and C11 as a means of lowering phytic acid (PA) content by enhancing the activity of endogenous phytase and acid phosphatase. The concentration of PA in hybrid sunflower achenes varied from 2.16 to 2.83g/100g of sample (p &lt; 0.05). The phytase and acid phosphatase activities of sunflowers BRS191 and C11 were the highest on the 4th and 5th days of germination, respectively, with the release of the phosphorus. These results indicated that hybrid sunflower PA reduced and enhance phytase activity at distinct germination periods, which could open up the possibility of applying these enzymes in the control of PA content in cereals, thus improving their nutritional value.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germination; Sunflower hybrids; Phytic acid; Phytase; Acid phosphatase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400028

Evaluation of the use of vinasse as a biostimulation agent for the biodegradation of oily sludge in soil
Provedor de dados: 52
Autores: Crivelaro,Sérgio Henrique Rezende; Mariano,Adriano Pinto; Furlan,Luis Tadeu; Gonçalves,Renato Almeida; Seabra,Paulo Negrais; Angelis,Dejanira de Franceschi de.
This work aimed to study the effect of vinasse on the biodegradation in soil of oily sludge from the Replan-Petrobras oil refinery. The Bartha respirometric method was used to verify the efficiency of the treatments with soil, oily sludge (7 and 14% m/m) and soil moisture adjustment with or without vinasse (0.11 mL/g dry soil) for 121 days. Although an increase in the soil microbial population was obtained with the vinasse, it demonstrated not to be adequate to enhance the biodegradation efficiency of the oily sludge in soil, since there was no difference between the CO2 produced from treatments with or without vinasse after the complete consumption of the vinasse. Thus, the use of vinasse as an amendment to biodegradation processes showed not to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Soil; Oily sludge; Vinasse; Landfarming.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500027

Quality Index Method (QIM) to assess the freshness and shelf life of fish
Provedor de dados: 52
Autores: Bernardi,Daniella Cristina; Mársico,Eliane Teixeira; Freitas,Mônica Queiroz de.
The aim of this work was to develop a sensory method as the objective measure of quality of the fishes at all the key stages of fishery chain, from catch to consumer. The Quality Index Method (QIM) is based on a structured scaling for quality measurements and provides accurate and precise information concerning the freshness and a prediction of the remaining shelf-life for specie-specific fishes. The method is discussed and some future outlooks and need are pointed in order to stimulate the implementation of QIM in the relevant parts of the fishery chain giving unique information of the quality.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sensory analysis; Fish quality; QIM; Shelf life.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400009

Influence of NaNO3 concentration and incident light intensity on Nannochloropsis oculata lipid accumulation
Provedor de dados: 52
Autores: Gris,Lara Regina Soccol; Paim,Anderson de Campos; Farenzena,Marcelo; Trierweiler,Jorge Otávio.
This study aimed at evaluating the best conditions for lipid accumulation in the marine microalga Nannochloropsis oculata in an airlift photobioreactor. Experiments were carried out following a central composite design with the following variables: temperature (19 to 29°C), sodium nitrate concentration (NaNO3) in the culture medium (f/2) (25 to 125 mg.L-1) and incident light intensity (49 to 140 µE.m-2s-1). The maximum lipid production was 132.4 mg.L-1 under the following conditions: 27°C, NaNO3 concentration 105 mg.L-1 and 122 µE.m-2.s-1, which was 30% of dry weight of the biomass.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nannochloropsis oculata; Airlift Photobioreactor; Central Composite Design; Lipid.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400018

Purification and characterization of a beta-Glucanase produced by Trichoderma harzianum showing biocontrol potential
Provedor de dados: 52
Autores: Marco,Janice Lisboa de; Felix,Carlos Roberto.
A beta-1,3-glucanase was produced by Trichoderma harzianum in cultures containing chitin as the sole substrate. Two proteins showing beta-1,3-glucanase activity were purified to apparent homogeneity by hydrophobic chromatography. The molecular masses of these proteins were 29 and 36 kDa. The 36 kDa protein was further characterized. It was active on a broad pH range, and maximal activity was detected at pH 5.0. The optimum temperature of the 36 kDa beta-1,3-glucanase was 50ºC, but the purified enzyme was very sensitive to temperature. It lost about 60% or more of the activity after incubation for 30 min at 45, 50 and 60ºC. The apparent K M and Vmax for hydrolysis of laminarin at pH 5.0 and 37ºC, were 0.099 mg of reducing sugar/mL and 0.3 mg of reducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma harzianum beta-1; 3-glucanase chitinase proteases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100003

An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Giani,Tania Santos; Paoli,Severo de; Presta,Giuseppe Antonio; Maiworm,Adalgisa Ieda; Santos-Filho,Sebastião David; Brandão-Neto,José; Medeiros,Aldo da Cunha; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Red blood cell; Oxidant agent; Radiobiocomplex; Plasma proteins; Buzhong Yi Qi Wan.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600013

Dry Extract of Passiflora incarnata L. leaves as a Cardiac and Hepatic Oxidative Stress Protector in LDLr-/- Mice Fed High-Fat Diet
Provedor de dados: 52
Autores: Sarto,Danielle Aparecida Quintino Silva; Siqueira,Ana Helena D’Arcadia de; Magalhaes,Francisco Monteiro de Almeida; Caproni,Karine de Paula; Martins,Ângela Maria; Santos,Gérsika Bitencourt; Silva,Délcio Bueno da; Boas,Brígida Monteiro Vilas; Garcia,José Antônio Dias.
ABSTRACT The control of dyslipidemia by using herbal products is an important subject for studies. In this study, we evaluated the effects of dry Passiflora incarnata L. extract over dyslipidemia, left ventricular hypertrophy, and hepatic oxidative stress of LDL receptor knockout mice (LDLr-/-). Forty 4-month old male LDLr-/- mice were distributed into four groups: Group standard diet; Group standard diet and 200 mg/kg of body weight of Passiflora incarnata L. leaf dry extract; Group high-fat diet; Group high-fat diet and 200 mg/kg of body weight of Passiflora incarnata L. leaf dry extract. After 30 days, Passiflora incarnata L. dry extract reduced the effects of the high-fat diet, with a decrease of total cholesterol, triglycerides, and increase of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passion fruit; Left ventricular hypertrophy; Dyslipidemia; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100321

Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica)
Provedor de dados: 52
Autores: Sun,Zhen; Ma,Chunmei; Zhou,Jie; Zhu,Shuhua.
A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E. coli when the strains were grown for 4-5 h at 37 ºC. Highly pure protein was isolated by Ni2+-resin chromatography with 150 mM imidazole in 50 mM Tris, pH 8.0, by elution. In order to ensure that the recombinant SDH could be used for further study, the fluorescence and ultraviolet spectrum of the recombinant SDH were detected. Recombinant SDH was confirmed to be capable of oxidizing sorbitol by enzymatic activity assay. The activity of the recombinant SDH was 2.73 U mg-1min-1, which was similar with that directly extracted from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorbitol dehydrogenase; Recombinant SDH; Expression and purification; Ni-NTA; Enzyme assay; Peach fruit.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400002

Neural modeling of bromelain extraction by reversed micelles
Provedor de dados: 52
Autores: Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Tambourgi,Elias Basile.
A pulsed-cap microcolumn was used for bromelain extraction from pineapple juice by reversed micelles. The cationic micellar solution used BDBAC as the surfactant, isooctane as the solvent and hexanol as the co-solvent. In order to capture the dynamic behavior and the nonlinearities of the column, the operating conditions were modified in accordance with the central composite design for the experiment, using the ratio between the light phase flow rate and the total flow rate, and the time interval between pulses. The effects on the purification factor and on total protein yield were modeled via neural networks. The best topology was defined as 16-9-2, and the input layer was a moving window of the independent variables. The neural model successfully...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromelain; Reversed micelles; Extraction; Neural networks; Pineapple.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200026

Changes in amylase activity starch and sugars contents in mango fruits pulp cv. Tommy Atkins with spongy tissue
Provedor de dados: 52
Autores: Lima,Luiz Carlos de Oliveira; Chitarra,Adimilson Bosco; Chitarra,Maria Isabel F.
Changes in amylase activity, starch and reducing and non-reducing sugars contents were monitored during ripening of mango fruits (Mangifera indica L.). The climateric raising in mango fruit is marked by an appreciable increase in the activity of amylase, reducing and non-reducing sugars contents and decrease in the starch content. The fruit affected with spongy tissue exhibited much lower amylase activity and reducing and non-reducing sugars, but exhibited much higher starch content during storage at 12 ± 2° C and 90 ± 5% RH for 28 days, when compared to healthy tissue of ‘Tommy Atkins’. Whether this is caused due to adverse effects on certain enzyme activities during ripening is not clearly known. These dates showed that carbohydrate metabolism is an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Starch; Amylase; Reducing sugars; Non-reducing sugars.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100008

Investigation on Solar PV generation and design of switched reluctance motor for Smart Agriculture actuation system
Provedor de dados: 52
Autores: Muniraj,Murali; R.,Arulmozhiyal.
ABSTRACT This paper presents standalone solar photovoltaic (PV) powered fed actuation system employing a switched reluctance motor (SRM) particularly used in remote and rural areas. The converter efficiency is achieved by changing ON and OFF state of solar PV drive. An electronic commutation drives SRM drive with achieved by position hall sensor and encoder. The modified boost converter is proposed in single stage to conversion of PV fed power and inverter with reduced switching losses. Further proposed system is designed to reduce cost of system using simple design and control. This paper also proposes the speed control strategy of SRM motor with an artificial intelligent based Adaptive neuro fuzzy inference (ANFIS) system to achieve desired motor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SRM drive ANFIS Modified boost converter PV actuation MATLAB-SIMULINK SPEED GOAT.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100603

Seasonal dynamics and management of whitefly ( Bemesia tabaci Genn.) in tomato ( Solanum esculentum Mill.)
Provedor de dados: 52
Autores: Sharma,Devinder; Maqbool,Asifa; Jamwal,Vishav Vir Singh; Srivastava,Kuldeep; Sharma,Ashutosh.
ABSTRACT  Studies on seasonal dynamics of white fly (Bemesia tabaci) on tomato (Solanum esculentum var. Pusa Ruby) revealed that it appeared first during the 13th and reached maximum during 21st standard meteorological week. A positive correlation between adult population and abiotic factors viz. temperature (maximum and minimum) and sunshine hours was observed, whereas humidity (maximum and minimum) and rainfall showed a negative correlation with it. Taken together, the key weather parameters studied, caused 89.00 per cent variation in whitefly population (R2 value). Combination of carbofuran (soil application) + imidacloprid (seed treatment ) + imidacloprid (foliar application) proved significantly superior and caused maximum reduction in whitefly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tomato; Whitefly; Insect pest management.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100423

Gladius and statolith as tools for age and growth studies of the squid Loligo plei (Teuthida: Loliginidae) off southern Brazil
Provedor de dados: 52
Autores: Perez,José Angel Alvarez; Aguiar,Daniela Cordella de; Santos,João Antônio Teixeira dos.
Statolith and gladius were investigated for their suitability as tools for age and growth studies in the loliginid squid Loligo plei off southern Brazil. Statoliths when polished, revealed a series of concentric increments deposited around a nucleus. Consistent increment counts covered the squid's entire life-time and could be considered suitable for individual age estimation. Growth increments were observed on the dorsal surface as delimited by consecutive marks formed during low growth periods. Because early growth was masked during chitin deposition, total increment counts underestimated individual age. Gladius growth was highly correlated with somatic growth, and gladius increments could be used to reconstruct individual growth histories. Both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cephalopods; Statolith; Gladius; Age; Loligo plei; Growth.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600009

In vitro response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to 60Co at single fraction
Provedor de dados: 52
Autores: Andrade,Lídia Maria; Campos,Tarcisio Passos Ribeiro de; Leite,M. F.; Góes,A. M.
Radiotherapy using gamma rays is a common modality of breast cancer treatment. The aim of this research is to investigate the biological response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells (PBMC) exposed in vitro to 60 Co irradiation at a single fraction of 10 Gy, 25 Gy and 50 Gy doses at 136,4 cGy.min-1 rate. Cells were irradiated at room temperature by the Theratron 80 radiotherapy system. Biological response was evaluated through cellular viability using MTT assay and nucleus damages visualized by Propidium Iodide assay and electrophoresis agarose gel after gamma irradiation. Nucleus damages induced by 60Co irradiation were compared to damage caused by cell exposure to 10% methanol. The 50 Gy dose of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast Cancer; MDAMB-231; PBMC and Gamma rays.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700031

Colonization of benthic organisms on different artificial substratum in Ilha Grande bay, Rio de Janeiro, Brazil
Provedor de dados: 52
Autores: Azevedo,Flávia Beatriz Beserra; Carloni,Giuliano Guilherme; Carvalheira,Lucia Verçosa.
The aim of this work was to evaluate the influence of three different types of artificial substrates - concrete, metal and rubber - on the colonization of benthic organisms, using a structure called Multiple Disc Sampling Apparatus or MDSA. The results confirmed the hypothesis that the communities of incrusting invertebrates have preferences in relation to the type and orientation of the substratum to be colonized. Concrete and rubber, rougher surfaces, were more attractive to the organisms than the metal used. The orientation also had big influence because of the sedimentation that probably acted on the superior face of the materials, hindered the colonization.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial substrata; Colonization; Benthic organisms.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300012

Hydrogen Peroxide Induced Oxidative Damage on Mineral Density and Mechanical Properties of Bone
Provedor de dados: 52
Autores: Cicek,Ekrem; Cakmak,Enes.
ABSTRACT Hydrogen peroxide is a common reactive oxygen species involved in the catalytic mechanism though it is toxic to cells due to its oxidative nature. This work investigates the effects of hydrogen peroxide induced oxidative damage on bone mineral density and mechanical properties of bone which is primarily a composite material composed primarily of collagen fibers and biominerals. Sheep leg bones were exposed to hydrogen peroxide for a week. Bone mineral density was measured by using dual energy X-ray absorptiometry. Compressive modulus tests were applied to bone in order to determine mechanical properties. Our study shows that the hydrogen peroxide induced oxidative stress has negative effects on bone mineral density and stiffness. We observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone mineral density; Compressive modulus; Hydrogen peroxide; Reactive oxygen species.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100320

Effects of Arbuscular Mycorrhiza on Osmotic Adjustment and Photosynthetic Physiology of Maize Seedlings in Black Soils Region of Northeast China
Provedor de dados: 52
Autores: Xu,Hongwen; Lu,Yan; Zhu,Xiancan.
ABSTRACT To investigate the effect of arbuscular mycorrhiza fungi on maize growth, osmoregulation substances and photosynthetic physiology, a popular maize variety ZD 958 was measured under potted condition. Arbuscular mycorrhiza (AM) symbiosis promoted plant growth, and enhanced plant height, leaf length, mean leaf width and dry weight. Higher soluble sugar and protein, but lower proline concentrations were detected in AM seedlings than corresponding non-AM seedlings. Quantum yield of PSII photochemistry and potential photochemical efficiency increased by arbuscular mycorrhiza fungi, meanwhile, AM plants had lower primary fluorescence but higher maximal fluorescence and variable fluorescence than non-AM plants. AM enhanced apparent quantum efficiency,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arbuscular mycorrhiza; Maize; Osmotic adjustment; Light response curve.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100435

Ardra profiles of bacteria and archaea in mangrove sediments with different levels of contamination in the estuarine complex of Paranaguá, Brazil
Provedor de dados: 52
Autores: Ribeiro,Catherine Gérikas; Steffens,Maria Berenice Reynaud; Etto,Rafael Mazer; Galvão,Carolina Weigert; Martins,César de Castro; Pedrosa,Fábio de Oliveira; Kolm,Hedda Elisabeth.
The mangrove's sediments from the coastal areas under human activities may contain significant contaminations by hydrocarbons, even when there are no visual evidences of it. The microorganisms are essential to these ecosystems, especially in the control of their chemical environment. Sediment samples were collected in two regions under different environment conditions (pristine and contaminated) of the Paranaguá Estuarine Complex (Paranaguá Bay and Laranjeiras Bay), Brazil. Aliphatic hydrocarbons were determined by the GC-FID to assess the status of contamination of the studied areas. The total DNA was extracted from these samples. The 16S rRNA gene was amplified by the PCR reactions with the pair of primers 21F and 958R for the archaeal domain, and 27F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diversity; Mangroves; Archaea; Bacteria; 16S rRNA; ARDRA; Aliphatic hydrocarbons.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200013

N sources affect growth, nutrient content, and net photosynthesis in maté (Ilex paraguariensis St. Hil.)
Provedor de dados: 52
Autores: Gaiad,Sérgio; Rakocevic,Miroslava; Reissmann,Carlos Bruno.
The influence of different N sources on the growth of maté (Ilex paragurariensis St.Hil.) seedlings grown in greenhouse was studied. All seedlings received a base fertilization of 10 mg N.kg-1 soil as NH4NO3, 60 mg P2O5.and 40 mg K2O.kg-1 soil as KH2PO4 15 days before treatments application. Treatments were as follow: Control, with no extra N added; Urea = 100 mg N.kg-1 soil as Urea; NO3- = 100 mg N.kg-1 soil as Ca(NO3)2; and NH4+ = 100 mg N.kg-1 soil as (NH4)2SO4. It was concluded that: 1) increasing N content in leaves alone was not able to promote gain in biomass production of maté seedlings; 2) seedlings receiving N-NH4 showed a higher accumulation of P and Mg on shoot biomass; and 3) an increase in leaf area, leaf number and net photosynthesis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonium; Nitrate; Soil pH.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600001

Extraction of red cabbage anthocyanins: optimization of the operation conditions of the column process
Provedor de dados: 52
Autores: Xavier,Marcelo Fonseca; Lopes,Toni Jefferson; Quadri,Mara Gabriela Novy; Quadri,Marintho Bastos.
The aim of this work was to extract anthocyanins from the red cabbage. Batch studies under several extraction conditions indicated that acetic acid in aqueous solution (10% V/V) was the best solvent, used in the proportion of 0.25 g of red cabbage mL-1. At this condition, column assays were carried out to evaluate the influence of the ionic force, pH, solvent flow rate, recirculated volume of red cabbage juice and the mass of red cabbage. Results showed that the pH, recirculation and mass of red cabbage had statistically significant effects, where the optimum operation conditions found for the process were pH 2.3, recirculation volume of the solvent 0.83 L and mass of red cabbage 50 g.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthocyanins; Extraction; Red cabbage; Experimental design; Optimization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100018

Growth of the hermit crab Clibanarius vittatus (Bosc, 1802) (Crustacea, Anomura, Diogenidae) at São Vicente, São Paulo, Brazil
Provedor de dados: 52
Autores: Sant’Anna,Bruno Sampaio; Christofoletti,Ronaldo Adriano; Zangrande,Cilene Mariane; Reigada,Alvaro Luis Diogo.
The present study analyzed the growth of males and females of the hermit crab Clibanarius vittatus (Bosc, 1802), at São Vicente, São Paulo, Brazil. Monthly collections were made from May/2001 through April/2003, at the Pescadores Beach in São Vicente. The 2,501 crabs caught were identified and sexed and the carapace shield length (CSL) was measured. For the seasonal growth study, the population was divided into 5mm size classes (CSL) and analyzed by the Bertalanffy method, with the aid of the program Fisat II. The mean sizes of the 703 males and 1,798 females were 8.94±1.80 and 6.61±1.13 mm, respectively. A seasonal growth pattern was observed, with males reaching an asymptotic size (14.92 mm) larger than that of females (13.85 mm). Males began the growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Anomura; Clibanarius; Crustacea; Hermit crab.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300014

Viability of bull spermatozoa collected from the epididymis stored at 18-20°C
Provedor de dados: 52
Autores: Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Thomaz-Soccol,Vanete; Kozicki,Luiz Ernandes; Fujita,Aline Silva; Abreu,Renata Azevedo de; Green,Kerriel Thandile.
The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p&lt;0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bull; Epididymis; Orchiectomy; Sperm; Room temperature; Viability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008

Prostate cancer, treatment modalities and complications: an evaluation of the scientific literature
Provedor de dados: 52
Autores: Santos-Filho,Sebastião David; Missailids,Sotiris; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Prostate (PR) cancer (CA) is one of the most common malignant neoplasms in men all over the world. In general, if prostate cancer (PC) is detected early, treatment usually involves either surgical removal of the prostate or radiotherapy (RT). Hormone Therapy (HT) or chemotherapy (CH) is the preferred treatment for more advanced cases of PC or if CA spreads beyond the PT. A number of complications, such as urinary incontinence (IU) or erectile dysfunction (ED), can be associated with some modalities of treatment of the PC. The aim of this work is to evaluate, in PubMed, the number of publications related with prostate cancer and the main modalities of treatment, as well as some clinical complications. The searches were performed in PubMed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Radiotherapy; PubMed; Scientific interest.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700009

A presença da Galactoquinase em Tecidos Animais
Provedor de dados: 52
Autores: Bacila,Metry.
O autor relata trabalhos experimentais que o levaram a concluir pela existência da galactoquinase em tecidos animais, explicando desta maneira, que a primeira fase do metabolismo intermediário da galactose nos mesmos é a sua esterificação, em presença do ATP e da enzima em questão, ativada pelo magnésio, para galactose-1-fosfato.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galactokinase; Galactose metabolism; Animal tissues; Galactose-1-phosphate.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500012

Characterization of Rhizobacteria from field grown Genetically Modified (GM) and non-GM maizes
Provedor de dados: 52
Autores: Bumunang,Emmanuel Wihkochombom; Babalola,Olubukola Oluranti.
This study was done to examine the rhizobacteria from field grown Genetically Modified (GM) maize and its non-GM counterpart. Rhizospheric soil samples were collected at 30 days after sowing (DAS) and at post-harvest from two experimental fields in Gauteng, South Africa. Total rhizobacteria (cfu/g) in GM and non-GM soil samples was not significantly different across the different media 30 DAS and at post-harvest. Rhizobacterial isolates obtained were biochemically characterized using the analytical profile index. Species of Pseudomonas, Aeromonas, Sphingomonas, Burkholderia, Stenotrophomonas, Achromobacter, Ewingella and Bacillus were screened in vitro for plant growth promoting traits such as, ammonia production, catalase activity, indole acetic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetically modified maize; Plant growth promoting traits; Rhizosphere; Rhizobacteria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100001

Relationship between sugar cane cultivation and stream macroinvertebrate communities
Provedor de dados: 52
Autores: Corbi,Juliano José; Trivinho-Strixino,Susana.
The aim of this work was to study the influence of the sugar cane cultivation on the macroinvertebrate communities of 11 streams under different land uses. Analysis of the sediment (organic matter contents and metals) and water complemented the study. Ninety six macroinvertebrates taxa were identified, of which 54 were Chironomidae and 42 of other aquatic macroinvertebrates. The streams located in the areas of sugar cane culture and pasture presented low taxa and a great participation of Chironomidae (78% of the total). In contrast, the streams located in the areas with riparian vegetation presented a rich aquatic fauna with less participation of Chironomidae (60% of the total).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stream macroinvertebrates; Sugar cane; Land uses; Riparian vegetation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400015

Evaluation of the shelf-life of vegetable-type soybean pods
Provedor de dados: 52
Autores: Santana,Andréia Cristina; Carrao-Panizzi,Mercedes Concordia; Mandarino,José Marcos Gontijo; Leite,Rodrigo Santos; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko.
The shelf-life of the vegetable-type soybean pods stored under different conditions was evaluated by chemical characteristics and color. The pods were harvested in the R6 stage and stored either at 30 or 7ºC for 9 d. After the storage period, the pods were blanched and threshed, and the immature green grains were used for the analysis. The protein content decreased after 6 d of storage at 7ºC. There was no difference in the lipid content after the storage at 30 and 7ºC for 9 d. The starch and sucrose contents decreased after the first day of storage at 30ºC. There was no difference in trypsin inhibitor activity until 6 d of storage at 30 and 7ºC. The green color of the pods that was an indication of the quality that was maintained when stored at 7ºC during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetable-type soybean; Sucrose; Starch; Trypsin inhibitor; Color.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400015

Enzymatic Activity in the Gastrointestinal Tract of Pimelodus maculatus (Teleostei, Siluriformes) in Two Neotropical Reservoirs with Different Trophic Conditions
Provedor de dados: 52
Autores: Duarte,Silvana; Bemquerer,Marcelo; Araújo,Francisco Gerson.
Enzymatic activities for digestion of proteins and carbohydrates were compared among three organs of the digestive system of Pimelodus maculatus in two reservoirs with different trophic conditions during the winter of 2006. The aim was to test the hypothesis that enzymatic activity for the digestion of proteins and carbohydrates differed among organs and that such activities differ between the trophic state of the environment. Enzymatic activities were determined through the assays of specificity for trypsin, chymotrypsin and β-glucosidase enzymes. The intestine had higher trypsin-like enzymatic activities compared to the stomach and liver. The highest β-glucosidase activity was found in the liver compared to the stomach and intestine in the oligotrophic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Catfish; Physiology; Freshwater fishes; Digestive tract.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400605

Elimination of turbidity interference in serum iron colorimetric assay by enzymatic proteolysis
Provedor de dados: 52
Autores: Cardoso,Leonardo M.; Andrade,Milton H. G. de; Chianca Junior,Deoclécio A.; Silva,Marcelo Eustáquio; Pedrosa,Maria Lúcia.
We describe a modification in the commercial colorimetric method for the determination of serum iron by using Ferrozine® . The modification was proposed because during the conventional procedure, turbidity observed when the serum of animals submitted to surgery was used interfered with the assay. We added to the original method, a previous treatment of the serum with proteolytic enzymes. This modification was also tested using plasma samples, although this was not recommended when the original method was used. The results demonstrated that: a) the treatment with a mixture of trypsin and chymotrypsin was effective in order to eliminate turbidity; b) there was no difference between the standard curves obtained by the conventional and the modified method for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron assay; Enzymatic proteolysis; Interference.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300019

Evaluation of the performance of two strains of Nile tilapia (Oreochromis Niloticus) in mixed raising systems
Provedor de dados: 52
Autores: Neves,Patrícia Ribeiro; Ribeiro,Ricardo Pereira; Vargas,Lauro; Natali,Maria Raquel Marçal; Maehana,Káttia Regina; Marengoni,Nilton Garcia.
The aim of this study was to evaluate the productive performance of two strains of Nile tilapia (Oreochromis niloticus) in mixed raising systems. A total of 3600 fish-larvae species was used, 1800 belonging to Bouaké lineage, and 1800 to Chitralada. The experiment was carried out in three phases; Phase I in an incubator in 18 boxes, in which two treatments (Bouaké and Chitralada) were tested by using nine repetitions; Phases II and III were performed in 18 cement tanks with the same treatments. In phase I, regarding the final weight and gain of weight, Chitralada strain showed the highest final weight values. In phase II, Chitralada showed the highest final weight value when compared with Bouaké, and, considering the gain of weight, Bouaké obtained the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Performance; Strains; Oreochromis niloticus; Reproduction; Nile tilapia.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300012

Green Tropical Phytoextracts - Promising Anticancer Alternative
Provedor de dados: 52
Autores: Khan,Abid Ali; Omer,Kosar Ali; Talib,Ayesha; Ahmed,Haroon; Javed,Mohamad Arshad; Sarmidi,Roji Mohamad.
ABSTRACT The anticancer potential of aqueous extracts of tropical plants Ficus deltoidea and Labisia pumila were tested on human prostate carcinoma (DU145) cells. The effects of these extracts on the cells were observed through several tests such as cytotoxicity MTT assay, trypan blue exclusion assay (for cell viability), fluorescence and morphological observations. Both of the plant extracts had a growth repression effect on the cells with different concentrations. The best concentration (1x10-3 μg/mL) was chosen by determining their cytotoxic effects on human prostate cancer as well as fibroblast (normal) cells. The F. deltoidea extract showed more potency and killed the cells faster than L. pumila. Morphologically both extracts affected the cells in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Labisia pumila; Ficus deltoidea; Anticancer Plant Extract; Prostate cancer.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100328

Influence of tetracycline in the hepatic and renal development of rat's offspring
Provedor de dados: 52
Autores: Machado,Ana Lourdes da Silva; Brandão,Adriana Aigotti Haberbeck; Silva,Cláudia Maria de Oliveira Monteiro da; Rocha,Rosilene Fernandes da.
This study aimed on evaluating the possible effects of tetracycline administered to rats on the tenth day of pregnancy, on kidney and liver development of their offspring. The liver showed vacuolization, necrosis, inflammation and sinusoidal dilatations, more evident in the newborn. Mitosis, early increase of Kupffer cells population and hipertrophy of hepatocytes with greater synthesis of glycogen were present on the tenth and twentieth days of life. The kidney showed slight tubular vacuolizations and necrosis, more proeminent in the newborn, as well as signs of tubular regeneration on the tenth and twentieth days. These results suggested that the organs studied went through several transitory morphological changes during development but presented signs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pregnancy; Development; Tetracycline; Hepatotoxicity; Nephrotoxicity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100008

Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers
Provedor de dados: 52
Autores: Zeshan,Ali; Afzal,Muhammad; Alghamdi,Salem S.; Kettener,Karien; Ali,Mubashar; Mubushar,Muhammad; Ahmad,Shakeel.
ABSTRACT The presence of geneticdiversity is of great importance in improving wheat traits and developing strategies for optimal conservation of germplasm. Genetic diversity was assessed among common wheat cultivars using RAPD (Random Amplified Polymorphic DNA) markers at the Center of Agriculture, Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad. RAPD primers were used among 14 Pakistani wheat cultivars, to screen the progenies and for the identification of the genes of interest. The polymorphic information content (PIC), was measured as the percentage of polymorphic fragments for all primers. A total of 583 bands(84% polymorphic) in all 14 wheat cultivars was amplified and discriminated all the wheat genotypes. The number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; RAPD; Genetic diversity; PCoA; Cluster analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100432

Semi-volatile compounds variation among Brazilian populations of Ilex paraguariensis St. Hil.
Provedor de dados: 52
Autores: Cansian,Rogério Luis; Mossi,Altemir José; Mazutti,Márcio; Oliveira,José Vladimir; Paroul,Natalia; Dariva,Cláudio; Echeverrigaray,Sérgio.
The use of compressed carbon towards extracting semi-volatile compounds present in maté leaves (Ilex paraguariensis St. Hil.) is due to the growing interest in mate constituents to develop new products in the cosmetic, pharmaceutical and food industries. The objective of this work was to assess the chemical distribution of semi-volatile compounds in 20 native populations of maté collected all over Brazil. The extracts of bulk samples (30 plants) of each population were obtained by the high-pressure carbon dioxide extraction technique, and analyzed by GC/MSD. The quantification of compounds (caffeine, theobromine, phytol, squalene, vitamin E, eicosane, pentatriacontane, and stigmasterol) showed significant variations within the different populations and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maté; Chemical variability; Brazilian native populations; Supercritical extraction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100021

Temporal patterns in the occurrence of selected tropical fishes in mangrove creeks: implications for the fisheries management in north Brazil
Provedor de dados: 52
Autores: Giarrizzo,Tommaso; Krumme,Uwe.
To examine the temporal patterns in the occurrence of a tropical ichthyofauna, fisheries-independent samples were collected between September 2003 and July 2004 from intertidal mangrove creeks in the Curuçá estuary, Pará, north Brazil. Juveniles occurred year-round with the most intense occurrence during the wet/dry transition season (Anchovia clupeoides, Cetengraulis edentulus, Rhinosardinia amazonica, Mugil sp.). The occurrence of Colomesus psittacus and Anchoa hepsetus was continuous. Sciades herzbergii displayed two peaks (wet and dry season) while Cathorops sp. peaked only in the wet season. The continuous presence of juveniles in the tropical mangroves suggested that the fisheries management should be based on large no-take areas rather than closed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish recruitment; Juvenile fish; Mangrove estuary; Tropical fisheries management; Curuçá; Brazilian Amazon.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300020

Antineoplastic Effect of Procyanidin-rich Extract of Lafoensia Pacari in Lung Carcinoma Cells
Provedor de dados: 52
Autores: Cordeiro,Yonara de Gouveia; Rochetti,Arina Lázaro; Souza,Vinicius Castro; Silva,Edson Roberto da; Scatolini,Antônio Márcio; Genovese,Maria Inés; Yasui,George Shigueki; Fukumasu,Heidge.
Abstract Lafoensia pacari A. St. Hill has been used in traditional medicine as an anti-ulcerogenic and anti-inflammatory. Although there is an ethnopharmacological indication for cancer treatment, only a few studies have demonstrated its possible anticancer activity. Thus, the aims of this study were: (1) to evaluate the antineoplastic effect of L. pacari ethanolic extract (LPE) in lung carcinoma cells, (2) to determine the mode of action of LPE and (3) to identify the substances present in LPE. Human and murine lung cancer cell lines were grown in vitro and treated with different concentrations of LPE. Cell cycle and caspase-3 activity assays were performed in order to verify the mode of action. LC-ESI-MS screening was performed to detect the compounds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antineoplastic effect; Cell cytotoxicity; Medicinal plants; Procyanidins.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100302

Hydrosedimentological dynamic on Água Fria Watershed
Provedor de dados: 52
Autores: Silva,Alexandre Marco da; Schulz,Harry Edmar.
This study aimed surveying the amount of sediment yielded from the Água Fria watershed (Palmas, Tocantins, Brazil), from February-1998 to January-1999, and investigating the relations between the sediment yield and some environmental and/or antropic factors. The Colby's method was the technique employed for this investigation. The specific sediment yield and sediment delivery ratio were also determined for this period. It was estimated that 138,619 tons of sediment were yielded and the specific sediment yield for the study area was 827 t km-2 y-1, while the sediment delivery ratio was 6.2%. The suspended load was the most dominating fraction in almost all the studied period.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sediment yield; Sediment delivery ratio; Watershed.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500014

Temporal and spatial distribution of the ichthyofauna in two streams of the upper Rio Paraná basin
Provedor de dados: 52
Autores: Pavanelli,Carla Simone; Caramaschi,Érica Pellegrini.
The temporal and spatial distribution of the ichthyofauna was assessed in two streams, Caracu and São Pedro, affluents of the Rio Paraná, from March 1991 through February 1992. Samples were taken along the streams, using sieves (three sites on the Caracu and two on the São Pedro), and gill nets (two sites on the São Pedro). A total of 6,664 specimens belonging to 71 species were captured. CPE was calculated for each fishing gear. The data for cumulative frequency indicated that only the mouths of streams did not reach an asymptote. Constancy analysis showed that occasional and accessory species predominated. The spectrum of spatial diversity indicated that diversity increased toward the mouth of streams and temporal diversity increased in the high water...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community structure; Freshwater fishes; Stream ecology.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200019

Production and Optimization of Killer Toxin in Debaryomyces hansenii Strains
Provedor de dados: 52
Autores: Çorbacı,Cengiz; Uçar,Füsun Bahriye.
ABSTRACT Postharvest diseases of fruits and vegetables result in critical losses of production in worldwide. The losses often are caused by fungi and nowadays, most fungal pathogens are controlled by several strategies such as the use of fungicides. However, most of the fungicides are chemical-based compounds and are dangerous to human health and the nature. Therefore, the discovery of healthy and reliable strategies is crucial to control of fungal pathogens. In the paper, it was aimed to evaluate and characterize yeast isolates previously isolated from dairy products for the production of killer toxin. A total of 18 yeasts have been found to produce antagonistic behavior against susceptible fungal species. All of the yeasts expressing killer character...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Killer toxin; Optimization; Postharvest disease; Yeast.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100402

Produção de Lactobacillus plantarum em melaço de cana-de-açúcar
Provedor de dados: 52
Autores: Feltrin,Valdemar P.; Sant'Anna,Ernani S.; Porto,Anna C. S.; Torres,Regina C. O.
Comparative studies of Lactobacillus plantarum were carried out in MRS broth (control) and in 3% (w/v) sugar cane blackstrap molasses enriched as follows: 0.5% yeast extract, 0.5% ammonium citrate and 0.5% sodium acetate (Medium 1) and 0.5% yeast extract, 0.5% ammonium citrate, 0.5% sodium acetate, 0.5% ammonium phosphate, 0.2% ammonium citrate, 0.1% Tween 80 and 0.005% manganese sulphate (Medium 2). The experiment, was carried out at a 5 L fermentor, with 3.5 L as working volume. At the end of the fermentation period MRS presented the highest viable cells production (28.68 log CFU/mL) while in Medium 2 it was 25.80 and in Medium 1 19.36 log CFU/mL. Biomass productivity was 0.0865 g.L-1.h for MRS (control), 0.0768 and 0.0507 g.L-1.h for Medium 2 and Medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus plantarum; Fermentation; Molasses.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100015

Flour mixture of rice flour, corn and cassava starch in the production of gluten-free white bread
Provedor de dados: 52
Autores: López,Ana Cristina Ballesteros; Pereira,Accacia Julia Guimarães; Junqueira,Roberto Gonçalves.
The use of rice flour corn and cassava starch was evaluated in several formulations aiming to find a flour mixture to replace wheat flour in the production of free-gluten white bread. Production parameters were evaluated through sensory analysis. The resulting breads were evaluated taking into account physical parameters (crumb appearance, specific volume and moisture) and sensorial parameters (flavor, appearance, crumb texture, crust color and satisfaction). Regarding flavor and moisture, breads prepared with the three different ingredients were not statistically different at 5% probability by the Tuckey test. However, they differed significantly regarding the specific volume, crumb texture, crust color, degree of satisfaction and external appearance....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celiac disease; Gluten-free bread; Corn starch; Cassava starch; Rice flour.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100009

TT virus in peripheral blood cells from patients with human papillomavirus (HPV): investigating association with cervical carcinoma
Provedor de dados: 52
Autores: Suzuki,Patrícia Sayuri; Oliveira,Karen Brajão de; Herrera,Ana Cristina do Amaral; Borelli,Sueli Donizete; Guembarovski,Roberta Losi; Oliveira,Carlos Eduardo Coral de; Oda,Julie Massayo Maeda; Ozawa,Patricia Midori Murobushi; Watanabe,Maria Angelica Ehara.
Torque Teno Virus (TTV) presence was investigated in peripheral blood of 117 brazilian women by nested polymerase chain reaction. TTV DNA was observed in 18.6% of healthy donors and in 24.32% Human Papillomavirus (HPV) patients. TTV presence was also investigated in the HPV positive group for comparison between the cervical cancer and noncancerous patients. TTV DNA prevalence was significantly higher among the HPV positive patients with cervical cancer (57.14%) than in HPV noncancerous patients (16.67%). Thus, the presence of TTV infection could be a risk factor for cancer development in the patients presenting HPV-TTV coinfection. Further studies are required to clarify the TTV influence in HPV pathogenesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TTV DNA; HPV; Cervical cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200010

Safety Assessment of a New Pigmented Safflower Seed Coat (A82) by a Feeding Study on Rat
Provedor de dados: 52
Autores: Karami,Soraya; Sabzalian,Mohammad R.; Khorsandi,Layasadat; Rahimmalek,Mehdi.
ABSTRACT Safflower (Carthamus tinctorius L.) is an annual herbaceous plant, cultivated mainly for the seed which is used for edible oil extraction and bird feeding. This study was designed to evaluate the safety of a new pigmented variety of safflower (A82) seeds. The results showed that oral administration of A82 seeds significantly increased the body weight of male rats in a dose-dependent manner (p&lt;0.05). Biochemical tests showed that A82 seeds significantly increased the serum levels of AST (Aspartate aminotransferase) (p&lt;0.05), slightly reduced the serum levels of ALT (Alanine aminotransferase) and significantly reduced ALP (p&lt;0.05) levels in a dose dependent manner. BUN (Blood Urea Nitrogen) and Cr (Creatinine) were not significantly changed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Safflower black seed; Herbal medicine; Food safety; Toxicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100503

Nephrotoxicity of Bence-Jones proteins: correlation with endocytosis by BHK cells and intracellular movement
Provedor de dados: 52
Autores: Nicastri,Ana Lucia; Gomes,Elisa Maines; Prado,Maria José Brandão de Almeida; Prado,Euthymia Brandão de Almeida.
The aim of this investigation was to evaluate the endocytosis of two Bence-Jones proteins by renal cells in order to elucidate the interference of their physical and chemical characteristics on nephrotoxicity. Bence-Jones proteins (AK and GL) were purified and isolated from the urine of two patients with multiple myeloma. The isotype of both proteins was characterised as being human monoclonal lambda light chain. The AK protein presented mainly an Ip&gt;7.0, a high content of galactose and a low amount of sialic acid molecules. On the other hand, the GL protein presented a single band with an Ip of 4.3, a higher level of sialic acid and a reduced amount of galactose, in comparison with the AK protein. Baby Hamster Kidney (BHK) cells were maintained in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocytosis; Bence-Jones; Experimental Nephrology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200009

Contribuição ao conhecimento da incidência da brucelose no Estado do Paraná (Brasil)
Provedor de dados: 52
Autores: Palmquist,Oscar K.
O rebanho bovino paranaense apresenta um índice de brucelose relativamente alto, particularmente no Norte e Leste do Estado. O gado leiteiro revelou um índice de 7,90%, com um foco grave no Município de Castro (29,92%). Bovinos criados em regime extensivo de pastagens naturais se apresentam com menor grau de infecção, pois a maior incidência foi encontrada no Norte do Estado, onde, regra geral, os campos são constituídos de pastos artificiais.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucellosis; State of Paraná; Cow’s milk.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500014

FDOPA-(18F): a PET radiopharmaceutical recently registered for diagnostic use in countries of the European Union
Provedor de dados: 52
Autores: Chevalme,Yanna-Marina; Montravers,Françoise; Vuillez,Jean-Philippe; Zanca,Michel; Fallais,Charles; Oustrin,Jean; Talbot,Jean-Noël.
Positron emission tomography (PET) and its recent update PET/CT are very effective diagnostic tools for non-invasive imaging of metabolic or functional disorders in target tissues. The clinical usefulness of fluorodeoxyglucose-(18F) (FDG) has been now widely accepted. Recently, the clinical usefulness of fluoroDOPA-(18F) or FDOPA, an aminoacid labelled with the same positron emitter fluorine-18, has been evaluated and recognised in France and subsequently in several EU countries. FDOPA is diagnostic PET agent, which has been used for decades in imaging the loss of dopaminergic neurons in Parkinson's disease, and more recently to detect, stage and restage neuroendocrine tumours and to search for recurrence of viable glioma tissue. The present article...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Registration of radiopharmaceuticals; PET; FDOPA; Parkinson's disease; Cancer.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600009

Calculation of fermentation parameters from the results of a fed-batch test taking account of the volume of biomass in the fermenting medium
Provedor de dados: 52
Autores: Borzani,Walter.
The volume of biomass in the fermenting medium may significantly affect the values of fermentation parameters calculated from the measured concentrations of the substrates and/or products. The corrections proposed in this paper should be evaluated and, depending on their magnitude, considered in order to obtain more representative results. A numerical example is presented.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass volume; Corrections calculation; Fed-batch fermentation; Fermentation parameters; Medium aqueous phase volume.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300001

Development of a gas chamber for detecting broiler chicken halothane sensitivity and PSE (Pale, Soft, Exudative) meat formation
Provedor de dados: 52
Autores: Marchi,Denis Fabrício; Oba,Alexandre; Ziober,Iris Lamberti; Soares,Adriana Lourenço; Ida,Elza Iouko; Shimokomaki,Massami.
The objective of this work was to investigate the use of a halothane to screen broiler chickens prone to develop PSE (Pale, Soft, Exudative) meat through a special gas chamber connected to a veterinarian anaesthetic apparatus developed in our laboratory. Anaesthesia was induced with approx. 3.0% halothane at a flow rate of 6.0 Lm-1 in pure oxygen for 5 min. Commercial male broilers (n=342) aged 46 days old were evaluated and classified as either halothane sensitive (HAL+) or insensitive (HAL-), depending on the leg rigidity response. Approximately 27.5% (n=94) of broilers were HAL+ and 72.5% (n=248) were HAL-. This is a simple and rapid technique to evaluate broiler sensitivity to halothane and identify broilers prone to develop PSE meat. The occurrence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Halothane test; Colour; PH.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700024

Larval predation on different instars in blowfly populations
Provedor de dados: 52
Autores: Faria,Lucas Del Bianco; Godoy,Wesley Augusto Conde; Reis,Sérgio Furtado dos.
During its larval stage, Chrysomya albiceps (Diptera: Calliphoridae) is a facultative predator on other blowflies. In this study, we evaluated the predation by third instar larvae of C. albiceps on first, second and third instar larvae of Chrysomya megacephala and Cochliomyia macellaria in no-choice experiments in order to compare the vulnerability of larval instars to predation. With first and second instar prey the highest predation rate by C. albiceps was on C. megacephala. For third instar prey, the highest predation rate was on C. macellaria. With second instar prey, there was complete predation on C. megacephala within 90 min, whereas in C. macellaria only 55% of the larvae were eaten by 90 min. For third instar prey most predation on C. macellaria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chrysomya albiceps; Blowflies; Larval predation; Optimal foraging.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600008

Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
Provedor de dados: 52
Autores: Stergiou,Panagiota-Yiolanda; Foukis,Athanasios; Theodorou,Leonidas; Papagianni,Maria; Papamichael,Emmanuel.
The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular enzymes; Α-amylase; Kluyveromyces marxianus; Fermentation; RSM.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016

Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077
Provedor de dados: 52
Autores: Gowda,Vaishnavi; Shivakumar,Srividya.
The study identified the innate enzymatic potential (amylase) of the PHB producing strain B.thuringiensis IAM 12077 and explored the same for cost-effective production of PHB using agrowastes, eliminating the need for pretreatment (acid hydrolysis and/or commercial enzyme). Comparative polyhydroxyalkanoate (PHA) production by B. thuringiensis IAM 12077 in biphasic growth conditions using glucose and starch showed appreciable levels of growth (5.7 and 6.8 g/L) and PHA production (58.5 and 41.5%) with a PHA yield of 3.3 and 2.8 g/L, respectively. Nitrogen deficiency supported maximum PHA yield (2.46 g/L) and accumulation (53.3%). Maximum growth (3.6 g/L), PHB yield (2.6 g/L) and PHA accumulation (72.8%) was obtained with C:N ratio of 8:1 using starch as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis IAM 12077; Polyhydroxybutyrate; Nutrient limitation; Hydrolytic enzymes; Mango peel.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100009

Larval development of hoplias cf. Lacerdae (Pisces: Erythrinidae) and delayed initial feeding effects
Provedor de dados: 52
Autores: Sirol,Rodolfo N.; Menin,Eliane; Andrade,Dálcio R.; Silva,Martinho A.
Larval development of Hoplias cf. lacerdae was studied under laboratory conditions. After hatching, ontogenetic changes were recorded on food-deprived larvae in 12-hour intervals. Mouth opening occurred after 2.5 days and notochord terminated flexure in 6.5 days. Notochord length increased at a constant rate until complete yolk absorption (13,5 days). Larval dry weight and body height diminished gradually up to 21 days after hatching, when all starved larvae died. Every 12 hours after yolk absorption, groups of larvae (n=15), were separeted, and fed with Artemia nauplii for 10 days. The point-of-no-return (when 50% of larvae were unable to feed or to assimilate ingested food after delayed feeding), was not apparent in this species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish larva; Hoplias; Larval development; Starvation; Point-of-no-return.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100006

Biological and chemical control of Sclerotinia sclerotiorum using Trichoderma spp. and Ulocladium atrum and pathogenicity to bean plants
Provedor de dados: 52
Autores: Figueirêdo,Girlene Soares de; Figueirêdo,Lívio Carvalho de; Cavalcanti,Francinete Carla Nunes; Santos,Angela Coimbra dos; Costa,Antonio Felix da; Oliveira,Neiva Tinti de.
Four isolates of Sclerotinia sclerotiorum were tested for pathogenicity in IPA-10 variety bean plants (Phaseolus vulgaris L.), and all were pathogenic. Biological control in vitro was evaluated using eight isolates of Trichoderma spp. and, one of Ulocladium atrum. Chemical control in vitro with fungicides Thiophanate methyl, Iprodione and Carbendazim was also tested. Except U. atrum, all Trichoderma isolates showed antagonistic potential against S. sclerotiorum, where isolate 3601 presented the best performance. Thiophanate methyl chemical control was the most efficient. This fungicide and isolate 3601were compared in vivo in greenhouse. There was statistical difference between the treatments, and the application of fungicide and antagonist before the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S. sclerotiorum; Trichoderma; Antagonist; Bean plant.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100001

Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity
Provedor de dados: 52
Autores: Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de.
The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003

Performance Analysis of Small Grid Connected Photovoltaic Systems
Provedor de dados: 52
Autores: Hioki,André Tsuyoshi; Silva,Vilson Roiz Gonçalves Rebelo da; Vilela Junior,João Américo; Loures,Eduardo de Freitas Rocha.
Abstract The acceptance and deployment of electric power from sustainable sources, which are less polluting than fossils, have been a consensus throughout society. Specially, the growth of the installed capacity in photovoltaic energy has been considerable in distributed generation. In order to properly take advantage of this growing demand, it is necessary to adopt appropriate measures and procedures to improve the operation and performance of photovoltaic systems. The purpose of this work is to present such measures and procedures, under quantitative and qualitative analysis, using scientific methodologies and tools. In the end, a set of procedures was obtained that analyzes functional and structural aspects of small grid-connected photovoltaic systems....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distributed generation; Performance; Small photovoltaic system.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200217

Biological properties of Endophytic Fungi
Provedor de dados: 52
Autores: Sudha,Venkatesan; Govindaraj,Ramar; Baskar,Kathirvelu; Al-Dhabi,Naif Abdullah; Duraipandiyan,Veeramuthu.
The endophytic fungus is have rich source of secondary metabolites which act as biological active agent in the higher plants. The fungal derivatives play vital part in human life and their compounds are the source of drug for cancer, microbial and viral diseases. The natural compounds from the endophytes acts as growth inhibitor of plant pathogenic organism. Endophytes are rich sources of natural products which are used in agriculture (plant growth and insecticidal), pharmaceutical industries and also used for phytoremediation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic fungi; Secondary metabolites; Anticancer; Phytoremediation; Pest management.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100413

Modeling gallic acid production rate by empirical and statistical analysis
Provedor de dados: 52
Autores: Kar,Bratati; Banerjee,Rintu; Bhattacharyya,Bimal Chandra.
For predicting the rate of enzymatic reaction empirical correlation based on the experimental results obtained under various operating conditions have been developed. Models represent both the activation as well as deactivation conditions of enzymatic hydrolysis and the results have been analyzed by analysis of variance (ANOVA). The tannase activity was found maximum at incubation time 5 min, reaction temperature 40ºC, pH 4.0, initial enzyme concentration 0.12 v/v, initial substrate concentration 0.42 mg/ml, ionic strength 0.2 M and under these optimal conditions, the maximum rate of gallic acid production was 33.49 mumoles/ml/min.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallic acid; Modeling; Tannase; Caesalpinia digyna.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500010

Tanneries: from waste to sustainability
Provedor de dados: 52
Autores: Farenzena,Marcelo; Ferreira,Luciane da Silveira; Trierweiler,Jorge Otávio; Aquim,Patrice Monteiro de.
The aim of this work was to look for an efficient and environmentally safe solution for the leather tanning industry through a problem of non-linear programming. The results were promising and showed a significant potential of wastewater reduction. Reuse of internal streams reduced around 70% wastewater. The solutions obtained could reduce significantly the amount of chemicals and consequently the amount of waste to be treated.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water reuse; Massic integration; Wastewater reduction; Superstructure; Tanneries.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400035

Tolerância à inundação: aspectos da anatomia ecológica e do desenvolvimento de Sesbania virgata (Cav.) Pers. (Fabaceae)
Provedor de dados: 52
Autores: Davanso-Fabro,Viviane M.; Medri,Moacyr E.; Bianchini,Edmilson; Pimenta,José A.
Plantas de Sebania virgata (Cav.) Pers. (Fabaceae) cultivadas em casa de vegetação foram alagadas por 40 dias. Plantas alagadas apresentaram aumento do peso da matéria seca e comprimento, acentuadas rachaduras corticais e intumescimento cortical esponjoso em caules e raízes, raízes superficiais e raízes adventícias. É possível que a plasticidade morfo-anatômica apresentada por esta espécie esteja contribuindo para o seu estabelecimento em solos hipóxicos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sesbania virgata; Hipoxia; Anatomia Ecológica.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400012

Influence of thickness on properties of plasticized oat starch films
Provedor de dados: 52
Autores: Galdeano,Melicia Cintia; Wilhelm,Allan Eduardo; Mali,Suzana; Grossmann,Maria Victória Eiras.
The aim of this study was to investigate the effect of thickness (between 80 and 120 µm) on apparent opacity, water vapor permeability and mechanical properties (tensile and puncture) of oat starch films plasticized with glycerol, sorbitol, glycerol:sorbitol mixture, urea and sucrose. Films were stored under 11, 57, 76 and 90% relative humidity (RH) to study the mechanical properties. It was observed that the higher the thickness, the higher was the opacity values. Films without the plasticizer were more opaque in comparison with the plasticized ones. Glycerol:sorbitol films presented increased elongation with increasing thickness at all RH. Puncture force showed a strong dependence on the film thickness, except for the films plasticized with sucrose. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasticizers; Tensile; Puncture; Permeability; Opacity; Glycerol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400014

Modeling for Taxol® Separation in a simulated moving bed
Provedor de dados: 52
Autores: Cremasco,Marco Aurelio; Starqui,Axel Nicolas.
This work presents an alternative numerical resolution strategy for a model to describe the dynamic of linear adsorption processes involving multicomponent mixture of taxanes with Taxol® (paclitaxel), a powerful anti-cancer agent, and non-identified impurities, in a Simulated Moving Bed (SMB) system. To solve the model, a hybrid method were used. The liquid concentration inside the particles was found analytically and was related with the liquid bed concentration using Duhamel's theorem. The results from simulation were compared with experimental ones from the literature, showing a good agreement, which demonstrated the applicability of the model and of the hybrid resolution proposed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Taxol; Simulated moving bed; Computer simulation; Multicomponent separation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600020

Editorial
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500001

Beer Clarification with polysulfone membrane and study on fouling mechanism
Provedor de dados: 52
Autores: Oliveira,Ricardo Cardoso de; Barros,Sueli Teresa Davantel de.
The aims of the present work were to study the relationship between the fluxes, permeate quality, and fouling mechanism. A polysulfone membrane with 100 KDa and 0.12 m² of surface area was used. Permeate fluxes were measured for different pressures (0.5, 1.0, 1.5, and 2.0 bar) at the same temperature of 8 ºC. The fluxes measured for each pressure ranged from 22, 24, 27 and 30 kg h-1m-2 at 0.5, 1.0, 1.5 and 2.0 bar, respectively. Samples of the feed and permeate were analyzed for pH, color, turbidity, sugar, bitterness, and proteins. The fouling mechanisms observed were cake filtration, partial pore blocking, and complete pore blocking.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microfiltration; Polymer Membrane; Pore Blocking; Turbidity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600020

Determination of Diffused Irradiation from Horizontal Global Irradiation - Study for the City of Curitiba
Provedor de dados: 52
Autores: Souza,Muriele Bester de; Tonolo,Édwin Augusto; Yang,Renata Lautert; Tiepolo,Gerson Máximo; Urbanetz Junior,Jair.
Abstract Understanding the solar radiation values ​​in the surface is important for the development of solar energy projects, obtaining through radiometers installed in certain places or publications as in solar energy atlases. Typically, solar or weather stations do not have sensors to measure diffuse irradiation due to high investment and the need for constant maintenance. This paper presents methods for the determination of diffuse irradiation from surface measured data of global horizontal irradiation in the city of Curitiba, obtained through pyranometers installed at the Federal University of Technology – Paraná (UTFPR) headquarters and from Curitiba-A807 National Institute of Meteorology (INMET) station. Two models are presented for the estimation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diffuse Irradiation; Estimate; Global irradiation; Solar energy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200213

A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol
Provedor de dados: 52
Autores: Anschau,Andréia; Santos,Lucielen Oliveira dos; Alegre,Ranulfo Monte.
This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione; Fermentation; Fed Batch Culture; Amino Acids; Response Surface Methodology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500017

Targeted tumor radiotherapy
Provedor de dados: 52
Autores: Unak,Perihan.
Targeted tumor radiotherapy is selectively delivery of curative doses of radiation to malignant sites. The aim of the targeted tumor radiotherapy is to use the radionuclides which have high LET particle emissions conjugated to appropriate carrier molecules. The radionuclides are selectively collected by tumor cells, depositing lethal doses to tumor cells while no admission occur to normal cells. In theory, targeted radiotherapy has several advantages over conventional radiotherapy since it allows a high radiation dose to be administered without causing normal tissue toxicity, although there are some limitations in the availability of appropriate targeting agents and in the calculations of administered doses. Therefore, for routine clinical applications...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Targeted Tumor Radiotherapy; Radionuclide cancer therapy; High LET radiation; Auger electron radiotherapy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500014

Modeling of drying and adsorption isotherms of the fish feed
Provedor de dados: 52
Autores: Pacheco,Ana Claudia Werner; Luz,Gianini Regina; Polon,Paulo Eduardo; Jorge,Luiz Mário de Matos; Paraíso,Paulo Roberto.
The aim of this work was to study the drying curves and equilibrium isotherms of extruded fish feed. The drying curves were determined at air temperatures of 50, 60, 70 and 80°C and airflow velocities of 1.5, 2.5 and 3.5 m/s). The equilibrium isotherms of relative humidity of air were obtained between 10 and 80% at 30, 40, 50, and 70°C. The experimental data were fitted for non-linear regression by using Statistica® to the models reported in the literature. The results showed that the drying curves and the equilibrium isotherms were significantly influenced by variations of the air temperature in a similar way to solid materials as described in the literature. The statistical results for models of Page and Peleg showed that the fitting of the experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extruded fish feed; Adsorption isotherms; Equilibrium moisture.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300020

Structure of molluscan assemblages in sheltered intertidal unconsolidated environments
Provedor de dados: 52
Autores: Denadai,Márcia Regina; Amaral,Antonia Cecilia Zacagnini; Turra,Alexander.
The molluscan macrofauna from 13 oceanic sheltered intertidal unconsolidated environments and its relationship with abiotic factors were studied in order to establish the degree of species richness and to understand the role environment plays in structuring such assemblages. Four distinct intertidal habitat types were recognized based on molluscan assemblage descriptors (diversity, richness and density) and abiotic characteristics. The mean grain size (in phy units) and the beach slope showed a negative relationship with the diversity, richness and density. Coarser sediments were favorable to molluscan fauna in the study areas, contrasting the well-known negative effect of this type of sand on fauna in typical oceanic beaches. The low-tide terraces,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intertidal; Molluscan assemblage; Beach structure; Environmental heterogeneity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600019

Soybean parent selection based on genetic diversity
Provedor de dados: 52
Autores: Carpentieri-Pípolo,Valéria; Pípolo,Antônio Eduardo; Silva,Flávio André Martins da; Petek,Marcos Rafael.
Thirty-four soybean lines were assessed for twelve traits. The genetic distances were estimates using multivariate techniques, to identify parents to be included in breeding programs for hybridization. Grouping by the Tocher method, from generalized Mahalanobis distances, divided the 34 lines into four groups. The most important agronomic traits, weight of seeds per plot, plant height, height of first pod and days to maturity were considered when recommending for crossing. The following crosses were recommended based on the genetic divergence and the key agronomic traits: lines 23, 10, 2, 27 and 25 (group I) with genotype 6 (group II) and genotype 16 (group III). Thus only ten crosses would be made, representing only 2% of the total crosses which could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Genetic divergence; Mahalanobis; Multivariate analysis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300008

Plant reproductive phenology and dispersal patterns after natural regeneration in a limestone mining spoil banks
Provedor de dados: 52
Autores: Frenedozo,Rita de Cássia.
Studies were carried out on the phenological patterns and dispersion of a plant community developed naturally after limestone mining operations, in accumulated materials of wastes (spoil banks). The objectives were to focus the seasonality of plant reproductive in a degraded environment; to analyze the community according to dispersal syndrome and to know about plant resources for the animals Monthly observations of flowering and fruiting patterns of the community were done, comparing the herbaceous, shrubs and woody species. Proportion of anemochorous and autochorous was higher in the herbaceous and shrubby strata. The zoochorous ones, on the contrary, were more frequent in the woody stratum. This study showed that natural introduction of the animals at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenology; Mining; Brazil; Plant dispersal; Herbivores; Community.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200014

Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants
Provedor de dados: 52
Autores: Nunes,Maria Paula; Souza-Paccola,Ednéia Aparecida de; Andrade-Nóbrega,Gisele Maria; Paccola-Meirelles,Luzia Doretto.
The aim of this study was to assess oval conidia production in mutants for the reductase nitrate gene in C. sublineolum and verify the possibility of using them as a tool to transfer the genetic material in this species. The mutants used in the present study lost the ability to form falcate conidia but all produced oval conidia. The number of nuclei by conidium was evaluated. Oval conidia were efficient in the heterokaryon formation and these heterokaryons in liquid culture medium they also produced oval conidia in abundance. Recombinants were obtained and the genetic exchanges were confirmed by the RAPD analyses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic recombination; Nit mutants; Parameiosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100021

Safety of Beauveria bassiana (Bals.) Vuill. to Gallus domesticus L.
Provedor de dados: 52
Autores: Haas-Costa,Jucelaine; Alves,Luis Francisco Angeli; Daros,Alaxsandra Aparecida.
Beauveria bassiana, an important insect pathogen was used to evaluate its effect on the feeding, behavior, histology of the digestory system and anatomy of Gallus domesticus. The fungus (strain Unioeste 4) was administrated orally treated with chicken food. Chicken feces were collected, and the birds observed for 28 days. Also, were evaluated the weight of the birds, as well as any sign of intoxication or pathological modification. Tissue samples were withdrawn to test lesions with the optic microscope. There was 100% of survival of the birds, and no behavior alteration or external lesion was found. The test group presented the highest weight gain (1383.9±54.4g). Viable conidia were observed in the feces until 24 h after feeding the fungus, indicating that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Microbial control; Non-target organisms; Birds.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200027

An experimental evaluation of the effectiveness of beach ashtrays in preventing marine contamination
Provedor de dados: 52
Autores: Widmer,Walter Martin; Reis,Rodrigo Arantes.
In this study, hypotheses concerning the use of beach ashtrays were experimentally tested. Results indicated that the mean rate of abandonment of this equipment was low (1.5%). The mean amount of cigarette stubs (3.4 items/ashtray) was greater than mean amounts of other types of litter. People with different socioeconomic profiles had different perceptions regarding the issues associated with beach debris. These results indicated that beach ashtrays could be useful to prevent the contamination of these environments and that differences in socioeconomic characteristics of beachgoers could partially explain the differences in perceptions regarding the presence of waste on the beaches. This information could now be used by coastal managers to plan strategies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beach; Brazil; Cigarette butts; Coastal management; Marine debris; Paraná.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500026

The use of radionuclide DNA probe technology for epidemiological studies of tegumentary leishmaniasis in Mato Grosso state
Provedor de dados: 52
Autores: Andrade,Antero Silva Ribeiro de; Fernandes,Octávio; Heub,Marcia; Carvalho,Maria de Lourdes Ribeiro; Fontes,Cor Jesus; Melo,Maria Norma de.
DNA hybridisation, using probes labelled with 32P, was used to type Leishmania samples isolated from patients living in endemic areas of Mato Grosso State (Brazil), and clinically diagnosed as having tegumentary leishmaniasis. kDNA cloned mini-circle probes specific for the Leishmania mexicana and Leishmania braziliensis complexes were used. The results showed that L. braziliensis is the predominant group infecting human patients in the state. Sixty-eight samples were typed, 64 samples (94.1%) belonging to the L. braziliensis complex and only four (5.9%) belonging to the L. mexicana complex. Accurate identification of the Leishmania permits better orientation of the medical follow-up, since clinical manifestations may vary depending on the complex to which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Leishmaniasis; DNA hybridisation; Mato Grosso state.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700030

Effects of Storage and Exogenous Ga3 on Lychee Seed Germination
Provedor de dados: 52
Autores: Ono,Elizabeth Orika; Leonel,Sarita; Duarte Filho,Jayme; Rodrigues,João Domingos.
The effects of storage time and exogenous gibberellic acid on lychee seeds germination were studied. The seeds were removed from ripe fruits, washed, dried, stored at 8°C for 0, 15 and 30 days, and soaked during 24 hours in water and GA3 at 50, 100 and 200 mg.L-1 solutions. As the storage period increased, the germination capacity was lost, and the seeds had a short germinability after 30-day storage period. Gibberellic acid had not significant effect on enhancing both the germination percentage and rate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gibberellic acid; Gibberellins; Deterioration; Viability; Recalcitrant seed.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400015

Impact of Anthropogenic Activities on Water Quality of The Paranhana River, Southern Brazil
Provedor de dados: 52
Autores: Dalzochio,Thaís; Souza,Mateus Santos de; Simões,Leonardo Airton Ressel; Silva,Gláucia Joselaine Herbert; Rodrigues,Gabriela Zimmermann Prado; Andriguetti,Natália Bordin; Silva,Luciano Basso da; Gehlen,Günther.
Abstract: The Paranhana River, located in Southern Brazil, is one of the major tributaries of the Sinos River basin and receives mainly industrial and domestic effluents. In the present study, water physicochemical and microbiological analyses, condition factor, micronucleus test, gill histopathology and metal bioaccumulation in the muscle of the native fish Bryconamericus iheringii collected at two sites (S1 and S2) of the Paranhana River under different degrees of anthropogenic pressures were assessed in four sampling campaigns. Data from water quality parameters, condition factor, mucous cells proliferation in fish gills and bioaccumulation of chromium and manganese in muscle evidenced higher impacts at S2, whereas a higher genotoxic potential was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Paranhana River; Bioaccumulation; Histopathology; Micronucleus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100421

Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains
Provedor de dados: 52
Autores: Oliveira,Arlem Nascimento de; Oliveira,Luiz Antonio de; Andrade,Jerusa Souza.
Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-mid- exponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 ºC. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizobium; Bradyrhizobium; Amylase production; Enzymatic properties.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100005

Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive
Provedor de dados: 52
Autores: Soto-Cruz,Oscar; Chávez-Rivera,Rafael; Saucedo-Castañeda,Gerardo.
Continuous culture study of the ruminal bacterium Megasphaera elsdenii using lactate as carbon source is reported. Butyrate, the minor product in batch culture, was the main product in continuous culture. Under steady state conditions the biomass concentration was increased from 0.46 to 0.72 g l-1. Addition of Yea Sacc did not result an increased availability of carbon. The produced amounts of valerate and CO2 were calculated by using a combined carbon and degree of reduction balances. According to these results, the lactate catabolism of M. elsdenii was changed by the presence of yeast additive, yielding more butyrate and diminishing the production of valerate, while acetate and propionate production was not affected. Results reported in this work...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Megasphaera elsdenii; Butyrate; Yeast additive; Continuous culture.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200011

Influence of osmotic dehydration on ascorbic acid loss in pickled dry peppers (Capsicum chinense)
Provedor de dados: 52
Autores: Silva,Tissiane Mayara da; Argandoña,Eliana Janet Sanjinez; Madrona,Grasiele Scaramal; Moraes,Izabel Cristina Freitas; Haminiuk,Charles Windson Isidoro; Branco,Ivanise Guilherme.
The objective of this work was (1) to develop a dehydrated pepper with 45% humidity, determining the drying curves for pepper, with and without osmotic pre-treatment and (2) to evaluate the influence of both drying and osmotic treatment on the content ascorbic acid (vitamin C) in fresh pepper and pepper with 45% humidity. The experiments were carried out using the peppers cut in half, with and without osmotic pre-treatment, followed by drying in an oven at 70 ºC. The results showed that the osmotic pretreatment did not influence the retention of ascorbic acid during the drying of pepper. The sensory analysis regarding the color, flavor, and texture attributes revealed that there was no difference in the acceptability.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum chinense; Ascorbic acid; Osmotic dehydration; Drying.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500016

Evaluation of hela cell lineage response to β radiation from Holmium-166 embedded in ceramic seeds
Provedor de dados: 52
Autores: Valente,Eduardo Sarmento; Cuperschmid,Ethel Mizrahy; Campos,Tarcisio Passos Ribeiro de.
This work studied the effects of β radiation of Ho-166 embedded in ceramic seeds on HeLa cells. Methodology consisted in the production of ceramic seeds with holmium-165 by sol-gel route. Chemical and physical characterizations of the seeds were performed. Subsequently, nuclear characterization was performed by gamma spectrometry. Experimental and theoretical activities were defined and initial dose rate were evaluated by MIRD (Medical Internal Radiation Dose Committee) methodology. The seeds were placed in confluent culture flasks and remained for six radionuclide half-lives. Biological results were represented by a clean 6 mm diameter area around the seed where the tumour cells were killed. The initial dose rate was 15.5 Gy. h-1. The maximum absorbed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachytherapy; Ceramic seeds; Holmium; Cancer control; HeLa cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500013

Apple wine processing with different nitrogen contents
Provedor de dados: 52
Autores: Alberti,Aline; Vieira,Renato Giovanetti; Drilleau,Jean Françoise; Wosiacki,Gilvan; Nogueira,Alessandro.
The aim of this work was to evaluate the nitrogen content in different varieties of apple musts and to study the effect of different nitrogen concentrations in apple wine fermentation. The average total nitrogen content in 51 different apples juices was 155.81 mg/L, with 86.28 % of the values above 100 mg/L. The apple must with 59.0, 122.0 and 163.0 mg/L of total nitrogen content showed the maximum population of 2.05x 10(7); 4.42 x 10(7) and 8.66 x 10(7) cell/mL, respectively. Therefore, the maximum fermentation rates were dependent on the initial nitrogen level, corresponding to 1.4, 5.1 and 9.2 g/L.day, respectively. The nitrogen content in the apple musts was an important factor of growth and fermentation velocity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple juice fermentated; Fermentation rate; Cider; Yeast.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300017

Structural and ecophysiological alterations of the water hyacinth [Eichhornia crassipes (Mart.) Solms] due to anthropogenic stress in Brazilian rivers
Provedor de dados: 52
Autores: Vitória,Angela Pierre; Lage-Pinto,Frederico; Silva,Leonardo Bernardo Campaneli da; Cunha,Maura da; Oliveira,Jurandi Gonçalves de; Rezende,Carlos Eduardo; Souza,Cristina Maria Magalhães de; Azevedo,Ricardo Antunes.
In this work, the structural and ecophysiological alterations (chlorophyll a fluorescence and photosynthetic pigments), and quantification of Cr, Pb and Zn in the leaf limb, petiole and younger and older roots of water hyacinth from the lower, medium and upper Paraíba do Sul river (PSR) and Imbé river were evaluated. The plants from the medium and upper PSR (more industrialized and populated regions) exhibited lower turgid cell in the root cortex, less root hairs and leaf epidermis, chloroplasts with plastoglobules and increased stroma volume. Higher concentrations of metals were observed in the younger and older roots from the medium PSR plants. The results suggested that the plants from more anthropized regions were able to maintain the maximum quantum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorophyll a fluorescence; Photosynthetic pigments; Energy dissipation; Morphological alterations; Metal evaluate.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500025

Face Image Retrieval of Efficient Sparse Code words and Multiple Attribute in Binning Image
Provedor de dados: 52
Autores: S,Suchitra.
ABSTRACT In photography, face recognition and face retrieval play an important role in many applications such as security, criminology and image forensics. Advancements in face recognition make easier for identity matching of an individual with attributes. Latest development in computer vision technologies enables us to extract facial attributes from the input image and provide similar image results. In this paper, we propose a novel LOP and sparse codewords method to provide similar matching results with respect to input query image. To improve accuracy in image results with input image and dynamic facial attributes, Local octal pattern algorithm [LOP] and Sparse codeword applied in offline and online. The offline and online procedures in face image...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Face Retrieval; LOP; Multiple face attribute; Sparse representation; Indexing.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100604

Estimation models of variance components for farrowing interval in swine
Provedor de dados: 52
Autores: Cavalcante Neto,Aderbal; Lui,Jeffrey Frederico; Sarmento,José Lindenberg Rocha; Ribeiro,Maria Norma; Monteiro,José Mauro Costa; Fonseca,Carlos; Tonhati,Humberto.
The main objective of this study was to evaluate the importance of including maternal genetic, common litter environmental and permanent environmental effects in estimation models of variance components for the farrowing interval trait in swine. Data consisting of 1,013 farrowing intervals of Dalland (C-40) sows recorded in two herds were analyzed. Variance components were obtained by the derivative-free restricted maximum likelihood method. Eight models were tested which contained the fixed effects(contemporary group and covariables) and the direct genetic additive and residual effects, and varied regarding the inclusion of the maternal genetic, common litter environmental, and/or permanent environmental random effects. The likelihood-ratio test indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Common litter; Maternal effect; Permanent environment; Heritability; Repeatability; Swine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100009

Protective effects of Spirulina on the liver function and hyperlipidemia of rats and human
Provedor de dados: 52
Autores: El-Sheekh,Mostafa Mohamed; Hamad,Saied Mohamed; Gomaa,Mahmoud.
In the present study, the effects of Spirulina on subchronic treatments (two weeks) of hyperlipidemia and liver function of the rats and humans were investigated. The hyperlipidemia was induced in the rats using 25% of soya bean oil and 25% butter. The butter induced more hyperlipidemia than soya bean oil. Spirulina was used at the concentrations of 0, 2.5, 5.0 and 10 % of diet weight of the rats. The decrease in hyperlipidemia by Spirulina was dependent on its concentration in the diet. In case of human studies, about four g/day of Spirulina was taken via oral administration by Egyptian volunteers patients with hyperlipidemia. Spirulina decreased the levels of hyperlipidemia in these patients. The effects were dependent on the amount and number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spirulina; Hyperlipidemia; Protective effects; Liver functions.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100012

Bathymetric trends of northeastern Brazilian snappers (Pisces, Lutjanidae): implications for the reef fishery dynamic
Provedor de dados: 52
Autores: Frédou,Thierry; Ferreira,Beatrice Padovani.
The investigation of bathymetric distribution of five snappers caught along the Brazilian Northeastern coast by artisanal fleets through the analysis of the catch composition and relative abundance (CPUE) showed that, on the overall, fished mean size increased along depth and that particular species dominated the catch according to the depth strata. Mutton snapper, L. analis, yellowtail snapper, L. chrysurus, and dog snapper, L. jocu were mainly caught at intermediate depth (20-80m) whereas lane snapper, L. synagris, and silk snapper, L. vivanus, inhabit respectively shallow (&lt;20m) and deep (&gt;80 m) waters. Each fleet category exploited preferentially a particular combination of species and their size range. The fleet dynamic of the Northeast Brazil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish distribution; Technological interaction; Artisanal fishery; Lutjanids; Reefs.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600015

Production of biopolymers by Pseudomonas aeruginosa isolated from marine source
Provedor de dados: 52
Autores: Jamil,Nazia; Ahmed,Nuzhat.
Two bacterial strains, Pseudomonas aeruginosa CMG607w and CMG1421 produce commercially important biopolymers. CMG607w isolated from the sediments of Lyari outfall to Arabian Sea synthesize the mcl-polyhydroxyalkanoates from various carbon sources. The production of PHAs was directly proportional to the incubation periods. Other strain CMG1421, a dry soil isolate, produced high viscous water absorbing extracellular acidic polysaccharide when it was grown aerobically in the minimal medium containing glucose or fructose or sucrose as sole source of carbon. The biopolymer had the ability to absorb water 400 times more than its dry weight. This property was superior to that of currently used non-degradable synthetic water absorbents. It acted as salt filter and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopolymers; Exopolysaccharides; Hydroabsorbent; Mcl-polyhydroxyalkanoates; Pseudomonas aeruginosa; Sodium gluconate.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300004

Genotoxicity Evaluation of Dipotassium -Trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), in Human Lymphocyte Cultures and Mice Reticulocytes
Provedor de dados: 52
Autores: Haveric,Sanin; Hadzic,Maida; Haveric,Anja; Mijanovic,Mirjana; Hadziselimovic,Rifat; Galic,Borivoj.
ABSTRACT Genotoxic effects of inorganic molecule dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), a promising new therapeutic for the epidermal changes treatment, have been evaluated. In vitro analysis included evaluation of genotoxic and cytotoxic potential of K2(B3O3F4OH) in concentrations of 0.01, 0.02, 0.05 and 0.06 mg/mL applying cytokinesis-block micronucleus cytome assay in human lymphocyte culture. With the increase of concentration the frequency of micronuclei elevated but the differences were not significant. Also, there were no significant differences among the frequencies of nuclear buds and nucleoplasmic bridges between controls and treated cultures. Nuclear division index and nuclear division cytotoxycity index values did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Micronuclei; Halogenated boroxine; Human lymphocyte culture; BALB/c mice.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100348

Environmental factors affecting sporulation of Fuligo septica (Myxomycetes) on sugar cane bagasse
Provedor de dados: 52
Autores: Chiappeta,Alda de Andrade; Sena,Kêsia Xisto Fonseca R. de; Cavalcanti,Laise de Holanda.
The influence of environmental factors on sporulation of Fuligo septica (L.) Wigg. and the abundance of this species on sugar cane bagasse (Saccharum officinarum L.), stored outdoors was studied.In Northeastern Brazil, between January/1997 and January/1998, a total of 29 specimens were collected through monthly collections of aethalia. The relationships between the abundance of aethalia and rainfall, temperature, relative humidity of the air and insolation were studied. Results indicated that on the substrate analyzed, F. septica was an abundant species. Sporulation occurred in all seasons of the year, with a well-defined peak at the end of winter and beginning of spring (August/September),which was strongly influenced by rainfall.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fuligo septica; Myxomycetes; Phenology; Environmental factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100002

Nematicidal activity of leaf extracts from Lantana camara L. against Meloidogyne incognita (kofoid and white) chitwood and its use to manage roots infection of Solanum melongena L.
Provedor de dados: 52
Autores: Ahmad,Faheem; Rather,Mohmad Azhar; Siddiqui,Mansoor Ahmad.
Various concentrations of aqueous leaf extract of Lantana camara were assessed in vitro conditions against second stage juveniles (J2) of Meloidogyne incognita. The standard concentration 'S' of leaf extract was found to be highly nematostatic, where nematodes were completely paralyzed after 12 h and after 48 h of exposure, 96% of juveniles were killed at same concentration. However, the mortality of juveniles was 75% in S/2 dilution at 48 h. The degree of effectiveness and dilutions of extract were directly proportional. The percentage of paralyzed juveniles was decreased, when J2 transferred in distilled water after 48 h incubation in standard aqueous leaf extract. Addition of freeze-dried aqueous extract to sterile sandy substrate at S/2 dilution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eggplant; Root-knot nematode; Nematostatic activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300006

Investigation of Structural, Morphological, Magnetic Properties and Biomedical applications of Cu2+ Substituted Uncoated Cobalt Ferrite Nanoparticles
Provedor de dados: 52
Autores: Margabandhu,M.; Sendhilnathan,S.; Senthilkumar,S.; Gajalakshmi,D.
ABSTRACT In the present work, Cu2+ substituted cobalt ferrite (Co1-xCuxFe2O4, x = 0, 0.3, 0.5, 0.7 and 1) magnetic nanopowders were synthesized via chemical co-precipitation method. The prepared powders were investigated by various characterization methods such as X-ray diffraction analysis (XRD), scanning electron microscope analysis (SEM), vibrating sample magnetometer analysis (VSM) and fourier transform infrared spectroscopy analysis (FTIR). The XRD analysis reveals that the synthesized nanopowders possess single phase centred cubic spinel structure. The average crystallite size of the particles ranging from 27-49 nm was calculated by using Debye-scherrer formula. Magnetic properties of the synthesized magnetic nanoparticles are studied by using VSM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Co-precipitation; Cubic spinel; Morphology; Anti-bacterial activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300401

Reproductive biology of the dusky grouper Epinephelus marginatus (Lowe, 1834)
Provedor de dados: 52
Autores: Andrade,Áthila Bertoncini; Machado,Leonardo Francisco; Hostim-Silva,Maurício; Barreiros,João Pedro.
Investigations were carried out about the reproductive biology of the dusky grouper, Epinephelus marginatus (Lowe, 1834) a serranid protogynous hermaphrodite fish inhabitant of rocky bottoms from shallow waters up to 200 m depth. Samples were caught in the islands that form Arvoredo Biological Marine Reserve (SC - Brazil) and the coastal islands near Florianópolis region from March 1998 to May 2000. Data from samples of juvenile groupers on intertidal pools were used to link their occurrence in the pools to the post-spawning season. To describe the histological changes on gonads along the reproductive cycle and to establish maturation scales, 109 groupers were histologically tested for sex determination. First maturation size was determined for females...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproduction; Dusky Grouper; Epinephelus marginatus; Santa Catarina; Brazil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300009

Biodegradation of crude petroleum oil and environmental pollutants by Candida tropicalis strain
Provedor de dados: 52
Autores: Farag,Soha; Soliman,Nadia A.
A local yeast isolate named A was isolated from polluted area of Abou-Qir gulf (Alexandria, Egypt), identified according to a partial sequence of 18sRNA as Candida tropicalis. The isolate showed a high potency in petroleum oil biodegradation as well some hydrocarbons. Morphological changes in cell diameter of this yeast were recognized upon growing the target cell in sea water medium supplemented with petroleum oil as sole carbon source in comparison to the growth in enriched medium. Statistically-based experimental design was applied to evaluate the significance of factors on petroleum oil biodegradation by this yeast isolate. Eleven culture conditions were examined by implementing Plackett-Burman factorial design where aeration, NH4Cl and K2HPO4 had the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Petroleum oil; Candida tropicalis; 18Sr RNA; Biodegradation; Plackett-Burman.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400023

In vitro cytotoxic, genotoxic and antioxidant/oxidant effects of guaiazulene on human lymphocytes
Provedor de dados: 52
Autores: Toğar,Başak; Çelik,Kübra; Türkez,Hasan.
The aim of this study was to evaluate for the cytotoxicity, genotoxicity and antioxidant/oxidant activity of GYZ on human peripheral blood lymphocytes (PBLs). Guaiazulene (GYZ) was added into culture tubes at various concentrations (0-400 µg/mL-1). Cytotoxicity against the human lymphocytes cultures was examined by lactate dehydrogenase (LDH) release assay. The proliferative response was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Antioxidant/oxidant activity was evaluated by measuring the total oxidant status (TOS) and total antioxidant capacity (TAC) levels. Micronucleus (MN) and chromosomal aberration (CA) tests were used in genotoxicity studies. The results showed that GYZ caused cytotoxicity in the PBLs at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guaiazulene; Cytotoxicity; Genotoxicity; Antioxidant activity; Oxidant status.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100061

Uptake of cadmium by Pseudokirchneriella supcapitata
Provedor de dados: 52
Autores: Casiraghi,Magela Paula; Luporini,Samuel; Silva,Eduardo Mendes da.
In this work the microalgae Pseudokirchneriella supcapitata was used for the removal of the cadmium in liquids. The accumulations of metal ions by the alga occur in two stages: a very fast absorption (passive adsorption) proceeded by a slower absorption (activate absorption). A mathematical model based on the surface absorption and on the transport into the interior of the cellular membrane was developed. The simulation model kinetic parameters were experimentally obtained. Through the results observed, the mathematical model was shown to be suitable when compared to the experimental results, confirming the validation of the mathematical model.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cadmium; Microalgae; Heavy metals; Mathematical model.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800019

Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge
Provedor de dados: 52
Autores: Kim,Doo Hwan; Sivanessan,Iyyakkannu.
Effects of N6-benzyladenine (BA) or thidiazuron (TDZ) on adventitious shoot regeneration and axillary shoot multiplication of Sedum sarmentosum was investigated. Leaf and shoot tip explants obtained from in vitro-grown shoots of S. sarmentosum were cultured on Murashige and Skoog (MS) medium supplemented with 0, 2.0, 4.0 or 8.0 µM BA or TDZ. Of the two cytokinins studied, BA was found to be more responsive as compared to TDZ with respect to number of shoots produced per explant. High frequency of shoot regeneration (92.2%) with a mean of 12.3 shoots was produced when the leaf explants were cultured on MS medium supplemented with 8.0 µM BA. The highest number of shoots (25.4) was obtained when shoot tip explants were cultured on MS medium devoid of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crassulaceae; Cytokinin; Direct shoot regeneration; Shoot multiplication; Stringy stonecrop.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100418

Drift of ichthyoplankton in two channels of the Paraná River, between Paraná and Mato Grosso do Sul states, Brazil
Provedor de dados: 52
Autores: Bialetzki,Andréa; Sanches,Paulo Vanderlei; Cavicchioli,Maristela; Baumgartner,Gilmar; Ribeiro,Ricardo Pereira; Nakatani,Keshiyu.
Analysis of drift of ichthyoplankton in two channels in the River Paraná, Brazil, were made. Sampling was undertaken from October 1995 to April 1996 during nychthemeral cycles. Water samples were collected to determine several environmental variables. Eggs densities largely differed to layers, times of day and months. In both the channels, highest occurrence of eggs was detected between January and March; greater concentrations at the bottom, during night. With regard to larvae, densities were significantly different in channels, times of days and months. Maximum occurrences were seen in the right channel, in both layers, with largest capture between January and March. Surface of the both channels presented highest density of larvae during night. Water...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drift; Ichthyoplankton; Paraná River.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100008

Evaluation of oat extracts on the efficiency of lime in soil
Provedor de dados: 52
Autores: Cassiolato,Marcelo E.; Meda,Anderson R.; Pavan,Marcos A.; Miyazawa,Mário; Oliveira,José C. de.
The low mobility of the soluble components of surface-applied lime, limits their ability to reduce subsoil acidity in variable charge soils. Laboratory experiments were conducted with brazilian Oxisol to evaluate the effect of oat extracts on the mobility of surface applied calcium in form of CaCO3. The following oat cultivars were evaluated: Argentina 5VL3, UPF 90H400-2, IAPAR 61, IA96101-b, SI 83400, EMBRAPA 29, LD 9102, FAPA 1, ER 93247, ER 90148, ER 89144, ER 93152, Alpha 94124, Alpha 94206 and Preta comum. Oat extracts and lime were applied at rates of 10 and 5 Mg ha-1, respectively. Effect of lime without plant extracts on soil acidity was limited in the upper 10 cm of the profile. Lime in the presence of oat extracts increased pH and Ca ex and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subsoil acidity; Ca-leaching; Plant residue.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500014

Genes Regulating Maternal Recognition of Pregnancy in Domestic Animals: an Update
Provedor de dados: 52
Autores: Mor,Avantika; Mondal,Sukanta; Reddy,Ippala Janardana; Soumya,N.P.
ABSTRACT Early embryonic mortality is one of the main sources of reproductive wastages and major constraints for full exploitation of the production potential of livestock. The survivality of embryo during early embryonic life is mostly dependent on the efficiency with which the maternal recognition of pregnancy (MRP) is established. Maternal recognition of pregnancy involves molecular dialogue between the trophoblast of conceptus and uterine endometrium. Embryonic development to the blastocyst stage and uterine differentiation to the receptive environment are crucial for successful establishment of the embryo-uterine cross-talk that leads to the initiation and progression of successful implantation. Unravelling the complex intricate molecular and cellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryonic mortality; Maternal recognition of pregnancy; Embryo-uterine cross-talk.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600854

Hepatic Cytochrome P450 as Biomarkers of Cypermethrin Toxicity in Freshwater Teleost, Channa punctatus (Bloch)
Provedor de dados: 52
Autores: Bhutia,Dawa; Rai,Benoy Kishore; Pal,Joydeb.
In this study, Channa punctatus was treated with sub-lethal concentration of cypermethrin (6.6 µg/L) for 5, 10 and 15 days and its effect on total CYP 450 and the activity of hepatic CYP450 isoforms measured. Total CYP450 content and CYP1A mediated EROD activity was significantly induced (p&lt;0.05) in all three treated groups compared to control whereas only 15 days treated group showed significant induction in CYP2B mediated N,Ndimethylaniline demethylase activity. CYP2E1 mediated aniline hydroxylase activity showed only a marginal increase while there was inhibition of CYP3A4 mediated erythromycin demethylase activity. Liver somatic index (LSI) also showed a marginal increase in all the treated groups. Results showed differential induction of CYP1A,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cypermethrin; Cytochrome P450; Fish; Channa punctatus; Biomarker.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100131

Quality Control of Trichogramma atopovirilia and Trichogramma pretiosum (Hym.: Trichogrammatidae) adults reared under laboratory conditions
Provedor de dados: 52
Autores: Soares,Marcus Alvarenga; Leite,Germano Leão Demolin; Zanuncio,José Cola; Sá,Veríssimo Gibran Mendes de; Ferreira,Cleidson Soares; Rocha,Silma Leite; Pires,Evaldo Martins; Serrão,José Eduardo.
The objective of this work was to assess the flight capacity, parasitism and emergence of Trichogramma atopoviriliaand two strains of T. pretiosum (Hymenoptera: Trichogrammatidae) (L1 and L2). The flight capacity of these species was assessed in test units consisting of a plastic PVC cylinder with a rigid transparent plastic circle on the upper part and an extruded polystyrene disk closing its bottom. A tube was placed in each test unit containing a card with 300 Anagasta kuehniella (Lepidoptera: Pyralidae) eggs parasitised by Trichogramma. These cards were assessed to determine the parasitism rate and adult emergence of these natural enemies. T. atopovirilia and T. pretiosum L1 presented adequate flight capacity and parasitism, in addition to high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Flight; Parasitism; Quality control; Emergence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200018

Populational Diversity on Leaf Morphology of Maté (Ilex paraguariensis A. St.-Hil., Aquifoliaceae)
Provedor de dados: 52
Autores: Coelho,Geraldo Ceni; Mariath,Jorge Ernesto de Araujo; Schenkel,Eloir Paulo.
Maté (Ilex paraguariensis A. St.-Hil.) is a native plant from southern Brazil and a raw material for beverages. To research on population variability of the species, we compared leaf morphology of three populations from Mato Grosso do Sul (MS), Paraná (PR) and Santa Catarina (SC) States. Lamina measurements included area (A), perimeter (P), length (l), maximum width (w), distance between maximum width and lamina base (dw), base angle (BÂ), biomass (m), and the indexes: relative distance of maximum width (dw/l), ratio length/width (l/w), relative perimeter (RP= P / (l + w), rectangular area index (RAI = A / (l x w)) and leaf mass/area (m/A). We verified differences among populations on A (MS&lt;PR=SC), l (MS&lt;PR=SC), w (MS&lt;PR=SC), RAI (MS=PR&gt;SC) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Aquifoliaceae; Plant morphology; Leaf morphology; Population variability; Rectangular area index.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100008

Partial purification and characterization of xylanase produced by Penicillium expansum
Provedor de dados: 52
Autores: Querido,André Luiz de Souza; Coelho,Jorge Luiz Cavalcante; Araújo,Elza Fernandes de; Chaves-Alves,Virgínia Maria.
An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. The enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. The protein eluation profile showed only one form of xylanase that was partially characterized. The activity of purified xylanase was optimal at pH 5.5 and 40 ºC. The enzyme was stable at pH between 5.5 and 6.5 and temperatures between 20-40 ºC. The enzyme showed a Km of 3.03 mM and Vmax of 0.027 mumol min-1 mug -1 of protein. The enzymatic activity was increased 31 % by Mg2+ and 28 % by Al3+.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partial purification; Xylan/xylanases; Penicillium expansum.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400016

Certain Aspects of Univalent Function with Negative Coefficients Defined by Bessel Function
Provedor de dados: 52
Autores: Ramachandran,Chellakutti; Dhanalakshmi,Kaliyaperumal; Vanitha,Lakshminarayanan.
ABSTRACT Key words: In recent years, applications of Bessel functions have been effectively used in the modelling of chemical engineering processes and theory of univalent functions.In this paper, we study a new class of analytic and univalent functions with negative coefficients in the open unit disk defined by Modified Hadamard product with Bessel function. We obtain coefficient bounds and exterior points for this new class.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Analytic function; Univalent function; Extreme point; Bessel function; Subordination; Hadamard Product.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300603

Selection of Xilose-Fermenting Yeast Strains
Provedor de dados: 52
Autores: Silva,Rosimeire Oenning da; Cereda,Marney Pascoli; Gomes,Eleni; Martins,Gisele Marta; Pagnocca,Fernando Carlos; Silva,Roberto da.
ABSTRACT In Brazil, ethanol is obtained by fermentat of sugar cane juice using Saccharomyces cerevisiae. The cane juice extraction generates the bagasse that has been used for obtaining generation biofuel. However, the sugarcane bagasse has 30% pentose that cannot be fermented to ethanol by S. cerevisiae. Thus the aim of this study was to isolate a yeast able to ferment xylose to ethanol. Samples of cane juice and flowers were used for the isolation of 165 strains that were then screened for ethanol production using plate testing. Among them, the ethanol positive strains Wickerhamomyces anomalus, Schizosaccharomyces pombe and Starmerella meliponinorum were selected for a xylose fermentation assay, using a semi-synthetic and bagasse hydrolysate as must. S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylose; Ethanol; Yeast; Sugarcane bagasse.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100419

Biofumigation Studies by Using Raphanus sativus and Eruca sativa as a Winter Cycle Crops to Control Root-knot Nematodes
Provedor de dados: 52
Autores: Aydınlı,Gökhan; Mennan,Sevilhan.
ABSTRACT An experiment was conducted to search the efficacy of radish (Raphanus sativus) and arugula (Eruca sativa) for the control of Meloidogyne arenaria in the commercial tomato growing greenhouse. R. sativus and E. sativa were used as winter cycle crops and lettuce as a susceptible crop and 4 months after sowing, host level of the treatment plants was evaluated. All parts of R. sativus and E. sativa were incorporated except lettuce was covered with transparent polyethylene film for 4 weeks. R. sativus and E. sativa had not any root galls, and these plants caused reducing number of juveniles in the soil, in contrast to control and lettuce plots before growing tomato. Gall index and egg masses were significantly decreased on tomatoes in plots of applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meloidogyne arenaria; Raphanus sativus; Eruca sativa; Biofumigation; Crop yield.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100205

Sedative and anxiolytic effects of methanolic extract from the leaves of Passiflora actinia
Provedor de dados: 52
Autores: Santos,Kely Cristina dos; Kurtz,Stella Maris Tessaro Figura; Müller,Simony Davet; Biavatti,Maique Weber; Oliveira,Rúbia Maria Monteiro Weffort de; Santos,Cid Aimbiré de Moraes.
Leaves from several Passiflora species are largely employed in the Brazilian folk medicine as anxiolytic and sedative. In this work the anxiolytic, sedative-like properties and liquid chromatography analysis of methanolic extract of Passiflora actinia were evaluated. The methanol extract and all of its fractions presented significant sedative-like effect in elevated plus-maze and open field tests. Only the aqueous fraction of the methanol extract showed selective anxiolytic activity (30 mg/kg). Chromatographic analysis of the active fractions showed the presence of isovitexin and absence of the classical Passiflora beta-carboline alkaloids or flavonoids such as vitexin, rutin, swertisin, hesperidin and orientin. The tincture obtained from P. actinia leaves...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiolytic; Sedative; Isovitexin; Passiflora actinia; Passifloraceae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500005

Effect of tillage systems and permanent groundcover intercropped with orange trees on soil enzyme activities
Provedor de dados: 52
Autores: Balota,Elcio Liborio; Machineski,Oswaldo; Truber,Priscila Viviane; Auler,Pedro Antonio Martins.
The objective of this study was to evaluate the effect of different soil tillage systems and groundcover crops intercropped with orange trees on soil enzyme activities. The experiment was performed in an Ultisol soil in northwestern Paraná State. Two soil tillage systems were evaluated [conventional tillage (CT) across the entire area and strip tillage (ST) with a 2-m strip width] in combination with various groundcover vegetation management systems. Soil samples were collected after five years of experimental management at a depth of 0-15 cm under the tree canopy and in the inter-row space in the following treatments: (1) CT-Calopogonium mucunoides; (2) CT-Arachis pintoi; (3) CT-Bahiagrass; (4) CT-Brachiaria humidicola; and (5) ST-B. humidicola. The soil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arylsulfatase; Acid phosphatase; Tillage systems; Vegetative cover.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200001

Stability of phenolic compounds and antioxidant capacity of regular and decaffeinated coffees
Provedor de dados: 52
Autores: Vicente,Silvio José Valadão; Queiroz,Yara Severino; Gotlieb,Sabina Léa Davidson; Torres,Elizabeth Aparecida Ferraz da Silva.
This study compared the regular and decaffeinated coffees in relation to antioxidant capacity, levels of some antioxidant molecules and stability of these parameters over a six-month period under different storage conditions. The regular coffee samples analyzed right after the industrial production showed higher antioxidant capacity (ORAC and DPPH), the same levels of phenolic compounds and higher levels of phenolic acids than decaffeinated coffee. After six months, the closed packs of both the grades kept under vacuum at 20°C did not show significant changes from the initial results; the open packs stored at 4°C showed small but statistically significant reductions and the open packs stored at 20°C showed higher and statistically significant losses (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Antioxidant capacity; Phenolic compounds; Caffeine; ORAC; DPPH.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100016

Essential oil yield and composition of Lamiaceae species growing in southern Brazil
Provedor de dados: 52
Autores: Agostini,Fabiana; Santos,Ana Cristina Atti dos; Rossato,Marcelo; Pansera,Márcia Regina; Santos,Paula Luciana dos; Serafini,Luciana Atti; Molon,Rosângela; Moyna,Patrick.
The Lamiaceae family has been described to be rich in essential oil. The aim of the present work was to identify the native species of Rio Grande do Sul with potential use as the aromatic plants. Seven species of the family were collected in the different localities of the state. The essential oils were obtained by 1h hydrodistillation in a Clevenger apparatus. The constituents were identified by comparing their GC Kovats retention indices (RI), determined with reference to a homologous series of n-alkanes and by comparing their mass spectral fragmentation patterns with literature data. Cunila incisa and Mentha aquatica resulted in the higher esssential oil yields (1.94% v/w and 0.93% v/w, respectively. The composition of M. aquatica was 80% methone, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lamiaceae species; Essential oil composition; Southern Brazil; Hydrodistillation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200026

Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti
Provedor de dados: 52
Autores: Machado,Marcelo Rubens; Fanta,Edith.
Gills are vital structures for fish, since they are the main site for gaseous exchange as well as partially responsible for osmorregulation, acid-basic balance, excretion of nitrogenous compounds and taste. Chemicals in the water may alter the morphology of branchial cells of fish that are, therefore, a useful model for environmental impact and ecotoxicology studies. In order to investigate the effects of an organophosphorous compound, methyl parathion, on the gills of the fish, samples of Metynnis roosevelti were exposed to lethal (7ppm) and sublethal (1ppm) doses of Mentox 600 CE. Through light and scanning electron microscopy, shrinking of the branchial epithelium, followed by detachment and hyperplasia were observed. Externally, the branchial filaments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gill; Fish; Methyl parathion; Toxicity; Morphology.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300008

Semi-hypogeal germination in Pachyrhizus ahipa (Wedd.) parodi (Fabaceae: Phaseoleae): seedling and sapling morphology
Provedor de dados: 52
Autores: Milanez,Camilla R. Dias; Oliveira,Denise M. Trombert; Moraes-Dallaqua,Marina A.
The aim of this work was to describe the morphology of seedlings and saplings of Pachyrhizus ahipa, a cultivated species of Phaseoleae (Fabaceae), analysing the seedling type and characterizing the structure of cotyledons, eophylls and metaphylls. It was observed that the seedling was semi-hypogeal, a germination type not yet recorded for the Phaseoleae tribe. It formed two opposite and unifoliated eophylls with an evident pair of stipels. Metaphylls were trifoliolate and had alternate phyllotaxis. Both eophylls and metaphylls were pinnate, camptodromous, and brochidodromous.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphology; Phaseoleae; Sapling; Seedling; Eophyll; Metaphyll.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200015

Role of intermediary cells in Peltodon radicans (Lamiaceae) in the transfer of calcium and formation of calcium oxalate crystals
Provedor de dados: 52
Autores: Paiva,Elder Antônio Sousa; Machado,Silvia Rodrigues.
With the objective of studying the connection between calcium oxalate crystals formation and the phloem, fragments of leaves of Peltodon radicans Pohl (Lamiaceae) were fixed and processed, for light and electron-transmission microscopes. It was observed that the crystals occurred in the cells of the bundle sheath, juxtaposed in relation to the phloem. Intermediary cells established a connection between the sieve element and crystal-bearing sheath cells. Calcium was present abundantly in the cytoplasm of sheath cells as calcium oxalate crystals. The presence of calcium was also detected in the intermediary cells, but in the sieve elements it was not detected. There was, therefore, an increasing concentration gradient of calcium in the sieve elements from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium oxalate; Calcium metabolism; Companion cells; Crystals; Peltodon radicans; Phloem.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100019

Physicochemical Characterization, Antioxidant Potential and Sensory Quality of Wine from Wild Edible Fruits of Flacourtia montana J. Graham
Provedor de dados: 52
Autores: Mundaragi,Abhishek; Thangadurai,Devarajan; Appaiah,Konerira Aiyappa Anu; Dandin,Chethan Jambanna; Sangeetha,Jeyabalan.
Abstract The objective of the present study was to produce wine from wild edible fruits of Flacourtia montana J. Graham. The various physicochemical attributes including total phenolic content and total flavonoid content were analyzed. Further, the prepared wine was evaluated for the antioxidant potential using four different assays, viz., 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay and total antioxidant activity. Finally, the wine was subjected for the sensory evaluation. Experimental results revealed that wine had an alcohol content of 7.20%, total phenolic content of 0.776±0.032 mg GAE/ml and total flavonoids of 0.121±0.012 mg QE/ml. High performance liquid chromatography...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flacourtia montana; Underutilized fruit; Fruit wine; Polyphenols; Antioxidant activity; Sensory evaluation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100507

Spray-drying of passion fruit juice using lactose-maltodextrin blends as the support material
Provedor de dados: 52
Autores: Angel,Ruiz Cabrera Miguel; Espinosa-Muñoz,Lucía Carolina; Aviles-Aviles,Carlos; González-García,Raúl; Moscosa-Santillán,Mario; Grajales-Lagunes,Alicia; Abud-Archila,Miguel.
The objective of this study was to assess the effectiveness of the blends with different levels of lactose-maltodextrin (8:5, 10:5, and 12:5 % w/v) during the spray-drying of the passion fruit juice. The drying was carried out in a laboratory spray dryer (Pulvis GB 22 model) at two inlet air temperatures (180 and 190 ºC), and two air pressures (0.10 and 0.20 MPa). The moisture content, hygroscopicity and vitamin C retention were evaluated in the powder obtained. Response surface plots (p&lt;0.05) showed that the lowest values of the moisture content and hygroscopicity were reached in the temperature range of 188-190 ºC and at 12:5 % (w/v) concentration of lactose-maltodextrin; the best vitamin C retention level occurred at 180 ºC and 0.2 MPa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passion fruit juice; Spray-drying; Vitamin C retention; Hygroscopicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400026

Influence of Drying Conditions on the Final Quality of Cherry and Grape Tomatoes
Provedor de dados: 52
Autores: Altino,Heitor Otacílio Nogueira; Locatelli,Karyna Maria de Mello; Cunha,Renata Nepomuceno da.
ABSTRACT The convective drying process of cherry and grape tomatoes for dried tomato production was studied taking into account operational and sensorial aspects. The tomatoes were physicochemical characterized and dried at three air temperatures in a drying chamber. Thus, it was possible to the determinate the physicochemical characteristics, drying kinetics, thickness shrinkage, effective moisture diffusivity and activation energy. The effects of tomato type (cherry and grape), air temperature (60°C and 80°C), and final moisture (25% and 35% w.b.) were sensory evaluated utilizing a factorial experiment. The drying kinetics demonstrated that the drying processes occurred preferably in the falling rate-drying periods. The grape tomato showed a faster...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cherry tomatoes; Grape tomatoes; Drying; Sensory; Kinects.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100500

Cytotaxonomy, heterochromatic polymorphism and natural triploidy of a species of Astyanax (Pisces, Characidae) endemic to the Iguaçu river basin
Provedor de dados: 52
Autores: Kantek,Daniel Luis Zanella; Noleto,Rafael Bueno; Fenocchio,Alberto Sérgio; Cestari,Marta Margarete.
Cytogenetic analysis with Astyanax sp. D revealed a karyotype of 2n=50 with 2M+26SM+6ST+16A, besides a triploid specimen showing 2n=75 chromosomes (3M+39SM+9ST+24A). C-banding strongly stained the terminal regions of several SM-ST-A chromossomes. Two pairs of acrocentric chromosomes presented interstitial heterochromatin, this state being polymorphic and occuring due to possible paracentric inversions. The results obtained with the AluI restriction enzyme and A3 chromomycin were similar to the C-banding. Relationships were proposed between Astyanax sp. D and A. scabripinnis, as well as considerations for a possible origin of the triploid specimen (2n=3x=75). When comparing the present results with cytogenetic features of other endemic Astyanax species in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Triploidy; Paracentric inversion.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100008

Effect of Heavy Metals on Liver, Kidney, Gills and Muscles of Cyprinus carpio and Wallago attu inhabited in the Indus
Provedor de dados: 52
Autores: Mahboob,Shahid; Kausar,Shazia; Jabeen,Farhat; Sultana,S.; Sultana,T.; Al-Ghanim,K.A.; Hussain,Bilal; Al-Misned,Fahad; Ahmed,Z.
ABSTRACT: The present study was aimed to evaluate the effect of heavy metals on an important tissue of two fish species Cyprinus carpio and Wallago attu, sampled from the Indus river, Mianwali District, Pakistan. The concentration of selected heavy metals Fe, Cr, Cu, and in gills, muscles, kidney and liver was compared with an International standard of food fish. The overall metal concentrations among different weight categories in C. carpio were in the order of Fe &gt; Cu &gt; Cr &gt;. In W. attu the overall accumulation of these metals were, in order of Fe &gt; Cu &gt; Cr &gt; Pb The order of accumulation of metals in gills and muscle of C. carpio was Fe &gt; Cr &gt; Pb &gt; Cu; kidney and muscles of W. attu was Fe &gt; Cr &gt; Cu &gt; Pb; liver Fe &gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: River; Heavy metals; Fish; Weight; Pollution.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100334

In Vitro Organogenesis from Root Explants of Passiflora miniata Mast., an Amazonian Species with Ornamental Potential
Provedor de dados: 52
Autores: Carvalho,Paula Pinheiro de; Antoniazzi,Camila Aparecida; Faria,Rodrigo Brito de; Carvalho,Ilio Fealho de; Rocha,Diego Ismael; Silva,Maurecilne Lemes da.
ABSTRACT The present study reports a shoot organogenesis-based system for in vitro regeneration of Passiflora miniata, an Amazonia passion fruit species. Root segments were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations (range 2-9 µM) of 6-benzyladenine (BA); thidiazuron (TDZ) or kinetin (KIN). Plant growth regulators were not added to the control treatment. Root explants have showed a high regenerative potential. After 30 days of in vitro culture, the root explants showed several shoots formed direct and indirectly. TDZ provided the best response in the differentiation adventitious shoots, mainly in the presence of 6.8 µM. The cytokinins BA and KIN responded producing a reduced number of shoots. After 120 days,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphogenesis; Ornamental passion fruit; Plant regeneration; Root explant; Shoot organogenesis; Thidiazuron.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100406

Quality of minimally processed beet roots stored in different temperatures
Provedor de dados: 52
Autores: Vitti,Maria Carolina Dario; Yamamoto,Liane Kazuko; Sasaki,Fabiana Fumi; Aguila,Juan Saavedra del; Kluge,Ricardo Alfredo; Jacomino,Angelo Pedro.
The present work was carried out with the objective to determine the effect of different storage temperature on the quality of beet root minimally processed. Respiratory activity and mass loss were higher in the storage at 15ºC. No significant differences in color, total soluble solids, betacyanin and betaxanthin were observed among treatments. Based on the results obtained, the storage at 0ºC was recommended to storage beet root minimally processed and that 10 and 15ºC drastically reduced the quality of minimally processed beet roots during storage.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta vulgaris L.; Fresh-cut; Betalains; Respiratory rate.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500001

Genetic, morphological and spatial characterization of two populations of Mabea fistulifera Mart. (Euphorbiaceae), in different successional stages
Provedor de dados: 52
Autores: Goulart,Maíra Figueiredo; Ribeiro,Sérvio Pontes; Lovato,Maria Bernadete.
Two populations of Mabea fistulifera (Euphorbiaceae) located at Rio Doce State Park (Minas Gerais, Brazil) in places characterized as in different successional stages, were investigated through genetic and ecological traits. Twenty randomly chosen individuals from each population had its genetic data assessed by 43 RAPD markers and were also evaluated through five morphologic traits and its spatial distribution. Both Shannon's index and AMOVA reveled that most of the genetic variation was found between individuals within populations and about 9% of variation was located between populations. Moreover, populations differed genetically, but presented similar values of genetic diversity. Morphologic data showed differences between populations. Significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mabea fistulifera; Genetic diversity; RAPD; Tree architecture; Spatial distribution; Successional gradient.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200015

Anti-Quorum Sensing Potential of Antioxidant Quercetin and Resveratrol
Provedor de dados: 52
Autores: Erdönmez,Demet; Rad,Abbas Yousefi; Aksöz,Nilüfer.
ABSTRACT Quorum sensing system plays an active role in the regulation of pathogenicity of many microorganisms. Inhibition of pathogenicity or virulence factors will increase the success of treatment by preventing the development of antibiotic resistance. In this study, anti-quorum sensing activities of quercetin and resveratrol compounds, which have antioxidant property without damaging to host, have been determined via using biosensor bacteria: Chromobacterium violaceum ATCC 12472 and Chromobacterium violaceum CV026. As quorum sensing inhibitors, quercetin and resveratrol's cutting off the bacterial communication will prevent the treatment failures caused by the development of bacterial resistance. The development of layered drugs with antioxidant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-quorum sensing; Resveratrol; Quercetin; Antioxidant.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100403

Radiation exposure in nuclear medicine: real-time measurement
Provedor de dados: 52
Autores: Sylvain,Iara; Bok,Bernard.
French regulations have introduced the use of electronic dosimeters for personal monitoring of workers. In order to evaluate the exposure from diagnostic procedures to nuclear medicine staff, individual whole-body doses were measured daily with electronic (digital) personal dosimeters during 20 consecutive weeks and correlated with the work load of each day. Personal doses remained always below 20 µSv/d under normal working conditions. Radiation exposure levels were highest to tech staff, nurses and stretcher-bearers. The extrapolated annual cumulative doses for all staff remained less than 10 % of the maximum legal limit for exposed workers (2 mSv/yr). Electronic dosimeters are not technically justified for routine survey of staff. The high sensitivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dosimetry; Exposure; Electronic dosimeters; Personal monitoring; Equivalent dose.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500015

Epidemiological estimates of Respiratory diseases in the hospital population, Faisalabad, Pakistan.
Provedor de dados: 52
Autores: Sultana,Tayyaba; Afzal,Aqsa; Sultana,Salma; Al-Ghanim,Khalid; Shahid,Tehniat; Jabeen,Zahra; Turab,Nusrat; Ahmed,Z.; Mahboob,Shahid.
ABSTRACT  A cross sectional study was conducted in two types of respiratory patients in hospital population. It was found that tuberculosis (T.B) was the most common type (29.66%) followed by the asthma (28.08%) and chronic obstructive pulmonary disease (COPD) (11.31%). Average age of diagnosis was 42.15 ± 0.65 years and average age at present 47.99 ± 0.70 years. Age group 51-60 years was more prone to this disease (21.13%). Most of the patients were married (80.06%). The highest representation of patients with respiratory diseases was observed in 1st birth order (30.36%) followed by 2nd (26.49%) and 3rd (18.45%), while the lowest was in 10th birth order (0.40%). Tuberculosis, asthma and COPD are the most prevalent types of respiratory diseases....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Respiratory; Age; Sex; Urban; Rural; Income.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100418

Effects of plant lectins on in vitro fibroblast proliferation
Provedor de dados: 52
Autores: Sell,Ana Maria; Costa,Celso Paulino da.
Lectins are carbohydrate-binding proteins that have been isolated from various sources and presented a wide spectrum of biological activities. The effects of four lectins, namely, Phaseolus vulgaris phytohemagglutinin, PHA, wheat germ agglutinin, WGA, Artocarpus integrifolia seed lectins, jacalin and artocarpin, on in vitro fibroblasts proliferation were investigated. The lectins did not influence the initial cell adhesion to the plate. PHA and WGA at 10-20 µg/mL concentrations significantly decreased fibroblasts proliferation. At these concentrations, they caused morphological alterations on cells and over 80 µg/mL, promoted cell death. Neither jacalin nor artocarpin significantly affected cell proliferation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lectins; Fibroblasts; Proliferation; MTT.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300006

Hydrologic pulses and remaining natural vegetation in Jaú and Jacaré-Pepira watersheds
Provedor de dados: 52
Autores: Rezende,Jozrael Henriques; Pires,José Salatiel Rodrigues; Mendiondo,Eduardo Mario.
The aim of this work was to study the influence of natural vegetation in two subwatersheds of the Tietê-Jacaré Water Resources Management Unit in São Paulo State on the pulse of their rivers. In Jacaré-Pepira Subwatershed, having higher remaining cover index, pulses were more predictable and had lower amplitude in the study period, indicating less perturbation of the water body and higher resilience of the aquatic ecosystem. Jacaré-Pepira River specific mean discharge was higher than the Q5% calculated through the Hydrologic Regionalization Method for São Paulo State, whereas Jaú River mean discharge was lower than Q5%. The minimum discharge recorded for Jacaré-Pepira River was higher than Q7.10 and equal to Q95%, whereas for Jaú River this value was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Discharge; Watershed; Remaining vegetation cover.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500017

Reproductive Strategies of Plagioscion squamosissimus Heckel, 1840 (Osteichthyes Sciaenidae) in the Itaipu Reservoir, Brazil
Provedor de dados: 52
Autores: Carnelós,Regina Cintia; Benedito-Cecilio,Evanilde.
Plagioscion squamosissimus is a dominant species in the Itaipu reservoir and has a significant role in the regional economy. The aim of the present research was the investigation of the curvina's reproductive strategies during the first years of the Itaipu reservoir (November 1983 to October 1989). Collections were undertaken from eleven sampling sites in the reservoir area, left bank tributaries, Paraná River and its tributaries. Species begin the reproduction in the area of influence of the Itaipu reservoir only in 1986. Size of early maturation, local and period of spawning showed gradual variations, with a broadening trend of this activity in the area, and thus guaranteeing success of the species. Mean size of early sexual maturation for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproduction; Itaipu reservoir; Plagioscion squamosissimus; Impact.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300010

Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok
Provedor de dados: 52
Autores: Hirose,Edson; Neves,Pedro M. O. J.; Zequi,João A. C.; Martins,Luís H.; Peralta,Cristiane H.; Moino Jr.,Alcides.
The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion &lt;FONT FACE=Symbol&gt;Ô&lt;/FONT&gt; and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion&lt;FONT FACE=Symbol&gt;Ô&lt;/FONT&gt; caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic Agriculture; Entomopathogenic Fungi; Toxic Effect.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400013

Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation
Provedor de dados: 52
Autores: Costa,Jorge Alberto Vieira; Colla,Eliane; Magagnin,Glênio; Santos,Lucielen Oliveria dos; Vendruscolo,Mauricio; Bertolin,Telma Elita.
Amyloglucosidase (AMG) and exo-polygalacturonase (exo-PG) were simultaneously produced by two different strains of Aspergillus niger in solid-state fermentation (SSF) using defatted rice-bran as substrate. The effect of Aspergillus niger strain (t0005/007-2 and/or CCT 3312), inoculum type (spore suspension or fermented bran) and addition of inducers (pectin and/or starch) to the culture media was studied using a 3² x 2¹ factorial experimental design. The production of AMG and exo-PG was significantly affected by fungal strain and inoculum type but inducers had no effect. The maximum yields obtained were 1310 U/g dm for AMG using a spore suspension of A. niger CCT 3312 and 50.2 U/g dm for exo-PG production, using A. niger t0005/007-2 and fermented bran as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyloglucosidase; Exo-polygalacturonase; Rice-bran; Solid-state fermentation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500003

Species Diversity and Community Structure of Arthropod Pests and Predators in Flax, Linum Usitatissimum L. from Darjeeling (India)
Provedor de dados: 52
Autores: Pal,Suprakash; Mandal,Rupsanatan; Sarkar,Indrajit; Ghimiray,Tulsi Saran; Sharma,Binay Raj; Roy,Ayon; Roy,Suvendu Kumar; Chakraboorty,Gautam; Mitra,Sabyasachi.
ABSTRACT The species richness and relative abundances of different aboveground plant-dwelling pest and predator species were assessed in flax (Linum usitatissimum L.) ecosystem under mid-hill conditions of eastern Himalayas. The experiment was conducted in winter seasons during 2004-2013 at the Regional Research Station (Hill Zone), Uttar Banga Krishi Viswavidyalaya, Kalimpong, West Bengal, India. Twenty five species of phytophagous arthropods under 13 families were observed belonging to 6 different orders. The gram pod borer, Helicoverpa armigera (Noctuidae: Lepidoptera), aphid, Myzus persicae (Aphididae: Hemiptera), shield bug, Plautia fimbriata (Pentatomidae: Hemiptera) and leaf webber, Nacoleia sp. (Pyralidae: Lepidoptera) were found as the most common...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flax; Pests; Predators; Species richness; Abundance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100459

Brassinosteroids Reciprocates Heavy Metals Induced Oxidative Stress in Radish by Regulating the Expression of Key Antioxidant Enzyme Genes
Provedor de dados: 52
Autores: Sharma,Indu; Sharma,Ashutosh; Pati,Pratap; Bhardwaj,Renu.
ABSTRACT Heavy metal toxicity in plants lead to accumulation of reactive oxygen species (ROS). Antioxidant enzyme system is also not able to revert altered ROS homeostasis. The present study reports the heavy metal induced ROS toxicity by up-regulating the expression of key antioxidant enzyme genes through Brassinosteroids pre-soaking treatment in radish.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassinosteroids; Oxidative stress; Antioxidant enzymes; Gene expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100425

The influence of techniques of larvae rearing and seed collectors on the survival rate and recovery efficiency of the brown mussel Perna perna (L.) in laboratory
Provedor de dados: 52
Autores: Beduschi,Pedro; Melo,Cláudio Manoel Rodrigues de; Ferreira,Jaime Fernando.
Te study compared the hatchery recovery efficiency and survival rate of Perna perna spats using larvae submitted to four conditions, prior to settlement, tested with for different collectors. In 15 days old larvae (eyed larvae, T1), transparent nylon thread collectors presented the best results for spats recovered per meter of collector. The 28 days old larvae (foot larvae) stored under refrigeration before settlement (T3 with water and T4 without water) showed no significant differences between the spat number in all the collectors. The brown multi-thread collector was more efficient in T 4. The blue polyamide thread collector was the most efficient with 28 days old larvae settled directly, without refrigeration (T2). Treatment 1 showed the highest spats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spats; Settlement; Perna perna; Collectors; Hatchery.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100019

Investigation of risk factors to coronary heart disease in two countryside villages
Provedor de dados: 52
Autores: Silva,Gisleine Elisa Cavalcante da; Bazotte,Roberto Barbosa; Curi,Rui; Silva,Maria Angélica Rafaini Covas Pereira da.
The aim of this study was to investigate risk factors to coronary heart disease in two countryside villages in the State of Paraná, southern Brazil, typical in terms of the poverty and lack of access to public health care. All population was invited to participate in the study. The 462 volunteers (61.3% female, mean age = 42 years) showed high prevalence of hypercholesterolemia (7.0%), hypertriglyceridemia (19.0%), hyperglycemia (11.0%), hypertension (30.0%) and obesity (16.0%). The majority of the individuals, during the interview did not report the diseases detected by the testes. The results revealed that the high prevalence of hypercholesterolemia, hypertriglyceridemia, obesity, hypertension and diabetes mellitus could be consequence of the delayed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperlipidemia; Hyperglycemia; Hypertension; Obesity; Coronary heart disease - Paraná State - Brazil.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300008

Effect of probiotic and prebiotic inclusion in weaned piglet diets on structure and ultra-structure of small intestine
Provedor de dados: 52
Autores: Budiño,Fábio Enrique Lemos; Thomaz,Maria Cristina; Kronka,Rodolfo Nascimento; Nakaghi,Laura Satiko Okada; Tucci,Fernanda Marcussi; Fraga,Alessandro Luís; Scandolera,Antônio João; Huaynate,Rizal Alcides Robles.
Forty-four piglets weaned were distributed in a complete randomized blocks in factorial scheme. Experimental diets were: T1 = Basal Diet (BD); T2 = BD + Antibiotic; T3 = BD + Probiotic; T4 = BD + Prebiotic; T5 = BD + Symbiotic (T3+T4). As control, four piglets were slaughtered at weaning. Seven and 14 days after weaning, four animals per treatment were slaughtered. Small intestine segments were sampled from each animal for electron and light microscopy evaluation. The evaluated micro-ingredients affected intestinal histology. It was observed higher villous density in duodenum of piglets fed diet with prebiotic in relation to those fed diets with probiotic. In jejunum of piglets fed diets with prebiotic was found higher villous density, but this difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crypts; Fructo-oligosaccharides; Micro-villi; Swine; Villi.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800008

Frequency of class I anti-HLA alloantibodies in patients infected by HIV-1
Provedor de dados: 52
Autores: Leite,Elza Regina Manzolli; Lima,Oswaldo Luiz Luz; Leite,Fábio Renato Manzolli; Costa,Paulo Inácio da.
The aim of this study was to evaluate the presence of class I anti-HLA alloantibodies in patients infected by HIV-1 and relate it with the different clinical courses of the disease. Blood samples were collected in EDTA tubes from 145 individuals. HIV-1 infection was confirmed by ELISA test. The presence of class I anti-HLA alloantibodies and HLA allele's were determined. Clinical evolution was set as fast (&lt;1 year between diagnostic and death), moderate (1-3 years) or slow (&gt;3 years). Class I anti-HLA alloantibodies presence was lower in healthy individuals than in those infected by HIV-1 (4.2% against 32.4%). However, an equal distribution of these alloantibodies was found among the individuals infected, independent on the clinical evolution. Thus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIDS; HIV; HLA antigens; Alloantibodies.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100012

Evaluation of arsenic and selenium in Brazilian soluble coffee by inductively coupled plasma atomic emission spectrometry with hydride generation
Provedor de dados: 52
Autores: Santos,Éder José dos; Oliveira,Elisabeth de.
A method for the evaluation of arsenic and selenium in soluble coffee by inductively coupled plasma atomic emission spectrometry with continuous hydride generation to attend the Brazilian food legislation is described. Samples were digested with nitric acid and hydrogen peroxide in a focused microwave system. Slow heating eliminated nitric acid and selenium (VI) was reduced to selenium (IV) by addition of 6 mol/L hydrochloric acid and heating at 90° C under a reflux system. The influence of sample acidity on sensitivity was investigated. Hydrochloric acid 6 mol/L was the most suitable reaction medium. Practical detection limits of 2.0mug/L for As and 1.0mu g/L for Se were achieved and attended the Brazilian food legislation. The results of recoveries on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ICP-AES; As; Se; Soluble coffee.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300003

Incidences of poisonings due to Chlorophyllum molybdites in the state of Paraná, Brazil
Provedor de dados: 52
Autores: Meijer,André August Remi de; Amazonas,Maria Angela Lopes de Almeida; Rubio,Giselia Burigo Guimarães; Curial,Rafael Martinez.
Three recent cases of poisoning by Chlorophyllum molybdites, including the first one known from Brazil, have been reported from the state of Paraná. A morphological description of the material causing the first poisoning was provided and the associated case history has been described in detail. An overview of this species' distribution in Brazil is given.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorophyllum; Poisonous fungi; Mycetism; Paraná (state) - Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300014

Growth of Nile tilapia Oreochromis niloticus fed diets with different levels of proteins of yeast
Provedor de dados: 52
Autores: Medri,Vandir; Medri,Waldir; Caetano Filho,Mauro.
This experiment was based on observations of 72 juveniles of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial mean weight of 37.27 ± 4.92g, distributed in 12 cages of 100 l to evaluate the effects of the yeast inclusion as proteins source in the diet. The fishes were distributed in a completely randomized design with four treatments (0; 20; 40; and 60%) of yeast protein in substitution to the protein of traditional sources with three repetitions. Effects of the treatments were not observed (p &gt; 0.05) on the survival and to food conversion. It was observed a quadratic effect on weight gain (Y = 73.39 + 0.173X - 0.0034X²; R²= 0.9986). It was concluded the best level of yeast inclusion as source proteins in the diet for reversed Nile...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Cages; Tilapia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300024

Effects of some phenolic compounds on soybean seed germination and on seed-borne fungi
Provedor de dados: 52
Autores: Colpas,Flávia Tavares; Ono,Elizabeth Orika; Rodrigues,João Domingos; Passos,José Raimundo de Souza.
Studies were carried out on the effects of coumarin, ferulic acid and naringenin on soybean seed germination and on the growth of seed-borne fungi at concentrations of 50 and 100 mg.L-1. The compounds showed good inhibition of seed germination, especially at 50 mg.L-1, but little fungistatic activity. Possible mechanisms of action are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Soybean; Phenolics; Coumarin; Ferulic acid; Naringenin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200003

Comparison between the SPD and the SPDS methods for segregating generation advancement in soybean
Provedor de dados: 52
Autores: Destro,Deonisio; Bizeti,Henrique Stoco; Garcia,Leandro Anderlin; Fonseca,Inês Cristina de Batista; Montalván,Ricardo; Miglioranza,Édison.
The purpose of this work was to compare the SPD (Single Pod Descent) and SPDS (Single Pod Descent with Selection) methods for segregating generation advancement in soybean breeding. Sixteen populations derived from crosses among Japanese and adapted Brazilian soybean were used. The traits, number of days to flowering (NDF) and to maturity (NDM), plant height at flowering (PHF) and at maturity (PHM), and individual plant yield (IPY) were assessed. The populations obtained by SPDS showed values close to the variation in the populations obtained by SPD. Regression through the origin showed that both methods were equivalent for NDM in the F3 generation and for NDF in the F4 generation. For all other trait/generation combinations, SPDS yielded superior means....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Breeding methods; Food type soybean.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400008

Rhamnolipid Biosurfactants Produced by  Pseudomonas Species
Provedor de dados: 52
Autores: Kaskatepe,Banu; Yildiz,Sulhiye.
ABSTRACT: Surfactants are chemical products widely used in our daily life in toothpaste and other personal hygiene and cosmetic products, and in several industries. Biosurfactants are surfactants of biological origin that can be produced by microorganisms and have many advantages, such as low toxicity and high biodegradability, compared to synthetic counterparts. Unfortunately, high production costs limit the use of biosurfactants. Low-cost production is the most important factor for biosurfactants to be able to compete in the global market place. This review presents general information on rhamnolipid biosurfactant produced by Pseudomonas species, as well as on their production and applications. In addition, industrial products and their wastes used for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Application of rhamnolipid; Biosurfactant; Pseudomonas spp.; Rhamnolipid.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100420

Natural diet of three species of shrimp in a tropical coastal lagoon
Provedor de dados: 52
Autores: Albertoni,Edélti Faria; Palma-Silva,Cleber; Esteves,Francisco de Assis.
The gut content of 495 specimens of Farfantepenaeus brasiliensis, 131 of F. paulensis (Penaeidae) and 102 of Macrobrachium acanthurus (Palaemonidae) were analyzed to establish the composition of their diets F. brasiliensis had as the most important feeding items in its diet larvae of Chironomidae, Polychaeta and Heleobia australis (Mollusca). For F. paulensis, the most important items were the same as for F. brasiliensis, but the order of importance of H. australis and Polychaeta was inverted. M. acanthurus had detritus as the most important item, followed by Chironomidae larvae, Odonata nymphs, and fragments of the macroalgae Chara. The results showed that the three species were omnivorous, with a varied diet including both components of macrofauna of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal lagoon; Feeding; Farfantepenaeus brasiliensis; Farfantepenaeus paulensis; Macrobrachium acanthurus; Feeding Index.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300011

Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents
Provedor de dados: 52
Autores: Diniz,Claudia Leite; Carmo,Fernanda Santos do; Almeida,Danielle Santos de; Santos-Filho,Sebastião David; Missailidis,Sotiris; Fonseca,Adenilson de Souza da; Bernardo Filho,Mario.
This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p&lt;0.05) the %ATI/g in several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodistribution; Centella asiatica; Blood; Plasma; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700035

Genetic control on morphoagzronomic traits in snap bean
Provedor de dados: 52
Autores: Silva,Marlon Peres da; Amaral Júnior,Antônio Teixeira do; Rodrigues,Rosana; Pereira,Messias Gonzaga; Viana,Alexandre Pio.
Five divergent snap bean accessions from the vegetables germplasm bank at UENF and their ten diallel hybrids were evaluated to inheritance on five morphoagronomic traits using Hayman's methodology (1954). The results showed that additive effects were predominant for pod weight per plant, number of seeds per pod, height of the insertion of the first pod and number of days to flowering while non-additive effects were more important for number of pods per plant. The best strategy to be adopted was the use of these acessions in an intrapopulation breeding program aiming the obtaintion of superior segregants. For pod numbers the indicated strategy would be an interpopulational breeding procedure, to exploite the heterosis related to the non-additive effects....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snap bean; Phaseolus vulgaris; Diallel analysis; Inheritance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600004

Length-weight relationships for five cladoceran species in an Amazonian lake
Provedor de dados: 52
Autores: Maia-Barbosa,Paulina Maria; Bozelli,Reinaldo Luiz.
This work shows the length-weight regressions established for the five most frequent and abundant cladoceran species (Bosmina hagmanni, Bosminopsis deitersi, Ceriodaphnia cornuta, Diaphanosoma birgei, and Moina minuta) in two stations at Lake Batata. This lake has received for 10 years very fine bauxite processing tailings, which is responsible for the thick layer over the natural sediment still observed in its western section (impacted station). The individuals were separated into size classes, the dry weight values were determined for each class and were used for establishing weight-length equations. Bosminopsis deitersi and Ceriodaphnia cornuta were the species showing the smallest size in the two sampled areas (0.152 to 0.322 mm) and Diaphanosoma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian lake; Zooplankton; Cladoceran; Length-weight relationships.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200018

Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant
Provedor de dados: 52
Autores: Sharmin,Shamima Akhtar; Alam,Md. Jahangir; Sheikh,Md. Mominul Islam; Sarker,Kanak Kanti; Khalekuzzaman,Muhammad; Haque,Md. Anwarul; Alam,Mohammad Firoz; Alam,Iftekhar.
In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro; Medicinal plant; Micropropagation; Somatic embryo; Threatened; Wedelia calendulacea.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013

Constitution and composition chemistry of the precooked goatlike buchada produced in the state of Paraíba, Brazil
Provedor de dados: 52
Autores: Santos,Neube Michel dos; Costa,Roberto Germano; Madruga,Marta Suely; Medeiros,Ariosvaldo Nunes de; Albuquerque,Caroline Lima Cavalcante de; Queiroga,Rita de Cássia Ramos do Egypto.
The objective of the present work was to quantify the constitution and composition chemistry of the precooked goat buchada produced in the state of Paraíba, Brazil. The goat buchada presented considerable yield in relation to the live weight. High protein content was observed in the products analyzed. The lipid content presented high variation as result of the lack of standardization of products produced in the state and no control on parameters such as age, weight and breed of the slaughtered animals was observed. Although no difference (P&lt;0.05) in the lipid content was observed, the highest percentages were observed in samples from Remígio and Barra de Santa Rosa, with 14.52 and 15.90%, respectively, as result of the addition of high amounts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: "buchada" yield; Fat; Organs; Protein; Viscera.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400017

Effects of organic fertilizers on the growth and yield of bush bean, winged bean and yard long bean
Provedor de dados: 52
Autores: Islam,Mohammad Aminul; Boyce,Amru Nasrulhaq; Rahman,Md Motior; Azirun,Mohd Sofian; Ashraf,Muhammad Aqeel.
ABSTRACT VC (20%), TC (20%) and N:P:K fertilizer (farmer's practice) were used to determine the growth and yield attributes of bush bean (Phaseolus vulgaris), winged bean (Psophocarpus tetragonolobus) and yard long bean (Vigna unguiculata). Plants grown with VC (20%) produced the highest fresh biomass for bush bean (527.55 g m-2), winged bean (1168.61 g m-2) and yard long bean (409.84 g m-2). In all the tested legumes the highest pod weight, pod number, pod dry weight and pod length were found in the VC (20%) treatment. Photosynthetic rates in the three legumes peaked at pod formation stage in all treatments, with the highest photosynthetic rate observed in winged bean (56.17 µmol m-2s-1) grown with VC (20%). The highest yield for bush bean (2.98 ton...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vermicompost; Compost; Phaseolus vulgaris; Psophocarpus tetragonolobus and Vigna unguiculata; Photosynthesis; Protein content.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200205

Amplification of the cap20 pathogenicity gene and genetic characterization using different markers molecular in Colletotrichum gloeosporioides isolates
Provedor de dados: 52
Autores: Maciel,Danielli Barreto; Medeiros,Lílian Vieira de; Medeiros,Vivian Vieira de; Leão,Mariele Porto Carneiro; Camargo,Luis Eduardo Aranha; Oliveira,Neiva Tinti de.
Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative to separate the Colletotrichum sp, and that primer EI1 demonstrated greater genetic diversity. The amplification of the DNA of the Colletotrichum isolates to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cap20 gene; Colletotrichum gloeosporioides; Pathogenicity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600001

Ultrasound efficiency in relation to sodium hypochlorite and filtration adsorption in microbial elimination in a water treatment plant
Provedor de dados: 52
Autores: Bello,Antonio Roberto Crystal; Angelis,Dejanira de Franceschi; Domingos,Roberto Naves.
Processes like ultrasound, chlorination and filtration-adsorption were compared to eliminate microorganisms and to adjust established parameters of public drinking water. A mini water treatment plant (WTP-CB), in pilot scale, was projected and built to evaluate each process influence as: decontamination, coagulation-flocculation, sedimentation and filtration. Total and fecal coliforms, Escherichia coli and heterotrophic bacteria and physic/chemical parameters were quantified from water. Ultrasound, chlorination and filtration-adsorption were efficient to inactivate and/or eliminate bacteria. Ultrasound decontamination in addition to coagulation-flocculation, sedimentation and filtration, could be considered as an alternative treatment water solution where...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water; Filtration; Microorganisms; Ultrasound; Chlorination.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600009

Movement of southern elephant seals (Mirounga leonina L.) from Elephant Is. South Shetlands, Antarctica
Provedor de dados: 52
Autores: Muelbert,Mônica M. C.; Robaldo,Ricardo B.; Martínes,Pablo E.; Colares,Elton P.; Bianchini,Adalto; Setzer,Alberto.
In 1999, at-sea activity of two young southern elephant seal males (Mirounga leonina) from Elephant Is. (61º13'S, 55º23'W), Antarctica, was monitored and tracked for 9 months. The individuals were randomly selected, captured, sedated (Zoletil 50®- 1mg/kg), weighed, measured, bled, paint-marked and fitted with satellite tags (STDR - ST-6PPT, Telonics®, USA). Deployment of the STDR took about 45 min since each animal had a lower incisor tooth extracted for age determination. The seals exhibited individual behaviors. Seal "V"-23842 (BM ~ 801kg) moved from Elephant Is. (61.2ºS 55.3ºW) in Jan. 1999 to King George Is. (62.2ºS 58.1ºW) in Feb. 1999 when the tag stopped signaling. Seal "T"-23843 (BM ~ 656 kg) was restricted to the area around Elephant Is. (61.2ºS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mirounga leonina; Southern elephant seals; Satellite telemetry; Antarctica; At-sea activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300017

Inactivation of Salmonella enteritidis on raw poultry using microwave heating
Provedor de dados: 52
Autores: Pucciarelli,Amanda B.; Benassi,Fernando O.
The effect of microwave heating on Salmonella Enteritidis inoculated on fresh chicken was investigated using a microwave oven (800 w) to determine the destruction of Salmonella Enteritidis isolated from chicken carcasses, in relation to the time of heating at two power settings: high (power level 10) and medium (power level 6); The relationship between heating time and temperature was also been studied. The destruction was 6.4 log cycles at time 95 sec for the high power level, and 5 log cycles at time 140 sec for medium power setting. After 110 sec for higher power level, no survival of Salmonella Enteritidis was detected in samples (100g), but at 140 sec for medium power level, these food pathogens were still present.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microwave; Salmonella enteritidis; Destruction; Chicken thighs.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800010

Phytochemical and Morphological Evidences for Shikonin Production by Plant Cell Cultures of Onosma sericeum Willd
Provedor de dados: 52
Autores: Gharehmatrossian,Sormeh; Popov,Yu; Ghorbanli,Mahlagha; Safaeian,Shila; Iranbakhsh,Alireza.
ABSTRACT Shoot regeneration, callus growth, and biosynthesis of shikonin in callus cultures of Onosma sericeum were examined. Plant tissue culture was used as an alternative method for increasing the production of shikonin, a secondary metabolite. The isolated cultures were subjected to abiotic factors such as light, plant growth regulators, and nutritional factors. Identification was carried out by High- Performance Liquid Chromatography (HPLC) after 10th subculture. Nodal explants were incubated in Murashige and Skoog (MS) medium along with different combination of growth hormones. Shoot regeneration from calli were achieved on MS basal medium supplemented with 3 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l Naphthalene acetic acid (NAA) under light cycle....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth index; Medicinal plant; Plant regeneration; Secondary metabolite.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100428

Influences of Different Coating Materials on the Quality Changes of Hardboiled Salted Duck Eggs under Ambient Storage
Provedor de dados: 52
Autores: Venkatachalam,Karthikeyan; Lekjing,Somwang; Noonim,Paramee.
Abstract The present study investigated the influences of selected coatings (paraffin wax (PW), chitosan (CH), whey protein isolate (WPI), and soy protein isolate (SPI)) on the quality changes of hardboiled salted duck eggs when kept under ambient temperature (30±2 oC). At 5-day intervals for 15 days, samples were tested for color (L*, C*, and h˚), shell strength, weight loss, microbial analysis, water activity, moisture, pH, salt content, TBARS, and sensory analysis. L*, C* and h˚ gradually decreased in egg white, whereas C* and h˚ gradually increased in egg yolk. Shell strength gradually decreased in all cases, and weight loss similarly increased throughout the storage. PW and WPI coatings gave the best shell strengths and the least weight loss. The aw...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duck egg; Salting; Coating; Storage; Temperature; Quality.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100512

Evaluation of emulsifier stability of biosurfactant produced by Saccharomyces lipolytica CCT-0913
Provedor de dados: 52
Autores: Lima,Álvaro Silva; Alegre,Ranulfo Monte.
Surface-active compounds of biological origin are widely used for many industries (cosmetic, food, petrochemical). The Saccharomyces lipolytica CCT-0913 was able to grow and produce a biosurfactant on 5% (v/v) diesel-oil at pH 5.0 and 32ºC. The cell-free broth emulsified and stabilized the oil-in-water emulsion through a first order kinetics. The results showed that the initial pH value and temperature influenced the emulsifier stability (ES), which was the time when oil was separated. The biosurfactant presented different stabilization properties for vegetable and mineral oil in water solution, despite the highest values of the ES occurring with vegetable oil. The biosurfactant presented smallest ES when compared to commercial surfactants; however, this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactant; Emulsifier stability; Fermentation; Vegetable oil; Diesel oil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200004

Gills scanning images of the seawater fish Eugerres brasilianus (Gerreidae)
Provedor de dados: 52
Autores: Eiras-Stofella,Daura Regina; Charvet-Almeida,Patricia.
The gills of the adult fish, Eugerres brasilianus (Gerreidae) were analyzed in a scanning electron microscope. The stratified epithelium was uniform on all parts of the branchial arch. Concentric microridges were present on cells that form this epithelium and were mainly observed in the primary lamellae and pharyngeal region where mucous cells were also abundant. The ultrastructural features of E. brasilianus gills indicated that this was not a filtering species, and that the feeding habit included mainly the intake of small organisms. The results presently obtained agreed with other literature data which determined the feeding habit of this species by means of stomach content analysis and other aspects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gill; Morphology; Scanning; Eugerres brasilianus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400011

Heavy Metals in Garra gotyla , Cyprinus carpio and Cyprinion watsoni from the River Panjkora, District, Lower Dir, Khyber Pakhtunkhwa, Pakistan
Provedor de dados: 52
Autores: Ullah,Sana; Hussain,Zaqhim; Mahboob,Shahid; Al-Ghanim,Khalid.
ABSTRACT This study was conducted in the River Panjkora, Pakistan, in order to assess the level of heavy metals accumulated in the liver, kidneys, gills and muscle tissues of three highly consumed fish species by the local community, Garra gotyla, Cyprinus carpio and Cyprinion watsoni. The heavy metals including manganese (Mn), iron (Fe), chromium (Cr), zinc (Zn), lead (Pb), cadmium (Cd) and nickel (Ni) were determined in the collected fish specimens through an atomic absorption spectrophotometer. Level of heavy metals significantly varied depending upon tissues type and fish species. Cyprinus carpio accumulated highest concentration of heavy metals, followed by Garra gotyla while Cyprinion watsoni accumulated least concentration of heavy metals. All the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: River; Control site; Tissues; Pollution; Heavy Metals.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100702

Solar Energy for Residential Use and Its Contribution to the Energy Matrix of the State of Paraná
Provedor de dados: 52
Autores: Garcia,Gabriela; Nogueira,Eliel Ferreira; Betini,Roberto Cesar.
ABSTRACT The present study evaluates the economic viability of the application of solar energy for electric power generation via the use of photovoltaic systems in a residential consumption unit in the city of Curitiba. Since the energy from the sun is abundant, clean, renewable and has the potential to compete in productivity and profitability, the evaluation of the applicability of these systems in homes, not only in industrial parks, is of great interest. A household with the determined consumption profile was chosen for this case study through simulations with the HomerPro software. After analyzing the data, the photovoltaic potential of the State of Paraná was estimated to investigate the possibility of photovoltaic generation growth in the state...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic Systems; Solar Energy; Renewable Energy; Photovoltaic Power Generation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200211

Zinc detoxification by a cyanobacterium from a metal contaminated bay in Brazil
Provedor de dados: 52
Autores: Andrade,Luciana; Keim,Carolina N.; Farina,Marcos; Pfeiffer,Wolfgang C.
We describe here the trapping of zinc in polyphosphate granules of the cyanobacterium Synechocystis aquatilis NPBS-3. Cells were cultured in 25 µM of zinc chloride and prepared for electron microscopy and energy-dispersive X-ray analysis. Some ultrastructural features were changed by zinc exposure, the increase of glycogen granules number being the main change. The polyphosphate granules contained phosphorus, sulphur, calcium, iron and zinc. The trapping of zinc in polyphosphate granules seemed to be an effective way of detoxifying the metal and surviving in the bay. As a non-specific mechanism, these polyphosphate granules could also be effective in trapping other metals in excess.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metals; Zinc; Sepetiba Bay; Polyphosphate granules; Glycogen granules; Detoxification.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100020

Use of the burrowing amphipod Tiburonella viscana as a tool in marine sediments contamination assessment
Provedor de dados: 52
Autores: Abessa,Denis M.S.; Sousa,Eduinety C.P.M.; Rachid,Bauer R.F.; Mastroti,Ricardo R.
The acute toxicity of sediment samples from Santos Estuary was evaluated by using sediment toxicity tests with the burrowing amphipod Tiburonella viscana. Survival of amphipods acclimated for 2 and 15 days was also analyzed. The sensitivity of this species was evaluated by 48h-exposure tests using potassium dichromate (K2Cr2O7) as a reference substance. The sediment samples from Gonzaga Beach, Prainha and Paranapuã Beach were not toxic to T. viscana, whereas the samples collected at Santos Channel and São Vicente Bay were toxic. The acclimation period did not affect survival significantly, in spite of the higher mortality within the 15-day acclimated group. The estimated 48-h LC50 to K2Cr2O7 was 6.029 mg/L (3.27-11.12). Toxicity tests using T. viscana were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphipoda; Tiburonella viscana; Toxicity test; Sediment.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200009

Use of bee-attractants in increasing crop productivity in Niger (Guizotia abyssinica. L)
Provedor de dados: 52
Autores: Sivaram,Venkataramegowda; Jayaramappa,Koragandahalli Venkateshappa; Menon,Anita; Ceballos,Ruben Michael.
The use of bee attractants, Bee-Q and Fruit BoostTM in the pollination of niger was evaluated. Bee visitations to niger flowers were observed for two weeks and an estimation of seed yield was determined. Different concentrations of Bee-Q and Fruit boostTM were evaluated to understand honeybee visitation patterns on the target crop and pollination efficiency. Results indicate that applications of Bee-Q at 12.5 g/l and Fruit boost at 0.75 ml/l on niger plots significantly increased the number of bee foragers over control plots. In addition, plots sprayed with these bee attractants significantly enhanced the seed set, seed weight, and germination of niger. This study suggests that pheromone-based bee attractants applied to niger can increase the marginal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bee-attractants; Honeybee; Guizotia abyssinica; Niger; Pollinators.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300003

Xylitol production by Candida parapsilosis under fed-batch culture
Provedor de dados: 52
Autores: Furlan,Sandra A.; Castro,Heizir F. de.
Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates. The presence of glucose in the medium induced the production of ethanol as secondary metabolite and improved specific rates of growth, xylitol formation and substrate consumption. Fractionated supply of the feed medium at constant sugar concentration did not promote any increase on the productivity compared to the single batch cultivation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida parapsilosis; Glucose; Xylose; Xylitol; Fed-batch.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200003

Neural network based control of an absorption column in the process of bioethanol production
Provedor de dados: 52
Autores: Eyng,Eduardo; Silva,Flávio Vasconcelos da; Palú,Fernando; Fileti,Ana Maria Frattini.
Gaseous ethanol may be recovered from the effluent gas mixture of the sugar cane fermentation process using a staged absorption column. In the present work, the development of a nonlinear controller, based on a neural network inverse model (ANN controller), was proposed and tested to manipulate the absorbent flow rate in order to control the residual ethanol concentration in the effluent gas phase. Simulation studies were carried out, in which a noise was applied to the ethanol concentration signals from the rigorous model. The ANN controller outperformed the dynamic matrix control (DMC) when step disturbances were imposed to the gas mixture composition. A security device, based on a conventional feedback algorithm, and a digital filter were added to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Absorption column; Artificial neural network; Feedforward control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400020

Technetium-99m nitrido radiopharmaceuticals with unprecedented biological properties
Provedor de dados: 52
Autores: Duatti,Adriano; Boschi,Alessandra; Uccelli,Licia.
The chemical methods for the production of technetium-99m radiopharmaceuticals containing a terminal Tc&lt;FONT FACE=Symbol&gt;º&lt;/FONT&gt;N triple bond have been established more than a decade ago. From that time, the chemistry of nitrido Tc-99m complexes has provided a highly efficient tool for the design and preparation of novel classes of diagnostic agents, and a number of potentially useful radiopharmaceuticals have been discovered. In particular, nitrido technetium-99m tracers have been developed for heart perfusion imaging. In this short review, the chemical and biological properties of the neutral myocardial perfusion tracer bis(N-ethoxy, N-ethyl-dithiocarbamato) nitrido Tc-99m (TcN-NOEt) will be summarized along with the preparation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m Radiopharmaceuticals; Nitrido Complexes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500019

Antimicrobial effectiveness of spices: an approach for use in food conservation systems
Provedor de dados: 52
Autores: Souza,Evandro Leite de; Stamford,Tânia Lúcia Montenegro; Lima,Edeltrudes de Oliveira; Trajano,Vinícius Nogueira; Barbosa Filho,José Maria.
There has been constant an increasing the search alternative and efficient compounds for food conservation, aiming a partial or total replacement of antimicrobial chemical additives. Spices offer a promising alternative for food safety. Inhibitory activity of spices and derivatives on the growth of bacteria, yeasts, fungi and microbial toxins synthesis has been well reported, so they could be used in food conservation as main or as adjuvant antimicrobial compounds in order to assure the production of microbiologically stable foods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spices; Essential oil; Extracts; Chemical compounds; Antibacterial activity; Antifungal activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500007

Macroinvertebrate fauna associated to the bromeliad Vriesea inflata of the Atlantic Forest (Paraná State, southern Brazil)
Provedor de dados: 52
Autores: Mestre,Luiz Augusto M.; Aranha,José Marcelo R.; Esper,Maria de Lourdes P.
The accumulated water inside the bromeliad leaf rosette is a microhabitat for several animal and plant species. In this study, the associated fauna of bromeliad Vriesea inflata was analyzed related to seasons, bromeliad height in relation to the ground and environmental factors. The samples were seasonally collected in Quatro Barras (a municipality of Paraná State, southern Brazil) between March 1996 and March 1997. The associated fauna was very rich and it was grouped in 23 taxonomic groups, with a dominance of Coleoptera Scirtidae, Diptera and Hymenoptera Formicidae. We found higher abundance of macroinvertebrates in terrestrial bromeliads during the spring/1996 and in epiphyte bromeliads in the autumn/1997.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliaceae; Atlantic Forest; Associated fauna.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100012

Determination of the Effect of the Elimination Diet Applied for Overweight and Obese People with Food Intolerance on Body Composition and Biochemical Parameters
Provedor de dados: 52
Autores: Gubur,Sema.
ABSTRACT   Food Intolerance, is a reaction against food, but not immunological manner, and may be confused with real food allergies. In this study, effects of special weight-loss diet together with an elimination diet on body composition and biochemical parameters of overweight and obese people who were diagnosed with food intolerance were investigated. The study group consists of 20 patients in total who were followed-up and treated in Yorktest Turkey Laboratory; who were diagnosed with food intolerance, and whose BMI was &gt; 26kg/m2. Bloodletting for these patients was executed with Lancet from their fingertips, and the blood drawn from these patients was assessed via enzyme-linked immunosorbent assay (ELİSA) method, and food reactions of patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food intolerance; Obesity; IgG; Elimination diet; Yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100305

Morphological, Chemical and Molecular Characterization of ‘Old olive’ (Olea europaea L.) from Montenegro
Provedor de dados: 52
Autores: Adakalic,Mirjana; Lazovic,Biljana.
ABSTRACT Thousands of years of tradition of olive cultivation in Montenegro is witnessed with the ‘Old olive’ estimated to be more than 2000 years old. ‘Old olive’ draws attention to its genetic potential and the relation to the closest grown olive cultivars. Therefore, the aim of this paper was to give the basic description of ‘Old olive’ (through 20 morphological and 37 chemical properties) as well as to determine its DNA profile and to examine the closeness with major olive cultivar grown, cv. ‘Žutica’. The obtained data showed the differences between examined samples. ‘Old olive’ started flowering (27. May) more than 10 days later than cv. ‘Žutica’ (14. May), and began ripening 15 days later (20th October and 5th October, respectively). From 21...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ‘Old olive’ tree; Morphology; Oil; SSR; Cv. ‘Žutica’; Montenegro.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100432

Intrauterine sexual differentiation: biosyntesis and action of sexual steroid hormones
Provedor de dados: 52
Autores: Santos,Amilton Cesar dos; Viana,Diego Carvalho; Oliveira,Gleidson Benevides de; Lobo,Luis Miguel; Assis-Neto,Antônio Chaves.
The objective of this review was to describe sexual differentiation events in mammals, relating them to biosynthesis of sexual steroid hormones and their mechanisms of action. Cholesterol is the precursor of sexual steroid hormone biosynthesis via action of several enzymes converting these hormones. Progestagens hormones serve as substrate for the production of androgens, which in turn serve as substrate for estrogen hormones. These hormones are responsible for sexual differentiation and reproductive cycles of mammals. Sexual differentiation process comprises determining the sexual chromosomes XX or XY + SRY and other genes linked to them, differentiation of gonads in testis or ovary, differentiation of internal and external male or female genital organs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P450 cytochrome; Sexual differentiation; Müllerian ducts; Wolff duct; Steroidogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300395

Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge
Provedor de dados: 52
Autores: Viancelli,Aline; Kunz,Airton; Esteves,Paulo Augusto; Bauermann,Fernando Viçosa; Furukawa,Kenji; Fujii,Takao; Antônio,Regina Vasconcellos; Vanotti,Matias.
The present study aimed to describe the bacterial community present at an anaerobic up flow bioreactor with ANAMMOX activity, inoculated with the sludge from the anaerobic pond of a swine slurry treatment system. The description was based on the molecular DNA techniques using primers for amplification of complete 16S rRNA gene and also new primers to amplify smaller fragments from 16S rRNA. During the bioreactor operation time, the bacterial community changed significantly, increasing the nitrogen removal efficiency, reaching after 500 days a removal rate of 94%. The complete PCR amplification of 16S rRNA gene generated 17 clones, where three presented similarity with Candidatus Jettenia asiatica (97%), twelve with Janthinobacterium (99%) and two with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ANAMMOX; Anaerobic pond; Sequencing; Swine manure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500022

Production of Cellulose and Profile Metabolites by Fermentation of Glycerol by Gluconacetobacter Xylinus
Provedor de dados: 52
Autores: Vidotto,Francielle Lina; Ribeiro,Geovana Piveta; Tischer,Cesar Augusto.
ABSTRACT   Because of the widespread occurrence of cellulose in nature, many organisms use glycerol as a source of carbon and energy, so these organisms have drawn attention to the potential use of glycerol bioconversion. The bacteria Gluconacetobacter xylinus, a strictly aerobic strain that performing incomplete oxidation of various sugars and alcohols to cellulose biosynthesis. For this reason, we modify the Hestrim-Schram medium, associating glycerol, glucose and sucrose varying their concentration. The fermentations were performed statically at a temperature of 28˚C for a period of 10 days. The pH, membrane formation, crystallinity and the production of some metabolites of the 4th, 7th and 10th days was evaluated. The results showed a higher yield of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial cellulose; Glycerol; Metabolites; Gluconacetobacter xylinus; Solid state nuclear magnetic resonance; 1H nuclear magnetic ressonance.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100404

Abiotic variables in littoral-limnetic gradient of an oxbow lake of Mogi-Guaçu River floodplain, Southeastern, Brazil
Provedor de dados: 52
Autores: Taniguchi,Glória Massae; Bicudo,Denise de Campos; Sennab,Pedro Américo Cabral.
The present study aimed to analyse the abiotic characteristics spatial variability in the littoral-limnetic gradient of the Diogo Pond, Mogi-Guaçu River floodplain, as well as evaluating the hydrological influence on the spatial gradient. During the hydrological cycle, four field trips (high water, flood, low water, and drought) were carried out at three sampling stations: littoral, interface, and limnetic region. Analysis of physical and chemical variables allowed to conclude that the time scale established by the hydrological cycle was the main forcing function over the limnological variability of the Diogo Pond. The relative spatial scale related to littoral-limnetic gradient contributed secondarily to the abiotic variability. Littoral was characterized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxbow lake; Littoral-limnetic gradient; Physical and chemical features.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600016

Temperature effects on Trichogramma pretiosum Riley and Trichogrammatoidea annulata De Santis
Provedor de dados: 52
Autores: Maceda,Arlei; Hohmann,Celso L; Santos,Honório R. dos.
The influence of temperature on lifetime attributes of Trichogramma pretiosum Riley and Trichogrammatoidea annulata De Santis (Hymenoptera: Trichogrammatidae) was evaluated at four constant temperatures (15, 20, 25, and 30º C), RH 70 ± 10%, photophase 14 h. Anagasta kuehniella (Lepidoptera: Pyralidae) eggs were used as hosts. Developmental times of both parasitoid species were similar when exposed to 20, 25, or 30º C. T. annulata, however, developed slightly faster than T. pretiosum at 15º C. Emergence rates of both species were above 89%. The temperature threshold for T. pretiosum and T. annulata was 11º C and the number of degree-days required for their development was 126.9 and 122.3, respectively. Parasitization was maximal at 25º C. T. annulata,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Biological control; Avocado fruit borer; Temperature.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100005

Natural diet of Neohelice granulata (Dana, 1851) (Crustacea, Varunidae) in two salt marshes of the estuarine region of the Lagoa dos Patos lagoon
Provedor de dados: 52
Autores: Barutot,Roberta Araujo; D'Incao,Fernando; Fonseca,Duane Barros.
Natural diet of Neohelice granulata in two salt marshes of Lagoa dos Patos, RS were studied. Sampling was performed seasonally and crabs were captured by hand by three persons during one hour, fixed in formaldehyde (4%) during 24 h, transferred to alcohol (70%). Each foregut was weighed and repletion level was determined. Differences between sexes in the frequencies of occurrence of items were tested by χ2test. A total of 452 guts were analyzed. Quali-quantitative analyses were calculated following the method of relative frequency occurrence and relative frequency of the points. At both sites, for both sexes and in all seasons, the main food items were sediment, Spartina sp. and plant detritus. The highest values of mean repletion index were estimated for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea; Brachyura; Diet; Salt marsh.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100012

Metastasic bone pain management with radioactive isotopes
Provedor de dados: 52
Autores: Coya Viña,Juan.
Pain is the commonest clinical manifestation of bone metastases. Its treatment is palliative in nature, and consists of chemotherapy, radiotherapy, hormonotherapy, diphosphonates, and drug therapy (i. e., opiates). Radioactive isotopes represent an appealing alternative to conventional treatment modalities. Among the different types of isotopes, wide clinical experience with 153Sm has been obtained in this laboratory. In the present study, 94 patients (mean age = 65 years), who had been diagnosed of having breast, prostate and other malignant tumors, were evaluated. These patients were treated with 37 MBq/Kg 153Sm-EDTMP. All of them complained of bone pain and had scintigraphic evidence of metastatic bone dissemination. Treatment efficacy was evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metastasic bone; 153Sm-EDTMP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700018

Insulin or insulin-like studies on unicellular organisms: a review
Provedor de dados: 52
Autores: Souza,Alzira Martins Ferreira de; López,Jorge A.
This paper presents a review of studies about insulin and insulin-like substances in prokaryotes, eukaryotes and fungi have been published over the last two decades, constituting an updating of our previous review (1988) which included references to invertebrate insulin or insulin-like substances both in uni- and pluricellular Monera, Protoctista, Fungii and Animal species. This present article reviews experiments and evidence obtained using modern techniques in the understanding of molecule evolution and behaviour, which confirm its very ancient molecular structure origin. The involvement of insulin-like and related material in signalling biological pathway and modulator effects is also reported.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Unicellular; Invertebrate; Insulin; Insulin-like; Insulin receptor.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600017

Reproductive biology of a tetragonopterinae (osteichthyes, characidae) of the Ubatiba fluvial system, Maricá - RJ
Provedor de dados: 52
Autores: Mazzoni,Rosana; Petito,Jaqueline.
Aspects of the reproductive biology of one Tetragonopterinae was assessed in the Ubatiba river - Maricá / RJ. Length structure and mean size suggested sexual dimorphism, females reaching larger sizes than males. The onset of sexual maturity occured at 2.8cm for both the sexes. Fecundity, determined on ripe ovaries, showed positive correlation to females size and weight and varied from 595 to 5520 for 3.9cm and 6.2cm lengths respectively. The spawning type, defined as total, was determined by the oocyte diameter frequency from ovaries in different development stages. The frequency distribution of ovarian/testis maturation stages, associated with the temporal variation of the gonadosomatic index, suggested that the reproduction period was prolonged along the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetragonopterinae; Coastal stream; Reproductive biology.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400011

Clinical evaluation of pearl millet conophor weaning mix as supplementary food for Nigerian children
Provedor de dados: 52
Autores: Akeredolu,I. A.; Addo,A. A.; Akeredolu,O. A.
The purpose of this study was to formulate a weaning diet from pearl millet-conophor nut flour that would promote growth. For PER, BV, NPU and TD values, casein diet was the most superior while millet-conophor diet and soy-ogi diet compared favourably with each other. For the clinical measurements of the experimental rats on the soy -ogi diet and millet-conophor diet, apart from the urinary urea level of the group on millet conophor diet which was much higher than soy-ogi, there was no difference in any of the other parameters measured. It was therefore, concluded that the millet-conophor, diet was favourably well with the soy-ogi.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical Evaluation; Conophor; Weaning Mix; Millet and Supplementary Food.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500004

Water osmotic absorption in Coleus blumei plants under salinity stress
Provedor de dados: 52
Autores: Sena,José Ozinaldo Alves de; Zaidan,Humberto Actis; Castro,Paulo Roberto de Camargo e.
Three month old Coleus blumei plants in pots were treated with different NaCl concentrations: 0.00, 0.25, 0.50 and 1.00%. To determine the water osmotic absorption, the plants had their stems cut at 10 cm from the soil surface. The remaining stems were linked to glass tubes by flexible rubber tubes. Readings of the water column level in the glass tubes were performed at each 30 minutes, corresponding to the water osmotic absorption, with a total of eleven readings. Other Coleus blumei, with the same age, received the NaCl concentrations, and were evaluated under field conditions in terms of transpiration and stomatal resistance. A randomized complete block analysis was used with five replications. An increase of osmotic absorption was verified for all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water relations; Water potential; Transpiration; Stomatal resistance.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700001

Phytosociological studies on natural establishment of vegetation in an unreclaimed limestone mining
Provedor de dados: 52
Autores: Frenedozo-Soave,Rita de Cássia.
Phytosociological structure was studied in limestone mining quarries at Rio Claro, São Paulo, Brazil. Quarries presented a chronosequence, ranging 1 to 40 years old, with focus on vegetation community organization, floristic similarity and plant diversity of three areas in different successive stages, devastated by limestone mining. A total of 1957 individuals distributed in 32 botanical families and 91 species were sampled. Low species diversity was obtained, ranged among quarries ages. In the early-established quarries, Leguminosae, Malvaceae and Sterculiaceae were the families most representative in number of species, while for the age 27, Asteraceae and Poaceae were the most representative. Diversity indices indicated that species diversity changed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Phytosociology; Mining quarry; Limestone mine; Spoil banks; Floristic.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200018

Growth and development of Mentha piperita L. in nutrient solution as affected by rates of potassium
Provedor de dados: 52
Autores: Valmorbida,Janice; Boaro,Carmen Sílvia Fernandes.
The influence of potassium on the development of Mentha piperita L. was evaluated in the plants grown in nutritive solution modified by variations in potassium, at 6.0, 3.0, and 1.5 mmol L-1, establishing differences between treatments and corresponding, respectively, to the concentration proposed in Hoagland and Arnon's no. 2 solution and reductions by 50% and 75%. Until 21 days after transplanting, the concentration used was diluted to 50% in the three treatments. Evaluations consisted of leaf area, development of the above-ground part, and dry matter of different plant organs. Plants grown with 1.5/3.0 mmol L-1 K showed greater development of the above-ground part. Other variables were not different among plants in the different treatments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lamiaceae; Mint; Leaf area; Hydropony; Dry matter.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300003

Bee community of a beach dune ecosystem on Maranhão Island, Brazil
Provedor de dados: 52
Autores: Albuquerque,Patricia Maia Correia de; Camargo,João Maria Franco de; Mendonça,José Ângelo Cordeiro.
The bee-plant community in a beach dune ecosystem in north-eastern of Brazil was studied concerning phenology and floral preference. The bees visited thirty-three species of 20 families of plants. The most visited species were Vernonia arenaria (Asteraceae), Chamaecrista hispidula (Caesalpiniaceae), Passiflora foetida (Passifloraceae) and Turnera melochioides (Turneraceae). Fifty-five percent of plants presented an annual or long flowering period (from 5 to 7 months). The largest number of species blooming was observed from March to August (dry season), corresponding to the period of greatest abundance and diversity of bees. Based on the range of floral sources used by the dominant bees, three guilds of bees were noted: bees with a restricted range of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoidea; Bee-plant interactions; Beach dune; North-eastern Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700012

Haematological Parameters of the Hybrid Surubim (Pseudoplatystoma reticulatum x P. corruscans) farmed in Brazil
Provedor de dados: 52
Autores: Jerônimo,Gabriela Tomas; Brum,Aline; Pádua,Santiago Benites de; Gonçalves,Eduardo Luiz Tavares; Capecci,Robson Soares; Ishikawa,Márcia Mayumi; Martins,Maurício Laterça.
This study evaluated the haematological parameters of the hybrid surubim (Pseudoplatystoma corruscans x P. reticulatum) farmed in intensive and semi intensive system. A total of 240 fish were examined, 120 from fish farm and 120 from a semi-intensive for comparison between the hot and cold season. The water quality was weekly measured to evaluate the influence of environmental conditions on the haematology. Fish from intensive system showed hematocrit and white blood cell count (WBC) higher in hot season while the total plasmatic protein, hemoglobin concentration, mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV) were higher in cold season. In fish from semi intensive system, the red blood cells count (RBC) and total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture system; Hematology; Seasonality; Siluriformes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200254

Spatial Variation of Bacteria in Surface Waters of Paranaguá and Antonina Bays, Paraná, Brazil
Provedor de dados: 52
Autores: Kolm,Hedda Elisabeth; Schoenenberger,Maria Fernanda; Piemonte,Mariana da Rocha; Souza,Patrícia S. de A.; Scühli,Guilherme Schnell e; Mucciatto,Mariele B.; Mazzuco,Roseli.
Spatial variability in the concentration of total bacteria, saprophytes and coliforms abundance was investigated in surface waters of Paranaguá and Antonina Bays. Six points along a profile from the entrance of Paranaguá Bay to the innermost part of Antonina Bay, were sampled on February 26, 1997. Temperature, salinity, dissolved oxygen, pH, particulate organic carbon, water transparency and seston were also measured. Determination of total bacterial abundance and biomass were made with the epifluorescent method. Saprophytic bacteria counts were conducted with ZoBell 2216E culture medium with fresh and 32‰ water and the coliform MPN was enumerated with the chromogenic substrate method. Principal component analysis of biotic and abiotic data showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Hydrographic variables; Estuary; Paranaguá and Antonina Bays.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100005

Can lychee reducing the adipose tissue mass in rats?
Provedor de dados: 52
Autores: Almeida,Martha Elisa Ferreira de; Ferreira,Juliana Thays; Augusto-Obara,Thalita Riquelme; Cruz,Richtier Gonçalves da; Arruda,Henrique Silvano; Santos,Virgínia Souza; Ramos,José Antônio de Souza Cruz; Botrel,Diego Alvarenga; Botrel,Regiane Victória de Barros Fernandes.
ABSTRACT Lychee fruit has been studied owing to the presence of several bioactive compounds that can contribute to weight loss in obese individuals. However, the anti-obese potential of the fruit has not been explored yet. The aim of this study was to investigate the effect of different parts of lychee in reducing the adipose tissue mass of cafeteria diet-fed rats. Phenolic compounds and scavenging capacities were quantified. The food intake, apparent digestibility, weight of the body and liver, body mass, Lee Index, and the mass of epididymal and visceral adipose tissues were evaluated. The results were estimated by Tukey’s Test at 5% probability. A higher amount of phenolic compounds and scavenging capacity were observed in the peel of lychee as compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolics; Cafeteria diet; Obesity; Litchi chinensis Sonn.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100413

An Efficient Human Identification through MultiModal Biometric System
Provedor de dados: 52
Autores: Meena,K.; Malarvizhi,N.
ABSTRACT Human identification is essential for proper functioning of society. Human identification through multimodal biometrics is becoming an emerging trend, and one of the reasons is to improve recognition accuracy. Unimodal biometric systems are affected by various problemssuch as noisy sensor data,non-universality, lack of individuality, lack of invariant representation and susceptibility to circumvention.A unimodal system has limited accuracy. Hence, Multimodal biometric systems by combining more than one biometric feature in different levels are proposed in order to enhance the performance of the system. A supervisor module combines the different opinions or decisions delivered by each subsystem and then make a final decision. In this paper, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multimodal Biometrics Face Finger print Iris Contourlet transform; Classification Support Vector Machine NN classifier.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300403

Polyhydroxybutyrate production using agro-industrial residue as substrate by Bacillus sphaericus NCIM 5149
Provedor de dados: 52
Autores: Ramadas,Nisha V.; Singh,Sudheer Kumar; Soccol,Carlos Ricardo; Pandey,Ashok.
The aim of this work was to study the production of polyhydroxybutyrate (PHB) using agro- industrial residues as the carbon source. Seven substrates, viz., wheat bran, potato starch, sesame oil cake, groundnut oil cake, cassava powder, jackfruit seed powder and corn flour were hydrolyzed using commercial enzymes and the hydrolyzates assessed for selecting the best substrate for PHB production. Jackfruit seed powder gave the maximum production of PHB under submerged fermentation using Bacillus sphaericus (19%) at the initial pH of 7.5.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus sphaericus; Polyhydroxybutyrate; Jackfruit seed powder.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100003

A new biosurfactant produced by Candida glabrata UCP 1002: characteristics of stability and application in oil recovery
Provedor de dados: 52
Autores: Luna,Juliana Moura de; Sarubbo,Leonie; Campos-Takaki,Galba Maria de.
The production of a new biosurfactant by Candida glabrata UCP1002 was studied to evaluate the influence of the concentration of the cotton seed oil, glucose and yeast extract. The dynamics of the growth and surfactant production were showed for all the cultivation conditions studied. The best emulsification of the n-hexadecane, quantified by the emulsifying index was observed in the medium containing 7.5% cotton seed oil, 5% glucose and 0.3% yeast extract. The isolated biosurfactant showed a CMC of 2.5% and the surface tension at that point showed to be 31mN/m. The potential application of the biosurfactant in oil recovery from the sand, in acid and alkaline environments and over exposure to high salinity and different temperatures was demonstrated by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactant; Candida glabrata; Surface tension; Stability; Cotton seed oil; Oil removal.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400001

Evaluation of fractions and 5,7-dihydroxy-4',6-dimethoxy-flavone fromClerodendrum phlomidis Linn. F. against Helicoverpa armigera Hub.
Provedor de dados: 52
Autores: Duraipandiyan,Veeramuthu; Muthu,Chelliah; Baskar,Kathirvelu; Al-Dhabi,Naif Abdullah; Ignacimuthu,Savarimuthu.
Twelve fractions from chloroform extract of Clerodendrum phlomidis and 5,7-dihydroxy- 4',6-dimethoxy-flavone (pectolinaringenin) were evaluated against Helicoverpa armigera. Maximum antifeedant (89.41%), larvicidal (83.77%) and ovicidal (69.25%) activities were observed in fraction 5. The least LC50 value for antifeedant (178.09 ppm) and larvicidal (198.23 ppm) were observed in fraction 5. No adult emergence was recorded in fractions 4-6 at 1000 ppm. The oviposition deterrent activity was 100% in fraction 5 at all the concentrations. Pectolinaringenin recorded maximum antifeedant (74.68%) and larvicidal (81.11%) activities at 100 ppm; it completely prevented the adult emergence of H. armigera at 100 ppm. Maximum ovicidal activity at 100 ppm concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adult emergence; Antifeedant; Larvicidal; Ovicidal; Oviposition deterrent; Pectolinaringenin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200216

Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae)
Provedor de dados: 52
Autores: Monteiro,Juliana Castro; Matta,Sérgio Luis Pinto da; Predes,Fabrícia de Souza; Oliveira,Tânia Toledo de.
Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Congonha-de-bugre; Rubiaceae; Medicinal plants.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200018

Sightings of Pontoporia blainvillei (Gervais &amp;amp; D' Orbigny, 1844) and Sotalia fluviatilis (Gervais, 1853) (Cetacea) in South-eastern Brazil
Provedor de dados: 52
Autores: Di Beneditto,Ana Paula M.; Ramos,Renata Maria A.; Lima,Neuza Rejane W.
Pontoporia blainvillei (Gervais &amp;amp; D' Orbigny, 1844) and Sotalia fluviatilis (Gervais, 1853) have typically coastal habits and are sympatric in South-eastern Brazil. The purpose of this work was to record sightings and describe aspects concerning the behaviour of both species in this region, between 1993 and 1998. The sightings were accomplished by cruises and from a fixed point. Information provided by fishermen were also considered. In general, P. blainvillei was observed in all seasons of the year, up to five nautical miles away from the coast, up to 15 m deep and the groups comprised up to five specimens. The most part of the groups of S. fluviatilis, were observed in autumm and winter times and comprised up to 10 specimens. In about half of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pontoporia blainvillei; Sotalia fluviatilis; Sightings; South-eastern Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300011

Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene
Provedor de dados: 52
Autores: Ahmed,Abdul Bakrudeen Ali; Rao,Adhikarla Suryanarayana; Rao,Mandali Venkateswara; Taha,Rosna Mat.
The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid (100 mg/L) on 6th subcultures. Further development stages such as torpedo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryogenesis (SEs); Picloram; Ascorbic acid; Coconut milk; Plant growth regulators; Murashige and Skoog medium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100002

Alterations on growth and gill morphology of Danio rerio (Pisces, Ciprinidae) exposed to the toxic sediments
Provedor de dados: 52
Autores: Fracácio,Renata; Verani,Nelsy Fenerich; Espíndola,Evaldo Luiz Gaeta; Rocha,Odete; Rigolin-Sá,Odila; Andrade,Cássio Arilson.
The objective of this work was to assess the toxicity of sediment samples from six cascade reservoirs in Tietê river system, in São Paulo State, Brazil, through chronic-partial toxicity bioassays with Danio rerio larvae as test-organisms. Histology of gills and biometric measurements were used to determine the existence of toxicity problems. The alterations on gill morphology here detected were considered of first stage (hyperplasia, lamellar junction and excess of mucous cells) in the first reservoirs and very slight in the last ones. The biometric analysis pointed to inadequate conditions for the growth of the test-organisms when exposed to the sediment of the rivers and upstream reservoirs and also indicated an improvement of environmental conditions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gill morphology; Sediment toxicity; Danio rerio; Tietê reservoirs; Pollution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400023

Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus
Provedor de dados: 52
Autores: Cazetta,Marcia Luciana; Monti,Rubens; Contiero,Jonas.
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kluyveromyces marxianus var. bulgaricus; Sucrose; Inulinase; Biomass; Batch fermentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300027

Evaluation of toxic effects with transition metal ions, EDTA, SBTI and acrylic polymers on Aedes aegypti (L., 1762) (Diptera: Culicidae) and Artemia salina (artemidae)
Provedor de dados: 52
Autores: Arruda,Eduardo José de; Rossi,Ana Paula Leão; Porto,Karla Rejane de Andrade; Oliveira,Lincoln Carlos Silva de; Arakaki,Andrea Haruko; Scheidt,Gessiel Newton; Soccol,Carlos Ricardo.
This work aimed to evaluate the toxicity of some insecticides compounds on Aedes aegypti and Artemia salina larvae. Bioassays were carried out to evaluate the toxic effect after of 24 and 72 h using the compounds or associations. The LC10, LC50 and LC90 values were obtained and utilized for toxicity comparations. For Ae. aegypti, LC50 were 32.65 mg L-1 in 24 h for Na2[EDTA-Cu(II)] and total mortality in 72 h for SAP-Na2[EDTA-Cu(II)].
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect control; Aedes aegypti; Artemia salina.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200012

Antidepressant effects of standardized extract of Commiphora mukul Engl. in olfactory bulbectomized rats
Provedor de dados: 52
Autores: Kalshetti,Padmaja Bhimashankar; Alluri,Ramesh; Thakurdesai,Prasad Arvind.
The objective of this work was to evaluate the effects of standardized hydroalcholic extract of Commiphora mukul (HECM) in animal model of chronic stress medicated depression, namely olfactory bulbectomy (OBX) model in rats. Effects of 14-day (subacute) oral pretreatment of HECM (50, 100 and 200 mg/kg) were evaluated on depression and stress related parameters on OBX rats. Separate groups for sham control, OBX control and positive controls namely imipramine (20 mg/kg), fluoxetine (30 mg/kg) and desipramine (15 mg/kg) were also maintained. Behavioral and physiological parameters in open field and elevated plus maze were recorded. HECM showed dose-dependent reversal of OBX-induced physiological effects such as reduction of body weight, body temperature,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidepressant Activity; Commiphora MukulEngl; Guggul; Olfactory Bulbectomy Model.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100041

Genetic divergence in sweet cassava cultivars using morphological agronomic traits and RAPD molecular markers
Provedor de dados: 52
Autores: Rimoldi,Fabrício; Vidigal Filho,Pedro Soares; Kvitschal,Marcus Vinícius; Gonçalves-Vidigal,Maria Celeste; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Costa,Tiago Ribeiro da.
In this work the genetic divergence among 14 sweet cassava cultivars was estimated by their morphological agronomic traits and RAPD molecular markers. The Tocher cluster analysis and the Nearest Neighbor Method were applied. The most dissimilar cultivars were Pão and Guaíra, Fécula Branca and Pão, and Pão and Caipira, while the most similar cultivar were the Fécula Branca and Branca 1, Branca 3 and Branca 1, and Guaíra and Branca 1. The Jaccard's coefficient showed that the most similar cultivars were Guaíra and Quarenta Quilos, while the most dissimilar were Branca 3 and Amarela da Rama Cinza. The divergence analysis indicated that promising crosses could be made between the Branca 3 cultivar and the Pão, Amarela 1, Fécula Branca and Amarela 2 cultivars...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Manihot esculenta; Multivariate analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600025

Genetic parameter estimates in yellow passion fruit based on design I
Provedor de dados: 52
Autores: Gonçalves,Gustavo Menezes; Viana,Alexandre Pio; Pereira,Messias Gonzaga; Bezerra Neto,Francisco Valdevino; Amaral Júnior,Antônio Teixeira do; Pereira,Telma Nair Santana; Gonçalves,Tiago José Menezes.
The aim of this work was to study the genetic parameters, to obtaine the progenies by crossings among the yellow passion fruits cultivars (Passiflora edulis f. flavicarpa), following the Design I. The results of the variance analysis demonstrated large variability to be exploited in the population. The trait number of fruit per plant presented the largest additive genetic variation coefficient, demonstrating that larger gains could be obtained for the trait. In terms of genetic action, number of fruits per plant, skin thickness and number of days by flowering have demonstrated to be characterized by the overdominance. Other important traits seem to have presented larger addictive effect than the dominance, as weight, length and width of fruits. The highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic gain; Genetic components; Nested design; Passiflora.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300002

Maize (Zea Mays L) landraces from the southern region of Brazil: contamination by Fusarium sp, zearalenone, physical and mechanical characteristics of the kernels
Provedor de dados: 52
Autores: Oliveira,Tatiana Roselena de; Jaccoud-Filho,David de Souza; Henneberg,Luciana; Michel,Milton Domingos; Demiate,Ivo Mottin; Pinto,Alessandra Teixeira Barbosa; Machinski Junior,Miguel; Barana,Ana Cláudia.
This work had as objectives the study of the physical and mechanical characteristics of maize kernels in relation to the contamination by Fusarium sp and by zearalenone in twenty landraces of maize from the southern region of Brazil. From the analyzed samples, 60 % has been considered to have a hard endosperm type and 40 % an intermediary one. A correlation between the physical and mechanical variables was observed as an indication that the higher is the proportion of hard endosperm, more dense will be the kernel and more force for its rupture will be necessary. The level of contamination by Fusarium sp was between 5.5 and 24.75% among the analyzed grains, correlating positively with the flotation index, indicating that the landraces of maize with a softer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungi; Texture of endosperm; Corn landraces; Mycotoxins.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700002

Abiotic factors influencing tropical dry forests regeneration
Provedor de dados: 52
Autores: Ceccon,Eliane; Huante,Pilar; Rincón,Emanuel.
Tropical dry forests represent nearly half the tropical forests in the world and are the ecosystems registering the greatest deterioration from the anthropogenic exploitation of the land. This paper presents a review on the dynamics of tropical dry forests regeneration and the main abiotic factors influencing this regeneration, such as seasonal nature, soil fertility and humidity, and natural and anthropic disturbances. The main purpose is to clearly understand an important part of TDF succession dynamics.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical dry forests; Regeneration; Disturbances; Seasonal variability; Fertility; Moisture.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300016

Comparison of methods for phenotypic stability analysis of cassava (Manihot esculenta Crantz) genotypes for yield and storage root dry matter content
Provedor de dados: 52
Autores: Kvitschal,Marcus Vinícius; Vidigal Filho,Pedro Soares; Scapim,Carlos Alberto; Gonçalves-Vidigal,Maria Celeste; Sagrilo,Edvaldo; Pequeno,Manoel Genildo; Rimoldi,Fabrício.
The objective of this work was to compare different phenotypic stability methods by using yield and storage root dry matter content data of eight cassava genotypes, assessed in eight environments in northwest of Paraná State, Brazil. All the methodologies applied showed to be able to study the stability of cassava genotypes, but each with its peculiarities. The methodologies of Eskridge, Annicchiarico and Lin and Binns were the most appropriated on situation with smaller effect of G x E interaction. The AMMI analysis and the Toler and Burrows methodology were the most specific on detailing specific adaptations of cassava genotypes to favorable and unfavorable environments. It could be suggested to use simultaneous AMMI analysis and Toler and Burrows...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Correlation; Stability; Safety first indexes; Non-linear regression; AMMI analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100022

Assessment of the Toxicity of Sub-chronic Low and High Doses of the Bio-insecticide Spinosad on the Liver, Kidney and the Cerebellum in Male Albino Mice
Provedor de dados: 52
Autores: El-Naggar,Sabry A; Eltantawi,Hala; Ibrahim,Mahrous A; Alm-Eldeen,Abeer.
ABSTRACT Spinosad (SPD) is a highly selective insect control product. However, it was reported that SPD has toxicity toward other non-target organisms. This study was conducted to address the toxic effect of two sub-chronic low and high doses; 35 and 350 mg/kg SPD on some biochemical, histological and immunohistochemical parameters of the liver, kidney and cerebellum. Thirty-six male Swiss mice were divided into three groups of 12 mice each; first group (G1) served as a control, second group (G2) received a low sub-chronic dose of SPD that is equal to 35 mg/kg, and third group (G3) received a high sub-chronic dose of SPD that is equal to 350 mg/kg. The results showed that mice which were received 350 mg/kg SPD showed a significant decrease in the body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bio-insecticide; Spinosad; Toxicity; Sub-chronic; Biochemical; Histological; Immunohistochemical; Alterations.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100306

Population dynamics, gregarious behavior and oviposition preference of Neomegalotomus parvus (Westwood) (Hemiptera: Heteroptera: Alydidae)
Provedor de dados: 52
Autores: Ventura,Maurício Ursi; Panizzi,Antônio Ricardo.
Interactions between Neomegalotomus parvus (Westwood) (Coreoidea: Heteroptera: Alydidae) and host plants were investigated including population monitoring throughout the year, gregarious behavior in the field, and oviposition preference among host plants and one non-host plant in field cages. Cowpea, Vigna unguiculata (L.) Walp., soybean, Glycine max (L.) Mill. cv. BR 37, pigeon pea, Cajanus cajan (L.) Mill., pigeon pea cv. Anão, and lablab, Dolichos lablab L. were monitored in the field. N. parvus was found on host plants during 11 months. The shortest period of occurrence was observed on soybean and the longest on lablab. Insects were found mostly on mature pods. Contagious distribution (negative bionomial) was detected, characterizing the aggregation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemiptera; Alydidae; Host plants; Phenology; Oviposition.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100006

Characterization of Antimycins – Producing Streptomycete Strain VY46 Isolated from Slovak Soil
Provedor de dados: 52
Autores: Charousová,Ivana; Steinmetz,Heinrich; Medo,Juraj; Javoreková,Sona; Wink,Joachim.
ABSTRACT The strain no. VY46 was isolated from agricultural soil of Slovak republic and tested for potential antimicrobial activity against various human pathogens. On the basis of results, strain VY46 significantly inhibited growth of yeast Candida albicans and therefore was used for further characterization. In order to explore the potential bioactivities, extract of the fermented broth culture was prepared with organic solvent extraction method. The ethylacetate extract was subjected to HPLC fractionation against Candida albicans and followed by LC/MS analysis for potential production of anticandidal substances. The analysis resulted in the identification of two antimycins antibiotics, which may be responsible for important anticandidal activity of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomycetes; Anticandidal activity; Metabolite production; Antimycins antibiotics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100349

Impact of commercial fishing on Trindade Island and Martin Vaz Archipelago, Brazil: characteristics, conservation status of the species involved and prospects for preservation
Provedor de dados: 52
Autores: Pinheiro,Hudson Tercio; Martins,Agnaldo Silva; Gasparini,João Luiz.
Visual records, radio interviews and onboard observations of the fishing activities during a 58 days scientific expedition to Trindade Island and the Martin Vaz Archipelago were obtained from February to April 2007. The regular activities of four different fisheries were observed: pelagic longline, bottom line, trolling and handline. These fisheries caused mortality of at least seven species with some level threat, according to the International Union for Conservation of Nature list. The establishment of specific restriction norms for hook-and-line activities around the oceanic islands was seen as an alternative for the conservation of the ichthyofauna of the region. Satellite monitoring to track the fishery vessels could be established through the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocean fishery; Trindade Island; Martin Vaz Archipelago; Longline; Bottom line; Conservation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600018

Microsatellite analysis of the parental contribution of Piaractus mesopotamicus to the production of offspring in the semi-natural system of reproduction
Provedor de dados: 52
Autores: Povh,Jayme Aparecido; Ribeiro,Ricardo Pereira; Sirol,Rodolfo Nadez; Streit Jr.,Danilo Pedro; Moreira,Heden Luiz Marques; Siewerdt,Frank; Lopera-Barrero,Nelson Mauricio; Mangolin,Claudete Aparecida; Vargas,Lauro.
The objective of this study was to evaluate the genetic diversity and the parental contribution of Piaractus mesopotamicus in the production of offspring in the semi-natural system of reproduction. Twenty parental fishes (eleven males and nine females) and the total of 100 larvae were evaluated by microsatellite marker. The parents and offspring had thirty-one alleles and heterozygosity of 0.550 and 0.563, respectively. The females were fertilised by two up to six males while the males fertilised three up to five females. The contribution of the females and males to the offspring were 66.6 and 58%, respectively. Such results indicated no loss in the genetic variability in the offspring, and the parents had multiple paternity and reasonable contribution to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Stock enhancement programme; Reproductive management.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200018

Radiation-induced Changes in the Electrophoretic Profile of Serum Albumin
Provedor de dados: 52
Autores: Lima,Celso Vieira; Campos,Tarcisio Passos Ribeiro.
ABSTRACT Albumin protein profiles were investigated in electrophoresis system in relation to the whole body exposition to the radiation. Two groups of rats Wistar were set up as the control (CG) and the irradiated one (IG). The IG was exposed to Co-60 at a dose of 5 Gy. After a 72-hour exposition, 300 μL of blood was collected in the inferior vena cava, renal, jugular, hepatic, and pulmonary veins and the serum separated. The albumin protein was identified by vertical electrophoresis in acrylamide Commassi blue or silver stained. The calibration procedure was applied to albumin samples with well-known concentrations. The mathematical correlation was developed involving electrophoretic parameters of band intensities and sizes from gel representation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serum protein; Rats; Radiation; Albumin; Electrophoresis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100444

Imposex in endemic volutid from Northeast Brazil (Mollusca: Gastropoda)
Provedor de dados: 52
Autores: Castro,Ítalo Braga de; Meirelles,Carlos Augusto Oliveira de; Matthews-Cascon,Helena; Rocha-Barreira,Cristina de Almeida; Penchaszadeh,Pablo; Bigatti,Gregório.
Imposex is characterized by the development of masculine sexual organs in neogastropod females. Almost 120 mollusk species are known to present imposex when exposed to organic tin compounds as tributyltin (TBT) and triphenyltin (TPT). These compounds are used as biocide agents in antifouling paints to prevent the incrustations on boats. Five gastropod species are known to present imposex in Brazil: Stramonita haemastoma, Stramonita rustica, Leucozonia nassa, Cymathium parthenopeum and Olivancillaria vesica. This paper reports the first record of imposex observed in the endemic gastropod Voluta ebraea from Pacheco Beach, Northeast Brazil. Animals presenting imposex had regular female reproductive organs (capsule gland, oviduct and sperm-ingesting gland) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Imposex Voluta ebraea Endemic; Brazil Organotin Contamination.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500024

Radiolabelled cytokines for imaging chronic inflammation
Provedor de dados: 52
Autores: Signore,Alberto; D'Alessandria,Calogero; Annovazzi,Alessio; Scopinaro,Francesco.
Diagnosis and particularly follow-up of chronic inflammatory disorders could be often difficult in clinical practice. Indeed, traditional radiological techniques reveal only structural tissue alterations and are not able to monitor functional changes occurring in tissues affected by chronic inflammation. The continuous advances in the knowledge of the pathophysioloy of chronic disorders, combined with the progress of radiochemistry, led to the development of new specific radiolabelled agents for the imaging of chronic diseases. In this scenario, cytokines, due to their pivotal role in such diseases, represent good candidates as radiopharmaceuticals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiolabelled cytokines; Chronic inflammation; Imaging.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500004

Antimicrobial activity from endophytic fungi Arthrinium state of Apiospora montagnei Sacc. and Papulaspora immersa
Provedor de dados: 52
Autores: Ramos,Henrique Pereira; Braun,Glaucia Hollander; Pupo,Mônica Tallarico; Said,Suraia.
Papulaspora immersa and Arthrinium state of Apiospora montagnei Sacc. were isolated from the roots of Smallanthus sonchifolius (yacón). The crude extracts from their cultures inhibited the growth of Staphylococcus aureus, Kocuria rhizophila, Pseudomonas aeruginosa and Escherichia coli. The more relevant results were observed in the ethyl acetate extract from P immersa against P aeruginosa (90 µg/mL) and ethyl acetate extract from Arthrinium state of A montagnei Sacc. against P aeruginosa (160 µg/mL). The two endophytic fungi isolated from yacón roots as well as their antimicrobial activity detected in the crude extracts cultures were being reported for the first time.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthrinium state of Apiospora montagnei; Papulaspora immersa; Smallanthus sonchifolius; Yacón.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300017

Response of Paenibacillus polymyxa to iron: alternations in cellular chemical composition and the production of fusaricidin type antimicrobial compounds
Provedor de dados: 52
Autores: Raza,Waseem; Hongsheng,Wu; Qirong,Shen.
In this work, growth, cellular chemical composition and production of fusaricidin type antimicrobial compounds by P. polymyxa SQR-21 were compared in tryptone broth supplemented with four concentrations of iron (25, 50, 100 and 200 µM). The data revealed that the growth of P. polymyxa SQR-21 was increased by 3-8% with the increase in concentration of ferric ion (Fe3+). The production of fusaricidin type compounds was increased by 33-49% only up to 50 µM Fe3+ and the highest level of Fe3+ was inhibitory. Increase in the liquid culture Fe3+concentration increased the intracellular protein (2%), intracellular carbohydrate (14%), extracellular protein (7%) and polysaccharide contents (18%) while the intracellular lipid contents were increased (11%) only up to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical composition; Fusaricidin; Iron; Paenibacillus polymyxa; RNA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500019

Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)
Provedor de dados: 52
Autores: Aliko,Valbona; Hajdaraj,Gerta; Caci,Atenisa; Faggio,Caterina.
ABSTRACTDestabilisation of blood cell lysosomes in Mediterranean green crabCarcinus aestuarii was investigated using Neutral Red Retention Assay (NRRA). Crabs collected in Narta Lagoon, Vlora (Albania) during May 2014 were exposed in the laboratory to sub-lethal, environmentally realistic concentrations of copper. Neutral Red Retention Time (NRRT) and glucose concentration in haemolymph of animals were measured. The mean NRRT showed a significant reduction for the animals of the treatment group compared to the control one (from 118.6 ± 28.4 to 36.4 ± 10.48 min, p&lt;0.05), indicating damage of lysosomal membrane. Haemolymph glucose concentration was significantly higher in the treatment group (from 37.8 ± 2.7 to 137.8.4 ± 16.2 mg/dL, p&lt;0.05) than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcinus aestuarii; Neutral red retention assay; Lysosomal membrane destabilization; Bioindicator; Copper.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500750

Characterization of foams obtained from cassava starch, cellulose fibres and dolomitic limestone by a thermopressing process
Provedor de dados: 52
Autores: Schmidt,Vivian Consuelo Reolon; Laurindo,João Borges.
Cassava starch, dolomitic limestone and eucalypt cellulose fibres were used to prepare foam trays that could be used to pack foodstuffs. The influence of the cellulose fibre concentration in the composite formulation was investigated using 5, 10, 15, 20, 30 and 40% of fibres. The results indicated that an increase in cellulose fibre concentration promoted a decrease in density and tensile strength of the foam samples. The tensile strength at break for foam trays containing 5% of cellulose fibres was 3.03MPa, whilst the commercial trays of expanded polystyrene used to pack foods in supermarkets presented a tensile strength of 1.49 MPa. The elongation at break of the foam trays obtained in this work varied slightly with increase in cellulose fibre...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradable trays; Cassava starch; Eucalypt cellulose fibres; Limestone; Guar gum; Thermoprocessing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100023

Esterase polymorphism marking cultivars of Manihot esculenta, Crantz
Provedor de dados: 52
Autores: Resende,Adriana Gazoli; Vidigal Filho,Pedro Soares; Machado,Maria de Fátima P. S.
Esterase isozymes were used to detected substrate-preference polymorphism in twenty cultivars of Manihot esculenta, and to show cultivar-specific variation of this species. A relatively complex extraction solution of proteins from leaves was needed to show a larger number of esterase isozymes. Similarity between cultivars from six groups ranged from 51 to 96%. The cultivars identified by the same name seemed to be biochemically different regarding esterase isozymes. Esterase isozyme electrophoretic patterns could, therefore, be used to discriminate the cultivars identified by the same name, and to monitor the vegetative propagation of cultivars maintained in the germplasm collection. In breeding strategies, isoesterase analysis could be used to avoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Isozymes; Esterases; Manihot esculenta; Cultivar-specific isoesterases.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300003

Assessment of bacterial translocation in obstructive jaundice using Tc-99m Escherichia coli
Provedor de dados: 52
Autores: Diniz,Simone Odília Fernandes; Barbosa,Alfredo José Afonso; Araújo,Ivana Durval; Nelson,David Lee; Machado,Leonardo Augusto da Silva; Bernardo Filho,Mário; Cardoso,Valbert Nascimento.
In obstructive jaundice, the passage of bacteria and endotoxins through the intestine wall to reach the systemic circulation is associated with septicemia, renal failure and pulmonary dysfunction. The aim of this work was to investigate bacterial translocation utilizing 99mTc-E.coli in an experimental model of obstructive jaundice. The levels of bilirubin in rats that were subjected to ligature of the bile duct were significantly increased when compared to control animals (p &lt; 0.001). The biodistribution results did not show any translocation of 99mTc-E.coli to the mesenteric lymphatic nodules, liver, spleen or lungs of the rats that suffered ligature of the bile duct (p &gt; 0.05). The evaluation of the intestinal permeability using "per os"...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial translocation; Technetium-99m E coli; Obstructive jaundice.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700006

Exchangeable basic cations and nitrogen distribution in soil as affected by crop residues and nitrogen
Provedor de dados: 52
Autores: Rosolem,Ciro Antonio.
In this work, a greenhouse experiment was conducted to study the effects of N fertilization and residues of pearl millet, black oats and oilseed radish on pH and Ca, Mg, K, NO3-, and NH4+ distribution within the profile of a Distroferric Red Latosol. The equivalent of 8 t ha-1 of plant residues were placed on soil surface. Lime was applied on the soil surface and nitrogen was applied over the straw at 0, 50, 100, and 150 mg kg-1, as ammonium nitrate. Corn was grown for 57 days. Calcium contents and pH in the soil profile were decreased by Pearl millet residue, while black oat and oilseed radish increased Ca contents and these effects are not related with Ca contents in residue tissue. However, the presence of plant residues increased nitrate, ammonium, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Magnesium; Nitrogen; Potassium; Soil bases; No till.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300003

Chitosan reduces infection by Guignardia citricarpa in postharvest 'Valencia' oranges
Provedor de dados: 52
Autores: Rappussi,Maria Cristina Canale; Pascholati,Sérgio Florentino; Benato,Eliane Aparecida; Cia,Patrícia.
Citrus fruits are affected by the black spot disease caused by Guignardia citricarpa. Resistance induction is an alternative control measure and chitosan exhibits potential as resistance inducer. The effect of chitosan on G. citricarpa was evaluated in vitro and in 'Valencia' oranges. Citrus fruit were immersed into different chitosan concentrations. Chitosan (2%), combined with or without thiabendazole and the citric acid was also investigated. All the chitosan concentrations inhibited G. citricarpa mycelial growth and affected morphologically the conidial germination and appressorium formation. Chitosan inhibited the development of new lesions in oranges at room condition or under refrigeration. Thiabendazole and citric acid did not reduce the formation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Black spot; Citrus fruit; Alternative control; Resistance induction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300001

Comparison of diversity indices applied to macrophyte incidence-based data
Provedor de dados: 52
Autores: Evangelista,Heloísa Beatriz Antoniazi; Thomaz,Sidinei Magela; Evangelista,Luiz Roberto.
In this work, a recently proposed diversity index based on Patil and Taillie parametric diversity measure (or Tsallis entropy), Sq*, was applied to samples (presence-absence data) of macrophytes from the Itaipu Reservoir, Brazil. This new index was the value of the family of indices Sq for a specific evenness of a sample. Results demonstrated that the Shannon index and species richness showed expressively high correlation with the Sq*; however, the evenness had low correlation coefficients with the index Sq*, indicating that Sq* was particularly sensitive to rarity and species richness. On the other hand, the weak correlations of this index with evenness demonstrated that it was less sensitive to species relative abundances.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tsallis entropy; Macrophytes; Reservoir; Shannon; Simpson.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200014

Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge
Provedor de dados: 52
Autores: Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003

Non-parametric fitting of nonlinear equations to experi- mental data without use of initial guessing: a basic computer program
Provedor de dados: 52
Autores: Papamichael,Emmanuel M.; Evmiridis,Nickolaos P.; Potosis,Constaninos.
In this work, we present a non-parametric method, and the appropriate computer program, for fitting nonlinear multiparametric equations to experimental data. Our method is followed by computation of confidence limits of the parameter estimates. Its performance has been tested on several multiparametric equations, common in the fields of Biochemistry and Biotechnology, and it is a multiparametric expansion of the concept proposed by others for equations having more than two parameters. Good parameter estimates were obtained without a previous knowledge of initial parameter guessing values, and the proposed computer program converges rapidly, in all cases examined within this work.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-parametric methods; Computer program; Fitting of nonlinear equations.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100001

The response of young and adult rats to the riboflavin supplementation
Provedor de dados: 52
Autores: França,Camille Feitoza; Vianna,Lucia Marques.
The aim of this article was to study the response of young and adult rats on the supplementation of diet with riboflavin. Twenty-four young and adult normotensives (Wistar) male rats, subdivided into two groups: treated (10mg riboflavin/Kg of body weight) and control (receiving vehicle) were daily evaluated for physical and behavioural aspects. Systolic blood pressure was determined twice a week and liver toxicity was investigated it the end of treatment. Data were evaluated using one-way ANOVA and p&lt;0.05 was significant. There were no changes on general health aspects of the treated rats; however, the supplementation provoked a significant (p&lt;0.05) systolic blood pressure reduction.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Riboflavin; Antioxidant; Wistar.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400015

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences
Provedor de dados: 52
Autores: Lopez,Ana Maria Queijeiro; Lucas,John Alexander.
Thirty six isolates of fungi obtained from anthracnose lesions of cashew and associated host plants in Brazil, were compared by their cultural, morphological and partial sequences of the 28S ribosomal DNA characters. They showed a high degree of cultural variability. The average mycelial growth rate on all tested media ranged from 10.2-13.3 mm/day between the isolates. Most of them produced perithecia (sterile and fertile) and some produced setae (sterile and fertile). All the isolates produced acervuli with predominantly cylindrical conidia (12.4-17.7 µmX 4.8-6.0 µm in width) with round ends, which became septate on germination, and produced unlobed or slightlylobed appressoria. Comparison of the D2 domain of the large subunit (LSU) rDNA sequences with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthracnose; Inflorescence blight; Colletotrichum; RDNA; Cashew; Anacardium occidentale.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400001

Growth characteristics modeling of Bifidobacterium bifidum using RSM and ANN
Provedor de dados: 52
Autores: Meena,Ganga S.; Gupta,Suneel; Majumdar,Gautam C.; Banerjee,Rintu.
The aim of this work was to optimize the biomass production by Bifidobacterium bifidum 255 using the response surface methodology (RSM) and artificial neural network (ANN) both coupled with GA. To develop the empirical model for the yield of probiotic bacteria, additional carbon and nitrogen content, inoculum size, age, temperature and pH were selected as the parameters. Models were developed using ¼ fractional factorial design (FFD) of the experiments with the selected parameters. The normalized percentage mean squared error obtained from the ANN and RSM models were 0.05 and 0.1%, respectively. Regression coefficient (R²) of the ANN model showed higher prediction accuracy compared to that of the RSM model. The empirical yield model (for both ANN and RSM)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotics; Response surface methodology (RSM); FFD; Artificial neural network (ANN); Genetic algorithms (GA).
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600023

A comparative study on fungal laccases immobilized on chitosan
Provedor de dados: 52
Autores: Araújo,José Hilton Bernardino de; Uemura,Vinicius Oliveira; Moraes,Flavio Faria de; Barbosa,Aneli de Melo; Zanin,Gisella Maria.
The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laccases; Immobilization; Chitosan.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001

Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
Provedor de dados: 52
Autores: Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate.
ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular identification; Real-time PCR; Tospovirus vector.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454

Lipase catalyzed ester synthesis for food processing industries
Provedor de dados: 52
Autores: Rajendran,Aravindan; Palanisamy,Anbumathi; Thangavelu,Viruthagiri.
Lipases are one of the most important industrial biocatalyst which catalyzes the hydrolysis of lipids. It can also reverse the reaction at minimum water activity. Because of this pliable nature, it is widely exploited to catalyze the diverse bioconversion reactions, such as hydrolysis, esterification, interesterification, alcoholysis, acidolysis and aminolysis. The property to synthesize the esters from the fatty acids and glycerol promotes its use in various ester synthesis. The esters synthesized by lipase finds applications in numerous fields such as biodiesel production, resolution of the recemic drugs, fat and lipid modification, flavour synthesis, synthesis of enantiopure pharmaceuticals and nutraceuticals. It plays a crucial role in the food...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipase; Food applications; Ester; Esterification; Synthesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100026

Ractopamine as a metabolic modifier feed additive for finishing pigs: a review
Provedor de dados: 52
Autores: Almeida,Vivian Vezzoni de; Nuñez,Amoracyr José Costa; Miyada,Valdomiro Shigueru.
The β-adrenergic agonist ractopamine is increasingly used in the swine industry due to higher consumer demand for leaner pork products. Redirecting nutrients to favor leanness rather than fat deposition, ractopamine improves growth and carcass traits of finishing pigs. However, the impact of this agonist on pork quality is not clearly defined. Understanding the biological effects of dietary ractopamine dose, treatment period, lysine levels, and the lysine to metabolizable energy ratio will help pork producers achieve improvements in animal performance, carcass leanness, and economic efficiency in swine production systems.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-adrenergic agonist; Carcass leanness; Growth; Nutrition; Swine.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300016

Comparison of apolipoprotein E polymorphism and other atherosclerotic risk factors in two portuguese populations: the influence of insularity
Provedor de dados: 52
Autores: Lopes,Paula Alexandra; Gaspar,Gisela; Napoleão,Patrícia; Santos,Maria Cristina; Pavão,Maria Leonor; Viegas-Crespo,Ana Maria.
The main objective of this study was to investigate the apolipoprotein (apo) E genotypes in Portuguese populations from mainland (Lisbon city) and from San Miguel Island, Azores' Archipelago (Ponta Delgada city) and to look for differences between these particular sites in apparently healthy subjects. Also, subjects with clinical diagnosis of atherosclerotic disease were investigated in San Miguel Island. In Lisbon, the genotypes distribution was: epsilon3/epsilon 3 &amp;gt; epsilon 3/epsilon 4 &amp;gt; epsilon 2/epsilon 3 &amp;gt; epsilon 4/epsilon 4 while that, for Ponta Delgada and regardless the health condition, was: epsilon 3/epsilon 3 &amp;gt; epsilon 2/epsilon 3 &amp;gt; epsilon 3/epsilon 4. Within Ponta Delgada control group, females and males had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E polymorphism; Cardiovascular risk factors; Atherosclerosis; Insularity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500008

Borrelia sp. in naturally infected Didelphis aurita (Wied, 1826) (marsupialia: didelphidae)
Provedor de dados: 52
Autores: Abel,Isis dos Santos; Almeida Junior,Denclair Escobar de; Fonseca,Adivaldo Henrique da; Soares,Cleber Oliveira; Ishikawa,Márcia Mayumi.
Fifty-six opossums (Didelphis aurita) were captured on the campus of Universidade Federal Rural do Rio de Janeiro, Seropédica county, Rio de Janeiro state, in order to investigate the occurrence of Borrelia sp among them in relation with the study of spirochaetemia and its ectoparasites. Blood tests were made through dark field and phase contrast microscopy, as well as the obtainment of blood smears. Smears were stained with Giemsa stain, which did not prove efficacy. There was no relation between results obtained through blood tests (13 opossum positive for Borreliasp.), and this technique (two positive animals). Parasitaemia studies of 37 animals kept in captivity as well as of several recaptures in which animals once negative proved to be positive days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia sp.; Didelphis aurita; Marsupial; Amblyomma cajennense; Ctenocephalides sp.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300010

Feeding habits of the spider crab Libinia spinosa H. Milne Edwards, 1834 (Decapoda, Brachyura) in Ubatuba bay, São Paulo, Brazil
Provedor de dados: 52
Autores: Barros,Samara de Paiva; Cobo,Valter José; Fransozo,Adilson.
The main goal of this study was the identification of the items of the diet of the L. spinosa, based on the stomach contents analysis. The crabs were obtained from Ubatuba region north-eastern shore of São Paulo State. In the laboratory, all the individuals were dissected, the stomach was retreated and fixed in 10% formaline. The alimentary items were identified under stereomicroscope and analysed by the method of Frequency of Occurrence. A total of 194 stomachs was analysed and nine alimentary items were obtained. Unindentified material was found in 98% of analysed stomach and poriferan were present in less then 1% of stomachs. These results pointed a diversified diet explored by this crab, as well as the employment of some different methods for food...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stomach contents; Alimentary itens; Spider crab.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200023

Competitive-IgY- Enzyme Linked Immuno Sorbent Assay (CIgY-ELISA) to detect the cytokinins in Gerbera jamesonii plantlets
Provedor de dados: 52
Autores: Sousa,Cleiton Mateus; Miranda,Ricardo Motta; Freire,Ronald Bastos.
A competitive hyper-immune yolk Immunoglobulin Y - Enzyme Linked Immune Sorbent Assay (CIgY-ELISA), was developed as an alternative method to detect zeatin and 2ip in plantlets of gerbera. The endogenous level of hormones in the plantlets in vitro of gerbera with one or six weeks after replication was determined with competitive IgY-ELISA set to detect between 1 and 100 pmoles of plant hormone for each 1.0 g tissue. The plantlets of six weeks presented sprouts and root, while the plantlets of one week presented only sprouts. The CIgY-ELISA was set with high independent variables values of sensitivity/specificity of 96/89 % for zeatin and 94/78 % for 2ip, with high values of reproducibility (up to 90 %) for both the cytokinins. Zeatin content varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Determination of cytokinins; CIgY-ELISA; Zeatin; 2ip; Micropropagated-Gerbera jamesonii.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400001

Influence of stationary and bioreactor cultivation on Lentinula edodes (berk) pegler lignocellulolitic activity
Provedor de dados: 52
Autores: Regina,Magali; Broetto,Fernando; Giovannozzi-Sermanni,Giovanni; Marabotini,Rosita; Peranni,Claudio.
This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25º C and in a bioreactor with four complete rotations daily at 25º C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shiitake; Enzymes; Agricultural residues.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200001

Comparative anatomy of the absorption roots of terrestrial and epiphytic orchids
Provedor de dados: 52
Autores: Moreira,Ana Sílvia Franco Pinheiro; Isaias,Rosy Mary dos Santos.
The present study compared roots of terrestrial and epiphytic Orchidaceae, analyzing the anatomical characteristics from an ecological point of view. The material was collected at three different sites in Minas Gerais / Brazil and was fixed in FAA. Transverse sections were obtained by freehand sections or from material previously embedded in Paraplast® or Historesin®. The prominent characteristics of the epiphytic group were: significant smaller perimeter, epidermis with 3 or more cell layers, U-thickened exodermal cell walls, O-thickened endodermal cell walls, and a low ratio between the caliber and the number of protoxylem arches. The terrestrial group presented simple or multiseriate epidermis, and exodermis and endodermis with typical Casparian strips....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orchids; Terrestrial; Epiphytic; Anatomy; Adaptations.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100011

Histological Observation and Expression Patterns of antimicrobial peptides during Fungal Infection in Musca domestica (Diptera: Muscidae) Larvae
Provedor de dados: 52
Autores: Jiangfan,Xiu; Tao,Wang; Yu,Wang; Jianwei,Wu; Guo,Guo; Yingchun,Zhang; Xiaoli,Shang.
ABSTRACT Housefly, Musca domestica, has a complicated immune system. However, its underlying operating mechanism remains elusive. Candida albicans is a major pathogen affecting humans by causing deep infection fungous disease, but it is non-symbiotic in houseflies. To investigate the C. albicans infection process in housefly, the changes in morphological and histological and expression patterns of antimicrobial peptide were monitored to indicate the insect's response to fungal infection. The results showed that scattered brown spots were comprising melanized encapsulation and encapsulated fungal cells were initially observed at the inner side of larvae's body wall 3 h post-infection (PI). Between 6 and 36 h PI, the whole body of larvae was densely covered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musca domestica; Larvae; Candida albicans; Infection; Innate immunity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100340

Ichthyoplankton distribution and abundance off southeastern and southern Brazil
Provedor de dados: 52
Autores: Freitas,Débora Martins de; Muelbert,José Henrique.
This study describes the distribution of fish eggs and larvae along the southeastern and southern Brazilian coast. Plankton samples were collected at 85 stations using a Bongo net, and water salinity and temperature were profiled with a CTD. Results showed that fish eggs and larvae, and zooplankton biovolume were distributed in coastal waters with mean temperature of 23&amp;#186C and salinity between 33 and 34. The largest egg abundance occurred along Iguape (24&amp;#186'S) with a partial overlap with zooplankton biovolume and fish larvae were most abundant near shore close to Santos (24&amp;#186S). These protected coastal waters presented a surface layer with lower salinity and higher temperatures, while the bottom layer had cooler water. Ichthyoplankton...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ichthyoplankton; Southern Brazilian Continental Shelf; Abundance; Distribution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400014

Contribuição ao Conhecimento da Incidência de Leptospiras em Murídeos, Caninos e Suínos no Paraná
Provedor de dados: 52
Autores: Enrietti,Marcos Augusto.
This research carried out in the city of Curitiba took us to the following conclusions: 1) In the main zone of Curitiba city the leptospirosis index among the rats captured reached 78 percent. This index represent the greatest incidence verified till the present moment in Brazil. 2) The ‘Rattus norwegicus’ was the chief species among those rats (96 percent). 3) According to the results presented, the culturing methods are preferable to the inoculating one. 4) The combination of these two simple methods is more advantageous in the leptospira research.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Weil disease; Rattus norwegicus.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500015

Frequency-dependence of mating success in Poeciliopsis monacha (Pisces, Cyprinodontiformes) reproductive complex, Sonora, Mexico
Provedor de dados: 52
Autores: Lima,Neuza Rejane Wille; Bizerril,Carlos Roberto S. F.
A diversity of all-female fishes of the genus Poeciliopsis coexists with their sexual ancestor species in streams of western Mexico. All-females are hybrids that depend on the sperm of paternal species to reproduce. Rare-female advantage is one of several hypotheses that attempt to explain how the diversity of all-female biotypes is maintained within the Poeciliopsis reproductive complexes. According to this hypothesis, the uncommon all-female biotype has a mating advantage over the common ones and has been maintained by a dynamic equilibrium process. In the P. monacha reproductive complex at Arroyo de los Platanos the density of two all-female biotypes (P. 2monacha-lucida I and II) varies across pools. The objective of this study was to analyse fecundity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: All-females; Mating success; Poeciliopsis monacha; Rare-female advantage.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200006

Microflora dynamics in earthworms casts in an artificial soil (biosynthesol) containing lactic acid oligomers
Provedor de dados: 52
Autores: Alauzet,Nathalie; Roussos,Sevastianos; Garreau,Henri; Vert,Michel.
Studies were performed to appreciate the presence of micro-organisms able to degrade OLA, in earthworms casts or in the surroundings. Worms were grown in biosynthesol, an artificial soil. The counting of bacteria and fungi in earthworms casts and in biosynthesol without earthworms suggested that earthworms ate some of the micro-organisms. The main filamentous fungi genera found were Aspergillus, Trichoderma, Fusarium and Penicillium. Previous results in the literature have shown that some species from the Aspergillus and Fusarium genera were able to degrade OLA and other aliphatic esters. It could be suggested that these two genera and some bacteria were responsible for the pre-degradation of OLA, and that earthworms might eat them.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Earthworm; Eisenia andrei; Biodegradation; Lactic acid polymers; Artificial soil; Natural microflora.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200001

The Effect of PM 10 on Ischemia- Reperfusion Induced Arrhythmias in Rats
Provedor de dados: 52
Autores: Radmanesh,Esmat; Dianat,Mahin; Badavi,Mohammad; Goudarzi,Gholamreza; Mard,Seyyed Ali.
ABSTRACT Epidemiological studies show that particulate matter (PM) is the principal instigator of some adverse clinical symptoms involving cardiovascular diseases. PM exposure can increase experimental infarct size and potentiate myocardial ischemia and arrhythmias in experimental MI models such as ischemia-reperfusion (I/R) injury.The present study was aimed to evaluate the effects of particulate matter (PM10) on ischemia- reperfusion induced arrhythmias with emphasis on the protective role of VA as an antioxidant on them. Male Wistar rats were divided into 8 groups (n=10): Control, VAc, Sham, VA, PM1 (0.5 mg/kg), PM2 (2.5 mg/kg), PM3 group (5 mg/kg), PM3 + VA group. Within 48 hours, PM10 was instilled into trachea in two stages. Then the hearts were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Particullate Matter Ischemia- reperfusion arrhythmias; Vanillic Acid Rat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100339

Recovery of phytase produced by solid-state fermentation on citrus peel
Provedor de dados: 52
Autores: Rodríguez-Fernández,Daniel Ernesto; Rodríguez-León,José Angel; Carvalho,Júlio Cesar de; Thomaz-Soccol,Vanete; Parada,José Luis; Soccol,Carlos Ricardo.
The extraction of phytase produced by solid-state fermentation of citrus peel was studied employing a multistage leaching process. It was observed that the extracts containing EDTA retained over 90% of phytase activity at room temperature after 24 h after the leaching. A fractional design 2² (with 4 replicates at the central point) was carried out for testing the pH and agitation as process independent factors. Only the interaction between the pH and agitation showed a significant influence. These factors were optimized with a central composite design. Agitation at 300 rpm and pH at 5.0 were the best conditions to extract the enzyme from solid matrix. The modeling of the process indicated that diffusivity of the enzyme in the solvent was the controlling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaching; Recovery multistage; Phytase; Solid state fermentation; Kinetic models.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600026

Survey of natural radioactivity levels in Ilex paraguariensis (St. Hil.) by gamma-ray spectrometry
Provedor de dados: 52
Autores: Scheibel,Viviane; Appoloni,Carlos Roberto.
The mate tea (Ilex paraguariensis - St. Hil.) is a beverage broadly cultivated and consumed in Brazil. The radioactive traces present in three trademarks of toasted mate tea produced at the south of Brazil were analyzed. Measurements were carried out by gamma-ray spectrometry. The average value for the 40K activity measured for marks A, B and C was 1216 ± 8, 1047 ± 14 and 666 ± 13 Bq.kg-1, respectively. The 137Cs activity was lower than the limit of detection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma-ray spectrometry Marinelli beaker mate tea; 40K.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500019

Generation of Photovoltaic Solar Energy. Evaluation of the Demand Curve with the Insert of Grid-connected Photovoltaic Power System at CINDACTA II, Curitiba-PR, Brazil.
Provedor de dados: 52
Autores: Ribeiro,Rosiane Bonatti; Silva,Ramon Bulhões e; Frigotto,Rodrigo Luiz; Urbanetz Junior,Jair.
ABSTRACT Unlike some countries, Brazil has a predominantly hydraulic energy matrix, a clean and renewable source. But, in recent years, both the non-renewal of the normal volumes of water in the reservoirs and the lack of consciousness in the consumption of water and energy have placed the country in a critical state of energy supply leading to many intensive policies to reduce its consumption. In contrast, energy from Photovoltaic (PV) on-Grid Systems has grown dramatically in recent years. In this sense, this study presents an analysis of the contribution of the energy generated by a PV on-Grid Systems to be installed in CINDACTA II, in order to make re-contracting a lower demand possible and also reduce electric energy consumption and its cost.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic System; Demand; Renewable Energy; Save Energy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200232

cDNA cloning, Phylogenic Analysis and Gene Expression Pattern of Phenylalanine ammonia-lyase in Sugarcane (Saccharum officinarum L.)
Provedor de dados: 52
Autores: Hashemitabar,Mahmoud; Kolahi,Maryam; Tabandeh,Mohammad Reza; Jonoubi,Parisa; Majd,Ahmad.
The aim of the present study was to clone and characterize a full length cDNA of sugarcane (Saccharum officinarum) phenylalanine ammonia-lyase (SoPAL). Differential tissue expression pattern of the SoPAL transcript and its enzyme activity was also analyzed during the tillering stage of growth. The full-length of SoPAL cDNA was 2118 bp long and contained a protein with 706 amino acids, determined by encoding technique. The amino acid sequence and phylogenic analysis of the cloned SoPAL showed high similarity to PAL from other monocotyledonous such as sorghum (96%), maize (93%) and Bamboos (87.12%). The highest levels of SoPAL transcript were observed in the root and stem, while its minimal gene expression levels were in the leaves and sheath, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenylalanine ammonia-lyase; Saccharum officinarumL; Cloning; Tissue expression.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400456

Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants
Provedor de dados: 52
Autores: Al-Rasheed,Nouf Mohamed; Fadda,Laila Mohamed; Al-Rasheed,Nawal Mohamed; Ali,Hanaa Mahmoud; Yacoub,Hazar Ibrahim.
The objective of this study is to examine whether silymarin alone or in combination with chlorogenic acid and/ or melatonin plays a modulatory role against apoptotic damage in rats liver induced by of CCl4. The present work revealed that CCl4 induced elevation of in Bax, Smad, TGF-β and NFkBhepatic mRNA expression, administration of silymarin alone down regulates these expressions. Treatment with chlorogenic acid and/ or melatonin along with silymarin produced best results in this concern. Bcl-2 expression was down regulated by CCl4 whereas concurrent treatment of chlorogenic acid and/ or melatonin along with silymarin increased this expression. On conclusion, the use of chlorogenic acid and/ or melatonin potentiates the anti-apoptotic action of silymarin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCl4; Silymarin; Chlorogenic acid; Melatonin; MRNA expression.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100408

Evaluation of sequential aerated treatment of wastewater from hardboard mill
Provedor de dados: 52
Autores: Videla,S.; Isaacs,C.; Diez,M. Cristina.
Wastewater from a hardboard mill characterized by a high organic content (15-30 g/L COD) was studied in a bench scale sequential aerated system in order to define a start up strategy. Inlet COD concentration varied from 0.5 to 25 g/L and the hydraulic retention time was maintained at 5 days. The sequential system proposed could reduce BOD, COD, TSS and phenol over 90% except when the inlet COD concentration was lower than 25 g/L.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fiber board wastewater; Hardboard; Sequential aerobic treatment; Start up strategy.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300002

Effects of temperature and salinity on the survival rates of coxicerberus ramosae (Albuquerque, 1978), an interstitial isopod of a Sandy Beach on the coast of Brazil
Provedor de dados: 52
Autores: Albuquerque,Elaine Figueiredo; Meurer,Bruno; Netto,Godofredo da Camara Genofre.
The tolerance to the combined effects of temperature and salinity was investigated in the interstitial isopod Coxicerberus ramosae (Albuquerque, 1978), a species of intertidal zone of sandy beaches in Rio de Janeiro, Brazil. The animals were collected on Praia Vermelha Beach. The experiments lasted 24 h and nine salinities and seven temperatures were used for a total of 63 combinations. Thirty animals were tested in each combination. The species showed high survival in most of the combinations. The temperature of 35 ºC was lethal and at 5 ºC, the animals tolerated only a narrow range of salinities. The statistical analyses showed that the effects of temperature and salinity were significant on the survival, which confirmed the euryhalinity and eurythermy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interstitial; Isopod; Resistance; Temperature; Salinity; Coxicerberus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500015

Interactions between an arbuscular mycorrhizal fungus (Scutellospora heterogama) and the root-knot nematode (Meloidogyne incognita) on sweet passion fruit (Passiflora alata)
Provedor de dados: 52
Autores: Anjos,Érika Cristina Teixeira dos; Cavalcante,Uided Maaze Tiburcio; Gonçalves,Danielle Maria Correia; Pedrosa,Elvira Maria Regis; Santos,Venézio Felipe dos; Maia,Leonor Costa.
The effects of inoculation of sweet passion fruit plants with the arbuscular mycorrhizal (AM) fungus Scutellospora heterogama on the symptoms produced by Meloidogyne incognita race 1 and its reproduction were evaluated in two greenhouse experiments. In the 1st, the M. incognita (5000 eggs/plant) and S. heterogama (200 spores/plant) inoculations were simultaneous; in the 2nd, the nematodes were inoculated 120 days after the fungal inoculation. In both the experiments, 220 days after AM fungal inoculation, plant growth was stimulated by the fungus. In disinfested soil, control seedlings (without S. heterogama) were intolerant to parasitism of M. incognita, while the growth of mycorrhized seedlings was not affected. Sporulation of S. heterogama was negatively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AMF; Biological control; Mycorrhizal symbiosis; Nematode reproduction; Passifloraceae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400008

Design of signal peptide bombyxin and its effect on secretory expression efficiency and levels of Helicobacter pylori urease subunit B in silkworm cells and larvae
Provedor de dados: 52
Autores: Zhang,Xiao-lin; Jiang,An-min; Ma,Zhong-you; Xiong,You-yi; Dou,Jin-feng; Zhou,Guo-liang; Qin,Mei-song; Wang,Jian-fei.
This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori.The signal peptide bombyxin from B. mori was used to promote secretory expression to improve expression levels and was designed and integrated into the UreB gene to generate the Bacmid/BmNPV/(signal peptide)-UreB baculovirus expression system. To determine whether the bombyxin signal peptide resulted in secretory expression of recombinant UreB (rUreB) and to determine the secretory efficiency, we tested the secretory expression level of rUreB in Bm5 cells using ELISA. To further investigate whether secretory expression affected cell viability, cells were evaluated using 0.4% trypan blue staining, and Bacmid/BmNPV/UreB without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus expression system; Bombyx mori; Signal peptide; Helicobacter pylori urease subunit B; Secretory expression.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300319

Ecofriendly Synthesis of Silver Nanoparticles Using Potato Steroidal Alkaloids and Their Activity Against Phytopathogenic Fungi
Provedor de dados: 52
Autores: Almadiy,Abdulrhman A.; Nenaah,Gomah E.
ABSTRACT In order to reduce the excessive reliance on the toxic chemical fungicides, the present study aimed to isolate the total potato glycoalkaloids (TPAs), and the two steroidal alkaloids α-chaconine and α-solanine from potatoes, Solanum tuberosum L. Their structures were characterized using physical and spectroscopic methods including (UV, IR, 1H, 13C--NMR, 2D 1H-1H COSY, HMBC and NOESY). Silver nanoparticles (AgNPs) were prepared from potato alkaloids through a green synthesis approach. Potato alkaloids and their nanoparticles inhibited mycelial growth of the phytopathogenic fungi Alternaria alternate, Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum f. sp. lycopersici with low minimal inhibitory and minimal fungicidal concentrations. R....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycoalkaloids; Α-chaconine; Α-solanine nanoparticles; Fungicidal activity; Phytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100203

Nutrient composition of mature and litter leaves and nutrient mobilization in leaves of tree species from secondary rainforests in the South of Brazil
Provedor de dados: 52
Autores: Dickow,Kauana Melissa Cunha; Marques,Renato; Pinto,Carolina Benghi.
The aim of this work was to analyze the concentration of N, P, K, Ca and Mg in mature and litter leaves and nutrient mobilization in leaves of different tree species from secondary forests of the Atlantic Forest. At the study site, located in Floresta do Palmito (Paranaguá, PR), three different forest typologies namely Initial, Intermediary and Advanced were selected. The predominant sequence of nutrients in litter leaves was: N &gt; Ca &gt; Mg &gt; K &gt; P. This sequence was very similar to that found for mature leaves, the difference being only the change in order between Mg and K. Nutrients mobilized in leaves were higher in the typology Advanced compared to the typology Initial, mainly N, P and K. The majority of the species studied did not mobilize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic forest; Biogeochemical cycling; Forest nutrition; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500006

External morphology of the adult of Heraclides anchisiades capys (Hübner, [1809]) (Lepidoptera - Papilionidae) I: head, cephalic appendages and cervical region
Provedor de dados: 52
Autores: Leite,Luis Anderson Ribeiro; Casagrande,Mirna Martins; Mielke,Olaf Hermann Hendrik.
The purpose of this study was to highlight the morphological components of the head, its appendages and the cervical region of the adults of Heraclides anchisiades capys (Hübner, [1809]), looking for a comparative focus with the other Papilionoidea and Hesperioidea. The most relevant morphological characters were: presence of transclipeal band, laterofacial suture in ventral view near the half of the ocular margin of convex aspect, presence of rudimental maxillary palp; union of the cervical sclerites ventromedially and ventral sclerite situated before the merger of the lateral sclerites.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Neotropical; Papilioninae; Papilionoidea.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500016

Soil flood tolerance of seven genotypes of Panicum maximum Jacq.
Provedor de dados: 52
Autores: Silva,Adriane Schio; Laura,Valdemir Antônio; Jank,Liana.
The soil flood tolerance of seven genotypes of Panicum maximum Jacq. (PM11, PM34, PM40 and PM45, and the commercial cultivars Massai, Mombaça and Tanzânia) was evaluated in plants subjected to two conditions: flooded and not flooded, during a period of 14 days. Flooding significantly decreased the total and above ground biomass of PM40 and PM45. For cultivar Tanzânia, flooding decreased these two variables and also root biomass. The root, total and above ground relative growth rates were significantly reduced by flooding in cultivar Tanzânia, while in PM45 only the above ground relative growth rate was reduced. Cultivar Tanzânia showed significant differences for all variables analyzed, thus was not flood tolerant, as well as PM40 and PM45. It could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anoxia; Flooding; Forage grass.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600004

Isolation, Characterization, Antioxidant, Antimicrobial and Cytotoxic Effect of Marine Actinomycete, Streptomyces Carpaticus MK-01, against Fish Pathogens
Provedor de dados: 52
Autores: Subramanian,Dharaneedharan; Kim,Min-Sun; Kim,Dong-Hwi; Heo,Moon-Soo.
ABSTRACT Present study aim to evaluate the antimicrobial, antioxidant and cytotoxic potential of crude extract of Marine Streptomyces carpaticus MK-01 isolated from seawater collected from Daejeong-cost of Jeju Island. About 24 actinomycetes strains were isolated and subjected to morphological and molecular analysis that confirmed the isolate as S. carpaticus MK-01. Crude ethyl acetate extract of MK-01 strain showed extensive antibacterial activity against Gram-positive fish pathogenic bacteria namely Streptococcus iniae and S. parauberis with a maximum zone of inhibition (0.92±0.03mm) was recorded against S. parauberis at the minimum extract concentration (3.12µg/ml). The MK-01 ethyl acetate extract shows dose dependant significant increase in antioxidant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinomycete; Streptomyces carpaticus MK-01; Antioxidant; Cytotoxicity; Jeju Island.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100437

In vivo and in vitro protein digestibility of formulated feeds for Artemesia longinaris (Crustacea, Penaeidae)
Provedor de dados: 52
Autores: Fernández Gimenez,Analía Verónica; Díaz,Ana Cristina; Velurtas,Susana María; Fenucci,Jorge Lino.
This study was undertaken to determine the in vivo crude protein apparent digestibility in the prawn Artemesia longinaris, using feeds with 0.25% of chromic oxide and animal (fish meal, meat and bone meal and squid protein concentrate) and plant (soybean meal) ingredients. Three replicate groups of prawn were fed and the feces were collected. The rate of protein hydrolysis was measured in vitro using midgut gland enzyme extract from the prawns fed the respective feeds and was compared with those found with enzyme extract of wild prawn. The in vivo apparent digestibility coefficients showed significant differences among the feeds (P&lt;0.05). Fish meal feed presented the highest digestibility (92%); intermediate digestibility (83%) was found for meat and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prawn; Nutrition; Protein sources; Enzyme assays.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600009

Biological Response of Tradescantia Stamen-hairs to High Levels of Natural Radiation in the Poços de Caldas Plateau
Provedor de dados: 52
Autores: Gomes,Heliana de Azevedo; Nouailhetas,Yannick; Silva,Nivaldo Carlos da; Mezrahi,Arnaldo; Almeida,Carlos E. Bonacossa de; Rodrigues,Geraldo Stachetti.
The objective of the present study was to apply a highly sensitive botanical test of mutagenicity (the Tradescantia stamen-hair mutation bioassay) to assess in situ the biological responses induced by naturally occurring radiation in the Poços de Caldas Plateau. The mutagenesis was evaluated in environments presenting gamma radiation exposure rates ranging from 1.5 muR.min-1 up to 100.0 muR.min-1. The results consistently showed only borderline increases in mutation frequencies in plants exposed to areas with high radiation background, as compared to non-exposed plants. It was concluded that the levels of natural radiation prevalent in the Poços de Caldas Plateau were not sufficient to induce significant increases in mutation rate, even in the extremely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutagenesis; Natural radiation; Bioassay; Tradescantia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300008

Evaluation of sugar-cane bagasse as bioadsorbent in the textile wastewater treatment contaminated with carcinogenic congo red dye
Provedor de dados: 52
Autores: Raymundo,Aline Sartório; Zanarotto,Romina; Belisário,Marciela; Pereira,Madson de Godoi; Ribeiro,Joselito Nardy; Ribeiro,Araceli Verónica Flores Nardy.
A methodology involving sugar cane bagasse bioadsorbent was developed in order to remove the carcinogenic congo red dye from aqueous medium. The results showed high efficiency with retention of 64 ± 6% in synthetic congo red solution and 94 ± 5% in effluent enriched with congo red, at 10.0 g of the bioadsorbent. The adsorption system provided a maximum adsorption capacity of 4.43 mg/g. Tests showed independence adsorption properties, when compared with the column flow rates. The treatment units could be operated with flexibility. From the results, it was possible to conclude that sugar cane bagasse could be an adequate bioadsorbent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugar cane bagasse; Congo red; Bioadsorption.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400023

Antibiofilm, Antioxidant, Antimutagenic Activities and Phenolic Compounds of Allium orientale BOISS.
Provedor de dados: 52
Autores: Ceylan,Ozgur; Alıc,Halime.
ABSTRACT This is the first study to investigate the antibiofilm, antioxidant and antimutagenic activities and phenolic compounds of Allium orientale. Antimicrobial activity of ethanolic extracts of A. orientale was determined by a broth microdilution method. Antibiofilm activity was evaluated by microplate biofilm assay. The antioxidant activity was determined using three complementary assays; namely, DPPH scavenging, β-carotene-linoleic acid, and total phenolic compounds assays. Phenolic compounds were evaluated by reverse-phase high-performance liquid chromatography. The antimutagenic effect of extracts was analyzed by the Ames test. In RP-HPLC analysis, (+)-catechin, apigenin and caffeic acid were identified as major phenolic compounds in the aerial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium orientale; Antibiofilm activity; Antimutagenic activity; Antioxidant activity; Phenolic compounds.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600935

Action of Essential Oils on Sprouting of Non-Dormant Potato Tubers
Provedor de dados: 52
Autores: Finger,Fernando Luiz; Santos,Mirelle Mayana de Sousa; Araujo,Fernanda Ferreira; Lima,Paula Cristina Carvalho; Costa,Lucas Cavalcante da; França,Christiane de Fatima Martins; Queiroz,Matheus da Costa.
ABSTRACT The breakdown of dormancy and early sprouting is one of the main causes of losses of stored potatoes. Sprouting control becomes important especially when tubers are submitted to long-term storage for further processing by the potato industry. The objective of this work is to evaluate the effect of organic sprout inhibitors, eugenol and menthol essential oils, on the suppression of growth on non-dormant ‘Asterix’ tubers stored at 8 °C. Administration of eugenol and menthol essential oils reduced the number and the length of sprouts during cold storage up to fifty days. Nevertheless, the essential oils stimulated the break of bud apical dominance, stimulating auxiliary buds growth. The effect of the oils was restricted to inducing necrosis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Eugenol; Menthol; Buds; Periderm.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100206

Contribuição ao Conhecimento dos Solos dos Campos Gerais no Estado do Paraná
Provedor de dados: 52
Autores: Bodziak Jr.,Carlos; Maack,Reinhard.
The present work is the first contribution for the knowledge of the Campos Gerais soils, the study of which was started by the Biological Institute and Technological Research, in Curitiba. In it there are discussed the soils of the Vila Velha region. Exposed the geological constitution of the landscape integrated by devonian beds in the Campos Gerais serie and the glacial deposits of the Itararé formation, the results of petrographic-sediment researches and the coefficients of selection of various sediments are given. A rapid exposition of the phytogeographic and climate conditions - according to Koeppen = 7 Cfb - is followed by the presentation of the results of the physical and chemical researches made in the soils of eight profiles, illustrated with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Devonian beds; Vila Velha; Campos Gerais; Soil profiles.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500008

Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells
Provedor de dados: 52
Autores: Rebello,Bernardo Machado; Moreno,Silvana Ramos Farias; Ribeiro,Camila Godinho; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Caldas,Luis Querino de Araújo; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Labeling; Blood; Passiflora edulis f. flavicarpa; Radiobiocomplexes; Technetium-99m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600019

Erratum: The Nano-Magnetic Dancing of Bacteria Hand-in-Hand with Oxygen
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100914

Physiological Changes in the Seeds of Jatropha curcas L. at Different Stages of Fruit Maturity
Provedor de dados: 52
Autores: Ahmad,Suheel; Sultan,Sheikh M.
The objective of present study was to determine appropriate fruit harvesting stage for Jatropha curcas L. for getting seeds with good oil content and better germination. The seeds separated from the fruits harvested at yellow capsule stage (C2) showed maximum germination efficiency, both under laboratory and nursery conditions whereas seeds separated from the fruits harvested at brownish black stage (C3) yielded highest oil content. Yellow capsule stage (C2) was physiologically mature fruit stage, which should be harvested to obtain the seeds with higher oil content besides showing maximum germination efficiency.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jatropha curcas; Capsule maturity; Physiological changes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100118

Seasonal variation in fish larvae at the entrance of Guanabara Bay, Brazil
Provedor de dados: 52
Autores: Castro,Marcia S. de; Bonecker,Ana Cristina Teixeira; Valentin,Jean L.
Studies were carried out to find out qualitative and quantitative temporal variations in fish larvae to relate seasonal, diurnal and tidal cycles to the density of larvae. Sampling was performed at one station, during two three-day periods in September 1995 and March 1996. Samples were collected during ebb and flood tides,in oblique hauls using a bongo net of 500 µm mesh size. A total of 42 taxa were identified. The families Engraulidae (Cetengraulis edentulus, Anchoa lyolepis) and Clupeidae (Harengula jaguana) had high mean densities and dominated both periods. Higher larval mean densities occurred in March, a period of relatively high temperatures and low salinities. The diurnal pattern of larval abundance consisted of high densities at night and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish larvae; Seasonal variation; Tidal cycle; Diurnal variation; Coastal bay; Guanabara Bay.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100016

Influence of gastrointestinal system conditions on adhesion of exopolysaccharide-producing Lactobacillus delbrueckii subsp. bulgaricus strains to caco-2 cells
Provedor de dados: 52
Autores: Darilmaz,Derya Onal; Aslım,Belma; Suludere,Zekiye; Akca,Gülcin.
This study aimed to assess the transit tolerance of potential probiotic dairy Lactobacillus strains in human uppergastrointestinal tract in vitro, and to evaluate the effect of EPS production on the viability and adhesion of these strains. Survival and adhesion of two exopolysaccharide (EPS)-producing L. delbrueckii subsp. bulgaricus strains (B3 and B2) and E. coli ATCC11229 were assessed after the exposure of different pH (gastric juice) and gastric plus pancreatic juice challenges. In the artificial gastric juice (pH 2), both the viability of the strain B3 and B2 was decreased. Artificial juice treatments significantly reduced the adhesion to caco-2 cells (P&lt; 0.05). High EPS-producing B3 survived better in the adverse gastrointestinal conditions and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Exopolysaccharide; Caco-2 Cells; Gastric-pancreatic juices; Lactobacillus delbrueckii subsp. bulgaricus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500009

Effect of inclusion of citrus pulp in the diet of finishing swines
Provedor de dados: 52
Autores: Watanabe,Pedro Henrique; Thomaz,Maria Cristina; Ruiz,Urbano dos Santos; Santos,Vivian Maia dos; Fraga,Alessandro Luís; Pascoal,Leonardo Augusto Fonseca; Silva,Susana Zaneti da; Faria,Henrique Gonzáles de.
The aim of this work was to study the effect of inclusion of citrus pulp in the diet of finishing pigs. In the first trial, 16 Topigs barrows with 80.5±4.7 kg of initial BW were used to evaluate the digestibility of citrus pulp. Having 18.85% of neutral detergent fiber and 41.18% of neutral detergent soluble fiber, citrus pulp showed as a feasible alternative ingredient to be added in the diets of feed restricted finishing pigs. Second trial tested the use of 0, 10, 20, 30% of citrus pulp in the diets of 36-feed-restricted barrows with initial BW of 83.7±5.1 kg. A quadratic trend (P&lt;0.05) as function of citrus pulp levels was observed for daily weight gain and number of experimental days to reach 130kg BW, being the best estimated inclusion levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative feedstuffs; Digestibility; Energetic restrictions; Heavy swine; Soluble fiber.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300028

Variações na estrutura da comunidade zooplanctônica próxima a usina I da Central Nuclear Almirante Álvaro Alberto (C.N.A.A.A.) - (Angra Dos Reis-R.J.-Brasil) - Ciclo 1980/81 E 1991/93
Provedor de dados: 52
Autores: Dias,Cristina de Oliveira; Bonecker,Sérgio L.C; Nogueira,Catarina R.
During the years of 1980/80 was performed a ecological study in Ribeira Bay (Angra dos Reis-R.J.-Brazil) in order to provide informations about of the planktonic community in pre-operational conditions, in the region of the Admiral Álvaro Aberto Nuclear Power Plant (C.N.A.A.A.). After 10 years of activities of the Nuclear Power Plant the zooplankton material was obtained in vertical hauls with a net of 150 μm at two fixed points (Piraquara de Fora Cove and Itaorna Beach) in order to study the zooplankton associations related to environmental variables and comparing with the anterior study. The zooplankton evolution showed a sazonal variation with the dominance of the holoplanktonic organisms. The Copepoda showed its the greatest growth during the autumn...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zooplâncton; Rio de Janeiro; Usina Térmica; Sazonal Variation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200013

In vitro antimicrobial activity and characterization of mangrove isolates of streptomycetes effective against bacteria and fungi of nosocomial origin
Provedor de dados: 52
Autores: Das,Arijit; Bhattacharya,Sourav; Mohammed,Abuelgasim Yegoup Hassan; Rajan,Subbaramiah Sundara.
The study aimed at determining the in vitro antimicrobial activity of alkaliphilic and halotolerant actinomycetes isolated from a mangrove ecosystem and identification of a potent strain. Twenty five isolates of actinomycetes were isolated from the sediment samples of Valapattanam mangrove swamp in Kerala, India. Antimicrobial activity of four selected actinomycete isolates was determined against bacterial and fungal pathogens of nosocomial origin by agar well diffusion method. Molecular characterization of the potent isolate was performed by 16S rDNA sequencing. Isolate no I-1 significantly inhibited Staphylococcus aureus ATCC 25923 (12 mm), S. aureus (15±0.05 mm), S. citreus (20±0.5 mm), Bacillus cereus (17±0.2 mm) and Serratia marcescens (12 mm). It...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomyces xiamenensis; Actinomycetes; Antimicrobial activity; Mangrove swamp.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300006

A modified method for the turbidimetric assay of nisin
Provedor de dados: 52
Autores: Flôres,Simone Hickmann; Braga,Ana Luíza de Mattos; Alegre,Ranulfo Monte.
Nisin is an antibacterial peptide produced by Lactococcus lactis subsp. lactis that shows a broad spectrum of inhibitory activity against gram-positive microorganism including bacterial spores. Methods for nisin activity estimation in solution is redefined. In this work a new turbidimetric assay method has been described making possible to assay nisin satisfactorily in total incubation period of approximately six hours.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lantibiotic; Nisin; Lactococcus lactis; Turbidimetric assay.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300020

Comparison of chemical constituents of Chamomilla recutita (L.) rauschert essential oil and its anti-chemotactic activity
Provedor de dados: 52
Autores: Presibella,Mayra Marinho; Villas-Bôas,Larissa De Biaggi; Belletti,Klézia Morais da Silva; Santos,Cid Aimbiré de Moraes; Weffort-Santos,Almeriane Maria.
Several essential oil samples from dried flower-heads of Chamomilla recutita (L.) Rauschert, grown and commercially available around Curitiba metropolitan area, South of Brazil, were analyzed by GC-MS and the chemical constituents were compared with an Egyptian sample obtained under similar conditions and used as a control. The local grown herbs showed levels of essential oil below the standards recommended by the Brazilian Pharmacopoeia. Also, differences in their composition as well as in the quantity of several components were found such as the unexpected inversion of the relative constitution of the A and B alpha-bisabolol oxides. Of particular interest was the striking effect of the chamomile extracts upon human leukocyte chemotaxis, a biological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chamomilla recutita; Asteraceae; Essential oil; Chemotaxis; Bisabolol oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600005

Influence of different carbon sources on exopolysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus (B3, G12) and Streptococcus thermophilus (W22)
Provedor de dados: 52
Autores: Yuksekdag,Zehra Nur; Aslim,Belma.
Exopolysaccharides (EPSs) production was studied by Lactobacillus delbrueckii subsp. bulgaricus (B3, G12) and Streptococcus thermophilus (W22) in the medium containing various carbon sources (glucose, fructose, sucrose or lactose). For all the strains, glucose was the most efficient carbon source and B3, G12 and W22 strains produced 211, 175 and 120 EPS mg/L respectively. Also, the influence of different concentrations of glucose (5,10,15,20,25,30 g/L) on EPS production and growth was studied. The results indicated that EPS production and growth were stimulated by the high glucose concentration (30 g/L).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus delbrueckii subsp. bulgaricus; Streptococcus thermophilus; Exopolysaccharide production; Carbon sources.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300019

Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds
Provedor de dados: 52
Autores: José,Anderson Cleiton; Silva,Edvaldo Aparecido Amaral da; Davide,Antonio Claudio; Toorop,Peter.
The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desiccation tolerance; Forest seed; Legumin; Proteomics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300006

Modelling and Optimisation of Stabilisation Ponds System for the Treatment of Swine Wastes: organic Matter Evaluation
Provedor de dados: 52
Autores: Costa,Rejane Helena Ribeiro da; Medri,Waldir.
O modelo de produção adotado na suinocultura brasileira, principalmente na Região Oeste de Santa Catarina, é feita com os animais confinados em pequenas áreas, trazendo como conseqüência grande produção de dejetos. Este é diluído, através do uso de água para a limpeza das baias, aumentando seu volume. Seu lançamento nos cursos d’água da região está comprometendo a qualidade dos mananciais devido a contaminação bacteriológica e poluição por compostos químicos. Dessa forma, há necessidade de processos de tratamento para minimizar os problemas ambientais gerados por essa atividade. As lagoas de estabilização têm sido utilizadas, por apresentar um excelente desempenho quanto à eficiência de remoção da matéria orgânica, dos nutrientes e de coliformes fecais;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stabilisation ponds; Swine wastes; Costs; Models; Optimisation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300017

In vitro Antiproliferative and inhibition of oxidative DNA damage activities of n-butanol extract of Limonium bonduelli from Algeria
Provedor de dados: 52
Autores: Amrani,Amel; Lahneche,Amina Maya; Benaissa,Ouahiba; Boubekri,Nassima; Demirtaş,Ibrahim; Benayache,Fadila; Benayache,Samir; Zama,Djamila.
Abstract Plants are the main sources of natural antioxidants in the form of phenolic compounds, which help human beings to deal with oxidative stress, caused by free radical damage. For this reason, the present study was carried out to evaluate the antiproliferative, antioxidant and inhibition of oxidative DNA damage activities of n-butanol extract obtained from aerial parts of Limonium bonduelli. The antioxidant potential was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and inhibition of lipid peroxidation assay. Antiproliferative activity was evaluated using xCELLigence RTCA instrument on two tumor cell lines; HT-29 (human colon adenocarcinoma) and HeLa (human cervix carcinoma). DNA damage inhibition was evaluated using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Limonium bonduelli; Antioxidant activities; Antiproliferative activity; DNA protection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100400

Spatial and temporal variation in the canopy cover in a tropical semi-deciduous forest
Provedor de dados: 52
Autores: Bianchini,Edmilson; Pimenta,José A.; Santos,Flavio A. M. dos.
This study was developed in the Mata dos Godoy State Park (23° 27’S, 51° 15’W) South Brazil which present great environmental heterogeneity when its north portion (ridge) is compared with the southern portion (valley), where there are some flooded areas. The forest formation is classified as tropical semi-deciduous forest. Spatial and temporal variations in the degree of the canopy cover were compared at flooded and unflooded sites. The measures were taken seasonally in 100m² plots using a spherical densiometer from spring 1995 to winter 1996. The flooded area was more open and lighter and had a greater spatial and temporal variation in the canopy cover than the unflooded areas. The greater heterogeneity in the flooded area could be due to structural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canopy; Flooding; Seasonality; Spatial heterogeneity; Tropical forest.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300008

Isolation of diuron-degrading bacteria from treated soil
Provedor de dados: 52
Autores: Dellamatrice,Priscila Maria; Monteiro,Regina Teresa Rosim.
Studies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period. In the first soil there was a significant increase in the number of bacteria, from 3.3 x 10 6 to 1.9 x 10(8). The microbial biomass did not change, however, a significant 14C-residue of diuron was found in the microbial biomass. A consortium of three bacteria, Acinetobacter johnsonii and two Bacillus spp., was isolated in medium containing diuron as the only carbon source. Only A. johnsonii was able to grow alone in medium with diuron as the only carbon source.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Biomass; Enhanced degradation; Diuron; Acinetobacter; Bioremediation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020

Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury
Provedor de dados: 52
Autores: Ishikawa,Nilton Massuo; Ranzani-Paiva,Maria José Tavares; Lombardi,Julio Vicente; Ferreira,Cláudia Maris.
Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Heavy metal pollution; Fish; Oreochromis niloticus; Hematology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400007

β-Glucanase Addition in Brewing Malt Produced by Reduced Time of Germination
Provedor de dados: 52
Autores: Brazil,Crislane; Oliveira,Débora Francielly de; Duarte,Rafael Augusto; Galo,Juliana Minardi; Lucchetta,Luciano; Santos,Eder da Costa dos; Hashimoto,Elisabete Hiromi.
Abstract The β-Glucans content has straight influence on the quality of malt and beer, mainly during the filtration step. Barley presenting high β-Glucan content demands longer germination time at malting. The application of commercial β-Glucanase is an alternative to accelerate the process and preserve the quality of malt. This work aimed to evaluate the effect of commercial β-Glucanase addition in malt produced within reduced germination time (64 h). Micro-malting was conducted with BRS-Caue and Elis barley cultivars at germination time 64 h and 96 h. The β-Glucanase concentration applied were 0, 25, 50 and 100 mg.kg-1. Barley, malt and wort samples were analyzed to check their physical-chemical features. Beers were produced with BRS-Caue malt and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-glucans; Germination time; Commercial enzyme.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100222

Feeding of Acestrorhynchus lacustris (Characidae): a post impoundment studies on Itaipu reservoir, upper Paraná River, PR
Provedor de dados: 52
Autores: Hahn,Norma Segatti; Delariva,Rosilene Luciana; Loureiro,Valdirene Esgarbosa.
With objective to know feeding spectrum of Acestrorhynchus lacustris (Reinhardt, 1874), a middle size predator in different places of influence area of Itaipu reservoir, stomach contents were analyzed. Samples were collected from March 1984 to February 1989 from Itaipu Reservoir and its adjacent areas. Its fed mainly on fishes, but it was an opportunistic (consumed 17 species of prey). Despite of the wide feeding spectrum, few preys were dominant in the diet, according to the different habitats sampled. As a result, it significant spatial differences in the diet was observed. Was observed through "Detrented Correspondence Analysis" (DCA) these were divided into three groups in relation with diet: preys from upstream, with higher scores; preys from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding; Predation; Characidae; Upper Paraná River; Itaipu Reservoir.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200010

Dissolved Nitrogen and Phosphorus Dynamics in the Lower Portion of the Paraiba do Sul River, Campos dos Goytacazes, RJ, Brazil
Provedor de dados: 52
Autores: Silva,Mônica Aparecida Leite; Calasans,Claudia Ferreira; Ovalle,Alvaro Ramon Coelho; Rezende,Carlos Eduardo.
The Paraíba do Sul river lower reach was monitored monthly between August 1995 and July 1996. This study was aimed at evaluating the temporal dynamics of dissolved nitrogen and phosphorus and its main controlling factors. Minimum and maximum observed values were as follows: N-NO2- - 0.08/0.51; N-NO3- - 21/57; N-NH4+ - 1.4/6.7; DON - 4.9/86.0; DIN -24.5/60.9; P-PO4(3-) - 0.43/1.66; DOP - 0.05/0.92; pH - 6.2/7.8; Dissolved Oxygen - 6.4/10.1; Conductivity - 48/74; Temperature - 20.5/31.1 (Nutrients - µM; Dissolved Oxygen - mg/l; Conductivity - µS/cm; Temperature - °C). Discharge presented a characteristic seasonal variation, showing a peak in January. Increasing P-PO4(3-), DOP, N-NH4+ and N-NO2- concentrations with increasing discharges could be associated to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dissolved nutrients; Paraíba do Sul River; Temporal dynamics; Nitrogen; Phosphorus; Discharge.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400006

Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats
Provedor de dados: 52
Autores: Açucena,Maria Kadja Meneses Torres; Pereira,Kércia Regina Santos Gomes; Villarim Neto,Arthur; Rêgo,Amália Cínthia Meneses; Bernardo-Filho,Mario; Azevedo,Ítalo Medeiros; Araújo Filho,Irami; Medeiros,Aldo Cunha.
This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p&lt;0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p&lt;0.05) of splenectomized animals, a significant increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Splenectomy; Biodistribution; Technetium; Rats.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700032

Phosphorus collectors from filter paper and synthetic cloth coated with iron or aluminium oxide to provide phosphorus by diffusion in soils
Provedor de dados: 52
Autores: Luchese,Eduardo Bernardi; Lenzi,Ervim; Favero,Luzia Otília Bortotti; Loubak,Luceide Heloisa.
Phosphorus collectors made from filter paper and synthetic cloth, were tested to evaluate their feasibility of determining the need for phosphate application. The collectors were coated with two types of oxides, iron oxide and aluminium oxide. The capacity of the collectors was tested by placing them in a 2 mL of solution containing phosphorus (PO4(3-)) in the concentration of 0.0, 1.00, 3.00, 5.00, 7.00, 9.00 and 11.00 µ g.mL-1, respectively, after which they were placed in contact with four types of soil (LBa, LRd, LEd and Ca) and incubed for 0.0 and 24 h. In this test the soils were kept at a humidity equivalent to 150 mmHg suction. The amount of phosphorus extracted from the solutions was tested at intervals between 0,0 and 11.0 µ g of phosphorus/mL....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphorus; Phosphorus availability; Phosphorus diffusion; Phosphorus extraction.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200006

Somatic embryogenesis in cassava genotypes from the northeast of Brazil
Provedor de dados: 52
Autores: Feitosa,Terezinha; Bastos,João Luíz Pinheiro; Ponte,Luíz Ferreira Aguiar; Jucá,Thiago Lustosa; Campos,Francisco de Assis de Paiva.
A method for the induction of somatic embryogenesis in eight cassava genotypes from northeastern Brazil is described. The explants used were shoot apexes isolated both from in vitro grown plants and from shoots that sprouted from stem cuttings. Somatic embryogenesis was achieved in high frequencies by the addition in the induction medium of the auxin picloram over a wide range of concentrations. Green cotyledons of primary somatic embryos were used as explants to induce somatic (cyclic) secondary embryogenesis in an inducing medium supplemented with picloram at 12 mg/L. The method could be used not only for the mass production of plants of the cassava genotypes, but also to generate explants (green cotyledons of somatic embryos) as themselves excellent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Cassava; Somatic embryogenesis; Plant tissue culture; Picloram.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200004

Microhardness and Ultrastructure of Demineralized Gamma-Irradiated Human Enamel after Diode Laser (980 nm) and Fluoride Surface Treatment
Provedor de dados: 52
Autores: Bakr,Mostafa AbdAllah; Saafan,Ali Mohamed; Maghraby,Eman Mohamed Fathy El; Rouby,Dalia Hussien El; Ahmed,Salwa Farid.
Abstract Quality of life drastically diminished after radiotherapy due to radiation induced oral complications. Fluoride was found to be helpful in decreasing the incidence of radiation caries; however it has not led to elimination of dental caries. Thus, new techniques containing low fluoride concentration or not containing fluoride at all, as laser irradiation, have been studied to prevent the beginning or progression of caries. So the purpose of this study is to investigate the effect of laser surface treatment with or without fluoride on microhardness and ultrastructure of demineralized gamma irradiated enamel; Thirty enamel slabs were allocated into three groups (n=10): G slabs were subjected to gamma irradiation only; GL slabs were subjected to gamma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laser; Fluoride; Gamma irradiation; Demineralization; Microhardness.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100606

Smart Health Surveillance with Automated Database Using Android Mobile Device
Provedor de dados: 52
Autores: Karthi,Anitha; Rajendran,Rajkamal; Mathiarasan,Poomanirajan.
ABSTRACT Smart Health Surveillance system is to measure and display the Electrocardiogram (ECG) and temperature of patient’s body continuously and also to communicate to the doctor. The system measures ECG using infrared sensor and the temperatures at oral and wrist of the patient using temperature sensors. Microcontroller, receives the data from the sensors, displays the same and communicates to the web server automatically. In the existing system, patient’s vital parameters are obtained and the obtained values are entered into database and then uploaded into a web-based server manually. The existing system has no alert signal, during abnormal condition to the surrounding and to the doctor. The proposed system consists of a visualization module of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Android mobile device; Health Surveillance; PIC microcontroller; Wireless sensor network.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100605

Computational Model for Microgeneration Simulation, From Solar and Wind Renewable Sources, With Optimal Allocation of Loads, Electric Vehicle and Energy Storage, In a Residential Electrical Micro Network
Provedor de dados: 52
Autores: Tiem,Wesley Thiago Egea; Unsihuay-Vila,Clodomiro.
Abstract The electrical sector is under constant evolution. One of the areas refers to the consumers that come to be generators, implementing distributed generation, interconnected to a smart grid. This article discusses the improvement of an algorithm, already presented in the literature, to make the best temporal allocation of loads, electric vehicle, storage and many sources of generation, aiming at the maximum financial performance, that is, the lowest value for the energy invoice The modeling consists of a Mixed Integer Linear Programming (MILP) algorithm, which considers each component of the system and weighs the maintenance and shelf life of storage devices, basically batteries, loads that can be reallocated and the concept of Vehicle-to-grid,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microgrid; Microgeneration; Distributed generation; Wind energy; Solar energy; Load reallocation; Storage; Electric vehicle; Smart grid.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200215

Seedling growth of understorey species of a Southeast Brazilian tropical forest
Provedor de dados: 52
Autores: Válio,Ivany Ferraz Marques.
Thirteen understorey species of a mesophyllous tropical forest were studied under two different photosynthetic photon flux densities (PPFD). Seedlings were grown in the glasshouse under 51% and 2.6% solar PPFD. Growth of the seedlings was evaluated by 1-) total height; 2-) leaf number; 3-) leaf dry mass;4-) stem dry mass; 5-) root dry mass; 6-) stem length/mass; 7-)shoot/root mass; 8-) percent allocation to leaf, stem and roots. For most of the parameters recorded, low PPFD drastically reduced growth. It seemed that the strategy of these understorey species was a reduction of growth under low PPFD saving energy for survival.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seedling growth; Understorey species; Shade; Tropical forest.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400024

Dissolution profile evaluation of solid pharmaceutical forms containing chloramphenicol marketed in Brazil
Provedor de dados: 52
Autores: Ferraz,Humberto Gomes; Carpentieri,Leticia Norma; Watanabe,Sayuri Pereira.
The dissolution profile for solid pharmaceutical forms containing chloramphenicol 250 mg available in Brazil was determined using a method from the American Pharmacopoeia (United States Pharmacopoeia, 2004) and then compared. Two different methods of dissolution profile comparison were used: ANOVA, and an independent model. Differences between the formulations were reflected in the dissolution profiles. The presence of metastable polymorphs or amorphous forms of chloramphenicol palmitate might be responsible for variations in the concentration of the drug observed within formulations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloramphenicol; Dissolution; Polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100007

Natural Diet of Callinectes ornatus Ordway, 1863 (Decapoda, Portunidae) in the Itapocoroy Inlet, Penha, SC, Brazil
Provedor de dados: 52
Autores: Branco,Joaquim Olinto; Lunardon-Branco,Maria José; Verani,José Roberto; Schveitzer,Rodrigo; Souto,Flávio Xavier; Vale,William Guimarães.
From January to December 1995, 332 individuals of the Callinectes ornatus species were collected from the Itapocoroy inlet in Penha, Sta. Catarina, Brazil to study its natural diet and the seasonal variations of diet. Results showed a diversified trophic spectrum with a generalized dietary strategy comprising the algae, macrophyta, foraminiferida, mollusca, polychaeta, crustacea, echinodermata, Osteichthyes and NIOM (Nonidentified Organic Matter) groups.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. ornatus; Portunidae; Natural diet; Dietary habits; Dietary ecology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100006

Effect of proteins from the red seaweed Hypnea musciformis (Wulfen) Lamouroux on the growth of human pathogen yeasts
Provedor de dados: 52
Autores: Cordeiro,Rossana Aguiar; Gomes,Valdirene Moreira; Carvalho,Ana Fontenele Urano; Melo,Vânia Maria Maciel.
A protein fraction, rich in lectin, obtained from the red seaweed Hypnea musciformis by precipitation with ammonium sulfate (F40/70) was screened for chitinase and beta-1,3-glucanase activity and assessed for antifungal potential against the human pathogen yeasts Candida albicans and C. guilliermondii. The F40/70 fraction showed chitinase and beta-1,3-glucanase enzymes, with specific activities of 276.43 and 1880.7 Units.mg -1 protein, respectively. It was capable of inhibiting the growth of C. guilliermondii at the concentrations of 45, 100 and 450 µg protein.ml -1 but it showed only a discrete inhibition against C. albicans irrespective of the tested concentrations. The inhibitory action was shown to be fungistatic and the presence of the glycoprotein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypnea musciformis antifungal activity beta-1; 3-glucanase chitinase lectin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700008

Predaceous ant fauna in new sugarcane fields in the state of São Paulo, Brazil
Provedor de dados: 52
Autores: Rossi,Marcelo Nogueira; Fowler,Harold Gordon.
Predaceous ant fauna present in natural sugarcane field plantations in the state of São Paulo, Brazil, was evaluated by using sardine baits sampling technique. Three-month-old sugarcane plants were used for ant fauna estimation in two sugarcane mills, São João and Barra Mill. Twelve 30m X 30 m (900 m²) plots were previously delimited in each sugarcane mill for ant sampling. Ants were sampled in each plot by placing nine sardine baits in 12 mm X 75 mm plastic tubes. In the São João Mill, the predominant ant species observed was Solenopsis saevissima, followed by Dorymyrmex sp. 1, Pheidole sp. 2, and Crematogaster sp. 1. Considering only ant genus, Solenopsis, Pheidole, Dorymyrmex, and Crematogaster, were predominant. In the Barra Mill, the predominant ant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ants; Predation; Diatraea saccharalis; Sugarcane; Solenopsis; Pheidole.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500017

Tc-99m direct radiolabeling of monoclonal antibody ior egf/r3: quality control and image studies in mice
Provedor de dados: 52
Autores: Dias,Carla Roberta; Marczewski,Barbara; Moraes,Vanessa; Barboza,Marycel Figols de; Osso Junior,João Alberto.
Monoclonal antibodies (Mabs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in the practice of nuclear medicine. The ior egf/r3 (Centis, Cuba) is a murine monoclonal antibody against epidermal growth factor receptor (EGF-R) and has been widely used in the radioimmunodiagnosis of tumors of epithelial origin. Labeled with 99mTc, its main application in Nuclear Medicine is the follow up, detection and evaluation of tumor recurrences. The objective of this work is to describe the preparation of a lyophilized formulation (kit) for radiolabeling the Mab ior egf/r3 with 99mTc for immunoscintigraphic applications. Radiolabeling efficiency, effects on immunoreactivity, image studies and stability of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiolabeling; Technetium; Monoclonal antibodies; Nuclear medicine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700004

Water potential and viability of seeds of Eugenia (Myrtaceae), a tropical tree species, based upon different levels of drying
Provedor de dados: 52
Autores: Delgado,Liliana Ferreira; Barbedo,Claudio José.
The aim of the present work was to study the water potential and viability of Eugenia based upon different levels of drying. The seeds showed high water content at shedding and were sensitive to desiccation; however, the level of this sensitivity (water content) varied among the species. Five target drying levels were defined according to the critical levels of hydration. Results showed that water potential seemed to be more reliable than water content. It was hypothesize that the differences in desiccation tolerance, as well as recalcitrance, among the seeds of different species could be related to the maturity level of these seeds during shedding from the mother plant. Consequently, this could be a result of the evolution on how far the maturation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desiccation tolerance; Drying; Seed water relations; Recalcitrance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400014

Aspects on the catalysis of lipase from porcine pancreas (type VI-s) in aqueous media: development of ion-pairs
Provedor de dados: 52
Autores: Kokkinou,Marina; Theodorou,Leonidas G.; Papamichael,Emmanuel M.
This article reports a first contribution for the elucidation of catalytic mechanism of Lipase from porcine pancreas, type VI-s (PPL), in hydrolyzing an ester substrate in aqueous media. The conclusions were based on the pH-profiles of Michaelis-Menten parameters k cat/Km, k cat and Km, as well as on the absolute temperature profile of k cat/Km, obtained during the hydrolysis of p-nitrophenyl laurate by PPL. It was found that (a) PPL performs catalysis by means of ion pairs formed either as Ser152-Ο-/His263-Im+H and/or Carbonyl-Ο-/His263-Im+H, (b) the parameter k cat/Km equals to k1 and thus ES is formed and destroyed in the course of a series of consecutive reactions governed by the dynamic constant K S = k2/k1, and (c) the hydrolysis of substrate is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Porcine Pancreas Lipase; Mechanism of hydrolysis; P-nitrophenyl laurate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200007

A ictiofauna da bacia do Rio Paraíba do Sul. Biodiversidade e padrões biogeográficos
Provedor de dados: 52
Autores: Bizerril,Carlos Roberto S. Fontenelle.
Fish fauna taxonomic composition and biogeographic informations of the Paraíba do Sul river basin are presented. Samples were taken in 75 units between January/1995 and June/1998. Species were identified and classified according its distributional pattern. Biogeographic analyses were conduced using macro, meso and micro scales. A total of 167 species were sampled, being 37 marine species. Among the marine taxa, Awaous tajasica and Oosthetus lineatus seems to spend their whole life histories in the freshwater environments. Sixteen species were introduced in the basin. Most of the native taxa inhabit the rivers’storage zone, mainly in pools. Faunistic similarities between the Paraíba do Sul river basin and other Brazilian rivers suggest that the environment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; Paraíba do Sul river basin; Freshwater fishes; Southeastern Brazil.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200014

Spore Germination and Protonemal Development of Dolichomitriopsis diversiformis
Provedor de dados: 52
Autores: Liu,Shibiao; Wei,Hua; Peng,Xiaolie; Li,Jing.
ABSTRACT Dolichomitriopsis diversiformis is an endemic and threatened moss in eastern Asia. In vitro culture and light microscopic observation were employed to study its developmental process from spore germination to the formation of young gametophyte, as well as effects of light and temperature on its spore germination and protonemal development. Microscopic observations revealed that its spores were positively photoblastic with exosporous germination, and sporelings were classified as the bryum-type. Light and dark conditions were compared to understand their effects on spore germination. In 24 h continuous illumination, all spores germinated in both 20 ℃ and 25 ℃. In contrast, in darkness at 20 ℃ from one to 30 days, spores did not germinate. However,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dolichomitriopsis diversiformis; Spore; Protonema; Light; Temperature.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100202

Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
Provedor de dados: 52
Autores: Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz.
Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adventitious bud; Anatomy; Morphogenesis; Zingiberaceae.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200014

Immobilized Biofilm in Thermophilic Biohydrogen Production using Synthetic versus Biological Materials
Provedor de dados: 52
Autores: Wongthanate,Jaruwan; Polprasert,Chongrak.
Biohydrogen production was studied from the vermicelli processing wastewater using synthetic and biological materials as immobilizing substrate employing a mixed culture in a batch reactor operated at the initial pH 6.0 and thermophilic condition (55 ± 1ºC). Maximum cumulative hydrogen production (1,210 mL H2/L wastewater) was observed at 5% (v/v) addition of ring-shaped synthetic material, which was the ring-shaped hydrophobic acrylic. Regarding 5% (v/v) addition of synthetic and biological materials, the maximum cumulative hydrogen production using immobilizing synthetic material of ball-shaped hydrophobic polyethylene (HBPE) (1,256.5 mL H2/L wastewater) was a two-fold increase of cumulative hydrogen production when compared to its production using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biohydrogen production; Biofilm; Synthetic material; Biological material; SEM.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100124

Evaluation of apple pomace based reconstituted feed in rats after solid state fermentation and ethanol recovery
Provedor de dados: 52
Autores: Devrajan,A.; Joshi,Vinod K.; Gupta,Kuldeep; Sheikher,Chander; Lal,Brij B.
To utilize apple pomace in an economical and effective way, a feed was developed by solid state fermentation (SSF) using sequential interactive co-culture of Candida utilis and Kloeckera. Removal of ethanol and drying of the left - over residue. Feeding trial was conducted in white albino rats before and after reconstitution of apple pomace feed in the choice and no choice study. Feeding of apple pomace feed in the rats before reconstitution indicated that neither in fermented nor in unfermented form it was acceptable. In the no choice study both in 100% fermented and unfermented apple pomace group feed intake decreased continuously resulting in death of rats apparently due to decreased digestibility owing to high fibre content. Further, fermented or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple pomace; Rat feed; Solid state fermentation; Ethanol.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100013

Antioxidant properties of Spirulina (Arthospira) platensis cultivated under different temperatures and nitrogen regimes
Provedor de dados: 52
Autores: Colla,Luciane Maria; Furlong,Eliana Badiale; Costa,Jorge Alberto Vieira.
The main aim of this work was to investigate the effects of temperature and nitrogen concentration on the antioxidant potential of extracts from Spirulina (Arthospira) platensis biomass. S. platensis biomass obtained at 35ºC and with 1.875 g.L-1 or 2.5 g.L-1 of sodium nitrate in the culture medium presented higher concentrations of phenolic compounds. The antioxidant potential of methanol extracts of biomass on the enzymatic browning caused by peroxidase were 29 and 35%, respectively, being the reduction related to the amount of phenolic compounds present in this extract.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Phenolic compounds; Spirulina (Arthospira) platensis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100020

Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells
Provedor de dados: 52
Autores: Paoli,Severo de; Giani,Tania Santos; Presta,Giuseppe Antonio; Pereira,Marcia Oliveira; Fonseca,Adenilson de Souza da; Brandão-Neto,José; Medeiros,Aldo da Cunha; Santos-Filho,Sebastião David; Bernardo-Filho,Mário.
Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p&lt;0.05) the %ATI of blood constituents and the shape of red blood cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Blood constituents; Red blood cells; Morphology; Caryophyllus aromaticus L.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600022

Expression analysis of three immune genes Interferon-gamma, Mx and Interferon regulatory factor-1 of Japanese flounder ( Paralichthys olivaceus )
Provedor de dados: 52
Autores: Zhang,Feng; Qiu,Xuemei; Liu,Yang; Wang,Juan; Li,Xipeng; Wang,Xiuli.
ABSTRACT IFN-γ (Interferon-gamma), Mx and IRF-1 (Interferon regulatory factor-1) are main immune-related genes and they play important roles in the innate immune system of vertebrates. In this study, the expression level of the three immune-related genes in twelve tissues of normal adult Japanese flounder was detected using semi-quantitative RT-PCR. Thirteen time points (3h, 6h, 9h, 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, 108h, 120h) were selected to analyze the expression of IFN-γ, Mx and IRF-1 in spleen, head kidney, liver of Japanese flounder infected with Lipopolysaccharide (LPS). The Japanese flounder IFN-γ, Mx and IRF-1 genes were differently expressed in these tissues and had high expression levels in classical fish immune organs like spleen and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon-gamma; Mx; Interferon regulatory factor-1; Paralichthys olivaceus; Tissue expression.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100300

Cytogenetic and teratological effects of mancozeb pre natal exposure on rats
Provedor de dados: 52
Autores: Castro,V. L.; Tambasco,A. J.; Paraíba,L. C.; Tambasco,D. D.
Studies were conducted on the dam’s fertility and pup viability, litter physical and motor development and chromosomal analysis with female rats exposed to different concentrations of mancozeb, at initial and organogenesis periods of pregnancy. The exposure did not interfere with the pregnant females or their estrous cycle but resulted in retarded physical development, with exception of the vaginal opening and tests descent; which were in advance. The litter body weight was not affected. The examination of swimming performance indicated an initial impairment and a posterior recovery. The observed chromosome damage in dams and pups was found to enhance with the fungicide’s concentration. The results obtained led to the conclusion that even in the absence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mancozeb; Rats; Postnatal development; Prenatal exposure; Genetic evaluation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200001

Epidemiology Study on P53 (Rs1614984) C&amp;gt;T Mutation in Cigarette Smokers
Provedor de dados: 52
Autores: Ahmad,Dilshad.
ABSTRACT Epidemiology data have established that smoking is a prime threat for the cancers, largely lung cancer. Single-nucleotide polymorphisms (SNPs),P53 SNPs have been found to be associated with the predisposition of different cancers. Their decreased expression is reported in breast and lung cancer patients. p53 (rs1614984) had been reported to be linked with the SNPs found associated with breast cancer. The primary aim of this study to determine the association of p53 variant rs1614984 with the cigarette smokers and smoking related cancers in smokers. Among the smokers, 38% were found with CC genotype, 55% were heterozygous CT and 7% were TT, respectively. The homozygous TT genotype was seen in lower percentage of smokers (7%) when compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53 (rs1614984); Cigarette smokers; Lung cancer; Single nucleotide polymorphisms (SNPs).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100304

Organogenesis from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis -a vulnerable species of Western Ghats
Provedor de dados: 52
Autores: Lakshmi,Subbaiah Revathi; Benjamin,Jambarapu Herald Franklin; Kumar,Tirupathi Senthil; Murthy,Garimella Venkata Suryanarayana; Rao,Mandali Venkateswara.
An efficient system was developed for indirect plant regeneration from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis. Maximum percentage of the organogenic callus was obtained on MS medium supplemented with NAA (1.0 mg/l) and 2,4-D (2.0 mg/l). The best shoot bud induction was observed on MS medium with BA (1.0 mg/l) +IBA (0.5 mg/l). The coconut water (15%) was better, resulting in a differentiation of the shoot initials in to well-developed shoots. The elongated shoots (› 3cm long) were rooted on a full strength MS basal medium, supplemented with 0.2 mg/l of IBA. Finally, the rooted plants were transferred to the soil with 80% success rate. This protocol was utilized for the in vitro propagation of this endangered plant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro explants; Organogenesis; Hoya wightii ssp. Palniensis; Vulnerable plant; Growth regulators.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300010

Bioavailability of the sodium pertechnetate and morphometry of organs isolated from rats: study of possible pharmacokinetic interactions of a ginkgo biloba extract
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José; Nascimento,Ana Lúcia; Pereira,Mário; Rocha,Emely Kazan; Diré,Gláucio; Arnobio,Adriano; Caldas,Luiz Querino de Araújo; Bernardo-Filho,Mario.
Many compounds affect the bioavailability of radiobiocomplexes as radiopharmaceuticals. Ginkgo Biloba extract (EGb) has several effects. The influence of an EGb on the bioavailability of the radiobiocomplex sodium pertechnetate (Na99mTcO4) and on the morphometry of the organs was evaluated. Rats were treated with EGb and Na99mTcO4 was injected. The animals were sacrificed; the radioactivity in the organs was counted. The results showed that EGb altered the Na99mTcO4 bioavailability in the kidneys, liver and duodenum. Morphometric analysis of the organs showed significant alterations (P&lt;0.05), probably caused by metabolites generated by EGb and capable of altering the bioavailability of the Na99mTcO4.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgo biloba extract; Bioavailability; Radiobiocomplex; Sodium pertechnetate; Morphometry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700011

Molecular docking of Anopheles gambiae and Aedes aegypti glutathione S-transferases epsilon 2 (GSTE2) against usnic acid: an evidence of glutathione conjugation
Provedor de dados: 52
Autores: Maia,Rafael Trindade; Nadvorny,Daniela.
The aim of this study was to develop a theoretical model using Anopheles gambiae GSTE2 structure as template for Aedes aegypti GSTE2 by homology modeling Docking simulations were performed for both the enzymes against usnic acid in neutral and anionic forms. Ramachandran plot revealed that 93.9% of the GSTE2 model residues were located on most favored regions. Model evaluation was made by the ANOLEA and GROMOS analysis. Docking results indicated that the enzymes were able to form glutathione-conjugate with usnic acid in both the forms (anionic and neutral).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anopheles gambiae; Aedes aegypti; Molecular modeling; Insecticide resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500689

Targeting of Virulence Factors and Plasmid Profiling of Klebsiella pneumoniae Causing Urinary Tract Infection in Sylhet City of Bangladesh
Provedor de dados: 52
Autores: Foysal,Md Javed; Majlish,Al-Nahian Khan; Islam,Kamrul; Alam,Md Jahangir; Ali,Md Hazrat; Momtaz,Farhana.
ABSTRACT Studies were conducted to characterize Klebsiella pneumoniae isolates from urinary tract infection (UTI) patients in Sylhet city of Bangladesh. At the same time, all isolates were screened for some common virulence genes and four significant isolates were searched for plasmid number and sizes by mini alkaline-lysis method. Among five tested isolates from female UTI patients, gyrase subunit B2 (gyrb2) amplified in all isolates, lipase and nuclease detected in three isolates and serine protease amplifies in two isolates and gave the expected band of 1130 bp, 517 bp, 1055 bp and 211 bp respectively. Two of four isolates showed 9.82 kb plasmid band on agarose gel. Isolates bearing 9.82 kb plasmid were found to be resistant to multiple commercial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: K. pneumoniae; Urinary tract infection; Virulence factors; Plasmid profiling; Correlation study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100423

Metabolic and behavioral effects of ractopamine at continuous low levels in rats under stress
Provedor de dados: 52
Autores: Lopes,Edna; Sousa,Raimundo Vicente de; Zangeronimo,Márcio Gilberto; Pereira,Andressa Naira de Jesus; Coelho,Mariana de Resende; Ferreira,Matheus Soares da Silva; Lima,Renato Ribeiro; Marcondes,Fernanda Klein; Napimoga,Marcelo Henrique; Pereira,Luciano José.
This study aimed at evaluating the effect of ractopamine (RAC) on metabolism, zootechnical performance, body composition, and behavior in Wistar rats submitted to acute and chronic restrain stress. The oral dose of 5 mg/kg of RAC was administered in periods of 0, 7, 14, 21, and 28 days. The elevated plus-maze test (EPMT) was used for behavioral assessment. Blood, carcass and viscera characteristics were evaluated. Insulin-dependent glucose transporters (GLUT-4) were semi-quantified by Western Blot in epididymal adipocytes. RAC periods associated with chronic stress increased the GLUT-4 protein expression in adipose tissue in a time-dependent manner (P=0.01), i.e., the longer the RAC addition period, the higher the GLUT-4 concentration in chronically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-adrenergic agonist; Ractopamine; Zootechnical performance; Metabolism; Elevated Plus-Maze Tests.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300406

Design, construction, and analysis of specific zinc finger nucleases for microphthalmia - associate transcription factor
Provedor de dados: 52
Autores: Wang,Wenwen; Liu,Hongmao; Zhao,Haibin; Luo,Zaili; Shi,Yongxiang.
This work studied the design, construction, and cleavage analysis of zinc finger nucleases (ZFNs) that could cut the specific sequences within microphthalmia - associate transcription factor (mitfa) of zebra fish. The target site and ZFPs were selected and designed with zinc finger tools, while the ZFPs were synthesized using DNAWorks and two-step PCR. The ZFNs were constructed, expressed, purified, and analyzed in vitro. As expected, the designed ZFNs could create a double-stand break (DSB) at the target site in vitro. The DNAWorks, two-step PCR, and an optimized process of protein expression were firstly induced in the construction of ZFNs successfully, which was an effective and simplified protocol. These results could be useful for further application...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zinc finger nuclease; DNAWorks; Gene synthesis; Gene knockout.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400011

Variation and its distribution in wild cacao populations from the Brazilian Amazon
Provedor de dados: 52
Autores: Dias,Luiz Antônio dos Santos; Barriga,Júlio Pontes; Kageyama,Paulo Yoshio; Almeida,Caio Márcio Vasconcellos Cordeiro de.
A sample of 64 progenies (320 cacao trees as a whole) from four Brazilian Amazon basins was collected and evaluated on the basis of 15 fruit and seed traits. Nested univariate analyses of variance showed significant variation across progenies and basins. However, most of the variability appeared to be due to among trees and basins differences. The multivariate analysis showed that the differentiation in cacao populations occurred among basins. Since cacao diversity was predominantly found in trees within basins and among basins, one should optimize the collecting process by taking as many trees as possible starting from few progenies and many river basins. These findings seemed to validate gene conservation efforts made to date to preserve the cacao...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Theobroma cacao L.; Cacao trees; Genetic structure; Collecting wild germplasm; Amazon river basin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400003

Fish species diversity in headwaters streams of Paraguay and Paraná Basins
Provedor de dados: 52
Autores: Súarez,Yzel Rondon; Valério,Sabrina Bigatão; Tondado,Karina Keyla; Florentino,Alexandro Cezar; Felipe,Thiago Rota Alves; Ximenes,Lidiani Queli Lubas; Lourenço,Luzia da Silva.
The influence of spatial, temporal and environmental factors on fish species diversity in headwater streams in Paraguay and Paraná basins, Brazil was examined. A total of 4,605 individuals were sampled, distributed in 60 species. The sampled streams in Paraná basin presented a larger total species richness (42) than Paraguay streams (40). However the estimated richness was larger in Paraguay basin (53) than Paraná streams (50). The streams of Paraná basin had a greater mean species richness and evenness, while more individuals per sample were found in the Paraguay basin. Difference between the sub-basins were found in the Paraguay basin, while for the basin of Paraná, richness and evenness vary significantly between the sub-basins, but the number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community ecology; Fish diversity; Streams; Paraguay Basin; Paraná Basin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700014

Erratum: Antifungal Potential of Indigenous Medicinal Plants against Myrothecium Leaf Spot of Bitter Gourd (Momordica charantia L.)
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100904

Biomonitoring of Pb and Cd in two impacted watersheds in Southeast Brazil, using the freshwater mussel Anodontites trapesialis (Lamarck, 1819) (Bivalvia : Mycetopodidae) as a biological monitor
Provedor de dados: 52
Autores: Tomazelli,Andréa C.; Martinelli,Luiz A.; Avelar,Wagner E. P.; Camargo,Plínio B. de; Fostier,Anne-Helene; Ferraz,Epaminondas S. B.; Krug,Francisco J.; Santos Júnior,Dário.
Studies were carried out to investigate the contamination of Piracicaba and Mogi-Guaçu River basins (São Paulo State, Southeastern, Brazil) for heavy metals (Cd and Pb), using the limnic bivalve Anodontites trapesialis as a biological monitor. The results showed that the concentrations of Pb were higher than the control group in both basins, showing the bioavailability of this non-essential element in the basins. The concentrations were higher in the Mogi-Guaçu than in the Piracicaba basin, and in the slightly contaminated sites in both basins. There was no correlation between the degree of human impact and Cd and Pb concentrations, it was not possible to infer about concentrations of these heavy metals in the bivalves based only in a broad evaluation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomonitoring; Heavy metals; Mussels; Piracicaba; Mogi-Guaçu; Brazil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400022

Characterization of grid-connected photovoltaic systems in Curitiba, Brazil
Provedor de dados: 52
Autores: Tonin,Fabianna Stumpf; Urbanetz Junior,Jair.
ABSTRACT In the search for new options for the establishment of the energy matrix of Brazil, the Grid-Connected Photovoltaic Systems (GCPVS) are configured as an alternative to urban centers, because they allow the power generation at distributed mode, that is, generate energy at the place where it will be consumed and inject the surplus energy into the network. Faced with the prospect of installing Grid-Connected Photovoltaic System, the objective of this research is to characterize this type of photovoltaic system installed in Curitiba, State of Paraná, Brazil. This paper highlights that up to July 2017 the State of Paraná has 1031 GCPVS in operation, which corresponds to 6,6MW, while Curitiba has 204 GCPVS that represents 997.4 kW in operation, 82% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grid-Connected Photovoltaic Systems; Distributed Generation; Solar Energy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200221

Clotting and fibrinogenolysis inhibition by essential oils from species of the Asteraceae family
Provedor de dados: 52
Autores: Miranda,Cíntia Alvarenga Santos Fraga de; Cardoso,Maria das Graças; Marcussi,Silvana; Teixeira,Maria Luisa.
ABSTRACT Essential oils are pharmacologically active and unexplored compounds. The inhibitory properties of essential oils from Baccharis dracunculifolia, Conyza bonariensis, Tithonia diversifolia and Ambrosia polystachya were evaluated in the coagulation and fibrinogenolysis induced by snake venoms. The essential oil from Conyza bonariensis extended the clotting time of Lachesis muta from 52.2 to 115.2 seconds and that of Bothrops moojeni from 108.3 to 2340.0 seconds, when pre-incubated with the venoms. The longest clotting times for Bothrops atrox venom were observed after incubation with the essential oils from Conyza bonariensis and Tithonia diversifolia: the times increased from 100.8 to 264.0 and 227.7 seconds, respectively. The prior incubation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteases inhibitors; Baccharis dracunculifolia; Conyza bonariensis; Tithonia diversifolia; Ambrosia polystachya.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100327

Growth and survival of dorado Salminus brasiliensis (Pisces, Characidae) post-larvae cultivated with different types of food and photoperiods
Provedor de dados: 52
Autores: Schütz,Jackson Haroldo; Nuñer,Alex Pires de Oliveira.
The effect of food and photoperiod on dorado (Salminus brasiliensis) post-larvae growth and survival was investigated using the following treatments: LD (Light: Dark) 24:0 + Artemia nauplii (A), LD 24:0 + fish prey (F), LD 14:10 + A, LD 14:10 + F, LD 0:24 +A and LD 0:24 + F. Prochilodus lineatus larvae were used as fish prey. Higher survival rates were registered in fish prey treatments (P&lt;0.05) and were not influenced by photoperiod whereas in Artemia treatments, the survival was very low, but directly proportional to light increase (P&lt;0.05). The better growth in LD 0:24 was related to a safe-energy strategy that reduced energy consumption whereas in LD 24:0, the higher energy consumption reduced the growth. In intermediate photoperiod, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dorado; Food type; Larviculture; Photoperiod; Salminus brasiliensis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300010

Moving from organ dose to microdosimetry: contribution of the Monte Carlo simulations
Provedor de dados: 52
Autores: Champion,Christophe.
When living cells are irradiated by charged particles, a wide variety of interactions occurs that leads to a deep modification of the biological material. To understand the fine structure of the microscopic distribution of the energy deposits, Monte Carlo event-by-event simulations are particularly suitable. However, the development of these track structure codes needs accurate interaction cross sections for all the electronic processes: ionization, excitation, Positronium formation (for incident positrons) and even elastic scattering. Under these conditions, we have recently developed a Monte Carlo code for electrons and positrons in water, this latter being commonly used to simulate the biological medium. All the processes are studied in detail via...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electron; Positron; Monte Carlo Simulation; Microdosimetry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700029

Short-term variability of bacterioplankton in the maximum turbidity zone in the Paranaguá Bay, Southern Brazil, and its relationship with environmental variables
Provedor de dados: 52
Autores: Ribeiro,Catherine Gérikas; Kolm,Hedda Elisabeth; Costa Machado,Eunice da.
In this work, the density of bacterioplankton, bacterial biomass and environmental variables were monitored in two seasons (summer and winter), two times each month (spring tide and neap tide sampling), over a 12 h period, comprising a tidal cycle (semidiurnal), from subsurface and bottom waters, in a fixed station in the Estuarine Turbidity Maximum Zone (ETMZ) of Paranaguá Bay, Brazil. The data were treated with multivariate analyses methods in order to indentify the key controlling factors of the bacterial community dynamics. The microbial community seemed to be structured by a close relationship with the nutrients concentration, mainly by total phosphorous and nitrate. Regardless of variations throughout the tidal cycles, free-living bacteria had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterioplankton; Estuarine Turbidity Maximum Zone; Free-living bacteria; Attached bacteria; Paranaguá Bay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500012

Assessment of the plan for pre-exposition vaccination with Fuenzalida-Palacios anti-rabies vaccine
Provedor de dados: 52
Autores: Preto,Angela Aparecida; Gomes,Elisa Maines; Fernandes,Maurício José; Hennig,Nei Adolfo; Germano,Pedro Manoel Leal.
Studies ere carried out to monitor the titres of anti-rabies antibodies taken from individuals exposed to the risk of infection. Fifty-seven individuals aged between 20 and 40, were vaccinated with a minimum titre of 1.3 IU Fuenzalida-Palacios type anti-rabies vaccine. The vaccination plan consisted of the application of three doses at two day intervals and a booster applied 28 days after the final dose. The accompainment of the antibody titres was achieved by means of the seroneutralization test over a total period of 18 months. Through the monitoring carried out, it was found to be necessary to apply a booster vaccination to part of the group. Some of these individuals were revaccinated with Fuenzalida-Palacios type vaccine with a minimum titre of 1.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Rabies vaccine; Antibodies; Neutralization; Fuenzalida-Palacios.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300002

Sperathe effects of solid-state fermentation in the functional properties of defatted rice bran and wheat bran
Provedor de dados: 52
Autores: Silveira,Cristina Moreira da; Badiale-Furlong,Eliana.
Functional properties of fermented bran produced by Aspergillus oryzae and Rhizopus sp. in a solid-state fermentation system were determined, with an aim to evaluate their application in food formulation. The defatted rice bran and wheat bran were inoculated with the spores of the cultures and incubated at 30º C for 72 h. Samples were withdrawn at 0, 24, 48 and 72 h. Protein content, protein solubility, in-vitro digestibility, gelation and water holding capacity were determined in bran with or without fermentation. Rhizopus sp. increased significantly the protein content (69.0 and 56.0%, respectively, for defatted rice bran and wheat bran); protein solubility (28.5 and 36.2) and water holding capacity (11.4% for wheat bran). When A. oryzae was used all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional properties; Solid-state fermentation; Defatted rice bran; Wheat bran.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600027

Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation
Provedor de dados: 52
Autores: Benjamin,Sailas; Pandey,Ashok.
Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40ºC and pH 7-8....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500002

Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis
Provedor de dados: 52
Autores: Kapnoor,Shankar; Mulimani,Veerappa Hanumanth.
α-Galactosidase was produced by Aspergillus oryzae on red gram plant waste-wheat bran based media in solid-state fermentation (SSF). Optimum temperature for α-galactosidase production was 35 0C and upto 4 cm of bed height of substrate had no inhibitory effect on enzyme production. Hydrolysis of galactooligosaccharides in soymilk was carried out by α-galactosidase. Optimum temperature and pH for the hydrolysis of raffinose and stachyose of soymilk were 55(0)C and 5.2-6.2, respectively. The enzymatic treatment for 3 h completely removed the raffinose oligosaccharides in soymilk. Crude extract also showed considerable amount of invertase activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus oryzae; Α-galactosidase; SSF; Soymilk; Invertase; HPLC analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100026

Prey selectivity and functional response by larval red-eyed tetra Moenkhausia sanctaefilomenae (Steindachner, 1907) (Characiformes: Characidae)
Provedor de dados: 52
Autores: Alanis,Jorge Gallardo; Sarma,S.S.S.; Nandini,S.
Studies were carried out to quantify the functional response and prey selectivity of larval (1-5 weeks old) of Moenkhausia sanctaefilomenae, a widely cultured ornamental fish, using four rotifers (Brachionus calyciflorus, B. havanaensis, B. patulus and B. rubens) as prey. Regardless of larval age, B. havanaensis was not selected, while B. rubens and B. calyciflorus were preferred. B. patulus was selected only after three weeks. When fed B. calyciflorus, the larvae showed increased prey consumption with increasing age, but remained as plateau around 80 prey individuals. M. sanctaefilomenae consumed much lower individuals of both B. havanaensis and B. patulus, while B. rubens was consumed in higher numbers starting from the first week (about 35 ind. larva-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prey-predator interactions; Zooplankton; Fish-feeding; Brachionus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500019

Molecular Insights into the Genetic Diversity of Garcinia cambogia Germplasm Accessions
Provedor de dados: 52
Autores: Tharachand,C; Immanuel Selvaraj,C; Abraham,Z.
ABSTRACTIn this work, the genetic relationship among twelveGarcinia cambogia (Gaertn.) Desr. accessions were evaluated using Random Amplified Polymorphic DNA markers. The samples were part of the germplasm collected and maintained at NBPGR Regional station, Thrissur, India. Out of thirty RAPD primers used for screening, seven primers produced a total of 128 polymorphic markers in twelve accessions. The Polymorphic Information Content (PIC) ranged from 0.28 (OPA18) to 0.37 (OPA9) and Marker Index (MI) ranged between 3.61 (OPA12) and 5.93 (OPA3) among the primers used. Jaccard's coefficient of genetic similarity ranged between 0.07 and 0.64. The dendrogram constructed based on the similarity matrix generated from the molecular and morphological data showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Garcinia cambogia; Genetic diversity; Germplasm; Jaccard's coefficient; Malabar tamarind.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500765

Biological Synthesis of Selenium Nanoparticles and Evaluation of their Bioavailability
Provedor de dados: 52
Autores: Pouri,Saeedeh; Motamedi,Hossein; Honary,Soheyla; Kazeminezhad,Iraj.
ABSTRACT Nanoparticles due to their unique properties have attracted more attention and their bacterial biosynthesis is more favorable because is environmental friendly and the size and yield of nanoparticles can be optimized. The aim of the present study was biosynthesis of Selenium nanoparticles using Bacillus cereus. For this purpose, bacterial culture was prepared in the presence of sodium selenate solution and incubated (30°C, 24 h). The produced nanoparticles were purified through consequent centrifugation, washing with 0.9% NaCl, sonication, washing with Tris- HCl containing Sodium dodecyl sulfate (SDS) and finally isolation with water- octanol two phase systems. Then using Ultraviolet-Visible spectroscopy, dynamic light scattering (DLS), scaning...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus cereus Green chemistry Selenium; Nanoparticle.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100456

The effect of temperature and salinity on the physiological rates of the mussel Perna perna (Linnaeus 1758)
Provedor de dados: 52
Autores: Resgalla Jr.,Charrid; Brasil,Elisângela de Souza; Salomão,Luis Carlos.
The aim of this work was to study the rates of respiration, clearance, excretion and absorption efficiency at different temperature and salinity under laboratory conditions for Perna perna. Results showed variations in physiological rates and in acclimatization capacity which, taken together, enabled to understand its behavior in the environment, as well as to estimate its scope for growth. All experiments were carried out in static conditions, in ten replicas with one mussel by flasks. Perna perna was capable of achieving acclimatization for both clearance and absorption efficiency (15 to 30 ºC), but it achieved only partial acclimatization for respiration and excretion under chronic temperature conditions. The clearance and respiration rates increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mussel; Perna perna; Physiological rates; Temperature; Salinity; Net growth efficiency.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300019

Influence of different levels of aluminum on the development of citrus rootstock swingle citrumelo (Citrus paradisi mcf. x Poncirus trifoliata raf.) in nutrient solution
Provedor de dados: 52
Autores: Santos,Carlos Henrique dos; Grassi Filho,Hélio; Rodrigues,João Domingos; Pinho,Sheila Zambello de.
This work aimed at evaluating the influence of different levels of aluminum on the physiological parameters of the citrus rootstock Swingle Citrumelo in hydroponic solution. The experiment was carried out with a completely randomized design with three replications, subdivided in several lots. The levels of aluminum used were: 0, 7.5, 15, 22.5 and 30 mg L-1 in form of AlCl3.6H2O in nutrient solution. Following physiological parameters were evaluated: specific and relative foliar area, relative foliar mass, and the ratio of aerial part dry matter/root system dry matter. The results showed that aluminum did not influence the parameters evaluated except the specific foliar area, which decreased starting at the 7.5 mg L-1 level.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Rootstocks; Aluminum; Mineral nutrition; Growth analysis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100004

Current Approach in Radiochemical Quality Control of the 99mTc-Radiopharmaceuticals: a mini-review
Provedor de dados: 52
Autores: Miranda,Ana Cláudia Camargo; Durante,Ana Claudia Ranucci; Fuscaldi,Leonardo Lima; Barboza,Marycel Figols de.
Abstract To present optimized chromatographic systems for radiochemical purity (RCP) evaluation of 99mTc-eluate and 99mTc-radiopharmaceuticals, as well as to assess doses calibrator reliability for routine purposes in hospital radiopharmacies. RCP was determined by different systems and radioactivity was quantified by TLC-scanner, doses calibrator and gamma-counter. Suitable and optimized systems were presented for RCP analyses. No significant differences were observed between radioactivity counting devices and, thus, doses calibrator showed reliability for RCP determination in hospital radiopharmacies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 99mTc-radiopharmaceuticals; Radiochemical purity; Ascendant chromatography; Chromatographic system; Dose calibrator.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100903

Development of Adrenal Glands from the Embryonic Phase to Puberty in Spix’s Yellow-toothed Cavies (Galea spixii, Wagler, 1831)
Provedor de dados: 52
Autores: Lobo,Luis Miguel; Santos,Amílton Cesar dos; Oliveira,Moacir Franco de; Assis-Neto,Antônio Chaves.
ABSTRACT Several authors have underscored the importance of establishing parameters in morphological development by gender comparison to establish clinical and pre-clinical assays through the use of experimental models. Current research compares the morphometry of right and left adrenal glands of males and females and describes differentiation of the cortex and medulla tissue during the embryonic, pre-puberty and puberty phases in Spix´s yellow-toothed cavies. Embryos aged 22 (22D), 25 (25D), 30 (30D), 40 (44D) and &gt;50 (50D) days of gestation and neonates aged 15 (15DPN) (DPN= Days postnatal), 30 (30DPN) and 90 (90DPN) days after birth were analyzed. Comparisons included morphometric and histological analysis in all periods described. When compared the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal córtex; Cavidae; Experimental model; Rodentia; Steroidogenesis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100444

Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives
Provedor de dados: 52
Autores: Parada,José Luis; Caron,Carolina Ricoy; Medeiros,Adriane Bianchi P.; Soccol,Carlos Ricardo.
Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocinogenic lactic acid bacteria and/or their isolated bacteriocins are considered safe additives (GRAS), useful to control the frequent development of pathogens and spoiling microorganisms in foods and feed. The spreading of bacterial antibiotic resistance and the demand for products with fewer chemicals create the necessity of exploring new alternatives, in order to reduce the abusive use of therapeutic antibiotics. In this context, bacteriocins are indicated to prevent the growth of undesirable bacteria in a food-grade and more natural way, which is convenient for health and accepted by the community. According to their properties, structure, molecular weight...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactic acid bacteria; Bacteriocins; Purification; Food preservation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300018

Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
Provedor de dados: 52
Autores: Montañez Sáenz,Julio; Téllez,Alejandro; de la Garza,Heliodoro; de la Luz Reyes,María; Contreras-Esquivel,Juan Carlos; Aguilar,Cristóbal Noé.
Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extraction; Purification; Properties; Pectinesterase; Potato.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007

Extracts of red yolk from duck eggs inhibit metastasis of human mammary carcinoma (4T1) cells in mice
Provedor de dados: 52
Autores: Qi-bin,Song; Yi,Yao; Wei-guo,Hu; Sheng-hua,Zhang; Xiao-wei,Yan; Rui,Yang.
The aim of the present study was to investigate the in vivo anti-metastatic activity of the red pigments of red yolk eggs laid by the ducks dieting on Potamogeton cripus L on the mammary carcinoma (4T1). The pigments were extracted with petroleum ether and acetone (2:1, v/v). BALB/c mice were divided into three groups (n=6), fed with the extracts at 150 mg/kg body weight (BW)/day (DEYE-H) or at 50 mg/kg BW/day (DEYE-L) and identical buffer without the extract (control group). The extracts were administered for 34 days. The treatment significantly inhibited the growth of orthotopical 4T1 tumour (DEYE-H vs control, 1:2; DEYE-L vs control, 2:3) and reduced the metastasis of tumour in the lungs (DEYE-H vs control, 4:7; DEYE-L vs control, 5:7), without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duck egg yolk; Extracts; Mammary carcinoma; Metastasis; 4T1 Cells; BALB/c mice.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300011

Rooting of healthy and CVC-affected 'Valência' sweet orange stem cuttings, through the use of plant regulators
Provedor de dados: 52
Autores: Habermann,Gustavo; Alvarez,Rita de Cássia Félix; Modesto,Júnior Cesar; Fortes,Andréa Maria Teixeira; Rodrigues,João Domingos; Ono,Elizabeth Orika.
Citrus variegated chlorosis (CVC) is a disease caused by Xylella fastidiosa. Using different concentrations of plant regulators, such as auxins (indole-3-butyric acid) and gibberellic acid biosynthesis-inhibitor (paclobutrazol), physiological rooting capacity of healthy and CVC-affected stem cuttings were evaluated in order to investigate the importance of plant hormone imbalance and xylem occlusion in plants with CVC. The percentages of dead, alive and rooted cuttings, cuttings with callus and mean number of roots per cuttings did not show statistical differences in response to the distinct concentrations of synthetic plant regulators. There were differences only between healthy and CVC-affected cuttings. This showed the importance of xylem occlusion and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IBA; Paclobutrazol; Xylella fastidiosa; Root initiation; Citrus sinensis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100004

Chromosome Mapping and Molecular Characterization of the Tc1/Mariner Element in Rineloricaria (Siluriformes: Loricariidae)
Provedor de dados: 52
Autores: Primo,Cleberson Cezario; Glugoski,Larissa; Vicari,Marcelo Ricardo; Nogaroto,Viviane.
ABSTRACT The Tc1/Mariner sequence was isolated and mapped on chromosomes aiming to verify the association of this transposable element (TE) and chromosomal rearrangements in Rineloricaria. Cytogenetic analysis showed that Tc1/Mariner does not co-localize with chromosomal fusion points, in addition the analysis revealed intense molecular degeneration in its DNA sequence.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome rearrangement; Double-FISH; Transposon.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100310

Adsorption of glyphosate on clays and soils from Paraná State: effect of pH and competitive adsorption of phosphate
Provedor de dados: 52
Autores: Cruz,Lútecia Hiera da; Santana,Henrique de; Zaia,Cássia Thaïs Bussamra Vieira; Zaia,Dimas Augusto Morozin.
This work showed that the adsorption of glyphosate (GPS) depends on surface area for clays and amount of clays and CEC for soils. Organic matter (OM) had a secondary role in the adsorption of GPS on soils. The adsorption of GPS on soils from Londrina and Floraí counties and clays (montmorillonite, kaolinite) decreased when pH increased, however, for bentonite clay and soil from Tibagi county was kept constant. For the soils, the competitive adsorption between GPS and phosphate showed that displace of GPS by phosphate was related to the amount of clays, CEC and pH. GPS was not easily displaced by phosphate on the clays. The FT-IR spectra of the soils and clays showed that soil from Londrina resembled kaolinite. Thus, this could explain the results of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glyphosate; Adsorption; Clays; Soils.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300004

Distribution and relative abundance of anchovies (Clupeiformes-engraulididae) in the Sepetiba Bay, Rio de Janeiro, Brazil
Provedor de dados: 52
Autores: Silva,Márcio de Araújo; Araújo,Francisco Gerson de.
Distribution and relative abundance of juvenile fish of the family Engraulididae in the Sepetiba Bay, Rio de Janeiro, Brazil was assessed to detect patterns of use of the shallows during their early life cycle. Two yearly cycle (March-1996 to February-1997 and March-1997 to February-1998) were studied by a total of 120 beach net samples at five sites, two of them located in the inner Bay and three in the outer Bay near to the sea limit. Six Engraulididae species were identified in two genera: Anchoa januaria, Anchoa marinii, Anchoa tricolor, Anchoa lyolepis, Anchoviella lepidentostole and Anchoviella brevirostris, mainly juveniles in their early life cycle. A. januaria, A. brevirostris, A. lepidentostole and A. tricolor, in decreasing order, were the top...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Engraulididae; Anchoa; Anchoviella; Anchovy; Sepetiba Bay.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400005

The influence of light intensity on anatomical structure and pigment contents of Tradescantia pallida (Rose) Hunt. cv. purpurea Boom (Commelinaceae) leaves
Provedor de dados: 52
Autores: Paiva,Élder Antonio Sousa; Isaias,Rosy Mary dos Santos; Vale,Fernando Henrique Aguiar; Queiroz,Cristina Generosa de Senna.
The aim of this work was to study the effects of five different light intensities on the anatomical structure and on the pigment contents in leaves of Tradescantia pallida cv. purpurea. Once light intensity became lower, the thickness of leaf lamina and mesophyll were reduced. Adjustments in light-harvesting antenna size were observed: an increase in chlorophyll a + b/carotenoids ratio at low-light growth conditions. There was a strong positive linear correlation between the light intensity values and anthocyanin contents. Hence, T. pallida cv. purpurea acclimation to distinct environmental conditions might be related to its capacity of altering structurally and physiologically its phenotype.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf anatomy; Light intensity; Chlorophyll; Carotenoids; Anthocyanin; Tradescantia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400017

Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation
Provedor de dados: 52
Autores: Echeverrigaray,Sergio; Carrer,Rosane Postingher; Andrade,Luciana Bavaresco.
Nodal segments from greenhouse grown adult plants of Salvia guaranitica were used to evaluate the effect of culture media and growth regulators on the micropropagation and growth. The highest multiplication rate was obtained on Murashige and Skoog (MS) medium supplemented with 2.22 µM of 6-benzylaminopurine (BAP). The best condition for rooting was MS medium with 2.85 µM indole-3-acetic acid (IAA). Rooted plants were successfully acclimatized and exhibited a normal development until maturity. Using the described protocol, approximately 35 plants per explant were obtained after three months.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth regulators; Shoot culture; Axillary buds.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400018

Evidences of El Niño effects on the mullet fishery of the Patos Lagoon estuary
Provedor de dados: 52
Autores: Vieira,João Paes; Garcia,Alexandre Miranda; Grimm,Alice Marlene.
Based on biological and meteorological long-term database (1979-1983 and 1996-2000), we analyzed the variation in the juvenile recruitment and artisanal fishery landings of the mullet (Mugil platanus) in the Patos Lagoon estuary and its relationships with the regional rainfall and estuarine salinity anomalies during two strong El Niño events (1982-83 and 1997-98). Juvenile and adult mullets declined in abundance under the high rainfall and near-zero salinity that prevailed in the estuary during both El Niño events. We proposed two different hypotheses to explain the El Niño-induced effects on the juvenile and adult stages of the mullet in the estuary. First, high freshwater outflow during a very strong El Niño might render ineffective the mechanism of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artisanal fishery; ENSO; Climatic effects; Long-term studies; PELD; LTER.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200025

Genetic variability for frost resistance among Coffea accessions assessed in the field and in a cold chamber
Provedor de dados: 52
Autores: Petek,Marcos Rafael; Sera,Tumoru; Alteia,Marcos Zorzenon.
The genetic variability for frost resistance was evaluated in field conditions and cold chamber among Coffea accessions. Results showed that C. liberica var. dewevrei and C. racemosa, as well as hybrids obtained from these species were more resistant to frost. There was a great genetic variability for frost resistance among the evaluated sources (b² = 0,98). The correlation estimated between damages in the field and in the cold chamber was 0.933**, therefore, this methodology was efficient and feasible and could be potentially used in a breeding program.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee crop; Breeding; Frost resistance; Cultivar.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100003

Macauba ( Acrocomia aculeata ) pulp oil quality is negatively affected by drying fruits at 60 ºC
Provedor de dados: 52
Autores: Favaro,Simone Palma; Tapeti,Crissia Fernanda; Miranda,Cesar Heraclides Behling; Ciaconini,Gabrielly; Miyahira,Maria Amélia M; Roscoe,Renato.
ABSTRACT This study aimed to determine if the quality of macauba pulp oil is affected by drying the whole fruits at 60 ºC. Mature fruits were collected at every five days on the ground under 10 palm trees. A mixed batch of 3 kg of whole fruits, with three replications each, was dried in an oven with air circulation for 0, 12, 24, 36 and 48 h at 60 ºC. After every drying time, dried fruits were pulped using an automated device, followed by oil extraction with hexane. Moisture and oil content were determined in the pulp, and the pulp oil quality was analyzed for fatty acid composition, free fatty acids content, peroxide value, molar absorptivity at 232 and 270 nm, refractive index, and total carotene content. The tested temperature was sufficient to decrease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macauba palm; High oleic; Pulping; Pulp oil; Vegetable oil oxidation; Vegetable oil acidity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100601

Successful amplification of mitochondrial DNA from dentin of the bottlenose dolphin Tursiops truncatus
Provedor de dados: 52
Autores: Weber,Laura Isabel; Luca,Michelle Jaccottet de; Barreto,André Silva; Souza,Tiago Tolentino de.
Twenty-four teeth of Tursiops truncatus from the collection of the Marine Mammals Laboratory (FURG-RS) were analysed for their potential as DNA source for population genetic and taxonomic studies. All teeth were submitted to decontamination, grinding and demineralisation processes. Dentin powder was weighed for DNA extraction, which was evaluated by the amplification of two mitochondrial genes through PCR using universal primers. Cytochrome-b gene was amplified successfully. PCR products showed concentrations of 3-12 ng/µl for dentin powder, which was positively correlated to the quantity of dentin powder used for DNA extraction. To rule out contamination a SSCP was carried out including positive controls, and samples of humans involved in the analysis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dolphins; Tursiops; Museum specimens; Dentin; Mitochondrial DNA; Cytochrome-b.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100002

Biology and thermal requirements of Utetheisa ornatrix (L.) (Lepidoptera: Arctiidae) reared on artificial diet
Provedor de dados: 52
Autores: Signoretti,André Gustavo Corrêa; Nava,Dori Edson; Bento,José Maurício Simões; Parra,José Roberto Postali.
A study on the biology of Utetheisa ornatrix reared on the artificial diet, was conducted to determine the thermal requirements in each development stage. The aim was to find out the thermal regions in the São Paulo state where the pest could develop on Crotalaria spp. The insects were reared on an artificial diet based on the white beans and yeast. U. ornatrix thermal requirements were tested at 18, 20, 22, 25, 28, 30, and 32ºC 70±20% RH, and 14-h photophase. Lower threshold temperatures (TT) and thermal constants (K) for the egg, larval and pupal stages were 12.7ºC and 51.2 GDD, 13.5ºC and 290.9 GDD, 13.8ºC and 108.4 GDD, respectively; TT and K values for the biological cycle were 13.8ºC and 436.3 GDD.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalaria; Rattlebox moth; Rearing technique.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400001

Genes involved in brassinosteroids's metabolism and signal transduction pathways
Provedor de dados: 52
Autores: Pereira-Netto,Adaucto Bellarmino.
Brassinosteroids (BRs) are plant steroids essential for the normal growth and development, which carry an oxygen moiety at C-3 and additional ones at one or more of the C-2, C-6, C-22 and C-23 carbon atoms. In the past few years, application of molecular genetics allowed significant progress on the understanding of the BRs biosynthetic pathway regulation and on the identification of several components of their signal transduction pathway, as well. Search in eletronic databases show dozens of records for brassinosteroid-related genes for the last twelve months, demonstrating the big efforts being carried out in this field. This review highlights the recent advances on the characterization of genes and mutations that are helping to unravel the molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassinolide; Cell elongation; Dwarf; Signal perception.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400006

Determination of Beta-Lactam residues in milk by high performance liquid chromatography
Provedor de dados: 52
Autores: Brito,Roseane Brandão de; Junqueira,Roberto Gonçalves.
A high performance liquid chromatographic method to assay beta-lactam residues in milk was developed and validated. Milk samples were spiked with standard solutions and deproteinated. The extract was cleaned-up on C18 SPE cartridge, the antibiotics eluted with acetonitrile:water (50:50 v/v) and derivatized with acetic anhydride and 1-methyl-imidazole solution containing HgCl2. The chromatographic analysis was performed on C18 column using mobile phase consisting of acetonitrile and phosphate buffer (pH 6.5) in the presence of Na2S2O3 gradient and detection at 325 nm. The method was selective for ampicillin, penicillin G and penicillin V, the latter used as internal standard. Average recoveries for ampicillin and penicillin G ranged, respectively, from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics; Milk chromatography.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200007

Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus
Provedor de dados: 52
Autores: Rezende,Sebastião Tavares de; Guimarães,Valéria Monteze; Rodrigues,Marília de Castro; Felix,Carlos Roberto.
Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO). alpha-Galactosidase was induced by galactose, melibiose and raffinose, but galactose was the most efficient inducer. It was purified by gel filtration and two ion exchange chromatographies and showed Mw of 54.7 kDa. The purified enzyme showed maximal activity against p-nitrophenyl-alpha-D-galactopyranoside (pNPGal) at pH 4.5-5.5 and 55 °C, and retained about 80% of the original activity after incubation for 90 minutes at 50ºC. The KM for pNPGal was 0.3 mM. Melibiose was hydrolyzed by the enzyme but raffinose was very poor substrate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Alpha-galactosidase; Raffinose oligosaccharides.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200005

Phytase-Producing Bacteria from Extreme Regions in Indonesia
Provedor de dados: 52
Autores: Sajidan,; Wulandari,Rita; Sari,Evy Novita; Ratriyanto,Adi; Weldekiros,Hailu; Greiner,Ralf.
ABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the corresponding phytate-degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genusBacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity toB. cereus, while isolate MC8 exhibited 99% sequence identity toB. aryabhatti and D6 99% sequence identity toB. psychrotolerans. The crude extracellular phytase preparations from the isolates showed following optimal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rDNA; Bacillus sp. bacterial phytase phytate phytate-degrading enzyme.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711

Fish community composition, seasonality and abundance in Fortaleza Lagoon, cidreira
Provedor de dados: 52
Autores: Schifino,Lúcia Cabral; Fialho,Clarice Bernhardt; Verani,José Roberto.
The Fortaleza Lagoon belongs to the Southern Tramandaí subsystem, formed by lagoons disposed linearly on the north-south direction (30º 08’S, 50º 13’W). The objective of this study was to describe some aspects related to the composition of the fish community of Fortaleza Lagoon. Samples were collected monthly from November 1998 to October 1999. The specimens were captured at four previously determined points in the lagoon, and classified in five orders, 12 families and 22 species. Cyphocharax voga, Hyphessobrycon luetkenii, Oligosarcus jenynsii, Oligosarcus robustus and Loricariichthys anus were more abundant species. Amongst the sampled species only two could not be characterised as freshwater species: Centropomus sp (marine) and Lycengraulius grossidens...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fishes; Coastal lagoons; Rio Grande do Sul; Brazil.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500011

Antioxidant effect of phycocyanin on oxidative stress induced with monosodium glutamate in rats
Provedor de dados: 52
Autores: Bertolin,Telma Elita; Farias,Daniele; Guarienti,Cíntia; Petry,Fernanda Tais Souza; Colla,Luciane Maria; Costa,Jorge Alberto Vieira.
The objective of this work was to study the antioxidant effect of phycocyanin on the oxidative stress induced by monosodium glutamate in the rats. The tests were performed with 32 rats of Wistar breed, divided into four groups, which were administered saline solution of phycocyanin, monosodium glutamate and monosodium glutamate plus phycocyanin. Sulfhydryl groups and the secondary substances derived from lipid oxidation were determined through the level of TBA. The evaluation of these values and the level of sulfhydryl showed that the administration of phycocyanin presented significant antioxidant effect (p &lt; 0.05) reducing the oxidative stress induced by the monosodium glutamate in vivo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Oxidative stress; Phycocyanin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400012

Gill histopathological alterations in Nile tilapia, Oreochromis niloticus exposed to treated sewage Water
Provedor de dados: 52
Autores: Fontaínhas-Fernandes,António; Luzio,Ana; Garcia-Santos,Sofia; Carrola,João; Monteiro,Sandra.
Adult Nile tilapia, Oreochromis niloticus, of both sexes were exposed in wastewater from a sewage treatment plant for a period of 4 days. Gill samples were collected after 24, 48, 72 and 96 h and histopathological changes were analyzed by light and scanning electronic microscopy. Gill epithelium of control O. niloticus (freshwater group) was similar to that of other teleosts, while histopathological lesions were observed in exposed fishes. The main histopathological changes were edema, lifting of lamellar and filamentar epithelia and lamellar fusion. Cell proliferation with consequent thickening of the filament epithelium was also found in fishes exposed to the treated sewage water. The severity of the lesions increased with the time of exposure, namely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gill; Tilapia; Histopathology; Wastewater.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500023

Administration of venlafaxine after chronic methadone detoxification blocks post-depression relapse in rats
Provedor de dados: 52
Autores: Fadaei-Kenarsary,Meysam; Moghaddam,Hadi Fathi; Mansouri,Seyyed Mohammad Taghi; Farbood,Yaghoob.
ABSTRACT Relapse is highly prevalent after detoxification and depression. Due to the advantages of venlafaxine compared with other antidepressants, it is expected that venlafaxine administration may reduce relapse after detoxification and depression. This study aimed to evaluate the effects of venlafaxine on depression-induced relapse to morphine dependence after methadone detoxification. Eighty Sprague-Dawley rats were habituated and conditioned with morphine (10 mg/kg, S.C., for 4 days). After that, primary forced swimming and conditioned place preference (CPP) were tested. They were followed by methadone (70 mg/kg/day, P.O., for 7 days) administration, extinguishing, forced swimming stress (FSS) and administration of venlafaxine (80 mg/kg/day, I.P., for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Methadone; Depression; Relapse; Venlafaxine; Rat.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100311

Surviving probability indicators of landing juvenile magellanic penguins arriving along the southern Brazilian coast
Provedor de dados: 52
Autores: Rodrigues,Sandra Carvalho; Adornes,Andréa Corrado; Santos Filho,Euclydes Antônio dos; Silva Filho,Rodolfo Pinho; Colares,Elton Pinto.
The aim of this work was to monitor and study the hematocrit and weight of juvenile penguins, with and without oil cover, found alive along the southern coast of Brazil, after capture, as well as before eventual death or release. Released juvenile penguins showed higher weight and hematocrit (3.65 ± 0.06 kg and 44.63 ± 0.29%, respectively) than those that died (2.88 ± 0.08 kg and 34.42 ± 1.70%, respectively). Penguins with higher hematocrit and weight after capture had higher mean weight gain than their counterparts with smaller hematocrit and weight after the capture. Besides, juveniles with higher hematocrit and weight after the capture had higher survival rates, independent of the presence or absence of oil. The results suggested that juveniles covered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disease; Survival; Oil; Hematocrit; Body weight; Spheniscus magellanicus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200021

Genome-wide Analysis of LBD (LATERAL ORGAN BOUNDARIES Domain) Gene Family in Brassica rapa
Provedor de dados: 52
Autores: Huang,Xiaoyun; Liu,Gang; Zhang,Weiwei.
ABSTRACT LOB (lateral organ boundaries)-domain proteins deﬁne a family of plant-speciﬁc transcription factors involved in developmental process from embryogenesis to seed production. They play a crucial role in shaping the plant architecture through coordinating cell fate at meristem to organ boundaries. Identification of LBD genes from Brassica rapa genome, and analysis of phylogeny，gene structure, chromosome location, phylogenetic and tissue expression pattern analysis of LBD family genes in Chinese cabbage will be useful to the functions identification of plant LBD genes. Based on Brassica rapa genome database and bioinformatic method, Chinese cabbage LBD family genes were identified and the genes were sequenced. A phylogenetic tree was created using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica rapa; LBD; Phylogenetic analysis; Gene expression; Genome-wide.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100439

Reducing COD level on oily effluent by utilizing biosurfactant-producing bacteria
Provedor de dados: 52
Autores: Jacobucci,Daniela Franco Carvalho; Oriani,Maria Raquel de Godoy; Durrant,Lucia Regina.
Two bacteria isolated from crude oil contaminated soil, Pantoea agglomerans and Planococcus citreus, produced biosurfactants utilizing 1.5% of kerosene and olive oil as the sole carbon sources, respectively. The bacteria and the biosurfactants produced were introduced to oily effluent, arising from margarine and soap industry. Emulsification activities were determined by increases in the absorbance of the oil-in-water emulsions at 610 nm, whereas the water-in-oil emulsions were expressed as the height (cm) of the emulsion layers formed. The 72 h incubation experiment resulted in a COD (Chemical Oxygen Demand) reduction of 76% with Planococcus citreus strain and 70% with Pantoea agglomerans.The COD reduction with bacterial biosurfactants was over 50% in 24...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactants; Bacteria; COD; Oily effluent; Bioremediation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400029

Population structure, condition factor and reproductive period of Astyanax paranae (Eigenmann, 1914) (Osteichthyes: Characidae) in a small and old Brazilian Reservoir
Provedor de dados: 52
Autores: Abelha,Milza Celi Fedatto; Goulart,Erivelto.
The population structure, condition and reproductive period of Astyanax paranae adult individuals inhabiting a small reservoir were investigated. The fishes were quarterly captured from the riverine and lacustrine zones of the Alagados Reservoir, Paraná, Brazil, during 1996/1997 and 1998/1999. The sex ratio differed from 1:1 with the predominance of the females in all standard length classes and total population sample. The females showed higher condition than the males. Both the sexes presented lower mean condition values in April and higher mean condition values in July. The estimated values of the regression coefficient indicated isometric growth for the females and males. The gonadosomatic relationship reached a maximum value of 14.80% for the females...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Condition factor; Freshwater fish; Gonadosomatic relationship; Sexual proportion.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300009

Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion
Provedor de dados: 52
Autores: Diniz,Leandro Eugênio Cardamoni; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Torres,Fabrício Medeiros; Ruas,Eduardo Augusto; Santos,Melissa de Oliveira; Sera,Tumoru; Ruas,Paulo Maurício.
The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Genetic variability; RAPD; Restriction endonucleases.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002

Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze)
Provedor de dados: 52
Autores: Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Vidor,Mário; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria angustifolia; Conifer; Somatic embryogenesis; Picea abies; Embryo maturation; Pinus sp.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015

Nitrification in hybrid reactor with a recycled plastic support material
Provedor de dados: 52
Autores: Wolff,Delmira Beatriz; Ochoa,Juan Carlos; Paul,Etienne; Costa,Rejane Helena Ribeiro da.
This work investigated the nitrification in a hybrid moving bed pilot scale reactor, which used a low density recycled plastic support material for biomass growth. The filling rate was 20% of its working volume (22L). The feeding and recirculation outflow was 45L/day. The reactor operated at a temperature of 16ºC, in two phases, according to the sludge retention time (SRT): A phase was 10 days and B phase was 3 days (average values). The applied average volumetric nitrogen and organic loads were 0.16KgTKN/m3.day and 1kgCOD/m³.day, respectively. The results showed an average nitrogen removal of 95% and average COD removal of 89%, in both A and B phases. Nitrogen removal rates were independents of the SRT.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hybrid reactor; Nitrification; Support; Wastewater treatment.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400030

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa
Provedor de dados: 52
Autores: Mascarin,Gabriel Moura; Alves,Sérgio Batista; Lopes,Rogério Biaggioni.
This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was evaluated in solid grain substrates. In solid-state fermentation, the best conidia production was achieved with the soybean meal and broken corn for I. farinosa, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biphasic fermentation; Entomopathogenic fungi; Isaria fumosorosea; Isaria farinosa; Agro-industrial products.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400002

Alcoholic fermentation by the wild yeasts under thermal, osmotic and ethanol stress
Provedor de dados: 52
Autores: Silva,Rosimeire Oenning da; Batistote,Margareth; Cereda,Marney Pascoli.
This study aimed to explore the variability in the metabolism of nine wild yeasts isolated from the sugarcane juice from a distillery in the Brazilian State of Mato Grosso. Cell viability under the stress conditions was evaluated. The yeasts were inoculated in the test tubes containing sugarcane juice adjusted from 12 to 21º Brix, ethanol from 6 to 12% in volume and temperature at 30, 35 and 40ºC. The viability was established by the growth in Petri dishes and visually by the CO2 production in the test tubes. None of the evaluated yeasts showed simultaneous resistance to the three stress conditions. The potential of yeast BB.09 could be emphasized due to its ability to ferment up to12% ethanol at 30°C.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bio prospection; Alcohol distillery; Cellular viability; Fermentation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200001

Composition and seasonal variation of the ichthyofauna from upper Rio Paraguaçu (Chapada Diamantina, Bahia, Brazil)
Provedor de dados: 52
Autores: Santos,Alexandre Clistenes de Alcântara; Caramaschi,Érica Pellegrini.
The aim of this work was to characterize the composition of the ichthyofauna of the upper course of the Rio Paraguaçu. Two excursions were carried out, one in the dry season, and the other in the rainy season, with 36 localities sampled. A total of 63 species were collected. Multivariate analyses indicated that abiotic variables such as the channel order and altitude were the main influences on the number of species present. The number of species in each excursion was equivalent in the two seasons, suggesting that seasonal changes have little influence on the ichthyofauna of this area.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rio Paraguaçu; Chapada Diamantina; Fish community; Abiotic parameters; Seasonality.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400012

Mapping and Characterization of the Grid-connected Photovoltaic Systems in the City of Curitiba: Preliminary Results
Provedor de dados: 52
Autores: Scolari,Bruno Sabino; Tonolo,Édwin Augusto; Pan,Roberto Chun Yan; Urbanetz Júnior,Jair.
ABSTRACT This work presents the mapping of grid-connected photovoltaic systems supported by the Normative Resolution (NR) of the National Agency of Electric Energy (ANEEL) Nº 482/2012 in the city of Curitiba. Firstly, a brief introduction was made justifying the reasons that led to the development of this study, followed by the explanation about the methodology, procedures and criteria adopted in the study. A general mapping of all on-grid photovoltaic systems supported by NR Nº 482/2012 installed in the city of Curitiba, identified by class, is presented, indicating how the urban distribution of these photovoltaic systems occurs, and it is possible to observe in which regions the highest prevalence of installations occurs. From this, the general...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic Solar Energy; Grid connected photovoltaic systems; Normative Resolution ANEEL Nº 482/2012.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200230

Morphological and histochemical analysis of the antennal gland of Macrobrachium Potiuna (Muller, 1880) (Crustácea, Decapoda, Palaemonidae)
Provedor de dados: 52
Autores: Moraes,Neci; Scaff,Rejane Cirra.
The objective of our study was to describe by histologic methods the structural organization of the antennal gland of M. potiuna as well as the location through histochemical methods of the neutral and sulfated polissacharides that exist in these regions. This study showed that the gland is composed of the a non-secretive tubular portion and a secretive portion which is formed by a cubic simple epithelium, sub-epithelium layer and cellular chains
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antennal gland; Macrobrachium potiuna; Morphology; Histochemistry.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300005

Radiopharmaceuticals for sentinel node detection
Provedor de dados: 52
Autores: Frier,Malcolm.
Imaging of sentinel nodes is a valuable technique in the management of some types of cancer. Several different radiopharmaceuticals have been successfully employed in the technique, but there have been few systematic studies to examine the relationship between physicochemical properties of the radiopharmaceuticals used and in-vivo performance. The present study describes the preparation and characterisation of radiocolloids of similar chemical composition but differing in particle size distribution in an attempt to identify the role of variations in the single parameter of size.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sentinel node; Radiocolloid; Particle size.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500011

Application of plant growth regulators at pre-harvest for fruit development of 'PÊRA' oranges
Provedor de dados: 52
Autores: Almeida,Isolina Maria Leite de; Rodrigues,João Domingos; Ono,Elizabeth Orika.
The aim of the present work was to evaluate the effects of auxins and gibberellins when applied at pre-harvest to the fruit development, and to the ripening and natural fall of the fruit, in 'Pêra' oranges. Trees of Citrus sinensis Osbeck cv. Pêra, 5 years old, were utilized. The treatments applied were: GA3 + 2,4-D 12.5mg L-1 of each; GA3 + 2,4-D 25mg L-1 ; GA3 + 2,4-D 37.5mg L-1; GA3 + NAA 12.5mg L-1; GA3 + NAA 25mg L-1; GA3 + NAA 37.5mg L-1; NAA + 2,4-D 12.5mg L-1; NAA + 2,4-D 25mg L-1; NAA + 2,4-D 37.5mg L-1; and water (control). The treatments were applied 3 times, at intervals of 45 days. The variables evaluated were: rate of natural fall (%), fruit length and diameter (mm), and fresh fruit weight (g). None of the treatments promoved alterations in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auxins; Gibberellins; Fruit drop; Development.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400003

Drying and rehydration of oyster mushroom
Provedor de dados: 52
Autores: Apati,Giannini Pasiznick; Furlan,Sandra Aparecida; Laurindo,João Borges.
Dehydration and rehydration processes of Pleurotus ostreatus fruiting bodies were investigated in this work. Mushroom samples were dehydrated at 40, 50 and 60 ºC, using drying air with relative humidity of 75 %. The rehydration was investigated at different temperatures of immersion water (25, 55 and 85 ºC) and different immersion times (30, 75 and 120 minutes). The best rehydration occurred for the samples dried at 40 ºC. The rehydration could be done in water at room temperature, during 30 minutes. Water sorption isotherms of samples were determined at 30, 40 and 50 ºC. Both GAB and BET models satisfactorily represented the experimental data of moisture sorption of dried mushrooms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pleurotus ostreatus; Dehydration; Rehydration; Moisture sorption isotherms.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400025

Spatial heterogeneity and its influence on Copaifera langsdorffii Desf. (Caesalpiniaceae)
Provedor de dados: 52
Autores: Resende,Júlio Carlos França; Klink,Carlos Augusto; Schiavini,Ivan.
Population dynamics and structure of young individuals of the tree Copaifera langsdorffii Desf. (Caesalpiniaceae) were studied in the gallery forest of the Panga Ecological Station, Uberlândia, MG, Brazil. Three distinct habitats were recognized in this forest, Dike, Middle and Edge zone. Four annual surveys of plant height, diameter at plant base and density were made between 1993 and 1996. The species showed an aggregated distribution in all three habitats. Population structure in all four surveys presented the typical reversed "J" form, characteristic of populations in local regeneration. For the whole population, mortality rates during the four years were constant and low (average of 4.8 % per year), while recruitment declined during the same period...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallery forest; Seedling; Recruitment; Mortality; Growth; Saplings.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300012

Morfologia dos tricomas em Tibouchina sect. Pleroma (D. Don) cogn. (melastomataceae)
Provedor de dados: 52
Autores: Guimarães,Paulo José Fernandes; Ranga,Neusa Taroda; Martins,Angela Borges.
Melastomataceae é a família que apresenta a maior diversidade de tricomas. Dr. Wurdack em seu "Atlas of hairs" reconheceu 46 tipos de tricomas para as Melastomataceae neotropicais com o uso de MEV, e referiu 17 destes para o gênero Tibouchina. Em Melastomataceae a morfologia dos tricomas tem sido extensivamente usada na delimitação dos táxons, assim como em estudos que antecederam ao trabalho de Cogniaux, a monografia das Melastomataceae brasileiras publicada em 1885. Em Tibouchina sect. Pleroma a morfologia dos tricomas foi substancial para a circunscrição de várias espécies. O uso da MEV permitiu uma caracterização dos 15 tipos diferentes de tricomas observados nas 41 espécies examinadas. Os diferentes tipos são descritos com base no aspecto morfológico...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichome; Morphology; Melastomataceae; Tibouchina.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400015

Bioethanol production from sweet potato (Ipomoea batatas L.) flour using co-culture of Trichoderma sp. and Saccharomyces cerevisiae in solid-state fermentation
Provedor de dados: 52
Autores: Swain,Manas Ranjan; Mishra,Jyoti; Thatoi,Hrudayanath.
The aim of this work was to study the optimiation of co-culturing of Trichoderma sp. and Saccharomyces cerevisiae (1:4 ratio) on sweet potato (Ipomoea batatas L.) flour (SPF) for the production of bio-ethanol in solid-state fermentation (SSF). Maximum ethanol (172 g/kg substrate) was produced in a medium containing 80% moisture, ammonium sulphate 0.2%, pH 5.0, inoculuted with 10% inoculum size and fermented at 30ºC for 72h. .Concomitant with highest ethanol concentration, maximum ethanol productivity (2.8 g/kg substrate/h), microbial biomass (23×10(8) CFU/ g substrate), ethanol yield (47 g/100g sugar consumed) and fermentation efficiency (72%) were also obtained under these conditions. Cell interaction was observed familiar between the viable cells of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma sp.; Saccharomyces cerevisiae; Sweet potato flour; Solid state fermentation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200002

Encapsulation of lycopene using spray-drying and molecular inclusion processes
Provedor de dados: 52
Autores: Nunes,Itaciara Larroza; Mercadante,Adriana Zerlotti.
This study aimed to obtain encapsulated lycopene in a powder form, using either spray-drying or molecular inclusion with beta -cyclodextrin ( beta -CD) followed by freeze-drying. The encapsulation efficiency using spray-drying ranged from 94 to 96%, with an average yield of 51%, with microcapsules showing superficial indentations and lack of cracks and breakages. Lycopene- beta -CD complexes were only formed at a molar ratio of 1:4, and irregular structures of different sizes that eventually formed aggregates, similar to those of beta -CD, were observed after freeze-drying. About 50% of the initial lycopene did not form complexes with beta -CD. Lycopene purity increased from 96.4 to 98.1% after spray-drying, whereas lycopene purity decreased from 97.7 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopene; Encapsulation; Spray-drying; Gum arabic; Inclusion complex; Beta -cyclodextrin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500018

Thermodynamics of molecular recognitions between antineoplastic drug taxol and phosphatidylcholine
Provedor de dados: 52
Autores: Süleymanoglu,Erhan.
The aim of this study was to study the basic features of Taxol recognition with phospholipids by applying the thermodynamic and spectroscopic measurements. The obtained information could be used further for deductions on its precise cellular and pharmacological mechanisms of action, on improvements of its solubility properties by phospholipids, as well as for designing the novel lipidic carriers for drug delivery.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antineoplastic agents; Chemotherapy; Taxol; Phoshatidylcholine; Taxol-membrane interactions; Membrane fluidity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600011

Partial resistance to fruit necrosis associated to Colletotrichum spp. among arabic coffee genotypes
Provedor de dados: 52
Autores: Sera,Gustavo Hiroshi; Sera,Tumoru; Ito,Dhalton Shiguer; Azevedo,José Alves de; Ribeiro Filho,Claudionor; Mata,João Siqueira da.
The aim of this study was to evaluate the resistance to fruit necrosis associated to Colletotrichum spp. in Coffea arabica L.. Twenty-two arabic coffee genotypes were performed in a randomized block design, with three replications and ten plants per plot. They were evaluated for the variables fruit necrosis, luminosity index inside of the plant canopy and fruits per productive nodes of the plants. There was genetic variability for the resistance to fruit necrosis. Different partial resistance levels, varying from highly susceptible (score = 3.89) to moderately resistant (score = 1.77) were observed. The genotypes that presented moderate resistance were the cultivars IPR-100, IPR-103, IPR-105 and IPR-108 and the coffee selection IAPAR-00023.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antracnosis; Coffea arabica; Coffee breeding; Colletotrichum gloeosporioides; Cultivars.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300005

Structure, composition and spatial distribution of tree species in a remnant of the semideciduous seasonal Alluvial Forest of the upper Paraná River Floodplain
Provedor de dados: 52
Autores: Campos,João Batista; Romagnolo,Mariza Barion; Souza,Maria Conceição de.
The aim of this investigation was to study the structure, distribution patterns, and dynamics of tree species in a remnant of the Semideciduous Seasonal Alluvial Forest in the Upper Paraná River Floodplain, Brazil. Spatial variation of the vegetation was considered mainly in relation to soil and topographic characteristics. The 5,946 m² study area extended along a transect from the bank of the Paraná River across the floodplain, passing through a lake. Phytosociological analysis were performed for 36 plots, each 150 m². Subanalysis were performed for 300 m² zones. Soil characteristics and topographic profile and their relationship to the vegetation were analyzed. Hydrodynamic and sediment processes were the most important factors in vegetation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alluvial forest; Floristic distribution; Hidrodynamic; Phytosociology; Riparian forest.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200008

Stage preference and functional response of Rhynocoris longifrons (Stål) (Hemiptera: Reduviidae) on three hemipteran cotton pests
Provedor de dados: 52
Autores: Sahayaraj,Kitherian; Kalidas,Subramanian; Tomson,Majesh.
In this work, the stage preference and functional response of the indigenous reduviid bug Rhynocoris longifrons feeding on five different densities of the cotton aphid Aphis gossypii, Phenacoccus solenopsis, and Dysdercus cingulatus was examined in Petri dish arenas containing cotton leaves under laboratory conditions. The reduviid predator exhibited a Type II functional response at all hemipteran pests evaluated when data were fit to Holling's disc equation. Predatory rate gradually increased while the predator grew older and adults consumed maximum number of D. cingulatus and P. solenopsis. An opposite trend was observed, while the reduviid was provided with Aphis gossypii. The rate of attack on P. solenopsis was quite low but fairly consistent, with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotton pests; Functional response; Reduviid predator; Stage preference.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500013

Investigation of the GJB6 Deletion Mutations Del (GJB6-D13s1830) and Del (GJB6-D13s1854) in Iranian Patients with Autosomal-Recessive Non-Syndromic Hearing Loss (ARNSHL)
Provedor de dados: 52
Autores: Onsori,Habib.
Hearing loss (HL) is the most common inherited sensory disorder affecting about 1 in 1000 births. The first locus for nonsyndromic autosomal recessive HL is on chromosome 13q11-22. The two genes, GJB2 and GJB6, are closely located on chromosome and are known to be co-expressed in the embryonic cochlea. Deletion mutations involving GJB6 were associated with autosomal-recessive nonsyndromic hearing loss (ARNSHL) and in combination with a GJB2 mutation with digenic ARNSHL. The objective of this study was to screen for the del (GJB6-D13S1830) and del (GJB6-D13s1854) mutations in GJB6 gene in patients with ARNSHL from Iran, using multiplex PCR and direct sequencing methods. Agarose gel electrophoresis and DNA sequencing of amplified fragment of the PCR reaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hearing loss; GJB6; GJB2; Deletion Mutation; Iran.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100311

Monitoring and spatial distribution of heterotrophic bacteria and fecal coliforms in the Rodrigo de Freitas Lagoon, Rio de Janeiro, Brazil
Provedor de dados: 52
Autores: Lutterbach,Márcia T. S.; Vazquez,Joselito Cabral; Pinet,Jorge A.; Andreata,José V.; Silva,Alexandre Carlos da.
The distribution of heterothrophic bacteria and fecal coliforms was monitored at four sampling stations located near the shore of the Rodrigo de Freitas Lagoon, in the city of Rio de Janeiro, Brazil. Water samples were collected, monthly from October 1994 through September 1998. The highest heterothrophic count (6.5x10 7 CFU/100mL) was recorded at stations 2 and 4 during August 1998 and the lowest (10 ³ CFU/100 mL) at station 3 during February 1995. With respect to fecal coliforms, the highest and lowest counts were 1.6x10 5 coliforms/100mL at station 3 during March 1997 and &lt;1 coliform/100mL at all the stations during February 1995 and September 1997 as well as station 3 during February 1998. The data indicated a percentage increase of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fecal coliforms; Heterotrophic bacteria; Rodrigo de Freitas Lagoon; Monitoring.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100002

Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content
Provedor de dados: 52
Autores: Sorgatto,Vanessa Ghiggi; Carvalho,Julio Cesar de; Sydney,Eduardo Bittencourt; Medeiros,Adriane Bianchi Pedroni; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
Abstract We present an improved method of direct transesteriﬁcation suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial oil; Ethanolysis; FAEE; Microscale; Screening.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401

Preparation and evaluation of modified composition for lyophilized kits of [Cu(MIBI)4]BF4 for [99mTc] technetium labeling
Provedor de dados: 52
Autores: Lima,Marisa de Jesus de C.; Marques,Fabio Luiz Navarro; Okamoto,Miriam Roseli Yoshie; Garcez,Alexandre Tales; Sapienza,Marcelo Tatit; Buchpiguel,Carlos Alberto.
The [Cu(MIBI)4]BF4 complex was synthesized and different formulations for lyophilized kits that could be cost-effectively used with different routines in nuclear medicine laboratories were investigated. In one preparation the kit components were kept similar to the Cardiolite® , except that the SnCl2.2H2O concentration was increased to 0.150 mg. In a second formulation, component concentrations were reduced to 1/5 of the original value and the SnCl2.2H2O concentration was adjusted to 0.04 mg. These products were labeled with maximum activities of 55.5 GBq and 8.14 GBq, respectively, and have shown an average radiochemical purity of 95 %. Biodistribution of the products was assessed by dissection in mice and in rabbits, and did not show any statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: [99mTc]technetium; [[99mTc](MIBI)6]+; Radiopharmaceutical; Biodistribution.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700001

Editorial
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600001

A Simple and High-yield Synthesis of Hexadecyl Ferulate and Its In Vitro Antioxidant Potential
Provedor de dados: 52
Autores: Nadal,Jessica; Pedroso,Flávia de B.; Minozzo,Bruno Rodrigo; Brito,Priscilla Salles de; Farago,Paulo Vitor; Vellosa,José Carlos Rebuglio; Miyoshi,Edmar.
ABSTRACT Ferulic acid (FA) is a phenolic compound with well-known antioxidant potential that can be used as a promising anti-inflammatory and anti-cancer molecule. Furthermore, it has been reported to have neuroprotective activity. One of the main problems, which limit its clinical use, is its low bioavailability when administered orally. This limitation can be circumvented by changes in their structure and/or for preparing lipid-based formulations. The aim of this study was to synthesize a derivative of FA, the hexadecyl ferulate (HF). This compound would be more susceptible to pass through blood-brain barrier (BBB) due to its lipophilic character. The HF was obtained by Steglich esterification and yielded 76.77 ± 1.35%. Its structural characterization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABTS•+ assay; DPPH• assay; Ferulic acid derivative; Scavenger activity; Steglich esterification.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100428

Physicochemical Characteristics of Oil Obtained from the Treatment of Poultry Abattoir Effluents and its Potential for Biodiesel Production
Provedor de dados: 52
Autores: Kunh,Sheila Simone; Mantovan,Fabiana de Marqui; Milinsk,Maria Cristina; Alves,Helton José.
ABSTRACT Sludge oil derived from the effluent generated in poultry abattoirs offers a promising biomass for the production of biodiesel. In this work, determination was made of its physicochemical characteristics, including acidity, density at 20ºC, peroxide index, moisture content, and saponification index, together with evaluation of a route for its conversion to biodiesel.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oil sludge; Biomass; Biodiesel; Renewable energy sources; Abattoir effluents.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200212

Anatomia do caule de Lycopersicon Esculentum Mill., submetido a diferentes tratamentos com reguladores vegetais
Provedor de dados: 52
Autores: Martins,Maria Bernadete Gonçalves; Castro,Paulo Roberto de Camargo e.
The effects of plant growth regulators GA3 50 mg. L-1, NAA 100 mg. L-1, CCC 1500 mg.L-1 and SADH 3000 mg.L-1 on stem anatomy of Lycopersicon esculentum Mill cv. Ângela Gigante were studied. Two sets of experiments were carried out in greenhouse during two separte periods. Anatomical studies , revealed that growth promotors induced increased xylem thickness and increased the number of tracheary elements while the growth retardants decreased xylem thickness and induced fiber formation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant growth regulators; Anatomy; Stem; Lycopersicon esculentum.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200014

Dinâmica do chumbo no lago do parque Ingá, Maringá, PR, Brasil
Provedor de dados: 52
Autores: Vaz,Sérgio Renato; Lenzi,Ervim; Luchese,Eduardo Bernardi; Fávero,Luzia O. B.
Este trabalho analisa a dinâmica do chumbo no lago do parque do Ingá, Maringá - PR. Mensalmente durante um ano foram coletadas amostras de água e sedimentos e o pH e temperatura medidos no ato da coleta. Nas amostras de água foi determinada a Demanda Química de Oxigênio (DQO) e a concentração total de Pb na água e sedimentos. A determinação da concentração Pb na água e sedimentos foi feita com a técnica da espectrometria da absorção atômica, modalidade chama. Os valores médios mensais encontrados, em intervalos e médias globais foram respectivamente: concentração de Pb na água, em ng.mL-1, não detectado (nd) - 55,56 e 19,23; Pb em sedimentos, em µg.g-1, 48,73 - 92,87 e 71,93; pH, 7,02 - 8,23 e 7,60; temperatura, em °C, 19,33 - 27,48 e 23,73; DQO, em...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lead; Lakes; Environmental pollution; Elements environmental dynamic; Environment.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400010

Distribution of Cu, Fe, Mn, and Zn in Two Mangroves of Southern Brazil
Provedor de dados: 52
Autores: Madi,Ana Paula Lang Martins; Boeger,Maria Regina Torres; Reissmann,Carlos Bruno.
ABSTRACT This study assessed the relation between Cu, Fe, Mn, and Zn in the soils of the mangroves of Antonina and Guaratuba, on the coastline of the State of Paraná, and in the leaf tissues of A. shauerianaR. mangle, and L. racemosa through the analysis of correlation levels between these two compartments. Leaf samples were collected for ten individuals of each species in a 1000 m2 area delimited in each mangrove. Soil samples from 0-10 cm depth were taken from under the crown projection area of the selected trees to be submitted to chemical analysis. In the soils, metallic micronutrients presented the following order: Fe &gt; Mn &gt; Zn &gt; Cu. In the leaves, concentrations were species dependent. InA. shaueriana and R. mangle, the profile was Mn &gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avicennia schaueriana; Laguncularia racemosa; Rhizophora mangle; Mangrove; Micronutrients.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600970

Use of fibres obtained from the cashew (Anacardium ocidentale, L) and guava (Psidium guayava) fruits for enrichment of food products
Provedor de dados: 52
Autores: Matias,Maria de Fátima O.; Oliveira,Edson Leandro de; Gertrudes,Eduardo; Magalhães,Margarida Maria dos Anjos.
With the purpose of using the cashew and guava dried bagasse for enrichment of cookies, a study of drying process was done. The physico- chemical characterization included the determination of pH, soluble solids, total acidity, protein, lipids, fiber, ash, moisture and total and reducing sugars. Total coli forms, yeasts and molds counting were the microbiological analyses conducted. Enriched cookies were prepared by adding 5, 10 and 15% (of total weight) of dry residues of cashew and guava. Appearance, color, odor, taste and texture were the sensorial attributes evaluated for the enriched and non-enriched cookies. The granulometric analysis defined that more adequate particle to add were between 65 and 100 mesh. The enriched cookies with cashew and guava...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fiber; Fruit bagasse; Guava; Cashew.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400018

Maize-Dwelling insects omnivory in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) egg masses
Provedor de dados: 52
Autores: Menezes-Netto,Alexandre Carlos; Varella,Andrea Corrêa; Fernandes,Odair Aparecido.
This work reports the first record of omnivory behavior of Diabrotica speciosa (Coleoptera, Chrysomelidae), Leptoglossus zonatus (Hemiptera, Coreidae), Monocrepidius aff. posticus and Monocrepidius fuscofasciatus (Coleoptera, Elateridae) on fall armyworm Spodoptera frugiperda (Lepidoptera, Noctuidae) egg masses in maize fields. Macrophotography was used to record the activity of these insects on fall armyworm sentinel egg masses during 2009 and 2010 maize growing seasons. The presence of omnivorous insects changes the species population dynamics within the ecosystem. Therefore, the implications of these interactions should be understood and taken into consideration for integrated pest management enhancement.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Facultative predator; Phytozoophagy; Predation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100012

Genetic relationships among strains of the Aspergillus niger aggregate
Provedor de dados: 52
Autores: Ferracin,Lara Munique; Frisvad,Jens Christian; Taniwaki,Marta Hiromi; Iamanaka,Beatriz Thie; Sartori,Daniele; Schapovaloff,Maria Elena; Fungaro,Maria Helena Pelegrinelli.
We analyzed the genetic relationships between 51 fungal isolates previously identified as A. niger aggregate, obtained from dried fruit samples from worldwide origin and 7 A. tubingensis obtained from Brazilian coffee beans samples. Greater fungal diversity was found in black sultanas. Aspergillus niger sensu stricto was the most prevalent species. It was found in all fruit substrates of all geographical origins. Based on Random Amplification of Polymorphic DNA (RAPD) and β-tubulin sequences data two groups of A. niger were found. In spite of the small number of isolates from Group IV an association between extrolite patterns and molecular clustering is speculated. A. tubingensis were the second most frequent species and this species were clearly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger aggregate; Ochratoxin A; Dried fruits; Toxigenic fungi.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700031

Effect of Partial and Total Replacement of Inorganic by Organic Microminerals Sources on the Quality of Broiler Carcasses
Provedor de dados: 52
Autores: Lopes,Marcos; Paroul,Natalia; Barbosa,Juliana; Valduga,Eunice; Cansian,Rogerio Luis; Toniazzo,Geciane; Oliveira,Débora.
ABSTRACT Among the nutrients involved in the chicken diet, the microminerals deserve attention as they exert essential functions in the organism. These compounds can be provided in inorganic (traditional) and organic (chelate) forms. In organic form, the micromimerals can attend a new concept related to a better bio-availability. In this sense, the objective of this work was to evaluate the effect of the partial and total substitution of inorganic by organic sources of microminerals on the animal diet, assessing its concentration in the blood and liver after 21 and 40 days and also in the bones after 40 days. Moreover, the effect on the physical-chemical and sensory characteristics of the muscle after 15 days of storage at 4°C was also evaluated. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bio-availability; Broilers; Microminerals; Nutrition.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100440

Enhancing Antitumor Efficacy of Cisplatin Low Dose by EDTA in Ehrlich Ascetic Carcinoma Bearing Mice
Provedor de dados: 52
Autores: El-Naggar,Sabry Ali; El-Said,Karim Samy; Mobasher,Maysa; Elbakry,Mohamed.
Abstract In a recent study, the treatment of different human cancer cell lines in vitro with ethylene diamine tetra-acetic acid (EDTA) showed a promising anticancer activity which could be a novel promising approach for cancer treatment. The aim of this study is to address the ability of EDTA to enhance the antitumor efficacy of the low dose of cisplatin (Cis) treatment in Ehrlich ascetic carcinoma (EAC) bearing mice. Sixty female albino mice were divided into six groups. The 1st group of mice was served as a negative control. 2nd - 6th groups were inoculated intraperitoneal (i.p) with 2×106 EAC cells/mouse. After one day of inoculation, the 2nd, 3rd and 4th groups were injected daily for 6 days (early treatment) with phosphate buffer saline, low dose of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Antitumor; Ethylene diamine tetra-acetic acid (EDTA); Ehrlich ascites carcinoma.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100316

Interference of a commercial catalase preparation in laccase and peroxidase activities
Provedor de dados: 52
Autores: Ballaminut,Nara; Yamanaka,Renata; Machado,Katia Maria Gomes.
The influence of commercial catalase preparations (fungal and bovine origin) on laccase and peroxidase activity assays was evaluated using enzymatic extracts obtained from several basidiomycetes grown under different culture conditions. No hydrogen peroxide was detected in the extracts. Inhibition of laccase activity by 40 to 80% was related to the catalase source. In addition, oxidation of the substrate (ABTS) by fungal catalase in the absence of the enzymatic extract from basidiomycetes was observed. The results demonstrated the need for the evaluation of interference of the commercial catalase preparation when its use was required in the reaction mixture.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxidase; Fungi; Basidiomycetes; Hydrogen peroxide; Enzymatic inhibition.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500017

Evaluation of the Antidiabetic and Antibacterial Activity of Cissus sicyoides
Provedor de dados: 52
Autores: Beltrame,Flavio Luis; Pessini,Greisiele Lorena; Doro,Dani Luce; Dias Filho,Benedito Prado; Bazotte,Roberto Barbosa; Cortez,Diógenes Aparício Garcia.
In this work we investigated the antidiabetic and antibacterial effect of Cissus sicyoides (CS) from Brazil. Diabetic rats that received water (A group) or extracts from the aerial parts of the plant (Cs group) during four weeks were employed. After this period, serum levels of glucose, cholesterol and triglycerides were measured. Glycemia was not affected by treatment with CS. However, there was an increased cholesterol and triglyceride level in Cs group. In addition, bioassay-guided fractionation of methanolic extract from aerial parts of CS was performed for isolation of antibacterial compounds.beta-Sitosterol and sitosterol-beta-D-glucopyranoside isolated showed antibacterial activity against Bacillus subtilis with minimal inhibitory concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cissus sicyoides; Vitaceae; Experimental diabetes mellitus; Antibiosis; Steroids.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100004

Mayfly diversity in the brazilian tropical headwaters of Serra do Cipó
Provedor de dados: 52
Autores: Goulart,Michael; Callisto,Marcos.
The objectives of this study were to evaluate spatial and seasonal distribution, assemblage structure and substrate associations of mayfly nymphs in five lotic ecosystems in the headwaters of the Doce River and São Francisco River watersheds, Brazil. Samples were taken in 24 sampling stations during rainy and dry seasons in 1998. In total, 7,066 organisms were collected, belonging to 27 genera and 6 families of Ephemeroptera. The dominant taxa were Americabaetis, Paracloeodes, Leptohyphes and Hagenulus. During the rainy period, the São Francisco River watershed showed higher taxonomic richness, diversity and evenness, while in the dry period, the taxonomic richness was higher in Doce River watershed. No significant differences were found in the taxonomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ephemeroptera; Habitat diversity; Substrate association; Conservation; River ecology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800015

Use of secondary sewage water as a culture medium for Chaetoceros gracilis and Thalassiosira Sp (Chrysophyceae) in laboratory conditions
Provedor de dados: 52
Autores: Costa,Rauquírio André Albuquerque Marinho da; Koening,Maria Luise; Macêdo,Sílvio José de.
Experiments were carried out in order to test the efficiency of additions of secondary sewage as a culture medium for Chaetoceros gracilis and Thalassiosira sp (Chrysophyceae) under laboratory conditions. These algae were cultivated in sea water with concentrations of 10%, 20%, 30% and 40% of wastewater. The results were compared with those obtained by the nutritive medium f2 of Guillard (1975). The best results in terms of cellular densities were observed at 40% additions. There were significant differences (significance levels of 5%) between the nutritive medium f2 and the 40% additions for both the species. Maximum cellular densities observed for all additions tested were, 4,125.00 x 10³ cells/ml for Chaetoceros gracilis on the ninth day and 834.00 x...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalgae (Chrysophyceae); Culture; Secondary sewage.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200011

Extracellular enzymes of Fusarium graminearum isolates
Provedor de dados: 52
Autores: Kikot,Gisele Eleonora; Hours,Roque Alberto; Alconada,Teresa María.
Fusarium graminearum isolates from three different agroecological regions in Argentina were examined according to the production of different extracellular enzyme activities of potential biotechnological interest: pectinases (PGase: polygalacturonase and PMGase: polymethylgalacturonase), cellulase (CMCase: carboxymethylcellulase) and hemicellulase (xylanase). The isolates were grown in minimum salt medium supplemented with 0.25% glucose, 0.125% citric pectin and 0.125% oat bran as carbon sources and/or enzyme inducers. PGase activity was detected early (after two days of incubation) in all the cultures; it was found to be the highest for all the isolates. PMGase was high only for those isolates of the II region. CMCase and endoxylanase activities were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular enzymes; Fusarium graminearum; Pectinases; Cellulases; Hemicellulases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400005

Aptamer-based radiopharmaceuticals for diagnostic imaging and targeted radiotherapy of epithelial tumors
Provedor de dados: 52
Autores: Missailidis,Sotiris; Perkins,Alan; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
In the continuous search for earlier diagnosis and improved therapeutic modalities against cancer, based on our constantly increasing knowledge of cancer biology, aptamers hold the promise to expand on current antibody success, but overcoming some of the problems faced with antibodies as therapeutic or delivery agents in cancer. However, as the first aptamer reached the market as an inhibitor against angiogenesis for the treatment of macular degeneration, aptamers have found only limited applications or interest in oncology, and even less as radiopharmaceuticals for diagnostic imaging and targeted radiotherapy of tumours. Yet, the chemistry for the labelling of aptamers and the options to alter their pharmacokinetic properties, to make them suitable for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aptamer; Radiopharmaceutical; Diagnostic imaging; Radiotherapy; Epithelial tumor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700013

Spatial variability of the physical and mineralogical properties of the soil from the areas with variation in landscape shapes
Provedor de dados: 52
Autores: Souza,Zigomar Menezes de; Marques Júnior,José; Pereira,Gener Tadeu.
The objective of this work it was to use the geostatistics methods to investigate the spatial relationships between the physical and mineralogical properties of an oxisol planted with the sugarcane in an area of slight variations in the landform. The soil was sampled at 10 m regular intervals in the crossing points of a 100 x 100 m grid. At each point, the soil was collected at 0.0-0.2 m, 0.2-0.4 m and 0.4-0.6 m depths for the analyzes of physical properties and at 0.6-0.8 m for the mineralogical analyses. Both the Kt/Kt+Gb ratio and Kt relative crystallization level were higher in the compartment I than in the compartment II. As a consequence, the soil penetration resistance and bulk density were higher in the compartment I, while the macroporosity and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Geostatistics; Soil-landscape relationship.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200007

The genoprotective activity of resveratrol on permethrin-induced genotoxic damage in cultured human lymphocytes
Provedor de dados: 52
Autores: Turkez,Hasan; Aydin,Elanur.
The aim of this work was to investigate the genetic effects of resveratrol (RSV) at concentrations of 10, 15, 25, 40, 75 and 100 µM and its activities on the genotoxicity induced by the permethrin (PM) (200 µM). After the application of PM and RSV, separately and together, cultured human lymphocytes were assessed by chromosome aberrations (CA) and sister chromatid exchange (SCE) tests. According to results, the frequencies of CA and SCE rates in the peripheral lymphocytes were significantly increased by PM compared with the controls. However, RSV had no genotoxic effect. Furthermore, the findings revealed that PM-induced increases in the mean frequencies of both genotoxic indices were diminished by RSV in a clear dose dependent manner, indicating its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome aberration; Mutagenicity; Permethrin; Resveratrol; Sister chromatid exchange.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300008

Capture of Anastrepha sororcula (Diptera: Tephritidae) in McPhail and Jackson traps with food attractant and virgin adults
Provedor de dados: 52
Autores: Felix,Christiane dos Santos; Uchôa-Fernandes,Manoel Araécio; Faccenda,Odival.
This stady evaluated the capture of A. sororcula in the traps baited with the conspecific virgin adults and food attractant in two orchards. The first was the orchard of the Universidade Federal da Grande Dourados (OUFGD) and the second, the orchard of the Sindicato Rural de Dourados (OSRD). The capture of A. sororcula in McPhail and Jackson traps was carried out using the corn hydrolysed protein (CHP), control (no flies), virgin males (5, 10 and 15), five virgin females and five virgin couples. The average number of the flies caught in the traps with the corn hydrolysed protein was signifícantly higher than all the other treatments. There was no significant capture of A. sororcula females in the traps baited with the conspecific virgin males, females or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sexual attraction; Fruit fly; Guava Orchard.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100013

An evaluation of antibacterial activities of Psidium guajava (L.)
Provedor de dados: 52
Autores: Sanches,Neviton Rogério; Garcia Cortez,Diógenes Aparício; Schiavini,Michelle Simone; Nakamura,Celso Vataru; Dias Filho,Benedito Prado.
The present study was designated to evaluate the antibacterial activities of aqueous and ethanol:water extracts from leaves, roots and stem bark of Psidium guajava L. The antibacterial activities of the extracts against bacteria were tested by using both microdilution assay. The aqueous extracts of P. guajava leaves, roots and stem bark were active against the gram-positive bacteria Staphylococcus aureus (MICs=500, 125 and 250 µg/ml, respectively) and Bacillus subtilis (MICs=500 µg/ml), and virtually inactive against the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (MICs &gt;1000 µg/ml). The ethanol:water extracts showed higher antimicrobial activity as compared to aqueous extracts. Based on this finding, the ethanol:water extract of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psidium guajava; Antibacterial activities; Flavonoids; Alpha- and beta-amyrin; Beta-sitosterol.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300014

Dry matter production and distribution in three cassava (Manihot esculenta Crantz) cultivars during the second vegetative plant cycle
Provedor de dados: 52
Autores: Sagrilo,Edvaldo; Vidigal Filho,Pedro Soares; Pequeno,Manoel Genildo; Gonçalves-Vidigal,Maria Celeste; Kvitschal,Marcus Vinícius.
A study was carried out in Araruna County, State of Paraná, to understand the relationship between the total dry matter yield and its proportion allocated to the storage roots of cassava (Manihot esculenta Crantz) plants in the second vegetative cycle. The experimental design was a randomized complete block in split-plot scheme with four replications. The plots consisted of the Mico, IAC 13 and IAC 14 cultivars and the monthly harvesting dates were assessed in the sub-plots. The results showed that the Mico and IAC 13 cultivars were more efficient in allocating dry matter to the storage roots. The IAC 14 cultivar allocated a higher proportion of assimilates to stems compared with the other two cultivars. With regard to the influence of harvesting time, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Harvesting time; Dry matter allocation; Harvest index.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600001

Chemical profile, agaritine and selenium content of Agaricus bisporus
Provedor de dados: 52
Autores: Mohamed,Eman Mostafa.
Chemical profile of the Agaricus bisporus samples were analyzed using GC/MS method in ethanolic extracts. A total of 174 metabolic products were detected, which included 13 significant metabolites between 1.2 to 83 % (w/w); other 13 metabolites at 1% (w/w) and 148 metabolites less than 1% classified into 12 categories. These metabolites had many medicinal activities, which included anti-cancer, anti-cardiovascular diseases, anti-hypercholesterol, anti-microbial, hepatoprotective, human health supporting and immune enhancer. HPLC analysis of water extracts of the A. bisporus samples showed that the spores and gills in the fresh adult mushroom had higher percentage of agaritine mycotoxin than the other parts. These contents were decreased by different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus bisporus; Chemical profile; Agaritine; Selenium; Anticancer; Antihypercholesterol; Aroma compounds; Galvanometric; GC/MS and HPLC.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600015

Land Use and Environmental Zoning of Mixed Ombrophilous Forests for Sustainable Use (Irati National Forest, Brazil Southern Region)
Provedor de dados: 52
Autores: Mazza,Carlos Alberto da Silva; Mazza,Maria Cristina Medeiros; Almeida,Dayana; Santos,José Eduardo dos; Fushita,Angela Terumi.
ABSTRACT An environmental zoning proposal due to the demand to develop strategies to ensure biodiversity scenarios for the remaining mixed ombrophilous forests was carried out for the Irati National Forest, a legally protected area for sustainable use located in southern Brazil. To identify land use and cover, Ikonos PSM images dated from December 2004, based on the interpretation of visual character and vectorization of orbital data were used. Qualitative and quantitative classifications identified six types of land and cover use: native forest, forestry, capoeira grass, wetland, araucaria plantation, and anthropic. The native forest relative to the mixed ombrophilous forest was the prevailing type of land cover. The environmental zoning proposal defined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental zoning; Environmental characterization; Biodiversity conservation; Legally protected area; Geographic information system.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100703

Physico-chemical characterization of Ilex paraguariensis St. Hil. during the maturation
Provedor de dados: 52
Autores: Zaions,Ivanor; Picolo,Ana Paula; Gonçalves,Itamar Luís; Borges,Ana Claudia Piovezan; Valduga,Alice Teresa.
In Brazil, yerba mate is consumed after processing; however, in Chile and Uruguay, the consumers prefer the cured product, which acquires a yellow color. For that yerba-mate is stored for a period of six months to one year, which increases the cost of the final product for the overseas market. This study evaluated the effect of humidity and temperature in maturation chamber on the time required for the product to get this characteristic. The changes in the color, pH, moisture and water activity were evaluated during the time of storage in different conditions of temperature and humidity. Yerba-mate subjected to higher temperature and humidity showed nearest color of the product submitted to natural storage. The loss of green color was related to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba-mate; Early maturation; Color.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500663

Ocean Dwelling Actinobacteria as Source of Antitumor Compounds
Provedor de dados: 52
Autores: Gomathi,Ajitha; Gothandam,K M.
ABSTRACT Extending over decades, research has been of great focus and enormous progress on exploring the ocean for natural products from marine actinobacteria. Attraction towards alternative medicine has led to improvements in natural product discovery. With great potential to survive in extreme environments, marine actinobacteria, efficiently produce an array of metabolites with diverse bioactivity by evolving the secondary metabolic pathways. Exploiting the secondary metabolite producing potential of actinobacteria, many compounds with antitumor, antibacterial, antifungal, antimalarial, antiprotozoal, antiparasitic, antiviral, anti-parasitic, anti-inflammatory activities has been discovered. Efforts in bioprospecting alternative sources of natural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine actinobacteria; Bioactivity; Antitumor compounds.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100343

Biotransformation of the monoterpene, limonene, by Fusarium verticilloides
Provedor de dados: 52
Autores: Oliveira,Brás Heleno de; Strapasson,Rogério Antônio.
Limonene, the main constituent of the orange peel oil, was used as substrate for the biotransformation by the fungus Fusarium verticilloides. A hydroxylated derivative of limonene was isolated from the culture broth and characterized by spectroscopic methods. It was identified as perillyl alcohol, an important anti-cancer compound.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Limonene; Perillyl alcohol; Biotransformation; Fusarium verticilloides.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100002

Diet of Lontra longicaudis (Olfers, 1818) (Carnivora: Mustelidae) in three limnic systems in Southern Rio Grande do Sul State, Brazil
Provedor de dados: 52
Autores: Quintela,Fernando Marques; Artioli,Luiz Guilherme Schultz; Porciuncula,Rafael Almeida.
The aim of the present work was to study the diet of Lontra longicaudis in three limnic systems (anthropogenic shallow lakes, pluvial channel and coastal stream) in Rio Grande do Sul State coastal plain, southern Brazil. Fishes were the most consumed item in all the three systems, being Mugilidae the most representative family in the pluvial channel and coastal stream and Cichlidae in the shallow lakes. Other identified items were mollusks, insects, crustaceans, amphibians, reptiles, birds and mammals and vegetal fragments. The high frequency of birds in the shallow lakes was remarkable, considering the lower frequencies of this item in previous investigations on the species diet. There was a high frequency of swamp eels (Synbranchidae, Synbranchus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthropogenic shallow lakes; Pluvial channel; Coastal stream; Neotropical otter.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600011

Organic acid effect on calcium uptake by the wheat roots
Provedor de dados: 52
Autores: Nunes,Fabrize Caroline; Miyazawa,Mário; Pavan,Marcos Antonio.
This work evaluated the effect of the natural organic acids on the uptake of Ca by the wheat roots in a hydroponic solution. The following organic acids were evaluated: citric, oxalic, tartaric, malic, malonic, maleic, DL-malic, p-hydroxybenzoic, aconitic, and salicilic. The organic acids neither enhanced the root growth nor increased Ca uptake. The salicilic and malic acids were highly toxic and decreased the root growth. The citric, tartaric, maleic, aconitic, and salicilic decreased the Ca uptake by the roots due to their higher capacity to form the stable complexes with Ca in solution at pH 6.0. Decreasing the Ca valence from Ca++ to CaL+ or CaL2(0) through the organic ligand complexation reactions decreased the Ca uptake. The results suggested that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrient uptake; Metal-organic complex; Organic ligand; Organic molecule.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100002

Evaluation of the extraction process of the essential oil from Cymbopogon schoenanthus with pressurized carbon dioxide
Provedor de dados: 52
Autores: Sousa,Elisa Maria Bittencourt Dutra de; Câmara,Ana Paula Costa; Costa,Wallace Albuquerque; Costa,Adriano Cezar J.; Oliveira,Humberto Neves Maia; Galvão,Elisângela Lopes; Marques,Márcia Maria Ortiz.
The objective of this work was to study the essential oil extraction process of Cymbopogon schoenanthus Spreg with pressured CO2, and to compare the results with a conventional extraction process. The experiment was conducted at 15°C, 66.7 bar and a medium flow rate of 1.23 g CO2/min, in a fixed bed extractor and a dynamic method was adopted for the solubility calculation. The solvent extraction was realized with ethanol in an incubator, followed by a vacuum filtration and solvent evaporation. The composition of the oil was analyzed by a gas chromatography connected to a mass spectrometer. The extraction with CO2 showed a yield of 2.78%, and solubility of 1.48 x 10-2 g oil/ g CO2.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extraction/carbon dioxide; Cymbopogon schoenantus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400028

Purification and characterisation of trypsin-like enzyme from the pyloric caeca of cod (Gadus morhua) II
Provedor de dados: 52
Autores: Beirão,Luiz Henrique; Mackie,Ian Mckintoch; Teixeira,Evanilda; Damian,César.
A trypsin -like enzyme from the pyloric caeca of cod (Gadus morhua) was purified by affinity chromatography on CHOM Sepharose 4B. Some characteristics were established by its catalytic activity on T.A.M.E., typical enzyme substrate, and serine protease inhibitors. The enzyme had an isoelectric point of 5.30 and 5.89 and was very similar in amino acid composition to bovine trypsin, but differed in having a higher relative amount of acidic amino acids and a lower amount of basic amino acids. The enzyme also hydrolysed fish protein substrates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cod; Enzyme; Trypsin.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100005

Optimization of Malaxation Process using Major Aroma Compounds in Virgin Olive Oil
Provedor de dados: 52
Autores: Cevik,Serife; Ozkan,Gulcan; Kıralan,Mustafa.
ABSTRACT Optimization of major aroma compounds in olive oils produced from fruits at three maturity stages wasstudied. A central composite design was used for the optimization of malaxation conditions of temperature and times, each at five levels with 13 runs including five central points. The responses of interest were trans-2-hexenal and hexanal, which were investigated and their contents were optimized. A full quadratic second order regression model including the linear, quadratic, and two factor interaction effects was proposed to explain the variation in the contents of target compounds depending on the malaxation conditions. Adequacies of models were evaluated by checking regression coefficients for each model. Models were found to work with high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hexanal; Trans-2-hexenal; Maturity; Response surface method (RSM).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100516

Essential oils of camphor tree (cinnamomum camphora nees &amp;amp; eberm) cultivated in Southern Brazil
Provedor de dados: 52
Autores: Frizzo,Caren D.; Santos,Ana C.; Paroul,Natalia; Serafini,Luciana A.; Dellacassa,Eduardo; Lorenzo,Daniel; Moyna,Patrick.
The essential oils of two varieties of Camphor tree (Cinnamomum camphora Nees &amp;amp; Eberm, Lauraceae), known as Hon-Sho and Ho-Sho cultivated in experimental stands in Southern Brazil were studied. The essential oils were obtained from the leaves and twigs of young plants by hydrodistillation. The identification of the components was performed using GC, GC/MS and retention indexes on methyl silicone and carbowax phases. The main components identified were linalool in the Ho-Sho and camphor in the Hon-Sho.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cinnamomum camphora; Leaves; Essential oils; GC/MS; Linalool; Camphor.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300011

The effect of losartan on angiotensin II-induced cell proliferation in a rat aorta smooth muscle cell line
Provedor de dados: 52
Autores: Tambelline,Natália; Oliveira,Karen; Olchanheski Junior,Luiz Renato; Sordi,Regina; Otuki,Michel Fleith; Favero,Giovani Marino; Fernandes,Daniel.
The success of revascularization procedures is limited by recurrent stenosis, which is a narrowing of a blood vessels that results from neo-intimal hyperplasia. The renin-angiotensin-aldosterone system has been implicated in the pathogenesis of neo-intimal hyperplasia, and a role for angiotensin II in vascular smooth muscle cell proliferation has been proposed. There are at least two high-affinity subtypes of angiotensin II receptors, AT1 and AT2, both of which are seven-transmembrane G protein-coupled receptors. We investigated the effect of losartan, an AT1 receptor antagonist, on vascular smooth muscle cell proliferation using the A7r5 smooth cell line derived from rat aorta. Losartan was shown to prevent angiotensin II-induced cell proliferation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Losartan; Cell proliferation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200012

Morphological and molecular identification of four Brazilian commercial isolates of Pleurotus spp. and cultivation on corncob
Provedor de dados: 52
Autores: Menolli Junior,Nelson; Asai,Tatiane; Capelari,Marina; Paccola-Meirelles,Luzia Doretto.
The species of Pleurotus have great commercial importance and adaptability for growth and fructification within a wide variety of agro-industrial lignocellulosic wastes. In this study, two substrates prepared from ground corncobs supplemented with rice bran and charcoal were tested for mycelium growth kinetics in test tubes and for the cultivation of four Pleurotus commercial isolates in polypropylene bags. The identification of the isolates was based on the morphology of the basidiomata obtained and on sequencing of the LSU rDNA gene. Three isolates were identified as P. ostreatus, and one was identified as P. djamor. All isolates had better in-depth mycelium development in the charcoal-supplemented substrate. In the cultivation experiment, the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Charcoal; Edible mushroom cultivation; Molecular analysis; Taxonomy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200019

Effects of Allelochemicals from Ficus microcarpaon Chlorella pyrenoidosa
Provedor de dados: 52
Autores: Jiang,Zhongyang; Peiyong,Guo; Chang,Cheinchi; Gao,Lili; Li,Shixiong; Wan,Jinjin.
This study was performed in order to isolate and identify unknown allelochemicals from Ficus microcarpa, and to investigate the inhibitory to bloom-forming of green alga Chlorella pyrenoidosa. Through gradient elution, fraction C2, whose inhibition of alga growth in diverse extracts was the strongest was shown to cause significant reductions of maximum quantum yield, as well as electron transport rates of C. pyrenoidosa. The study data also showed that the increase of fraction C2 concentration decreased the activity of total superoxide dismutase (SOD), but increased the activities of catalase (CAT) and malondialdehyde (MDA) content. These results demonstrate that the active fraction C2 not only induced the photoinhibition or photodamage of PSII reaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allelochemical; Phenols; Photosynthesis; Ficus microcarpa; Chlorella pyrenoidosa.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400595

Harnessing Natural Colorants for Sustainable Textile Dyeing an Eco-Friendly Approach Using Sweet Cane (Saccharum Bengalense Retz.) Inflorescence
Provedor de dados: 52
Autores: Raza,Ali; Iqbal,Naeem; Mahmood,Saqib; Parveen,Shagufta; Azeem,Muhammad; Nawaz,Muhammad; Javed,M Tariq; Noman,Ali.
ABSTRACT The excessive industrial use of dye producing plants has threatened the existence of these species and biodiversity. Exploration of abundantly available natural dye sources not only provide industrial benefits but also share the load of dye yielding plants. In the present study we used the inflorescence of munj sweet cane (Saccharum bengalense Retz.) to extract natural colorant for textile industry. This easily available plant is cheap source of natural colorants and good alternative of synthetic dyes. The munj sweet cane inflorescence extract and fabric was treated with microwave radiations. The dyeing aspects like temperature, time, fabrics to extract ratio, salt type and salt concentrations were optimized. Pre-and post mordanting was carried...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irradiation; Natural dye; Munj sweetcane; Color fastness; Mordanting; Spectraflash.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100702

Necessary auxiliary background for efficient use of an existing computer program of non-parametric fitting of nonlinear equations
Provedor de dados: 52
Autores: Papamichael,Emmanuel M.; Theodorou,Leonidas G.
This work constituted a significant contribution for more efficient use of a valuable computer program of non-parametric fitting of nonlinear multiparametric equations to experimental data. However, prerequisite in this context was the transformation of nonlinear multiparametric equations into linear hyperplane forms before their incorporation within the computer program; this latter was decisive and a matter of proper programming practice. Herein, a series of widely used equations useful in different fields of chemical processes, in biochemistry and/or in biotechnology, along with their suitable transformations as well as the appropriate programming support are being reported.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-parametric methods; Computer program; Fitting of nonlinear equations.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200022

Performance of pilot-scale vertical flow constructed wetlands with and without the emergent macrophyte Spartina alterniflora treating mariculture effluent
Provedor de dados: 52
Autores: Sousa,Wilson Treger Zydowicz; Panitz,Clarice Maria Neves; Thomaz,Sidinei Magela.
Vertical flow constructed wetlands, planted with and without Spartina alterniflora, were tested for the treatment of mariculture wastewater. Wetlands with and without the emergent macrophyte produced reductions of 89 and 71% for inorganic solids, 82 and 96% for organic solids, 51 and 63% for total nitrogen, 82 and 92% for ammoniacal nitrogen, 64 and 59% for orthophosphate, and 81 and 89% for turbidity, respectively. Wetlands with S. alterniflora showed denitrification tendencies, while wetlands without S. alterniflora had higher oxygen levels leading to nitrification. The results suggest the fundamental role of oxygen controlling the purification processes as well as the potential of constructed wetlands to treat mariculture effluents.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial wetlands; Dissolved oxygen; Nitrification; Denitrification; Pollutant removal; Mariculture effluent.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200024

Cellular aging: theories and technological influence
Provedor de dados: 52
Autores: Mercado-Sáenz,Silvia; Ruiz-Gómez,Miguel J.; Morales-Moreno,Francisco; Martínez-Morillo,Manuel.
The aim of this article was to review the factors that influence the aging, relationship of aging with the biological rhythms and new technologies as well as the main theories to explain the aging, and to analysis the causes of aging. The theories to explain the aging could be put into two groups: those based on a program that controlled the regression of the organism and those that postulated that the deterioration was due to mutations. It was concluded that aging was a multifactorial process. Genetic factors indicated the maximum longevity of the individual and environmental factors responsible for the real longevity of the individual. It would be necessary to guarantee from early age the conservation of a natural life rhythm.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Longevity; Biological rhythms; Caloric restriction; Telomerase; Free radical.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600008

Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR
Provedor de dados: 52
Autores: Ma,Min Min; Jiang,Dan; Li,Shuang Ya; Wu,Yuan; Meng,Ju; Huang,Jiang Yao.
ABSTRACT Adulterant herbal materials are threats to import and export trade and consumer safety. In this study, we established a simple and rapid examination system for the detection of Phellodendron chinense Schneid. Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and loop-mediated isothermal amplification (LAMP), were developed for traditional Chinese medicine detection, and their specificity and sensitivity were compared. The DNA of P. chinense was extracted and its special periods amplified with designed primers. Real-time PCR and LAMP experiments were conducted to test the specificity of primers in contrast to other similar species. The template concentration was diluted from 101 ng/µL to 10-5 ng/µL in order to contrast...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Traditional Chinese medicine; Real-time PCR; Phellodendron chinense Schneid; LAMP; Specificity; Sensitivity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100314

Start-up and steady-state conditions of an Anaerobic Hybrid Reactor (AHR) using mini-filters composed with two types of support medium operating under low loading rates
Provedor de dados: 52
Autores: Silva,Vivian Galdino da; Campos,Cláudio Milton Montenegro; Pereira,Erlon Lopes; Silva,Júlia Ferreira da.
The present work aimed at studying the operational parameters of an Anaerobic Hybrid Reactor (AHR) removing organic matter of coffee wastewater with low concentration. The AHR was built similar to an UASB reactor, however the interior was filled with mini-filters composed by two types of support materials: expanded clay and rolled pebble. Three start-ups were accomplished in order to achieve the stationary state (steady-state). Three hydraulic retention times were appraised: 28.5; 24.0 and 18.0 h, obtaining a volumetric loading rate (VLR) of 0.70; 0.56; 0.54 kg COD m-3 d-1 and a biological organic loading rate (BOLR) of 0.0156; 0.0103 and 0.01213 kg BOD5 kg TVS-1 d-1. Due to the decrease in the concentration of organic matter in the influent, the endogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental chemistry; Coffee wastewater; Biodegradation; Optimization process.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500015

Optimized Exon-Exon Junction Library and its Application on Rodents' Brain Transcriptome Analysis
Provedor de dados: 52
Autores: Dou,Tong-Hai; Gao,Yuan; Chen,Cheng-Wen; Xu,Min-Jie; Fu,Mao-Bin; Zhang,Liang; Zhou,Yan.
ABSTRACT  Background: Alternative splicing (AS), which plays an important role in gene expression and functional regulation, has been analyzed on genome-scale by various bioinformatic approaches based on RNA-seq data. Compared with the huge number of studies on mouse, the AS researches approaching the rat, whose genome is intermedia between mouse and human, were still limited. To enrich the knowledge on AS events in rodents' brain, we perfomed a comprehensive analysis on four transcriptome libraries (mouse cerebrum, mouse cerebellum, rat cerebrum, and rat cerebellum), recruiting high-throughput sequencing technology. An optimized exon-exon junction library approach was introduced to adapt the longer RNA-seq reads and to improve mapping efficiency....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA-seq; Exon-exon junction; Alternative splicing; Cerebrum; Cerebellum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100400

In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil
Provedor de dados: 52
Autores: Droste,Annette; Silva,Anelise Machado da; Matos,Adriana Vieira; Almeida,Júlia Winck de.
Micropropagation studies were carried out using the seeds for establishing an in vitro culture of Vriesea gigantea and Vriesea philippocoburgii. Germination rate of V. gigantea was higher than of V. philippocoburgii. Plantlets of V. philippocoburgii gave rise to many adventitious shoots when cultivated in Knudson basal medium. In contrast, for V. gigantea, a higher salts-concentration was needed, so that the number of shoots was increased by Murashige and Skoog medium. Addition of activated charcoal and naphthaleneacetic acid in regeneration medium allowed the growth of shoots and the formation of roots, confirming the success of in vitro culture. The differences in expression of the genotypes reinforce the need of more research in order to set up the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Conservation; Propagation; Bromeliaceae; Vriesea.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600006

Nematicidal activity of Paecilomyces marquandii proteases on infective larvae of Ancylostoma spp
Provedor de dados: 52
Autores: Genier,Hugo Leonardo André; Queiroz,José Humberto de; Braga,Fabio Ribeiro; Soares,Filippe Elias de Freitas; Araújo,Jackson Victor de; Pinheiro,Iara Rebouças.
ABSTRACT The present study aimed to evaluate the action of Paecilomyces marquandii proteases on Ancylostoma spp L3. White halos in the zymogram confirmed the proteolytic action. Difference (p &lt;0.01) between the number of L3 in the differents groups was found, with 41.4% of reduction of Ancylostoma spp. L3 before 24 hours.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Nematophagous fungi; Protease; Zoonosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100423

Impact of the Icaraí Sewage Outfall in Guanabara Bay, Brazil
Provedor de dados: 52
Autores: Marques Júnior,Aguinaldo Nepomuceno; Crapez,Mirian Araujo Carlos; Barboza,Conceição Denise Nunes.
The present study aimed to evaluate the water quality of the Icaraí Sewage Outfall (ISEO) area. Sampling was conducted during winter and summer, and water samples were analyzed for dissolved oxygen, pH, temperature, salinity, dissolved inorganic nutrients (DIN), seston, particulate organic matter (POM), and pigments. Results showed that the water chemistry of the area suffers temporal and spatial variation. Great variability was also seen in the C:N:P ratios of POM (August, 112:30:1; December, 59:11:1) and in the DIN concentrations. Chlorophyll-a and ammonium concentrations (4.5 µg L-1 - 71.2 µg L-1, and 0.20 µM - 52,6 µM, respectively) characterized the ISEO area as eutrophic. The dispersal of the material was not effective under certain oceanographic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eutrophication; Sewage outfall; Water pollution; Nutrients; Guanabara Bay.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500014

Cadmium and Chromium Toxicity to Pseudokirchneriella subcapitata and Microcystis aeruginosa
Provedor de dados: 52
Autores: Rodgher,Suzelei; Espíndola,Evaldo Luiz Gaeta; Simões,Fernanda Cristina Fonseca; Tonietto,Alessandra Emanuele.
The toxicity of cadmium and chromium to Pseudokirchneriella subcapitata and Microcystis aeruginosa was evaluated through algal growth rate during 96h exposure bioassays. Free metal ion concentrations were obtained using MINEQL+ 4.61 and used for IC50 determination. Metal accumulations by the microorganisms were determined and they were found to be dependent on the concentration of Cd2+ and Cr6+. IC50 for P. subcapitata were 0.60 µmol L-1 free Cd2+ and 20 µmol L-1 free Cr6+, while the IC50 values for M. aeruginosa were 0.01 µmol L-1 Cd2+ and 11.07 µmol L-1 Cr6+ . P. subcapitata accumulated higher metal concentrations (0.001 -0.05 µmol Cd mg-1 dry wt. and 0.001 -0.04 µmol Cr mg-1 dry wt) than the cyanobacteria (0.001 -0.01 µmol Cd mg-1 dry wt and 0.001 -0.02...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxic effects; Algae sensitivity; Metal accumulation; IC50.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100020

Measurement of the gassing power of bakers' yeast: correlation between the dough volume and the incubation time
Provedor de dados: 52
Autores: Borzani,Walter.
An empirical equation is proposed to correlate the dough volume and the incubation time during cylinder tests using thin flour dough carried out to evaluate the gassing power of compressed yeast. The above equation permitted to correlate the gassing power of the yeast and the proof time, as well as to calculate the specific rate of the dough volume variation at any time. It provided more information regarding the fermentation power of the yeast than the sole value of its gas-producing power. A physical interpretation of the proposed equation is presented.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bakers' yeast; Gassing power; Fermenting power; Gas-producing power.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200008

Seasonal fluctuation of some limnological variables on a floodplain lake (Patos lagoon) of the Upper Paraná River, Mato Grosso do Sul State, Brazil
Provedor de dados: 52
Autores: Rodrigues,Luzia Cleide; Train,Sueli; Roberto,Maria do Carmo; Pagioro,Thomaz Aurélio.
Temporal variations of some limnological variables in limnetic and littoral regions of Patos lagoon, a "várzea" lake on the floodplain of the Upper Paraná River (22(0) 43'12"S e 53(0) 17'37"W), were studied in relation to hydrological and climatological factors. Measurements of water temperature, euphotic zone, suspended material, electrical conductivity, total alkalinity, pH, dissolved oxygen, carbon dioxide, total nitrogen, total Kjeldahl nitrogen, total phosphorus, dissolved phosphorus, reactive soluble phosphorus and chlorophyll a were taken monthly between March 1993 and February 1994. Results lend evidence to the importance of the hydrological regime on the dynamic of limnological variables in floodplain lakes. A dilution of nutrients in the lagoon,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Limnological variables; Flood pulse; Floodplain; Paraná River; Connectivity; Nutrients.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600014

Chemical composition of Ilex paraguariensis St. Hil. under different management conditions in seven localities of Paraná State
Provedor de dados: 52
Autores: Reissmann,Carlos Bruno; Radomski,Maria Izabel; Quadros,Ruth Maria Bianchini de.
Ilex paraguariensis St. Hil. (erva-mate), the mate tea, is a forest tree whose natural distribution in the Brazilian territory embraces the States of Mato Grosso do Sul, Paraná, Santa Catarina and Rio Grande do Sul. Its economical importance is moreover related to tea production. The purpose of this study is to characterize the leaf chemical composition in relation to different plantation and management conditions. Chemical analysis were performed on plants of different ages,in naturally occurring areas, planted stands, and seedbeds. The most remarkable elements observed in the study were P with average values ranging from 0,5 - 3,2 g kg-1 D.W., Mn values from 346 - 3.330 mg kg-1 D.W., and Al values from 167 - 1.235 mg kg-1 D.W.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macronutrients; Micronutrients; Aluminium; Foliar analysis; Ilex paraguariensis; Erva-mate.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200009

Partial Purification and Characterization of β-glucosidase fromMonascus sanguineus
Provedor de dados: 52
Autores: Dikshit,Rashmi; Tallapragada,Padmavathi.
The aim of the present work was to study the production and characterization of β-glucosidase from Monascus sanguineus. Agro-waste residues were screened to obtain the maximum yield of enzyme. Jack fruit seed was the best substrate for enzyme production. Studies on the optimization of pH and temperature showed acidic pH favorable for enzymatic activity, whereas the optimum temperature was 60°C. Enzyme kinetics studies with different concentration of pNPG showed the calculated value of Km approximately 0.89 mM with the non-linear regression and 0.98 mM with the linear regression techniques. The enzyme was predominantly inhibited by KCl (69.8%) and moderately inhibited by CaCl2(14.8%). Studies on the sensitivity for glucose showed that after 100 mM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymography; Agro-waste; Kinetics; Monascus sanguineus; PNPG.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200185

Correlation between K, Mn, Fe, Cu and Zn in natural honeys from Eucalyptus sources
Provedor de dados: 52
Autores: Mattos,Silvânia V. M.; Bastos,Esther M.; Dayrell,Ilda O.; Nelson,David L.
Thirty-five natural honey samples from three apicultural regions of the state of Minas Gerais, Brazil, were collected from honeybee hives under various climatic conditions over a two-year period. The beehives were located in the districts of Bom Jesus do Amparo, Barão de Cocais and São Gonçalo do Rio Abaixo, all within a 100 km radius of Belo Horizonte, Minas Gerais, Brazil. The pollinic spectrum, color and ash, moisture, K, Fe, Mn, Cu and Zn contents were determined. Elemental analysis were done by flame atomic absorption spectrometry. Accuracy and precision were verified by recovery tests and relative standard deviation, respectively. The mean mineral contents encountered were K = 1130; Mn = 3.88; Fe = 2.79; Zn = 2.34 and Cu = 0.54 µ g/g. During the dry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Honey; Honey mineral content; Pollinic analysis; Eucalyptus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200001

The effect of melatonin and/or complex vitamin B1,B6,B12 in modulating epinephrine-induced stress in male rats
Provedor de dados: 52
Autores: EL-Toweissy,Mona Yossef; Mohamed,Nema Abdel-Hameed; Abdel-Wahab,Wessam Mohamed.
This study was undertaken to determine the modulating effects of intramuscular administration of melatonin (MT)(1mg/ kg) and/or Tri-B (B 1 , B 6 and B 12 ) (20mg/kg) on body weight and some biochemical changes in rats induced by Epinephrine (Epi) injection. The data showed that MT and/or Tri-B treatment effectively improved the changes in malondialdehyde, lipid profile, blood sugar level and insulin. MT and/or Tri-B administration following Epi improved partially the decrease in body weight and liver glycogen levels. Tri B injection following Epi partialy improved all the tested parameters except malondialdehyde and blood sugar level that completely improved in stressed rats. It was evident that a combination of MT and vitamin B complex had protective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; Epinephrine; Melatonin; Tri-B; Rats.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300007

Biosurfactant Production by Bacillus strains isolated from sugar cane mill wastewaters
Provedor de dados: 52
Autores: Santos,Elane Cristina Lourenço dos; Miranda,Daniele Alves dos Reis; Silva,Amanda Lys dos Santos; López,Ana Maria Queijeiro.
Abstract Biosurfactants possess diverse chemical properties and provide important characteristics to the producing microorganisms, which can act as surface-actives and emulsifiers of hydrocarbon and others water insoluble substances. Most of them are lipopeptides synthetized by Bacillus. This study evaluated the biosurfactant production by strains of Bacillus previously obtained from liquid residues of sugar-alcohol industry. The bacterial isolates LBPMA: BSC, BSD, J1, J2 and L1 were cultivated in medium that induces production of biosurfactants (Landy medium). During 48 h of incubation, at intervals of 12 h, the total contents of proteins, reducing carbohydrates and surfactant activity of the filtrated growth media free of cells were evaluated. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emulsification; Oil dispersion; Lubricant oil waste; Bioremediation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100700

Erratum: Effect of LPS on the Viability and Proliferation of Human Oral and Esophageal Cancer Cell Lines
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100901

Urban secondary sewage: an alternative medium for the culture of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae)
Provedor de dados: 52
Autores: Costa,Rauquírio André Albuquerque Marinho da; Koening,Maria Luise; Macedo,Sílvio José de.
The effect of different concentrations (10, 20, 30 and 40%) of urban secondary sewage on the growth of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae) was examined to verify the possibility of its use as an alternative culture medium for these species. Stocks and 700mL duplicate batch cultures were maintained under controlled laboratory conditions. 30% and 40% additions were the most efficient for the growth of both species. Statistical analysis of the adjusted growth (cell.mL-1) and biomass (µg chlorophyll-a.mL-1) curves performed using the Chi-square test (p&lt;0.05) demonstrated significant differences between the nutritive medium f/2 and the 40% addition used for these two species. The results suggested that some of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalgae; Culture; Urban secondary sewage; Alternative medium.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300016

Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation
Provedor de dados: 52
Autores: Mello,Juliana Iura de Oliveira; Barbedo,Claudio José; Salatino,Antonio; Figueiredo-Ribeiro,Rita de Cássia Leone.
Considering the importance of water content for the conservation and storage of seeds, and the involvement of soluble carbohydrates and lipids for embryo development, a comparative study was carried out among the seeds of Inga vera (ingá), Eugenia uniflora (pitanga), both classified as recalcitrant, and Caesalpinia echinata (brazilwood) and Erythrina speciosa (mulungu), considered as orthodox seeds. Low concentrations of cyclitols (0.3-0.5%), raffinose family oligosaccharides (ca. 0.05%) and unsaturated fatty acids (0-19%) were found in the seeds of ingá and pitanga, while larger amounts of cyclitols (2-3%) and raffinose (4.6-13%) were found in brazilwood and mulungu, respectively. These results, in addition to higher proportions of unsaturated fatty acids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desiccation tolerance; Fatty acids; Seeds; Starch; Sugars.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400019

Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium
Provedor de dados: 52
Autores: Kumar,Muthusamy Ashok; Anandapandian,Kanapathi Thangavel Kasirajan; Parthiban,Karuppiah.
Marine water was used for the biofilm forming bacterial isolation. A biofilm forming device was made by using PVC pipes with sterile glass slides. Five morphologically different bacteria were isolated from the scrapping of glass slides from the device after a month period of time. The efficiency of EPS production was checked for all the isolates. Using scanning electron microscope (SEM), the morphology of microbial cells and colonies was studied. The maximum EPS was produced by the strain B3, which was analyzed and characterized by thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) spectra. B3 EPS displayed a strong absorption band of - OH at 3415.31 cm-1 and COOH at 1631.48 cm-1 showing it to be polysaccharide.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Marine Bacteria; EPS; SEM; TLC and FTIR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200006

Alternative invitro propagation: use of sugarcane bagasse as a low cost support material during rooting stage of strawberry cv. Dover
Provedor de dados: 52
Autores: Mohan,Radjiskumar; Chui,Eduardo Assami; Biasi,Luis Antonio; Soccol,Carlos Ricardo.
The purpose of this work was to reduce the cost and improve the quality of the plant material during the micropropagation process in tissue culture. Partially improvement in the rooting process, coupled with cost reduction was obtained during the invitro rooting by the use of a natural support based on sugarcane bagasse as a substitute for the traditionally used agar gelled medium. The tests were conducted with micro-cuttings of strawberry cv. Dover using a medium composed of half strength MS medium (1962), 3% sucrose and 0.05 BAP mg.l-1. The roots number, shoots number, length, and the height of aerial part of 8 independent plants were recorded after 10, 20, 30, and 40 days of culture. Also, an acclimatization tests were realized of the rooted shoots from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Invitro rooting; Alternative support; Strawberry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400005

Use of otoliths to estimate size at sexual maturity in fish
Provedor de dados: 52
Autores: Agostinho,Carlos Sérgio.
The viability of an alternative method for estimating the size at sexual maturity of females of Plagioscion squamosissimus (Perciformes, Sciaenidae) was analyzed. This methodology was used to evaluate the size at sexual maturity in crabs, but has not yet been used for this purpose in fishes. Separation of young and adult fishes by this method is accomplished by iterative adjustment of straight-line segments to the data for length of the otolith and length of the fish. The agreement with the estimate previously obtained by another technique and the possibility of calculating the variance indicates that in some cases, the method analyzed can be used successfully to estimate size at sexual maturity in fish. However, additional studies are necessary to detect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Otolith; Sexual maturity; Plagioscion squamosissimus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400014

Mesenchymal Stromal Cells Modulate PAF-stimulated Equine Alveolar Macrophages
Provedor de dados: 52
Autores: Michelotto,Pedro Vicente; Bastos,Fernanda Zettel; Barussi,Fernanda Cristina Mendes; Senegaglia,Alexandra Cristina; Nishiyama,Anita.
Abstract Platelet-activating factor (PAF) is a potent proinflammatory mediator that is produced in increased amounts in the lungs of asthmatic humans and horses. The present pilot study, shows that mesenchymal stromal cells can modulate alveolar macrophage function in asthma, interfering in the activity of PAF, being another potential pathway for mesenchymal stromal cells benefits in asthma.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Horses; Lung; Platelet-activating factor; Pulmonary inflammation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100306

Estrutura populacional do Camarão Sete-Barbas Xiphopenaeus kroyeri (Heller, 1862), na Foz do Rio Itajaí-Açú, Itajaí, SC, Brasil
Provedor de dados: 52
Autores: Branco,Joaquim Olinto; Lunardon-Branco,Maria José; Souto,Flávio Xavier; Guerra,Cláudio Rogério.
Xiphopenaeus kroyeri is the most exploited shrimp specie along Center-South coast of Santa Catarina State, Brazil, by artisanal fishery. In the period from March/1996 to February/1997, 3965 shrimps were collected (1843 males and 2122 females) sampled by over-trawl, with the depth oscillating between 5 and 15 meters. Xiphopenaeus kroyeri shows seasonal fluctuations in its abundance with higher rates in autumn. The population's average total-length varied between 7.5cm and 9.1cm, and the presence of juveniles was common along the year. The males and females get to the first gonadal maturation with 7.4 cm and 9.0 cm length, respectively; these lengths were reached within 6 and 8 months of age.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xiphopenaeus kroyeri; Population structure; Sea-bob-shrimp.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100016

Occupation pattern of a harbor inlet by the estuarine dolphin, Sotalia guianensis (P. J. Van Bénéden, 1864) (Cetacea, Delphinidae)
Provedor de dados: 52
Autores: Cremer,Marta Jussara; Simões-Lopes,Paulo César; Pires,José Salatiel Rodrigues.
The occupation pattern of Sotalia guianensis in São Francisco do Sul harbor inlet, in Babitonga bay, southern Brazil, was studied between September 1996 and June 1998. A total of 200 h of naturalistic observations and 141.2 h of estuarine dolphin systematic observations were made using binoculars 7 x 50. At each three minutes interval, data about number of individuals and behavior were registered. The population used the harbor inlet intensively, mainly for fishing activities. Ebb tide was responsible for a higher occupation index. Considering the months analyzed, the higher occupation index occurred in May, and in January the lower occurrence was observed. The mean group size was four individuals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sotalia guianensis; Occupation index; Babitonga bay; Harbor use; Behavior.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300029

Rearing of Thyrinteina arnobia (Lepidoptera: Geometridae) on guava and Eucalyptus in laboratory
Provedor de dados: 52
Autores: Oliveira,Harley Nonato de; Zanuncio,José Cola; Pedruzzi,Eder Pin; Espindula,Marcelo Curitiba.
Thyrinteina arnobia, is one of the most important defoliating caterpillars of Eucalyptus in Brazil The objective of this work was to evaluate the development and the reproduction of feeding on leaves of guava (Psidium guajava) or Eucalyptus grandis in the 15th generation, after rearing this species for 14 genrations on guava leaves. T. arnobia showed shorter larval period, better viability of caterpillars and pupae, heavier pupae, higher number of eggs per female, better egg viability and shorter longevity of females in guava leaves than in Eucalyptus leaves. The better development and reproduction with P. guajava showed that this insect could be reared in laboratory with guava leaves.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psidium guajava; Defoliator; Mass rearing; Host; Eucalyptus grandis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600016

Current status of rodenticide intoxication in Brazil: a preliminary survey from 2009 to 2011
Provedor de dados: 52
Autores: Papini,Solange; Nakagawa,Lia Emi.
The chemical control of rodents with anticoagulant products, especially derivatives of the coumarin chemical group, is legally authorised in Brazil. These products provide effective control and are safe for humans. However, the use of illegal 'rodenticides' has increased in many Brazilian cities recently, accompanied by increased numbers of suicides, homicides, and intoxications. The National Toxicology Information System (Sinitox) registers the number of rodenticide intoxications, including suicides, but does not differentiate between the legal and illegal rodenticides. Intoxications by rodenticides outnumber those by illicit drugs and pesticides. A survey of data from 2005-2011 revealed an average of about 3,800 intoxications per year, about 60% of which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coumarin; Aldicarb; Paraffin block; Pellet; Powder; 'Chumbinho'.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500685

Production of L(+) lactic acid using Lactobacillus casei from whey
Provedor de dados: 52
Autores: Panesar,Parmjit S.; Kennedy,John F.; Knill,Charles J.; Kosseva,Maria.
The aim of this work was to study the fermentation of whey for the production of L(+) lactic acid using Lactobacillus casei. The effect of different process parameters such as pH of the medium, temperature, inoculum size, age of inoculum, agitation and incubation time was monitored to enhance the lactose conversion in whey to L(+) lactic acid. Fermentations were performed without any pH control. The optimization of the fermentation conditions resulted in significant decrease in fermentation time, besides increase in lactose conversion to lactic acid. The optimized process conditions resulted in high lactose conversion (95.62%) to L(+) lactic acid production (33.73 g/L) after an incubation period of 36 h.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whey; Lactic acid; Lactose utilization; Lactic acid bacteria; L. casei.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100027

Mosquitos dendrícolas (Diptera, Culicidae) em internódios de taquara da Floresta Atlântica, Serra do Mar e do Primeiro Planalto, Paraná, Brasil
Provedor de dados: 52
Autores: Lozovei,Ana Leuch.
During two consecutive years, from january 1985 to december 1986, studies were carried out on mosquitoes living in the internodes of bamboo in two distinct regions, the Atlantic Forest of the Serra do Mar and the First Plateau, both in the state of Paraná, Brazil. These dendricolous habitats are very different from other recorded mosquito breeding sites. The internodes of the green, living bamboo are closed reservoirs of water with only some small, lateral holes made by boring insects Eucalyptra barbara Schaus, 1894 and Eucalyptra fumida Schaus, 1894 (Lepidoptera, Noctuidae), and other animals. The water in the internodes derives from the bamboo itself. These are the first observations of this breeding behavior. Some females entirely enter the hole to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culicidae; Diptera; Ecologia; Mosquitos dendrícolas; Taquara.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400016

Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1
Provedor de dados: 52
Autores: Geyikoglu,Fatime; Türkez,Hasan.
The aim of this study was to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). Results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared with controls. When sodium selenite (Na2SeO3) was added at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin B1; Selenium; Sister-chromatid exchanges; Genotoxicity; Blood.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800006

Morpho-Anatomical Analysis of the Rhizome in Species of Scleria Berg. (Cyperaceae) from Serra do Cipó (MG)
Provedor de dados: 52
Autores: Lima,Vera Fatima Gomes Alves Pereira; Menezes,Nanuza Luiza de.
Aspects related to the nature of stem thickening in monocotyledons have been the subject of many studies. Primary thickening has been attributed to the Primary Thickening Meristem (PTM). According to most authors, it gives rise, besides the adventitious roots, to the vascular tissues and part of the cortex. In other words, it has centripetal and centrifugal activity. For some authors, however, it gives rise only to the vascular system, and for others, only to part of the cortex. However, this work demonstrated that PTM corresponds to the pericycle in the meristematic phase or to the pericycle associated with the endodermis, also with meristematic activity. It was observed that the pericycle was responsible for the formation of the vascular system of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meristematic endodermis; Rhizome; Cyperaceae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600019

Population Structure and Genetic Diversity of Sweet Cassava Accessions from the Midwestern, Southeastern and Southern Regions of Brazil
Provedor de dados: 52
Autores: Ortiz,Alex Henrique Tiene; Vidigal Filho,Pedro Soares; Rocha,Vanesca Priscila Camargo; Ferreira,Rebecca Caroline Ulbricht; Gonçalves,Tiago Maretti; Gonçalves-Vidigal,Maria Celeste.
Abstract The objective of this work was to screen sweet cassava accessions collected in smallholding areas in the Midwestern, Southeastern and Southern regions of Brazil, using 15 SSR molecular markers, to determine population structure and genetic diversity. Polymorphism was detected in every loci analyzed, with mean of 6.33 alleles per locus, and mean polymorphism information content (PIC) of 0.6057, pointing out that the primers were highly informative. The observed heterozygosity ranged from 0.0709 (SSRY 101) to 0.9398 (GA 12), with a mean of 0.6511, and mean genetic diversity of 0.6578, ranging from 0.3592 (GA 136) to 0.8116 (SSRY 21). The most dissimilar combinations observed were BGM526PR-BGM596MS, BGM526PR-BGM622MS and BGM526PR-BGM629MS. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Germplasm characterization; Genetic variability; SSR markers.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100206

Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment
Provedor de dados: 52
Autores: Bespalhok Filho,João C.; Kobayashi,Adilson K.; Pereira,Luiz F. P.; Galvão,Rafaelo M.; Vieira,Luiz G. E.
Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transformation; UidA expression; Biolistics; Transgenic citrus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100001

Correlation between the length and weight of Arius maculatus off the southwestern coast of Taiwan
Provedor de dados: 52
Autores: Chu,Wu-Shan; Hou,Yi-You; Ueng,Yih-Tsong; Wang,Jiang-Ping.
In this study, Arius maculatus were collected using the samples caught by bottom trawling off the southwestern coast of Taiwan. Length-weight and length-length, fork (FL), standard (SL) and total (TL) lengths, relationships were determined. The relationships between the lengths were all significantly linear (p&lt;0.01), the b value in the length-weight relationship for this value was significantly lower than 3 in the fall (p&lt;0.01), when the temporal changes were taken into account, indicating that only the sampling time affected the growth pattern of A. maculatus. The growth was isometric in the spring, summer and winter, but it was negative and allometric in the fall.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arius maculatus; Length-Weight Relationship; Growth; Stand Length (SL).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500009

Response of cattleya hybrids for Fusarium oxysporum f. sp. cattleyae Foster
Provedor de dados: 52
Autores: Pedroso-de-Moraes,Cristiano; Souza,Marcelo Claro de; Ronconi,Cínthia Cristina; Marteline,Marco Aurélio.
The Cattleya genus has a great importance in the flower agro-business market. Fusarium wilts, caused by Fusarium oxysporum f. sp. cattleyae, is considered one of the main factors of decline and death of plants of this genus. Using seven hybrids (intra and intergenerics) of Cattleya, tests of resistance and susceptibility to F. oxysporum were performed in conditions of greenhouse for 12 months, using, as evaluation criterion, a scale of the disease severity ranging from one (resistant) to eight (highly susceptible). High susceptibility to the fungus by Cattleya Nobile's Wax Toy, Cattleya Orquidacea's Mister Fast intrageneric hybrids and Potinara Orquidacea's Havana Brown intergeneric hybrid, related to Brassocattleya Orquidacea's Melody intergeneric hybrid,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium wilts; Orchid; Varietal resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200007

Behavior of the combined radial post-feeding larval dispersal of the blowflies Chrysomya megacephala and Chrysomya albiceps (Diptera, Calliphoridae) and implications for forensic entomology
Provedor de dados: 52
Autores: Gomes,Leonardo; Sanches,Marcos Rogério; Von Zuben,Claudio José.
The aims of this work was to study the life stages of Chrysomya megacephala in a circular arena allowing the combined radial post-feeding dispersal from the center of the arena of C. albiceps and C. megacephala larvae. To determine the location of each pupa, the distance from the center, the depth and weight of each pupa formed were analyzed. For the larvae of C. albiceps, females released buried deeper than males, reaching an average depth of 10.74 cm and C. megacephala larvae presented greater average results than C. albiceps for all variables, particularly distance, which was 16.02 cm for this species and 12.30 cm for C. albiceps. C. albiceps larvae preyed on 30 C. megacephala larvae in both experiments. These results could be used in forensic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blowflies; Calliphoridae; Dispersal; Forensic Entomology; Chrysomya albiceps; Chrysomya megacephala.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200013

Caloric density of aquatic macrophytes in different environments of the Baía river subsystem, upper Paraná river floodplain, Brazil
Provedor de dados: 52
Autores: Lopes,Célia de Almeida; Faria,Anna Christina Esper Amaro de; Manetta,Gislaine Iachstel; Benedito-Cecilio,Evanilde.
The aim of this work was to determine the caloric density of leaves, stems and roots of aquatic macrophytes in different environments of the Baía subsystem (Baía river and Fechada and Guaraná lagoons) on the Upper Paraná river floodplain, in addition to identify the variability between ecological groups. Samplings of Eichhornia crassipes, Salvinia spp, Pistia stratiotes, Eichhornia azurea, Polygonum sp, Cyperaceae and Poaceae were carried out in February 2003. Spatial differences in the caloric densities were not observed for these plants. Caloric density values varied from 1906.9 cal/g dry weight (root) to 4675.0 cal/g dry weight (leaf). However, significant differences between the caloric content averages of the vegetative structures were observed only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paraná river; Floodplain; Aquatic macrophytes; Calorimetry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600018

Scintigraphic diagnosis of infectious complications in renal failure patients undergoing hemodialysis, continuous ambulatory peritoneal dialysis or renal transplant
Provedor de dados: 52
Autores: Vicente,Ana María García; Solís,Sebastián Ruiz; Castrejón,Angel Soriano; García,Víctor Manuel Poblete; Rubio,Maria del Prado Talavera; Marina,Sonia Rodado; Romera,Montserrat Cortés.
Patients with end-stage renal disease have two therapeutic options, dialysis and renal transplantation. Infectious complications occurring in such patients will not only condition the effectiveness of such treatments, but are among the main causes of morbidity and mortality in such cases. Knowledge of the advantages and limitations of nuclear techniques is essential for management of these conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious complications; Hemodialysis; Peritoneal dialysis; Renal transplantation; 67Gallium; Labeled leukocytes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700015

Cat, cougar, and jaguar spermatogenesis: a comparative analysis
Provedor de dados: 52
Autores: Costa,Deiler Sampaio; Paula,Tarcízio Antônio Rêgo; Matta,Sérgio Luiz Pinto da.
This work presents a comparative review about the spermatogenic process in cats (Felis domestica), jaguars (Panthera onca) and cougars (Puma concolor), with emphasis on testicular biometry, gonadossomatic index, volumetric proportion of testicular parenchyma components, tubular diameter, seminiferous epithelial height and seminiferous tubule length. It was an approach of the differences among the cell proportions that allowed conclusions about the overall yield of spermatogenic process and Sertoli cell index in three feline species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogenesis; Felines; Testis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600006

Investigation on chemical composition and optimization of essential oil obtainment from waste Pinus taeda L. using hydrodistillation
Provedor de dados: 52
Autores: Teixeira,Sirlei Dias; Fiorio,Jhonatan Luiz; Galvan,Diego; Sefstrom,Carolina; Cogo,Priscila Morgana; Sales Junior,Valber; Rodrigues,Márcio Barreto; Hendges,Ana Paula Palaro Klein; Maia,Beatriz Helena L. de Noronha Sales; Benghi,Thalita Gilda Santos.
ABSTRACT: The extraction of essential oils obtained by the hydrodistillation of needles/twigs waste of Pinus taeda L. was optimized by applying response surface methodology (RSM), with 24 full factorial design, in order to improve oil essential production, and aggregate value to the production chain of pine wood. Through the model it was possible to ascertain the influence of the variables in the average amount of essential oil (0.1032 mL), being the variables analyzed: biomass - Bm (x1), extraction time - ET (x2), Bm:ET (x1x2) and sample size - SS: drying times - DT (x3x4). Only linear terms (biomass and extraction time) and your interaction demonstrated significant positive values (0.0344, 0.0206 and 0.0131). The major components of the essential oil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pinus; Byproduct; Essential oil; Response surface methodology (RSM); Mathematical modeling.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100603

Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14
Provedor de dados: 52
Autores: Carvalho,André Lacerda Ulysses de; Oliveira,Fábio Henrique Portella Corrêa de; Mariano,Rosa de Lima Ramos; Gouveia,Ester Ribeiro; Souto-Maior,Ana Maria.
The physiology of B. subtilis R14 was investigated in minimal medium under excess-oxygen and oxygen-limited conditions. Growth and efficient sporulation could be achieved in excess-oxygen culture on medium with readily metabolizable carbon and nitrogen sources, which allowed high growth rate and high biomass yield. A short transition phase between the exponential growth and sporulation could be attained by formulating a medium with a well-balanced C/N ratio. Under oxygen-limitation, but in the presence of essential nutrients (i.e. excess-nutrient cultivation), B. subtilis R14 produced bioactive compounds, which showed activity against several phytopathogenic bacteria. Under anaerobic condition, the organism did not grow neither through fermentation nor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus subtilis; Batch cultivation; Oxygen availability; Antimicrobial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300020

Influence of culture conditions on Vero cell propagation on non-porous microcarriers
Provedor de dados: 52
Autores: Souza,Marta Cristina de Oliveira; Freire,Marcos da Silva; Castilho,Leda dos Reis.
Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are being increasingly used. The use of microcarriers in stirred bioreactors allows high cell densities and, consequently, high virus titres to be achieved. With the aim of selecting appropriate culture conditions for the cultivation of Vero cells at high cell densities, in this work the influence of several variables (agitation rate, ratio of inoculated cells to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vero cells; Microcarriers; Cytodex 1; Cytodex 3; High-cell-density cultivation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400009

Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest
Provedor de dados: 52
Autores: Ghazala,Imen; Haddar,Anissa; Romdhane,Molka Ben; Ellouz-Chaanouni,Semia.
ABSTRACT: This research focused on isolation, identification and characterization of new strains of fungi and bacteria, which were able to produce extracellular xylanase, mannanase, pectinase and α-amylase. Fungi isolates were identified on the basis of analyses of 18S gene sequencing and internal transcribed spacer region. The closest phylogenetic neighbors according to 18S gene sequence and ITS region data for the two isolates M1 and SE were Aspergillus fumigatus and Aspergillus sydowii, respectively. I4 was identified as Bacillus mojavensis on the basis of the 16S rRNA gene sequencing and biochemical properties. The enzyme production was evaluated by cultivating the isolated microorganisms in liquid-state bioprocess using wheat bran as carbon source....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylanase; Pectinase; Β-mannanase; Α-amylase; Bacillus mojavensis; Aspergillus fumigatus and Aspergillus sydowii.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100510

Cefuroxime axetil loaded gastroretentive floating tabletsbased on hydrophilic polymers: preparation and in vitro evaluation
Provedor de dados: 52
Autores: Mohapatra,Snehamayee; KumarKar,Rajat; Mohapatra,Debendra Kumar; Sahoo,Sunit Kumar; Barik,Bhakti Bhusan.
The aim of this work was to study the formulation and in vitro characterization of hydro dynamically balanced floating matrix tablets using Cefuroxime axetil (CA) as model drug. Different excipients such as hydroxy propyl methyl cellulose (HPMC) K15M, E5LV (gelling agent), sodium bicarbonate (gas generating agent) and sodium lauryl sulfate (SLS) (solubility enhancer) were used in order to optimize the drug release profile as well as floating property. Decrease in release characteristics with high viscous polymer were observed due to increased gel strength, tortuosity and length of drug diffusion path. Significant difference (p&lt;0.5) in release rate was found at different concentration of SLS. The release mechanisms were explored and explained with zero...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cefuroxime Axetil; HPMC; SLS; Release kinetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200013

Evaluation of reproductibility and detection limit of CaSO4: dy radiation detectors
Provedor de dados: 52
Autores: Manzoli,José Eduardo; Campos,Vicente de Paulo de; Doi,Mirian Saori.
Measurement response of thermoluminescent dosimeter, TLD, used by workers or placed at positions where gamma radiation field could be in action affecting biological tissues, should be completely characterized, in order to achieve the radiation quantity with precision and confidence. Among the evaluations concerned to its characterization, the detector reproductibility is of fundamental importance, because detectors present inside the TLD will be used many times in routine. Reproductibility is studied by repeated exposure to the same radiation field. The minimum detection limit is another important characteristics of a TLD. In this work evaluations of reproductibility and minimum detection are presented, for dosimeters produced at IPEN.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detector selection; Personal dosimetry; Environmental dosimetry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200009

The potential of denitrification for the stabilization of activated sludge processes affected by low alkalinity problems
Provedor de dados: 52
Autores: Hoffmann,Heike; Costa,Tatiana Barbosa da; Wolff,Delmira Beatriz; Platzer,Christoph; Costa,Rejane Helena Ribeiro da.
In this study, the problems provoked by nitrification of wastewater with low alkalinity were analyzed in a pilot sequencing batch activated sludge reactor (SBR). Decrease in pH resulted in disappearence of protozoa. De-flocculation of the activated sludge floc started below pH 6.5, resulting in enhanced effluent turbidity and loss of bacteria. Nitrification efficiency was affected below pH 6.2. The denitrification activity was not sufficient to keep up the pH, due to a low C/N ratio of the wastewater. Based on alkalinity and ammonia concentration of the wastewater and the necessary denitrification rate to prevent operational problems, was developed a prognostic diagram. The applicability of this diagram was tested for the SBR with excellent results. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkalinity; Activated sludge; Nitrification; Denitrification.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200018

Side-effects of pesticides used in the organic system of production on Apis mellifera Linnaeus, 1758
Provedor de dados: 52
Autores: Efrom,Caio Fábio Stoffel; Redaelli,Luiza Rodrigues; Meirelles,Rafael Narciso; Ourique,Cláudia Bernardes.
This study aimed to evaluate the effects of pesticides, used in the organic system, on Apis mellifera under laboratory conditions. Four multiple (0.25x, 0.5x, 1x and 2x) concentrations as recommended by they manufacturers of the following products: Rotenat CE®, Pironat®, Biopirol 7M®, Organic neem®, Natuneem® and lime sulfur were tested by topical application and ingestion. Of all the products and concentrations tested, only the lime sulfur (5000 ml 100L-1 and 10000 mL 100L-1 of water) by ingestion, and Rotenat CE® (1200ml 100L-1 of water) on topical application were considered slightly harmful for A. mellifera, as the classification of IOBC/WPRS for the laboratory tests.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Honeybees; Rotenone; Lime sulfur; Neem; Pyroligneous extract.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100005

Erratum: Diversity of the Potential 2-Methylisoborneol-Producing Genotypes in Thai Strains of Planktothricoides (Cyanobacteria)
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100909

Action of the extracts of Pluchea sagittalis on the absorptive characteristics of the gastrointestinal tract
Provedor de dados: 52
Autores: Burger,Marilise E.; Baldisserotto,Bernardo; Teixeira,Elisângela P.; Soares,Joceana.
Pluchea sagittalis (Lam.) Cabrera, (quitoco or erva lucera) is commonly used to treat digestive disorders in Southern Brazil and countries of the South Cone. The crude aqueous extracts from the leaves, stalks or flowers were used in acute oral toxicity in mice and in pharmacological studies to determine the gastrointestinal transport of water, sodium, and potassium in rats. The oral administration of 5000 mg/kg of extracts examined did not produce signs of intoxication nor induce the death of any mice during the period of 14 days. The extracts from the leaves and stalk have reduced the absorption of water in the jejunum and jejunum and ileum, respectively with relation to the control. There was an absorption of sodium with the administration of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pluchea; Ion transport; Gastrointestinal tract absorption; Acute toxicity.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100012

Detection and identification of TMV infecting tomato under protected cultivation in Paraná State
Provedor de dados: 52
Autores: Silva,Rodrigo Martins da; Souto,Eliezer Rodrigues de; Pedroso,Júlio Cézar; Arakava,Ricardo; Almeida,Álvaro Manuel Rodrigues; Barboza,Antônio Augusto Lazarini; Vida,João Batista.
During an inspection in plastic houses in Sapopema, Paraná, 90% of tomato plants showed leaf abnormalities, probably associated with herbicide toxity. However, virus like symptoms developed in selected hosts after mechanical inoculatation. RT-PCR reactions using primers for an internal region within the movement protein gene of TMV and ToMV resulted in the amplification of a 409 bp cDNA fragment only by TMV primers. Deduced amino acids showed 100% identity when compared to TMV movement protein and 94% with ToMV. The RT-PCR protocol was efficient for quick and conclusive determination of virus species. The virus was purified and a polyclonal antiserum was raised for future surveys in tomato crops of Paraná. The partial genomic sequence obtained for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TMV; P30 protein; Plasticulture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500005

Treatment of shrimp effluent by sedimentation and oyster filtration using Crassostrea gigas and C. rhizophorae
Provedor de dados: 52
Autores: Ramos,Roberto; Vinatea,Luis; Seiffert,Walter; Beltrame,Elpídio; Silva,Júlia Santos; Costa,Rejane Helena Ribeiro da.
Efficiency in removing particulate matter from Litopenaeus vannamei shrimp culture effluent was assessed in laboratory scale employing sedimentation and oysters Crassostrea gigas and C. rhizophorae filtration processes. Cylindroconical tanks (100 L) were used in duplicate for sedimentation and 50-L in triplicate for oyster filtration. Fifteen oysters of each species weighing 76-80 g were stocked in each of the filtration treatment experimental units (biomass of 1065 - 1174 g oyster per unit). The control treatment was a tank similar to those used in the filtration treatment but with empty oyster shells. Hydraulic retention time of the effluent was of 6 hours in each treatment. First, effluent went through sedimentation, and then the supernatant went...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oysters; Filtration; Sedimentation; Effluent; Litopenaeus vannamei.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300030

Isolation and Identification of Novel IndigenousBacterial Strain as a Low Cost Pectinase Source
Provedor de dados: 52
Autores: Bibi,Nowshad; Ali,Sikander; Tabassum,Romana.
ABSTRACT The present study was concerned with the searching of novel bacterial cultures from different samples for the lab scale production of pectinase. Keeping in view the increasing demand of pectinase specially in Faisalabad, an industrial city of Pakistan, isolation of new hyper producer bacterial strains locally is an easy and cheap way of getting the desirable products at low cost. Therefore, isolation of new strains for industrial enzyme production has been and will be remained a part of research every time. This method alone can also provide raw material for further research such as enzyme engineering or molecular directed evolution. For the identification of hyper producer strain colony PCR was done for 16S rRNA analysis. Reason to use the 16S...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indigenous; Low cost; Colony PCR; Bacillus licheniformis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100424

Control of papaya fruits anthracnose by essential oil of Ricinus communis
Provedor de dados: 52
Autores: Siqueira Júnior,César Luis; Freire,Maria das Graças Machado; Moreira,Antônio Sérgio Nascimento; Macedo,Maria Ligia Rodrigues.
The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness of castor oil for the control of anthracnose was shown when 5% and 10% (v/v) were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v) concentration of essential oil. The studies with the fresh fruits treated with 5% (v/v) castor oil in aqueous emulsions resulted in effective reduction of pathogen spread in these fruits. No lesion was found in the fruits treated with oil, when compared to the control...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Antimicrobial activity; Essential oil; Postharvest; Colletotrichum gloeosporioides; Papaya.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100009

Effect of spray volume on the moisture of stored corn and wheat grains
Provedor de dados: 52
Autores: Vásquez-Castro,Javier Alberto; Baptista,Gilberto Casadei de; Gadanha Junior,Casimiro Dias; Trevizan,Luiz Roberto Pimentel.
The goal of this work was to evaluate the effect of spray volume on the moisture of the stored grains of the corn and wheat. Two kg of each type of the grain were placed into the plastic bags and sprayed with the theoretical doses of 0, 1, 3, 5, 8, and 10 liters of water / ton of the grain. The grain moisture content was evaluated 24 h after the spray operation by the oven method. The increase in the grain moisture was quadratic and showed the same trend in both the corn and wheat. The grain moisture after spraying 10 L.t-1 showed little increase (0.8 %) as compared to the initial moisture content. Thus, the application of any spray volume as used in this study made no difference for a possible better uniformity in the distribution of insecticide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stored products; Chemical control; Application technology; Desorption; Hygroscopic equilibrium.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300003

Influence of edapho-climatic factors on the sporulation and colonization of arbuscular mycorrhizal fungi in two Amazonian native fruit species
Provedor de dados: 52
Autores: Oliveira,Arlem Nascimento de; Oliveira,Luiz Antonio de.
Arbuscular mycorrhizal fungi (AMF) colonization and spore numbers in the rhizosphere of two fruit species, Paullinia cupana Mart. and Theobroma grandiflorum Schum., growing in a terra firme ecosystem in Central Amazonia were studied from August 1998 to May 2000. Climatic and edaphic factors were also determined to investigate their influence on mycorrhizal variables. Soil pH, Al, Mn and effective cation exchange capacity exhibited seasonal variations during the investigation period. Temporal variations in mycorrhizal colonization levels and spore numbers occurred, indicating seasonality. Moreover, the patterns of mycorrhizal colonization levels and spore numbers for both host species were similar during the studied period. Mycorrhizal variables were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arbuscular mycorrhizal fungi; P. cupana; T. grandiflorum; Central Amazonia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300021

Effect of antimalarial drugs on the bioavailability of the methylenediphosphonic acid labeled with technetium99m (99mTc-MDP) in Wistar rats
Provedor de dados: 52
Autores: Holanda,Cecília Maria de Carvalho Xavier; Leite,Rodrigo de Carvalho Holanda; Nunes,Reginauro Alves da Silva; Oliveira,Harina Alves de; Catanho,Maria Teresa Jansen de Almeida; Souza,Grace Mary Lima de; Bernardo-Filho,Mário.
The aim of this work was to study the effect of antimalarial drugs on the bioavailability of 99mTc-MDP in rats. Mefloquine (MQ) and artemisinin (AM) were administered in two treated groups (T) and sorbitol in control group (C) for 7 days. Then, 99mTc-MDP was injected in all groups and %ATI was calculated. A significant increase of %ATI in MQ group, from C to T, occurred in spleen (0.35±0.10to0.58±0.13), liver (1.69±0.28to3.31±0.07) and blood (0.79±0.17to2.09±0.53). The %ATI increased significantly in AM group:femur (2.76±0.59to5.98±0.70), liver (1.69±0.28to4.59±0.68), lungs (0.29±0.05to6.22±0.86), spleen (0.35±0.10 to0.86±0.15) and blood (0.79±0.17 to4.65 ±0.74). A significant decrease of %ATI occurred in MQ group:bladder (0.75±0.07to0.26±0.05), stout...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug interaction; Radiopharmaceuticals; 99mTc-MDP; Antimalarial drugs; Mefloquine; Artemisinin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300004

Diet and prey availability of terrestrial insectivorous birds prone to extinction in amazonian forest fragments
Provedor de dados: 52
Autores: Mestre,Luiz Augusto Macedo; Cohn-Haft,Mario; Dias,Manoel Martins.
This study compared niche breath, prey size, and diet variability in two pairs of sympatric species of terrestrial insectivorous birds, each pair containing one species that can persist in small forest fragments and one that does not. The pairs were Myrmeciza ferruginea and Sclerurus rufigularis; and Formicarius colma and F. analis, respectively. The prey availability in forest fragments was also sampled and compared to the availability in continuous forests. Niche breath indices did not differ between pair members, but diet variability differed in the opposite direction from that hypothesized. Although the two bird species most vulnerable to fragmentation fed on larger prey than less vulnerable species, prey availability, including that based on prey size...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian forests; Bird diet; Forest fragmentation; Insectivores; Formicarius colma; Formicarius analis; Myrmeciza ferruginea; Sclerurus rufigularis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600014

Percutaneous absorption enhancers: mechanisms and potential
Provedor de dados: 52
Autores: Carpentieri-Rodrigues,Letícia Norma; Zanluchi,Juliana Modolo; Grebogi,Ivanna Hinke.
Transdermal applications of drugs present many advantages in terms of absorption, however this is not easily obtained through the transdermal route. The principle barrier is the stratum corneum and one of the strategies that have been found to promote cutaneous drug penetration is through the use of absorption enhancers. Many substances have been identified as absorption enhancers. Although the list of substances that promote absorption is growing, in most cases, there is a direct correlation between the effects of absorption enhancers and their skin toxicity. The use of these substances depends therefore on studies which focus on local and systemic toxicity, as well as action mechanisms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Absorption enhancers; Cutaneous penetration; Percutaneous absorption.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700006

Research on manufacturing text classification based on improved genetic algorithm
Provedor de dados: 52
Autores: Kaijun,Zhou; Yifei,Tong.
ABSTRACT According to the features of texts, a text classification model is proposed. Base on this model, an optimized objective function is designed by utilizing the occurrence frequency of each feature in each category. According to the relation matrix oftext resource and features, an improved genetic algorithm is adopted for solution with integral matrix crossover, transposition and recombination of entire population. At last the sample date of manufacturing text information from professional resources database system is taken as an example to illustrate the proposed model and solution for feature dimension reduction and text classification. The crossover and mutation probabilities of algorithm are compared vertically and horizontally to determine a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Text classification; Genetic algorithm; Dimension reduction; Text classification; Manufacturing text.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200600

Glycemic index: effect of food storage under low temperature
Provedor de dados: 52
Autores: Carreira,Marina Cassab; Lajolo,Franco Maria; Menezes,Elizabete Wenzel de.
This study was carried out to evaluate the influence of food storage under low temperature (-20ºC) and the resistant starch formation, both on the glycemic index (GI). The GI of only cooked and cooked and stored foods under -20ºC for 30 days was evaluated in short-term tests with humans. Significant increase on the RS content was evidenced for all the stored foods. The food storage resulted in a significant decrease on the GI of beans and chick-peas; the GI of pasta remained the same and the GI of corn meal increased. Thus, the RS formation showed reduced influence on the glycemic index. The storage of starchy foods under low temperature can collaborate to the RS intake but its effect on the GI will depend on the characteristics of the carbohydrates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Starch; Glycemic index; Resistant starch; Storage.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400010

Nodulation and N2 fixation effectiveness of Bradyrhizobium strains in symbiosis with Adzuki Bean, Vigna angularis
Provedor de dados: 52
Autores: Delić,Dušica; Stajković,Olivera; Rasulić,Nataša; Kuzmanović,Djordje; Jošić,Dragana; Miličić,Bogić.
In pot experiment, one isolate Knj from a Serbian soil, four strains of Bradyrhizobium japonicum and three strains of Bradyrhizobium spp. were examined for the effect on adzuki bean nodulation and effectiveness in symbiotic N2 fixation. All the tested strains produced root nodules in adzuki bean. Strains of B. japonicum showed high potential of N2 fixation, particularly 525 and 542. B. japonicum strains resulted 65-71% shoot dry weight and 99-138% total N content of uninoculated control with full N content (100%). No significant difference was found between the plants inoculated with Bradyrhizobium spp. strains and uninoculated control plants without N (40-42 and 42% shoot dry weight, respectively), which indicated symbiotic N2 fixation inactivity of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradyrhizobium; Adzuki bean; Nodulation; Symbiotic N fixation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200007

Indoor Air quality related to occupancy at an air-conditioned public building
Provedor de dados: 52
Autores: Ponsoni,Karina; Raddi,Maria Stella Gonçalves.
To characterize the influence of occupancy on the indoor air quality, a public office building with air-conditioning system was selected for this study. The indoor parameters included total bacteria count, total fungal count, temperature, relative humidity, and carbon dioxide concentration. The number of occupants, which varied throughout the day, was recorded in each sample. The samples were taken before the beginning of the working day and during 3 h, at an interval of 30 min between each sampling, and continued for five working days during a week. Correlation analysis demonstrated that occupancy rates were positively correlated with airborne bacteria, CO2, and temperature. No significant association between the number of occupants and fungus was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indoor air quality; Air-conditioned; Occupancy; Office building.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100013

Biological evaluation of a nutritional supplement prepared with QPM Maize cultivar BR 473 and other traditional food items
Provedor de dados: 52
Autores: Paula,Heberth de; Santos,Rinaldo C.; Silva,Marcelo E.; Glória,Enara C. S.; Pedrosa,Maria Lúcia; Almeida,Nízia A. V.; Costa,Alexandre S. V.; Malaquias,Luiz Cosme C.
Quality Protein Maize (QPM) cultivar BR 473 was employed, together with soybean flour, brown sugar, banana meal and oat meal, for the preparation of a nutritional supplement.. 21-day old male Fisher rats were fed diets containing the supplement as a protein source, both with and without soybean flour; casein diets with 10 or 7% protein served as respective controls. Protein Efficiency Ratio (PER), Net Protein Utilization (NPU), Net Protein Retention (NPR) and Digestibility were determined. Blood biochemical parameters (glucose, cholesterol, urea, hemoglobin, albumin and total protein) were also measured in the animals and showed that all were in good health condition at the end of the experiment. The obtained results for PER, NPU and NPR indicated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QPM Maize; Protein; Biological evaluation; Nutritional supplement.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200012

Common causes of false positive F18 FDG PET/CT scans in oncology
Provedor de dados: 52
Autores: Carter,Kevin R.; Kotlyarov,Eduard.
PET/CT is a common imaging modality used in the evaluation of oncology patients. While being extremely sensitive to identifying sights of malignancy F18FDG is very non-specific. We attempted to provide a brief review of some of the more common processes that a nuclear radiology physician may encounter in daily clinical practice that could result in a false positive diagnosis with F18FDG PET/CT. A fundamental understanding of the limitations of this technology by the interpreting physician is necessary to avoid making inaccurate diagnosis and potentially limiting important treatments for our patients.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PET/CT; False Positives.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600004

Effect of moxifloxacin administration on pharmacokinetics of tolfenamic acid in rats
Provedor de dados: 52
Autores: Patel,Satish D.; Sadariya,Kamlesh A.; Gothi,Anil K.; Patel,Urvesh D.; Gohil,Pradhuman A.; Jain,Mukul R.; Bhavsar,Shailesh K.; Thaker,Aswin M.
Pharmacokinetics of tolfenamic acid as a single drug (4 mg/kg, intramuscularly) and its co-administration with moxifloxacin (5 mg/kg, intramuscularly) in wistar rats were studied. The plasma drug concentration of tolfenamic acid was assayed by LC-MS/MS. Following intramuscular administration of tolfenamic acid as single drug and in combination with moxifloxacin in male rats, the mean values of observed peak plasma drug concentration (Cmax), area under plasma drug concentration-time curve (AUC(0-¥) ), volume of distribution (Vz), half-life (t½) and clearance (Cl) were 4111.44 ± 493.15 and 3837.69 ± 351.83 ng/ml, 20280.77 ± 3501.67 and 15229.18 ± 678.80 ng.h/ml, 822.17 ± 115.38 and 1249.64 ± 139.52 ml, 2.59 ± 0.16 and 3.27 ± 0.32 hr, and 218.39 ± 25.47 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacokinetics; Tolfenamic acid; Anti inflammatory drug; Rats; Interaction; Moxifloxacin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400013

Vegetation and pollen rain relationship from the tropical Atlantic rain forest in Southern Brazil
Provedor de dados: 52
Autores: Behling,Hermann; Negrelle,Raquel R. B.
The relationship between the southern Brazilian tropical Atlantic lowland rain forest and modern pollen rain was studied by pollen traps. The study was carried out on a one hectare plot undisturbed rain forest of the reserve Volta Velha and two secondary forests, ± 50 and 7 years old. About 248 identified tree, shrub and herb species (excluding epiphytes) of 50 families were represented by 126 different pollen and spore types (including non-local taxa). The calculated average influx of pollen rain from the native Atlantic rain forest was 12465 pollen grains per cm² and year. The influx from the ± 50 years old and from the 7 years old secondary forest was relatively low (4112 and 3667 grains per cm² and year, respectively) compared to the undisturbed rain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Southern Brazil; Modern pollen rain; Tropical Atlantic rain forest; Secondary forest.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500013

Comparative biodistribution profile of [131I]VIP and [131I]VIP10-28
Provedor de dados: 52
Autores: Colturato,Maria Tereza; Muramoto,Emiko; Araújo,Elaine Bortoleti de.
Various tumor cells express significantly higher amounts of VIP receptors (VIPR) that provided the basis for the clinical use of radiolabeled VIP for the in vivo localization of tumors. This work studied the labeling of VIP and VIP10-28 with iodine-131 to compare the biological distribution of the labeled compounds in Nuce mice and the affinity for tumor cells. Both VIP and VIP10-28 peptides contain two tyrosine residues, in positions 10 and 22, that are theoretically equally susceptible to radioiodination employing oxidative electrophilic substitution using oxidizing agents like Chloramine T. Radiochemical purity of the reaction mixture was determined by electrophoresis and HPLC. The VIP peptide and the fragment were labeled with radioiodine with good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Protein radioiodination; VIP; VIP10-28; Adenocarcinomas.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700012

Hydration Properties of Soybean Protein Isolates
Provedor de dados: 52
Autores: Remondetto,G.; Añon,M. C.; González,Rolando J.
Hydration properties of soybean isolates with different processing conditions (heat treatments, pH and protein concentrations) were studied. Degree of denaturation,, solubility in water, in 0.2mol/L NaCl, and in media of different sodium dodecyl sulfate concentrations, viscosity and water imbibing capacity of the different samples were determinated and correlated. Treatments at temperatures higher than 80ºC denatured 11S and 7S proteins, leading to exposure of hydrophobic groups, which produced insoluble aggregates either in water or in high ionic strength media. These isolates possessed high water imbibing capacities and gave rise to viscous dispersions. Significant correlations were obtained between hydration properties and the "m" coefficient as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean proteins; Hydration properties; Protein isolates functional properties.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400014

New geometric models for calculation of microalgal biovolume
Provedor de dados: 52
Autores: Konoplya,Bárbara Idino; Soares,Francisco Striquer.
Biovolume calculation of micro algae is commonly used for the assessment in relative abundance (as biomass or carbon) of coexistent algae that vary in shape and/or size. Periphyton samples were obtained within a more general program, in fishery tanks and streams situated in the upper basin region of Tibagi River, nearby Londrina, Paraná state, south of Brazil. The present work proposes new algorithms for the determination of algal species volume. Results showed that Gyrosigma scalproides (Rabenhorst) Cleve 1894 and Tetraedron gracile (Reinsch) Hansgirg 1889 were exceptions inside their genus and the present new formula for biovolume and area gives more adequate results. In addition, new formulas applied for Phacus longicauda (Ehrenberg) Dujardin 1841 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biovolume; Geometric models; Biomass; Micro algae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300014

Optimization of fermentation conditions for cellulases production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from Indian hot spring
Provedor de dados: 52
Autores: Acharya,Somen; Chaudhary,Anita.
The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55°C, respectively. Maximum cellulases production by both the isolates was detected after 60 h incubation period using wheat and rice straw. The combination of inorganic and organic nitrogen source was suitable for cellulases production. Overall, FPase production was much higher than CMCase production by both of the strains. Between the two thermophiles, the cellulolytic activity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Thermophiles; Submerged fermentation; Parameter optimization; Cellulases; Hot spring.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400003

Chemical Composition and Antioxidant Activity of Essential Oil and Organic Extracts of Premna integrifolia Linn
Provedor de dados: 52
Autores: Al-Reza,Sharif M.; Rokonuzzaman,M.; Afroz,Monira; Hussain,Md. Iqbal; Rashid,M. A.; Rahman,Atiqur.
ABSTRACT This study was designed to examine the chemical composition and in vitro antioxidant activity of the hydrodistillated essential oil and various extracts obtained from Premna integrifolia Linn. GC-MS analysis of the essential oil was resulted in determination 29 different compounds, representing 95.73% of total oil. Antioxidant activities of the essential oil and organic extracts of chloroform, ethyl acetate and methanol were determined by three different test systems namely DPPH (2,2-diphenyl-1-picrylhydrazyl), superoxide and nitric oxide radical scavenging assays. The essential oil and methanol extract showed potent antioxidant activity among all the tested samples. Furthermore, the amount of total phenolic compounds was determined and its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premna integrifolia Linn; Antioxidant activity; Essential oil; Total phenolics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100203

ERRATUM: Investigation of Mode of Action of Anti Bacterial Activity of Salacia Oblonga Extract Against Drug Resistant Pathogen
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000101501

Antiproliferative Effect of Urera baccifera Leaves Against Ovarian Carcinoma Cell Line (OVCAR-3)
Provedor de dados: 52
Autores: Benvenutti,Régis Carlos; Gomes,Denise B.; Zanchet,Barbara; Locateli,Gelvani; Vechia,Cristian Alex Dalla; Zanotelli Serpa,Patrícia; Lutinski,Junir; Rodrigues Junior,Sinval Adalberto; Schönell,Amanda Patrícia; Diel,Kriptsan Abdon Poletto; Miorando,Daniela; Ernetti,Jackeline; Alves,Bianca de Oliveira; Zilli,Gabriela Adriany Lisboa; Banzato,Thais Petrochelli; Ruiz,Ana Lúcia Tasca Gois; Vidal Gutiérrez,Max; Vilegas,Wagner; Roman Junior,Walter Antônio.
Abstract Natural products, especially phytochemicals, have been extensively studies and have exhibited important antiproliferative effects. The American native species Urera baccifera (L.) Gaudich. ex Wedd. (Urticaceae) is widely distributed in Brazil, where it is known as urtiga-vermelha or urtigão. The leaves are popularly used as anti-inflammatory, antirheumatic and in the treatment of gastric disorders. However, the antiproliferative potential of this plant against human tumor cells remain to be elucidated. In this study, we evaluated the antiproliferative effects of U. baccifera leaves extracts and fractions against a panel of human tumor cell lines in vitro besides a chemical evaluation of the most active sample by mass spectrometry (ESI-IT-MSn). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antitumor agents; Medicinal plants; Chemical analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100412

Testis and vas deferens morphology of the red-clawed mangrove tree crab (Goniopsis cruentata) (Latreille, 1803)
Provedor de dados: 52
Autores: Garcia,Tatiane Martins; Silva,José Roberto Feitosa.
The present study describes the structure of the testes and vas deferens of the red-clawed mangrove tree crab, Goniopsis cruentata. Testes and vas deferens were fixed with cold Bouin's solution and submitted to histological routine examinations. Light microscopy showed the testis to be divided into three regions: anterior, containing the germinal zone; intermediary, including the collecting duct; and posterior. The vas deferens, which was continuous with the testes, could be divided into two regions: one corresponding to the expansions and another lined by cubical epithelium, collagenous fibers and a circular layer of muscle cells and containing the androgenic gland. Spermatophores were observed in both vas deferens regions suggesting that they...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachyura; Goniopsis cruentata; Reproduction; Spermatophore; Testis; Vas deferens.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300019

Ferulic acid depletion by cultured soybean seedlings under action of glucose and methionine
Provedor de dados: 52
Autores: Herrig,Vanessa; Silva,Hélio Cândido da; Ferrarese,Maria de Lourdes Lucio; Ferrarese-Filho,Osvaldo.
Cultured soybean seedlings were used to investigate how glucose or methionine influenced depletion of ferulic acid. Three-day-old seedlings were grown in hydroponic solution containing ferulic acid plus glucose or methionine, and the level of the phenolic acid were monitored in the nutrient culture. The results showed that ferulic acid depletion was more rapid in the presence of those compounds. After 6 h, the increase caused by glucose (0.01 and 0.05 mM) was more pronounced than methionine in the same concentrations. On the other hand, methionine (0.1 and 0.2 mM) increased depletion more significantly than glucose. Results suggested that both compounds might to increase the allelopathic effects of ferulic acid in the seedlings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max L. Merr.; Root; Phenolic acid; Carbohydrate; Aminoacid.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500011

Production of Antimicrobial Metabolites by Streptomyces lavendulocolor VHB-9 Isolated from Granite Mines
Provedor de dados: 52
Autores: B.S.S.N,Hima Bindu; Muvva,Vijayalakshmi; Munaganti,Rajesh Kumar; Naragani,Krishna; Konda,Saidulu; Dorigondla,Kumar Reddy.
ABSTRACT The actinobacterial strain Streptomyces lavendulocolor VHB-9 was isolated from granite mine soil samples of Khammam district, Telangana state, India. The strain was identified based on detailed microorphological, cultural and phylogenetic analysis. Bioactive guided isolation of the secondary metabolites of the strain was carried out by growing the strain in optimized medium (0.5% lactose, 0.5% peptone, 0.05% K2HPO4, 0.2% CaCO3 with pH adjusted to 7.0). Separation and purification of the active fractions from the crude ethyl acetate extract was carried out by silica gel column chromatography and resulted in the isolation of two active fractions. Structural elucidation of the two (B2 and B3) active compounds was carried out by FT-IR, Mass and NMR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granite; Streptomyces lavendulocolor; Spectroscopy; Bis (7-methyloctyl) phthalate; (Z)-3-aminoacrylic acid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100413

Bacterial production in Guanabara Bay (Rio de Janeiro, Brazil) evaluated by ³H-leucine incorporation
Provedor de dados: 52
Autores: Gonzalez,Alessandra M.; Paranhos,Rodolfo; Andrade,Luciana; Valentin,Jean L.
The aim of this work was to evaluate the necessary ³H-leucine concentration to estimate bacterial production in Guanabara Bay through saturation curves. A second aim was to collect preliminary data of bacterial production in two distinct sites corresponding to different water qualities: Urca inlet and Governador Island. Saturation curves were made with water samples taken at the main circulation channel of the bay, Paquetá Island, and the two sites mentioned before. The ³H-leucine curves showed similar pattern for all studied areas, indicating the ideal isotope concentration to be 10 nM. Bacterial biomass production ranged from 0.40 to 4.53 µgC L-1 h-1 in Urca and from 3.86 to 73.72 µgC L-1 h-1 in Governador Island indicating the relationship between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterioplankton; Activity measurement; Heterothrophic production; Estuary; Water pollution; Eutrophication.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500008

Anthracycline production by Streptomyces capoamus in batch fermentation
Provedor de dados: 52
Autores: Martins,Cristiane Soares; Souto-Maior,Ana Maria.
Anthracyclines have been intensely studied worldwide due to their importance as anticancer agents. In this work investigation was made on the production of ciclamycin, an antitumour complex of the anthracycline class, produced by Streptomyces capoamus. The fermentation medium consisted of glucose, as the main carbon source, and soymilk, as the sole nitrogen source. Experiments were performed in a 5-liter batch bioreactor under nitrogen and carbon-limiting conditions. Increasing antibiotic activity was observed both intra and extracellularly during growth under the two conditions used. Progressive loss of activity occurred in both cases after glucose was depleted from the medium. The results obtained showed that harvest of ciclamycin in a batch process...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthracycline; Ciclamycin; Streptomyces capoamus; Batch fermentation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300021

Spermatic feasibility after 20 years of freezing and refreezing: case study
Provedor de dados: 52
Autores: Taitson,Paulo Franco; Souza,Maria do Carmo Borges de.
The aim of the present work was to study the spermatic feasibility after 20 years of freezing, thawing and refreezing. A young man affected by testicular cancer sought the sperm freezing service. After 20 years, the samples were thawed and frozen again. After two days, thawing and analysis of the material took place. Comparison of the findings showed activity reduction from the first thawing to the fresh semen 59.09%. For the second thawing it was 77.27%. Considering the morphology, first thawing showed a reduction of 44.64% in relation to fresh semen. In the second thawing, the reduction was of 75%. Spermatic motility decreased by 64.28% in relation to fresh semen and 92.85% at the level of second thawing. The patient had gone through in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technology for freezing semen; Cancer; Semen storage; Sperm banking.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400014

Production biomolecule with inhibitory activity against Gram-negative bacteria isolated from faeces of broilers and swine
Provedor de dados: 52
Autores: Bordignon Junior,Sidnei Emílio; Miyaoka,Mitiyo Fukuda; Spier,Michele Rigon; Rubel,Rosália; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo.
The aim of this study was to investigate the potential of lactic acid bacteria, bacilli and yeasts isolates to produce antimicrobial substances, especially against Gram-negative bacteria isolated from the animal faeces, searching for a new alternative to control the enteritis diseases in animal health. Evaluations were performed by microdilution in broth using crude extract obtained from the cultivation of 272 strains against Escherichia coli, S. Typhimurium, S. Enteritidis and Pseudomonas aeruginosa. Thirty-five lactic acid bacteria presented some antimicrobial action. One Lactobacillus isolate, named FLPB-1, was selected to studies the kinetics of the production. An important increase in the production was obtained when the producer strain had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial; Enteritis; Lactic acid bacteria; Bacteriocin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400011

Spatial distribution and diversity of bird community in an urban area of Southeast Brazil
Provedor de dados: 52
Autores: Manhães,Marco Antônio; Loures-Ribeiro,Alan.
Since the campus of the Federal University of Juiz de Fora, Minas Gerais State, Brazil, has different landscapes, it turns up to be a useful ecological model to evaluate the influence of habitat heterogeneity on bird communities. Our research goals were to know the local avifauna and compare its composition and bird diversity within the different landscapes. Species were identified in point counts without distance estimation, in four habitats: secondary woodlot, lake and surroundings, scrub/abandoned grazing areas and urban areas. One hundred and twenty-one species were identified, but no difference in diversity among the habitats was found. However, analyses indicated the existence of greater similarities among the sampling points belonging to the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bird diversity; Southeast Brazil; Habitat heterogeneity; Urban landscapes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200016

Antimicrobial activity of essential oils from Pimenta pseudocaryophyllus and Tynanthus micranthus
Provedor de dados: 52
Autores: Custódio,Dayana Lacerda; Burgo,Rafaela Pinheiro; Moriel,Bárbara; Barbosa,Aneli de Melo; Rezende,Maria Ines; Daniel,Juliana Feijó de Souza; Pinto,Jurandir Pereira; Bianchini,Edmilson; Faria,Terezinha de Jesus.
The present study describes the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from the leaves of Pimenta pseudocaryophyllus (1.0% w/w) and Tynanthus micranthus (1.1% w/w). GC and GC/MS analysis demonstrated that eugenol was the only component in the T. micranthus essential oil (99.9%) and the major component in the P. pseudocaryophyllus essential oil (92.59%), which also presented methyleugenol, terpinen-4-ol, o-cymene and (E)-caryophyllene, among others. Both the oils presented antimicrobial activity against bacteria, yeast and filamentous fungi tested.The Bioautography test revealed that eugenol was the bioactive component in both the oils against Cladosporium herbarum. This is the first report about the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pimenta pseudocaryophyllus; Tynanthus micranthus; Essential oil; Antimicrobial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600013

Evaluation of cell proliferation and apoptosis in placentas of rats with severe diabetes
Provedor de dados: 52
Autores: Rudge,Marilza Vieira Cunha; Costa,Elaine; Barbisan,Luis Fernando; Damasceno,Débora Cristina; Bueno,Aline; Saito,Felipe Hiroshi; Calderon,Iracema Mattos Paranhos; Rodrigues,Marcela Marcondes Pinto.
The aim of this work was to analyze the cell proliferation and apoptosis indexes on the 18th and 21st days of pregnancy of diabetic rats and to correlate with maternal glycemia and perinatal outcomes. Placentas from 20 Wistar rats were collected and divided into four experimental groups: control and diabetic of 18 and 21 days of pregnancy. The cell proliferation was analyzed using the PCNA expression and apoptosis by the TUNEL method. It was observed that PCNA and TUNEL indexes decreased from day 18 to 21 of pregnancy in the placentas of diabetic rats and these values were lower than control groups. Diabetic dams presented higher percentage of small for pregnancy age (SPA) fetuses. However, there was no difference between the PCNA and TUNEL indexes in SPA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Cell proliferation; Apoptosis; Placenta; Rat; IUGR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200009

Influence of genotype on protein and oil concentration of soybean seeds
Provedor de dados: 52
Autores: Santos,Esmael Lopes dos; Pípolo,Antonio Eduardo; Faria,Ricardo Tadeu de; Prete,Cássio Egidio Cavenaghi.
Aiming at evaluating genotype influence on the concentration of protein and oil, immature seeds of cultivars CD 202 and CD 206 were removed from the mother-plant, in the stage R5, and were grown in vitro, in a liquid culture medium which contained 20, 40 and 60 mM of glutamine, during eight days. Afterwards, the concentrations of oil and protein were compared to the contents of the seeds cultivated in vivo. With a higher availability of glutamine for the seed, there was an increase of protein content. The genotypes were statistically different as far as the protein concentration was concerned,which confirmed that the genotype had influence on the concentration of protein in the seed. Oil and protein concentrations were inversely related when a variation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamine; Fresh weight; Seed composition; In vitro growth and Glycine max.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400007

In vitro Callus Induction and Plant Regeneration of Celosia argentea- An Important Medicinal Plant
Provedor de dados: 52
Autores: Bakar,Dalilah Abu; Ahmed,Bakrudeen Ali; Taha,Rosna Mat.
Celosia argentea (Var.) cristata (Amaranthaceae) is a widely cultivated ornamental plant, which has antibacterial, astringent, haemostatic, hypertensive, ophthalmic, and parasitic significance. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of C. argentea using Murashige and Skoog (MS) medium. Callus culture was initiated and established from seedling, leaf, and stem explants. Explants were cultured on MS medium supplemented with auxin alone (0.5 mg/L Naphthaleneacetic acid (NAA), 2, 4-Dicholorophenoxyacetic acid (2, 4-D). Green and red compact callus (98%) were induced using MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L Benzyladenine (BA). Two different concentrations (1.0 mg/L BA +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celosia argentea; Amaranthaceae; In vitro; Micropropagation; Callus; Plant regeneration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600860

Ananalysis on Runtime Associated QOS-Based Proficient Web Services Discovery Optimization
Provedor de dados: 52
Autores: Amirthasaravanan,A.; Rodrigues,Paul; Sudhesh,R.
ABSTRACT In today's web world, Service-oriented architectures represent the main standard for IT infrastructures. Certainly, with the initiation of service oriented architecture, Web services have gained incredible growth. Web service discovery has become increasingly more significant as the existing use of web service. Discovering most appropriate web service from vast collection of web services is very decisive for successful execution of applications. In automation of web service discovery, there is always a need to deem Quality of Service (QoS) attributes during matching. A study of literature concerning the evolution of different web service discovery optimization methods with unique prominence to quality motivated service discovery have been carried...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Web Services; Service-Oriented Architectures; Discovery; Quality of Service; Bio-Inspired Algorithm; Metaheuristics; Optimization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300601

Qualitative and quantitative analysis of environmental damages through instauration and registers of lawful documents
Provedor de dados: 52
Autores: Cintra,Roberta Hehl de Sylos; Santos,José Eduardo dos; Moschini,Luiz Eduardo; Pires,José Salatiel Rodrigues; Henke-Oliveira,Carlos.
A qualitative and quantitative analysis of the environmental damages occurrences was conducted through the lawful documents established for charging at the State Prosecution Service of São Carlos city, State of São Paulo, during 1991 - 2000, to understand the relationship between environmental problems and the current development actions. The environmental damage identified were grouped into nine classes, where deforestation was prevailing in each year along the period of study. Of the total number of deforestation occurrences, 180 was related to areas smaller than 5 ha. It corresponded to 110.35 ha of the total deforested area. The other 37 were associated with areas higher than 5 ha, equivalent to 1,013.27 ha. The registers of environmental damages in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental damages; Deforestation; Lawful documents.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700017

Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
Provedor de dados: 52
Autores: Packiam,Soosaimanickam Maria; Emmanuel,Cyril; Baskar,Kathirvelu; Ignacimuthu,Savarimuthu.
Newly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20 ppm. Among various phytopesticidal formulations, neem and pongam oils at 1:1 ratio, called PONNEEM showed significant feeding deterrent activity against H. armigera at 20 ppm concentration and wasgenotoxic to H. armigera (P&gt;0.001). The comet parameters, namely tail moment (arbitrary units), tail length (µm) and tail DNA (%) were observed at all the concentrations of PONNEEM. Statistically significant changes in all the comet parameters of H. armigera were observed at 20 ppm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; PONNEEM; Feeding deterrent; Helicoverpa armigera.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400487

Multi-criteria Selection of Distributed Mini Generation Systems Using Rice Husk
Provedor de dados: 52
Autores: Costa,Alexandre Kunkel da; Farret,Felix Alberto; Trennepohl,Felipe Alex.
ABSTRACT This paper presents a multi-criteria methodology to support decision making for management and selection of distributed mini generation sources (GD) using rice husk. Considering the potential of residual biomass, the developed model is based on the Analytic Hierarchy Process (AHP) method to evaluate the main technological arrangements of generation against the technical, economical, social-environmental aspects. Considering the possibility of energy transformation of rice husk, the following alternatives for distributed mini generation are considered: steam turbine, gas turbine, micro turbine, fuel cells, alternative combustion engine and Stirling engine. Regarding the evaluation aspects, it is defined energy efficiency, environmental impacts,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass; Rice husk; Distributed mini generation; Multi-criteria selection; AHP.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200201

Multiple gene sequence analysis using genes of the bacterial DNA repair pathway
Provedor de dados: 52
Autores: Rotelok Neto,Miguel; Galvão,Carolina Weigert; Cruz,Leonardo Magalhães; Guizelini,Dieval; Silva,Leilane Caline; Garcia,Jarem Raul; Etto,Rafael Mazer.
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways. Despite the amount of available genes in public database, the usual method to place genomes in a taxonomic context is based mainly on the 16S rRNA or housekeeping genes. Thus, the relationships among genomes remain poorly understood. In this work, an approach of multiple gene sequence analysis based on genes of DNA repair pathway was used to compare bacterial genomes. Housekeeping and DNA repair genes were searched in 872 completely sequenced bacterial genomes. Seven DNA repair and housekeeping genes from distinct metabolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial genome; Multiple gene sequence analysis; Taxonomic assignment; DNA repair genes; HGT.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300421

Occurrence and parasitism of aphids (Hemiptera: Aphididae) on cultivars of irrigated oat (Avena spp.) in São Carlos, Brazil
Provedor de dados: 52
Autores: Ronquim,Júlio Cesar; Pacheco,Josué Marques; Ronquim,Carlos Cesar.
The interactions between aphids and their Hymenopteran parasitoids on irrigated oats as well as the response of different cultivars of cereals regarding the resistance to these aphids and the influence on the host/parasitoid relationships were studied during two years in São Carlos, Brazil. Rhopalosiphum padi (L.) was the predominant aphid observed throughout the study, while the other species were rarely found. Five species of parasitic Hymenoptera were found: three primary parasitoids, Lysiphlebus testaceipes (Cresson), Aphidius colemani (Viereck) and Diaeretiella rapae (M'Intosh) and two hyperparasitoids, Syrphophagus aphidivorus (Myer) and Alloxysta brassicae (Ashmead). The UPF 86081 cultivar presented significant results regarding lower Rhopalosiphum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aphids; Biological control; Oats; Parasitoid; Plant resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200001

Use of Different Kinds of Solutes Alternative to Sucrose in Osmotic Dehydration of Yacon
Provedor de dados: 52
Autores: Brochier,Bethania; Marczak,Ligia Damasceno Ferreira; Noreña,Caciano Pelayo Zapata.
The present work aimed to evaluate glycerol, maltodextrin, polydextrose and sorbitol for the osmotic dehydration of yacon for diabetics, keeping its properties as prebiotic. Osmotic dehydration was carried out using a yacon to 33% concentrated syrup weight ratio of 1:12, with magnetic stirring at 23ºC and atmospheric pressure. The best results were achieved for glycerol and sorbitol with 80 ± 4% and 81± 1% of water removal and increase of 3.73 ± 0.11 and 4.30 ± 0.16 times in total soluble solids respectively. Maltodextrin did not promote dehydration.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osmotic dehydration; Osmotic agent; Yacon; FOS; Inulin; Prebiotic.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100034

Morphology of gonads, maturity and spawning season of Loricariichthys spixii (Siluriformes, Loricariidae) in a Subtropical Reservoir
Provedor de dados: 52
Autores: Duarte,Silvana; Araújo,Francisco Gerson; Sales,Armando; Bazzoli,Nilo.
The gonad morphology and spawning season of Loricariichthys spixii in Lajes reservoir were described based on 175 males and 613 females. Cells from the spermatogenic lineage were divided in four phases: spermatogonia (primary and secondary), spermatocytes (primary and secondary), spermatids and spermatozoa, and the cells from the ovocitarian lineage were divided in four phases: primary oocytes (O1), previtellogenic oocytes (O2), cortical vesicle oocytes (O3) and yolk globules or vitellogenic (O4). Five gonadal stages were described for the males/females according to oocytes and spermatogenic lineage cells distribution: resting (1); initial maturation (2a); advanced maturation (2b); partially spent/spawned (4a); totally spent/spawned (4b). Spawning was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loricariidae; Reservoir; Fish reproduction; Gonad morphology; Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700013

Apoptosis induction by E2F decoy DNA of the prostate cancer cell line
Provedor de dados: 52
Autores: Wang,Tao; Jiang,An -li; Zhang,Jian-ye; Jing,Fei.
To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decoy DNA; Transcription factors; PC-3M cells; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200011

Functional properties of PSE (Pale, Soft, Exudative) broiler meat in the production of mortadella
Provedor de dados: 52
Autores: Kissel,Cassiana; Soares,Adriana Lourenço; Rossa,Alessandro; Shimokomaki,Massami.
This work was carried out in order to evaluate whether the functional properties of broiler meat are affected by the factors that lead to PSE (Pale, Soft, Exudative). PSE meat was characterized by pH and L* values, and mortadella formulations consisted of isolated soy protein, sodium tripolyphosphate, and cassava starch in addition to PSE and normal meats. The functionality of the meat was evaluated by examining the water holding capacity (WHC), texture profile, emulsion stability (ES) and color of the final products. The results show that in mortadella prepared with PSE meat, the protein denaturation affected the ES. Additives are necessary to enhance the functional properties of PSE meat.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mortadella; Water holding capacity; Texture profile; Meat color; Emulsion stability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700027

Habitat selection by fish in an artificial reef in Ilha Grande Bay, Brazil
Provedor de dados: 52
Autores: Brotto,Daniel Shimada; Araujo,Francisco Gerson.
The use of artificial structures as a shelter for fishes was evaluated aiming to assess increase in fish abundance in Ilha Grande Bay at Southeast Brazilian coast (Lat: 22°8'-23°5' S; Long. 44º05’-44º40’W). Five different types of structures, made of clay tiles, with an increase range on size and complexity were tested to assess their efficiency. Fish were counted in each type of structure from May-1996 to April-1997 by underwater observation made with snorkelling. According to the pattern of concentration in the structures, two groups of fishes were identified. One comprised by E. guaza, M. rubra, R. randalli, S. hispidus, Sphoeroides sp and Haemulun sp showed affinity for hard bottom, irregular profile and an increase in abundance on the more complex and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial Reefs; Brazil; Fish; Habitat selection.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300015

Application of RAPD-PCR for Determining the Clonality of Methicillin Resistant Staphylococcus aureus Isolated from Different Hospitals
Provedor de dados: 52
Autores: Idil,Neslihan; Bilkay,Isil Seyis.
Randomly amplified polymorphic DNA (RAPD)-PCR was applied with ten random 10-mer primers to examine the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) strains in the hospitals and to investigate the epidemiological spread of these strains between different hospitals. The main objective of the study was to identify appropriate primers, which successfully established the clonality of MRSA. Three of the primers yielded particularly discriminatory patterns and they were used to perform the RAPD analysis which revealed different bands ranging from 200 to 1500 bp. Dendogram was created by the un-weighted pair group method using arithmetic (UPGMA) average clustering and it was constructed based on the combination results of the new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; RAPD-PCR; Clonal dissemination; UPGMA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400548

Molecular weight estimation of esterase isoenzymes in closely related Drosophila Species (Diptera: Drosophilidae) in non-denaturing polyacrylamide gel electrophoresis
Provedor de dados: 52
Autores: Mateus,Rogério Pincela; Cabral,Hamilton; Bonilla-Rodriguez,Gustavo Orlando; Ceron,Carlos Roberto.
A method that allows the measure of molecular weight of two well-known and closely related esterases from Drosophila mojavensis and its sibling species, D. arizonae, is here described, using native polyacrylamide gel electrophoresis at several concentrations, applying Fergunson´s principles. These enzymes, namely EST-4 and EST-5, presented molecular weight values between 81 and 91 kDa. In spite of their distinct expression pattern through the insect's life cycle, they showed properties of isoenzymes codified by distinct structural genes, supporting the hypothesis of a rather recent gene duplication event that generated both in D. mojavensis and D. arizonae, as well as in other species of repleta group. The method is simple and adequate to be applied to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esterases; Isoenzymes; Molecular weight determination; Drosophila mojavensis; Drosophila arizonae; PAGE.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500004

Efficiency of Single Site Phase Transfer Catalyst in Free Radical Polymerization of Butyl Methacrylate - A Kinetic Study
Provedor de dados: 52
Autores: M,Meena; M. J.,Umapathy.
ABSTRACT The effectiveness of single site phase transfer catalyst- 2- benzoylethyldecyldimethylammonium bromide has been studied with the help of kinetics of free radical polymerization of butyl methacrylate. The radical polymerization was catalysed with single site phase transfer catalyst, initiated by water soluble potassium peroxydisulphate initiator in ethyl acetate / water biphasic media, under inert and unstirred condition at constant temperature, 60±1ºC. The dependence of rate of polymerization on various experimental conditions, different concentrations of monomer, initiator, and phase transfer catalyst was evaluated and effect of temperature as well as solvent polarity was determined. The order of the reaction with respect to monomer, initiator...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single sitephase transfer catalyst 2- Benzoylethyldecyldimethylammonium bromide; Kinetic mechanism Radical polymerization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300604

Effects of low-dose ionising radiation on pituitary adenoma: is there a role for L-type calcium channel?
Provedor de dados: 52
Autores: Soares,Marcella Araugio; Santos,Raquel Gouvêa dos.
Pituitary adenomas constitute about 6-18% of brain tumours in adults. Activation of voltage gated calcium currents can account for growth hormone oversecretion in some GH-secreting pituitary adenomas that produce an acromegaly appearance and increase mortality. Ca2+ ions, as mediators of intracellular signalling, are crucial for the development of apoptosis. However, the role of [Ca2+] in the development of apoptosis is ambiguous. In this study, the effects of low-dose ionising gamma radiation (60Co) on rat pituitary adenoma cells survival and proliferation and the role of calcium channels on the apoptosis radio-induced were evaluated. Doses as low as 3 Gy were found to inhibit GH3 cell proliferation. Even though there was a significant number of live...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium channels; Ionising radiation; Pituitary adenoma cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700028

Computational Verification of the NBR 15.220-3 Recommendations for Thermal Comfort in the City of Curitiba
Provedor de dados: 52
Autores: Martins,Mariana Ribeiro; Schmid,Aloísio.
Abstract The Brazilian Standard for Thermal Performance and Bioclimatic Zoning (NBR 15.220-3) establishes twelve strategies to achieve thermal comfort inside buildings considering the dry bulb temperature and the predominant humidity in each climate. These strategies are visualized in the Bioclimatic Charts of Brazil main cities. This study seeks, through computational simulation by the EnergyPlus software, to test the bioclimatic strategy proposed by the Standard for the city of Curitiba through the addition of thermal mass. The goal is to check the validity of the temperature limits related to the constructive guidelines described. The article is restricted to the analysis of Bioclimatic Zone 01 in which Curitiba is inserted. To this end, the unit of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioclimatic Chart; NBR 15.220-3; Thermal Mass Zone; Thermal performance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200212

Biodegradation and toxicological evaluation of lubricant oils
Provedor de dados: 52
Autores: Tamada,Ivo Shodji; Lopes,Paulo Renato Matos; Montagnolli,Renato Nallin; Bidoia,Ederio Dino.
The aim of this work was to compare different toxicity levels of lubricant oils. The tests were performed using the earthworm (Eisenia andrei), arugula seeds (Eruca sativa) and lettuce seeds (Lactuca sativa), with three types of contaminants (mineral lubricant oil, synthetic lubricant oil and used lubricant oil) for various biodegradation periods in the soil. The toxicity tests indirectly measured the biodegradation of the contaminants. The samples were analyzed at t0, t60, t120 and t180 days of biodegradation. The used lubricant oil was proved very toxic in all the tests and even after biodegradation its toxicity was high. The mineral and synthetic oils were biodegraded efficiently in the soil although their toxicity did not disappear completely after 180...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxicology; Biodegradation; Lubricant oil; Toxicity bioassay.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600020

Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
Provedor de dados: 52
Autores: Oliveira,Gilmara Alvarenga Fachardo; Pádua,Juliano Gomes; Costa,Juliana Leles; Jesus,Onildo Nunes de; Carvalho,Fabiana Moraes de; Oliveira,Eder Jorge de.
The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transferability; SSR; Passion fruit.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009

Floristic survey of the herbaceous-shrub layer of a gallery forest in Alto Paraíso de Goiás - GO, Brazil
Provedor de dados: 52
Autores: Chaves,Eduardo; Soares-Silva,Lucia Helena.
Flowering species of the herbaceous and shrubby layer were sampled in a gallery forest at Portal da Chapada, Alto Paraíso de Goiás-GO in the Chapada dos Veadeiros during 12 months growing within 4.5 meters on either side of a raised pathway 2.4 Km long. The collections were included to University of Brasilia Herbarium (UB). A total of 138 species (103 genera and 40 families) were recorded; the most species-rich families were Asteraceae (22), Poaceae (14), Rubiaceae (12), Fabaceae (11) and Melastomataceae (7). The richest family in number of genera was Asteraceae (19), followed by Poaceae (8), Fabaceae (8) and Rubiaceae (7). The results showed a meaningful increase in the Chapada dos Veadeiros flora, especially considering the herbaceous-shrubby layer of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Floristic survey; Cerrado; Chapada dos Veadeiros.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500011

Biology of Chirocentrodon bleekerianus (Poey, 1867) (Clupeiformes: Pristigasteridae) in a continental shelf region of southern Brazil
Provedor de dados: 52
Autores: Corrêa,Carlos Eduardo; Chaves,Paulo de Tarso; Guimarães,Paulo Ricardo Bittencourt.
This study provides information on the biology of C. bleekerianus from Paraná State coast, Brazil, assembling data about its abundance, size structure, diet and reproduction. Monthly collections were accomplished in 1999 and 2000 in the internal continental shelf, with trawl net. The catch-per-unit-effort did not vary significantly among the seasons. In the summer, the individuals were larger, followed by autumn, spring and winter. C. bleekerianus was predominantly planktivorous and the most frequent items in its diet were copepods followed by diatomaceous algae. Seasonal variation in the frequency values of the gonad maturation stages, gonadossomatic index and gonadal condition factor indicated that the reproductive period encompassed spring and summer....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abundance; Brazil; Chirocentrodon; Clupeiforms; Feeding; Reproduction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300013

Diallel among soybean genotypes with high oil content and resistance to sudden death syndrome
Provedor de dados: 52
Autores: Oliveira,Inocencio Junior de; Vello,Natal Antonio; Vieira,Paulo de Melo Jorge; Wyminersky,Philip Traldi.
The objective of this work was to use a partial diallel to evaluate the combining ability of 14 soybean genotypes and recommend superior combinations. Seven of the genotypes had high oil yield; other seven exhibited tolerance to sudden death syndrome and they generated 49 hybrids in a diallel cross without reciprocals. F2plants of each cross and the parents were evaluated for the following traits: number of days to maturity (NDM), oil content in the seeds (OC), grain yield (GY) and oil yield (OY). The results indicated the existence of genetic variability in the parents and progeny for all the traits. The rank of the parents based on the means was similar to the rank based on general combining ability (GCA) for all the traits. The specific combining...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Combining ability; Oil yield; Tolerance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200004

Effect of Thymol on Soybean Seeds Germination: Physiological and Biochemical Analysis
Provedor de dados: 52
Autores: Lima,Eduarda Lee Ferreira; Macedo,Willian Rodrigues; Silva,Geraldo Humberto.
Abstract Higher levels of reactive species of oxygen are harmful to plant tissues. This study evaluated the action of different doses of thymol on soybean seed germination, biometric analysis and enzymatic parameters; both involved in germination process. High doses of thymol affected the plantlet growth, but not hampered the germination.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbate peroxidase; Catalase; Superoxide dismutase; Glycine max L; Seed vigor.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100211

Glycogen levels and energy status of the liver of fasting rats with diabetes types 1 and 2
Provedor de dados: 52
Autores: Oliveira,Denise Silva de; Amado,Ciomar Aparecida Bersani; Martini,Mirian Carvalho; Suzuki-Kemmelmeier,Fumie; Bracht,Adelar.
Glycogen levels and the energy status of livers from fasting rats with diabetes types 1 and 2 were measured. After a 24 h fast, the hepatic glycogen levels of rats with diabetes1 and diabetes2 were, 18.7 and 2.6 times higher, respectively, than those of livers from the normal rats. In diabetes1 rats, the glycogen levels decreased when the fasting period was extended to 48 and 72 h. The opposite occurred with the control and diabetes2 rats. Consistently, glucose release by the perfused livers from diabetes1 rats was considerably higher during at least 60 minutes after initiating perfusion. The hepatic ATP content of diabetes1 rats was similar to that of the control rats; in diabetes2 rats, the hepatic ATP content was increased. It could be concluded that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes type 1; Diabetes type 2; Rats; Hepatic glycogen; Hepatic energy status.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500006

Isolation and Identification of Cellulolytic Bacteria from the Gut of Three Phytophagus Insect Species
Provedor de dados: 52
Autores: Shil,Rajib Kumar; Mojumder,Suman; Sadida,Faozia Faleha; Uddin,Myn; Sikdar,Dwaipayan.
The cellulolytic bacteria from the gut of three different phytophagous insects were studied to isolate novel cellulolytic organism for biofuel industry. Among the threse, gut of P. quatuordecimpunctata larvae contained both highest no of total bacterial count (6.8x107CFU/gut) and cellulolytic bacteria (5.42x103CFU/gut). Fifteen different isolates were obtained from the gut of O. velox, A. miliarisand P. quatuordecimpunctata. All the isolates produced clear zone in CMC medium staining with Congo red. The isolates included Gram positive Enterococcus, Microbacterium and Gram negative Aeromonas, Erwinia, Serretia, Flavobacterium, Acenitobacter, Klebsiella, Yersinia, Xenorhabdus, Psedomonas and Photorhabdus. Out of the fifteen isolated and identified bacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gut bactéria; Cellulolytic; O. velox; A. miliaris; P. quatuordecimpunctata.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600927

Effects of the infrared lamp illumination during the process of muscle fatigue in rats
Provedor de dados: 52
Autores: Abou-Hala,Andréia Zarzour; Barbosa,Daniella Galvão; Marcos,Rodrigo Labat; Pacheco-Soares,Cristina; Silva,Newton Soares da.
In this study the effects of infrared lamp illumination during the muscle fatigue process was studied. Three different groups (n=5) were used: one control group and two treated (Infrared Lamp 780-1400nm), with the energy densities of 0.5 and 1.0 J/cm² and time of illumination of 300 seconds. The treated animals were illuminated in one point directly in the tibialis muscle, after the first tetanic contraction out of six, with an interval between each tetany. The results were registered in an electrophysiograph and the intensity of the force of contraction in grams was analysed. It was observed that the control group presented a reduction in the intensity of the force of contraction, while the treated group managed to maintain it, which was clearly evident...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle fatigue; Infrared illumination; Muscle tension; Tetany.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300006

New aminoporphyrins bearing urea derivative substituents: synthesis, characterization, antibacterial and antifungal activity
Provedor de dados: 52
Autores: Karimipour,Gholamreza; Kowkabi,Saeed; Naghiha,Asghar.
This work studied the synthesis of 5,10,15-tris(4-aminophenyl)-20-(N,N-dialkyl/diaryl-N-phenylurea) porphyrins (P1-P4 with alkyl or aryl groups of Ph, iPr, Et and Me, respectively) and also the preparation of their manganese (III) and cobalt (II) complexes (MnP and CoP). The P1-P4 ligands were characterized by different spectroscopic techniques (1H NMR, FTIR, UV-Vis) and elemental analysis, and metalated with Mn and Co acetate salts. The antibacterial and antifungal activities of these compounds in vitro were investigated by agar-disc diffusion method against Escherichia coli (-), Pseudomonas aeruginosa (-), Staphylococcus aureus(+), Bacillus subtilis (+) and Aspergillus oryzae and Candida albicans. Results showed that antibacterial and antifungal activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Porphyrin; Aminoporphyrins; Antibacterial/Antifungal Activity; Synthesis; Carbamoyl Chloride.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300431

Fauna Parasitológica Paranaense: IV. Lista Prévia da Ocorrência de Helmintos em Animais Domésticos
Provedor de dados: 52
Autores: Giovannoni,Milton; Kubiak,Gastão V. L.
The authors present a list of helmints found in 161 necropsis, performed in the Instituto de Biologia e Pesquisas Tecnológicas, employing animals relieved from other services. In a table, are assembled the data in groups, used to call the attention of the technicians from the Animal Sanitary Defense for their observation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helmints; Domestic animals; Animal parasitology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500011

Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil
Provedor de dados: 52
Autores: Nwagu,Tochukwu Nwamaka; Okolo,Bartholomew Ndubuisi.
In this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC. Soybean meal at 1% concentration, supplemented with 0.2% NH4Cl and 2.5% corn starch elicited the highest amylase yield. Optimum pH for the enzyme was pH 6.5 which retained over 60% of its activity after 24 h incubation at the pH range of 4.5-7.0. The enzyme showed high activity from 40-70 0C with optimal activity at 50 ºC and 78% activity was retained after incubation at 70 ºC for 30min. Catalytic function of the crude amylase was stimulated by Mg (136%), Ca (118%) and Zn (118%) at 2mM concentrations. The enzyme hydrolyzed cassava, potato and yam starches effectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrolysis; Starch; Fungal growth; Amylase synthesis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002

State of the Art of the Application of Energy Storage Systems in Photovoltaic Buildings
Provedor de dados: 52
Autores: Mariano,Juliana D’Angela; Urbanetz Junior,Jair.
Abstract Photovoltaic (PV) buildings are increasingly present in urban centers and can generate their own energy becoming independent of the grid, depending on their consumption profile. However, most residential and commercial consumers show their peak demand at night, when there is no photovoltaic generation, needing the electricity grid to meet the demand of these facilities. Peak demand lead to increased costs for these consumers and end up disrupting the power quality of the grid. One possible solution for these listed problems is by applying storage systems to these buildings, which is already being done in some countries and can increase the PV generation. Aiming to seek the state of the art of these systems, this article brings a review of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic Buildings; Energy Storage; Peak Demand Reduction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200302

Erratum: Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300900

Escherichia coli in the surface waters and in oysters of two cultivations of Guaratuba Bay - Paraná - Brazil
Provedor de dados: 52
Autores: Forcelini,Helenita Catharina Dalla-Lana; Kolm,Hedda Elisabeth; Absher,Theresinha Monteiro.
The present work aimed to evaluate the contamination of Escherichia coli in the surface waters and oysters from two cultivations of Guaratuba Bay and to analyze the correlation patterns among the concentrations of E. coli in the waters and in the oysters with the local physical-chemical parameters. Samples were collected in the spring of 2007 and summer, autumn and winter of 2008 from two points of the bay (internal point and external point). From each cultivation and sampling period, 18 oysters were collected. The samples of surface water were collected for the measurement of physical-chemical parameters (pH, salinity, temperature, dissolved oxygen, seston, particulate organic matter) and quantification of E. coli. The surface water analyzed in the summer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oysters; Escherichia coli; Guaratuba Bay.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200018

Molluscicidal activity of crown of Christ (Euphorbia splendens var. hislopii) (Euphorbiaceae) latex submitted to pH variation
Provedor de dados: 52
Autores: Vasconcellos,Maurício Carvalho; Santos,José Augusto Albuquerque dos; Silva,Ivonise Paz da; Lopes,Fátima Eliana Ferreira; Schall,Virgínia Torres.
Laboratory and field bioassays have confirmed the specificity of the molluscicidal activity of the Euphorbia splendens var. hislopii latex (crown of Christ) (Euphorbiaceae) over snails of the species Biomphalaria glabrata, B. tenagophila, B. straminea, B. pfeifferi and Bulinus sp. in the control of Schistosoma mansoni. In the present study, the effect of the pH variation on lethal concentration (LC90) over B. tenagophila was evaluated. Bioassays with the aqueous solutions of the latex ranging from 0.4 to 12 µl/l were adjusted for pH of 5.0; 6.0; 7.0 and 8.0, and tested in accordance with methods standardized by World Health Organization. The results obtained indicated that the minor concentration of the latex occurred at pH 6.0 (LC90 = 3.2 µl/l) and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia splendens var. hislopii; Molluscicidal activity; PH variation; Biomphalaria sp.; Bulinus sp.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300013

Effect of adding macro and micro minerals in pig feces fed diets with different levels of probiotic
Provedor de dados: 52
Autores: Huaynate,Rizal Alcides Robles; Thomaz,Maria Cristina; Kronka,Rodolfo Nascimento; Fraga,Alessandro Luís; Scandolera,Antônio João; Budiño,Fábio Enrique Lemos.
This study aimed to verify the effect of diets with different doses (0, 100, 200 and 300 ppm) of probiotic on daily fecal production (DFP) and level of macro and micro minerals in feces of nursing (1st and 2nd initial phases) and growing pigs and during total period. The DFP, as percentage of body weight, was different (P&lt;0.05) among treatments only at 1st initial phase. Fecal levels of macro and micro minerals were similar (P&gt;0.05) among treatments, with exception of calcium and sodium excretion at 1st initial phase and calcium, zinc and nickel during total period. The addition of a poliprobiotic to diets do not affect fecal production of nursing and growing pigs. However, the presence of microorganisms, added (levels of 200 and 300 ppm) in diets...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Digestion; Lactobacillus; Piglet; Zinc.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400005

Use of modified atmosphere in seafood preservation
Provedor de dados: 52
Autores: Soccol,Marcilene C. Heidmann; Oetterer,Marília.
Fish production has increased in Brazil, mainly the fresh-water aquaculture. However, refrigerated fish presents a relatively short shelf-life (approximately 12 days). In view of the increasing demand for fresh products, there is the need of shelf-life lengthening through a combination of methods in order to minimally expose the product to adverse conditions. The use of the modified atmosphere packaging presents the following advantages: lengthening of the products' shelf-life by 50 to 400%, reduction of economic losses, cost reductions by distributing the product over great distances with the need of fewer shipments and the supply of a better quality product. The purpose of this work was to review and discuss the major effects of the modified-atmosphere...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seafood; Freshwater fish; Aquaculture; Modified atmosphere; Vacuum packaging; Shelf-life.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400011

Construção de um micrótomo de congelamento a partir da adaptação de um micrótomo de parafina
Provedor de dados: 52
Autores: Mello,Cesar de; Silva,L.; Zaia,D. A. M.; Zaia,Cassia Thais B. V.
A microtome for frozen sections was developed using the facilities and equipment available in our country. The requirements for making it were: 1) fast frozen of the tissue, 2) easy histological procedure, 3) no necessity of long period for preparation of the tissue for histological procedure, and 4) low cost for making. This study shows results obtained by using the microtome for frozen sections of brain of rats.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cortes de congelamento; Microtomia; Micrótomo de congelamento.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200013

Peroxidase and lipid peroxidation of soybean roots in response to p-coumaric and p-hydroxybenzoic acids
Provedor de dados: 52
Autores: Doblinski,Patrícia Minatovicz F.; Ferrarese,Maria de Lourdes L.; Huber,Domitila A.; Scapim,Carlos Alberto; Braccini,Alessandro de Lucca e; Ferrarese-Filho,Osvaldo.
The scope of the present study was to investigate how the p-coumaric (p-CA) and p-hydroxybenzoic (p-HD) acids affect the peroxidase (POD, EC 1.11.1.7) activity, the lipid peroxidation (LP) and the root growth of soybean (Glycine max (L.) Merr.). Three-day-old seedlings were cultivated in nutrient solution containing p-CA or p-HD (0.1 to 1 mM) for 48 h. After uptake, both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) while increased soluble POD activity, cell wall (CW)-bound POD activity (with 1 mM p-CA and 0.5 mM p-HD) and LP.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max (L.) Merr.; Roots; Phenolic acid.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200009

Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity
Provedor de dados: 52
Autores: Warda,E. Ashour; Abeer,A. Abd El Aty; Eman,R. Hamed; Mahmoud,A. Swelim; Ahmed,I. El-Diwany.
ABSTRACT Biological control strategy which can damage chitin, a vital component of pathogenic fungi and arthropods promises a safe solution for many fungal problems. And it’s more favorable than chemicals which increase health risks and environmental problems. Thus, the chitinase producers appear potential candidates of biological control of pathogenic fungi. Brevundimonus diminuta KT277492 is a new isolate that has been isolated recently from Egyptian soil. Significant factors that affecting the chitinase enzyme production were studied and optimized using Plackett-Burman and Response Surface Methodology (RSM). As a result, maximum production of chitinase enzyme was 832.87 IUL-1, this result presented about 8.767-fold increase in the enzyme production. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitinase; Brevundimonas diminuta KT277492; Optimization; Plackett–Burman; Central composite design; Antifungal.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100429

GC-MS analysis of Nigella sativa seeds and antimicrobial activity of its volatile oil
Provedor de dados: 52
Autores: Gerige,Saptha Jyothi; Gerige,Mahesh Kumar Yadav; Rao,Muralidhara; Ramanjaneyulu,.
The present study dealt with the hydro distillation of Nigella sativa seeds and GC-MS analysis. The total composition of the oil was 86.7%. The seed volatile oil was tested against 19 microbes (Gram positive, Gram negative and fungi), which showed that Gram negative bacteria Haemophilus influenza, Klebsiella pnemoniae, and Proteus vulgaris were highly sensitive against the antimicrobial agent, whereas the fungi such as Trichoderma vibriae,. Pencillium rubrum; and Dermatophyte: Trichophyton mentagrophytes; had no response at the 20µl concentration.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nigella sativa; Volatile oil; Antimicrobial activity; Antifungal activity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500016

Diversity of Plant Species in The Steel City of Odisha, India: Ethnobotany and Implications for Conservation of Urban Bio-Resources
Provedor de dados: 52
Autores: Kumar,Sanjeet; Das,Gitishree; Shin,Han-Seung; Kumar,Pradeep; Patra,Jayanta Kumar.
ABSTRACT The vegetation of the Steel City (Rourkela) of Odisha, India has high ethnobotanical values by virtue of its rich floral diversity. People in the urban area are highly dependent on the plants available in and around the city for their primary needs. The present study highlights the use of local flora, explore, identification, ethnobotany and conservation of wild and cultivated plant species in the city of Rourkela, Odisha, India. It also pays heed to the proper utilization of urban flora as a way of highlighting its ethnopharmacological importance. A field survey was conducted to collect information about floral diversity in and around the city. Data on the use of plants was collected with a semi-structured questionnaire and from the peer-reviewed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Ethnobotany; Ethnopharmacology; Floral diversity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100700

Commercial preslaughter blue light ambience for controlling broiler stress and meat qualities
Provedor de dados: 52
Autores: Barbosa,Claudia Freitas; Carvalho,Rafael Humberto de; Rossa,Alessandro; Soares,Adriana Lourenço; Coró,Fábio Augusto Garcia; Shimokomaki,Massami; Ida,Elza Iouko.
The objective of this work was to study the effect of blue light diffuser on the broiler stress control by measuring the occurrence of PSE meat just before slaughtering. Birds were divided into the following two groups before slaughter at the point of being hung on shackles: broiler group under low intensity blue light ambience (475, 17-20 lx) and control group under white light (550-650nm, 321-332 lx). Birds' stressful conditions were measured by the occurrence of PSE meat. Breast fillets were classified as PSE meat based on pH (&lt;5.8) and lightness (L*&amp;gt; 53.0). The fillet samples in the control group had the following characteristics: pHu=5.77, L* = 54.26 and b*= 6.27. The fillet samples from birds under blue light ambience had the following...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Color; Preslaughter handlings; PSE meat.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500013

Effects of light and nutrients on different germination phases of the Cosmopolitan moss Bryum argenteum Hedw. (Bryaceae)
Provedor de dados: 52
Autores: Silva,Adaíses Simone Maciel da; Pôrto,Kátia Cavalcanti; Simabukuro,Eliana Akie.
The influence of light and nutrients on the different germination phases of Bryum argenteum spores was studied. The following treatments were used: distilled water and nutrient solution under light (12 h) and continuous darkness. The spores germinated when exposed to light, independent of both medium used. Under darkness, the spores swelled and became chlorophyllous. In the presence of nutrient solution, the germination occurred earlier (after two days) when compared to the distilled water (after more than three days). Nutrients were needed to complete the last germination phase in the majority of spores and to provide the protonemal growth.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bryophyte; Physiology; Germination; Protonema; Light; Nutrients.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400003

Caloric density of Loricariichthys platymetopon (Osteichthyes, Loricariidae) in the Upper Paraná River floodplain, Brazil
Provedor de dados: 52
Autores: Garcia,Daniela Aparecida; Benedito,Evanilde; Takeda,Alice Michiyo.
This study analyzed the muscle energy content (caloric density) of L. platymetopon captured at different sites of the Upper Paraná River floodplain, and potential relationships between the content and various environmental variables (water temperature, oxygen dissolved in water, food availability, relative numeric abundance) and biological variables (body size and reproductive cycle). Quarterly samplings from September 2006 to June 2007 in the lakes and rivers resulted in a sample of 739 specimens, whose muscle caloric density was determined using a calorimeter. Differences between the caloric averages of the males and females and among the different stages of gonadal development were not significant. Caloric density varied over time and space, with two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy; Detritivorous fish; Direct calorimetry; Muscle; Floodplain.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500015

High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
Provedor de dados: 52
Autores: Wanderlei-Silva,Denise; Nobre,Mariana; Gonzaga,Rosa Silva; Viana,Luciana Silva; Ramalho Neto,Eduardo.
The analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; DNA purification; PCR; RFLP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006

Composition and seasonal variation of brachyura and anomura (Crustacea, Decapoda) associated with brown mussel farms at Praia da Cocanha, Brazil
Provedor de dados: 52
Autores: Bernadochi,Ligia Coletti; Silvestri,Fausto; Turra,Alexander.
The composition and seasonal variation of brachyuran and anomuran species associated with mussel farms were evaluated at Praia da Cocanha, São Paulo between May 2007 and February 2008. Nine mussel ropes were sampled at random in each quarter, and 1,208 organisms were identified, comprising five families and 28 species. The most numerous species was the porcellanid Pachycheles laevidactylus (18.5%), followed by the xanthids Acantholobulus schmitti (16.6%), Hexapanopeus paulensis (11.3%), Panopeus americanus (10.2%), and Menippe nodifrons (8.4%). The exotic crab Charybdis hellerii was recorded throughout the study period. The ecological descriptors, except Pielou evenness index, varied significantly over the time. The highest abundance and diversity of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Associated fauna; Mussel culture; Perna perna; Crustaceans; Community ecology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400010

Cultivation of Nannochloropsis sp. in brackish groundwater supplemented with municipal wastewater as a nutrient source
Provedor de dados: 52
Autores: Sousa,Louise Lins de; Hora,Dominique Silveira da; Sales,Emerson Andrade; Perelo,Louisa Wessels.
The aim of this work was to study growth potential of the green microalgae Nannochloropsis sp. using brackish groundwater from a well in the semi-arid northeast region of Brazil as culture medium. The medium was supplemented with (%) 19.4, 22.0, 44.0 and 50.0% of municipal wastewater after UASB treatment as a low-cost nutrient source. The results showed that the culture tested was capable of growing in the brackish groundwater even at salinity levels as low as 2 ppt. Furthermore it was shown that municipal wastewater could be used as a sole nutrient source for Nannochloropsis sp.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalgae; Brackish groundwater; Municipal wastewater; Nannochloropsis sp.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200003

Effect of treatment with alkaline hydrogen peroxide associated with extrusion on color and hydration properties of oat hulls
Provedor de dados: 52
Autores: Galdeano,Melicia Cintia; Grossmann,Maria Victória Eiras.
An incomplete factorial design with three independent variables at three levels of variation was used to evaluate the effect of the treatment with alkaline hydrogen peroxide concomitant with extrusion on some properties of oat hulls. The independent variables were hydrogen peroxide level, feed moisture and extrusion temperature. The dependent variables were water retention capacity (WRC), swollen volume (SV) and color. The analysis of variance showed that temperature was the most important variable for the hydration properties. The highest values of WRC and SV were obtained when oat hulls were processed in the condition of 7% hydrogen peroxide, 32% feed moisture and 90ºC extrusion temperature. Modified hulls had a WRC 70% higher and SV 55% higher than the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dietary fiber; Oat hulls; Chemical modification; Functional properties.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100010

Relationships of photosynthetic photon flux density, air temperature and humidity with tomato leaf diffusive conductance and temperature
Provedor de dados: 52
Autores: Righi,Evandro Zanini; Buriol,Galileo Adeli; Angelocci,Luiz Roberto; Heldwein,Arno Bernardo; Tazzo,Ivonete Fátima.
The objective was to study the leaf temperature (LT) and leaf diffusive vapor conductance (gs) responses to temperature, humidity and incident flux density of photosynthetically active photons (PPFD) of tomato plants grown without water restriction in a plastic greenhouse in Santa Maria, RS, Brazil. The plants were grown in substrate and irrigated daily. The gs was measured using a steady-state null-balance porometer on the abaxial face of the leaves during the daytime. Both leaf surfaces were measured in one day. The PPFD and LT were measured using the porometer. Leaf temperature was determined using an infrared thermometer, and air temperature and humidity were measured using a thermohygrograph. The leaves on the upper layer of the plants had higher gs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Controlled environment; Stomatal behavior; Air vapor pressure deficit; Leaf-to-air vapor pressure deficit.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300005

A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
Provedor de dados: 52
Autores: Acevedo,Francisca; Pizzul,Leticia; Castillo,María del Pilar; Rubilar,Olga; Lienqueo,María Elena; Tortella,Gonzalo; Diez,María Cristina.
In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: White-rot fungi; Ligninolytic enzymes; Purification; Lignocellulosic material.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013

Comparative Analysis of C-repeat Binding Factors (CBFs) in Tomato and Arabidopsis
Provedor de dados: 52
Autores: Heidari,Parviz.
Abstract Cold stress is one of the limiting factors of plant production that plants use different mechanisms for cold tolerance. CBF genes play critical role to regulate the cold responsive genes. To better understand of CBF gene functions, the tomato-CBFs and Arabidopsis-CBFs were evaluated using bioinformatics tools, and finally the expression patterns of SlCBF1 gene were analyzed under 10 and 4˚C in two contrasting tomato species (Solanum lycopersicum and S. habrochaites). The different cis regulatory elements were observed in promoter region of SlCBF1 and AtCBF1 genes, and ICE1, COR and HOS1 proteins exhibited high interaction with CBFs. The results of Real time PCR of SlCBF1 exhibited that under 10 and 4 ˚C, SlCBF1 was down regulated in cold sensitive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cold stress; CBF genes; Real time PCR; Tomato; Bioinformatics analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100208

Influence of effluents from a Wastewater Treatment Plant on nutrient distribution in a coastal creek from southern Brazil
Provedor de dados: 52
Autores: Santos,Isaac Rodrigues; Costa,Ronaldo Cataldo; Freitas,Ubiratan; Fillmann,Gilberto.
The hypothesis that effluents treated through activated sludge process cause changes in nutrient biogeochemistry of receiving water bodies was investigated in Vieira creek, southern Brazil. Dissolved oxygen, suspended matter, and pH did not vary among the sampling stations. Nutrient, biochemical oxygen demand, and conductivity values were significantly higher downstream from the Wastewater Treatment Plant (WWTP) effluents. Further downstream, nitrate concentrations were higher due to ammonium nitrification, organic matter remineralization and/or the occurrence of unidentified sources. Per capita nutrient emission factors were estimated to be 0.16 kg P.yr-1 and 4.14 kg N.yr-1. Under pristine conditions, low N:P ratios were observed, which were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activated sludge; Nutrients; Contamination; Coastal zone; Biogeochemistry; Eutrophication.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100019

Effects of different temperatures on the performance of seeds germination of Cecropia pachystachya Trec. (Cecropiaceae)
Provedor de dados: 52
Autores: Pilati,Rosemari; Andrian,Izabel de Fátima; Carneiro,José W. P.
Seed germination of Cecropia pachystachya Trec. was investigated at 25, 30, and 35ºC using fluorescent day lights. Ten replicates each of 200 seeds were used in each thermal treatment. Germitest® paper, CEL-065®, and 11x11x5 cm Gerbox® were also used. The frequencies of radicle protrusions were counted at consecutive two hour intervals. The cumulative frequencies were combined with the distribution of Weibull using the following model: Y = M (1 - exp (-(t/b)c)). The best performance was achieved with the temperature of 30ºC ( M, 95.29%). Time to achieve 60.23% of seed germination (0.6321 x 95.29%) was 99.35 h with a spread of 7.19. All model/data set combinations had close to linear.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cecropia pachystachya; Seed germination; Weibull function.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200010

Population dynamics of mixed cultures of yeast and lactic acid bacteria in cider conditions
Provedor de dados: 52
Autores: Dierings,Leila Roseli; Braga,Cíntia Maia; Silva,Karolline Marques da; Wosiacki,Gilvan; Nogueira,Alessandro.
The objective of this work was to study the malolactic bioconversion in low acidity cider, according Brazilian conditions. The apple must was inoculated with Saccharomyces cerevisiae or S. cerevisiae with Oenococcus oeni. The control contained the indigenous microorganisms. Fermentation assays were carried out with clarified apple must from the Gala variety. At the beginning of fermentation, there was a fast growth of the non-Saccharomyces yeast population. Competitive inhibition occurred in all the assays, either with inoculated or indigenous populations of the yeast. The lactic acid bacteria count was ca. 1.41·10²CFU/mL at the beginning and 10(6)CFU/mL after yeast cells autolysis. The lactic bacteria O. oeni reached the highest population (10(7)CFU/mL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apiculate yeast; Cider; Lactic acid bacteria; Alcoholic fermentation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500016

The physiological and biochemical mechanism of nitrate-nitrogen removal by water hyacinth from agriculture eutrophic wastewater
Provedor de dados: 52
Autores: Wenwei,WU; Ang,LIU; Konghuan,WU; Lei,ZHAO; Xiaohua,BAI; Kun-zhi,LI; ASHRAF,Muhammad Aqeel; Limei,CHEN.
ABSTRACT Large amount of agriculturl wastewater containing high level nitrate-nitrogen (NO3 --N) is produced from modern intensive agricultural production management due to the excessive use of chemical fertilizers and livestock scale farming. The hydroponic experiment of water hyacinth was conducted for analyzing the content of NO3 --N, soluble sugar content, N-transported the amino acid content and growth change in water hyacinth to explore its purification ability to remove NO3 --N from agriculture eutrophic wastewater and physiological and biochemical mechanism of this plant to remove NO3 --N. The results showed that the water hyacinth could effectively utilize the NO3 --N from agriculture eutrophic wastewater. Compared with the control, the contents...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water hyacinth; Nitrate-nitrogen removal; Nitrate conversion; N-transported amino acids.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200200

Efficient Regeneration of Eucalyptus urophylla x Eucalyptus grandis from Stem Segment
Provedor de dados: 52
Autores: Ouyang,Lejun; Huang,Zhenchi; Zhao,Longyan; Sha,Yuee; Zeng,Fuhua; Lu,Xiangyang.
The aim of the present study was to establish an efficient regeneration system for the hybrid E. urophylla x E. grandis by means of organogenesis. Stem segments from seedlings were used as explants and cultured in a modified Murashige and Skoog medium (MS), supplemented with 13.2 µM N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285 µM indole-3-acetic acid (IAA). PBU was a useful growth regulator. After cultivating for 5 d, 96% explants formed callus. After 30 d, the calli obtained were transferred to MS medium containing different combinations of 6-benzyladenine (BA) and naphthalene acetic acid (NAA). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning. In addition, a large percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro regeneration; Bud induction; Proliferation; Substituted urea.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300001

Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
Provedor de dados: 52
Autores: Urek,Raziye Ozturk; Pazarlioglu,Nurdan Kasikara.
Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phanerochaete chrysosporium; Manganese dependent peroxidase; Tween 80; Mn2+; Phenylmethylsulphonylfloride.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700001

Monitoring the effect of pyrene on the germination and radial growth of the wild and mutant strains of Rhizopus arrhizus UCP402
Provedor de dados: 52
Autores: Shiosaki,Ricardo Kenji; Albuquerque,Clarissa Daisy da Costa; Okada,Kaoru; Fukushima,Kazutaka; Campos-Takaki,Galba Maria.
The physiological mutant of Rhizopus arrhizus was obtained in the pyrene resistance gradient test. Comparative studies were carried out about the behavior of the germination process and the radial growth of the mutant and wild strains of R. arrhizus UCP 402. Sabouraud Sucrose and Yeast Malt Broth cultures containing pyrene (10 mg/L) induced the germination process of the sporangiospores of the wild and mutant strains of R. arrhizus. The radial growth of the strains was inversely proportional to the pyrene concentration in the culture medium. The results showed an adaptation of R. arrhizus UCP 402x (mutant) in the pyrene (50mg/L) and suggested a higher ability of application in the removal of pyrene from the contaminated areas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrene; Rhizopus arrhizus; Mutant; Radial growth; Germination.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300024

The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation
Provedor de dados: 52
Autores: Hu,Wei; Chen,Jihong; Wu,Qinghua; Li,Wenjian; Liu,Jing; Lu,Dong; Wang,Shuyang.
ABSTRACT Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provided to investigate carbon or nitrogen source utilization between mutant SRZ50 and wild type, and different fermentation periods were also chose to study fermentation characteristic between mutant SRZ50 and wild type. The results showed that mutant SRZ50 exhibited the enhanced L-(+)-lactic acid production from wild type. When glucose or fructose was the sole carbon source, the L(+)-lactic acid production...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L. thermophilus; Mutant; Heavy ion irradiation; L-(+)-lactic acid production; Carbon source; Fermentation characteristic.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100504

Validation of an ovarian biopsy method for monitoring oocyte development in the fat snook, Centropomus parallelus Poey, 1860 in captivity
Provedor de dados: 52
Autores: Ferraz,Eduardo Medeiros; Alvarez-Lajonchère,Luis; Cerqueira,Vinicius Ronzani; Candido,Sidinei.
The validation of an ovarian biopsy method for in vivo assessment of oocyte maturation in Centropomus parallelus was studied. Diameters of intra-ovarian oocytes siphoned with cannula were analyzed fresh and preserved with 1% formalin in 0.7% NaCl solution. Oocytes in different stages were present along the ovaries, up to the tertiary yolk globule stage, which had a unimodal diameter frequency distribution. The oocyte diameter means were not significantly different at four sites along the ovaries (P &gt; 0.05). Samples obtained with cannula were representative of the ovary central portion, in vivo and in vitro samples of the seven females examined were not significantly different (P &gt; 0.05). An estimate of the coefficient of variation corrected for bias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovarian Biopsy; Fat Snook; Centropomus parallelus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400018

Evaluation of protein quality from genetically modified and organic soybean in two consecutives generations of wistar rats
Provedor de dados: 52
Autores: Daleprane,Julio Beltrame; Pacheco,Juliana Tomaz; Boaventura,Gilson Teles.
The aim of this study was to evaluate the cumulative effects of using genetically modified and organic soybean in two generations of rats. Two consecutive generations of 64 Wistar rats denominated F0 and F1 were used. The animals from each generation were divided into three groups (n=8) and fed chow made of organic soybean, genetically modified soybean and casein. The PER, NPR and CAE were determined. ANOVA was applied to the results. In both the generations, a statistically significant (p&lt;0.05) difference was found between the experimental groups and the control group. The organic soybean supplement with L-cystine provided a better use of this protein in comparison to the genetically modified soybean protein, which demonstrated that the soybean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybeans; Wistar rats; Protein quality; Generations.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400007

Chemical quality of bottled mineral waters from markets of Curitiba-PR-Brazil
Provedor de dados: 52
Autores: Santos,Éder José dos; Oliveira Junior,Dasio Roberto de; Hermann,Amanda Beatriz; Sturgeon,Ralph Edward.
ABSTRACT Twenty-seven bottled mineral waters from local markets in Curitiba, State of Paraná, Brazil were analyzed for a number of constituents, including: pH, conductivity, total dissolved solids, hardness, HCO3  - and CO 3  2-, as well as Ca2+, Mg2+, Na+, K+, NH4 + , F-, Cl-, NO3 -, NO2 - and SO4 2- by ion chromatography (IC); Al, Ba, Cd, Cr, Cu, Fe, Li, Mn, Ni, Sr, V and Zn by inductively coupled plasma optical emission spectrometry (ICP OES); Hg by cold vapor generation-ICP OES and As, Pb, Sb and Se by ICP-mass spectrometry (ICP-MS). The obtained limits of quantitation (LOQ) were adequate for the determination of all analytes using the various analytical techniques. Results for the analysis of AccuStandard certified reference materials QCS-01-5,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bottled mineral waters; Elemental composition; Chemical quality; Principal component analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100513

Eugenia Klotzschiana O. Berg Fruits as New Sources of Nutrients: Determination of their Bioactive Compounds, Antioxidant Activity and Chemical Composition
Provedor de dados: 52
Autores: Carneiro,Nárgella Silva; Alves,Cássia Cristina Fernandes; Cagnin,Caroline; Belisario,Celso Martins; Silva,Marco Antônio Pereira da; Miranda,Mayker Lazaro Dantas; Oliveira Filho,Josemar Gonçalves de; Alves,José Milton; Pereira,Paulo Sérgio; Silva,Fabiano Guimarães; Egea,Mariana Buranelo.
Abstract: The Cerrado is one of the world's biodiversity hotspots and Brazil’s second largest biome. Many native species of the Brazilian Cerrado provide fruits that have unique sensory characteristics and high nutritional value. This study aimed at characterizing the pulp of Eugenia klotzschiana O. Berg, concerning its proximal composition, bioactive compound content and antioxidant activity. The pulp under study had high moisture (89.47 g kg-1) and caloric (96.07 kcal kg-1) values whereas its contents of protein (0.59 g kg-1) and lipids (2.35 g kg-1) were low. The cerrado pear pulp also had high iron content (16.5 mg kg -1) and dietary fiber (6.45 g kg-1), besides 0.034-0,055 mg kg-1 carotenoids, 8.66 mg kg-1 ascorbic acid and 0.66 mg kg-1 total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado pear; Nutrients; Flavonoids; Antioxidant capacity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100402

Multivariate analysis of the spectroscopic profile of the sugar fraction of apple pomace
Provedor de dados: 52
Autores: Gabriel,Luciana Sutil; Prestes,Rosilene Aparecida; Pinheiro,Luís Antonio; Barison,Andersson; Wosiacki,Gilvan.
Pomace, the main by-product of apple juice processing, contains all the fruit's compounds such as minerals, sugars, fibers, enzymes and secondary metabolites after bioprocessing. Dried pomace from four apple varieties (Gala, Fuji, Catarina and Joaquina) was used to extract fructose, glucose, and sucrose, the main soluble sugars contained in apple pomace, to verify the possible use of the sugar fractions. The concentrated sugars were characterized by NMR and FTIR. The partial least squares method (PLS) applied to the NMR and FTIR spectra revealed large amounts of fructose, glucose, and sucrose, without the presence of others compounds. Principal component analysis (PCA) discriminated the studied apple varieties, with a 99% level of significance, as function...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple pomace; NMR; FTIR; Multivariate analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300012

Effect of the use of curing salts and of a starter culture on the sensory and microbiological characteristics of homemade salamis
Provedor de dados: 52
Autores: Spricigo,Cinthia Bittencourt; Pianovsky,Patrícia Bonat.
Homemade salamis may have their food safety guaranteed by means of the addition of curing salt and starter cultures, without loosing their traditional manufacturing recipes brought to Brazil by the Italian immigration in the early 20th century. In this work, the influence of curing salt and of a starter culture, composed of Lactobacillus and Staphylococcus, over the sensory and microbiological characteristics of Italian type salamis, containing 3% lactose and 0.5% saccharose, was evaluated. The starter culture and the curing salt inhibited the development of Staphylococcus aureus and of coliforms, and the salamis added with curing salt presented better color attributes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curing salt; Food safety; Salami; Starter culture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400021

Preliminar evaluation of performance in topcrosses between vegetable type and grain type soybean
Provedor de dados: 52
Autores: Yokomizo,Gilberto K.; Vello,Natal A.; Nekastchalow,Marcos C.
Studies were conducted to evaluate the performance of topcrosses between food type soybean (with tendency to low physiological quality of the seeds) with two adapted grain type, seeking to associate adequate human food characteristics with adaptability. The conclusions were: a) The best topcrosses for vegetable type soybean were obtained predominantly with ´FT-2` and for seed yield with ´Doko`; b) The evaluated topcrosses presented high sensibility to winter conditions in comparison to summer, consequently the selection process in the winter it must be avoided; c) The selection for food type soybean it must be established in adequate values between individual plant yield and one hundred seed weight.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Seed size; Vegetal breeding; Vegetal germplasm.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300004

Studies on production of single cell protein by Aspergillus niger in solid state fermentation of rice bran
Provedor de dados: 52
Autores: Anupama,; Ravindra,Pogaku.
An attempt was made to apply the solid state fermentation (SSF) for the production of single cell protein (SCP) using oil free rice bran waste as substrate. A local isolate of Aspergillus niger, was used as protein source for the studies. Total proteins were extracted to estimate the mycelial biomass from the moldy bran. Carbonate-bicarbonate extraction buffer and a pH 10 was found to be most efficient among the buffers used for the extraction of the proteins from the organism. The effect of supplementation by various sources of nitrogen and mineral solution on the final biomass yield was compared. The influence of C/N ratio on the protein yield was also studied. Sodium nitrate at C/N ratio of 1.387 was found to be an effective nitrogen-supplementing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single cell protein; Solid State Fermentation; Aspergillus niger; Rice bran; C/N ratio.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100011

Phytotoxicity of Perlatolic Acid and Derivatives
Provedor de dados: 52
Autores: Peres,Marize Terezinha; Cândido,Ana Carina S.; Faccenda,Odival; Gianini,Aline Siqueira; Honda,Neli Kika.
ABSTRACT Perlatolic acid (1), methyl perlatolate (2), and the products of perlatolic acid alcoholysis-namely, methyl, ethyl, n-propyl, iso-propyl, n-butyl, sec-butyl, tert-butyl, n-pentyl, and n-hexyl 2-hydroxy-4-methoxy-6-n-pentylbenzoate (3-11)-were evaluated for their herbicidal potential on Lactuca sativa L. (lettuce) and Allium cepa L. (onion) seeds. The compounds exhibited low phytotoxicity on L. sativa germination. Perlatolic acid (1) proved the most active compound (20%). Activity was lowest for n-hexyl 2-hydroxy-4-methoxy-6-n-pentylbenzoate (11) (3.5%). The esters iso-propyl and sec-butyl 2-hydroxy-4-methoxy-6-n-pentylbenzoate (6 and 8, respectively) inhibited root (35% e 43%, respectively) and hypocotyl growth (59% e 56%, respectively). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Perlatolic acid; 2-hydroxy-4-methoxy-6-n-pentylbenzoates; Lactuca sativa; Allium cepa; Allelopathy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100205

Establishment of the carbon label mechanism of coal chemical products based oncarbon footprint
Provedor de dados: 52
Autores: Bishan,Wu; Zhenling,Liu; Ashraf,Muhammad Aqeel.
ABSTRACT After redefining the carbon footprint and carbon label, the paper analyzesthe significance of the carbon labels under the background of the low carbon economy development, and establishes the concept of model of the carbon labels mechanism to chemical products. At the same time, the paper quantitatively studies carbon label data sourceof three kinds of coal chemical industry power products, which are fromhaving not CCS technologies of supercritical boiler of coal, using CCS technologies of supercritical boiler of coal and adopting CCS and IGCC technologies to power generation in CCI. Based on the three kinds of differences, the paper puts forward of establishing the carbon labels mechanism of chemical products under the low carbon consumption.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbon label; Carbon footprint; Coal chemical industry; Carbon capture and storage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200702

In silico analysis of compounds from Stemona tuberosa as an inhibitor for N1 neuraminidase of H5N1 avian virus
Provedor de dados: 52
Autores: Manohar,Abhilash.
The worldwide spread of H5N1 avian influenza has raised concerns that this virus might acquire the ability to pass readily among humans and cause a pandemic.Two anti-influenza drugs currently being used to treat the infected patients are oseltamivir (Tamiflu) and zanamivir (Relenza), both of which target the neuraminidase enzyme of the virus. Reports of the emergence of drug resistance has made the development of new anti-influenza molecules a priority. Various compounds present in Stemona tuberosa a plant belonging to Stemonaceae family was tested for activity against H5N1 neuraminidase. Eight one molecules including stilbenoids, bibenzyls and various others were selected as probable compounds for lead molecules. These lead molecules were tested for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H5N1 Avian virus; Neuraminidase inhibiotors; Stemona tuberosa; Docking analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100003

Hepatoprotective effects of Iranian Hypericum scabrum essential oils against oxidative stress induced by acetaminophen in rats
Provedor de dados: 52
Autores: Dadkhah,Abolfazl; Fatemi,Faezeh; Farsani,Mohsen Eslami; Roshanaei,Kambiz; Alipour,Mahdi; Aligolzadeh,Hadi.
This studied examined the protective role of Hypericum scabrum oils (100 and 200 mg/kg b.w, i.p) on acetaminophen-induced liver damages in the rat. The hepatic oxidative/antioxidant parameters such as lipid peroxidation (LP), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and ferric reducing ability of plasma (FRAP) were measured 2, 4, 8, 16 and 24h after the treatments confirmed by histopathological consideration. The results indicated that increased levels of hepatic LP and FRAP and SOD activity were reversed in the rats treated with oils. In addition, the depleted GSH were compensated with the oil treatments. The protective effect of the oils was further confirmed by the histophatological examination carried out on liver biopsies. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypericum scabrum; Essential oils; Acetaminophen; Rat; Oxidative stress; Liver.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300005

Structural analysis of Castanea sativa Mill. leaves from different regions in the tree top
Provedor de dados: 52
Autores: Pinto,Teresa Maria; Anjos,Maria do Rosário; Martins,Nuno Miguel; Gomes-Laranjo,José; Ferreira-Cardoso,Jorge; Peixoto,Francisco.
The aim of this work was to perform the histological characterization of the C. sativa leaves of three Portuguese cultivars to establish comparison among the leaves of the different quadrants in accord and with the cardinal points of the tree top and among different cultivars of this species, using light microscopy (OM) and scanning electron microscopy (SEM). Measurements were also carried out for the leaf tissue thickness, stomatal density, leaf area in the four tree top quadrants. The leaves turned to the North had lesser thickness of mesophyll mainly due to lower amount of palisade parenchyma. The stomatal density was significantly lower in these leaves, unlike the leaf area that has the highest expression.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. sativa; Leaf anatomy; Palisade parenchyma; Spongy parenchyma; Stomatal density; Scanning electron microscopy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100016

Reorganization and cleanness of peridomiciliar area to control sand flies (Diptera, Psychodidae, Phlebotominae) in South Brazil
Provedor de dados: 52
Autores: Teodoro,Ueslei; Thomaz-Soccol,Vanete; Kühl,João B.; Santos,Demilson Rodrigues dos; Santos,Elcio Silvestre dos; Santos,Ademar Rodrigues dos; Abbas,Milia; Dias,Alessandra de Cassia.
The objective of the present work was to evaluate the impact of the reorganization and cleaning of the peridomicily seeking the control of Phlebotominae vectors of Leishmania. Collections of insects were made in domestic animal shelters and in peridomiciliar area located in Jussara farm, Municipality of Jussara, State of Parana, Brazil where cutaneous leishmaniasis has been endemic. The collections were accomplished five nights for month of the 21:00pm for fie hours, in 1996 and 1997. The results were compared the those obtained in the same places in 1992 and 1994. After the collections of 1992 peridomiciliar area was cleaned and organic matter was removed and domestic animals were reallocated. In 1992, 1994 and 1996/97, 10, 7 and 12 Phlebotominae species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phlebotominae control; Leishmaniasis control; Lutzomyia whitmani; Cutaneous leishmaniasis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200007

Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)
Provedor de dados: 52
Autores: Silva,Paula Viana Correa da; Lui,Jeffrey Frederico; Band,Guilherme de Oliveira; Regitano,Luciana Correia de Almeida; Grossi,Selma de Fátima; Sollero,Bruna Pena; Nunes,Cleujosí da Silva.
The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (F IS ) within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular genetics; Wild boars; Microsatellites; Fragments; Amplification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200011

Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents
Provedor de dados: 52
Autores: Masi,Chandran; Chandramohan,C.; Ahmed,M.Fazil.
ABSTRACT Protease is an enzyme which has a wide range of applications in various fields. Extracellular protease was produced from Pseudomonas aeruginosa and Enterococcus hirae which were isolated from the effluents of diary industries. Protease immobilized with super paramagnetic nanoparticles was characterized by DLS, XRD and TEM methods in relation to their size and structure. The protease enzyme was bound to magnetic nanoparticles via surface transformation technique including Silica coated magnetic Nano composite, amine and cysteine functioned Nano composite formation. Successful binding of protease onto the particles was confirmed by TEM imaging. The maximal enzyme activity of immobilized protease was determined using universal protease assay and was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Super paramagnetic Nanoparticle; Nano composite; Immobilization; Activity and Stability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100462

Remediation of polluted soils contaminated with Linear Alkyl Benzenes using Fenton's reagent
Provedor de dados: 52
Autores: Silva,Douglas do Nascimento; Zagatto,Paulo José Piacentini; Guardani,Roberto; Nascimento,Cláudio Augusto Oller.
Linear Alkyl Benzenes (LABs) are used as insulating oil for electric cables. When it happens a spill, LABs they are basically sorbed in the soil, because, these compounds have high hidrophobicity and low vapor pressure. The conventional methods of treatment of soils are not efficient. The Fenton's reaction (reaction between a solution of iron II and hydrogen peroxide) it generates hydroxyl radicals, not selective, and capable of oxidize a great variety of organic compounds. A study was conducted to evaluate the viability of use of the Fenton's reagents to promote the remediation of polluted soils with Linear Alkyl Benzenes. A column was especially projected for these experiments, packed with a sandy and other soil loamy. The pH of the soil was not altered....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soil Remediation; Fenton's Reagent; Linear Alkyl Benzene.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400032

Analysis of the Insertion of Particular Photovoltaic Generation in the Low Voltage Installations, Under the Approach to Performance, Safety, Maintenance and Operation of the PVS
Provedor de dados: 52
Autores: Moraes,Fillipe Alexandre; Takeda,Othavio Toniasso.
ABSTRACT The growing use of Photovoltaic Solar Energy in Brazil, both in power plants and in distributed generation systems, brings with it a series of practical and technical questions that are not limited to the design and project of the systems. Environmental factors and resources related to the use, operation, maintenance, decommissioning and final disposal of the equipment and components that constitute them, should be taken into consideration, so that these systems maintain an adequate performance during their lifespan granting the safety for users, properties and avoid negative environmental impacts. Within the concept of Risk Analysis within the Life Cycle of these systems, the present study addresses a perspective of the growth of the use of Solar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic Installations; Maintenance; Degradation; Reliability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200234

Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36
Provedor de dados: 52
Autores: Danial,Enas Nabil; Ayaz,Najla Osman Aly; Alnahdi,Hanan Saeed Omer.
The aim of this work was to optimize the growth conditions and continuous production of the enzyme using free and immobilized cells of inulinase by Penicillium funiculosum. The highest yield of enzyme (163.5U/mL) was obtained when the culture was incubated at 27oC and 200 rpm for 96h in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen source, respectively. When the cells of the P. funiculosum were immobilized on different carriers, especially linen fibers, their production ability was successfully maintained for seven successive batches. When the fermentation was carried out using inulin juice prepared from Jerusalem artichoke tubers (in place of pure inulin), inulinase production could be sustained till the second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium funiculosum; Biochemical inulinase; Inulin; Repeated batch cultivation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400636

Planktonic and non-planktonic rotifers in two environments of the Upper Paraná River floodplain, state of Mato Grosso do Sul, Brazil
Provedor de dados: 52
Autores: Bonecker,Cláudia C.; Lansac-Tôha,Fábio A.; Rossa,Dayane C.
Investigations were carried out for the composition and occurrence of rotifer taxa of different forms and sizes during two distinct hydrological periods, in two environments (river and lake). Two sampling stations were established in open water of each environments. Surface samples were taken daily for 14 days. The faunistic similarity between the environments was established using Jaccard's index. One hundred and fourty three taxa were identified. The largest number of taxa was recorded in the river. Faunistic similarity between these environments was greater during high water. The planktonic taxa predominated and the difference for the non-planktonic ones was not marked. Non-planktonic taxa predominated in the river, while both types were numerous in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotifers; Size classes; Habitat; Floodplain; Brazil.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400009

Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802)
Provedor de dados: 52
Autores: Carlos,Jaqueline; Matta,Sérgio Luis Pinto da.
The aim of this work was to study the testicular morphometry of captivity-bred adult bullfrogs. Fifteen young adult male were studied, in the rainy season and a lengthy photoperiod. The GSI was established at 0.15%. The nuclear diameter of germinative and Leydig cells, the nucleolus diameter of Sertoli cells and the area of cysts and tubules were determined and the mean number of ISPC, IISPC and SPT per cyst and the mean number of cysts per tubule was estimated. The nucleoplasmatic proportion of the nucleus of the Leydig cell was 76.22%, indicating less cytoplasmic activity. Eight generations of spermatogonia were found. The spermatogenesis efficiency in meiosis and in mitosis was 63 and 49%, respectively. The spermatogenesis of bullfrog fited in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogenesis; Sertoli; Leydig; GSI.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600018

Allochthonous matter input in two different stretches of a headstream (Itatinga, São Paulo, Brazil)
Provedor de dados: 52
Autores: Afonso,Ana Adelina de O.; Henry,Raoul; Rodella,Rita C.S.Maimoni.
In two streches of the Itaúna stream, one in a gallery forest and the other in an herbaceous vegetation zone, litter matter input (sorted by leaves, ligneous and reproductive components) were measured during a year. In the gallery forest stretch, the direct input and lateral transport attained 713 g.m-2.year-1 and 421 g.m-1. year-1, respectively. In contrast, the herbaceous vegetation stretch showed a direct litter input and lateral transport of only 86 g.m-2.year-1 and 124 g.m-1. year-1, respectively. The empoverishment of riparian vegetation had a significant effect on the decrease of allochthonous input to stream mainly by direct input. Peaks of direct input into Itaúna stream occurred during late dry season and in the early wet season. They were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allochthonous input; Stream; Riparian vegetation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300014

Populational fluctuation of vectors of Xylella fastidiosa, wells in sweet orange [Citrus sinensis (L.) Osbeck] varieties of northwest Paraná State, Brazil
Provedor de dados: 52
Autores: Molina,Rúbia de Oliveira; Gonçalves,Aline Maria Orbolato; Zanutto,Carlos Alexandre; Nunes,William Mário de Carvalho.
The aim of the present study was to assess the population flutuation of the sharpshooters species subfamily Cicadellinae belonging to the tribes Cicadellini and Proconiini, in sweet orange [Citrus sinensis( L). Osbeck] commercial orchards of the northwest region of Paraná State , Brazil. Samplings were carried out the employing every time 24 yellow sticky cards. Identification of the species showed that the most representative were Dilobopterus costalimai of the Cicadellini tribe and Acrogonia citrina of the Proconiini tribe.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sharpshooter; Cicadellidae; Citrus variegated chlorosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300007

Natural regeneration in a quaternary coastal plain in southern Brazilian Atlantic Rain forest
Provedor de dados: 52
Autores: Salimon,Cleber Ibraim; Negrelle,Raquel R. B.
Composition, structure and dynamics of an eight year old secondary forest was studied at Reserva Volta Velha (26°04'S; 48°38'W), southern Brazil. A 0.72ha plot was divided into 36 subplots of 20X10m, where all trees/shrubs greater than 1m tall were identified, measured (height/diameter) and evaluated (successional status). The results were: (1) 95 species collected within 68 genera and 44 families; the most species rich families were Myrtaceae and Asteraceae with 8 species each; (2) the most important species (considering biomass and density) were Psidium cattleianum, Eupatorium casarettoi, Ocotea pulchella and Ternstroemia brasiliensis; (3) the most similar area was a fallow abandoned 35 years ago; (4) the higher species diversity were found in border...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic Rain Forest; Floristics; Structure; Regeneration; Dynamics.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200008

Gene Expression Analyses of HER-2/neu and ESR1 in Patients with Breast Cancer
Provedor de dados: 52
Autores: Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza.
ABSTRACT Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features. Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software. Results: HER2 and ESR1 overexpression was identified in 19...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100317

Lipid oxidation and fatty acid profile related to broiler breast meat color abnormalities
Provedor de dados: 52
Autores: Soares,Adriana Lourenço; Marchi,Denis Fabrício; Matsushita,Makoto; Guarnieri,Paulo Donizeti; Droval,Adriana Aparecida; Ida,Elza Iouko; Shimokomaki,Massami.
The aim of this work was to study the influence of lipid oxidation on broiler breast meat (Pectoralis major m) color abnormalities. There were 27.0 % more lipid oxidation in PSE in relation to normal meat and 41.0 % more in relation to DFD-like meat (p&lt;0.05). The fatty acid profile was also significantly different since the arachidonic acid (AA) fraction increased 38.6 and 70.5 % in PSE meat comparing to normal and DFD-like meats, respectively. The ratio PUFA/SFA changed in these three types of meat: 0.736, 0.713 and 0.694 for PSE, normal and DFD-like meat respectively, reflecting the highest production of polyunsaturated fatty acids in the PSE meat samples. Results indicated that phospholipase A2 enzyme activities played a relevant role towards the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PSE meat; DFD meat; Arachidonic acid.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600023

Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation
Provedor de dados: 52
Autores: Ribas,Alessandra Ferreira; Kobayashi,Adilson Kenji; Pereira,Luiz Filipe Protasio; Vieira,Luiz Gonzaga Esteves.
Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl)-adenine (2-iP). Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic transformation; Coffee; Herbicide tolerance; Ammonium glufosinate.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100002

First Report of Albinism in the Threatened Gillbacker Sea Catfish Sciades parkeri (Siluriformes, Ariidae)
Provedor de dados: 52
Autores: Andrade,Marcelo C.; Schmid,Kurt; Souza Junior,Oswaldo; Giarrizzo,Tommaso.
ABSTRACT A sexually mature albino specimen of the Gillbacker Sea Catfish species Sciades parkeri (Siluriformes, Ariidae), a vulnerable species, was captured in northern Amazon estuary, State of Pará, Brazil. Herein we present the first record of albinism phenomenon for this species, including the morphometric description of the albino, and a non-abnormal coloring specimen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon; Delta; Estuary; Marajó; Vulnerable.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100416

Multiple bovine papillomavirus infections associated with cutaneous papillomatosis in brazilian cattle herds
Provedor de dados: 52
Autores: Claus,Marlise Pompeo; Lunardi,Michele; Alfieri,Amauri Alcindo; Otonel,Rodrigo Alejandro Arellano; Sartori,Daniele; Fungaro,Maria Helena Pelegrinelli; Alfieri,Alice Fernandes.
Cutaneous papillomatosis is a pathological condition commonly found in cattle and is characterized by the presence of benign proliferative tumors caused by bovine papillomavirus (BPV) infection. While multiple infections with human papillomavirus (HPV) are common in healthy and immunodeficient humans, studies with the aim of identifying mixed infections are still sporadic in veterinary medicine. The aim of this study is to describe the occurrence of multiple BPV infections in cattle affected by cutaneous papillomatosis. Fifteen skin warts were collected from at least two diverse anatomical regions of six bovines with papillomatosis belonging to three cattle herds from the Paraná state in Brazil. The BPV types present in the skin wart samples were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Skin warts; BPV; Mixed infections.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700013

Spore germination of Gleichenella pectinata (Willd.) Ching (Polypodiopsida-Gleicheniaceae) at different temperatures, levels of light and pH
Provedor de dados: 52
Autores: Santos,Eunice Pereira Gonçalves dos; Lehmann,Débora Rosana Marques; Santos,Marisa; Randi,Áurea Maria.
This study investigated the effects of different temperatures (25 and 30 ± 2 ºC), light levels (62, 42, 22 and 5% of natural light), and pH (4.0, 4.5, 5.0, 5.5, 6.0 and 6.7) on the spore germination of Gleichenella pectinata. To accomplish this, the spores were surface sterilized and sown in bottles containing mineral medium. The spores of G. pectinata are monolets and the perispore surface can show large and irregular deposits. During germination, filamentous gametophytes with and without developing rhizoids were observed. Only gametophytes which presented developed rhizoids were able to reach the heart-shaped developmental stage. Based on the collection, the rate of and gametophyte development were generally very low. The relative germination rates were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gleichenella pectinata; Germination; Light; PH; Temperatures.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600007

Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan
Provedor de dados: 52
Autores: Zahoor,Sana; Javed,Muhammad Mohsin; Babarl,Masroor Ellahi.
ABSTRACT A thermophilic bacterium (TP-2) was isolated from the Tatta Pani hot spring in Azad Kashmir and was characterized using phenotypic and genotypic characters. The strain developed cream colored, round, smooth, flat and slimy colonies while the cells were Gram positive rods that ranged in size from about 2.1-3.6 μm to 0.2-0.3 μm in width. Sequence analysis of its 16S rRNA gene showed that isolate TP-2 had 89% homology with Geobacillus debilis. It grew within pH range of 5.5 to 8.5 with optimum growth at pH 7.0. The isolate showed optimum growth at 65ºC and gave positive results for gelatin hydrolysis (GEL), ortho nitrophenyl-β-D-galactopyranosidase (ONPG), and nitrate production and produced acid from sucrose, glucose and maltose. It utilized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tatta Pani; Characterization; Enzyme production; Geobacillus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100338

Solid-state fermentation for the enrichment and extraction of proteins and antioxidant compounds in rice bran by Rhizopus oryzae
Provedor de dados: 52
Autores: Kupski,Larine; Cipolatti,Eliane; Rocha,Meritaine da; Oliveira,Melissa dos Santos; Souza-Soares,Leonor de Almeida; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the solid-state fermentation with Rhizopus oryzae CCT 7560 of rice bran for the enrichment of proteins and the antioxidant compounds in the fermented biomass. Fermentation was performed in tray bioreactors at 30ºC for 120 h. Protein extraction was done at alkaline pH, followed by precipitation with acetone. Phenolic compounds were extracted with cold methanol. The maximum protein was recovered from after 120 h (26.6%). The content of total phenolic compounds increased during the fermentation and was maximum after 96 h, which inhibited the DPPH radical by 87%. The promising characteristics of the protein and phenolic extracts of the biomass suggested the application in the coating composition for vegetal tissues...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein extract; Phenolic extract; Fermentation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600018

Cultivation and Biological Characterization of Chicken Primordial Germ Cells
Provedor de dados: 52
Autores: Ji,Meng; Guan,Wei-jun; Gao,Yu-hua; Li,Lu; Bai,Chun-yu; Ma,Yue-hui; Li,Xiang-chen.
The purpose of this work was to investigate the isolation, culture process of chicken gonadal primordial germ cells (PGCs) and study their biological characterization. PGCs were harvested from 5.5-day-old chicken embryonic genital ridges and explanted onto chicken embryonic fibroblasts (CEFs). The results showed that the primary cultivation of chicken PGCs on their own gonadal stroma cells were better than CEFs at first two days for reproduction. The conditioned media supported the growth and colony formation of PGCs for a prolonged time in vitro and maintained a normal diploid karyotype, which were positively stained by alkaline phosphatase (AKP), periodic acid Schiff (PAS) and reacted with anti-SSEA-1, SSEA-3, Oct4, Blimp1 and Sox2. Real-time PCR showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Primordial germ cells; Biological characteristics; In vitro.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100401

Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
Provedor de dados: 52
Autores: Hasbullah,Nor Azlina; Taha,Rosna Mat; Saleh,Azani; Mohamed,Normadiha.
In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gerbera jamesonii; Gamma irradiation; Callus; In vitro; Chlorophyll; Soluble protein.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300012

Genetic relationships among species of the genus Bothrops based on RAPD markers
Provedor de dados: 52
Autores: Grazziotin,Felipe; Echeverrigaray,Sergio.
The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; RAPD; Molecular markers; Genetic relationship.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005

Chemical compositon of three microalgae species for possible use in mariculture
Provedor de dados: 52
Autores: Moura Junior,Alfredo Matos; Bezerra Neto,Egídio; Koening,Maria Luise; Leça,Enide Eskinazi.
The purpose of this study was to determine the chemical composition of Chaetoceros gracilis, Cylindrotheca closterium and Tetraselmis gracilis, species frequently used as food for aquaculture in the northeast of Brazil. The species were grown in f/2 medium, in carboys, aerated from one liter, with constant illumination and analyses were made in late exponential growth phase. Cellular density (8,447 x 10 6 cell.L-1), soluble carbohydrates (0.10±0.01 mg.L-1) and magnesium (14.78±0.08 mg.L-1) were the highest in C. gracilis. C . closterium had the highest amount of chlorophyll a (13.76±1.11 mg.L-1), soluble proteins (0.62±0.04 mg.L-1), total amino acids (23.82±0.84 mg .L-1), nitrate (0.36±0.02 mg.L-1), sodium (0.46±0.05 mg.L-1) and phosphorus (2.80±0.38...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chaetoceros gracilis; Cylindrotheca closterium; Tetraselmis gracilis; Chemical composition.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300012

Erratum: Effect of LPS on the Viability and Proliferation of Human Oral and Esophageal Cancer Cell Lines
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300902

Activity of enzymes associated with the enzymatic browning of minimally processed potatoes
Provedor de dados: 52
Autores: Vitti,Maria Carolina Dario; Sasaki,Fabiana Fumi; Miguel,Patrícia; Kluge,Ricardo Alfredo; Moretti,Celso Luiz.
The purpose of the present study was to evaluate the effect of different potato cultivars and storage temperatures on the specific activity of phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) in minimally processed potatoes. Potato cultivars Agata, Asterix and Monalisa were selected, washed, peeled, diced, sanitized, centrifuged, vacuum- packed and stored at 5 and 15°C for 9 and 5 days, respectively. There was an increase in the enzymatic activity in all the cultivars stored at 15°C. The cultivars 'Agata' and 'Asterix' stored at 5ºC did not differ significantly between them for the PAL, PPO and POD activities. The PAL, PPO and POD activities were also influenced by the storage temperature. The cultivars Agata and Asterix...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum L; Phenylalanine ammonia-lyase; Polyphenol oxidase; Peroxidase; Minimal processing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500016

High molecular diversity of the fungus Guignardia citricarpa and Guignardia mangiferae and new primers for the diagnosis of the citrus black spot
Provedor de dados: 52
Autores: Stringari,Danyelle; Glienke,Chirlei; Christo,Daniel de; Maccheroni Jr.,Walter; Azevedo,João Lucio de.
RAPD markers were used to investigate the distribution of genetic variability among a group of Guignardia citricarpa, G. mangiferae, and Phyllosticta spinarum isolates obtained from several hosts in Brazil, Argentina, Mexico, Costa Rica, Thailand, Japan, United States and South Africa. Pathogenic isolates G. citricarpa Kiely (anamorph form P. citricarpa McAlp Van Der Aa) are the etiological agent of the Citrus Black Spot (CBS), a disease that affects several citric plants and causes substantial injuries to the appearance of their fruits, thus preventing their export. Several previous studies have demonstrated the existence of an endophytic species with high morphological similarity to the causal agent of CBS that could remain latent in the same hosts....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guignardia; SCARS; Citrus Black Spot; Citrus; Diagnosis; RAPD.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500002

Chromatographic characterization of products isolated from chrome shavings
Provedor de dados: 52
Autores: Garcia,Edivaldo E; Kimura,Cláudia C.M; Martins,Ariovaldo C; Rocha,Gisele O; Nozaki,Jorge.
Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chrome shavings; Enzyme; Chromatography; Protein; Chromium.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300003

Feasibility Study through Grid-Connected Photovoltaic Systems in Curitiba
Provedor de dados: 52
Autores: Silveira,Camila de Oliveira; Moreira,Allyson dos Reis; Moreira,Bárbara Luíza P.; Urbanetz Junior,Jair.
ABSTRACT The increase in energy demand in Brazil encourages the country to increasingly invest in generation of electric energy, where the demand for other renewable sources increases gradually because they have a lower impact on the environment. The use of Grid-Connected Photovoltaic Systems (GCPVS) is a viable solution for the country, since it presents favorable natural conditions for the use of solar energy. This study approaches the current scenario of three photovoltaic systems installed in Curitiba in 2016 and part of 2017: Green Office (GO) located in Curitiba Campus Downtown, Curitiba Campus Neoville, both of the Federal Technological University of Paraná (UTFPR), as well as a residence. By means of performance parameters, performance analysis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic Systems; Distributed Generation; Performance Parameters; Economic Viability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200219

Épocas de poda na cultura da mandioca na região noroeste do Paraná, Brasil
Provedor de dados: 52
Autores: Takahashi,Mário.
Este trabalho teve por objetivo avaliar o efeito da poda da parte aérea, realizada em três épocas distintas, sobre alguns parâmetros fitotécnicos da mandioca (Manihot esculenta Crantz). Todos os tratamentos foram plantados em outubro de 1991. Em 1992, efetuaram-se as podas da parte aérea em maio, julho e outubro. A testemunha foi conduzida sem poda. Em maio de 1993, todos os tratamentos foram colhidos. As produções da parte aérea na poda, foram alteradas pela quantidade de folhas. As produções fresca e seca das raízes e da parte aérea e o índice de colheita, foram modificados após a poda, em relação ao tratamento sem poda. O stand final e o número de raízes por planta, não foram alterados pela poda.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Cassava; Pruning; Stems.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400015

Histopathological Alterations in Common Carp (Cyprinus carpio L.) Gills Caused by Thiamethoxam
Provedor de dados: 52
Autores: Georgieva,Elenka; Stoyanova,Stela; Velcheva,Iliana; Yancheva,Vesela.
This work aimed to study the effect of new neonicotinoid thiamethoxam on the histological structure of common carp (Cyprinus carpio, L.) gills. Three experimental groups of fish were treated with 6.6, 10 and 20 mg/l thiamethoxam in laboratory conditions. Results showed different histological changes in the gill epithelium, which included lamellar lifting, edema, proliferation of the glandular cells and epithelium, covering the gill filament, fusion and degenerative alterations. The blood circulatory system showed mainly vasodilatation. All thiamethoxam concentrations activated compensatory-adaptive mechanisms, which caused pathological changes in the gills. Moreover, there was a tendency towards the enhancement of the gill histological changes, whose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecticides; Histopathology; Freshwater fish; Gills.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600991

Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
Provedor de dados: 52
Autores: Barry,Aline Fernandes; Alfieri,Alice Fernandes; Stipp,Danilo Tancler; Alfieri,Amauri Alcindo.
Neonatal diarrhea is an important cause of economic losses for cattle farmers. The main viral etiologies of enteric diseases are group A rotaviruses (GARV) and the bovine coronavirus (BCoV). Although both viruses infect calves of the same age, the occurrence of mixed infections is still under studied. The present study describes the co-infection of BCoV and GARV in stool samples. Forty-four diarrheic fecal samples from calves up to 60 days old that had previously tested positive for GARV by SS-PAGE were analyzed using semi-nested PCR for BCoV. A product with 251 bp of the BCoV nucleoprotein gene was amplified in 15.9% (7/44) of the samples, demonstrating that co-infection is not an unusual event. These results reinforce the need for testing for both GARV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Diarrhea; BCoV; Rotavirus; SN-PCR; Co-infection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700006

Pharmacological studies of Cordia salicifolia Cham in normal and diabetic rats
Provedor de dados: 52
Autores: Siqueira,Vera Lúcia Dias; Cortez,Diógenes Aparicio Garcia; Oliveira,Carlos Eduardo de; Nakamura,Celso Vataru; Bazotte,Roberto Barbosa.
The anti-obesity, appetite suppressant, diuretic and hipolipidemic effect of dried powdered leaves of Cordia salicifolia Cham (CS) were investigated. CS (20 mg.kg-1.day-1) dissolved in water was daily administered by gavage during 13 days. In contrast with the popular use as anti-obesity, appetite suppressant and diuretic, no effect were detected. However, hipolipidemic effect was observed in normal and alloxan-diabetic rats.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbal therapies; Hipolipidemic activity; Porangaba; Cordia salicifolia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300005

An outbreak of winter dysentery caused by bovine coronavirus in a high-production dairy cattle herd from a tropical country
Provedor de dados: 52
Autores: Takiuchi,Elisabete; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Filippsen,Patrícia; Alfieri,Amauri Alcindo.
Bovine coronavirus (BCoV) is a known cause of winter dysentery (WD) in adult cattle. The morbidity of the disease is high, that results in a significant decrease in milk production and consequently, economic losses. In the present study, we report on a classical outbreak of WD that affected a high-production Holstein dairy herd raised in a tropical country. The lactating batch included 154 cows, and 138 (90%) presented diarrhea in a short (nine days) period of time. Three (2%) cows died. The other batches of animals did not become ill. The evolution of the disease in the herd, including the clinical signs and epidemiological features, strongly suggested a WD case. Semi-nested PCR and RFLP confirmed that BCoV was the cause of the infection. Samples tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Dairy herd; Winter dysentery; Diarrhea; Bovine coronavirus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700008

Influence of the accumulation of phosphate and magnesium ions in the yeast cells on the ethanol productivity in batch ethanol fermentation
Provedor de dados: 52
Autores: Villen,Rafael Almud; Borzani,Walter; Netto,Antonio Sacco.
The accumulation of phosphate and magnesium in the yeast cells is not necessary to assure the ethanol productivity of batch ethanol fermentations. To avoid the decrease of the ethanol productivity it was sufficient to use a fermentation medium containing calculated concentrations of phosphorus and magnesium sources in order to maintain practically constant the phosphorus and magnesium initial contents of the biomass during the fermentation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Batch ethanol fermentation; Accumulation of phosphate and magnesium; Ethanol productivity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100020

The effect of intermittent cryotherapy on the activities of citrate synthase and lactate dehydrogenase in regenerating skeletal muscle
Provedor de dados: 52
Autores: Oliveira,Nuno Miguel Lopes de; Durigan,João Luiz Quagliotti; Munin,Flávia Simone; Schwantes,Maria Luiza Barcelos; Salvini,Tania de Fátima.
This study examined the effect of three sessions of cryotherapy (three sessions of 30 minutes applied each 2 h) and muscle compression in the regenerating skeletal muscle of the rats. The middle belly of tibialis anterior muscle was injured by a frozen iron bar and received one of the following intervention: injury + cryotherapy (treated with cryotherapy); injury + placebo (sand pack), and injury (I).The enzymatic activities of citrate synthase (CS) and lactate dehydrogenase (LDH) were measured in the presence of 1mM or 10mM pyruvate. The ANOVA and Tukey's test (p&lt;0.05) were performed for the statistical analysis. In summary, the intermittent sessions of cryotherapy, associated to muscle compression and applied immediately after the primary muscle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryotherapy; Muscle injury; Enzymatic analysis; Hypothermia; Tibialis anterior; Rehabilitation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100008

Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica)
Provedor de dados: 52
Autores: Llamoca-Zárate,Romulo Marino; Ponte,Luiz Ferreira Aguiar; Landsmann,Joerg; Campos,Francisco de Assis Paiva.
We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Opuntia ficus-indica; Prickly-pear; Cactaceae; Transient gene expression; Particle bombardment.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300005

Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
Provedor de dados: 52
Autores: Caron,Angelo Luis; Picanço-Castro,Virgínia; Ansorge,Sven; Covas,Dimas Tadeu; Kamen,Amine; Swiech,Kamilla.
ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Factor VIII; Hek293 cells; Lentiviral vectors; Polyethylenimine; Serum-free suspension culture; Transient transfection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923

Evaluation of probiotic properties of Pediococcus acidilactici B14 in association with Lactobacillus acidophilus ATCC 4356 for application in a soy based aerated symbiotic dessert
Provedor de dados: 52
Autores: Ribeiro,Maria Carolina de Oliveira; Vandenberghe,Luciana Porto de Souza; Spier,Michele Rigon; Paludo,Katia Sabrina; Soccol,Carlos Ricardo; Soccol,Vanete Thomaz.
The aim of this study was to evaluate the probiotic properties of Pediococcus acidilactici B14 and to study its resistance in the gastrointestinal system when combined with Lactobacillus acidophilus ATCC 4356 and used in a potentially symbiotic aerated soy based dessert. P. acidilactici B14 showed some important probiotic characteristics such as survival rate of 45.9% at pH 2.5; 72.4% in 0.3% bile salts and 95.8% after gastrointestinal transit at pH 4.0. Tolerance against the antibiotics cephalexin, neomycin, vancomycin, cefotaxime and penicillin G was also observed. The strain inhibited antagonism against the following cultures: Escherichia coli ATCC 25922, Bacillus cereus ATCC 33018, Staphylococcus aureus ATCC 6538P and Salmonella sp. The mixed culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediococcus acidilactici; Lactobacillus acidophilus; Gastrointestinal system; Probiotics; Soy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500755

Interannual and seasonal variability of macroinvertebrates in monsoonal climate streams
Provedor de dados: 52
Autores: Mesa,Leticia Mariana.
The aim of this study was to assess the interannual and seasonal changes in the community composition, abundance and presence-absence of macroinvertebrates in the streams of the northwestern of Argentina. The importance of environmental variability in determining these changes was also analyzed. Macroinvertebrates were collected in eleven streams during high (March) and low (September) water periods during two years. Physical variables and water parameters were also recorded at each site. Community composition and abundance differed significantly between seasons, but the presence-absence data did not exhibit a clear pattern of seasonal change. Rapid recolonization of disturbed substrate could ensure the recuperation of benthic community. Small body sizes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andean streams; Disturbance; Northwestern Argentina; Seasonal spates.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300011

Characterization of a Chrysodeixis includens nucleopolyhedrovirus Isolate from Brazilian Cerrado and Assessment of its Co-Infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus
Provedor de dados: 52
Autores: Sanches,Márcio Martinello; Sihler,William; Silva,Cláudia Efigênia Pereira; Guimarães,Giovana Curcio; Benito,Norton Polo; Sosa-Gómez,Daniel Ricardo; de Souza,Marlinda Lobo.
Abstract Chrysodeixis includens has become the major Lepidopteran pest of soybean crops, especially in the Brazilian Cerrado (savanna) region. A native isolate of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) from this region, Buritis, MG, was assessed for its biological and molecular features. In addition, in vitro co-infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), another virus of an important soybean pest, was tested. The ChinNPV-Buritis isolate presented an average LC50 of 7,750 occlusion bodies (OBs)/ml of diet in C. includens larvae. Analysis of restriction endonuclease profiles of viral DNA revealed similarities with previously described ChinNPV isolates IE, IF, and IG from Brazil, although the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgMNPV; Baculovirus; ChinNPV; Soybean looper; Velvetbean caterpillar.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100218

Phenolic Composition and Leishmanicidal Activity of Red Propolis and Dalbergia ecastaphyllum (L.) Taub (Fabaceae) Extracts from Sergipe, Brazil
Provedor de dados: 52
Autores: Araujo,Jaltaira Montalvão Etinger; Mendonça-Melo,Lucyana Santos; Araujo,Edilson Divino; Fernandes,Roberta Pereira Miranda; Scher,Ricardo.
ABSTRACT Leishmaniasis is a parasitic disease caused by protozoa of the Leishmania genus. It may manifest in visceral and tegumentary forms, and pentavalent antimonials are the first choice drugs used for the treatment. Frequently these drugs show low efficiency and high toxicity to mammalian host. The present study describes the chemical profile and the in vitro leishmanicidal effects of red propolis and Dalbergia ecastaphyllum extracts from Sergipe, Brazil, in Leishmania chagasi and Leishmania amazonensis promastigotes. The phenolic composition of the extracts was evaluated by direct infusion electrospray ionization mass spectrometry (ESI-MS) fingerprinting. The leishmanicidal effect was evaluated by the Resazurin colorimetric method. Similar composition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian red propolis; Chemical composition; Leishmania amazonensis; Leishmania chagasi.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100306

Detection of Specific Polypeptide(s) Synthesized during the Sequential Stages of Differentiation in Dioscorea species
Provedor de dados: 52
Autores: Kumar,Ashwani; Goyal,Suresh Chand; Dhansu,Pooja; Sheoran,Parvender; Kumar,Arvind; Verma,Ekta; Parshad,Jagdish; Singh,Anshuman.
ABSTRACTThe present investigation was aimed to detect the specific polypeptide(s) appeared during the sequential stages of differentiation. Among different explants, only nodal explants showed good results for callusing. Depending on the fresh and dry weight, best callus growth was observed on MS medium supplemented with NAA (2.5 mg/L) inDioscorea alata and 2, 4-D (2.0 mg/L) inD. deltoidea, respectively. This callus was used for the regeneration. Roots differentiation was observed on MS medium + NAA (2.0 mg/L) + IBA (0.5 mg/L) and shoots on MS medium + BAP (2.0 mg/L) + NAA (0.5 mg/L) in D. alata while in D. deltoidea, roots on RT medium + IAA (1.0 mg/L) and shoots on RT medium + BAP (1.0 mg/L) + NAA (0.5 mg/L). Continuous decrease was seen in the total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscorea alata; Dioscorea deltoidea; Callus; Differentiation; Polypeptides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500667

Spray Drying of Pequi Pulp: Process Performance and Physicochemical and Nutritional Properties of the Powdered Pulp
Provedor de dados: 52
Autores: Santana,Audirene; Kurozawa,Louise; Oliveira,Rafael; Park,Kil.
The objective of this work was to optimize the spray drying of pequi pulp using maltodextrin as carrier agent and Tween 80 as surfactant agent. A central composite rotatable design was used to evaluate the influence of inlet air temperature (140 to 200°C), maltodextrin (15 to 30%) and surfactant (0 to 5%) concentration on the process performance and physicochemical and nutritional properties of the dried powdered pulp. The dependent variables were process yield (27.4 - 51.7%), outlet air temperature (106.5 - 135°C), energetic efficiency (29.9 - 44.8%), moisture content (0.25 - 1.43%), water activity (0.09 to 0.21), hygroscopicity (9.1 - 12.1 g adsorbed moisture/100g dry matter), vitamin C content (129.8 - 303.0 mg/g solids pequi) and total carotenoids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fruit; Tween 80; Powder property; Screening design; Microscopy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100500

Extraction time for soybean isoflavone determination
Provedor de dados: 52
Autores: Carrão-Panizzi,Mercedes C.; Favoni,Silvana Pedroso de Goés; Kikuchi,Akio.
Studies were carried out on the optimization of the extraction procedures for isoflavones determination in soybean by HPLC. Isoflavones were extracted from 100 mg samples of full fat soybean flour "Kinako" (milled toasted grains). Recovery of average total isoflavones was higher when extraction was performed with agitation(217.2 mg/100g), than without agitation(191.0 mg/100g). Isoflavone extraction without agitation for 1,4, 20 and 24 hours, were equally efficient for total and individual compounds. These results suggested that an efficient isoflavone recovery could be achieved with extraction for one hour with agitation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybeans; Isoflavones; Glucosides; Aglucones; Extraction; Ethanol; HPLC.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600015

Psycological and physiological responses to stress: a review based on results from PET and MRI studies
Provedor de dados: 52
Autores: Cortez,Célia Martins; Cruz,Frederico Alan de Oliveira; Silva,Dilson.
A new application for the nuclear imaging techniques is the study of organic responses to stress. Neuroimaging techniques allow the assessment of brain activation changes in association with the metabolic responses to stress. In this paper, a review of general effects of the stress on organic activity is made, emphasizing important advances introduced by studies using PET and fMRI. The importance of the hypothalamus-pituitary-adrenal axis to onset the adequate psychical and organic responses to sustain the homeostasis during the stress is discussed, as well as the possibility of traumatic stressing experiences have negative effects on the brain.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; Nuclear imaging; Physiological responses.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700003

Brachyura (Crustacea, Decapoda) associated to Schizoporella unicornis (Bryozoa, Gymnolaemata) in Ubatuba bay (SP), Brazil
Provedor de dados: 52
Autores: Mantelatto,Fernando L. M.; Souza-Carey,Marisa M.
This work characterizes the composition of Brachyura from Schizoporella unicornis. The samples were collected in 1995 at Itaguá Beach, Ubatuba (SP), at three month intervals, during all seasons from January to December. The Bryozoa colonies were obtained by snorkeling at a depth of five meters in daylight. A total of 323 specimens were collected from four families (Xanthidae, Portunidae, Majidae and Grapsidae). Pachygrapsus transversus, Hexapanopeus schimitti, and Menippe nodifrons occurred in all seasons. The highest and lowest number of individuals occurred during the spring and autumn, respectively. Xanthidae exhibited the highest density during the summer, autumn, and spring, while Grapsidae exhibited the highest density in winter. It was noted the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachyuran; Bryozoan; Check list; Crabs; Schyzoporella.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200007

Evaluation of the Folin-Ciocalteu Method and Quantification of Total Tannins in Stem Barks and Pods from Libidibia ferrea (Mart. ex Tul) L. P. Queiroz
Provedor de dados: 52
Autores: Galvão,Marcos Aurelio Morais; Arruda,Alice Oliveira de; Bezerra,Isabelle Cristinne Ferraz; Ferreira,Magda Rhayanny Assunção; Soares,Luiz Alberto Lira.
ABSTRACT The stem bark and pods of “jucá” are widely used in Brazilian folk medicine and, despite their therapeutic relevancies, there are insufficient reports about their quality control. Both herbal drugs from the species are rich in tannins, which showed a lot of biological and economic interest. Thus, the purpose of this study was to evaluate the method for quantification of tannins in the stem bark and pods from L. ferrea. The performance of the method was tested against the influence of the most relevant parameters (reaction time; amount of drug; type and amount of precipitationagents; and, concentration of the reagents). The procedure was validated according to the literature. After optimization, the experimental conditions were established as: 30...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Libidibia ferrea; Quality control; Spectrophotometry; Tannins.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100431

An aqueous extract of Vitex agnus castus alters the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Costa,Maria Regina de Macedo; Ribeiro,Camila Godinho; Santos-Filho,Sebastião David; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida.
The development of experimental assays to study properties of herbal medicine is worthwhile. Vitex agnus castus (VAC) is utilized in popular medicine and some actions have been attributed to its extract. Blood cells (BC) and plasma proteins are labeled with technetium-99m (Tc-99m) and have been used in nuclear medicine, as in basic research. This procedure uses a reducing agent and stannous ion is utilized. There are reports that drugs can alter this labeling process. The aim of this work was to evaluate the influence of an aqueous extract of VAC on the labeling of blood constituents with Tc-99m. Blood was incubated with VAC, stannous chloride and Tc-99m, as sodium pertechnetate, and centrifuged. Samples of BC and plasma were separated, aliquots of BC and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidant properties; Plasma proteins; Red blood cells; Technetium-99m; Vitex agnus-castus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600023

Assessment of genetic diversity in Cattleya intermedia Lindl. (Orchidaceae)
Provedor de dados: 52
Autores: Machado Neto,Nelson Barbosa; Vieira,Luiz Gonzaga Esteves.
Orchids are valuable pot plants and Cattleya intermedia is a promising species underused in breeding programs. Recently, breeding work with this species produced superior plants that are believed to be not the true species owing to the morphological differences from wild plants. The aim of this study was to estimate the level of genetic diversity and interrelationships between wild and bred Cattleya intermedia collected at three different Brazilian states and from commercial breeders with RAPD markers. A total of 65 polymorphic bands were used to generate a genetic distance matrix. No specific groupings were revealed by the cluster analysis as bred materials were not different from wild plants. The genetic differentiation (F ST = 0.01626) was very low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fst; Domestication molecular markers genetic variability orchids.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500011

Comparação do peso dos quelípodos e crescimento em duas espécies de "sirís" do gênero Callinectes (Brachyura, Portunidae)
Provedor de dados: 52
Autores: Guerrero-Ocampo,Cecilia Margarita; Negreiros-Fransozo,Maria Lucia; Costa,Tânia Marcia.
Neste trabalho, as espécies C. danae e C. ornatus foram comparadas quanto ao peso dos quelípodos. Os siris foram coletados com 2 redes de arrasto do tipo "otter trawl" na Enseada de Ubatuba (23° 26' S e 45° 02' W). As constantes alométricas obtidas foram analisadas por meio da função potência (Y = aXb ). As espécies apresentaram diferentes graus alométricos para cada sexo. A relação PQ X PC apresentou alometria positiva em ambas espécies, porém os machos apresentam maior alometria positiva do que as fêmeas. C. danae apresenta maior alometria positiva para os quelípodos do que C. ornatus. O fato de C. danae atingir maior porte e possuir quelípodos maiores indica que esta espécie tem potencial para o cultivo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Siris; Quelípodo; Crescimento relativo.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400013

A comparison of efficiency parameters of SSR markers and genetic diversity analysis in Amorphophallus paeoniifolius (Dennst.) Nicolson
Provedor de dados: 52
Autores: Mandal,Rupsanatan; Nag,Sumita; Tarafdar,Jayanta; Mitra,Surajit.
ABSTRACT Amorphophallus (elephant foot yam) is an herbaceous edible aroid crop which belongs to the family Araceae. The study was undertaken to identify the efficient SSR primer that could differentiate a set of 12 elephant foot yam genotypes. Various efficiency parameters, namely, Polymorphism Information Content (PIC), Marker Index (MI), Resolving Power (RP) and Diversity Index (DI) were studied for 11 primers. The relationship between the parameters was studied using Spearman rank correlation coefficient. Discrimination analysis was done to find out the most effective parameter. Finally Principal Coordinate Analysis (PCoA) and dendrogram was done to find out the genetic diversity among the germplasm. The SSR markers under this investigation will...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elephant foot yam; Marker Index; Discrimination analysis and Principal coordinate analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100436

Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997
Provedor de dados: 52
Autores: Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko.
Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006

Ozone: an emerging technology for the seafood industry
Provedor de dados: 52
Autores: Gonçalves,Alex Augusto.
In recent years, increasing attention has been focused on the safety of foods, and in particular on the intervention methods to reduce and eliminate human pathogens from fresh product, especially fresh seafood. Traditional technology utilizes water with or without a sanitizing agent to wash fresh seafood. Chlorine is the most widely used sanitizing agent available for fresh product, but it has a limited effect in killing bacteria on seafood surfaces. An alternative treatment is being sought to improve food safety. Many research and industrial trials are underway to validate the use of ozone in the food industry. This article intends to show a clean technology to be applied in seafood industry and to show that many studies must be done to demonstrate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clean technology; Ozone; Seafood.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600025

Treatment of simulated wastewater containing n-phenyl-n-isopropyl-p-phenylenediamine using electrolysis system with Ti/TiRuO2 electrodes
Provedor de dados: 52
Autores: Inazaki,Thelma Helena; Pião,Antonio Carlos Simões; Bidoia,Ederio Dino.
This study investigated the effects of the electrolytic treatment in the simulated wastewater with aromatic amine n-phenyl-n-isopropyl-p-phenylenediamine (Flexzone 3P®) using Ti/TiRuO2 electrodes under 0.025 A/cm² (DC) for different electrolysis durations (5; 15; 30; 45 and 60 min). Conductivity, pH, UV-visible spectra, gas chromatograms, toxicity and biodegradation tests were carried out. During the electrolytic treatment the pH decreased and conductivity increased slightly. After 60 min of electrolysis, the concentration of Flexzone 3P decreased by 65.1%. UV-vis spectra and chromatograms of simulated wastewater showed changes in the molecular structure of the aromatic amine. After 5 and 15 min of electrolysis, the simulated wastewater containing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrolytic treatment; Wastewater; Ti/TiRuO2 electrodes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600018

Advances in bacterial specific imaging
Provedor de dados: 52
Autores: Wareham,David; Michael,J.; Das,Satya.
Nuclear medicine is a powerful diagnostic technique able to detect inflammatory foci in human disease. A wide range of agents have been evaluated for their ability to distinguish lesions due to microbial infection from those due to sterile inflammation. Advances continue to be made on the use of radiolabelled antibiotics which as well as being highly specific in the diagnosis of infection may be useful in monitoring the treatment and course of disease. Here we provide an update on in-vitro and clinical studies with a number of established and novel radiopharmaceuticals
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Infection specific imaging; Radiolabelled anti-infectives; 99mTc- ciprofloxacin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700021

Evaluation of the effect of an extract of sabugueiro (Sambucus australis) on the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Ribeiro,Camila Godinho; Rebello,Bernardo Machado; Neves,Rosane de Figueiredo; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida.
Sambucus australis (sabugueiro) has been used to treat inflammatory and rheumatologic disorders. Blood constituents labeled with technetium-99m (99mTc) have been used in nuclear medicine to obtain diagnostic images. The aim of this work was to evaluate the effect of a sabugueiro extract on the labeling of blood cells with 99mTc. Blood samples from Wistar rats were incubated with sabugueiro extract and the radiolabeling assay of blood constituents was carried out. After centrifugation, samples of plasma and blood cells were separated. Aliquots of plasma and blood cells were precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions. The radioactivity in each fraction was counted and the percentage of activity (%ATI)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sambucus australis; Blood constituents; Technetium-99m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600020

Microrganismos eficazes na produção da cultura do feijoeiro
Provedor de dados: 52
Autores: Ronzelli Júnior,Pedro; Buff,Marly Terezinha Coradassi; Koehler,Henrique Soares.
Em razão da importância do feijão como alimento e da necessidade de produzir mais dessa fabácea (leguminosa) foram conduzidos dois experimentos, nos anos agrícolas de 1995/96 e 1996/97, para avaliar a ação fertilizante do produto microrganismos eficazes (EM) em comparação com a adubação química NPK tradicional. O trabalho experimental foi realizado na Universidade Federal do Paraná - Estação Experimental do Canguiri, em Pinhais, PR, em área uniforme com solo caracterizado como Latossolo Vermelho Amarelo Álico. O preparo do solo foi o convencional com aração e gradagens. O EM foi testado em seis diluições, 1:250, 1:500, 1:750, 1:1.000, 1:1.250 e 1:1.500. As aplicações do produto foram sempre sobre as folhas das plantas e em sete épocas, para todas as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Efficient microorganism; Yield.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400014

Endophytic Fungi as Novel Resources of natural Therapeutics
Provedor de dados: 52
Autores: Rajamanikyam,Maheshwari; Vadlapudi,Varahalarao; amanchy,Ramars; Upadhyayula,Suryanarayana Murty.
ABSTRACT Fungal endophytes constitute a major part of the unexplored fungal diversity. Endophytic fungi (EF) are an important source for novel, potential and active metabolites. Plant-endophyte interaction and endophyte -endophyte interactions study provide insights into mutualism and metabolite production by fungi. Bioactive compounds produced by endophytes main function are helping the host plants to resist external biotic and abiotic stress, which benefit the host survival in return. These organisms mainly consist of members of the Ascomycota, Basidiomycota, Zygomycota and Oomycota. Recently, the genome sequencing technology has emerged as one of the most efficient tools that can provide whole information of a genome in a small period of time....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic fungi; Ascomycota; Natural products; Antidiabetic.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100430

L(+)-Lactic acid recovery from cassava bagasse based fermented medium using anion exchange resins
Provedor de dados: 52
Autores: John,Rojan P.; Nampoothiri,K. Madhavan; Pandey,Ashok.
The properties of the ion exchange resins, Amberlite IRA 402, a strong anion exchange resin and IRA 67, a weak anion exchange resin were determined to evaluate their comparative suitability for lactic acid recovery from fermented cassava bagasse. Data on binding capacities and recovery proved that weak base resin in chloride form was the most favourable ones for lactic acid recovery from aqueous solutions and fermentation media. Fermented media obtained through simultaneous saccharification and fermentation of cassava bagasse starch hydrolysate based medium were used for lactic acid recovery study using weak base resin column. Amberlite IRA 67 had much more efficiency than Amberlite IRA 402 to recover lactic acid. Like in other reports, due to the presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L(+) lactic acid; Lactobacilli; Anion exchage resin; Cassava bagasse.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600020

Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling
Provedor de dados: 52
Autores: Cardoso,Milena Barcelos; Kaltchuk-Santos,Eliane; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.
Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; FDA; Binucleate pollen grains; Multinucleate pollen grains; Spontaneous diploidization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005

A model for the study of skeletal anomalies in rat fetuses
Provedor de dados: 52
Autores: Effting,Cristiane; Paula,Daniel Jesus de; Nunes Junior,Guilhermino Pereira.
The aim of this study was to validate a model of skeletal anomalies in rat fetuses by the administration of ketoconazole (80 mg/kg) to pregnant rats during organogenesis. Bones of the head, trunk and anterior and posterior limbs were examined for detection of anomalies. Statistical differences regarding the number of fetuses and postimplantation resorptions, and fetal and placental weight were significant. The frequency of skeletal anomalies in the head, trunk, and anterior and posterior limbs in the ketoconazole-treated group were also significant when compared to the control group. It could be concluded that the model suggested was valid for study of skeletal anomalies and abnormal bones development in rat fetuse, in spite of the loss of fetuses due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azol Antifungals; Ketoconazole; Models; Pregnancy; Skeletal Malformation; Steroids; Teratogenesis; Wistar Rats.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100005

Exosomes as Biomarker of Cancer
Provedor de dados: 52
Autores: Sumrin,Aleena; Moazzam,Shumaila; Khan,Aleena Ahmad; Ramzan,Irsa; Batool,Zunaira; Kaleem,Sana; Ali,Moazzam; Bashir,Hamid; Bilal,Muhammad.
ABSTRACT Rapid advances in medicine and biotechnology resulted in the development of non-invasive diagnostic and prognostic biomarkers enabling convenient and accurate detection. Exosomes has recently emerged as non-invasive biomarker for a number of diseases including cancer. Exosomes are the small endosome originated membranous vesicles secreted in a number of biological fluids such as serum, saliva, urine, ascites, cerebrospinal fluid, etc. Exosomes contain microRNA proteins and mRNA which can be used as disease specific biomarkers. Here we reviewed recent advancement in the field of exosomes as diagnostic biomarker for cancer along with a brief overview of their biogenesis, function and isolation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exosome; Biomarker; Cancer biomarker.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100309

Combining a probiotic with organic salts presents synergistic in vitro inhibition against aquaculture bacterial pathogens
Provedor de dados: 52
Autores: Bolivar,Norha Constanza; Legarda,Esmeralda Chamorro; Seiffert,Walter Quadros; Andreatta,Edemar Roberto; Vieira,Felipe do Nascimento.
ABSTRACT This study aimed to evaluate 1) the in vitro effect of organic salts on the growth of the probiotic Lactobacillus plantarum and then 2) the combined use of a probiotic with organic salts on the in vitro inhibition of V. alginolyticus, A. hydrophila, E. coli, P. aeruginosa, and S. agalactiae. In vitro tests were performed with eight different organic salts, including butyrate, propionate, succinate, citrate, formate, fumarate, glutamate, and acetate, at two pH values (6.2 and 7.1) to determine their effect on the growth kinetics of L. plantarum. In addition, each organic salt was tested alone and in combination with L. plantarum to evaluate the inhibitory effect against the pathogenic bacteria noted above in either condition. Sodium citrate and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus plantarum; Sodium butyrate; Sodium propionate; Aquaculture pathogenic bacteria.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100415

Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3
Provedor de dados: 52
Autores: Isil,Seyis; Nilufer,Aksoz.
In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were investigated. It was concluded that for maximum xylanase activity, incubation at 60°C in an enzyme incubation medium with pH 5 that contained 1 % xylan was appropriate. The stability studies showed that the enzyme was relatively stable in the pH range 3-7 and retained more than 50 % of its original activity after four months.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma harzianum; Xylan; Xylanase; Lignocellulosic material; Enzyme stability; Metal ions.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200004

The Photovoltaic Generation and its Energy Contribution and Demand Shifting at the Center Headquarters of the Federal University of Technology - Paraná - Campus Curitiba
Provedor de dados: 52
Autores: Mariano,Juliana D’Angela; Urbanetz Junior,Jair.
ABSTRACT Distributed photovoltaic (PV) generation is characterized by the application of several small power plants in urban centers. This form of energy deserves special mention due to the possibility of installation in existing areas such as roofs and facades. Thus, the implementation of these systems represents positive modifications of the urban scenario, with the adhesion of PV modules, presenting much smaller social and environmental impacts than that of large conventional plants. In this sense, this study aims at analyzing the demand and consumption curves of the Center headquarters buildings of Federal University of Technology - Paraná (UTFPR) in Curitiba, by applying the COPEL’s CAS Hemera platform, in order to determine the potential for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distributed generation; Photovoltaic generation curves; Solar irradiation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200227

Morphology and anatomy of the Cordia trichotoma (Vell.) Arrab. ex I. M. Johnst diaspore (Boraginaceae)
Provedor de dados: 52
Autores: Souza,Luiz Antonio de.
This study analyzed the morphology and anatomy of the Cordia trichotoma (Vell.) Arrab. ex I. M. Johnst diaspore. The objectives were: the structural analysis of the calyx and corolla that persisted in the ripe fruit and acted in the anemocoric dispersion process; the vascularization of the gynoecium for better understanding of the carpel/locule number of the ovary; nectary structure; and the anatomical analysis of the developmental pericarp and seed for better definition of the fruit type. The results showed that the perianth had aerenchyma and the ovary was 2- 4- locule forming endothelium and false septum. The fruit was drupoid nuculanium and the seed was exotestal.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Perianth; Nectary; Fruit; Seed.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400014

Hydrolysis of insoluble fish protein residue from whitemouth croaker (Micropogonias furnieri) by fungi
Provedor de dados: 52
Autores: Martins,Vilásia Guimarães; Palezi,Simone Canabarro; Costa,Jorge Alberto Vieira; Prentice,Carlos.
A significant amount of insoluble fibrous protein, in the form of feather, hair, scales, skin and others are available as co-products of agro industrial processing. These wastes are rich in keratin and collagen. This study evaluated different fungi for the hydrolysis of insoluble fish protein residues. Proteins resulting from Micropogonias furnieri wastes through pH-shifting process were dried and milled for fermentation for 96 h. This resulted the production of keratinolytic enzymes in the medium. Trichoderma sp. on alkaline substrate (28.99 U mL-1) and Penicillium sp. on acidic substrate (31.20 U mL-1) showed the highest proteolytic activities. Penicillium sp. showed the largest free amino acid solubilization (0.146 mg mL-1) and Fusarium sp. the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish waste; Fungi; Fibrous proteins; Hydrolysis; Whitemouth croaker.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100014

A new enzymatic process for the treatment of phenolic pollutants
Provedor de dados: 52
Autores: Palma,Mauri Sergio Alves; Horn,Harald; Zilli,Mario; Pigatto,Gisele; Converti,Attilio.
This work aimed to develop a new economic enzymatic process to treat the phenolic pollutants using pure tyrosinase in stirred vessel and adopting temperature (T), pH, rotational speed (N), initial phenol (C P,o) and enzyme (C T) concentrations as independent variables. Experimental data of the residual phenol concentration (C P) were used to calculate the oxidation efficiency (η), initial oxidation rate (-r o) and time required to reach the end of reaction (t) that were selected as the responses. Under the optimal conditions (T = 45°C, pH 6.6, N = 400 rpm, C P,o = 100 ppm and C T = 50 U/mL), η was 88.1%, -r o = 10.2 mg L-1 min-1, t = 40 min. These results suggested that tyrosinase-rich crude extracts from vegetable byproducts could be quite promising.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenols; Tyrosinase; Wastes; Enzymatic treatment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400016

Sperm depletion: a cost for single mated females of Podisus nigrispinus (Dallas, 1851) (Heteroptera: Pentatomidae)
Provedor de dados: 52
Autores: Sousa-Souto,Leandro; Evangelista Júnior,Walter Santos; Lima,Eraldo Rodrigues; Zanuncio,José Cola; Fonseca,Marcy das Graças.
The aim of this work was to test if egg viability of polyandrous females was increased with increasing number of matings. Longevity and reproductive output of females of the predatory stinkbug Podisus nigrispinus that were allowed to mate for 0, 1, 2, 3 times or were in the continuous presence of the same male was evaluated. Polyandry resulted in diminished sperm depletion. Females that had mated three times or that were in continuous presence of a male produced more offspring than females that mated once or twice throughout their lifetime. There was a negative correlation of mating history on female longevity. Results indicated that remating, either with same male or with different males were crucial for maximization of the reproductive success of females.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asopinae; Multiple matings; Reproductive success; Biological control; Mate choice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700009

Growth characteristics modeling of Lactobacillus acidophilus using RSM and ANN
Provedor de dados: 52
Autores: Meena,Ganga Sahay; Kumar,Nitin; Majumdar,Gautam Chandra; Banerjee,Rintu; Meena,Pankaj Kumar; Yadav,Vijesh.
The culture conditions viz. additional carbon and nitrogen content, inoculum size, age, temperature and pH of Lactobacillus acidophilus were optimized using response surface methodology (RSM) and artificial neural network (ANN). Kinetic growth models were fitted to cultivations from a Box-Behnken Design (BBD) design experiments for different variables. This concept of combining the optimization and modeling presented different optimal conditions for L. acidophilus growth from their original optimization study. Through these statistical tools, the product yield (cell mass) of L. acidophilus was increased. Regression coefficients (R²) of both the statistical tools predicted that ANN was better than RSM and the regression equation was solved with the help of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Response surface methodology (RSM); Artificial neural network (ANN); Genetic algorithms (GA); Box-behnken besign (BBD).
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100003

Caloric variability of Corbicula fluminea (Mollusca, Bivalvia) in Rosana Reservoir, Brazil
Provedor de dados: 52
Autores: Bagatini,Yara Moretto; Benedito-Cecilio,Evanilde; Higuti,Janet.
The purpose of the present study was to investigate temporal and longitudinal variation of caloric density of Corbicula fluminea in Rosana Reservoir. Significant differences concerning the caloric density of C. fluminea were observed throughout the year at the three reservoir sites. The caloric density variation during the year was probably related to reproductive activity, which demanded higher allocation of energy in certain period. The food source used by this species might be an important factor in the caloric value temporal and spatial variation of C. fluminea in Rosana Reservoir.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. fluminea; Caloric content; Temporal and longitudinal variation; Reservoir.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100010

The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
Provedor de dados: 52
Autores: Lima,Francisco Esmaile de Sales; Batista,Helena Beatriz Ruthner; Campos,Fabrício Souza; Kunert Filho,Hiran Castagnino; Cibulski,Samuel; Finoketti,Fernando; Arantes,Thalita; Spilki,Fernando; Roehe,Paulo Michel; Rijsewijk,Franciscus Antonius Maria; Franco,Ana Cláudia.
This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-5; V mRNA; Transfection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007

Production of spores of Trichoderma harzianum on sugar cane molasses and bagasse pith in solid state fermentation for biocontrol
Provedor de dados: 52
Autores: Rodríguez-León,Jose A.; Domenech,F.; León,M.; Méndez,T.; Rodríguez,D. E.; Pandey,Ashok.
Solid state fermentation was carried out for the production of spores from Trichoderma harzianum No 53 using sugar cane bagasse pith as solid matrix and sugar cane molasses as carbon and energy source. Different nitrogen sources such as urea, (NH4)2SO4 , NH4H2PO4 and (NH4)2HPO4 were added in the media to test their effect on spores production. Among these, urea was found most useful that resulted high no of spores (1x10(9)/gDM). The influence of temperature and initial moisture of the substrate was studied through a 2² experimental plan design. No statistical differences were found within the range of 30-35ºC and 60-70% for temperature and moisture respectively. The biotechnological parameters of the process were derived from the Oxygen Uptake Rate (OUR)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma harzianum; Solid state fermentation; Sugar cane pith and molasses; Biocontrol; Kinetics.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100010

Use of Coffee Capsules as Support Material in Upflow Anaerobic Fixed Bed Reactors
Provedor de dados: 52
Autores: Souza,Gabriela Rezende de; Oliveira,Luiz Fernando Coutinho de; Bello,Italoema Pinheiro; Siniscalchi,Luciene Alves Batista; Fia,Ronaldo; Gandia,Rômulo Marçal.
Abstract The aim of this work is to evaluate the performance of upflow anaerobic fixed bed reactors filled with espresso coffee capsules to treat sanitary sewage. Three reactors (R1, R2 and R3) were constructed in blue PVC pipes measuring 30 cm height and 150 mm diameter and filled with coffee capsules made of aluminum and plastic. The sewage from the pre-treatment phase of the wastewater treatment plant of the Federal University of Lavras fed the system. Temperature, pH, alkalinity and volatile acids concentration, COD, TS, TVS and TSS of the influent and effluent were analyzed to evaluate the reactors performances. Statistics tests were run in the software Statistica 10. Changes occurred in the organic loading rates caused two different operating phases,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaerobic filter; Biomass; Immobilization; Support material; Domestic sewage.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100223

Quantificação das substâncias fenólicas totais em órgãos da aroeira Schinus Terebenthifolius (RADDI)
Provedor de dados: 52
Autores: Queires,Luís Carlos Soares; Rodrigues,Luiz Erlon Araujo.
The "aroeira" (Schinus terebenthifolius, RADDI), known as a toxic plant is actually very used in popular medicine, mainly in the treatment of general inflammatory processes. Many of its properties or of its curative effects may be attributed to the different polyphenols that belong to its structural constitution. In this work we have demonstrated that the distribution of total phenols was different in the several organs of the "aroeira", such as leaves, flowers, fruits and stem. The importance of these substances for the plant physiology and defense mechanisms, their ecological relationships and some therapeutic uses were also discussed. The correlation between the polyphenol properties and the metabolism of animal cells, suggest special attention when the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic substances; Aroeira; Schinus terebenthifolius.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200012

Biochemical and Utralmicroscopic Evaluation of Myofibril Proteins and Collagen during Ageing in Broiler Chicken PSE (Pale, Sof, Exudative) Meat
Provedor de dados: 52
Autores: Marchi,Denis Fabrício; Beteto,Flávia Maria; Almeida,Gleice Rocha dos Santos; Soares,Adriana Lourenço; Hernandez-Blazquez,Francisco Javier; Ida,Elza Iouko; Shimokomaki,Massami.
Abstract The aim of this work was to study the myofibril proteins and collagen fraction changes in broiler chickens PSE (pale, soft, exudative) meat during ageing and their relationship to meat quality. The results presented an increase of myofibril proteins and collagen solubility promoted by the enhanced proteases activities during storage. Ultramicroscopically, the PSE meat samples revealed intracellularly a sarcomere super contraction and lacunas within the A and I bands while Z-lines appeared very dense and fragmented in comparison to normal samples. This observation was noticed already at 4h storage while extracellularly collagen fibrils decreased visually within the endomysium only after 24h of conditioning. These results influenced the quality as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cooking loss; Tenderness; Collagen content; Myofibrillar Fragmentation Index.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100503

Development of an enzyme immunoassay for poliovirus antigens
Provedor de dados: 52
Autores: Hashimoto,Newton; Benati,Fabrício José; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Nozawa,Carlos Mitihiko.
An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme immunoassay; Poliovirus antigen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005

Isolation of alkalophilic CGTase-producing bacteria and characterization of cyclodextrin-glycosyltransferase
Provedor de dados: 52
Autores: Higuti,lma Hiroko; Grande,Simone Wichert; Sacco,Roberta; Nascimento,Aguinaldo José do.
One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75 strains were isolated that produced a yellowish halo surrounding the colonies, due to a phenolphtalein-cyclodextrin (CD) complex, and these were selected as alkalophilic CGTase-producing bacteria. All the 75 strains were identified as Bacillus firmus by microscopy and biochemical tests. The activity of the CGTase's varied from 2² to 2(10) dilutions,when assayed by CD-trichloroethylene (TCE)-complex precipitation. Strain 31 that produced the enzyme at the higher level was selected, and its enzyme was partially purified by starch adsorption (x 17) in a yield of 51%. Maximum enzyme activity occurred at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Characterization.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200007

Lead behavior in soil treated with contaminated sewage sludge and cultivated with maize
Provedor de dados: 52
Autores: Barriquelo,Marcilene Ferrari; Juliatti,Marinês Aparecida; Silva,Margarete Soares da; Lenzi,Ervim.
Lead (Pb) behavior was studied in soil treated twice with sewage sludge contaminated with lead at interval of 18 months. Soil samples received five different treatments: three with lead [0 (reference); 2,500; 5,000 mug g-1] and two with a mixture of lead and cadmium (interferer) (2,500 + 2,500 and 5,000 + 5,000 mug g-1, respectively). Vases containing treated soil were cultivated with maize in a greenhouse for 75 days. Pb was extracted using nitric perchloric digestion and analyzed by atomic absorption spectroscopy. The soil, containing sewage sludge contaminated by lead presented a higher concentration of Pb. The metal concentration remained constant in all treatments at 20-60 cm depth. No absorption of Pb by the plants was detected.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sewage sludge; Heavy metal; Lead; Environmental pollution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400002

Diversity of Endophytic and Epiphytic Bacteria From Sugarcane in Khuzestan, Iran
Provedor de dados: 52
Autores: Rezamahalleh,Hossein Moazzen; Khodakaramian,Gholam; Hassanzadeh,Nader.
Abstract Diverse microorganisms are living as endophytes in plant tissues and as epiphytes on plant surfaces in nature. Commercial formulations of bacteria antagonist to plant pathogenic microbes and ice nucleation active bacteria have been utilized as an environmentally safe method to manage plant disease and to prevent frost damage respectively. Bacteria were isolated from the leaf and sheath of sugarcane (CP69-1026 CP57-614, CP48-103, CP73-21, and CP70-1143 cultivars) verities grown in the ﬁeld in Khuzestan province, Iran. Bacteria were found in both sheaths and leaves of sugarcane plants which they were signiﬁcantly higher in density in leaves and which most were endophytic. The bacterial strains were 10 groups on the basis of the biochemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rRNA; Diversity; Endophytic bacteria; Sugarcane; Khuzestan.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100429

Reproductive pattern comparison of Uca thayeri Rathbun, 1900 and U. uruguayensis Nobili, 1901 (Crustacea, Decapoda, Ocypodidae)
Provedor de dados: 52
Autores: Costa,Tânia Marcia; Silva,Stella Maris Januário; Negreiros-Fransozo,Maria Lucia.
Ovigerous females of Uca thayeri and U. uruguayensis were used to achieve and compare their reproductive pattern. The females mean size was 6.6 ± 0.8mm for U. uruguayensis and 17.3± 4.4mm for U. thayeri. The mean fecundity obtained for U. uruguayensis was 1883 ± 490 eggs (N = 27) and for U. thayeri was 31068 ± 11186 eggs (N = 31). The differences observed in reproductive pattern of U. thayeri and U. uruguayensis suggest an adaptive strategy by each species: U. thayeri presented seasonal reproduction with a pronounced intensity from January to March and higher fecundity, while U. uruguayensis reproduction was continuous all year round, but with lower fecundity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea; Fiddler crab; Fecundity; Uca thayeri; U. uruguayensis; Reproductive pattern.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100014

Screening of native yeast from Agave duranguensis fermentation for isoamyl acetate production
Provedor de dados: 52
Autores: Hernández-Carbajal,Gerardo; Rutiaga-Quiñones,Olga Miriam; Pérez-Silva,Araceli; Saucedo-Castañeda,Gerardo; Medeiros,Adriane; Soccol,Carlos Ricardo; Soto-Cruz,Nicolás Óscar.
In this work, fifty yeast strains, isolated from the spontaneous alcoholic fermentation of Agave duranguensis to produce mezcal, were tested using the double coupling system. These yeasts were from the genera Pichia, Torulaspora, Saccharomyces, Kluyveromyces, Deckera, Hanseniaspora, and Candida. P. fermentans ITD00165 was the best isoamyl acetate producer, yielding 0.38 g/L of ester after incubation for 24 h, while K. marxianus ITD00211 produced 0.32 g/L of ester. Thus P. fermentans TD00165 could be considered as an excellent choice for use in optimization studies of the culture medium and bioreactor operating conditions to develop a process for biotechnological production of isoamyl acetate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Banana aroma; Food additive; Native yeast strains.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300002

Analysis of solar photovoltaic energy potential in Brazilian hydroelectric reservoirs through floating panels
Provedor de dados: 52
Autores: Santos,Francielle da Rocha; Mariano,Juliana D’Angela; Sestrem Junior,Jonas Abilio; Urbanetz Junior,Jair.
Abstract Sustainability, a concept used to exploit natural resources without harming future generations, is being applied to power generation. In Brazil, the main source of electric energy comes from hydroelectric plants, due to abundant water resources. However, the implementation of these plants causes irreversible impacts on the environment and society. On the other hand, the impacts caused by photovoltaic panels are considerably smaller in the construction phase and zero in the operation phase. This article describes the impacts generated by hydroelectric power plants and gives an overview of the power generated by floating photovoltaic panels using 10% of the area of the reservoirs of the largest hydroelectric power plants in Brazil. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroelectric plant; Photovoltaic power; Floating panel.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200211

Sentinel lymph node detection through radioguided surgery in patients with breast cancer
Provedor de dados: 52
Autores: Abreu,Benedita Andrade Leal de; Santos,Adriana de Morais; Soares,Lívia de Almeida; dos Santos,Antônio Ricardo; Barros,Idna de Carvalho; Abreu,Everardo Leal de; Cruz Filho,Alexandre Jorge Gomes da; Abreu,João Batista de; Vieira,Sabas Carlos.
Biopsy of the sentinel lymphnode (SLNB), the first lymphnode to receive lymphatic drainage from the primary tumor, accurately predicts the axillary lymph node status and, when negative, obviates the need for axillary lymphadenectomy (AL). The aim of this study was, to verify the SLN localization in breast cancer through preoperative lymphoscintigraphy and intraoperative gamma-probe, as well as to demonstrate the benefits of such techniques in preventing complications of AL. Medical records of 228 patients with breast carcinoma, who were underwent SLN localization and, radioguided surgery, from March 2005 to December 2007 were analyzed retrospectively. Data regarding age, tumor characteristic, breast involved, type of surgery, radiopharmaceutical drainage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Sentinel lymph node; Lymphoscintigraphy; Intraoperative probe.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700010

Trends in biological dosimetry: an overview
Provedor de dados: 52
Autores: Amaral,Ademir.
Biological dosimetry (biodosimetry) is based on investigations of induced biological effects (biomarkers) in order to correlate them with radiation dose. Among the indicators employed in biodosimetry, scoring of chromosome aberrations is the most reliable method to quantify individual exposure to ionizing radiation. The technique, applied to circulating lymphocytes, has been developed into a routine procedure to evaluate the dose in the case of real or suspected accidental exposure. Considering the radiosensitivity of lymphocytes in vitro and in vivo as being the same, the dose effect relationship obtained after in vitro irradiation of blood has been widely used, with medico-legal value, for evaluating individual radiation exposure. This report presents an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodosimetry; Physical dosimetry; Biomarkers; Chromosome aberrations.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500017

Antidiabetic Effect of Oleuropein from Olea europaea Leaf against Alloxan Induced Type 1 Diabetic in Rats
Provedor de dados: 52
Autores: Qadir,Nadir Mustafa; Ali,Khabat Anwar; Qader,Suhailah Wasman.
This study have been designed to study the effect of extracted pure oleuropein from Oleaeuropaealeaf against alloxan induced type 1 diabetic rats.Diabetic male ratwas induced by injectingsingle subcutaneous injection of 100 mg/kg b.wof alloxan.Respectively, pure oleuropein compound (5, 10, 15 and 20 mg/kg.b.w)was orally administered once per a day during a period of 40 days ofexperiment.Then, the serum blood was collected for the determination of glucose level,haematological analysis, enzymatic and non-enzymatic antioxidant. Further, pancreatic tissue was evaluated for histological examination. Oleuropein showed a significant rolein attenuating the blood glucose levels and elevation of in-vivo antioxidantafter treating diabetic rats with 5 and 10 mg/kg....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oleuropein; Type 1 Diabetic; Alloxan; Antioxidant; Blood glucose and HBA1C.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100322

The combination of Passiflora alata and Valeriana officinalis on memory tasks in mice: comparison with diazepam
Provedor de dados: 52
Autores: Slomp Junior,Helvo; Seniski,Gerusa; Cunha,Cláudio da; Audi,Elizabeth Aparecida; Andreatini,Roberto.
The effect of a compound combining Valeriana officinalis and Passiflora alata extracts was tested on two mouse memory models: habituation and step-through inhibitory avoidance. Diazepam (1.0 and 2.5 mg/kg) was used as a positive control. Acute diazepam (2.5 mg/kg) before training impaired the habituation and performance in the inhibitory avoidance. On the other hand, acute phytotherapeutic compound (40-160 mg/kg), also before the training session, did not alter mouse behavior in these models. Repeated (15 days) treatment with the compound also did not impair the habituation. At the doses used, no locomotor effect was found. Taken together, the results suggest that, contrary to diazepam, the anxiolytic Valeriana officinalis and Passiflora alata compound did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amnesic; Benzodiazepine; Memory; Mice; Phytotherapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600010

Effect of wearing a swimsuit on hydrodynamic drag of swimmer
Provedor de dados: 52
Autores: Marinho,Daniel Almeida; Mantha,Vishveshwar Rajendra; Vilas-Boas,João Paulo; Ramos,Rui Jorge; Machado,Leandro; Rouboa,Abel Ilah; Silva,António José.
The purpose of this study was to analyse the effect of wearing a swimsuit on swimmer's passive drag. A computational fluid dynamics analysis was carried out to determine the hydrodynamic drag of a female swimmer's model (i) wearing a standard swimsuit; (ii) wearing a last generation swimsuit and; (iii) with no swimsuit, wearing light underwear. The three-dimensional surface geometry of a female swimmer's model with different swimsuit/underwear was acquired through standard commercial laser scanner. Passive drag force and drag coefficient were computed with the swimmer in a prone position. Higher hydrodynamic drag values were determined when the swimmer was with no swimsuit in comparison with the situation when the swimmer was wearing a swimsuit. The last...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human body; Numerical simulations; Computational Fluid Dynamics; Forces; Female.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600007

Phytofabrication of silver nanoparticles using Myriostachya wightiana as a novel bioresource, and evaluation of their biological activities
Provedor de dados: 52
Autores: Vadlapudi,Varahalarao; Amanchy,Ramars.
ABSTRACT  Nanobiotechnology deals with the properties of nanomaterials and their potential uses. Here we report for the first time novel, cost-effective and eco-friendly method for the rapid green synthesis of silver nanoparticles (AgNPs) using leaf extracts of Myriostachya wightiana. The growth of silver nanoparticles was monitored by UV-vis spectroscopy complemented by Zeta potential, dynamic light scattering technique (DLS), Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscope (TEM) and X-ray diffraction (XRD). The surface plasmon resonance (SPR) band found at 434 nm confirmed the reduction of AgNO3 to AgNPs. TEM micrographs revealed that AgNPs are irregular in shape with the size range from 15-65 nm. The functional groups...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanobiotechnology; Myriostachya wightiana; Tribolium castaneum; Xanthomonas campestris.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100417

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan
Provedor de dados: 52
Autores: Pandir,Dilek; Unal,Betul; Bas,Hatice.
Furan is a food and environmental contaminant and a potent carcinogen in animals. Lycopene is one dietary carotenoid found in fruits such as tomato, watermelon and grapefruit. The present study was designed to explore the protective effect of lycopene against furan-induced oxidative damage in streptozotocin (STZ)-induced diabetic rat kidney. At the end of the experimental period (28 days), we found that lycopene markedly decreased the malondialdehide (MDA) levels in the kidney, urea, uric acid and creatinine levels in the serum of furan-treated rats. The increase of histopathology in the kidney of furan-treated rats were effectively suppressed by lycopene. Furthermore, lycopene markedly restored superoxide dismutase (SOD), catalase (CAT), glutathione...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Furan; Lycopene; Kidney; Oxidative stress; Diabetes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100314

A laboratory method to estimate the efficiency of plant extract to neutralize soil acidity
Provedor de dados: 52
Autores: Cassiolato,Marcelo E.; Miyazawa,Mário; Meda,Anderson R.; Pavan,Marcos A.
Water-soluble plant organic compounds have been proposed to be efficient in alleviating soil acidity. Laboratory methods were evaluated to estimate the efficiency of plant extracts to neutralize soil acidity. Plant samples were dried at 65ºC for 48 h and ground to pass 1 mm sieve. Plant extraction procedure was: transfer 3.0 g of plant sample to a becker, add 150 ml of deionized water, shake for 8 h at 175 rpm and filter. Three laboratory methods were evaluated: sigma (Ca+Mg+K) of the plant extracts; electrical conductivity of the plant extracts and titration of plant extracts with NaOH solution between pH 3 to 7. These methods were compared with the effect of the plant extracts on acid soil chemistry. All laboratory methods were related with soil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant residue; Organic acid; Plant alkaline index.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200009

Cell density and solvent are critical parameters affecting formazan evaluation in MTT assay
Provedor de dados: 52
Autores: Gasque,Kellen Cristina da Silva; Al-Ahj,Luana Polioni; Oliveira,Rodrigo Cardoso; Magalhães,Ana Carolina.
The aim of this study was to establish the more accurate protocol for fibroblast cell viability using MTT assay. NIH/3T3 fibroblasts were seeded at the following cell densities: 3.125x10³; 1.156x10(4); 3.125x10(4); 1.156x10(5) and 3.125x10(5) cells/cm². Following 24h of seeding, MTT was added to the wells. After 4h of the MTT addition, different solvents were added to solubilize the formazan crystals: 1) HCl/SDS group- 20% SDS and 0.01 M HCl; 2) EtOH/ HAc group-50% ethanol and 1% acetic acid; 3) DMSO group- 99.5% dimethyl sulfoxide; and 4) PropOH group- 99.5% isopropanol. The absorbance values were measured using a spectrophotometer at 570 nm. The data were analyzed by 2-way ANOVA (p&lt;0.05) and showed that the absorbance average varied according to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell viability; MTT assay; Fibroblast NIH/3T3; Solvents.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300011

Biological response of spontaneously hypertensive rats to the streptozotocin administration
Provedor de dados: 52
Autores: Costa,Virginia Alice Vieira da; Vianna,Lucia Marques.
The sensitivity of adult spontaneously hypertensive rats (SHR) to the diabetogenic effect of streptozotocin (STZ) was studied. The animals were subdivided into three groups: control (citrate buffer), streptozotocin 40 mg/kg or 50 mg/kg, and general biologic parameters were analyzed, in addition to systolic blood pressure, blood glucose and insulin levels determinations. Both doses were able to induce hyperglycemia above 300 mg/dl; however, 50 mg/kg provoked a more pronounced physiological alterations in body weight, diuresis, water and food intake. There was no change on systolic blood pressure with either dose. Results suggested that SHRs did not need doses of streptozotocin above 40mg/kg in order to produce diabetes probably because this strain was much...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptozotocin; SHR; Diabetes; Blood pressure; Insulin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100006

Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta
Provedor de dados: 52
Autores: Ibrahim,Zaharah; Ahmad,Wan Azlina; Baba,Abu Bakar.
A silver uptake study by Pseudomonas diminuta was carried out by growing the bacteria in a chloride-free medium (CFM) containing silver ions (50 muM) in a batch culture. From the results, it was found that higher amounts of silver were accumulated inside the cell during early exponential phase compared to the amount bound at the cell surface. This suggested a possible mechanism for metal uptake during bacterial growth. In view of this, attempts were made to isolate proteins which might be associated with silver-binding properties from cultures of P.diminuta grown in the presence and absence of silver. The proteins were first extracted from the bacterial cultures by precipitation with ammonium sulfate followed by purification using isoelectric focussing and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioaccumulation; Metal-binding proteins; Pseudomonas diminuta.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300001

Value addition of corn husks through enzymatic production of xylooligosaccharides
Provedor de dados: 52
Autores: Samanta,Ashis Kumar; Kolte,A.P.; Elangovan,A.V.; Dhali,A.; Senani,S.; Sridhar,M.; Suresh,K.P.; Jayapal,N.; Jayaram,C.; Roy,Sohini.
ABSTRACT Corn husks are the major wastes of corn industries with meagre economic significance. The present study was planned for value addition of corn husk through extraction of xylan, followed by its enzymatic hydrolysis into xylooligosaccharides, a pentose based prebiotic. Compositional analysis of corn husks revealed neutral detergent fibre 68.87%, acid detergent fibre 31.48%, hemicelluloses 37.39%, cellulose 29.07% and crude protein 2.68%. Irrespective of the extraction conditions, sodium hydroxide was found to be more effective in maximizing the yield of xylan from corn husks than potassium hydroxide (84% vs. 66%). Application of xylanase over the xylan of corn husks resulted into production of xylooligosaccharides with different degree of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn waste; Xylan extraction; Prebiotic; Enzymatic process; XOS.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100512

Effect of paclitaxel (Taxol®) on the biodistribution of sodium pertechnetate (Na99mTcO4) in female Wistar rats
Provedor de dados: 52
Autores: Holanda,Cecília Maria de Carvalho Xavier; Oliveira,Elias Herculano de; Rocha,Louisianny Guerra da; Barbosa,Vanessa Santos de Arruda; Spyrides,Maria Helena Constantino; Aragão,Cícero Flávio Soares; Medeiros,Aldo da Cunha.
The evidence that natural or synthetic drugs can affect the biodistribution of radiopharmaceuticals (radiobiocomplexes) in setting of nuclear medicine clinic is already known. We studied the effect of Paclitaxel, an anti-neoplastic agent for the treatment of solid tumors, on the biodistribution of Na99mTcO4 in female rats. Paclitaxel (1mg/mL/week) was administered into animals in single dose during 3 weeks, with interval of 1 week among them. The control group received NaCl 0.9% solutions by the same via. One hour after the last dose, it was injected Na99mTcO4 in the animals. The percentage of activity per gram (%ATI/g) and biochemical and hematological determinations were performed. A significant increase were found in alanine aminotransferase, aspartate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paclitaxel; Drug interaction; Radiopharmaceuticals; Na99mTcO4; Antitumoral drug; Cancer.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700031

Colon cancer and swimming exercise: effect on wistar rat testes
Provedor de dados: 52
Autores: Freitas,Karine Moura de; Natali,Antônio José; Lunz,Wellington; Costa,Kyvia Lugate Cardoso; Leite,Rodrigo Paula; Dolder,Heidi; Matta,Sérgio Luis Pinto da.
This study was undertaken to determine whether colon cancer (CC) and chronic swimming exercise alter rat testis. Eleven weeks old rats were distributed into control group (n=6) and the groups that were induced to develop CC by dimethylhydrazine injections (nEG, EG0, EG2 and EG4; n=10 each group). In the group nEG, the rats did not swim, whereas groups EG0, EG2 and EG4, underwent a swimming program with distinct loads (0, 2 and 4% of body mass, respectively) for 35 weeks. The morphometry, stereology and cell counts showed damage caused by the CC on the germ epithelium. These results were noteworthy since this was the first report to associate the CC with testicular damage. Swimming exercise had no significant role in reducing, or increasing the CC effects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1; 2-dimethylhydrazine fertility reproduction spermatogenesis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400005

Somatic embryogenesis from immature cotyledons of soybean (Glycine max (L.) Merr.): ontogeny of somatic embryos
Provedor de dados: 52
Autores: Santos,Karla Galvão Bezerra dos; Mariath,Jorge Ernesto de Araújo; Moço,Maria Cecília C.; Bodanese-Zanettini,Maria Helena.
Soybean somatic embryos obtained from immature zygotic embryos were histologically analyzed in order to determine possible ontogenetic routes followed by these embryos. The proembryo stage and globular, heart-shaped, torpedo and cotyledonary embryo stages were found, closely resembling the ontogeny of zygotic embryos. However, the absence of a characteristic suspensor as well as the delay in the establishment of inner organization were the main differences between these two embryogenic processes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Ontogeny; Somatic embryo.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100006

Cloning and characterization of the iron uptake gene iutA from avian Escherichia coli
Provedor de dados: 52
Autores: Tokano,Dorismey Vieira; Kawaichi,Marisa Emiko; Venâncio,Emerson José; Vidotto,Marilda Carlos.
The aim of this work was to isolate, clone and characterize the iron uptake gene iutA from avian pathogenic E. coli (APEC). The iutA gene was isolated from the strain APEC 9, serotype O2:H9, which was cloned in the expression vector pET101/D-TOPO. The gene of 2.2 Kb was sequenced (AY602767, which showed high similarity to the iutA gene from three plasmids, two from APEC, pAPEC-02-ColV (AY545598.4) and pTJ100 (AY553855.1), and one from a human invasive E. coli strain, the pColV K30. The recombinant protein IutA was over expressed in E. coli BL21(DE-3) and was solubilized with urea and purified by Ni-NTA column. This method produced a relatively high yield of r-IutA of approximately 74kDa, which was used to produce the antibody anti-IutA. This anti-IutA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian Escherichia coli; Iron uptake; Aerobactin receptor; Virulence factor; IutA gene.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300006

Properties of films obtained from biopolymers of different origins for skin lesions therapy
Provedor de dados: 52
Autores: Bellini,Márcia Zilioli; Oliva-Neto,Pedro de; Moraes,Ângela Maria.
In this study, the effects of the origin of xanthan used, in combination with chitosan, to prepare films for the treatment of skin lesions were evaluated. The characteristics of the films obtained with xanthan commercially available for the food industry sector and xanthan originated from a fermentation process conducted in a pilot plant were compared. Results showed that the source did not strongly interfere in many of the properties of the films, such as the mechanical properties, cytotoxicity to L929 cells, absorption of simulated body fluid and culture medium, stability in water and saline solution. Hence, even though the properties of biopolymers of different sources might vary, the films prepared with two distinct types of xanthan gum could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterials; Biopolymers; Films; Membranes; Properties and characterization; Biomedical applications.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200289

Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues
Provedor de dados: 52
Autores: Bajaj,Bijender Kumar; Singh,Satbir; Khullar,Mowkshi; Singh,Kaurab; Bhardwaj,Shikha.
The aim of this work was to study the production of fibrinolytic protease by Bacillus subtilis I-2 on agricultural residues. Molasses substantially enhanced (63%) protease production (652.32 U/mL) than control (398.64 U/mL). Soybean meal supported maximum protease production (797.28 U/mL), followed by malt extract (770.1 U/mL), cotton cake (761.04 U/mL), gelatin (742.92 U/mL) and beef extract (724.8 U/mL). Based on the Plackett-Burman designed experiments, incubation time, soybean meal, mustard cake and molasses were identified as the significant fermentation parameters. Ammonium sulfate precipitation and DEAE sephadex chromatography resulted 4.8-fold purification of protease. Zymography showed the presence of three iso-forms in the partially purified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrinolytic Protease; Production; Agricultural residues; Bacillus subtilis I-2.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500653

Antithrombotic Activity of Milk Protein Hydrolysates by Lactic Acid Bacteria Isolated from Commercial Fermented Milks
Provedor de dados: 52
Autores: Pérez-Escalante,Emmanuel; Jaimez-Ordaz,Judith; Castañeda-Ovando,Araceli; Contreras-López,Elizabeth; Añorve-Morga,Javier; González-Olivares,Luis Guillermo.
ABSTRACT In the last decade, thrombosis has been one of the pathologies with high incidence creating great concern in Health Institutes all around the world. Considering this, the aim of this research was to determine the antithrombotic activity of peptides released during lactic fermentation. Reconstituted skim milk powder was fermented by Lactobacillus casei Shirota and Lactobacillus johnsonii LA1 isolated from commercial fermented milks. The hydrolysis degree and proteolytic profile were analyzed by trinitrobenzenesulfonic acid spectrophotometry method and by peptide polyacrylamide electrophoresis gel separation. The milk fermented by Lactobacillus casei Shirota exhibited a higher concentration of free amino groups than that fermented by Lactobacillus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antithrombotic activity; Peptides; Lactic fermentation; Probiotic bacteria; Commercial fermented milks.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100506

Production and characterization of amylases from Zea mays malt
Provedor de dados: 52
Autores: Biazus,Joana Paula Menezes; Souza,Roberto Rodrigues de; Márquez,Jesus Espinoza; Franco,Telma Teixeira; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In this work the α and β-amylase enzymes were obtained from maize (Zea mays) malt and were biochemistry characterized. A germination study to obtain the maize malt with good amylase activity was made. The maize seeds were germinated in laboratory and the enzymatic activity was measured daily. Activity dependence to germination time were fitted to an exponential model (A=A0eµt), which showed that the behaviour of enzymatic activity in the germination process was similar to the growth of the microorganism. Its model could be applied to describe the mechanism of α-amylase production for each maize varieties and others cereals. Maize malt characterization showed that α and β-amylase had optimal pH between 4-6.5, optimal temperature 50 and 90ºC, and molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize seed; Malting; Α and β-amylase; Biochemistry characterization; Germination model.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400024

Consumption of ferulic and p-hydroxybenzoic acids by soybean root tips
Provedor de dados: 52
Autores: Silva,Hélio Cândido da; Herrig,Vanessa; Ferrarese,Maria de Lourdes L.; Ferrarese-Filho,Osvaldo.
Depletion method was used to describe how soybean root consumed ferulic and p-hydroxybenzoic acids in nutrient culture. The results showed that excised cultured soybean roots consumed these allelochemicals very rapidly. At concentrations between 0.1 and 1.0 mM, roots consumed 50% or more of ferulic acid after 6-h depletion. Although consumption started only after 3 h, similar behavior was verified with p-hydroxybenzoic acid.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max L. Merr.; Root; Absorption; Allelochemicals.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300006

Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
Provedor de dados: 52
Autores: Moreira,Keila Aparecida; Diogo,Margarida; Prazeres,Duarte Miguel; Lima Filho,José Luiz de; Porto,Ana Lúcia Figueiredo.
This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene therapy; Hydrophobic interaction chromatography; Plasmid; Purification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014

Computation of Various Domination Numbers of Rolf Nevanlinna (RNP) Collaboration Graph
Provedor de dados: 52
Autores: V,Yegnanarayanan; B,Logeshwary.
ABSTRACT In this paper, we compute various Domination numbers like Outer Connected Domination (OCD), Doubly Connected Domination (DCD), Fair Domination (FD), Independence Domination (ID), 2-Packing (2-P) for Rolf Nevanlinna Prize Winners's Collaboration Graph (RNPCG).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Outer Connected Domination number; Doubly Connected Domination Number; Fair Domination Number; Independence Domination Number; 2-Packing Number; Rolf Nevanlinna collaboration Graph.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100606

Evaluation of hydrocyclone as pre-filter in irrigation system
Provedor de dados: 52
Autores: Soccol,Olívio José; Rodrigues,Lineu Neiva; Botrel,Tarlei Arriel; Ullmann,Mario Nestor.
The objective of this work was to evaluate the efficiency of a hydrocyclone to separate sand in irrigation water. To do this, it an experiment was conducted where the hydrocyclone was operated with pressures and discharge that varied from 10 to 60 kPa and from 1,159.90 L h-1 to 2,603.60 L h-1, respectively. During the tests, the sand concentration in suspension varied from 2.81 g L-1 to 7.01 g L-1. The results showed that the best efficiency was obtained with pressure differentials of 10 and 30 kPa, with cut size (d70) of 50 µm.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water quality; Pre-filters; Efficiency.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200003

Fed-batch decolorization of Poly R-478 by Trametes versicolor
Provedor de dados: 52
Autores: Moreira,María Teresa; Viacava,César; Vidal,Gladys.
Physiological aspects were evaluated to determine optimal conditions for the decolorization of a synthetic dye, Poly R-478, by white-rot fungus Trametes versicolor # 52J. The decolorization experiments were carried out in semicontinuous operation during three cycles to improve the process efficiency. The best decolorization efficiencies (65% to 80%) were obtained in fungal cultures performed in nitrogen limited conditions under aerobic conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decolorization; Trametes versicolor; Poly R-478; Ligninolytic enzymes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200003

Dynamics of allochthonous organic matter in a tropical Brazilian headstream
Provedor de dados: 52
Autores: Gonçalves Júnior,José Francisco; França,Juliana Silva; Callisto,Marcos.
The species composition of the riparian vegetation and the seasonal contribution of input and storage of fine and coarse particulate organic matter were assessed in a 3rd order stretch. Fourteen tree species in the riparian zone were identified, with 3 species contributing with 68% of total litter input: Miconia chartacea Triana (43%), Miconia cyathanthera Triana (16%) and Erythroxylum pelletarianum St. Hil (9%). The allochthonous input of coarse particulate organic matter (CPOM) was composed mainly by leaves (over 50%). Species composition and the contribution of each plant species biomass for vertical, lateral and soil inputs and benthic stocks varied along the study period. The maximum values found in September, November and December coincided with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Riparian vegetation; Input; Standing stock; Cerrado; CPOM.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700014

Introduced and cryptogenic species and their management in Paranaguá Bay, Brazil
Provedor de dados: 52
Autores: Neves,Carolina Somaio; Rocha,Rosana Moreira da.
The aim of this work was to identify the introduced and cryptogenic species in encrusting and associated communities of hard substrates in Paranaguá Bay, Brazil, and to attempt to determine which of these species could negatively affect the local community to suggest management strategies for these species. At least four introduced species were found - a large number in comparison with other port surveys. These were the hydrozoan Garveia franciscana Torrey, 1902, the polychaete Polydora cornuta Bosc, 1902, the barnacles Amphibalanus reticulatus (Utinoni, 1967) and Striatobalanus amaryllis Darwin, 1854, all with potentially harmful impacts. Of the 33 cryptogenic species, four were also listed in the literature as causing negative effects. We propose the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinvasion; Introduced species; Management; Biofouling; Artificial substrates; Paranaguá Bay.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300025

Case-Based Reasoning for the Design of Start-Stop Logic of Hydroelectric Power Stations
Provedor de dados: 52
Autores: Mancini,Fabrízio Nicolai; Aoki,Alexandre Rasi.
ABSTRACT The present article focuses on the construction of an Intelligent System for the Project Start-Stop Logic of Hydropower Plants, work carried out as master dissertation, allowing the retention of knowledge of previous cases which set a valuable intellectual capital, organizing knowledge generated by specialists and consultants of the area. The tool aims at saving time as well as standardizing solutions, allowing future developments for the construction of other necessary logic (such as those related to asset management and tools to aid the operation and maintenance of hydroelectric power plants). The MyCBR, software that proved to be of easy programming, but with some limitations, is used for such an attempt of a shell of current use. The recovery...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydropower plant; Start and stop logic; Intelligent Systems - Case Based Reasoning; MyCBR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200235

Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
Provedor de dados: 52
Autores: Manera,Ana Paula; Kamimura,Eliana Setsuko; Brites,Luciana Machado; Kalil,Susana Juliano.
Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyloglucosidase; Adsorption isotherm; Kinetics adsorption; Ion exchange resin; Purification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019

Effect of nitrogen limitation on cell growth, lipid accumulation and gene expression in Chlorella sorokiniana
Provedor de dados: 52
Autores: Li,Ying Xia; Zhao,Feng Juan; Yu,Dong Dong.
The influence of nitrogen (N) limitation on the cell growth, chlorophyll content, intracellular lipid production and expression levels of two pathway genes in Chlorella sorokiniana was investigated in mixotrophic culture in this study. The maximum biomass concentration of C. sorokinianacultured in modified BG11 medium containing 3, 6, 9, 12 and 15 mM N were 1.60, 2.21, 2.74, 3.18 and 3.21 g/L of dry cell weight, respectively with maximum specific growth rate of 0.180, 0.198, 0.201, 0.203, 0.206 day−1 during culture with an initial N feed of 3, 6, 9, 12 and 15 mM in the first eight days, respectively. The maximum lipid content was 51 % with 3 mM N. However, the maximum lipid productivity of 0.00884 g L−1 day−1 was achieved with 6 mM N. Expression levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrogen limitation; Chlorella sorokiniana; Lipid; Real- time PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300462

Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
Provedor de dados: 52
Autores: Parizzi,Silvânia Quintão Fontes; Andrade,Nélio José de; Silva,Cleuber Antonio de Sá; Soares,Nilda de Fátima Ferreira; Silva,Eldo Antônio Monteiro da.
The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p&lt;0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial adherence; Listeria innocua and Staphylococcus aureus; Stainless steel; Polycarbonate and polypropylene.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011

Moxidectin interference on motor activity of rats
Provedor de dados: 52
Autores: Rodrigues-Alves,Patrícia de Sá e Benevides; Flório,Jorge Camilo; Lebrun,Ivo; Bernardi,Maria Martha; Spinosa,Helenice de Souza.
The present study investigated the effects of t moxidectin (MXD) in some parameters of rat motor function and neurochemical. The general activity in the open field and the motor coordination in the wooden beam were employed to evaluate the MXD effects. The results showed that, in the open field, even at high doses (2.0 and 20.0 mg/kg), the MXD did not alter the locomotion and the rearing frequencies. However, MXD was able to impair the motor coordination of the animals at wooden beam. Neurochemical studies of striatal GABA and dopamine neurotransmitters showed a reduced levels of dopamine and its metabolite, homovanillic acid, without interference on striatal GABA levels. Since GABAergic receptor stimulation had an inhibitory effect on dopaminergic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moxidectin; GABA; Motor coordination; Rat; Open field; Wooden beam.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400012

Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli grown on cane molasses fortified with ethanol
Provedor de dados: 52
Autores: Gomaa,Eman Zakaria.
The aim of this work was to study the production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli isolated from the industrial contaminated soil samples using cane molasses as an inexpensive substrate. The amount of PHA accumulated followed a similar pattern to its growth for each of treatment indicating a growth-related production, yielding maximum PHA production of 54.1 and 47.16% for B. subtilis and E. coli, respectively after 96 h cultivation in the medium containing 6 and 8 % molasses, respectively and decreased thereafter. The growth and PHA yields were improved by introducing 1% ethanol into the molasses medium. Ammonium sulphate and ammonium nitrate at a concentration of 1 g/L served as the best nitrogen sources for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxyalkanoates; Cane molasses; Infrared spectroscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100020

Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph
Provedor de dados: 52
Autores: Raffoul,Tássia; Swiech,Kamilla; Arantes,Mabel Karina; Sousa,Álvaro Paiva Braga de; Mendonça,Ronaldo Zucatelli; Pereira,Carlos Augusto; Suazo,Cláudio Alberto Torres.
The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal cell; CHO-K1; Microcarrier; Growth; Attachment; Hemolymph.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400011

Management of the environmental restoration of degraded areas
Provedor de dados: 52
Autores: Araujo,Izabel Cristina Leinig; Dziedzic,Maurício; Maranho,Leila Teresinha.
The aim of this work was to study ecotechnology for the management of degraded areas originally covered by the Atlantic Rainforest and located at the coordinates 25º31'50''S, 9º09'30''W. The area included 12 islands, each consisting of six jute bags with 20 kg of substrate (cattle manure and soil transposed from forest fragments). In six of these bags, native plants and seeds were also included. Six additional islands were selected randomly in the vicinity as the control. The process of evaluation was monitored through the chemical and granulometric soil analysis and surveys of survival, biometrics, floristic and phytosociological vegetation. An improvement in soil properties was observed where the model was implemented, which could be attributed to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restoration ecology; Environmental degradation; Soil management; Revegetation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200018

Area Triangulation Method for Automatic Detection of Venous Emptying Maneuvers
Provedor de dados: 52
Autores: Sánchez-Gendriz,Ignacio; Hernández-Seoane,D.; Sóñora-Mengana,A.; Pascau-Simón,A.
Abstract Venous refilling time (VRT) can diagnose the presence of venous diseases in lower limbs. In order to calculate VRT it is necessary to determine the End of the Emptying Maneuvers (EEM). First Derivative Method (FDM) can be employed for automatic detection of the EEM, but its sensitivity to artifacts and noise can degrade its performance. In contrast, studies report that Area Triangulation Method (ATM) evinces effectiveness in biosignals point finding. This work compares the exactness of ATM and FDM for recognition of the EEM. The annotations made by 3 trained human observers on 37 photoplethysmography records were used as a reference. Bland-Altman graphics supported the analysis of agreement among human observers and methods, which was complemented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Area Triangulation Method; First Derivative Method; Photoplethysmographic; Venous Refilling Time.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100300

Nictemeral variations for pacu Caranha, Piaractus mesopotamicus (Holmberg, 1887) culture in single - phase
Provedor de dados: 52
Autores: Barreto,Luiz Eduardo Guimarães de Sá; Igarashi,Marco Antonio; Hayashi,Carmino.
Studies related to nictemeral variation were executed in a fish growing pond, during all the seasons of the year, collecting data for dissolved oxygen; temperature; pH and electrical conductivity at the feeding channel on pond's surface, bottom and at streamlet bed. The results showed that Autumn was the season which had the lowest values of water temperature, the interval points, during Spring time, had superior average values of temperature when compared to the ones that were observed at external points. The lowest and highest index of pH were during Autumn season. The electrical conductivity didn't show significant differences during Autumn season. The samples obtained from the streamlet had very high significant differences with the samples of fish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Grow-out; Rearing; Environment.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400017

Reproductive studies in ipecac (Psychotria ipecacuanha (Brot.) stockes; Rubiaceae): pollen development and morphology
Provedor de dados: 52
Autores: Souza,Margarete Magalhães; Martins,Ernane Ronie; Pereira,Telma Nair Santana; Oliveira,Luiz Orlando de.
The aim of this work was to carry out the reproductive studies on Brazilian accessions of ipecac, Psychotria ipecacuanha. It presented heterostyly, with brevistylous and longistylous flowers. The pollen development was observed from the sections of the anthers embedded in resin. Anther development was normal as usually observed in dicotyledones, displaying four layers: outer epidermis, endothecium, middle layer and inner tapetum. The pollen was bicellular and filled with starch at the microspore stage. Pollen morphology was studied using SEM, which showed pollen polymorphism within and between the two floral morphs. Five types of pollen with reticulate or perforate exine were identified. The characteristics showed that the sexual process was as important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipecac; Heterostyly; Anther anatomy; Microsporogenesis; Exine.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500015

Relationship Between Parameters of Development and Functional Compounds of Yacon Leaves
Provedor de dados: 52
Autores: Ferreira,Bruna Mayara Roldão; Dagostin,João Luiz Andreotti; Andrade,Eriel Forville de; Takashina,Thiago Atsushi; Ellendersen,Luciana de Souza Neves; Masson,Maria Lucia.
Abstract Yacon is a tuberous root from the Andean region being increasingly grown across the world due to the low caloric values of their roots and the functional properties of extractions of its leaves. However, there is still a gap in the knowledge of how the plant develops, and if physical and chemical changes are noticed regarding maturity. The subject of this work was to investigate the dynamics of a group of parameters and to establish correlations across them. To achieve this goal, leaves collected from three different parts of the plant in three months were assessed regarding size (plant height and leaf area), color and the presence of sesquiterpene lactones. Different methods of extraction were also studied and the total phenolic content was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smallanthus sonchifolius; Sesquiterpene lactones; Physical parameters; Chemical parameters; Phenolic content.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100430

Growth evaluation of Oreochromis niloticus (Cichlidae, Neopterygii) exposed to Trichlorfon
Provedor de dados: 52
Autores: Guimarães,Ana Tereza Bittencourt; Calil,Patrícia.
The aim of this work was to evaluate the influence of Trichlorfon on the growth of cultured fishes. Using Oreochromis niloticus as fish model, bioassays were performed in the laboratory and field. Biometry was performed for 16 weeks. The treatment started after the sixth week of biometry, with the application of five weekly doses of 0.25 ppm of Trichlorfon. Growth data were compared by longitudinal analyses. In the laboratory, the treated fishes showed a reduction of growth; however, the statistical significance was only seen in the variable weight. In the field, the growth of treated fish was higher than that of the controls. This suggested that the treatment should be done in tanks with lower relative biomass to maintain the good zootechnic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organophosphate Tilapia; And aquaculture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200012

Acute intraperitoneal mercury chloride contamination and distribution in liver, muscle and gill of a neotropical fish Hoplias malabaricus (BLOCK, 1794)
Provedor de dados: 52
Autores: Jesus,Taise Bomfim de; Almeida,Priscila Gontijo Aguiar de; Vergílio,Cristiane dos Santos; Machado,André Luiz dos Santos; Carvalho,Carlos Eduardo Veiga de.
The present study investigated with the distribution of mercury chloride in muscle, liver and gills of Hoplias malabaricus contaminated through intraperitoneal injection (6 µg in 0.1mL of PBS) for a period of 24, 48, 72 and 96h. The liver, gill and muscle were analyzed for mercury content in an ICP/AES (Varian Liberty II) with vapor generating accessory (VGA 77). The muscle and liver tissues presented the same contamination pattern increasing concentrations in 24 h of exposure with a decrease in Hg concentration with 72 h and a new increase in Hg concentrations with 96 h of exposure. The Hg concentrations in contaminated organisms were always higher than the control although only for liver samples the difference was statistically significant. Liver samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inorganic mercury; Liver; Acute effects; Intraperitoneal contamination.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200021

Microbial activity in soil cultivated with different summer legumes in coffee crop
Provedor de dados: 52
Autores: Balota,Elcio Liborio; Chaves,Julio César Dias.
A field experiment was conducted for ten years in a sandy soil in the north part of the Paraná State, Brazil. The soil samples were collected at 0-10 cm depth, both under the coffee canopy and in the inter row space between the coffee plants, in the following treatments: Control, Leucaena leucocephala, Crotalaria spectabilis, Crotalaria breviflora, Mucuna pruriens, Mucuna deeringiana, Arachis hypogaea and Vigna unguiculata. The legume crops influenced the microbial activity, both under the coffee canopy and in the inter row space. The cultivation of Leucaena leucocephala increased the microbial biomass C, N and P. Although L. leucocephala and Arachis hypogaea provided higher microbial biomass, the qCO2 decreased by up to 50% under the coffee canopy and by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial biomass; Mulching; Green manure; Soil fertility.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100005

In vitro inhibition of Pseudomonas aeruginosa adhesion by Xylitol
Provedor de dados: 52
Autores: Sousa,Letícia Pinheiro de; Silva,Annelisa Farah da; Calil,Natalia Oliveira; Oliveira,Murilo Gomes; Silva,Silvio Silvério da; Raposo,Nádia Rezende Barbosa.
This study evaluated, in vitro, the antimicrobial activity and the anti-adherent property of xylitol (0.5, 2.5 and 5.0%, w/v) on two Pseudomonas aeruginosa strains (ATCC 9027 and clinical). The assay of antimicrobial activity was performed to determine a minimum inhibitory concentration (MIC) and the adhesion test was performed, by which the parameters regarding, growth in the culture medium, number of colony forming units (CFUs) released and slide evaluation by scanning electron microscopy (SEM) were analyzed. The Statistical Package for the Social Sciences (SPSS) was employed for statistical analysis. Results showed that xylitol had no antimicrobial activity on these strains; however, the inhibition of bacterial adherence was observed in microphotographs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial adhesion; Pseudomonas aeruginosa; Prevention and control; Xylitol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500004

Physical attributes of kaolinitic and oxidic oxisols resulting from different usage systems
Provedor de dados: 52
Autores: Centurion,José Frederico; Beutler,Amauri Nelson; Souza,Zigomar Menezes de.
The objective of this study was to assess the physical attributes of a kaolinitic oxisol, medium texture (Haplustox) and an oxidic oxisol, clayey (Eutrustox) under different usage systems, localized in the region of Jaboticabal, SP, Brazil. The usage systems were sugarcane, cotton and forest. Parameters such as soil bulk density, total porosity, macro and microporosity at the depths of 0.0-0.1; 0.1-0.2; 0.2-0.3, and 0.3-0.4 m were evaluated. Haplustox showed greater bulk density and smaller total porosity, macro and microporosity. The usage increased the bulk density in 0.0-0.3 m depth, with greater effects on the kaolinitic oxisol, mainly in 0.1-0.2 m depth in the areas cultivated with sugarcane.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Cotton; Forest; Mineralogy; Porosity; Bulk density.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500007

Novel Food Supplements Formulated With Spirulina To Meet Athletes’ Needs
Provedor de dados: 52
Autores: Carvalho,Lisiane Fernandes de; Moreira,Juliana Botelho; Oliveira,Mariana Souza; Costa,Jorge Alberto Vieira.
ABSTRACT The food, training, and health are crucial for a good performance in sports. Intense physical activity takes the athlete to maintain a very unstable balance between energy demand and consumption of nutrients. Spirulina microalga has a nutritional profile that renders it an ideal food supplement, because has high protein content, also contains vitamins, minerals, and pigments. In this context, the study aimed to develop, characterize and evaluate the stability of foods enhanced with Spirulina, which are intended for athletes. In this study, six different supplements were developed (electrolyte replenisher, muscle enhancer, and recovery supplement), without and with Spirulina. The electrolyte replenisher with Spirulina compared to the product...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Athletes; Microalgal biomass; Performance; Supplement.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100315

Use of Ursodeoxycholic Acid on Post-menopausal Obesity, Hepatic steatosis and Plasma Profile as an Alternative Treatment for Hormone Replacement Therapy
Provedor de dados: 52
Autores: Franzoni,Angelo Christiano; Amorim,Aryane Martins; Silva,João Victor Mariano da; Storti,João Augusto Pires; Oliveira,Monique Cristine de.
ABSTRACT The aim of this study was to evaluate the efficacy of the ursodeoxycholic acid (UDCA) as an alternative treatment for menopause pathologies. For this, female Swiss-CD-1 mice were ovariectomized (OVX); SHAM operated mice served as controls. Ten weeks after operation, each group of mice was treated during four weeks with either UDCA, or 17(-estradiol+progesterone. The blood and tissues (liver and periuterine adipose tissue - PUAT) were harvested. The OVX mice had increase body weight, accompanied by liver lipid accumulation. UDCA and HRT treatments reduced these parameters. PUAT was also increased in OVX group and UDCA and HRT treatments reduced this. Histology confirmed the results in the liver and PUAT. The plasma glucose levels were elevated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver disease; Menopause; Obesity; Ursodeoxycholic acid.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600898

Quantification of iodine in porous hydroxyapatite matrices for application as radioactive sources in brachytherapy
Provedor de dados: 52
Autores: Lacerda,Kássio André; Lameiras,Fernando Soares; Silva,Viviane Viana.
In this study, non-radioactive iodine was incorporated in two types of biodegradable hydroxyapatite-based porous matrices (HA and HACL) through impregnation process from sodium iodine aqueous solutions with varying concentrations (0.5 and 1.0 mol/L) . The results revealed that both systems presented a high capacity of incorporating iodine into their matrices. The quantity of incorporated iodine was measured through Neutron Activation Analysis (NAA). The porous ceramic matrices based on hydroxyapatite demonstrated a great potential for uses in low dose rate (LDR) brachytherapy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxyapatite; Brachytherapy; Neutron activation analysis (NAA).
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400016

Evaluation of the Redox State of Wistar Rats Submitted to High-Fat Diet Supplemented With Infusion of Ilex paraguariensis
Provedor de dados: 52
Autores: Silva,Clédia Flores; Nardin,Patrícia; Grings,Mateus; Uecker,Julia Neitzel; Cerqueira,Jenifer Heller; Rincón,Joao Alvarado; Abib,Renata Torres; Schneider,Augusto; Barros,Carlos Castilho; Andreazza,Robson; Pieniz,Simone.
ABSTRACT This study aimed to evaluate the effect of Ilex paraguariensis infusion on redox state of Wistar rats submitted to high-fat and standard diet. Glutathione determination and lipid peroxidation in the hippocampus tissues and liver was performed, as well as the analysis of gene expression of superoxide dismutase, catalase and glutathione peroxidase by real-time polymerase chain reaction. The results from hippocampus showed that the groups fed with standard diet exhibited significant reduction of lipid peroxidation when supplemented with Ilex paraguariensis. The analysis from glutathione determination in the hippocampus showed a significant increase in glutathione activity in the group treated with high-fat diet and Ilex paraguariensis. In the liver,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Bioactive compounds; Oxidative stress; Antioxidant defense.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100328

Maturation of seeds of Caesalpinia echinata Lam. (Brazilwood), an endangered leguminous tree from the Brazilian Atlantic Forest
Provedor de dados: 52
Autores: Borges,Igor Ferrari; Giudice Neto,João Del; Bilia,Denise Augusta Camargo; Figueiredo-Ribeiro,Rita de Cássia Leone; Barbedo,Claudio José.
The present work describes changes during the maturation process of seeds of Caesalpinia echinata Lam. Individual flowers were tagged in the day of their anthesis and the pods were collected directly from the branches from 32 to 65 days after flowering (DAF). Results obtained suggested that physiological maturity of C. echinata seeds occurred ca. 60-65 DAF, immediately before shedding, when seeds had 30-40% water content.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Harvesting; Pau-brasil pernambuco physiological maturity seed germination.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800001

Reproduction period of Mimagoniates microlepis, from an Atlantic Forest Stream in Southern Brazil
Provedor de dados: 52
Autores: Braga,Marcelo Rennó; Aranha,José Marcelo Rocha; Vitule,Jean Ricardo.
Mimagoniates microlepis was collected between January and December/2002. Three sample points were chosen at higher, medium and lower portions of Ribeirão river, located at the east of Paraná state. The reproduction period was observed by the frequency of gonad developmental stages, variation of the gonosomatic relation (RGS) and gonad condition (K). The average length at the first sexual maturity (L50) and the average length when 100% of individuals were adults (L100) were calculated. The intensity of the reproductive activity (IAR) was estimated at each sample point. The reproduction period started in the winter and ended at the end of spring. The high values of IAR encountered demonstrated that the reproductive activity occurred in the whole study area,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal stream; Blue tetra; East basin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200014

Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants
Provedor de dados: 52
Autores: Coelho,Cileide Maria Medeiros; Bellato,Cláudia Mattos; Garcia,Andréia Karime Marcelino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.
The in vitro synthesis of phytate was studied in common bean fruit explants. Different concentrations of sucrose; phosphorus (P); myo-inositol; abscisic acid (ABA); glutamine and methionine, were tested. Fixed concentrations of these compounds were tested at different periods (0, 3, 6 and 9 days). Variation in phytate coincided with different concentrations of sucrose, myo-inositol, P and ABA for the duration tested. These compounds caused an accumulation of phytate and were more effective in the presence of myo-inositol and P. The accumulation of P varied less than phytate for the different treatments tested in vitro. In conclusion, P, sucrose, ABA, and myo-inositol caused an increase in the phytate of bean seed, showing that it could be possible to alter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abscisic acid; Glutamine; Phosphorus; Phytic acid; Sucrose.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100020

Effect of deoxynivalenol and T-2 toxin in malt amylase activity
Provedor de dados: 52
Autores: Garda-Buffon,Jaqueline; Baraj,Edlira; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the α-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the β-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aminolitic enzymes; Fermentation; Trichothecenes; Barley.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300002

Survival and the growth of pintado (Pseudoplatystoma corruscans) post-larvae on different salinities
Provedor de dados: 52
Autores: Beux,Luis Fernando; Zaniboni Filho,Evoy.
The aim of this study was to evaluate the survival and growth of pintado, Pseudoplatystoma corruscans, post-larvae (PL) submitted to seven salinities: S1: 0; S2: 1.7; S3: 3.3; S4: 5; S5: 6.7; S6: 8.3 and S7: 10‰ distributed in a complete randomized design with three replicates. PL were stocked in 5-l round plastic containers at a density of 15 PL/liter and maintained in the dark. The water was salinized with iodine-free sodium chloride (NaCl). PL were fed six times a day with 500 Artemia sp. nauplii/PL. Survival was high up to salinity 1.7‰, both on the fifth and on the tenth day of experiment, but decreased quickly in salinities above 5‰. Better growth rates were observed in salinities 0 and 1.7‰, however, high growth observed in freshwater was due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudoplatystoma corruscans; Salinity; Pintado; Artemia sp.; Post-larvae; Survival.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500010

Factors associated with the non detection of the sentinel node in breast cancer
Provedor de dados: 52
Autores: Guirao,Sara; Benítez Segura,Ana; Bajén,Maria Tereza; Brulles,Ynonet Ricart; Salvado,Jaume Mora; Vilardell,Ana Domenech; Rodriguez Gassen,Alba; Engronyat,Manel Roca; Puchal Añe,Rafael; Martin-Comin,Josep.
The aim of the work was to analyse the potential influence of different factors on the surgical detection of sentinel lymph node in breast cancer. The procedure has been performed in 704 patients. In 43 out of the 704 cases, the SN was not detected, 24 were palpable and 19 had no palpable tumors. Lymphoscitigraphy was done in 2 h p.i. of 37-55 MBq of 99mTc-nanocolloid. The day before surgery in palpable tumours and 4 h previous to surgery in non-palpable tumours, surgical detection was performed using a gammaprobe. The following factors were analysed: patient age, tumour size, breast quadrant tumour localization, scintigraphic visualizatio,n and the palpation of the tumour. Results: Patient age was significantly (p&lt;0.001) higher in the patients in whom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sentinel node; Breast cancer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700026

Tratamento biológico de efluentes de indústrias de papel após floculação e coagulação com sais de alumínio e polieletrólitos naturais
Provedor de dados: 52
Autores: Reis,Edson M. dos; Nozaki,Jorge.
The flocculation and sedimentation of recalcitrant organic compounds of high molecular weight using natural polyelectrolytes, extracted from the cactus Cereus peruvianus and Opuntia ficus indica, have been studied. Organic compounds of high molecular weight from effluents of paper and pulp industries are difficult for biological degradation. However, using a mixture of aluminum salts and natural polyelectrolytes, the aggregation and settling properties of recalcitrant organics were increased if compared with conventional methods of wastewater treatment using only aluminum or iron sulfate. After flocculation, sedimentation, and filtration, the effluent was submitted to biological degradation by activated sludge process. The main advantages of using a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flocculation; Natural polyelectrolytes; Biological degradation; Activated sludge process; Paper and pulp effluents.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100016

Growth performance, carcass characteristics and meat quality of finishing bulls fed crude glycerin-supplemented diets
Provedor de dados: 52
Autores: Françozo,Mônica Chaves; Prado,Ivanor Nunes do; Cecato,Ulysses; Valero,Maribel Velandia; Zawadzki,Fernando; Ribeiro,Ossival Lolato; Prado,Rodolpho Martin do; Visentainer,Jesuí Vergílio.
This study was conducted to evaluate the performance, carcass characteristics and chemical composition of Longissimusmuscle (LM) of the bulls. Twenty-four Nellore bulls were used in a complete randomised design. The bulls were randomly assigned to one of the three diets containing 0, 5 or 12% glycerin. Final BW and ADG were similar (P&amp;gt;0.05) between the bulls fed with 5 or 12% of glycerin but were higher (P&lt;0.10) compared to the bulls fed without glycerin. DMI and feed efficiency was not affected (P&amp;gt;0.05) by glycerin level. Hot carcass weight increased (P&lt;0.10) when glycerin was fed at 5%. Glycerin level did not affect (P&lt;0.05) the dressing percentage and fat thickness. LM increased (P&lt;0.10) when glycerin was fed at 12%. Dietary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Fat; Glycerin; Human health; Silage corn.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200019

Seasonal variations in blood parameters of the Amazonian manatee, Trichechus inunguis
Provedor de dados: 52
Autores: Colares,Elton P.; Colares,Ioni G.; Bianchini,Adalto; Santos,Euclydes A.
Seasonal variations in body weight, food consumption and blood glucose, total lipids, urea, total proteins, albumin and globulins of captive Amazonian manatees, Trichechus inunguis, were determined. Body weight changed significantly along the year, increasing from autumn to spring and decreasing in summer. The mean daily food intake of paragrass remained almost unchanged along the year. Paragrass administered to the manatees showed important variations in crude protein and lipid content along the year. No significant differences in blood parameters were registered between males and females in all seasons. Further, there were no significant differences in blood total proteins, albumin and globulins along the year. On the other hand, significant differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian manatee; Trichechus; Blood biochemistry; Nutrition; Amazon.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200005

Production of Flammulina velutipes on coffee husk and coffee spent-ground
Provedor de dados: 52
Autores: Leifa,Fan; Pandey,Ashok; Soccol,Carlos R.
Solid state cultivation (SSC) was carried out to evaluate the feasibility of using coffee husk and spent-ground as substrates for the production of edible mushroom Flammulina under different conditions of moisture and spawn rate. The strain of F. velutipes LPB 01 was adapted for a coffee husk extract medium. Best results were obtained with 25% spawn rate, though there was not much difference when lower spawn rates (10-20%) were used. Ideal moisture content for mycelial growth was 60% and 55% for coffee husk and spent-ground, respectively. With coffee husk as substrate, first fructification occurred after 25 days of inoculation and the biological efficiency reached about 56% with two flushes after 40 days. With spent-ground as substrate, first...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flammulina velutipes; Coffee husk; Coffee spent ground; Solid state cultivation; Fructification; Biological efficiency.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200015

Intraerythrocytic organic phosphates and hemoglobins of skua - Catharacta maccormicki (Stercoraridae): at two different stages of the year in relation to Antartic migration
Provedor de dados: 52
Autores: Landini,Gustavo Fraga; Di Vito Neto,Alfredo; Schwantes,Arno Rudi; Schwantes,Maria Luiza Barcellos; Santos,Marcelo dos.
Catharacta maccormicki blood samples were collected in the winter (October) and in the summer (February) in order to study the intraerythrocytic organic phosphates, hemoglobin (Hb) electrophoretic patterns, oxygen blood equilibrium and stripped Hbs, as well as the effect of 2,3-biphosphoglycerate (BPG) and inositol hexaphosphate (IHP) on oxygen affinity. All the samples (five from the winter and five from the summer) showed the same electrophoretic pattern: one minor fast component and one major slow one. No differences in oxygen affinity and Bohr effect in the samples collected in the winter and in the summer were found. Oxygen affinity was higher in the stripped Hb than in the blood. BPG seemed to have no effect on the functional properties of skua Hb...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; Blood; Electrophoresis; Cold; Affinity; Oxygen.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400010

Effectiveness of estradiol valerate on sex reversion in Astyanax altiparanae (Characiformes, Characidae)
Provedor de dados: 52
Autores: Bem,Jaqueline Cristina de; Fontanetti,Carmem Silvia; Senhorini,José Augusto; Parise-Maltempi,Patricia Pasquali.
The objective of this study was to evaluate the effectiveness of estradiol valerate in an attempt to feminize the male Astyanax altiparanae. One thousand newly-hatched larvae were randomly distributed in four incubators. During a period of 30 days, estradiol valerate was administered in different dosages (20, 40, and 80 mg/kg of diet); the control group did not receive hormone in the diet. After 161 days of hatching, biometry was performed on 50 fishes per treatment group, and the gonads were analyzed. The hormone treatments were effective in feminizing A. altiparanae, achieving 70-76% of the desired sex, while the control treatment had 44% females. The hormone did not affect the growth of fish.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax altiparanae; Characidae; Feminization; Sex Reversion; Estrogen.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200015

Population structure and condition factor of Pseudotothyris obtusa (hypoptopomatinae) from three coastal streams in southern Brazil
Provedor de dados: 52
Autores: Takeuti,Denise de Freitas; Verani,José Roberto; Aranha,José Marcelo Rocha; Menezes,Márcia Santos de.
Population structure features and condition factor of Pseudotothyris obtusa were compared between three coastal streams in southern Brazil. Fishes were monthly collected through electric fishing and measured in the total length. Fifteen fishes from each stream were dissected to identify their sex. The structure in size, sex ratio and young/adults ratio of populations were analysed and the length-weight relationship was obtained. The condition factor (K1) and the relative condition factor (Kn) were calculated for each stream. Fishes were grouped in 11 lenght classes of 3mm. The intermediate and bigger size classes were preponderant in the "Mergulhão" and "Colônia Pereira" streams, and the smaller and intermediate ones in the "Ribeirão" stream. Females...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal streams; Pseudotothyris obtusa; Hypoptopomatinae; Population structure; Condition factor.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400004

Fertility Enhancing Potency of Omega-3 Fatty Acids in Male Rats
Provedor de dados: 52
Autores: Nnamonu,Emmanuel Ikechukwu; Mgbenka,Bernard Obialor; Mbegbu,Edmund Chidiebere.
Abstract Cases of some grave side effects of some allopathic medicines used in treatment of infertility has caused a global need for alternatives with minimal or no side effect, hence the demand to evaluate the fertility enhancing potential of omega-3 fatty acids (O3FA) in male rats. This study evaluated the fertility enhancing potential of omega-3 fatty acids (O3FA) in male rats. Seventy-two sexually mature male albino rats 11-13 weeks of age, weighing between 171 - 180 g were assigned into six groups (I - VI) fed graded doses of O3FA. Administration of O3FA lasted twenty-eight days at 48 hour intervals. At the end of the treatment, organosomatic index of testes, testicular and epididymis sperm cells counts and testicular histology were assessed following...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Omega-3 fatty acids; Fertility; Histology; Potency; Male rat.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100318

Thermocyclops decipiens (Kiefer, 1929) (Copepoda, Cyclopoida) as indicator of water quality in the State of Minas Gerais, Brazil
Provedor de dados: 52
Autores: Landa,Giovanni Guimarães; Barbosa,Francisco Antonio Rodrigues; Rietzler,Arnola Cecilia; Maia-Barbosa,Paulina Maria.
The aim of this work was to study the possible utilization of Thermocyclops decipiens as indicator of the trophic degree and water quality of some watersheds of the state of Minas Gerais. From the beginning of the decade of 90, T. decipiens was recorded in several water collections of the state, including the basins of the rivers Paranaíba, São Francisco, Doce and Grande, within environments mainly of meso and eutrophics characteristics. Of the 28 places of occurrence of the species and according to the water quality index (WQI) used by Environmental Foundation of Minas Gerais (FEAM), 7 of these environments presented WQI good, 16 medium, 4 bad and one very bad. This classification corroborated the hypothesis that T. decipiens could be used as indicator...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thermocyclops decipiens; Water quality; Bioindicator.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400015

Erratum: Characteristics and Mechanisms of Biosolubilization of Rock Phosphate by Aspergillus japonicus
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100920

Encapsulation of Red Cabbage (Brassica oleracea L. var.capitata L. f. rubra) Anthocyanins by Spray Drying using Different Encapsulating Agents
Provedor de dados: 52
Autores: Bernstein,Anahi; Noreña,Caciano Pelayo Zapata.
ABSTRACT This study aimed to evaluate the influence of the drying air temperature (140 and 160°C) and the concentration of encapsulating agents gum Arabic and polydextrose (10 and 15%) on the physicochemical characteristics of the red cabbage extract obtained through extraction in acidulated water and drying using a spray dryer. The anthocyanin retention, antioxidant activity, water activity, solubility, color and microstructure of the final product were analyzed. Results showed that increase in the temperature did not cause significant changes in the anthocyanin quantity and antioxidant activity. The microscopic analysis revealed that for both the encapsulating agents, the absence of fissures and porosity in the particle surfaces produced powder with high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spray drying; Red cabbage; Anthocyanins; Gum arabic; Polydextrose.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600944

Protective Effects of Geraniol on Long Term Renal Ischemia/Reperfusion Injury in Rats
Provedor de dados: 52
Autores: Can,Senanur; Canbek,Mediha.
Abstract Possible protective effects of geraniol, known as antioxidant properties, were analyzed biochemically and histologically on experimental long-term renal ischemia/reperfusion I/R injury in rats. This study used 3-4 months old male Wistar albino rats and were divided into 4 groups (n = 7) by random selection: Group I (Sham Group), Group II (I/R+SF), Group III (I/R+50 mg/kg geraniol), and Group IV (I/R+100 mg/kg geraniol). A right nephrectomy was performed in all groups under anesthesia. Groups I and II were inoculated with SF (1 ml/kg) and Groups III and IV were inoculated with 50 mg/kg and 100 mg/kg of geraniol, injected intraperitoneally. For Groups II, III, and IV, I/R durations were determined to be 60 mins ischemia and 24 hours reperfusion. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemia; Reperfusion; Geraniol; Antioxidant; Protective effects; Rat.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100314

Mating strategies and aggressive combat in wingless neotropical fig wasp males
Provedor de dados: 52
Autores: Pereira,Rodrigo Augusto Santinelo; Prado,Angelo Pires do.
The aim of this work was to study the wingless males of pollinating (Pegoscapus tonduzi) and two nonpollinating fig wasp species, genus Idarnes, associated with figs (Ficus citrifolia) in Brazil to answer the following questions: 1) Do wingless males of Idarnes spp. and P. tonduzi show different male-male competition strategies?; and, 2) Do the injury within-species variables correlate with fig/population features? Consistently higher injury levels were observed in the two species of Idarnes than in the pollinator species. The results suggested that aggressive confrontations were involved in the mating strategies of Idarnes, whereas non-aggressive strategies were shown by males of P. tonduzi. Generally, injury variables in Idarnes spp. correlated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaonidae; Contest competition theory; Ficus; Fighting behaviour; Hymenoptera; Moraceae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400013

Purification of metallo-B-lactamase CcrA from Bacteroides fragilis with salting-out method
Provedor de dados: 52
Autores: Liu,Cheng-Cheng; Zhai,Le; Shi,Ying; Yang,Ke-Wu.
A simple and effective salting-out method was developed for the purification of the metallo-β-lactamase CcrA from Bacteroides fragilis based on the plasmid pMSZ02, in which the crude protein secreted into growth medium was precipitated by 80% sulfate saturation of the medium and purified with Q-Sepharose to offer pure CcrA with yield of 20.1 mg per litter medium. The dependence of the amount of protein precipitation on sulfate saturation was investigated, which showed that more than 80% sulfate saturation resulted the maximum protein precipitated. The purified CcrA was evaluated by steady-state kinetics using penicillin G and cephalothin V as substrates, which showed the Km values of 68±2 and 17±2 µM and Kcat values of 63±1 and 102±3 S-1, respectively. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metallo-B-lactamase CcrA; Plateau elution profile; Purification; Salting-out method.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500012

Determination of trace metals in electrolytic copper by ICP OES and ICP-MS
Provedor de dados: 52
Autores: Santos,Éder José dos; Herrmann,Amanda Beatriz; Olkuszewski,José Luiz; Saint'Pierre,Tatiana D.; Curtius,Adilson José.
The performance of axial view ICP OES and ICP-MS techniques were compared through the determination of As, Fe, Mn, Pb, Sb and Sn in electrolytic copper. Samples were prepared by two procedures: 1. Total dissolution with 5 % v/v HNO3 and 2. Dissolution with 1.4 % v/v HNO3 plus 2.0 % v/v H2SO4, followed by separation of the Cu by electrodeposition. The methods were applied for the analysis of standard copper solutions, using calibration against aqueous solutions with or without the addition of Cu. The results obtained by the two techniques were similar, but the separation of Cu from the sample matrix proved to be more adequate for practical reasons.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ICP-MS; Axial view ICP OES; Electrolytic copper; Electrodeposition.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600002

Neuropile organization in the brain of Acromyrmex (Hymenoptera, Formicidae) during the post-embryonic development
Provedor de dados: 52
Autores: Soares,Paula Andréa Oliveira; Delabie,Jacques Hubert Charles; Serrão,José Eduardo.
Neuropile is the region of the central nervous system where the synapses and neurons branching occur. During the development of an holometabolous insect can occurs break of the neurons fibers forming new axon and dendrites and their distribution in brain neuropile is organized so as to reflect specific nervous functions of adult insects. The components of this organization were observed and discussed in this study in the ant Acromyrmex octospinosus, evidencing similar features to those described for A. subterraneus subterraneus, among other insects for the which ones this information is available.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuropile; Nervous system; Ant; Neuronal reorganization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400017

Godoy &amp;amp; Godoy Compression Sleeve in the Treatment of Arm Lymphedema: New Concepts for Materials
Provedor de dados: 52
Autores: Godoy,José Maria Pereira de; Godoy,Ana Carolina Pereira de; Godoy,Maria de Fatima Guerreiro.
ABSTRACT The aim of this work was to study on a new low-elastic textile that fulfilled the criteria of fabrics for the manufacture of compression garments to treat lymphedema. The evolution of Godoy &amp; Godoy compression sleeves was performed by following the patients treated for arm lymphedema. Sixty-six patients with ages ranging from 35 to 83 years and a mean of 58.8 years were included in this study. Diagnosis, by the clinical evaluation confirmed by the volumetry, was defined as a volume difference of more than 200 mL between the arms. All the participants were submitted to treatment sessions once, or two times weekly in an outpatient setting. The material used for the compression sleeve in this study was commercialized in Brazil under the name of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphedema; Compression sleeves; Day-to-day activities.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600864

Optimizing the operational conditions of a membrane bioreactor used for domestic wastewater treatment
Provedor de dados: 52
Autores: Viana,Priscilla Zuconi; Nobrega,Ronaldo; Jordão,Eduardo Pacheco; Azevedo,José Paulo Soares de.
This study evaluated the performance of a sidestream membrane module combined with an aeration system for the treatment of municipal wastewater. To investigate the membrane's behavior and to control fouling, trials in laboratory units were conducted. In these tests, optimal values were established for some operational parameters, such as crossflow velocity, transmembrane pressure and air supply to continuously flush the membrane surface. Air supply improved the behavior of the permeate flux over time. After six hours operation, the stabilized flux was 35 L/m².h at a total pressure of 0.40 bar (wastewater pressure of 0.05 bar and air pressure of 0.35 bar) and Reynolds Number of 4,600. All permeate samples analyzed indicated absence of fecal coliform and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Membrane bioreactor; Reuse; Wastewater treatment; Membranes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400015

Storage of purified biogas (biomethane) at low pressure
Provedor de dados: 52
Autores: Feroldi,Michael; Caroline,Andressa; Borba,Neves, Carlos Eduardo; Arantes,Mabel Karina; José,Alves Helton.
ABSTRACT The global use of fossil energy sources has led to the need to develop technologies capable of mitigating its consequences. One such option is anaerobic biodigestion, which is already scientifically well-established, although the use of its main products (biogas and biomethane) still requires further development before they can be employed in automotive vehicles, largely due to technical issues concerning their storage. The storage of methane using adsorption (Adsorbed Natural Gas - ANG) is a technology that requires less energy and moderate pressures and temperatures. However, the main storage techniques continue to be those that involve high pressures and/or cryogenic temperatures. The present work evaluates the performance of an unprecedented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adsorption; Biogas; Vehicle fuel.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200200

Potentiality of Using Mechanically Separated Meats of Nile Tilapia in Fishburgers: Chemical, Physical and Sensory Characterization
Provedor de dados: 52
Autores: Costa,Denise Pinheiro Soncini da; Gonçalves,Tania Maria Vinturin; Conti-Silva,Ana Carolina.
Abstract Fishburgers were made with washed mechanically separated meat (MSM) from carcass and filleting by-product (both washed and unwashed) of the Nile tilapia. The proximal composition of fishburgers was different and varied according to the raw material used, as well the color parameters. Fishburgers with MSM from filleting by-product had lower hardness and springiness, and fishburger with MSM from unwashed filleting by-product had higher yield and lower reduction in thickness. The fishburgers containing MSM from filleting by-product had better sensory acceptance, due to higher color intensities (sensory), fish odor, tenderness, succulence, cohesiveness and fish flavor. The fishburger with MSM from washed carcass was the least acceptable, because of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcass; Filleting by-product; Sensory profile; Sensory acceptance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100517

Characterization of native and oxidized starches of two varieties of Peruvian carrot (Arracacia xanthorrhiza, B.) from two production areas of Paraná state, Brazil
Provedor de dados: 52
Autores: Matsuguma,Luciana Shizue; Lacerda,Luiz Gustavo; Schnitzler,Egon; Carvalho Filho,Marco Aurélio da Silva; Franco,Célia Maria Landi; Demiate,Ivo Mottin.
Two commercial varieties of Peruvian carrot ('Amarela de Carandaí' and 'Senador Amaral') were processed into flour, starch and bagasse and chemically evaluated. The starch was extracted, modified with H2O2 and characterized by the physicochemical methods. By using the methylene blue dyeing, the granules of the modified starches showed intense blue color. The carboxyl content, the reducing power and the amount of the water liberated from the pastes after the freeze-thawing were higher for the oxidized starches and their pastes were clearer than those of the native starches of the two varieties from the two production areas. The RVA viscoamylography showed that the modified starches had lower viscosities with differences between the varieties. In the thermal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peruvian carrot varieties; Starch; Hydrogen peroxide; Technological properties.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300022

Chlorophyll stability in yerba maté leaves in controlled atmospheres
Provedor de dados: 52
Autores: Morawicki,Rubén O.; Schmalko,Miguel E.; Känzig,Rodolfo G.
The objective of this research was to investigate the stability of chlorophyll in yerba maté leaves in controlled atmospheres of CO2/air mixtures and different water activities at 25°C.Two levels of water activity were selected corresponding to saturated salt solutions of LiCl (a w=0.113) and MgCl2(a w=0.330) and three levels of CO2/air mixtures (0/100,20/80 and 40/60). The chlorophyll content was evaluated using a liquid chromatography HPLC technique. Experimental values varied between 2.16 and 0.61 mg/g of dry matter. For each sample, 5 determination were made during 58 days. Experimental values were fitted to an equation describing a first order reaction. In all cases, the agreement was good with P&lt;3 10-3. The initial concentration of chlorophyll...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba maté; Chlorophyll stability; Controlled atmosphere.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100012

Effect of polymers associated with N and K fertilizer sources on Dendrathema grandiflorum growth and K, Ca and Mg relations
Provedor de dados: 52
Autores: Regiane Cristina Marcato,Sita; Reissmann,Carlos Bruno; Marques,Renato; Oliveira,Edilson de; Taffarel,Angela Daniela.
This study was conducted to evaluate the effect of polymer used with different nitrogen and potassium sources on the growth and nutrition of chrysanthemum (Dendranthema grandiforum, var. "Virginal") and on chemical characteristics of substrate. Two substrates were evaluated: 1) composite with 50 % organic soil, 45 % sand, and 5 % composted tobacco; 2) plow layer soil (0-20 cm depth; red oxisol typical dystrophic). The experimental design was a factorial (4x2x2) and included four polymer rates (0, 1, 2, and 4 g kg-1of substrate), two nitrogen ((NH4)2SO4 and (H2N)2CO), and two potassium (KCl and K2SO4) sources. Dry biomass, flower number, and concentration of K, Ca, and Mg were evaluated. Inverse relationships between polymer rates and plant biomass,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrogel; Polyacrylamide; Chrysanthemum; Fertilizer; Macronutrients.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300002

Compositional changes of proteins and amino acids in germinating coffee seeds
Provedor de dados: 52
Autores: Shimizu,Milton Massao; Mazzafera,Paulo.
Endosperm is the main reserve tissue in coffee seeds. Coffee (Coffea arabica L.) seeds were germinated for six weeks and qualitative and quantitative changes in amino acids and proteins were investigated. The total content of free amino acids were reduced during germination, however, protein content remained constant. SDS-PAGE profiles showed that legumin-like proteins became less stained in the last weeks. Asparagine, glutamic acid, aspartic acid, alanine and lysine were the major free amino acids, although serine and glutamine were also significant. Except for tyrosine, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed glutamic acid/glutamine and glycine as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Coffea arabica; Endosperm; Germination; Protein; Seeds.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300003

Majoid crabs community (Crustacea: Decapoda) from infralittoral rocky/sandy bottom of Anchieta Island, Ubatuba
Provedor de dados: 52
Autores: Biagi,Renata; Melo,Gustavo A. S.
The aim of the present study was to characterize the composition of Majoidea crabs inhabiting the infralittoral rocky/sandy bottom of Anchieta Island (Ubatuba), from north coast of São Paulo State. Crabs were collected monthly during 1999 and 2000 by using SCUBA and cages. The occurrence of eight species of majoids (Epialtus brasiliensis, Mithraculus forceps, Mithrax braziliensis, Mithrax hispidus, Mithrax tortugae, Pelia rotunda, Pitho lherminieri and Stenorhynchus seticornis) was registered and corresponded to 24.2% of total of majid crabs recorded in the coast of São Paulo State. Mithraculus forceps, Mithrax tortugae and Stenorhynchus seticornis were most abundant in samples and constituted populations that belonged to the brachyuran community of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachyura; Majoidea; Diversity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200015

Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum
Provedor de dados: 52
Autores: Zia,Muhammad Anjum; Riaz,Ayesha; Rasul,Samreen; Abbas,Rao Zahid.
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose oxidase; Antimicrobial; Aspergillus niger; Penicillium notatum.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010

Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers
Provedor de dados: 52
Autores: Sindhu,Raveendran; Ammu,Balakrishnan; Binod,Parameswaran; Deepthi,Sreelatha K.; Ramachandran,K. B.; Soccol,Carlos Ricardo; Pandey,Ashok.
The aim of this work was to study the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source. Effect of various process parameters on PHB production such as glycerol concentration, inoculum size and pH of the medium were optimized. Characterization of extracted PHB was carried out by FT-IR, ¹H and 13C NMR. Results showed that the bacterial culture accumulated about 31% PHB in crude glycerol medium. The extracted PHB was blended with other polymers to improve its physical characteristics. The thermal properties of the polymer like melting temperature (Tm) and heat of fusion (ΔHf) were determined using DSC.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxybutyrate; Bacillus sphaericus; Polymer blends; Crude glycerol; Biodiesel industry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400019

Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis
Provedor de dados: 52
Autores: Pelozo,Maria Inez de Godoy; Cardoso,Mara Lane Carvalho; Mello,João Carlos Palazzo de.
The present work was designed to quantify the caffeine and total polyphenols in the extractive solution and the granulated form from the seeds of Paullinia cupana var. sorbilis, by the spectrophotometric method. The method showed linearity for the caffeine and polyphenols in the range of 5-25 µg/ml and 2.4-5.6 µg/ml respectively. The solutions of the semipurified fraction (EPA) and granulated form (GRA) showed linear responses in the range of 0.288-0.672 and 0.4-1.2 µg/ml, respectively. The precision and accuracy were determined for the EPA solution at a concentration of 100 µg/ml. The spectrophotometric method performed well in quantifying the caffeine and total polyphenols.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeine; Paullinia cupana; Total polyphenols; Spectrophotometric quantification assay.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300002

Characterization of the Relationship of CDKL5 with MeCP2 and Dnmt1 in PrimaryRat Cortical Neurons
Provedor de dados: 52
Autores: Yi,Zhi; Yang,Wenxu; Ma,Yinan; Qi,Yu; Pan,Hong.
ABSTRACT Cyclin-dependent kinase-like 5 (CDKL5) is a protein kinase that is homologous to mitogen-activated protein kinases (MAPKs) and cyclin-dependent kinases (CDKs). Mutations in the CDKL5 gene cause X-linked infantile spasms and phenotypes that overlap with that of Rett syndrome, which is a neurodevelopmental disorder caused primarily by mutations in the methyl CpG binding protein 2 gene (MECP2). Previous studies in transfected cell lines showed that CDKL5 interacts with MeCP2 and DNA (cytosine-5)-methyltransferase 1 (Dnmt1). However, little is known about the relationships of CDKL5 with interacting proteins in primary neuronal cultures. In this study, we investigated the expression patterns of CDKL5, MeCP2 and Dnmt1, and their interaction in cultured...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDKL5; MeCP2; Dnmt1; Neurodevelopment; Primary neurons.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100421

Macroinfauna of Six Beaches near Guaratuba Bay, Southern Brazil
Provedor de dados: 52
Autores: Barros,Francisco; Borzone,Carlos Alberto; Rosso,Sergio.
Benthic macroinfauna of six beaches near to Guaratuba Bay, Paraná, Brazil were studied in summer and winter of 1994. Sampling stations were distributed along a transect, from upper of the drift line to depths of nearly 3 m behind the first break of the surf zone. Biological and sedimentological samples, slope, wave height and period were recorded. Subtidal samples were collected by scuba diving. The beaches varied from extremely inclined, composed of coarse sediments, to low inclined, composed of fine and very fine sands. The polychaete Scolelepis squamata, was the numerically dominant species in those beaches composed by fine to very fine sand in the subaereal profile. The echinoderm Mellita quinquiesperforata occurred in the last stations of the surf...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macroinfauna; Sandy beaches; Zonation; Morphodynamics; Paraná; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400005

Drug interaction with radiopharmaceuticals: effect on the labeling of red blood cells with technetium-99m and on the bioavailability of radiopharmaceuticals
Provedor de dados: 52
Autores: Gomes,Maria Luisa; Oliveira,Marcia B. Nunes de; Bernardo-Filho,Mario.
The evidence that natural and synthetic drugs can affect radiolabeling or bioavailability of radiopharmaceuticals in setting of nuclear medicine clinic is already known. However, this drug interaction with radiopharmaceuticals (DIR) is not completely understood. Several authors have described the effect of drugs on the labeling of blood elements with technetium-99m (99mTc) and on the biodistribution of radiopharmaceuticals. When the DIR is known, if desirable or undesirable, the natural consequence is a correct diagnosis. However, when it is unknown, it is undesirable and the consequences are the possibility of misdiagnosis and/or the repetition of the examination with an increase of radiation dose to the patient. The possible explanation to the appearance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug interaction; Blood elements; Radiopharmaceuticals; Bioavailability.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500020

Pandan (Pandanus sp), Rotan (Calamus sp), and Rengas (Gluta sp) from Kajuik Lake, Riau Province, Indonesia
Provedor de dados: 52
Autores: Roslim,Dewi Indriyani.
ABSTRACT Pandan (Pandanus sp), Rotan (Calamus sp), and Rengas (Gluta sp) are the three most important plants growing at Kajuik Lake, Langgam, Riau Province, Indonesia; however, their species names have not been identified. This study aimed to identify their species names using nuclear internal transcribed spacer (ITS) and psbA-trnH intergenic spacer sequences. The method employed was DNA isolation from fresh leaves, PCR using primer pairs of ITS region for Pandanus sp and psbA-trnH intergenic spacer for Calamus sp and Gluta sp, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.0. Pandanus tectorius was the only one accession that was similar to Pandanus sp with the identity was 90%, however the query cover was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calamus sp; Gluta sp; ITS; Pandanus sp; PsbA-trnH intergenic spacer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100408

Rearing Chrysomya megacephala on artificial diets composed of varying concentrations of albumin
Provedor de dados: 52
Autores: Mendonça,Paloma Martins; Queiroz,Margareth Maria de Carvalho; d'Almeida,José Mario.
Larvae of the blowfly Chrysomya megacephala were reared on an artificial diet composed of five different concentrations of albumin (2, 4, 6, 8 and 10%) and the control group was fed on putrid bovine meat. No larvae developed in the 2 and 10% albumin concentrations. The period from newly hatched larvae to adults reared on 4, 6 and 8% albumin was 13.1, 13.1 and 13.6 days, respectively, whereas for the control group, it was 11.2 days. Concentrations of 4, 6 and 8% albumin proved viabile for larval periods of 29.3, 44.0 and 57.3%, respectively, whereas for the control group, it was 77.3%. Pupal viability was 77.3, 36.4 and 83.7%, while for the control group, it was 84.5%; the newly hatched larvae to adult viability was 21.3, 16.0 and 48.0%, respectively, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blowfly; Calliphoridae; Development; Diptera; Protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200020

Skin Doses on the Lens for Temporomandibular Joint Exam in Cone Beam Computed Tomography
Provedor de dados: 52
Autores: Oliveira,Marcus Vinicius Linhares de; Andrade,Marcos Ely Almeida; Batista,Wilson Otto; Campos,Paulo Sergio Flores.
ABSTRACT The aim of this study was to evaluate the kerma at the surface of the lens in TMJ CBCT and to derive the equivalent dose. An anthropomorphic phantom of the head and neck manufactured by Radiation Support Devices (model RS-230) was used. The dosimetric measurements were obtained by using fourteen thermoluminescent dosimetry (TLD) dosimeters (LiF: Mg, Ti), divided in two pairs (one pair for each eye) and positioned on the surface of the phantom, per scanner evaluated. The tomographic images were acquired in three types of CBCT equipment (CS 9000, Gendex GXCB 500 and i-CAT). Values of equivalent dose obtained were: 5.82 mSv (CS 9000); 5.38 mSv (Gendex GXCB 500) and 7.98 mSv (i-CAT), which varied in accordance with the scanner and the exposure factors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cone beam computed tomography; Temporomandibular joint; Radiation dosimetry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600886

Prediction of genotypic values and estimation of genetic parameters in common bean
Provedor de dados: 52
Autores: Chiorato,Alisson Fernando; Carbonell,Sérgio Augusto Morais; Dias,Luiz Antônio dos Santos; Resende,Marcos Deon Vilela de.
Eighteen common bean (Phaseolus vulgaris L.) genotypes were evaluated in 25 environments of the state of São Paulo in 2001 and 2002. The estimation of genetic parameters by the Restricted Maximum Likelihood (REML) and the prediction of genotypic values via Best Linear Unbiased Prediction (BLUP) were obtained by software Selegen-REML/BLUP. The estimate of the broad-sense heritability was low for the grain yield (0.03), since it took individual plots into consideration and was free of the effects of interaction with years, cultivation periods and site. Nevertheless, the heritability at the level of line means across the various environments was high (0.75), allowing a high accuracy (0.87) in the selection of lines for planting in the environment mean. Among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: REML; BLUP; Phaseolus vulgaris; Mixed Models.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300005

Aqueous Extract of Terminalia chebula Induces Apoptosis in Lung Cancer Cells Via a Mechanism Involving Mitochondria-mediated Pathways
Provedor de dados: 52
Autores: Wang,Meiling; Yang,Limin; Ji,Musi; Zhao,Pengwei; Sun,Peng; Bai,Ruixia; Tian,Yunpeng; Su,Liping; Li,Cunbao.
The current study was designed to evaluate the aqueous extract of Terminalia chebula activity, and the main pathway was detected on lung cancer by extracts of T. chebula. Aqueous extract of T. chebula was separated using a zeolite, and five fractions of T. chebula extract were obtained and analyzed by ultraviolet (UV) and infrared (IR) spectroscopy. Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods against human lung cancer (A549) and mouse lung cancer cell line LLC. T. chebula acts by regulating the Bcl-2 family protein-mediated mitochondrial pathway detected by western blot. Fraction 4 of the T. chebula extract showed much function and was thus studied further. Fraction 4 increased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T. chebula; Aqueous extract; Infrared spectroscopy; Apoptosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200208

Biochemical biomarkers of exposure to deltamethrin in freshwater fish, Ancistrus multispinis
Provedor de dados: 52
Autores: Assis,Helena Cristina da Silva de; Nicareta,Lilian; Salvo,Ligia Maria; Klemz,Claudio; Truppel,Jessé Henrique; Calegari,Rossana.
This study aimed to determine the effect of sublethal doses of deltamethrin, using biochemical biomarkers as activities of cholinesterase (ChE), ethoxyresorufin-O-deethylase (EROD) and the Na+K+- ATPase and levels of total cytochrome P450 (CYP450). Fishes received sublethal doses of deltamethrin and were sacrificed after 96 h of exposure. Samples of gills, heart, brain, liver and muscle were collected for enzymatic analyses. Deltamethrin inhibited the activity of the gills and heart Na+ K+-ATPase, induced the liver total CYP450, as well as the liver EROD activity. The activity of the ChE was not inhibited by deltamethrin. Deltamethrin altered the hepatic metabolism and the normal ionic flux in Ancistrus multispinis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deltamethrin; Cytochrome P450; Fish; Ancistrus multispinis; Biomarker.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600012

Isoflavones in processed soybean products from Ecuador
Provedor de dados: 52
Autores: Genovese,Maria Inés; Davila,Jorge; Lajolo,Franco M.
Soy products produced in Ecuador, with a local developed and cultivated soybean variety (INIAP 306), were analysed for isoflavone content and profile. The products presented high total isoflavone contents, varying from 53 to 106 mg/100 g (wet basis, expressed as aglycones), the lowest content being for okara and the highest for the low fat soybean flour obtained by extrusion cooking of the seeds at the field moisture. Soy nuts showed the same content of isoflavones than the seeds, but with lower amounts of malonylglycosides and higher of the deesterified beta-glycosides. The malonylglycosides were the predominant form of the isoflavones in the flours, and the beta-glycosides in soymilk and textured soy protein. Genistein derivatives were the compounds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecuadorian soybean products; Isoflavone content; Isoflavone profile.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600020

Characterization of Seed Oils of Pterodon emarginatus Vogel (Sucupira) Obtained by Different Extraction Methods
Provedor de dados: 52
Autores: Costa-Singh,Tainara; Jorge,Neuza.
Abstract The present study aims to evaluate the influence of extraction methods on the quality of bioactive compounds and antioxidant capacity in Pterodon emarginatusVogel (sucupira) oils. The oils were extracted from the sucupira seeds by Soxhlet and by the extraction system of Bligh &amp; Dyer. The oils were analyzed as to fatty acid profile, tocopherols, phytosterols, carotenoids, total phenolics, and total antioxidant capacity. The extraction by Soxhlet showed better yield of total lipids and was more efficient to extract tocopherols, phytosterols, and carotenoids, besides presenting better antioxidant activity by DPPH•. However, this method showed insufficient capacity to extract the polar lipid components of the sample, as evidenced by the low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Bioactives; Extraction; Oxidative stability; Chromatography.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100506

Growth curve and development of the internal calli structure of Eucalyptus camaldulensis Dehn
Provedor de dados: 52
Autores: Mendonça,Evânia Galvão; Paiva,Luciano Vilela; Stein,Vanessa Cristina; Pires,Marinês Ferreira; Santos,Breno Régis; Pereira,Fabricio José.
The objective of this work was to elucidate the growth curve of Eucalyptus camaldulensis Dehn. calli analyzing their anatomical modifications. A sigmoid aspect of the growth curve of the calli fresh matter was observed, with five different phases (lag, exponential, linear, deceleration and decline). In the lag phase, the highest growth percentage 87%, was observed, which reduced during the evaluation period to 17% in the linear phase. As for the anatomical analyses, cellular multiplications was observed during the lag and exponential phases and increase in cell size during the linear phase, promoting the calli volume growth and the establishment of the globular conformation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro cultivation; Organogenesis; Anatomy; Astra blue; Safranin; Forestry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600012

Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
Provedor de dados: 52
Autores: Loera,Octavio; Córdova,Jesús.
A diploid strain (D4) isolated via parasexual recombination between two Aspergillus niger xylanase overproducing mutants was characterised in terms of enzyme production and catabolite repression by glucose. This strain increased xylanase production (607 nkat/ml), which was nearly 100% higher than titers achieved by the wild type strain (305 nkat/ml) and 28% higher than the best mutant used to induce parasexual cycle. Diploid D4 was also less sensitive to carbon catabolite repression by glucose, since xylanolytic activity was detected under conditions normally repressing production by the wild type strain. No decrease in maximal xylanase levels was observed in the presence of glucose for diploid D4.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbon repression; Xylanase production; Mutants; Parasexual cross.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200006

Bioefficacy of pectolinaringenin from Clerodendrum phlomidis Linn. F. against Anopheles stephensi and bhendi fruit borer, Earias vittella fab.
Provedor de dados: 52
Autores: Muthu,Chelliah; Baskar,Kathirvelu; Duraipandiyan,Veeramuthu; Ignacimuthu,Savarimuthu; Al-Dhabi,Naif Abdullah.
Larvicidal activity of pectolinaringenin from Clerodendrum phlomidiswas evaluated against Anopheles stephensi and antifeedant, larvicidal and growth inhibitory activities were evaluated against Earias vittella. Pectolinaringenin exhibited larvicidal activity of 100 and 98.24% against 2nd and 4th instar larvae of Anopheles stephensi at 5ppm concentration. It exhibited LC50 values of 0.35 and 0.55 ppm for 2nd and 4th instar larvae, respectively. At 100 ppm concentration, pectolinaringenin exhibited maximum antifeedant activity of 74.00% and larvicidal activity of 89.98%. The LC50 values were 36.2 and 10.23 ppm for antifeedant and larvicidal, respectively. The compound completely prevented the adult emergence at 50 and 100 ppm concentrations. This is the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anopheles stephensi; Adult emergence; Fraction; Flavonoid; Effective concentration.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300358

Pelletization of seeds of Raphanus sativus L. cv. Redondo Gigante with graphite for germination under water stress conditions
Provedor de dados: 52
Autores: Freitas,Nobel Penteado; Takaki,Massanori.
The effect of water stress on germination of radish seeds is dependent on the presence of light. The effect of pelletization of radish seeds with powdered graphite on the tolerance to water stress under light was analysed. White light and far-red lights were filtered by graphite increasing slightly the tolerance of pelleted seeds to water stress of -0.77MPa. Although red light also inhibited seed germination the graphite had no effect. We propose the pelletization of seeds of Raphanus sativus, at least in cv redondo gigante with graphite as a pratice before planting to increase the tolerance to water stress.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pelletization; Raphanus sativus; Seed germination; Water stress.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500016

Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis
Provedor de dados: 52
Autores: Chaud,Luciana Cristina Silveira; Silva,Débora Danielle Virgínio da; Mattos,Rafael Taino de; Felipe,Maria das Graças de Almeida.
Oat hull hemicellulosic hydrolysate obtained by diluted acid hydrolysis was employed as fermentation medium for Pichia stipitis cultivation. A comparison between the use of treated hydrolysate with 1% activated charcoal to reduce the toxic compounds generated during the hydrolysis process and untreated hydrolysate as a control was conducted. In the cultures using treated hydrolysate the total consumption of glucose, low xylose consumption and ethanol and glycerol formation were observed. The medium formulated with untreated hydrolysate showed morphological cell modifications with consequently cell death, no ethanol formation and formation of glycerol as byproduct of fermentative process, probably as a response to stressful conditions to yeast due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pichia stipitis; Hemicellulosic hydrolysate; Oat hull; Ethanol; Activated charcoal.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500017

Morphology of female reproductive tract of the predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) fed on different diets
Provedor de dados: 52
Autores: Lemos,Walkymário de Paulo; Ramalho,Francisco de Souza; Serrão,José Eduardo; Zanuncio,José Cola.
The morphology of the reproductive tract of Podisus nigrispinus (Dallas) females fed with Alabama argillacea (Hübner) larvae, artificial diet, Tenebrio molitor L. larvae or Musca domestica L. larvae were studied. The reproductive tract of females of this species presented yellow coloration and independent of the diet, each ovary had seven ovarioles joined through terminal filaments and forming a bunch shape structure. The histological data revealed that the ovary of P. nigrispinus was of meroistic telotrophic type, with each individual ovariole divided in a terminal filament, a tropharium (trophic chamber), a vitellarium, and a pedicel. The prey type affected the development and morphometry of these structures. Females of P. nigrispinus fed with 3rd or 5th...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Morphology; Ovaries; Predator; Reproduction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100017

Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.)
Provedor de dados: 52
Autores: Ahmadpour,Roghayeh; Zare,Nasser; Asghari-Zakarta,Rasool; Sheikhzadeh,Parisa.
ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Plant growth regulators; Somatic embryogenesis; Wheat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100206

Effects of alternating magnetic field on the metabolism of the healthy and diabetic organisms
Provedor de dados: 52
Autores: Öcal,Işıl; Kalkan,Tunaya; Günay,İsmail.
The aim of this study was to determine the effects of alternating magnetic field on the metabolism of the healthy and diabetic rats. The rats were divided into two experimental groups. Magnetic fields of 5 and 8mT intensity was applied to the first group of rats for 3 weeks (C5mT, CMF5mT, C8mT and CMF8mT). The second group was identified as diabetics group and 5mT was applied (D5mT and DMF5mT). The control groups (C5mT, C8mT, D5mT) of applied magnetic fields were placed in the same cage of the rats in experimental groups that have the same conditions but no pass current. The weights of rats exposed to magnetic fields in experimental groups were increased to initial weight; the plasma glucose levels were decreased in the blood parameters of the healthy and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternating magnetic field; Diabetic rats; Blood plasma parameters; Physiological conditions.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300011

Working Optimally with Serial Sections in Glycol Methacrylate Resin
Provedor de dados: 52
Autores: Leitão,Carlos André Espolador.
ABSTRACT The present work presents an optimized form for distributing serial sections in a glycol methacrylate slide set. This consists of filling the first row with sections from each slide, thus proceeding in the same way with the other rows. Therefore each slide will contain sections of three distinct sample segments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytology; Histology; Historesin; Microscopy; Plant anatomy; Technique.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100416

Structural Behavior of SFRC Beams strengthened with GFRP Laminates: An Experimental and Analytical Investigation
Provedor de dados: 52
Autores: Mariappan,M.; Raghunath,P.N.; Sivaraja,M.
ABSTRACT This paper presents the experimental results of a reinforced concrete beams (RC) strengthened with internal steel fibers (SF) and external glass fiber reinforced polymer laminates (GFRP). The research work studied the load carrying capacity, deformation, crack width and ductility of the reinforced concrete beams strengthened with different steel fiber ratios and steel fiber reinforced concrete beams strengthened with three different glass fiber reinforced polymer laminates of two different thickness. The experimental results clearly shows that incorporating steel fibers in to the reinforced concrete beams reduced the crack width and distribute the crack evenly and also increases the bonding between tension face of the beam with glass fiber...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RC; FRC; GFRP; SFRC; Ductility.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300407

The 3D CFD study of gliding swimmer on passive hydrodynamics drag
Provedor de dados: 52
Autores: Mantha,Vishveshwar Rajendra; Marinho,Daniel Almeida; Silva,Antonio Jose; Rouboa,Abel Ilah.
The aim of this study was to analyze the effect of depth on the hydrodynamic drag coefficient during the passive underwater gliding after the starts and turns. The swimmer hydrodynamics performance was studied by the application of computational fluid dynamics (CFD) method. The steady-state CFD simulations were performed by the application of k - omega turbulent model and volume of fluid method to obtain two-phase flow around a three-dimensional swimmer model when gliding near water surface and at different depths from the water surface. The simulations were conducted for four different swimming pool size, each with different depth, i.e., 1.0, 1.5, 2.0 and 3.0 m for three different velocities, i.e., 1.5, 2.0 and 2.5 m/s, with swimmer gliding at different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Computational Fluid Dynamics (CFD); Passive Drag; Starts; Turns; Streamline Gliding Position; Glide depth.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200020

Leaf morphology of 89 tree species from a lowland tropical rain forest (Atlantic forest) in South Brazil
Provedor de dados: 52
Autores: Boeger,Maria Regina Torres; Alves,Luiz Carlos; Negrelle,Raquel Rejane Bonatto.
We examined the leaf morphology and anatomy of 89 tree species growing in an area of coastal Atlantic Forest in South Brazil. The majority of the species (&gt; 75%) had small (notophyll and microphyll) elliptical simple leaves with entire margins. These leaves presented a typical anatomical structure consisting of a single epidermal cell layer, single palisade parenchyma cell layer, and spongy parenchyma with 5 to 8 cell layers. The sclerenchyma was limited to the vascular bundles. The majority of the tree species (91%) had leaves with mesomorphic characteristics. Few species depicted leaves with xeromorphic features as would be expected in such oligotrophic sandy soil. These mesomorphic features appeared to be associated to high efficiency mechanisms for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf morphology; Leaf anatomy; Lowland tropical forest; Atlantic Forest.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600013

Induction of an antigen specific gut inflammatory reaction in mice and rats: a model for human Inflammatory Bowel Disease
Provedor de dados: 52
Autores: Teixeira,Gerlinde Agate Platais Brasil; Antunes,Danielle Mota Fontes; Castro Júnior,Archimedes Barbosa de; Costa,Janilda Pacheco da; Paschoal,Patrícia Olaya; Campos,Sylvia Maria Nicolau; Pedruzzi,Monique de Morais Bitetti; Araújo,Vinicius da Silva; Andrade,Luis Antônio Botelho; Moreno,Silvana Ramos Farias; Nobrega,Alberto Felix Antonio da; Cardoso,Gilberto Perez.
Food allergy is an adverse reaction that occurs in susceptible people when they eat sensitizing foods and is one of the causes of Inflammatory Bowel Disease (IBD). The effort to understand the induction process of these diseases is important as IBD is increasing worldwide, including in Brazil. The aim of this study was to develop an experimental antigen specific inflammatory process of the gut of mice and rats, using peanut seeds. Animals were immunized with peanut protein extract before their exposure to the in natura peanut seeds. Results showed that systemic immunization with peanut protein extracts rendered significantly higher antibody titers than control groups and that immunized animals submitted to a challenge diet containing peanuts presented time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food allergy; Peanuts; Inflammatory bowel disease; Rats; Mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300012

Potencial for natural forest regeneration from seed bank in an upper Paraná river floodplain, Brazil
Provedor de dados: 52
Autores: Campos,João Batista; Souza,Maria Conceição de.
The historical process of deforestation was analyzed to evaluate the regeneration potential of forests from soil seed bank of Porto Rico island (53° 15'W and 22° 45'S) in the upper Paraná river floodplain. Remnant forest fragments were identified and measured and the structure of arboreous vegetation and the composition of the seed bank of the forests and grassland of the island were evaluated. Results showed a fast process of deforestation with critical levels of forest: the remaining twice fragments represented only 5.98% of the total surface of the island. Disturbance by cattle raised on the island continuously degraded the fragments (backward succession), while expansion of areas with pasture favored severe impoverishment of the seed banks flora. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deforestation; Alluvial forest; Floodplain; Paraná river; Brazil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400018

Anatomical study of Racosperma (ex-Acacia) mangium tissues cultured in vitro
Provedor de dados: 52
Autores: Takemori,N. K.; Marschner,R.; Quoirin,M.; Bona,C.; Zanette,F.
In order to achieve adventitious shoot regeneration from young tissues of Racosperma mangium, several combinations of growth regulators were tested. Kinetin (KIN) and a -naphthalenacetic acid (NAA), thidiazuron (TDZ) and NAA or indole-3-butyric acid (IBA), 4-CPPU and 2,4-dichlorophenoxyacetic acid (2,4-D) were added to basic medium composed of MS salts, MS vitamins and 3% sucrose. Sections of hypocotyls and cotyledons of young seedlings were cultivated on these media. Calli developed on different media and, after two to three months, some of them regenerated organized structures. Histological studies revealed hypertrophy and hyperplasy of medular and cortical parenchyma cells of hypocotyl and cotyledon parenchyma. Formation of meristematic cells and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-CPPU; Leguminosae; TDZ; Tissue culture.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100008

External morphology of the adult of Heraclides anchisiades capys (Hübner, [1809]) (Lepidoptera - Papilionidae) II: Thorax and thoracic appendages
Provedor de dados: 52
Autores: Leite,Luis Anderson Ribeiro; Casagrande,Mirna Martins; Mielke,Olaf Hermann Hendrik.
The purpose of this study was to highlight the morphological components of the thorax and its appendages of the adults of Heraclides anchisiades capys (Hübner, [1809]), seeking a comparative focus with other Papilionoidea and Hesperioidea. The morphology was studied using illustrations and scanning electronic microscope. The most relevant morphological characters were: membranous patagia on prothorax; anepisternum II fused with the pleural wing process II as a small sclerite dorsal to the katepisternum II; 3A present on the forewing, reaching the inner margin on its proximal third; presence of basalare III "pad" and meral suture on coxa III.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Neotropical; Papilioninae; Papilionoidea.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600017

Response surface analysis on the effect of temperature and pH on Growth and proteolytic activity of thermophilic Bacillus sp
Provedor de dados: 52
Autores: Gorlach-Lira,Krystyna; Pedroza,Maria Lourdes Vieira; Burdziej-Pokojska,Aleksandra; Rozycki,Henryk; Dahm,Hanna.
Proteolytic activity and cell biomass of thermophilic Bacillus sp. strain were evaluated at various levels of initial pH and temperature by applying response surface methodology. The mineral medium containing yeast extract (0.01%) and starch (1%) was used throughout the experiment. The results of statistical analysis revealed the polynomial model with high coefficient of determination (R² = 0.8) for the biomass and total proteolytic activity of the strain studied. This model showed a satisfactory adjustment of the statistic model with the experimental data. The p values showed that the temperature and pH had significant effect on biomass and proteolytic activity (P&lt;0.05) of strain tested. The highest proteolytic activity (2.333 U/ml/h) of the Bacillus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Proteolytic activity; Response surface methodology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500009

Use of vinasse and sugarcane bagasse for the production of enzymes by lignocellulolytic fungi
Provedor de dados: 52
Autores: Aguiar,Mario Mamede; Ferreira,Luiz Fernando Romanholo; Monteiro,Regina Teresa Rosim.
In this present work, three strains of Pleurotus and Trichoderma reesei were cultivated in media with pre-treated bagasse and vinasse. Cellulolytic and lignolytic activities and biomass production were analyzed. The treatment of the bagasse with 2% H2O2 + 1.5% NaOH + autoclave resulted in a greater fiber breakage increasing the cellulose level up to 1.2 times and decreasing 8.5 times the hemicellulose content. This treatment also resulted in a high lignolytic activity for all cultures utilized. T. reesei produced laccase, peroxidase and manganese-peroxidase in all the treatments, having its manganese-peroxidase activity raging from 1.9 to 4.8 times higher than the basidiomycetes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bagasse; Enzyme; Fungi; Lignocellulolytic; Pleurotus; Sugarcane.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500030

Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification
Provedor de dados: 52
Autores: Silva,Gildo Almeida da; Bernardi,Taís Letícia; Schaker,Patrícia Dayane Carvalho; Menegotto,Morgana; Valente,Patricia.
The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boiling; Freeze-thawing; DNA extraction; Dekkera bruxellensis; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020

Assessment of chromium contamination in the Monte Alegre stream: a case study
Provedor de dados: 52
Autores: Corbi,Juliano José; Santos,Felipe André dos; Zerlin,Ricardo; Santos,Ademir dos; Froehlich,Claudio Gilberto; Trivinho-Strixino,Susana.
The aim of this work was to study the contamination by chromium in the sediments of the Monte Alegre stream and in the larvae of Odonata and the possible impact caused by them on the stream macroinvertebrates community. It was found that chromium contaminated the sediments and the aquatic biota. Although, the stream macroinvertebrates community structure did not appear to be modified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stream macroinvertebrates; Contamination; Chromium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300023

Direct RAPD evaluation of bacteria without conventional DNA extraction
Provedor de dados: 52
Autores: Araújo,Welington Luiz; Angellis,Derlene Attili de; Azevedo,João Lúcio.
The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell suspension was diluted and 2.0 µl were used in reactions of 15 µl. The results showed no significant differences among the RAPD profile of the PCR reactions derived from the boiling and phenol extraction methods, suggesting the utilization of this method for genetic population analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Boiling method; DNA extraction; RAPD.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006

Nuclear medicine imaging technique in the erectile dysfunction evaluation: a mini-review
Provedor de dados: 52
Autores: Ribeiro,Camila Godinho; Moura,Regina; Neves,Rosane de Figueiredo; Spinosa,Jean Pierre; Bernardo-Filho,Mario.
Functional imaging with positron emission tomography and single photon emission computed tomography is capable of visualizing subtle changes in physiological function in vivo. Erectile dysfunction(ED) diminishes quality of life for affected men and their partners. Identification of neural substrates may provide information regarding the pathophysiology of types of sexual dysfunction originating in the brain. The aim of this work is to verify the approaches of the nuclear medicine techniques in the evaluation of the erectile function/disfunction. A search using the words ED and nuclear medicine, ED and scintigraphy, ED and spect and ED and pet was done in the PubMed. The number of citations in each subject was determined. Neuroimaging techniques offer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear medicine; Erectile dysfunction; Positron emission tomography; Single photon emission computed tomography.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600010

Chemical analysis of turmeric from Minas Gerais, Brazil and comparison of methods for flavour free oleoresin
Provedor de dados: 52
Autores: Souza,Cyleni R. A.; Glória,Maria Beatriz Abreu.
Chemical analysis of turmeric (Curcuma longa L) cultivated in eight different cities in the state of Minas Gerais, Brazil was carried out. The levels of curcuminoid pigments varied from 1.4 to 6.14 g/100 g and of volatile oil from 0.97 to 7.55 mL/100 g (dry basis). Samples from Patrocínio, Arinos and Brasilândia contained higher pigment levels compared to the others. The sample from Patrocínio contained the highest volatile oil content. The mean levels of ethyl ether extract, protein, fiber, ash and starch were 8.51, 7.01, 7.22, 7.81 and 39.87 g/100 g dry basis, respectively. Laboratory extraction of flavour free oleoresin was performed in triplicate. A higher yield of pigment in the oleoresin was obtained when the volatile oil was extracted with water...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turmeric; Curcumin; Quality; Flavour free oleoresin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200008

Influence of substrate humidity on desiccation resistance capacity in Subulina octona (Mollusca, Subulinidae)
Provedor de dados: 52
Autores: Dias,Roberto Júnio Pedroso; Bessa,Elisabeth Cristina de Almeida; D'Ávila,Sthefane.
The aim of this study was to verify the influence of substrate humidity on desiccation resistance of Subulina octona, continuously exposed, for 48 h, to the temperature of 35 °C. Forty individuals conditioned in 2 plastic boxes, one box containing black earth humidified with 40 ml water and the other one containing dry black earth were used. The retraction of cephalopodal mass inside the shell and the number of dead individuals was evaluated. Snails from dry and humid substrate boxes showed cephalopodal mass retraction of 9.9 cm±2.88 and 2.78 cm±3.17, respectively, after 12 h of exposure; 8.55 cm±1.3 and 6.33 cm±2.91, after 24 h; 11.07 cm±4.19 and 7.27 cm±2.7, after 36 h, 9.12 cm±2.15 and 6.47 cm±2.11, after 48 h. Every individual of dry substrate box died...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Land snail; Desiccation; Cephalopodal mass retraction; Substrate; Humidity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100016

Variation in the ureide content of Jack Bean during the reproductive stages in response to nitrate
Provedor de dados: 52
Autores: Camargos,Liliane Santos; Aguiar,Leandro Ferreira; Carmezini,José Antônio; Azevedo,Ricardo Antunes.
Nitrogen is frequently the limiting mineral nutrient for plant productivity and it is essential to obtain an understanding of how this element is assimilated and its metabolism regulated, in crop plants. The objective of this work was to study nitrogen metabolism in the tropical legume Canavalia ensiformis, a plant species used as a green manure. The nitrate dose provided changed the concentrations of ureides exported via the xylem, whilst the developmental stage alterations demonstrated to influence the form of ureide exported via the xylem. Considering the content of ureides as an indicator of N-fixation status, it could be concluded that N-fixation was affected in the presence of nitrate in C. ensiformis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrogen metabolism; Allantoin; Allantoic acid; Canavalia ensiformis; Ureides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300009

In Silico Identification of MicroRNAs with B/CYDV Gene Silencing Potential
Provedor de dados: 52
Autores: Bouallègue,Maryem; Bouktila,Dhia; Mezghani-Khemakhem,Maha; Capy,Pierre; Makni,Mohamed.
ABSTRACT Computational investigation of a set of publicly available plant microRNAs revealed 19 barley- and other plants-encoded miRNAs and their near-complement reverse sequences (miRNA*) that have potential to bind all B/CYDV open reading frames (ORFs) except ORF0 and ORF6. These miRNAs/miRNAs*, their binding positions and targets are discussed in the context of biological protection of cereals against B/CYDV, based on antiviral silencing.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barley/Cereal Yellow Dwarf virus (B/CYDV); MicroRNA; Gene silencing; Host defense.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100437

Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
Provedor de dados: 52
Autores: Bruno,Laura Maria; Lima Filho,José Luiz de; Castro,Heizir Ferreira de.
Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida lipase; Mucor lipase; Hybrid matrix; Immobilization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003

Responses of Diabrotica speciosa to a semiochemical trap characteristics
Provedor de dados: 52
Autores: Arruda-Gatti,Iara Cintra de; Silva,Flávia Augusta Cloclet da; Ventura,Maurício Ursi.
Responses of Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae) to a semiochemical trap characteristics were investigated in the field. The trap consisted of plastic bottles with several perforations (0.5 cm diameter and 2.0 cm distance each other) and containing Lagenaria vulgaris L. (Cucurbitaceae) powder as cucurbitacin (arrestant and phagostimulant) source (0.28%). In common bean fields, transparent green traps caught significantly more males and females beetles than yellow, transparent and white traps. Yellow traps caught significantly more females than white traps. Transparent green and yellow traps baited with the volatile attractant 1,4-dimethoxybenzene caught 4.08 and 2.72 times more beetles than unbaited, respectively. Higher number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Kairomones; Diabroticite; Cucurbitacin; Volatile attractant.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700015

Efeito de sedimentos contaminados sobre a excreção de nitrogênio do camarão Penaeus paulensis
Provedor de dados: 52
Autores: Moraes,Rosane B.C.; Pfeiffer,Wolfgang C.; Guimarães,Jean R.; Borges,Alexandre Luiz N.; Campos,Alexandre N.
Physiological response (ammonia excretion) of the shrimp Penaeus paulensis exposed to contaminated sediments of Guanabara Bay and Sepetiba Bay (Rio de Janeiro - Brazil), was monitored for 52 days. The toxicity tests were conducted in aquarium with a water feedback system and a sediment layer of 2 cm. The sediment from Saco do Engenho (Sepetiba Bay) was the most toxic, affecting the excretion of P. paulensis. In sediment from Sepetiba Bay the ammonia excretion was significantly enhanced after 10 days (S. Engenho) and 52 days (I. Madeira) of exposition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sediment; Toxicity; Excretion; Penaeus.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400012

Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification
Provedor de dados: 52
Autores: Wu,Xulong; Xiao,Lu; Wang,Yin; Yang,Zexiao; Yao,Xueping; Peng,Bin.
ABSTRACT A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). A set of LAMP primers was designed based on the sequence of the ASFV gene K205R. Reaction temperature and time were optimized to 64 oC and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or visually with the addition of fluorescent dye. The detection limit of the LAMP assay was approximately 6 copies of the target gene per microliter, 100 times more sensitive than conventional PCR. LAMP is a simple and inexpensive molecular assay format for ASFV detection. To date, African swine fever has not been reported in China. LAMP can be used to monitor ASFV spread into China,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African swine fever virus; LAMP; K205R gene; Molecular biology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200400

Determination of metabolizable energy value of corn with different average geometric diameters for european quails (Coturnix coturnix coturnix)
Provedor de dados: 52
Autores: Rezende,Marcelo José de Mello; Torres,Alessandro Figueiredo; Murata,Luci Sayori; Garcia,José Américo Soares; McManus,Concepta Margaret.
Metabolizable energy (ME) of corn with different geometric diameters was determined in European quail with 26 days of age with 124 g of mean live weight, using the Total Collection of Excreta Method. One hundred and twenty five quails were divided in five treatments, five replications with five quails each, with one treatment used to determine endogenous losses. Values of Average Geometric Diameter (AGD) of the corn were 600, 800, 1000 and 1200 µm. ME of corn was not affected by AGD. Average values were 3079, 3274, 3300, 3137 Kcal/kg respectively for apparent metabolizable energy, corrected apparent metabolizable energy, true metabolizable energy, corrected true metabolizable energy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolizable apparent energy; Metabolizable true energy; Particle size; Total collections of excreta.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400022

Alkaloids and triterpene from Almeidea coerulea (Nees and Mart.) a. St.-Hil. and anti-leishmanial activity
Provedor de dados: 52
Autores: Cortez,Lucia Elaine Ranieri; Ferreira,Izabel Cristina Piloto; Lonardoni,Valdrinez Campana; Ferreira,Antonio Gilberto; Vieira,Paulo Cesar; Silva,Maria Fátima das Graças Fernandes da; Fernandes,João Batista; Cortez,Diógenes Aparicio Garcia.
The dichloromethane extract of Almeidea coerulea stems yielded the (11-hydroxyrutaecarpine alkaloid reported for the first time from this species) and the triterpene (28-hydroxy-28, 29-dihydrolupeol). The dictamine, skimianine, sitosterol and stigmasterol were also isolated from methanol extract. Extracellular forms of Leishmania amazonensis (promastigotes) was tested with dichloromethane extract and 28-hydroxy-28, 29-dihydrolupeol with showed anti-leishmanial activity above 0.1 mg/mL and 75µg/mL (inhibited 50% promastigote growth), respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Almeidea coerulea; Rutaceae; Indolopyridoquinazoline alkaloid; Anti-leishmanial activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100008

Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis
Provedor de dados: 52
Autores: Oliveira,Antonio Martins; Oliva Neto,Pedro de.
The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions, the RNA yield was 89.7%, resulting in 51.3% of dehydrated yeast extract with 57.9% protein. The use of 12.2% NH3 at 60ºC after autolysis (8h) and plasmolysis (8h) was not viable due to the reduction in the RNA yield from 89.7to78.4%. On the other hand, the thermal shock at 60ºC for 15 minutes prior to autolysis provided an increase in the yield from 89.7 to 91.4%. The autolysis, including NaCl plasmolysis in the optimized conditions was efficient, economic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autolysis; NaCl plasmolysis; Yeast extract; RNA extraction; Nucleotides.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500019

Hydrology, plankton, and corals of the Maracajaú reefs (Northeastern Brazil): an ecosystem under severe thermal stress
Provedor de dados: 52
Autores: Mayal,Elga Miranda; Neumann-Leitão,Sigrid; Feitosa,Fernando Antônio do Nascimento; Schwamborn,Ralf; Silva,Tâmara de Almeida e; Silva-Cunha,Maria da Glória Gonçalves da.
This study provides baseline information on the hydrological conditions and on the coral and plankton communities at the Maracajaú reef ecosystem (Northeastern Brazil). Studies were performed from February to June 2000, covering the transition from dry to rainy season. In this area, there is an offshore coral reef formation, where corals were observed in loco; the water samples were collected to obtain the hydrological and plankton data. Six scleractinian species were identified. Stable isotope analysis on the carbonate fraction of Favia gravida fragments showed that these corals were under severe thermal stress. Chlorophyll-a varied from 1.1 to 9.3 mg m-3, with higher values during the rainy season. Average zooplankton wet weight biomass were 117.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coral reefs; Hydrology; Plankton; Thermal stress; Human impacts.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300019

Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn
Provedor de dados: 52
Autores: Ono,Elisabete Yurie Sataque; Silva,Marcelo da; Ribeiro,Ricardo Marcelo Reche; Ono,Mario Augusto; Hayashi,Luciana; Garcia,Glauco Tironi; Hirooka,Elisa Yoko.
In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thin-Layer Chromatography; Spectrofluorimetry; BGYF; Aflatoxins; Corn; Mycotoxins.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300025

Evaluation of the possible role of ants (Hymenoptera: Formicidae) as mechanical vectors of nematodes and protists
Provedor de dados: 52
Autores: Villani,Fabio; Morini,Maria Santina de Castro; Franco,Marco Antonio; Bueno,Odair Correa.
Nematodes and protists can be transmitted to humans in many ways and little concern has been given to the mechanical transmission by ants. This study aimed at analysing how the eggs of Ascaris lumbricoides and cysts of Entamoeba coli could be mechanically transmitted to the man by Formicidae. Through the experiments using nests of Tapinoma melanocephalum, Linepithema humile and Monomorium pharaonis reared in the laboratory allied to observations of some 17 ant species in an urban park area in Mogi das Cruzes (SP), it was found that L. humile was capable of carrying eggs of A. lumbricoides both in the field and laboratory conditions (1 worker), as well as was Camponotus rufipes (2), Solenopsis saevissima (1) and Acromyrmex niger (1). The cysts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascaris lumbricoides; Entamoeba coli; Ant; Mechanical vector.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500008

Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.)
Provedor de dados: 52
Autores: Rojas-Lorz,Laura; Arrieta-Espinoza,Griselda; Valdez-Melara,Marta; Pereira,Luiz Filipe Protasio; Gatica-Arias,Andrés.
Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Silver nitrate; Somatic embryos; Plant growth regulators; Tissue culture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100408

Anatomical Characters of Leave and Stem of Calea serrata Less., Asteraceae
Provedor de dados: 52
Autores: Camilotti,Júlia Gabriela; Biu,Ciane Costa; Farago,Paulo Vitor; Santos,Vera Lúcia Pereira dos; Franco,Celia Regina Cavichiolo; Budel,Jane Manfron.
The genus Calea belongs to the tribe Heliantheae and presents about 125 species. Calea serrata, popularly known as erva-de-cobra, chá-amargo and quebra-tudo, is an endemic species found in southern Brazil and is used in traditional medicine to treat ulcers and livers problems. The present work aimed to study the pharmacobotanical characters of leaves and stems of C. serrata for quality control purposes. The plant material was processed according to standard methods of light and scanning electron microscopy. Glandular capitate-stalked and capitate-sessile, uniseriate multicellular non-glandular trichome with tapered apical cell, conical non-glandular trichome, isobilateral mesophyll, secretory ducts near the endoderm and circular shape with six ribs in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calea serrata; Anatomical study; Quality control.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600867

Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes
Provedor de dados: 52
Autores: Ghosh,Barnita; Ray,Rina Rani.
Extra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoamylase; Rhizopus oryzae; SSF; Waste utilization.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500003

Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures
Provedor de dados: 52
Autores: Lima,Eleonidas Moura; Rissino,Jorge Dores; Guimarães,Adriana Costa; Overal,David James; Khayat,André Salim; Souza,Patrícia Carvalho de; Christofolini,Denise Maria; Smith,Marília de Arruda Cardoso; Burbano,Rommel Rodríguez.
The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hep-2 cell line; Drifter technique; Mitotic index.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500005

Protective Effect of Carvacrol Against Oxidative Stress and Heart Injury in Cyclophosphamide-Induced Cardiotoxicity in Rat
Provedor de dados: 52
Autores: Cetik,Songul; Ayhanci,Adnan; Sahinturk,Varol.
Possible protective effects of carvacrol (Car) against cyclophosphamide (CP)-induced cardiotoxicity was examined in this study. Experimental groups of the rats were randomly divided into 13 groups,each including seven animals: Group 1 (control) treated with saline; groups 2, 3, and 4 treated with 50, 100, or 150 mg/kg of CP, respectively; group 5 treated with 0.5 mL olive oil; groups 6 and 7 treated with 5.0 and 10 mg/kg of Car, respectively; groups 8, 9, or 10 treated with respective CP plus 5.0 mg/kg of Car; and groups 11, 12, or 13 treated with respective CP plus 10 mg/kg of Car. Serum alanine transaminase (ALT),aspartat transaminase (AST), lactate dehydrogenase (LDH), malondialdehyde (MDA),creatine kinase-MB (CK-MB), total oxidant state (TOS),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclophosphamide; Oxidative stress; Cardiotoxicity; Carvacrol; Antioxidant; Rat.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400569

Neonatally induced diabetes: liver glycogen storage in pregnant rats
Provedor de dados: 52
Autores: Iessi,Isabela Lovizutto; Bueno,Aline; Sinzato,Yuri Karen; Spada,Ana Paula Machado; Rudge,Marilza Vieira Cunha; Heubel,Maricê Thereza Correa Domingues; Damasceno,Débora Cristina.
The aim of this sstudy was to evaluate the liver glycogen storage in pregnant rats presenting neonatal streptozotocin-induced diabetes and to establish a relation with glycemia and insulin levels. Wistar rats were divided in to two groups: 1) Mild Diabetes (STZ) - received streptozotocin (glycemia from 120 to 300 mg/dL), 2) Control - received vehicle (glycemia below 120 mg/dL). At days 0, 7, 14 and 21 of the pregnancy, body weight and glycemia were evaluated. At day 21 of the pregnancy, the rats were anesthetized for blood and liver collection so as to determine insulin and liver glycogen, which showed no changes in the STZ group as compared to the controls. In the STZ group, maternal weight gain were lower as compared to those in the control group....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pregnancy; Rat; Hyperglycemia; Glycogen storage liver; Mild diabetes; Hyperglycemia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200010

Nephroprotective and anti-inflammatory effects of aqueous extract of Melissa officinalis L. on acetaminophen-induced and pleurisy-induced lesions in rats
Provedor de dados: 52
Autores: Müzell,Denise Pereira; Lunardelli,Adroaldo; Leite,Carlos Eduardo; Fagundes,Rodrigo Medeiros; Saciura,Vasyl Custódio; Reichel,Carlos Luiz; Oliveira,Jarbas Rodrigues de; Astarita,Leandro Vieira.
This study assessed the bioactive properties of an aqueous extract of M. officinalis for its anti-inflammatory activity and its protection against hepatic and renal lesions induced by acetaminophen (APAP). Animals pre-treated with the crude extract in pleurisy induced by carrageenan showed a reduction in the amounts of exudate, in the numbers of leukocytes and polymorphonuclear cells. Intragastric administration of the extract for seven days prior to the APAP-induced lesion showed no protective effect on the liver. The treatment with the extract induced an increase of serum aspartate aminotransferase, indicating a rise of toxicity. Contrarily, the same treatment reduced the APAP induced lesion in kidney, with respect to ν-glutamyltransferase. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetaminophen; Melissa officinalis; Pleurisy; Anti-inflammatory; Kidney; Liver.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300006

Purification and properties of asparaginase from the testa of immature seeds of pea (Pisum sativum L.)
Provedor de dados: 52
Autores: Chagas,Eliana P.; Sodek,Ladaslav.
A K+-dependent asparaginase (E.C. 3.5.1.1.) was purified 1328-fold from the testas of immature pea seeds (Pisum sativum L., var. Bolero) and characterized. Antibodies raised against purified asparaginase cross-reacted with the putative asparaginase band in Western blot analyses of semi-purified extracts. However, for crude extracts of pea testas, a cross-reaction was obtained with at least four protein bands, one of which was asparaginase protein. Affinity-purified antibodies to the four strongest bands of crude extracts were fairly specific for the bands from which they were purified, suggesting a mixture of specific antibodies. The Mr of asparaginase was 69,000 by Sephacryl S200 chromatography and also by mobility on native PAGE relative to BSA. There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pisum sativum; Asparaginase; Purification; Properties.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300004

Bioindicators in radiation protection
Provedor de dados: 52
Autores: Amaral,Ademir; Fernandes,Thiago Salazar; Cavalcanti,Mariana Brayner.
Biodosimetry is the evaluation of absorbed dose using bioindicators. Among chromosomal aberrations, scoring of dicentrics from peripheral human blood has been used as gold standard for biodosimetry, although in case of large scale incidents its use presents some drawbacks. Advances in technology have led to new investigations allowing or permitting the use of new methods which not only improve this "classical" biodosimetry but permits the design of other bioindicators making possible faster analyses, particularly in events where many persons may have been exposed. This report presents an overview of some recent studies developed by the "Grupo de Estudos em Radioproteção e Radioecologia - GERAR", Nuclear Energy Department of UFPE - Brazil, involving...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodosimetry; Physical Dosimetry; Radioprotection; Bioindicators; Protein expression.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700015

Seasonal abundance of Henosepilachna vigintioctopunctata (Fab.) on Solanum melongena L. and natural occurrence of its two hymenopteran parasitoids
Provedor de dados: 52
Autores: Jamwal,Vishav Vir Singh; Ahmad,Hafeez; Sharma,Ashutosh; Sharma,Devinder.
ABSTRACT The 28-spotted hadda beetle Henosepilachna vigintioctopunctata Fab. (Coleoptera: Coccinellidae) is a poly-phagous pest, commonly infesting solanacious crops including brinjal, Solanum melongena L. Upon its severe infestation on brinjal, it causes considerable damage to the foliage and also to the calyx of fruits. The studies were made to record the seasonal abundance of hadda beetle and identification of its natural enemies present in the region for two consecutive years. The pooled data for two years showed that the maximum number of H. vigintioctopunctata egg clusters on brinjal were recorded in the 27th (0.40 egg cluster/ plant), followed by grub population in 35th (3.78 grubs/ plant), pupae in 33rd and 39th (0.83 pupae/ plant) and adult...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bio-control; Hadda beetle; Pediobius foveolatus; Seasonal incidence; Tetrastichus sp.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100202

Cells of yeasts adhered in corn grains and the storage perspective for use as probiotic
Provedor de dados: 52
Autores: Baptista,Antonio Sampaio; Horii,Jorge; Piedade,Sonia Maria de Stefano.
Yeasts were applied to corn grains, containing 16 or 20% moisture, at concentrations of 1 and 2%. The cellular viability was assayed at 0, 15, 30, 90 and 110 days. The cellular viability did not differ statistically among the treatments up to 30 days of storage, with the median viability of 89.10%. The average viability found at 90 days (72.20%) was lower than in the first three storage periods. After 110 days, the average viability was 61.14%. In conclusion, since yeast cells were viable up to 110 days in storage on corn grains, these can be used as a vehicle for the application of yeast as a probiotic.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellular viability; Saccharomyces cerevisiae; Storage; Corn grain.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200012

Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres
Provedor de dados: 52
Autores: Chen,Meng-Yan; Zheng,Wei; Dong,Qiu-Yue; Li,Zhen-Hua; Shi,Lu-E; Tang,Zhen-Xing.
In this work, alginate-whey protein was used as wall materials for encapsulating Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The characteristics of encapsulated and free L. bulgaricus showed that the free L. bulgaricus lost viability after 1 min exposure to simulated gastric fluid (SGF) at pH 2.0 and 2.5. However, the viability of encapsulated L. bulgaricus did not decrease in SGF at pH 2.5 for 2 h incubation. The viable numbers of encapsulated L. bulgaricus decreased less than 1.0 log unit for 2 h incubation in SGF at pH 2.0. For bile stability, only 1.2 log units and 2.0 log units viability of the encapsulated L. bulgaricus was lost in 1 and 2% bile for 1 h exposure, respectively, compared with no survival of free L. bulgaricus under the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Encapsulation; Alginate; Whey protein; Probiotics; Stability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500736

Association of plasmid typing to biotyping and antibiotyping in the characterization of outbreaks by Acinetobacter baumannii
Provedor de dados: 52
Autores: Tognim,Maria Cristina Bronharo; Gaziri,Luiz Carlos Jabur; Vidotto,Marilda Carlos; Perugini,Marcia Regina.
During an outbreak at an University Hospital, from April to September, in 1994, sixteen strains of Acinetobacter baumannii were isolated from patients and one strain from an enteral solution. We afterwards analyzed the outbreak by means of plasmid typing, antibiotic resistance typing and biotyping. Two main plasmid profiles were identified. Twelve strains belonged to biotype 2, and five to biotype 19. Susceptibility to amikacin and to carbenicillin allowed classification of the strains into two groups. The results show that association of those three typing methods allowed the differentiation of what was at first considered as a single outbreak into two apparently unrelated outbreaks.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acinetobacter baumannii; Outbreak; Plasmid/biotyping.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100005

Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
Provedor de dados: 52
Autores: Polinarski,Celso Aparecido; Silva,José Luis da Conceição; Mikami,Liya Regina; Fernandez,Maria Aparecida.
A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect polytene cells; Nuclear matrix; Nuclear halo; DNA loops; Salivary gland; Bradysia hygida.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300004

Relations among Valencia orange yields with soil and leaf nutrients in Northwestern Paraná, Brazil
Provedor de dados: 52
Autores: Fidalski,Jonez; Auler,Pedro Antonio Martins; Tormem,Valdomiro.
The Valencia orange orchards established on soils of low fertility in the Northwest region of Paraná State, Brazil, have showed symptoms of Mg deficiency and reduced fruit yields. The objective of this study was to verify the relationship between yield with soil and leaf nutrients during 1996/97 growing season. Two sites of low and high productivity were selected in seven orchards. Leaf and soil samples (fertilized rows and interrows) were collected in 1996. The results showed that the citrus yields were negatively related with soil Mg/K and Ca+Mg/K ratios in the fertilized rows, and fruit weight positively correlated with leaf Zn in the low productivity orchards. The fruit weight was positively related with leaf Ca and soil Ca in the fertilized rows of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Foliar diagnosis; Liming; K fertilization; Calcium; Zinc.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400006

Predacious activity of Ants (Hymenoptera: Formicidae) in conventional and in No-till agriculture systems
Provedor de dados: 52
Autores: Lange,Denise; Fernandes,Wedson Desidério; Raizer,Josué; Faccenda,Odival.
This study had the objective of assessing the differences in foraging activity of the predacious ants between two areas, one conventional and other with no-till agriculture systems. The research was conducted in two contiguous 1.5 ha plots in Dourados MS, Brazil, from February 2001 to December 2003. Each plot received 750 baits (Nasutitermes termites), 425 at daylight and 325 at night. The termites were placed on the filter paper, on the ground, and ant attack was monitored for 15 min, until removed. Sixteen ant species were found in the no-till system and nine in the conventional system. Baits removed from no-till were significantly higher than the conventional plots and were influenced by the sampling time, at day or night. The seasons of the year did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agroecosystems; Biological control; Community structure; Soil tillage; Species richness; Predation effects.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600015

Biological aspects of Dirphia moderata (Lepidoptera: Saturniidae) on Eucalyptus cloeziana and Psidium guajava
Provedor de dados: 52
Autores: Pereira,Fabricio Fagundes; Felipe,João Paulo de Mello; Canevari,Glauco da Cruz; Mielke,Olaf Hermann Hendrik; Zanuncio,José Cola; Serrão,José Eduardo.
An egg mass of Dirphia moderata Bouvier was collected around a branch of Eucalyptus cloeziana to register the occurrence and to study the biological aspects of this species fed with leaves of E. cloeziana or P. guajava at 25 ± 2º C, relative humidity of 60 ± 10% and photo phase of 12 h. The duration and viability of the larva stage of D. moderata was 56.01 days and 80.00% and 55.79 days and 72.5% for its caterpillars fed with the first and second host plants, respectively. The duration (days) and viability (%) of the pupae stage of this insect were 37.64 and 87.00 fed with E. cloeziana and 49.93 and 87.75 with P. guajava, respectively. The average longevity (days) was 6.79 and 8.85 for males and 10.48 and 10.09 for females with E. cloeziana and P. guajava,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus defoliator; Saturniidae; Dirphia.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200017

Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by  Streptomyces lincolnensis Immobilized Cells
Provedor de dados: 52
Autores: Abdelwahed,Nayera A.M.; Gomaa,Eman Zakaria; Hassan,Amira A.
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optimization; Lincomycin; Streptomyces lincolnensis; Response surface methodology; Immobilization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100405

Immature culicidae (Diptera) collected from the Igapó lake located in the urban area of Londrina, Paraná, Brazil
Provedor de dados: 52
Autores: Lopes,José; Zequi,João A. C.; Nunes,Valdecir; Oliveira,Osmar de; O. Neto,Bertolino P. de; Rodrigues,Walter.
The Igapó lake dam is located in an urban area in Londrina, Paraná, Brazil. A one year study of the Culicidae immature forms was conducted in this ecosystem. From a total of 962 collected larvae following genera were identified: Anopheles (59.36%), Culex (19.65%) Aedeomyia (19.23%) Aedes (1.50%), and Uranotaenia (0.10%). The 10 most abundant species were Anopheles strodei, Aedeomyia squamipennis, Culex (Melanoconium) spp., Culex mollis, Anopheles oswaldoi, Anopheles evansae, Culex coronator, Culex quinquefasciatus, Anopheles argyritarsis and Aedes terrens respectively. An. strodei prevailed with greater averages during July and September, showing a significant negative lineal correlation in relation to the rainfall. Ad. squamipennis showed a positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immature Culicidae; Lake; Larvae.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600010

Statistical Optimisation of Fermentation Media for Novel Clostridium novyi -NT Using Response Surface Methodology.
Provedor de dados: 52
Autores: Krishnamoorthy,Kannan; Landge,Anil Vijay.
ABSTRACT Clostridial collagenase is recognized as one of the important proteolytic enzymes used in treatment of varieties of fibro-proliferative disorders. The present work aimed to optimise the biomass concentration and collagenase enzyme activity of novel bacilli Clostridium novyi-NT. The response surface methodology tool was used to identify the optimal fermentation parameters. Central composite design (CCD) was applied with respect to three influencing factors - pH, proteose peptone and trypticase soya broth. These factors showed significant effect on the overall biomass concentration and collagenase enzyme activity (p&lt;0.05). The maximum biomass concentration in terms of absorbance and collagenase activity (of crude enzyme) was achieved and recorded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagenase; Clostridium novyi-NT; Fibro-proliferative disorders; Response Surface Methodology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100429

Use of the arbuscular mycorrhizal fungus Glomus intraradices as biological control agent of the nematode Nacobbus aberrans parasitizing tomato
Provedor de dados: 52
Autores: Marro,Nicolás; Lax,Paola; Cabello,Marta; Doucet,Marcelo Edmundo; Becerra,Alejandra Gabriela.
The plant-parasitic nematode Nacobbus aberrans is an endoparasite that induces gall formation in the roots and causes severe losses to diverse crops. Some populations of this nematode show preference for certain hosts, revealing the existence of "races/groups" with different behaviour and making nematode management difficult. A possible biological control alternative to reduce the damage caused by this species may be the use of arbuscular mycorrhizal fungi (AMF). In the present work, the effect of Glomus intraradices on tomato plants inoculated with the nematode at transplanting and three weeks later was tested. At 60 days, the following parameters were estimated: percentage of AMF colonization, root and aerial dry weight, number of galls and egg masses,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arbuscular mycorrhiza; False root-knot nematode; Microbiological control; Plant protection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500668

Combining Ability for Resistance to White Mold in a Diallel Cross of Soybean
Provedor de dados: 52
Autores: Pereira,Fernanda Aparecida Castro; Vello,Natal Antonio; Rocha,Gabriela Antônia de Freitas; Nekatschalow,Marcos Custódio.
Abstract The white mold, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is one of the most important diseases of soybean. The objective of the present work was to evaluate the soybean reaction to the fungus S. sclerotiorum, which causes white mold, in a partial diallel with 50 crosses. The Group I of parents was composed of ten experimental lines with high grain yield and the group II consisted in five genotypes with possible resistance to white mold. Ten plants of each cross in the F4 generation and the parents were evaluated for reaction to fungus infection using the method of inoculation in detached leaves in order to assess the severity of the disease and to later estimate the combining abilities. Estimates of the specific combining ability (SCA)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Sclerotinia sclerotiorum; Breeding; Phytopathology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100210

Potencial de pseudomonas spp. fluorescentes para biocontrole de alternaria ricini em mamoneira
Provedor de dados: 52
Autores: Silva,Francisco de A.G. da; Peixoto,Cecília do N.; Assis,Sayonara M.P. de; Mariano,Rosa de L.R.; Padovan,Isaíras P.
The potential of fluorescent Pseudomonas spp. to control Alternaria leaf spot on castorbean, caused by Alternaria ricini, was studied under greenhouse conditions. Two periods for antagonist applications were tested: 48h before and simultaneously to the pathogen inoculation. Among the antagonists tested JA4 and BJ22 were the most effectives showing disease severity reduction of 20.9% and 17.8% respectively, when applied simultaneously. The effect of Pseudomonas spp. on the micelial growth and sporulation was also studied throughout three different methods (funel, streak and celophane). Inhibition of micelial growth and sporulation was observed. There was no correlation between in vitro and in vivo data. Antibiosis was showed as a mode of action for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Ricinus communis; Alternaria ricin; Controle biológico.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100010

Healing effect of the ointment made of Equisetum pyramidale in the treatment of cutaneous lesions in diabetic rats
Provedor de dados: 52
Autores: Corrêa,Andréia Cristina Lopes; Hans Filho,Guinter; Dourado,Doroty Mesquita; Matias,Rosemary; Silva,Iandara Schettert da; Sarragiotto,Maria Helena.
This study aimed to evaluate the anti-inflammatory and repair effects of the ethanolic extract and ointment of Equisetum pyramidale on the skin lesions of rats induced to diabetes. After the induction of diabetes with alloxan, a surgical procedure was performed on the back of each rat. Then, they were separated into treatment groups: G1 ethanol extract of E. pyramidale; G2-ointment (extract of E. pyramidale 80g + 20g of vaseline:lanolin 1:1); G3 control (vehicle vaseline:lanolin 1:1); and G4-no treatment during 3, 7 and 14 days. The samples were embedded in paraffin and stained with hematoxylin-eosin for histological analysis. The findings showed that the use of ethanolic extract as well as the ointment decreased the inflammatory cells at the site of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Skin lesions; Medicinal plants; Anti-inflammatory activity; Reepithelialization tissue.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300005

Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
Provedor de dados: 52
Autores: Barreto,George Emílio Sampaio; Oliveira,Elineusa Silva de; Villar,Flávio Augusto Guerreiro Aragão de; Santos,Gleide Souza dos; Lima,Rute Maria Ferreira; Costa,Silvia Lima; Costa,Maria de Fátima Dias; Egito,Eryvaldo Sócrates Tabosa; El-Bachá,Ramon Santos.
The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astrocytes; Brain; Cytotoxicity; 3-methylcatechol; Peroxidation; Respiration.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012

Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
Provedor de dados: 52
Autores: Ferreira,Alessandra Einsfeld; Canal,Natália; Morales,Daiana; Fuentefria,Daiane Bopp; Corção,Gertrudes.
The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121ºC for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU /mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus mundtii; Enterocins; Antimicrobial activity; Biopreservative.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200010

Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
Provedor de dados: 52
Autores: Singh,Jagdish; Verma,Neelam.
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Glucose oxidase; Aqueous two phase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007

Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses
Provedor de dados: 52
Autores: Deef,Lamiaa Elsayed Mokhtar.
ABSTRACT  Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; PCR; Quail; SDS-PAGE; Galliformes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425

A model describing the relationship between electrical conductivity and forage dry matter content
Provedor de dados: 52
Autores: Ashbell,Gilad; Evers,Evert; Bosma,Ate H.
The correlation between dry matter (DM) content, electrical conductivity (EC) and ash content was found to be linear in grass and alfalfa during drying. While minerals in grass and alfalfa were very stable components during drying, their concentrations and EC increase accordingly; therefore, the EC value could serve as a measurement to determine DM content. Results indicated that EC measurement in grass and alfalfa during drying was simple, accurate, fast and easily repeatable, and could serve as an alternative method for DM determination.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrical conductivity; Dry matter; Rye grass; Alfalfa.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400002

Elicitation Enhanced the Production of Phenolic Compounds and Biological Activities in Hairy Root Cultures of Bitter melon ( Momordica charantia L.)
Provedor de dados: 52
Autores: Chung,Ill-Min; Thiruvengadam,Muthu; Rekha,Kaliyaperumal; Rajakumar,Govindasamy.
ABSTRACT Momordica charantia (Cucurbitaceae) is an important vegetable and also medicinal crop which produces the bioactive compounds for various biological activities with potential uses in human health. The present investigation relates to elicitors of jasmonic acid (JA) and salicylic acid (SA) to enhance biomass accumulation and phenolic compound production in hairy root cultures of M. charantia. Hairy root cultures were elicited with JA and SA at 0, 25, 50 and 100 μM concentrations respectively. The adding of elicitation to the hairy root cultures on the 15th day of culture and the roots were harvested on day 25. Cultures supplemented with 100 μM JA and SA enhanced the phenolic compounds significantly compared to that of non-elicited hairy root...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticancer activity; Antioxidant activity; Antimicrobial activity; Elicitation; Hairy root cultures; Phenolic compounds.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100430

Effect of ractopamine on lipid metabolism in vivo - a systematic review
Provedor de dados: 52
Autores: Ferreira,Matheus Soares da Silva; Garbossa,Cesar Augusto Pospissil; Oberlender,Guilherme; Pereira,Luciano José; Zangeronimo,Márcio Gilberto; Sousa,Raimundo Vicente de; Cantarelli,Vinícius de Souza.
The aim of this article was to evaluate the in vivo mechanism of action through which ractopamine reduces the amount of adipose tissue in carcass of animals fed diets supplemented with this drug. Literature search was carried out, using the keywords Ractopamine, lipogenesis, lipolysis, fatty acid and adipose tissue. The scoring system was designed for the adequacy of the methodology. A total of eight papers were found - all published in the English language. The determination of lipolysis and lipogenesis was carried out using different methods - from the quantification of non-esterified fatty acids in the blood to molecular analyses of adipose tissue. The review analysis suggested that, at least in the pigs, the use of this drug reduced lipid deposition in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-adrenergic agonist; Lipogenesis; Lipolysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100005

Green Energy Generation Using FLC Based WECS With Lithium Ion Polymer Batteries
Provedor de dados: 52
Autores: M,Baskar; V,Jamuna.
ABSTRACT Green Energy Generation Using Wind energy conversion system is achieved using Lithium Ion Polymer Batteries and Fuzzy logic controller. Presented scheme also provides the constant output power for the stand alone loads like Island, Hills Stations, Ships and Remote locations etc. A fuzzy-logic controller based Wind energy conversion system with permanent magnet synchronous machine is simulated using MATLAB Simulink. The controller provides the constant output voltage in Buck Boost Converter with the wind fluctuations. The SPWM based inverter can be used to produce the constant output voltage with constant frequency. Also a thin and light weight Lithium Ion Polymer Batteries provides the energy back to the Wind energy conversion system , when the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fuzzy Logic Controller; Sinusoidal pulse width modulation; Wind energy conversion system.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300602

Nematode parasites of Pescada Gó, Macrodon ancylodon Bloch and Schneider, 1801 (Osteichthyes, Sciaenidae), from Vila dos Pescadores, Bragança-PA, Brazil
Provedor de dados: 52
Autores: Fujimoto,Rodrigo Yudi; Sarmento,Aline de Melo Braun; Diniz,Daniel Guerreiro; Eiras,Jorge Costa.
The objective of this work was to evaluate the host/parasite relationship of nematodes in Macrodon ancylodon. Ninety seven fishes (50 in wet season and 47 in dry season) were weighed (167.1 ± 109.9g) and measured (28.3 ± 5.2cm). Only twelve specimens were not infected by any parasites and the prevalence of infection was 87.6%. The highest prevalence values were observed in August and September (dry season, 100%). The stomach was the most infected organ during the whole months (prevalence of 64.2%, and mean intensity of 4.6±7.8 parasites/fish), and the medium intestine showed the lowest infection (prevalence 27.3% and mean intensity 2.5±2.1 parasites/fish). The nematodes were identified as Raphidascaris sp., Goezia sp. and Cucullanus sp. Only the male and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrodon ancylodon; Pescada Gó; Nematodes; Raphidascaris sp; Goezia sp; Cucullanus sp.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600009

Torularhodin and Torulene: Bioproduction, Properties and Prospective Applications in Food and Cosmetics - a Review
Provedor de dados: 52
Autores: Zoz,Liliana; Carvalho,Julio Cesar; Soccol,Vanete Thomaz; Casagrande,Thais Costa; Cardoso,Ligia.
Torularhodin and torulene are two widespread microbial carotenoids with relatively few studies, as compared to other nutraceutical carotenoids such as β-carotene, lycopene and astaxanthin. Several genera of microorganisms produce it in high concentration (up to 0.1% of the cell dry weight), probably as a protection against photooxidation and free radicals. These pigments, which differ by a terminal carboxylic group, have provitamin-A activity and, being red, have potential use as food and cosmetic color additives. Several factors affect the biosynthesis of these substances, including: the composition of culture media, light irradiation, which may enhance the carotenoid production up to 25% of the non-irradiated cultures, and temperature, which changes the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carotenoid; Antioxidant; Microorganism; Biopigment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200278

Diatom assemblage in a tropical lake of northeastern Brazil
Provedor de dados: 52
Autores: Nascimento,Lilian Rodrigues do; Sifeddine,Abdelfettah; Torgan,Lezilda Carvalho; Albuquerque,Ana Luiza Spadano.
The composition and spatial variation of diatom assemblage in surface sediments of Caçó Lake (shallow, mesotrophic and weakly acid lake - Maranhão State, Brazil) were analyzed in order to know the distribution pattern of the species along the lake during rainy season (April 1999). Four zones were established in the lake based on 21 diatoms species and habitat affinities. The first three zones (prime three meters deep to six meters deep) were marked by the occurrence of Pinnularia gigas, Frustulia rhomboides, Encyonopsis krasskei, Eunotia camelus, E. femoriformis and E. monodon. Zone IV (seven to nine meters deep) was inhabited mainly by Surirella biseriata and Fragilariforma floridana. During the beginning of the rainy season, the diatom assemblage in Caçó...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diatoms; Shallow lake; Surface sediments; Caçó Lake; Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100030

Temperature-dependent alterations in metabolic enzymes and proteins of three ecophysiologically different species of earthworms
Provedor de dados: 52
Autores: Tripathi,G; Kachhwaha,N.; Dabi,I.; Bandooni,N.
The effects of varying temperatures (12 - 44° C) on the specific activity of cytoplasmic malate dehydrogenase ((cMDH), mitochondrial malate dehydrogenase (mMDH) and lactate dehydrogenase (LDH) of some earthworms (Metaphire posthuma, Perionyx sansibaricus and Lampito mauritii) were studied. The effects of different temperatures on supernatant and mitochondrial protein contents were also investigated. The specific activities of cMDH, mMDH and LDH of the earthworms decreased gradually as a function of increasing temperature from 12 to 44°C. Higher metabolic energy was needed to maintain the activity at low temperatures. Hence, the earthworms showed increased enzyme specific activity at low temperatures. However, the protein content increased upto 28°C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Proteins; Earthworms; Temperatures.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400017

Design and evaluation of famotidine mucoadhesive nanoparticles for aspirin induced ulcer treatment
Provedor de dados: 52
Autores: Patel,Dhaval J; Patel,Jayvadan K.
The present study was performed to design and evaluate the famotidine loaded mucoadhesive nanosuspension for aspirin induced ulcer. A 3-factor, 3-level Box-Behnken design was applied to study the effects of amount of the beads (X1), PVPK-30(X2) and Tween-80 (X3) on the particle size (Y1), and cumulative percentage drug released after 1h (Y2). The optimization was performed using the desirability function and contour plots. The scanning electron microscopy (SEM) showed the nanoparticles as spherical in shape. The differential scanning calorimetry (DSC) analysis indicated that there was substantial crystallinity change in the nanoparticle compared with the pure drug. Ex-vivo mucoadhesion study showed that famotidine mucoadhesive nanoparticles possessed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Famotidine; Nanosuspension; Box-Behnken design; Mucoadhesion; Peptic ulcer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200007

Environmental sanitation and peri-domiciliar organisation as auxiliary practices for the control of phlebotomines in Paraná state, southern Brazil
Provedor de dados: 52
Autores: Teodoro,Ueslei; Balduíno,João; Thomaz-Soccol,Vanete; Barbosa,Orlando C; Ferreira,Maria E. M. C; Lozovei,Ana Leuch; Verzignassi,Thaís G; Roberto,Andrea C. B. Silva.
Studies were conducted to compare the results of the collections of phlebotomines, in two distinct periods, i.e. before and after changed environmental conditions in Jussara county, Paraná State, Brazil. Collections were made near to a forest in Jussara farm, between February and April 1992. Six Falcão light traps were installed in houses and animals' shelters. Catches were made once a month and 35,783 phlebotomines were captured. Based on these data, sanitation was done in peridomiciliary area and domestic animals shelters were separated from domiciles in January 1994. Then during February to April 1994 using the same methodology new phlebotomines catches were made. The results showed that an average of just 150 (6,311/42) phlebotomines per hour were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Psychodidae; Phlebotomine; Sandflies; Ecology; Lutzomyia.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300007

A simple approach to integrate the ecotoxicological and chemical data for the establishment of environmental risk levels
Provedor de dados: 52
Autores: Cesar,Augusto; Abessa,Denis Moledo de Souza; Pereira,Camilo Dias Seabra; Santos,Aldo Ramos; Fernández,Nuria; Choueri,Rodrigo Brasil; DelValls,Tomas Angel.
In this work, multivariate and numeric methods were used to integrate the chemical and ecotoxicological data obtained for the sediments from the Santos Estuarine System, and for the vicinity of the discharges of the Submarine Sewage Outfall of Santos, in order to establish more accurately the environmental risks, identify the priority areas and thus provide guidance to control the programs and public policies. For both the datasets, the concentrations which exceeded numeric sediment guidelines tended to be associated to toxicity. For the estuary, this trend was corroborated by the correlations between the toxicity and Cu and PAHs levels, whereas for the sewage outfall region, this was observed through the correlation between the toxicity and Hg contents....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sediment; Contamination; Toxicity; Tiburonella viscana; Environmental risk assessment.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100028

Lysine and metabolyzable energy requirements of lactating sows for subsequent reproductive performance
Provedor de dados: 52
Autores: Santos,José Maurício Gonçalves dos; Moreira,Ivan; Martins,Elias Nunes.
The requirements of lactating sows for total lysine and metabolyzable energy (ME) to support subsequent reproduction performance were evaluated. One hundred and twenty sows were used. The treatments were constituted of eight diets (factorial arrangement of four levels of total lysine: 0.75, 0.90, 1.05 and 1.20%, and two levels of ME: 3,250 and 3,400 kcal ME/kg). There was interaction between lysine and ME on the plasma urea nitrogen (PUN), with linear decrease effect on level of 3,250 kcal ME/kg, and quadratic effect on level of 3,400 kcal ME/kg, with the lowest level of PUN with 1.06% lysine. The reproductive performance in the subsequent farrowing was not affected by the lysine levels and ME, hence, neither the total born nor the born alive differed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feed intake; Litter; Plasma urea nitrogen; Weaning-to-estrus interval.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500006

Biopigments from Monascus: strains selection, citrinin production and color stability
Provedor de dados: 52
Autores: Carvalho,Júlio Cesar de; Oishi,Bruno Oliva; Pandey,Ashok; Soccol,Carlos Ricardo.
Fungi form the genus Monascus are a promising source for natural color additives. However, before effectively applying Monascus to foods, it is important to select strains which produce large amounts of biopigments but little or no citrinin, a mycotoxin usually also produced by these fungi. Also, color stability of these pigments should be properly investigated. In order to compare Monascus strains for biopigment production in solid substrate fermentation (SSF), 4 strains (NRRL 1991, NRRL 2897, CCT 3802 and LPB 31) were cultivated over PDA in Petri dishes, and compared for radial growth velocity. Also, these strains were cultivated over cooked rice, and compared in relation to their capacity to produce biopigments and citrinin. The results showed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monascus; Biopigment; Citrinin; Solid-substrate fermentation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800004

Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep
Provedor de dados: 52
Autores: Mondal,Sukanta; Mor,Avantika; Reddy,Ippala Janardana; Nandi,Sumanta; Parameswaragupta,PS.
The present study evaluated the effect of fibroblast growth factor 2 (FGF2) and insulin-transferrin-selenium (ITS) to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets of eight different culture systems: (i) TCM-199 (Tissue Culture Medium-199); (ii) TCM-199+10 ng/mL FGF2; (iii) TCM-199+20 ng/mL FGF2; (iv) TCM-199+30 ng/mL FGF2; (v) TCM-199+10 ng/mL ITS; (vi) TCM-199+20 ng/mL ITS; (vii) TCM-199+30 ng/mL ITS and (viii) TCM-199+20 ng/mL ITS+20 ng/mL FGF2 in a CO2 incubator at 38.50C for 24 h. All the oocyte culture media were supplemented with 10% FBS, FSH (10 μg/mL) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast growth factor 2; Insulin transferrin selenium; Oocyte; Embryo; Sheep.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400521

Erratum: Administration of venlafaxine after chronic methadone detoxification blocks post-depression relapse in rats
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100906

Influence of High Light Intensity and Nitrate Deprivation on Growth and Biochemical Composition of the Marine Microalgae Isochrysis galbana
Provedor de dados: 52
Autores: Mishra,Neha; Prasad,Sheo Mohan; Mishra,Neetu.
Abstract Isochrysis galbana is a brown microalgae widely used as a feed for marine organism in aquaculture. The aim of present study is to investigate the growth, biochemical composition, fatty acid profile, photosynthetic parameters and antioxidant activity (radical scavenging activity) of Isochrysis galbana cells cultivated under different levels (sub-optimum; 50 ± 1.5,optimum; 125 ± 2.5 and supra-optimum; 325 ± 3.5 µmol photons m-2 s -1) of photosynthetic active radiation (PAR), and subsequently treated with different concentration of nitrate deprivation (8mM, 2mM and 0.5mM). The experiment was carried out under controlled conditions utilizing a factorial design 3x3 (light intensity and nitrate concentration). The result depicts that PAR positively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isochrysis galbana Photosynthetically active radiation Nitrate deprivation Biochemical composition Pigments; Antioxidant.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100426

Formas orgânicas e inorgânicas de mobilização do cálcio no solo
Provedor de dados: 52
Autores: Ziglio,Cláudio M.; Miyazawa,Mario; Pavan,Marcos A.
A falta de Ca e a presença de Al na subsuperfície do solo restringem o crescimento das raízes. A eficiência da calagem aplicada na superfície do solo na neutralização da acidez subsuperficial é limitada. Foram conduzidos experimentos de laboratório com colunas de PVC para avaliar técnicas orgânicas e inorgânicas na mobilidade do Ca em solo com carga variável. Foram conduzidos dois experimentos com sais de Ca: CaCO3, CaSO4, Ca(NO3)2, Ca-acetato e Ca-citrato, e outro com CaCO3 associado com resíduos de aveia preta, trigo e leucena. Avaliaram-se os teores de Ca trocável em solucão de KCl 1M nas profundidades de 0 a 5, 5 a 10, 10 a 20, 20 a 30, 30 a 40 e 40 a 50cm. A eficiência dos sais na mobilidade de Ca seguiu a ordem: NO3 &gt; Acetado &gt; Citrato &gt; SO4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca-leaching; Subsoil acidity; Green manure; Plant residue.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200016

Evaluation of Genetic Analysis of ESCHERICHIA COLI Isolated from Two Different Environmental Sources: Sewage Water Verses Soiled Bedding Materials of Laboratory Rodents
Provedor de dados: 52
Autores: Kumar,Sharad; Kumar,Mahadeo; Raj,Abhay; Prakash,Jyoti.
ABSTRACT The present investigation details an assessment of genetic relationship of E. coli isolates collected from two different environmental sources viz. sewage water and soiled bedding materials of laboratory rodents. Five sewage water samples were collected from the industrial area of Lucknow city and 5 samples of soiled bedding materials of laboratory animals were collected from Animal facility at CSIR-IITR, Lucknow. For this study Random amplified polymorphic DNA markers (RAPD) was chosen as the molecular fingerprinting method. In this study, 10 RAPD primers were used to evaluate the genetic similarity of E. coli. isolates. The RAPD-PCR fingerprints were analyzed and data were scored as 1, 0 matrix. The generated data were fed on Popgene software...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Genetic similarity; RAPD-PCR and Rodents.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100445

Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
Provedor de dados: 52
Autores: Esmaelizad,Majid; Niaraki,Saber Jelokhani; Fesharaki,Reza Hashemi.
The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tams1; Theileria Annulata; Merozoite surface antigen; Phylogenetic.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005

Signatures of Selection From Candidate Gene Polymorphisms Panel in Five Zebu Breeds
Provedor de dados: 52
Autores: Parra-Bracamonte,Gaspar Manuel; Martinez-Gonzalez,Juan Carlos; Sifuentes-Rincón,Ana María; Rosa-Reyna,Xochitl Fabiola la.
ABSTRACT The signals of selection using candidate genes polymorphism were studied in five zebu breeds of Mexico. Three loci from GHRH and complementarily Steroyl Co Desaturase F762, Dopamine Beta Hydroxilase 17299, and LEP3272 were identified under selection. Findings depict Zebu selection pressure mainly on Brahman breed resulting in a divergent structure pattern.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Population structure; PCA; Selection signals.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100442

Odanacatib Inhibits Resistin-induced Hypertrophic H9c2 Cardiomyoblast Cells Through LKB1/AMPK Pathway
Provedor de dados: 52
Autores: Zheng,Xian; Liu,Huzi; Cheng,Guanchang; Luo,Jianwei; Ye,Qunhui; Deng,Yongzhi; Wu,Lin.
ABSTRACT Odanacatib (ODN) is a selective inhibitor of cathepsin K. The cysteine protease cathepsin K has been implicated in cardiac hypertrophy. Resistine is an adipokine which is identified to promote cardiac hypertrophy. Here, we hypothesize that ODN mitigates resistin-induced myocyte hypertrophy. Cell surface area and protein synthesis were measured after treatment with resistin and ODN in H9c2 cells. The expression of cardiomyocyte hypertrophy marker BNP and β-MHC was detected by RT-qPCR. The expression and phosphorylation of AMPK and LKB1 were analyzed with Western blot. Resistin could significantly increase cardiomyocyte cell surface area, protein synthesis, and embryonic gene BNP and β-MHC expression, inhibit phosphorylation of AMPK and LKB1. ODN...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resistin; Odanacatib; LKB1/AMPK pathway; Cardiomyocyte hypertrophy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100309

Gene Expression Analyses of HER-2/neu and ESR1 in Patients with Breast Cancer
Provedor de dados: 52
Autores: Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza.
ABSTRACT  Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features.  Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software.  Results: HER2 and ESR1 overexpression was identified in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100304

Biomonitoring of microcystin and aflatoxin co-occurrence in aquaculture using immunohistochemistry and genotoxicity assays
Provedor de dados: 52
Autores: Hashimoto,Elisabete Hiromi; Kamogae,Márcia; Vanzella,Tatiana Perez; Cólus,Ilce Mara Syllus; Bracarense,Ana Paula Frederico Rodrigues Lourenço; Bittencourt-Oliveira,Maria do Carmo; Itano,Eiko; Kuroda,Emília Kiyomi; Kato,Hajime; Nagata,Satoshi; Ueno,Yoshio; Harada,Ken-Ichi; Hirooka,Elisa Yoko.
This work investigated the effects of co-occurring aflatoxin B1 (AFB1) and microcystin (MC) in aquaculture, using immunohistochemistry and genotoxicity methods. Tilapia (Oreochromis niloticus) were exposed to AFB1 by intraperitoneal and MC (cell extract of Microcystis aeruginosa) by intraperitoneal and immersion routes. The interaction of MC-AFB1 was evaluated co-exposing the intraperitoneal doses. Blood samples were collected after 8, 24, and 48h to analyze the micronucleus frequency and comet score. The interaction of MC-AFB1 showed a synergic mutagenic response by higher micronucleus frequency of co-exposed group. A slight genotoxic synergism was also observed in the comet score. Immunohistochemistry detected MC in al lthe fish liver tissues exposed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystis aeruginosa; Mycotoxin and tilapia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100019

Invertebrates associated with Paspalum repens (Poaceae) at the mouth of Caracu Stream (1991-1992), affluent of the Paraná River, Porto Rico - PR - Brazil
Provedor de dados: 52
Autores: Souza-Franco,Gilza Maria de; Takeda,Alice Michiyo.
Temporal distributions of Paspalum repens at the mouth of the Caracu Stream on the left margin of the Paraná River were characterized and analyzed. Samples of Paspalum repens were taken monthly from March 1991 to February 1992. Fauna consisted of many taxa: Gastropoda, Oligochaeta, Copepoda, Ephemeroptera, Hemiptera, Trichoptera, Lepidoptera, Chironomidae, Coleoptera and Hydracarina. With the exception of May 1991, low density of organisms was registered during the sampling period. During May 1991, a sharp decline in the Paraná River water level was noted. This fact suggested that organisms dispersed in the submersed vegetation during the flooding of the Paraná River must have concentrated in the stream margin and increased the density of organisms at this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stream; Fauna; Paspalum repens; Paraná River; Brazil.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300012

Dolomite lime’s reaction applied on the surface of a sandy soil of the Northwest Paraná, Brazil
Provedor de dados: 52
Autores: Meda,Anderson R.; Pavan,Marcos A.; Cassiolato,Marcelo E.; Miyazawa,Mário.
Low Ca and Mg are serious limitations to crop production in sandy soils of the northwest Paraná, Brazil. Thus soil samples of an Oxisol collected in this region were packed into 30cm long columns. Dolomite lime (2.0, 0.84, 0.30, and &lt; 0.30 mm screen) was added on soil surface, then leached with deionized water. Thereafter, the columns were dismantled and the soil cut into 5cm segments for chemical analysis. Dolomite lime increased pHCaCl2,, KCl-exchangeable Ca and Mg and residual CO3 mostly in the top surface layers. Surface dolomite lime had no effect on pH, Ca, Mg, and CO3 in the leachate, independent on the lime particle size. These results indicated that surface dolomite lime application had no effect on subsoil composition and mostly of the calcium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liming; Ca and Mg leaching; Subsoil acidity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200014

Efficiency of n-octyl-acetate, 2-heptanone and citronellal in repelling bees from basil (Ocimum sellowii - Labiatae)
Provedor de dados: 52
Autores: Malerbo-Souza,Darclet Teresinha; Nogueira-Couto,Regina Helena.
The objective of this study was to test repellent substances for honey bees, Apis mellifera, for basil, Ocimum sellowii, and to study the most frequent insects in this crop as well as its behaviors. The frequency of honey bees did not decrease with the application of n.octyl.acetate, citronellal and 2.heptanone, used as repellents, in the concentrations 2.5, 5.0, 7.5 and 10.0% diluted in water. However, when the products (20%) were diluted in water (75%) and glycerine (5%) were effective in repelling those bees of the basil flowers for a period of 20 minutes. The flower lasted, on average, 30 h from the bud phase until the flower withered. The flowers number opened by inflorescence was, on average, 5.65 flowers, and the inflorescence presented, on average,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Repellent; Apis mellifera; Basil; Ocimum sellowii; Pollination; Insects.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100016

Potency Evaluation of Recombinant Human Erythropoietin in Brazil: Assessment of Reproducibility Using a Practical Approach
Provedor de dados: 52
Autores: Nascimento,Michele Cardoso do; Abreu,Clarice Lima do Canto; Costa,Rodrigo Netto; Delgado,Wlamir Correa de Moura; Fernandes,Isabella.
In this study, we compared the results of potency determination of recombinant human erythropoietin (rhEPO) obtained between 2010 and 2012 by the National Institute of Quality Control in Health (INCQS/Fiocruz), i.e., the National Control Laboratory (NCL), and by a manufacturer of rhEPO. In total, 47 different batches of commercially prepared rhEPO (alpha isoform) were analyzed. All results, including those of the control and warning limits, remained within the limits recommended by European Pharmacopoeia (Ph. Eur.). All relative error (RE) values were less than ± 30%, wh ereas most were approximately ± 20%. Applying the Bland-Altman plot, only two of 47 values remained outside the limits of agreement (LA). In addition, agreement of potency determination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant Human Erythropoietin; Biological; Quality Control; Sanitary Surveillance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400562

Bioefficacy of flindersine against Helicoverpa armigera Hübner, Spodoptera litura Fabricius, Anopheles stephensis Liston. and Culex quinquefasciatus Say.
Provedor de dados: 52
Autores: Duraipandiyan,Veeramuthu; Baskar,Kathirvelu; Muthu,Chelliah; Ignacimuthu,Savarimuthu; Al-Dhabi,Naif Abdullah.
Flindersine, an alkaloid isolated from Toddalia asiatica, was evaluated for their antifeedant, larvicidal and growth inhibitory activities against Helicoverpa armigera, Spodoptera litura and larvicidal activity against vector mosquitoes Anopheles stephensi and Culex quinquefasciatus. For this, leaf disc no choice method was used for agricultural pests; larvicidal activity was tested on second and fourth instar larvae for mosquitoes at different concentrations. Flindersine showed antifeedant, larvicidal and growth inhibitory activities against H. armigera and S. litura and larvicidal activity against vector mosquitoes An. stephensi and Cx. quinquefasciatus. It showed high regression (R2) values of 0.91 and 0.87 against H. armigera and S. litura,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flindersine; Adult emergence; Antifeedant; Growth inhibitiory; Larvicidal.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400595

Editorial
Provedor de dados: 52
Autores: Ashraf,Muhammad Aqeel.
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200100

Quantitative analysis of foraging habitat use by ciconiiformes in the upper Paraná river Floodplain, Brazil
Provedor de dados: 52
Autores: Gimenes,Márcio Rodrigo; Anjos,Luiz dos.
The aim of this study was to analyze the seasonal variations in habitat selection and abundance of Ciconiiformes species in four foraging habitats (rivers, channels, connected and disconnected lagoons) on the upper Paraná River floodplain, Brazil, and to conduct the surveys of wading birds in 2002 and 2003. The largest number of species and highest abundances of most species were observed in the connected lagoons. Snowy Egrets (Egretta thula), Wood Storks (Mycteria americana), Roseate Spoonbills (Platalea ajaja), and Jabirus (Jabiru mycteria) frequently used connected lagoons (habitat with the highest fish abundance) and abandoned the areas during floods, suggesting that they were able to find high quality patches for foraging. Cocoi Herons (Ardea cocoi),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wading birds; Ciconiiformes; Prey availability; Floodplain; Paraná river.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200025

Diet of the clupeid fish Platanichthys platana (Regan, 1917) in two different Brazilian coastal lagoons
Provedor de dados: 52
Autores: Aguiaro,Talita; Castelo Branco,Christina Wyss; Verani,José Roberto; Caramaschi,Érica Pellegrini.
Platanichthys platana is considered a constant species in both Cabiúnas and Imboassica lagoons that are characterised by different marine and freshwater inputs, and anthropogenic influences. The stomach content analysis of P. platana captured between July 1991 and July 1993 revealed filamentous algae, detritus, eggs of benthic invertebrates, larvae of chironomids and bivalves as the main food sources in Imboassica lagoon. Small-sized cladocerans, copepods and shrimp larvae were the prevailing items in Cabiúnas lagoon. Seasonal food variations were noted for the fishes of Imboassica lagoon. Diet differences were highlighted within specimens lesser than 40 mm standard length in Imboassica lagoon, and were related to the increase of marine influence due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Coastal lagoons; Food items; Platanichthys platana.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200013

Use of labelled cisplatin obtained by direct irradiation in a preliminary biodistribution study
Provedor de dados: 52
Autores: Carvalho Júnior,Álvaro Dutra de; Abrantes,Fabiana Moreira; Menezes,Maria Ângela; Leal,Alexandre Soares; Cardoso,Valbert Nascimento; Oliveira,Mônica Cristina de.
This work presents the preparation of radiolabelled cis-dichlorodiammineplatinum (II), CDDP*, from its direct irradiation into a cadmium capsule, using the TRIGA MARK I IPR R-1 research reactor of the CDTN. The ability to detect CDDP* in Ehrlich tumour-bearing mice after administration via an intravenous route was evaluated. After 24 hours, blood and some organs were collected to determine the incorporated activity. The CDDP* showed a great chemical purity and high specific activity that resulted in an optimum in vivo detection. The CDDP* was taken up principally by liver, spleen and kidney. The CDDP* obtained from this condition was shown to be a good tool for biodistribution studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiolabelled cisplatin; Biodistribution; TRIGA research reactor.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700013

Patent application in biotechnology at subclass C12N in Brazil at the period of 2001 to 2005
Provedor de dados: 52
Autores: Buttow,Mônica Elisa; Steindel,Mário.
An analysis of Instituto Nacional de Propriedade Industrial (INPI) patent database from 2001 to 2005 showed that less than 5% of the total patent deposited in the subclass C12N, which referred to "Microorganisms or Enzymes, Compositions thereof, Propagating, Preserving or Maintaining Microorganisms, Mutation or Genetic Engineering, Culture Media", were from national depositors. In contrast, more than 34% of all these deposits came from the United States of America during the same period. Among the national depositors, public universities and government research institutions contributed with 83% over all the deposits, demonstrating that research and development in the field of Biotechnology in Brazil has been concentrated within Brazilian public...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Patents; Biotechnology; Intellectual Property; Technologic Innovation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300003

Spirulina as a protein source in the nutritional recovery of Wistar rats
Provedor de dados: 52
Autores: Moreira,Lidiane Muniz; Behling,Bruna del Sacramento; Rodrigues,Rosane da Silva; Costa,Jorge Alberto Vieira; Soares,Leonor Almeida de Souza.
This study was aimed at evaluating the effects of Spirulina (Arthrospira) as a protein source in the nutritional recovery of rats subjected to protein malnutrition. In order to understand such effects, responses of the developing body, serum biochemical profile, and blood count of animals were analyzed. A total of, 23 Wistar Rattus norvegicus were given a proteic diet (AIN-93G adapted) in malnutrition phase (phase I) for 10 days. After this period, the rats were separated into four groups according to the protein source (recovery phase): Control (C), casein 12%; Spirulina level I (S1), Spirulina 8.8% + casein 5.0%; Spirulina level II (S2), Spirulina 17.6% + casein 0.15%, and Spirulina level III (S3), Spirulina 26.4%. Recovery phase (phase II) lasted 30...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein malnutrition; Rats; Nutritional recovery; Rattus norvegicus; Spirulina.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300013

The Effect of Mg2+, Cu2+ and Zn2+ pre-treatment on the color of yerba maté (Ilex paraguariensis) leaves
Provedor de dados: 52
Autores: Scipioni,Griselda Patricia; Argüello,Beatriz del Valle; Schmalko,Miguel Eduardo.
The aim of this work was to study the effect of alkaline blanching followed by an immersion in salt solutions of Mg2+, Cu2+ and Zn2+ on color preservation of Yerba Maté leaves. The concentration of NaOH in the alkaline solution influenced all of the color parameters. Ion concentration and dipped time influenced only some color parameters. The color parameters of the product obtained with different treatments were different from the control sample (blanched with boiling water instead of alkaline blanching) and from the product obtained via industrial processing. The green color in the pre-treated and control samples was more intense (greater values of -a) and darker (low values of L and b). Ionic and ash content in the leaves increased with the treatments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Color; Yerba maté; Ionic treatment; Drying.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600027

Antagonistic activity of antibiotic producing Streptomyces sp. against fish and human pathogenic bacteria
Provedor de dados: 52
Autores: Hossain,Nazmul; Rahman,Mahbubur.
In this study, attempts were made to isolate Streptomyces sp. from soil samples of two different regions of Bangladesh and evaluate their antagonistic activity against fish and human pathogenic bacteria. A total of 10 isolates were identified as Streptomyces sp. based on several morphological, physiological and biochemical tests. Cross streak method was used to observe the antagonistic activity of the Streptomyces sp. isolates against different fish pathogens belonging to the genus Aeromonas, Pseudomonas and Edwardsiella and human clinical isolates belonging to the genus Klebsiella, Salmonella and Streptococcus. Seven Streptomyces sp. isolates showed antagonism against both fish and human pathogenic bacteria. Four isolates viz., N24, N26, N28 and N47...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomyces sp.; Antagonistic activity; Cross streak method; Antibiotic.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200011

Proposal of methodology and test protocol for evaluating and qualifying pH measuring devices
Provedor de dados: 52
Autores: Almeida,Niza Helena de; Nohama,Percy.
We present a proposal for evaluating and qualifying pH measuring devices based on the requirements of relevant standards. The proposal presented is based on ASTM E70, NBR 7353, JIS Z 8805, BS 3145, DIN 19268, NBR ISO 17025 and other standards, as well as the results of field research carried out in conjunction with professionals performing pH measurements in public health laboratories. Evaluation is performed by inspection of a form which records data from the measuring system. The form gives acceptable variations in the parameters being tested and allows a conclusion to be reached regarding acceptability of the system. Using the proposed protocol allows definition of suitable analysis criteria, while taking into account the influence pH measurement is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality control; PH measurement; Instrumentation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200005

Sequence and analysis of the mitochondrial DNA control region in the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)
Provedor de dados: 52
Autores: Bravo,Juliana Pereira; Felipes,Joice; Zanatta,Daniela Bertolini; Silva,José Luis da Conceição; Fernandez,Maria Aparecida.
This study aimed at the sequence and analysis of the mtDNA control region (CR) of the Diatraea saccharalis. The genome PCR amplification was performed using the complementary primers to the flanking regions of Bombyx mori CR mitochondrial segment. The sequencing revealed that the amplified product was 568 bp long, which was smaller than that observed for B. mori (725 bp). Within the amplified segment, a sequence with 338 nucleotides was identified as the control region, which displayed a high AT content (93.5%). The D. saccharalis mtDNA CR multiple sequence alignment analysis showed that this region had high similarity with the Lepidoptera Cydia pomonella.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA Control Region; Diatraea saccharalis; Lepidoptera; Sugarcane borer.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400004

Determination of the Ideal Time for Cleaning of Photovoltaic Panels Aiming at Better Productivity - Case Study of the Implanted Grid-Connected Photovoltaic System at the Federal University of Technology of Paraná
Provedor de dados: 52
Autores: Gaio,João Nicolau; Campos,Kleverson Moisés Apolinário de; Tiepolo,Gerson Máximo.
ABSTRACT Considering the development of new technologies in the electricity generation sector, such as solar photovoltaic energy, some studies are made necessary to obtain the best performance of these systems. This generation model consists of modules, which are exposed to adverse climatic conditions, they receive direct influence of the dirt, according to the environment from which they were installed, which directly influences the performance of the system since, due to the disposal of waste on the module, a smaller portion of the solar radiation can reach the photovoltaic cell effectively. The objective of this work is to determine the ideal time for cleaning photovoltaic panels, aiming at high system productivity, having as object of study the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photovoltaic Systems; Photovoltaic Solar Energy; Dirtiness; Performance Parameters.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200207

Influence of cultivar and rootstock on early growth and syllepsis in nursery trees of pear (Pyrus communis L., Rosaceae)
Provedor de dados: 52
Autores: Milošević,Tomo; Milošević,Nebojša.
A two-year field study was conducted to examine the capacity of spontaneous formation of long sylleptic shoots (LSS) in nursery trees of pear cvs. 'Abbé Fétel', 'Conference' and 'Starking Delicious' grafted on Quince MA (MA) and Quince BA 29 (BA 29) rootstocks in a nursery during the first year after bud grafting. Tree height (TH), trunk diameter (TD) - 10 cm above the bud union and number of LSS were measured at the end of each season. The TH was measured from the ground level. The highest number of LSS was developed by cv. 'Abbé Fétel' in both the seasons, and the lowest by cv. 'Starking Delicious'. Tree height and TD were highly significantly affected by the cultivar in both the years and by the rootstock in 2008. The interactions between them did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Branching; Cultivar; Long sylleptic shoot; Nursery tree; Pyrus communis L.; Rootstock.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300004

Evaluation of antagonistic activity of milk fermented with kefir grains of different origins
Provedor de dados: 52
Autores: Santos,João Paulo Victorino; Araújo,Tatiane Ferreira; Ferreira,Célia Lúcia de Luces Fortes; Goulart,Simone Machado.
In this study, the potential of kefir grains, obtained from three different towns/cities in the Southeast Region of Brazil to inhibit the growth of the five pathogenic microorganisms was evaluated. The samples showed the growth inhibition from 42.80-69.15 for Staphylococcus aureus ATCC 6538, 30.73-59.89 for Escherichia coli ATCC 11229, 44.99-73.05 for Salmonela typhi ATCC 6539, 41.45-54.18 for Listeria monocytogenes ATCC 15313 and 70.38-86.80 for Bacillus cereus RIBO 1222-173-S4. These results indicated that the kefir grains evaluated had antagonistic activity toward the different pathogens tested. The ability to inhibit, although differently depending on the regional microbiota, indicated a potential for their use as a functional food.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kefir; Antagonism; Inhibitors; Functional foods.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500014

Threat of an influenza panzooty: a review based on conservation medicine
Provedor de dados: 52
Autores: Caron,Luiz Felipe; Soccol,Vanete Thomaz.
Among reemerging illnesses, influenza constitutes one of the main concerns. The avian influenza has recently demonstrated the strong transmission capacity of the etiological agent -a virus from the Orthomyxoviridae family - associated to high pathogenic manifestations of the illness. The strong mutation capacity of this virus, through different hosts, reveals how important integrated actions aiming at monitoring its presence in different species are. The swine infection represents an additional concern not only in relation to that species but also in relation to the possibility of the virus to mutate and adapt to humans. The elements that determine the pathogenicity of the various viral subtypes must be well understood, for the tools used to control the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine flu; Avian flu; Reassortment; Conservation medicine; Panzooty.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400010

Nickel adsorption by variable charge soils: effect of pH and ionic strength
Provedor de dados: 52
Autores: Soares,Marcio Roberto; Casagrande,José Carlos; Mouta,Ernesto Rinaldi.
The effects of pH and ionic strength (I) on Ni adsorption in variable charge soils were evaluated by laboratory batch experiments. Experimental results fitted the Langmuir model. Maximum adsorption (Ads max) ranged from 260-2818 mg kg-1 (topsoil) to 532-1541 mg kg-1 (subsoil). Nickel affinity (K L) was higher in the subsoil samples (0.022-0.236 L kg-1) than in topsoil (0.003-0.049 L kg-1). Adsorption envelopes showed sharp increase in Ni adsorption (20-90%) in the 4.0-6.0 pH range. Nickel adsorption was affected by I and specific adsorption predominated in the 3.0-5.0 pH range, while above pH 5.0, it was adsorbed by electrostatic mechanisms. Negative values of free energy variation (ΔG) and the separation factor K R&lt;1 indicated that Ni adsorption...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adsorption isotherms; Free energy; Adsorption envelopes; Adsorption edges; Ionic strength.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100025

Testicular Histomorphometric Evaluation of Zebu Bull Breeds
Provedor de dados: 52
Autores: Andreussi,Paulo Antônio Terrabuio; Costa,Deiler Sampaio; Faria,Fábio José Carvalho; Fernandes,Carlos Antônio Carvalho; Santos,Marcelo Diniz; Silva,Juliana Correa Borges.
The objective of this study was to evaluate the quantitative histology and testicular biometrics in zebu bulls of different breeds. Testicular fragments of Nelore (n=10), Polled Nelore (n=6), Gir (n=5), Guzerat (n=5) and Tabapuã bulls (n=5) were used. The fragments were perfusion-fixed in Karnovsky solution, embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate. The Nelore animals had a higher tubular volumetric proportion (85.2%) and greater height of the seminiferous epithelium (73.2 µm) than the Gir, Guzerat and Tabapuã breeds. The Nelore animals also had a higher volumetric proportion of Leydig cells (5.2%) than the Guzerat and Tabapuã breeds. There was no significant difference for any of these parameters between the Nelore...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Testis; Volumetric proportion; Seminiferous tubule.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600900

Mechanical and permeability properties of milk protein films
Provedor de dados: 52
Autores: Folegatti,Marília I.S.; Antunes,Aloísio José; Marcondes,Jorge A.
Edible films present a potential alternative for replacing plastic films used for packaging in food industry. One of the major advantages is the environmental appeal of this technology, which produces no packaging waste. Some films made with other edible materials have found commercial applications, and many more are being developed using a myriad of food based components. This paper focuses on some important characteristics of films produced with sodium and calcium caseinates. The effects of caseinate type and concentration, plasticizer concentration and pH were studied. Major parameters investigated were solubility, tensile properties, water vapour and oxygen permeabilities. Caseinate films showed high solubility at pH range 6.0-8.0 and complete...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edible films; Sodium caseinate films; Calcium caseinate films.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300008

Predation rate of Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae with and without defense by Podisus nigrispinus (Heteroptera: Pentatomidae)
Provedor de dados: 52
Autores: Zanuncio,José Cola; Silva,Carlos Alberto Domingues da; Lima,Eraldo Rodrigues de; Pereira,Fabrício Fagundes; Ramalho,Francisco de Souza; Serrão,José Eduardo.
The effect of the defense of the prey Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) on predation rates of Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) females was studied. P. nigrispinus preyed 1.0, 1.4, 1.2, 3.8 and 3.0 and 0.4, 0.8, 1.6, 2.8 and 3.2 third instars of S. frugiperda larvae with and without defense, respectively, with the densities of one, two, four, six and eight larvae. The attack rate and the manipulation time were, respectively, 0.67 ± 0.39-h and 6.72 ± 2.88 h for P. nigrispinus females fed with larvae with defense and 2.51 ± 0.16-h and 0.51 ± 0.77 h for those without defense. The functional response of the predator P. nigrispinus varies with the defense and the density of the prey S. frugiperda, with larger...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asopinae; Biological control; Predator; Functional response.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100015

Imaging and therapy with radionuclide labeled magnetic nanoparticles
Provedor de dados: 52
Autores: Ünak,Perihan.
Magnetic nanoparticles offer exciting new opportunities including the improvement of the quality of magnetic resonance imaging (MRI), hyperthermic treatment for malignant cells, site-specific drug delivery and also the recent research interest of manipulating cell membranes. The biological applications of these nanomaterials require these nanoparticles to have high magnetization values, size smaller than 20 nm, narrow particle size distribution and a special surface coating for both avoiding toxicity and allowing the coupling of biomolecules. In this review, we focus on the feasibility of radionuclide labeled magnetic nanoparticles, as drug carriers, and summarize recent advances in this field.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetite Nanoparticles; Radionuclide; Imaging; Therapy; Targeted drug delivery; Iron oxide.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700006

Gamma-ray spectrometry sensor and geochemical prospecting in an area of sugar cane plantation
Provedor de dados: 52
Autores: Becegato,Valter Antonio; Ferreira,Francisco José Fonseca; Cabral,João Batista Pereira; Rafaelli Neto,Sílvio Luis.
In this study the K, U and Th radionuclide concentrations were determined in a sugar cane plantation that received large quantities of fertilizer and gypsum. Geochemical data were obtained for the clay, iron and organic matter in the soil samples, taken from depths of 0-20 and 20-40 cm. The average radionuclide concentrations were 71 Bq Kg-1 for K; 5.97 Bq Kg-1 for U, and 11.26 Bq Kg-1 for Th, and the uranium was variable (56%). The radionuclide average concentrations for the different fertilizer compositions varied in the range of 603.5 - 8855.0 Bq Kg-1 for K; 27.3 - 684.4 Bq Kg-1 for U, and 58 - 788.5 Bq Kg-1 for Th, and it was most variable for K (53%). Gypsum average concentrations were 82.6 Bq Kg-1 for K, 641.5 Bq Kg-1 for U, and 201.7 Bq Kg-1 for Th,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fertilizers; Soils; Radionuclides; Gamma-ray spectrometry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100001

EI Scale: an environmental impact assessment scale related to the construction materials used in the reinforced concrete
Provedor de dados: 52
Autores: Morales,Gilson; Jungles,Antonio Edésio; Klein,Sheila Elisa Scheidemantel; Guarda,Juliana.
This study aimed to create EI Scal, an environmental impact assessment scal, related to construction materials used in the reinforced concrete structure production. The main reason for that was based on the need to classify the environmental impact levels through indicators to assess the damage level process. The scale allowed converting information to estimate the environmental impact caused. Indicators were defined trough the requirements and classification criteria of impact aspects considering the eco-design theory. Moreover, the scale allowed classifying the materials and processes environmental impact through four score categories which resulted in a single final impact score. It was concluded that the EI scale could be cheap, accessible, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental impact; Eco-design; Reinforced concrete production; Construction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600029

Partial characterization of an inulinase produced by Aspergillus japonicus URM5633
Provedor de dados: 52
Autores: Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Porto,Tatiana Souza; Spier,Michele Rigon; Soccol,Carlos Ricardo; Porto,Ana Lúcia Figueiredo; Souza-Motta,Cristina Maria; Moreira,Keila Aparecida.
Enzymes obtained by fermentation processes offer a number of advantages and have been widely researched and used throughout the world. This study aimed to partially characterise an inulinase produced from palm and cassava peel. The enzyme was produced via the solid-state fermentation of Aspergillus japonicus URM5633. The optimal temperatures were 50ºC and 55ºC, and the optimal pH values were 5.2 and 3.4 for inulinase fermentatively produced from palm and cassava peel, respectively. The thermostability measurements for inulinase produced in palm showed that the relative activity remained below 100% until 30 minutes of stability for all temperatures, but reached 106.8% at a temperature of 50ºC after 60 minutes. Inulinase from the crude extract of cassava...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulinase; Characterisation; Solid-state fermentation; Aspergillus japonicus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500005

Evaluation of Oxidative Stability of Compound Oils under Accelerated Storage Conditions
Provedor de dados: 52
Autores: Pazzoti,Geisa; Souza,Camila; Veronezi,Carolina; Luzia,Débora; Jorge,Neuza.
ABSTRACT The oxidative stability of linseed (L), cotton (A), and coconut (C) oils, as well as of linseed:cotton (LA), linseed:coconut (LC), and linseed:cotton:coconut (LAC) compound oils was evaluated under accelerated storage at 60°C/20 days. Coconut oil showed to be rather stable, mainly due to low levels of peroxides, conjugated dienes, ρ-anisidine, and long induction period. In addition, along with cotton oil, it improved the stability of linseed oil in the formulation of LAC compound oil. As to fatty acid profile, the compound oils showed to be composed mainly by unsaturated fatty acids. Cotton and coconut oils presented higher retention of total phytosterols, 78.87 and 76.16%, respectively, after 20 days of storage, when compared to linseed oil. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linseed; Cotton; Coconut; Lipid oxidation; Vegetable oils.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100502

Bioprospection of yeasts as biocontrol agents against phytopathogenic molds
Provedor de dados: 52
Autores: Rosa-Magri,Márcia Maria; Tauk-Tornisielo,Sâmia Maria; Ceccato-Antonini,Sandra Regina.
Yeasts isolated from sugar cane and maize rhizosphere, leaves and stalks were screened against the phytopathogenic molds Colletotrichum sublineolum and Colletotrichum graminicola, both causal agents of the anthracnose disease in sorghum and maize, respectively. Strains identified as Torulaspora globosa and Candida intermedia were able to inhibit the mold growth, with the first species also exhibiting killer activity. No previous report on the application and potentiality of these yeasts as biocontrol agents were found neither the killer phenotype in Torulaspora globosa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioprospection; Colletotrichum graminicola; Colletotrichum sublineolum; Killer activity; Mycocinogenic.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100001

Relationship between the mass of organic matter and carbon in soil
Provedor de dados: 52
Autores: Bianchi,Silmara R.; Miyazawa,Mario; Oliveira,Edson L. de; Pavan,Marcos Antonio.
The quantity of soil organic matter (SOM) was estimated through the determination of soil organic carbon (SOC) times a factor, which assumes that 58% of the SOM was formed by carbon. A number of soil samples with wide range of SOC content collected in the state of Paraná, Brazil were evaluated in the laboratory. SOC was measured by Walkley-Black method and the total SOM by loss on ignition. The SOC was positively correlated with SOM. The SOM/SOC ratio varied from 1.91 to 5.08 for the soils. It shows that Brazilian SOM has greater oxidation degree. Although, the SOM and SOC decreased with soil depth the SOM/SOC ratio increased. It showed that SOM in the subsoil contained more oxygen but less carbon than the SOM in the upper soil surface. The CEC/SOC also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic carbon; Tropical soils; Soil analysis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200005

Enterococcus spp. Resistant to Multiple Antimicrobial Drugs and Determination of Fecal Contamination Levels in Mangrove Oysters (Crassostrea rhizophorae)
Provedor de dados: 52
Autores: Rubião,Cynthia Annes; Franco,Robson Maia; Mesquita,Eliana de Fátima Marques de; Miguel,Marco Antonio Lemos; Cabral,Claudius Couto; Fonseca,Ana Beatriz Monteiro.
ABSTRACT The aim of this study was to determine and compare the Most Probable Number (MPN) of Total Coliforms (TC), Escherichia coli and Enterococcus spp. and to characterize the antimicrobial resistance profiles of Enterococcus spp. isolated from oysters collected in the Barra de Guaratiba Mangrove, Rio de Janeiro, Brazil. The enumeration of E. coli has been used to indicate fecal contamination and hygienic-sanitary conditions of bivalve molluscs. Enterococci are capable to transfer several antimicrobial resistance genes to pathogenic bacteria, including those from Gram-negative group. The oysters were bought from local fishermen and a total of 123 individuals were analyzed. The TC, E. coli and Enterococcus spp. MPN mean were 26,300/100 g, 3,260/100 g and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water pollution; Escherichia coli; Enterococcus spp.; Antimicrobial resistance; Bivalve molluscs.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100308

Consequences of the magnetic field, sonic and radiofrequency waves and intense pulsed light on the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Meyer,Patricia Froes; Santos-Filho,Sebastião David; Ronzio,Oscar Ariel; Bonelli,Ludmila; Fonseca,Adenilson de Souza da; Costa,Iris do Ceu Clara; Brandão Neto,José; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario.
Sources of magnetic field, radiofrequency and audible sonic waves and pulsed light have been used in physiotherapy to treat different disorders. In nuclear medicine, blood constituents(Bl-Co) are labeled with technetium-99m (99mTc) are used. This study evaluated the consequences of magnetic field, radiofrequency and audible sonic waves and intense pulsed light sources on the labeling of Bl-Co with 99mTc. Blood from Wistar rats was exposed to the cited sources. The labeling of Bl-Co with 99mTc was performed. Blood not exposed to the physical agents was used(controls). Data showed that the exposure to the different studied sources did not alter significantly (p&gt;0.05) the labeling of Bl-Co. Although the results were obtained with animals, the data suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood constituents; Magnetic field; Sonic and radiofrequency waves; Technetium-99m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600014

Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis
Provedor de dados: 52
Autores: Pagnoncelli,Maria Giovana Binder; Araújo,Nathália Kelly de; Silva,Nayane Macêdo Portela da; Assis,Cristiane Fernandes de; Rodrigues,Sueli; Macedo,Gorete Ribeiro de.
The chitosanase production by Paenibacillus ehimensis was studied in submerged cultures and the chitosan hydrolysis was evaluated by using these enzymes without purification. The bacterium produced inducibles enzymes after 12 h of growth in a culture medium containing 0.2% (w/v) of soluble chitosan as carbon source. The enzyme production was strongly repressed by the presence of glucose. The production started as soon as the available sugars finished in the culture medium. The maximum level of chitosanase activity was 500 U.L-1 at 36°C after 36 h incubation. The crude enzyme was optimally active at pH 6.0 and 55°C and in these conditions, the enzyme presented good stability (6 days). The enzyme without purification was used to hydrolyze the chitosan which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitosanase; Paenibacillus ehimensis; Chitosanase production; Crude enzymes and TLC.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600023

Intensity of bitterness of processed yerba mate leaves originated in two contrasted light environments
Provedor de dados: 52
Autores: Rakocevic,Miroslava; Medrado,Moacir José Sales; Lucambio,Fernando; Valduga,Alice Teresa.
The bitterness intensity of beverage prepared from the leaves produced on the males and females of yerba mate (Ilex paraguariensis), grown in the forest understory and monoculture, was evaluated. The leaves were grouped by their position (in the crown and on the branch tips) and by the leaf age. The leaf gas exchange, leaf temperature and photosynthetic photon flux density were observed. Inter and intra-specific competition for light and self-shading showed the same effect on yerba mate beverage taste. All the shading types resulted in bitterer taste of the processed yerba mate leaves compared to the leaves originated under the direct sun exposure. The leaves from the plants grown in the monoculture showed less bitterness than those grown in the forest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf age; Photosynthesis; Shade; Stomatal conductance; Temperature; Transpiration.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300018

Cytogenetic studies in some species of Passiflora L. (Passifloraceae): a review emphasizing Brazilian species
Provedor de dados: 52
Autores: Souza,Margarete Magalhães; Pereira,Telma N. Santana; Vieira,Maria Lúcia Carneiro.
The Passifloraceae is represented by species of tropical and subtropical origin. The Passiflora is the richest genus with approximately 450 species, 200 of them being native to Brazil. Recent karyological studies have reported the basic chromosome number for the Passiflora genus as x = 6, whereas x = 9, x = 10 and x = 12 were established as secondary basic numbers. High rates of fertility occur in most Passiflora species, since both meiotic index and pollen viability are above 90%. Unusual meiotic behavior has been described in some taxa. Unviable pollen were observed in some diploids species. The genome size varies from 1.83 to 5.36 pg, and significant interspecific variance has been observed. Studies using the FISH methodology have shown that there are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Banding; Meiotic behavior; Pollen viability; Nuclear DNA content; FISH.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200003

Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor
Provedor de dados: 52
Autores: Patel,Seema; Kothari,Damini; Shukla,Rishikesh; Das,Debasish; Goyal,Arun.
The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman Design and a 2² full factorial Central Composite Design was employed. After response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. The experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediococcus pentosaceus; Dextran; Plackett-Burman Design; Central Composite Design; Response Surface Methodology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600007

Use of metabolism and swimming activity to evaluate the sublethal toxicity of surfactant (LAS-C12) on Mugil platanus
Provedor de dados: 52
Autores: Barbieri,Edison.
This study aimed to investigate the toxicological effects of the LAS-C12 on Mugil platanus (mullet). Fishes exposed to 1.0 mg.L-1 for 24, 48, 72, 96 and 120 hours presented significant increase in specific routine metabolism. At the concentration of 0.5 mgL-1, the active metabolism presented a decreasing trend from 48 h of exposure on. However, only the consumption averages for 72 h were statistically different from the ones obtained for other periods of exposure. The lowest oxygen consumption in this concentration was observed for 24, 48 and 72 hours of exposure. Significant differences between the control and the concentration of 2.5 mgL-1 were observed for the different periods of exposure. It was not possible to measure the consumption of oxygen for 96...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LAS; Swimming performance; Oxygen consumption; Metabolism; Detergents.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100012

Comparison between four and seven-day Ceriodaphnia dubia survival and reproduction test protocols using oil refinery effluent samples
Provedor de dados: 52
Autores: Oliveira-Filho,Eduardo Cyrino; Da-Matta,Anndressa Camillo; Cabral,Lucianna Lopes; Veiga,Letícia Falcão; Paumgartten,Francisco José Roma.
The aim of this study was to compare the 7-day Ceriodaphnia dubia survival and reproduction test with a 4 day test to evaluate the toxicity of oil refinery effluents. Both shown identical NOECs with three of 6 tested samples. With one of the tested samples, the 4-day NOEC was one tested-concentration lower, and with the remaining two samples, it was one tested-concentration higher than those obtained using the 7-day protocol. Then results suggested that 4 and 7-day protocols were comparable to detect toxic effects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic toxicology; Water pollution; Ecotoxicology; Oil refinery effluents; Reproduction; Aquatic chronic tests.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100017

Effect of relative humidities on microstructural, barrier and mechanical properties of Yam starch-monoglyceride films
Provedor de dados: 52
Autores: Ferreira,Fábio Avelino Bublitz; Grossmann,Maria Victória Eiras; Mali,Suzana; Yamashita,Fábio; Cardoso,Lisandro Pavie.
The effect of monoglyceride on microstructural, barrier and mechanical properties of casted yam starch films were investigated in different relative humidities (RH) and compared with glycerol-starch films. A single screw extruder was used to produce the starch - monoglyceride complex before film production and this process was effective to inhibit the phase separation in films. The addition of the hydrophobic compound reduced hydrophobicity, transparency and water vapor permeability of films. This later value for starch-glycerol film (1.7 x 10-10 g Pa-1 s-1 m-1) was higher than starch (1.2 x 10-10 g Pa-1 s-1 m-1) and monoglyceride-starch films (1.0 x 10-10 g Pa-1 s-1 m-1). Films containing glycerol had higher relative crystallinity (B and V H) with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradable films; Hydrophilicityandglycerol-monoestearate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600022

Proposal for a Monitoring and Dispatch System for Distributed Micro-Generation of Renewable Energy in Virtual Energy Centers
Provedor de dados: 52
Autores: Galvão,Rodrigo Regis de Almeida; França,Thiago José Lippo de; Pinheiro,Breno Carneiro; Lucio,Luís Thiago.
ABSTRACT The creation and updating of ANEEL (National Electricity Regulatory Agency) regulatory resolution on distributed generation provided a new business environment for the electricity sector, as well as enabling consumers to generate energy. In this context, distributed microgeneration plants management is a challenge, mainly for electric power distributors and for the entire supply chain and services. In this sense, it is urgent to develop a monitoring and dispatch system in microgeneration plants, in order to optimize the capacity factor of the enterprises and to enable the creation of Virtual Power Plants (VPP). VPPs are part of a new dynamic of the energy sector's strategic environment, which strengthens distributed generation through smart meters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distributed Micro-Generation; Electric Power Market; Virtual Power Plants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200213

Phenotypic Diversity of Apple (Malus sp.) Germplasm in South Serbia
Provedor de dados: 52
Autores: Mratinić,Evica; Akšić,Milica Fotirić.
To determine the overall degree of polymorphism, detect similarities among the accessions and collect highly valued genotypes of native apples, 21 characters of 18 autochthonous apple cultivars were studied for three years. Particular individual accessions were selected from the south region of Serbia according to economically valuable characters and tested in situ. Agronomic evaluation of germplasm accessions revealed considerable diversity among different accessions for all the characters studied. The analysis of variance revealed significant differences among the accessions and among years for some characters as well. Cluster analysis distinguished Malus sp. accessions into three distinct groups. Despite of the significant differences among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple; Cluster analysis; Diversity; Genetic resources.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300004

Silymarin-Laden PVP-Nanocontainers Prepared Via the Electrospraying Technique for Improved Aqueous Solubility and Dissolution Rate
Provedor de dados: 52
Autores: Yousaf,Abid Mehmood; Malik,Usman Rashid; Shahzad,Yasser; Hussain,Talib; Khan,Ikram Ullah; Din,Fakhar Ud; Mahmood,Tariq; Ahsan,Hafiz Muhammad; Syed,Ahmed Shah; Akram,Muhammad Rouf.
Abstract The aim of the present research was to develop a silymarin-laden PVP-nanocontainer providing ameliorated aqueous solubility and dissolution of the drug. Several silymarin-laden formulations were formed with varying quantities of PVP and SDS via the solvent evaporation method using the electrospraying technique. The influence of the hydrophilic carriers on solubility and dissolution was explored. The solid-state characterization was carried out by particle-size analysis, PXRD, DSC, FTIR and SEM. All of the formulations demonstrated better solubility and dissolution than did silymarin plain powder. Both the SDS and PVP had positive effects on solubility and dissolution of silymarin in the aqueous media. An increased solubility was attained as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silymarin; Electrospraying; PVP-nanocontainers; Aqueous solubility; Dissolution.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100612

Glucose administration inhibits the hepatic activation of gluconeogenesis promoted by insulin-induced hypoglycemia
Provedor de dados: 52
Autores: Galende,Sharize Betoni; Oliveira Neto,Oswaldo Custódio de; Santos,Leandro Freire dos; Peicher,Márcia Vardanega; Souza,Helenir Medri de; Bazotte,Roberto Barbosa.
The activation of hepatic gluconeogenesis in male Wistar adult 6 h fasted rats during insulin-induced hypoglycemia (IIH) was previously demonstrated. In this study, the effects of intraperitoneal (ip) glucose (100 mg/kg) on the activation of liver gluconeogenesis during IIH was investigated. Thus, 6 h fasted rats that received ip regular insulin (1 U/kg) and 30 min later ip saline (Control group) or glucose (Experimental group) were compared. All the experiments were executed 60 min after insulin injection. The glycemia of Control and Experimental groups were not different (P &gt; 0.05). To investigate gluconeogenesis, liver perfusion experiments were performed. The results demonstrated that excepting glycerol, livers from rats which received ip glucose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoglycemia; Glycemia recovery; Hepatic metabolism; Gluconeogenesis; Ureagenesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400008

Dietary vitamin E supplementation on cholesterol and cholesterol oxides of pig meat and cooked ham
Provedor de dados: 52
Autores: Souza,Vera Lúcia Ferreira de; Silva,Rui Sérgio S. Ferreira da.
Objective of this work was to evaluate the protective effect of vitamin E on the cooked ham. Ninety-six pigs (LW X L X P), forty-eight barrows and forty-eight gilts were divided in four randomized blocks. Each block received four treatments: a control diet, diets formulated with 100 mg, with 200 mg and with 400 mg of vitamin E/kg diet. The average cholesterol values in cooked ham were 46.53 ± 0.47 mg/100g. However, a reduction of 30% was observed in samples of supplemented diets with 400 mg of vitamin E/kg. During shelf-life of the cooked ham was observed a reduction in the cholesterol levels, with the associated production of cholesterol oxides. The cholesterol oxides observed were: cholesta-4,6-dien-3-one; 20alpha-hydroxycolesterol;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesta-4; 6-dien-3-one 20alpha-hydroxycolesterol 24-hydroxycholesterol.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300003

Isolation of Rhodocyclus gelatinosus from poultry slaughterhouse wastewater
Provedor de dados: 52
Autores: Ponsano,Elisa Helena Giglio; Lacava,Pedro Magalhães; Pinto,Marcos Franke.
Four cultures of photosynthetic bacteria isolated from poultry slaughterhouse wastewater were identified as Rhodocyclus gelatinosus based on the following properties: reddish color of cultures in synthetic medium, presence of motility, slightly curved Gram-negative rods morphology, gelatin liquefying activity, utilization of citrate as carbon source and production of bacteriochlorophyl a and carotenoids of the spirilloxanthin alternative series. R. gelatinosus may represent a source of nutrients and pigments with application in poultry feed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photosynthetic bacteria; Rhodocyclus gelatinosus; Poultry slaughterhouse wastewater.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600007

Application of Chinese Jun-Cao technique for the production of Brazilian Ganoderma lucidum strains
Provedor de dados: 52
Autores: Rolim,Leonardo do Nascimento; Sales-Campos,Ceci; Cavalcanti,Maria Auxiliadora de Queiroz; Urben,Arailde Fontes.
Ganoderma lucidum is a medicinal mushroom traditionally used in China against a wide range of diseases such as cancer and also for its prevention. In this work, commercial Chinese strains G. lucidum were compared to wild Brazilian strains aiming to determine the cultivation potential through the use of Jun-Cao. Six formulations were tested and the strains presented good response to the applied method. In general, the mixture between the grass and wood was well suited for the basidiomycetes, contributing to the preparation of substrates that generated better results in Jun Cao.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mushrooms; Ganoderma lucidum; Jun-Cao technique.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300009

Erratum: The Evolving Role of Erythropoietin in Lung of Irradiated Rats
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100916

Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
Provedor de dados: 52
Autores: Moreira,Mônica; Adamoski,Douglas; Sun,Jiufeng; Najafzadeh,Mohammad Javad; Nascimento,Mariana Machado Fidelis do; Gomes,Renata Rodrigues; Barbieri,Dicler de Sant'Anna; Glienke,Chirlei; Klisiowicz,Débora do Rocio; Vicente,Vânia Aparecida.
The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Padlock probe; RCA; Streptococcus mutans.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054

Biotechnological potential of alternative carbon sources for production of pectinases by Rhizopus microsporus var. rhizopodiformis
Provedor de dados: 52
Autores: Damásio,André Ricardo de Lima; Maller,Alexandre; Silva,Tony Márcio da; Jorge,João Atílio; Terenzi,Hector Francisco; Polizeli,Maria de Lourdes Teixeira de Moraes.
Fungi collected from Brazilian soil and decomposing plants were screened for pectinase production. R. microsporus var. rhizopodiformis was the best producer and was selected to evaluate the pectic enzyme production under several nutritional and environmental conditions. The pectinase production was studied at 40ºC, under 28 carbon sources-supplemented medium. The inducer effect of several agro-industrial residues such as sugar cane bagasse, wheat flour and corncob on polygalacturonase (PG) activity was 4-, 3- and 2-fold higher than the control (pectin). In glucose-medium, a constitutive pectin lyase (PL) activity was detected. The results demonstrated that R. microsporus produced high levels of PG (57.7 U/mg) and PL (88.6 U/mg) in lemon peel-medium. PG had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agro industrial residues; Lemon peel; Pectin lyase; Polygalacturonase; R. microsporus var. rhizopodiformis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100019

Analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum
Provedor de dados: 52
Autores: Del-Vechio-Vieira,Glauciemar; Sousa,Orlando Vieira de; Miranda,Mariza Abreu; Senna-Valle,Luci; Kaplan,Maria Auxiliadora Coelho.
The chemical composition, analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum were investigated. The main compounds found in the essential oil were germacrene D, α-humulene and β-cedrene. The oil, with LD50 of 2.50 g/kg, inhibited the acetic acid-induced writhing at the dose of 200 mg/kg. In the formalin test, the oil inhibited the first phase (200 mg/kg) and the second phase (100 mg/kg and 200 mg/kg). In the hot plate test, after 30 and 60 min of treatment the doses of 100 and 200 mg/kg increased the reaction time. The antiedematogenic effect, reduction on the exudate volume and leukocyte mobilization were observed at the doses of 100 and 200 mg/kg. The results indicated that A. fastigiatum possessed the analgesic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ageratum fastigiatum; Essential oil; Nociception; Inflammation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500008

Influence of the presence and type of fragrance on the sensory perception of cosmetic formulations
Provedor de dados: 52
Autores: Gonçalves,Gisele Mara Silva; Srebernich,Silvana Mariana; Vercelino,Bartira Guiçardi; Zampieri,Bruna Melli.
The aim of the present study was to evaluate the sensory assessments of identical cosmetic formulations with and without fragrance to investigate not only the acceptance but also how different fragrances affected their attributes, such as skin feel, tackiness and spreadability. Three gel and three cream formulations with and without two types of fragrance, fennel and sweet flowers, were assessed for various attributes. The presence and type of fragrance used affected the testers' perception of some attributes, showing that the influence of this component should not be disregarded. Apparently, a consumer's reaction to a cosmetic product is not only based on its efficacy but also on how its attributes are perceived, such as appearance, skin feel and smell.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetics; Development; Fragrance; Olfactory memory; Sensory.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200005

Effect of Preheating on Microstructure and Tensile Properties of Friction Stir Welded AA7075 Aluminium Alloy Joints
Provedor de dados: 52
Autores: Poongkundran,R.; Senthilkumar,K.
ABSTRACT The high strength AA7075 aluminum alloy is commonly used in the aerospace components due to its exclusive mechanical properties like lightweight and high strength. This alloy cannot be welded by fusion welding techniques due to solidification cracking which severely degrade the mechanical properties of the joint. In contrast, through friction stir welding (FSW) process solidification relate defects can be eliminated. Anyhow, the strength of friction stir welded joint is influenced by process parameters and tool parameters. These parameters govern the heat input, metal flow, microstructure evolution and mechanical properties of the weld. In normal welding condition, (without preheating) heat is generated by friction force which is produced between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum alloy; Preheating; Friction stir welding; Tensile strength; Microstructure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300404

Effects of lymphatic drainage on cellulitis assessed by magnetic resonance
Provedor de dados: 52
Autores: Meyer,Patrícia Froes; Martins,Nara Mendes; Martins,Flávia Mendes; Monteiro,Rosimary Araújo; Mendonça,Karla Morganna Pereira Pinto de.
The aim of this study was to assess the use of magnetic resonance as a new method for evaluating the manual lymphatic drainage technique in treating cellulitis. Cellulitis is one of the main esthetic problems that lead women toward seeking guidance and specific treatments. There are various therapeutic approaches, owing to the multi-factorial nature of its pathogen, although the effectiveness of most of these has not been definitively proven, given that the assessment methods used are mostly subjective or do not provide enough information on subcutaneous tissue. The introduction of magnetic resonance as a means of assessing a lymphatic drainage technique in cellulitis treatment makes the evaluation more accurate, since it enables a detailed study of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulitis; Lymphatic drainage; Magnetic resonance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700036

Growth of microalgae Scenedesmus sp in ethanol vinasse
Provedor de dados: 52
Autores: Ramirez,Nelzy Neyza Vargas; Farenzena,Marcelo; Trierweiler,Jorge Otávio.
This study evaluated the feasibility of using vinasse as a nutrient source for microalgae cultivation. The Scenedesmus sp was grown in a medium supplemented with vinasse and process variables were optimized using a factorial design and a Central Composite Design (CCD). The factorial design results showed that it was possible to cultivate microalgae at concentrations of up to 40% of vinasse in the culture medium. The CCD results showed that the light intensity and vinasse concentration influenced the amount of biomass produced.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vinasse; Microalgae; Scenedesmussp; Photobioreactor air-lift.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500630

Age and growth of Pimelodella pappenheimi (siluriformes, pimelodidae) from an Atlantic Forest Stream in Southern Brazil
Provedor de dados: 52
Autores: Amaral,Marina Faria do; Aranha,José Marcelo Rocha; Menezes,Márcia Santos de.
Studies were performed to determine the growth curves in length and weight for Pimelodella pappenheimi in the "das Pombas" stream, a coastal stream susceptible to the flash flood phenomenon. Samples were collected from September-95 to February-97, monthly, using electric fishing. The fishes were measured in total length and weight. A total of 156 pairs of otholits were taken for ring reading. The Ford-Walford transformation allowed the use of von Bertallanffy expression. The pale ring formation was annual, and it occurred between the summer and the autumn. This species grews in length according to Lt=15.86 (1-e-0.57(t+0.1551) ) equation and in weight according to Wt= 24.23 (1-e-0.57(t+0.1551) )3.12. The increase in length was faster until the second year...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Coastal Stream; Pimelodidae.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400010

Trioecy in Coccoloba cereifera Schwacke (Polygonaceae), a narrow endemic and threatened tropical species
Provedor de dados: 52
Autores: Silva,Celice Alexandre; Oliva,Marco Antonio; Vieira,Milene Faria; Fernandes,Geraldo Wilson.
Trioecy, the co-occurrence of the males, females, and hermaphrodite morphs in natural populations, is a rare and poorly studied breeding system. It is expressed in Coccoloba cereifera, an endemic, and endangered species from the rupestrian fields of Serra do Cipó, southeastern Brazil. Male individuals produce staminate flowers but no fruits. Female individuals produce pistillate flowers and set fruits. Both staminate and pistillate flowers present non-functional organs of the opposite sex that simulate perfect flowers. Hermaphrodite individuals produce two different perfect flowers, each one belonging to distinct individuals, and set fruits. Perfect flowers differ in the amount of pollen produced, in pollen viability, and in some morphological traits. Two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agamospermy; Breeding systems; Coccoloba cereifera; Sex determination; Sexual morphs; Trioecy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500017

Modelling the steady state and dynamic conditions of a biotrickling filter treating styrene and acetone in air
Provedor de dados: 52
Autores: Gerrard,Andrew Mark; Havranek,Josef; Novak,Vratislav; Halecky,Martin; Jones,Kim; Soccol,Carlos Ricardo; Paca,Jan.
The aim of this work was the study a trickling biofilter, where water was circulated throughout the bed. In the first steady state experiment, the packing materials used were 25mm Pall rings. The airflow rate was increased gradually and the concentration of styrene in the air stream was held constant. In the second experiment, 15mm Pall rings were used. In this case, the feed contained both styrene and a small amount of acetone. The concentration of acetone and the air flow rate were kept constant, but the styrene inlet concentration was increased. The concentrations were measured at the input, and also at an intermediate and the outlet position in the biotrickling filter to determine the concentration profile along the reactor. Using the values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotrickling filter; Steady state; Unsteady state; Mathematical models; Styrene; Acetone.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500028

Spirulina platensis Enhances the Beneficial Effect of Exercise on Oxidative Stress and the Lipid Profile in Rats
Provedor de dados: 52
Autores: Mazzola,Daiane; Fornari,Fernando; Vigano,Gabriela; Oro,Tatiana; Costa,Jorge Alberto Vieira; Bertolin,Telma Elita.
ABSTRACT This study aimed to evaluate the effect of Spirulina platensis and moderate exercise on oxidative stress and lipid profiles in the rats. Forty male Wistar rats were allocated to the following 10-week treatments, three times a week: exercise (E, 30 min swimming),S. platensis (SP, 26 mg/Kg); exercise andSpirulina (ES); and control (C). Outcomes were Thiobarbituric Acid Reactive Substances (TBARS) in serum and brain, and cholesterol and triglycerides (TG) in serum. Rats treated with exercise showed lower brain TBARS than the controls, mostly in association withS. platensis. In the groups E and ES, serum TBARS decreased after intervention. Compared with the controls, both E and ES prevented an increase in cholesterol and reduced triglycerides. Results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Antioxidant; Cholesterol; Lipid peroxidation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600961

Forecasting the Electricity Consumption in a Higher Education Institution
Provedor de dados: 52
Autores: Pepplow,Luiz Amilton; Betini,Roberto C.; Pereira,Thulio C. G.
Abstract The objective of this paper is to present a mathematical representation by Regression Analysis that enables the projection of electricity consumption according to the built area and population in Higher Education Institutions (HEI) and to define an Indicator that contemplates the most significant variable in consumption. of electric power. The Null Hypothesis -H0 is that in a HEI the most appropriate indicator is the Kilo Watt Hour per square meter (kWh / m2) as proposed by the Ministry of Planning and Management - MP. The research universe is 2,368 HEI, identified in a report from the Ministry of Education (2015). As Sample and case study, data from the thirteen Campi of the Federal Technological University of Paraná (UTFPR) are used. As a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indicators of electricity consumption; Regression analysis; Decision making.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200203

Enhanced production of alkaline protease by a mutant of Bacillus licheniformis N-2 for dehairing
Provedor de dados: 52
Autores: Nadeem,Muhammad; Qazi,Javed Iqbal; Baig,Shahjahan.
The purpose of the present investigations was to improve the yield of alkaline protease for leather dehairing by subjecting the indigenous proteolytic strain Bacillus licheniformis N-2 to various mutagenic treatments viz. UV irradiations, NTG (N-methyl-N-nitro-N-nitrosoguinidine) and MMS (methyl methane sulfonate). After screening on skim milk agar plates, a total of nine positive mutants were selected for shake flask experiments. Among these, the best proteolytic mutant designated as UV-9 showed 1.4 fold higher alkaline protease activity in preoptimized growth medium than the parent strain. The fermentation profile and kinetic parameters such u(h-1), Yp/s, Yp/x, Yx/s, q s, Qs, q p and Qp also indicated the superiority of the selected mutant UV-9 for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus mutants; Mutagenic treatments; Alkaline protease; Dehairing agent.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500003

Extraction model of low methoxyl pectin from apple pomace effects of acid concentration and time on the process and the product
Provedor de dados: 52
Autores: Fertonani,Heloísa Cristina Ramos; Scabio,Ardalla; Carneiro,Eliana Beleski Borba; Schemim,Maria Helene Canteri; Nogueira,Alessandro; Wosiacki,Gilvan.
In Brazil, one of the top apple producing countries in the world, apple processing is an increasing activity, with pomace as the main by-product. To extract pectin from pomace, factors affecting process and product should be studied for optimization. A model to produce LMP directly from dried apple pomace was established observing the effects of HNO3 concentration and the time of reaction at 97ºC, analyzed from a statistical and practical point of view. The model for gravimetric yield (R² =0.9834) predicts the highest value of 20.07 g/100 g (126 mM; 14.07 min) of a pectin with a degree of esterification of 48.49%. The model for degree of esterification of extracted pectin (R²= 0.9797) predicts the lowest value of 43.73% (200 mM; 10.07 min) with a yield of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple; Pectin; Extraction; LMP; Nitric acid.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100023

Functional cytology of the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) under osmotic stress
Provedor de dados: 52
Autores: Díaz,Ana Cristina; Sousa,Liliana Graciela; Petriella,Ana María.
The present work describes the effect of different salinities on the functional morphology of the P. argentinus hepatopancreas and analyses the tissue recovery after re-acclimation to freshwater. Adult prawns of both sexes at sexual rest were collected from a tributary of the Mar Chiquita coastal lagoon. The prawns were acclimated in aquaria to four salinity conditions: 0 (control), 8, 16 and 24‰. To evaluate the possible tissular recovery, after 60 days individuals from all the treatments were gradually acclimated to freshwater and maintained for other 30 days. Hepatopancreas samples were processed at the beginning of the trial and every 30 days using standard histological techniques for OM and TEM. The individuals from all the treatments, except the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salinity; Histology; Hepatopancreas; P. argentinus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300013

Immune response after rabies oral immunization in mice
Provedor de dados: 52
Autores: Silva,Camila Sloboda Pacheco da; Krüger,Ernesto Renato; Agottani,Jorge Victor Bacila; Oliveira,Rubens Chaguri de; Pereira,Sandro; Penha,Tânia Regina; Ollhoff,Rüdiger Daniel.
The objective of this study was to formulate an anti-rabies oral vaccine from the cell culture vaccine PV TECPAR to elicit the production of antibodies against the rabies in mice. A vaccine was developed using 10(7.5) DL50/0.03 ml viral antigens homogenised in lanovaseline to facilitate oral administration. Mice were vaccinated two times for seroconversion. Sera of the vaccinated mice showed a higher level of antibody production than the control group. These results could be used to direct the development of an anti-rabies oral vaccine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Oral vaccine; Mice; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600006

Hydrology and phytoplankton community structure at Itamaracá-Pernambuco (Northeast Brazil)
Provedor de dados: 52
Autores: Koening,Maria Luise; Macêdo,Silvio José de.
Quali-quantitative studies and hydrologic parameters were carried out in the profiles 6 (Orange) and 7 (Catuama) during the Victor Hensen cruise, in accordance with the bilateral scientific cooperation agreement Brazil/Germany. Hydrologically a zone of thermic and saline stability characterizes the superficial layer. The nutrient concentrations were generally low on the surface and higher at levels surpassing 100m in depth. 102 taxa were identified including diatoms (49), dinoflagellates (49), bluegreen algae (3), and euglenophyceae (1). The diversity and evenness were high, surpassing the environmental equilibrium. The clustering of samples showed evidence of 2 main groups, one encompassing the stations 32 and 38, characterized predominantly by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrology; Nutrients; Phytoplankton structure.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400002

Influence of bleaching technologies on the aerobic biodegradability of effluents from Eucalyptus kraft pulps factories
Provedor de dados: 52
Autores: Vidal,Gladys; Soto,Manuel; Méndez,Ramón; Lema,Juan Manuel.
Aerobic biodegradability of effluents from different Eucalyptus kraft pulp bleaching processes was studied. Bleaching effluents were obtained from: i) Chlorine Bleaching (CB) processes, with partial substitution of chlorine by chlorine dioxide and ii) Total Chlorine Free (TCF) processes. The overall biodegradability, in terms of Chemical Oxygen Demand (COD) was higher for TCF effluents (96-98%) than for CB ones (82-93%). Taking into account the higher organic load of CB effluents, this fact implied a much higher residual COD for them (100-180 mg/L) than for TCF effluents (10-30 mg/L). Furthermore, a refractory fraction of molecular weight higher than 43,000 Da was found in CB effluent, which implied the necessity of a further specific treatment. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kraft pulp bleaching wastewater; Aerobic biodegradation; Pulp bleaching technology; TCF; CB.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300009

First Report of Citrus Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae) in the State of Paraná, Brazil
Provedor de dados: 52
Autores: Molina,Rúbia de Oliveira; Nunes,William Mário de Carvalho; Gil,Luciano Grillo; Rinaldi,Dirlene Aparecida Moreno da Fonseca; Croce Filho,José; Carvalho,Regina Célia Zonta de.
The citrus blackfly Aleroucanthus woglumi Ashby, (Hemiptera Aleyrodidae) is an important pest that occurs in citrus groves, native to south-east Asia. In Brazil, according to the Ministry of Agriculture, this is a quarantine pest (A2) under official control IN 52, 2007 (MAPA) and is not widespread in the country. The insect can infest more than 300 host plants, including cultivated plants, ornamentals and weeds, but mostly occurs in the plants of the genus citrus. This paper is the first report of citrus blackfly in the State of Paraná.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Citrus pest; Aleyrodid.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400472

The lysosomal stability as a biomarker for the determination of pollution in aquatic environments
Provedor de dados: 52
Autores: Nazar,Maria Loreto; Rodrigues,Luiz Erlon Araujo; Nascimento,Iracema.
This work studied the effects caused by five different formulae of gasoline on the stability of the lysosomes isolated from the liver of the tilapia fish (Oreochromis niloticus). The functional integrity of the lysosomal membranes was evaluated via the acid phosphatase activity. The results showed that there were significant changes in the stability of the lysosomes exposed to the presence of the hydrocarbons in the environment. Therefore, considering the method's simplicity, the sensitivity of the responses and its low cost the assessment of the lysosomal activity could be an important tool for the study of the effects of pollution in the aquatic environments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Acid phosphatase; Lysosomes; Aquatic environments; Pollution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500025

Production of Polyhydroxybutyrate by Bacillus axaraqunsis BIPC01 using Petrochemical Wastewater as Carbon Source
Provedor de dados: 52
Autores: Mayeli,Nasim; Motamedi,Hossein; Heidarizadeh,Fariba.
The aim of this study was to use petrochemical wastewater as the source of carbon for the production of polyhydroxyalkanoates (PHA) in an effort to decrease its cost of production. For this purpose, PHA producing bacteria were isolated from the petrochemical wastewater of Bandar Imam, Iran. The purified colonies were screened for PHA by Sudan Black B and Nile Blue A staining. Among positively stained bacteria, the best PHA producer was selected on the basis of cell growth, PHA content and the monomer composition of PHA. The phenotypic and genotypic identification this isolate showed it to be Bacillus axaraqunsis. The PHA was produced at a cell density of about 9.46 g/l of maximum concentration of 6.33g/l l, corresponding to 66% of cell dry weight. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxyalkanoate; Petrochemical wastewater; Bacillus axaraqunsis; Biodegradable polymer; Bioplastic.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400643

Comparative leaf morphology and anatomy of three Asteraceae species
Provedor de dados: 52
Autores: Milan,Patricia; Hayashi,Adriana Hissae; Appezzato-da-Glória,Beatriz.
The objective of this paper was to describe and compare the morphology and anatomy of mature leaves of Mikania glomerata Spreng., Porophyllum ruderale Cass. and Vernonia condensata Baker (Asteraceae) species that have different habits emphasizing their secretory structures. Longitudinal and transversal sections of mature leaf blades of the three species were analyzed at the apex, base, and medium third part of the midvein of the leaf blade and of the margin. M. glomerata had uniseriate glandular trichomes and secretory ducts; P. ruderale had hydathodes and secretory cavities; and V. condensata had idioblasts and uni-and biseriate glandular trichomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mikania glomerata; Porophyllum ruderale; Vernonia condensata; Secretory structures; Medicinal plants.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100016

Converting a Conventional Vehicle into an Electric Vehicle (EV)
Provedor de dados: 52
Autores: Silva,Jardel Eugenio da; Urbanetz Junior,Jair.
Abstract The electric vehicle (EV) is not a recent invention. Between the end of the XIX century and the beginning of the XX century, most motor vehicles were electric, due to their superior reliability and cleanliness, compared to that of vehicles driven by internal combustion engines (ICE). However, with the development of ICEs and the reduction of their price, electric cars were forgotten. Only almost a century later, they returned in the market due to a significant increase in fossil fuels prices, as well as to a growing environmental concern. EVs present a number of advantages over ICE vehicles: they are simpler and require less use and replacement of parts, resulting in lower maintenance costs; moreover, they do not release pollutants into the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electric vehicles; Photovoltaic panels; Environment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200206

Environmental variables and tree population structures in deciduous forests of central Brazil with different levels of logging
Provedor de dados: 52
Autores: Vieira,Daniel Luis Mascia; Scariot,Aldicir.
Population structures of six tree species in three fragments of intact seasonal deciduous forest and three fragments disturbed by logging were studied in the northeastern Goiás. Forty random 400 m² plots were allocated in each fragment to survey plant population structures, number of stumps, cattle feces, burnt logs, and canopy openness. Soil cover by life forms was estimated in 1m² sub-plots. Lianas were abundant in intermediately logged fragments and invasive herbs in the most disturbed fragment. Cattle avoided dense herbaceous strata, such as liana tangles. Cavanillesia arborea, Eugenia dysenterica and Swartzia multijuga trees occurred at very low densities in all the fragments and their seedlings were practically absent, which might endanger their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seasonal forest; Canopy openness; Plant cover; Cattle grazing; Lianas.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200024

Evaluation of DSSAT soil-water balance module under cropped and bare soil conditions
Provedor de dados: 52
Autores: Faria,Rogério Teixeira de; Bowen,Walter Truman.
The performance of the soil water balance module (SWBM) in the models of DSSAT v3.5 was evaluated against soil moisture data measured in bare soil and dry bean plots, in Paraná, southern Brazil. Under bare soil, the SWBM showed a low performance to simulate soil moisture profiles due to inadequacies of the method used to calculate unsaturated soil water flux. Improved estimates were achieved by modifying the SWBM with the use of Darcy's equation to simulate soil water flux as a function of soil water potential gradient between consecutive soil layers. When used to simulate water balance for the bean crop, the modified SWBM improved soil moisture estimation but underpredicted crop yield. Root water uptake data indicated that assumptions on the original...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soil moisture; Dry Bean; Modeling.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400001

Protective Effects of Sodium Selenite and Vitamin E on Mercuric Chloride-Induced Cardiotoxicity in Male Rats
Provedor de dados: 52
Autores: Karaboduk,Hatice; Uzunhisarcikli,Meltem; Kalender,Yusuf.
This study was designed to investigate the protective effects of sodium selenite and/or vitamin E against mercuric chloride-induced cardiotoxicity. Male Wistar rats (n=48, 310±10 g) were administered mercuric chloride (1.0 mg/kg bw), sodium selenite (0.25 mg/kg bw), vitamin E (100 mg/kg bw), sodium selenite plus mercuric chloride, vitamin E plus mercuric chloride and sodium selenite plus vitamin E plus mercuric chloride daily via gavage for four weeks. Malondialdehyde (MDA) level, antioxidant enzyme activities [total superoxide dismutase (SOD), catalase (CAT), total glutathione peroxidase (GPx) and total glutathione-S-transferase (GST)], and histopathological changes in the heart tissue were evaluated. Results showed that mercuric chloride exposure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercuric chloride; Sodium selenite; Vitamin E; Cardiotoxicity; Oxidative Stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200229

Chemotaxonomic Fingerprinting of Chilean Lichens Through Maldi and Electrospray Ionization Mass Spectrometry
Provedor de dados: 52
Autores: Santos,Leonardo Silva; Soriano,Maria del Pilar Camarantin; Mirabal-Gallardo,Yaneris; Carrasco-Sanchez,Veronica; Nachtigall,Fabiane Manke; Pereira,Iris; Pereira,Eugenia.
The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chilean lichens; Chemotaxonomic; Fingerprint; MALDI-TOF-MS; UPLC-ESI(-)-MS.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200244

Impacts of the construction of the Port of Suape on phytoplankton in the Ipojuca River estuary (Pernambuco-Brazil)
Provedor de dados: 52
Autores: Koening,Maria Luise; Leça,Enide Eskinazi; Neumann-Leitão,Sigrid; Macêdo,Silvio José de.
In order to address the impact on phytoplankton, sampling was conducted monthly at 4 fixed stations, from April/86 to March/87 at diurnal low and high tide using a plankton net (65 mum mesh size) and a 1 L Van Dorn bottle. Among the 133 taxa identified, marine littoral euryhaline species were most common, outranking Gyrosigma balticum (Ehrenberg) Rabenhorst, Nitzschia sigma (Kützing) Wm. Smith, Licmophora abbreviata Agardh, Climacosphenia moniligera Ehrenberg, Surirella febigerii Lewis, Terpsinoe musica Ehrenberg and Cylindrotheca closterium (Ehrenberg) Reiman and Lewis. The port construction caused significant changes to the phytoplankton community with a strong influence of marine species (mainly dinoflagellate) because of the opening of the reef near...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Phytoplankton; Environmental impacts; Estuary; Suape Port.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100012

Effect of biologically treated petroleum sludge on seed germination and seedling growth of Vigna unguiculata (L.) Walp. (Fabaceae)
Provedor de dados: 52
Autores: Sangeetha,Jeyabalan; Thangadurai,Devarajan.
The present investigation was carried out to study the response of different concentrations of treated petroleum sludge on seed germination, root and shoot length and tolerance of Vigna unguiculata (L.) Walp. The biologically treated petroleum sludge with bacterial consortium showed 54.8% reduction in total petroleum hydrocarbons. Treated sludge was utilized with agricultural soil in known concentration for the assessment of growth of V. unguiculata. A remarkable absence of seed germination was observed at higher sludge concentration. The different concentrations of treated petroleum sludge showed severe decline on the length, weight and vigour index of the tested seedlings with increasing sludge concentrations. The results showed that the difference in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial consortium; Biodegradation; Petroleum sludge; Seed germination; Total petroleum hydrocarbon; Vigna unguiculata.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300017

Lentinan promotes the root of Brassica CampestrisL.
Provedor de dados: 52
Autores: Zhang,Guoyun; Su,Liqiu; Cao,Aijia; Han,Guangjian; Bie,Zhenyu; Zhang,Jian; Li,Xinglin.
The aim of this work was to study the effect of lentinan on Brassica campestris L (rape). Spraying on the leaves of lentinan B. campestris L. at 0.05×10-6 g ml-1 concentration significantly promoted the root elongation (P＜0.05). The results for the first time showed that lentinan could prolongate roots as a new plant hormone.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lentinan; Oilseed rape; Promoting root.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300333

Structure and dynamics of phytoplankton community in the Botafogo reservoir-Pernambuco-Brazil
Provedor de dados: 52
Autores: Lira,Giulliari Alan da Silva Tavares de; Bittencourt-Oliveira,Maria do Carmo; Moura,Ariadne do Nascimento.
The aim of the present study was to investigate the structure and dynamics of the phytoplankton in the Botafogo reservoir-PE-Brazil. Phytoplankton assemblages were identified from current literature and density was estimated using an inverted microscope. Concurrently to the sampling of biotic variables, measurements of abiotic parameters, such as water temperature, dissolved oxygen and pH, were determined using field probes and transparency was determined with a Secchi disk. Total phosphorus and total nitrogen concentrations were determined in laboratory. A total of 24 taxa were identified. Chlorophyta presented the greatest number of species. Species diversity in the reservoir was low throughout the study period. Principal component analysis revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Structure; Tropical reservoir; Phytoplankton; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200029

Partial characterization of bacteriocins produced by Lactobacillus acidophilus and Pediococcus acidilactici
Provedor de dados: 52
Autores: Sivakumar,Nallusamy; Rajamani,; Saif,Al-Bahry.
Bacteriocin producing Lactobacillus acidophilus and Pediococcus acidilactici were isolated from milk and meat samples, respectively. An attempt was made to produce bacteriocin in a Dairy Based (DB) medium using these organisms. Higher bacteriocin activity was shown by L. acidophilus in the DB medium. Bacteriocins of both the organisms were effective against food pathogens. The bacteriocins were stable at pH 3 - 9 up to 24 h and active at 100ºC. The bacteriocins could be stored at -20ºC for at least 45 days, at 4ºC for 20 days and at 37ºC for 5 days.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriocin; Lactobacillus acidophilus; Pediococcus acidilactici.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500023

Experimental models using radionuclides for assessment of the effects of natural drugs
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Rebello,Bernardo; Rocha,Emely Kazan; Timóteo,Margareth de Oliveira; Olej,Beni; Bernardo Filho,Mário; Caldas,Luiz Querino de Araújo.
The aim of this study was to evaluate the effect of the extracts of Nectandra membranacea (N. membranacea), Ginkgo biloba (EGb) and Passiflora (PEF) on the morphology of red blood cells (RBC), on the biodistribution of sodium pertechnetate (99mTcO4Na), on the morphology of duodenum and on the labeling of blood constituents (BC, IF-P, IF-BC) with technetium-99m (Tc-99m). Morphometry studies also were performed. The results show that EGb promotes alteration of the labeling of BC, IF-P and IF-BC (p&lt;0.05). The N. membranacea extract does not promote significant alteration of the radiolabeling, and PEF extract alters the IF-P labeling. N. membranacea, EGb and PEF extracts were able to alter the RBC morphology (P&lt;0.05). N. membranacea extract and EGb...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nectandra membranacea (N. membranacea); Ginkgo biloba (EGb); Passiflora edulis flavicarpa (PEF); Blood constituents (BC) labeling; Technetium-99m; Biodistribution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700027

Effects of sublethal concentrations of tobacco (Nicotiana tobaccum) leaf dust on some biochemical parameters of Hybrid catfish (Clarias gariepinus and Heterobranchus bidorsalis)
Provedor de dados: 52
Autores: Adamu,Kabir Mohammed; Kori-Siakpere,Ovie.
The aim of this work was to study the effect of tobacco (Nicotiana tobaccum) leaf dust at sublethal concentrations (375.6, 187.8, 93.9 0.0mg/L) on biochemical parameters of Hybrid catfish in a static semi-renewable bioassay system with the aim to ascertain its effect on the test fish after 14 days exposure period. The effects of the plant dust on the test fish was dose-dependent, revealing significant difference in albumin, glucose, triglyceride, cholesterol, urea, and uric acid of the serum, liver and kidney; total protein and globulin of serum and kidney and creatinine and calcium of the serum while insignificant difference were observed for calcium and creatinine in liver and kidney and total protein and globulin in liver compared to the control. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tobacco leaf dust; Biochemical parameters; Hybrid catfish; Serum; Liver and kidney.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100023

Erratum: Investigation of the GJB6 Deletion Mutations Del (GJB6-D13s1830) and Del (GJB6-D13s1854) in Iranian Patients with Autosomal-Recessive Non-Syndromic Hearing Loss (ARNSHL)
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100907

Polyphenols From Cutch Tree ( Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity
Provedor de dados: 52
Autores: Kumar,Rakesh; Arora,Rohit; Mahajan,Jyoti; Mahey,Sonia; Arora,Saroj.
ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative stress in present work. Leaves of A. catechu were extracted with 80% methanol to get methanol extract (AME). It was assessed for antioxidant activity using DPPH, ABTS, CUPRAC, ferric ion reducing, superoxide scavenging and peroxyl radical scavenging assays. DNA protective activity was also investigated using plasmid nicking assay. Further, antiproliferative activity was determined using MTT assay in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acacia catechu; Antioxidant; Antiproliferative; UHPLC; MTT.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100303

Antioxidant properties of some filamentous green algae (Chaetomorpha Genus)
Provedor de dados: 52
Autores: Farasat,Massoumeh; Khavari-Nejad,Ramazan-Ali; Nabavi,Seyed Mohammad Bagher; Namjooyan,Foroogh.
The antioxidant activity and the contents of total phenolics and flavonoids were quantified in the methanolic extracts of four Chaetomorpha species including C. aerea, C. crassa, C. linum and C. brachygona. Eight samples of Chaetomorpha plants were collected from five locations along the northern coasts of the Persian Gulf in south of Iran from December 2010 until October 2011. Methanolic extracts of the seaweeds were assessed for their antioxidant activity using DPPH radical scavenging assay. C. linum showed highest antioxidant potential with a relatively low IC50 (1.484 ± 0.168 mg mL-1), the highest flavonoid content (18.177 ± 2.238 mg RE g-1 ) and a relatively high content of phenolics (2.895 ± 0.415 mg GAE g-1) in comparison with the other species. C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Phenolics; Flavonoid; DPPH; Green algae; Chaetomorpha.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600005

Anatomical aspects of IBA-treated microcuttings of Gomphrena macrocephala St.-Hil
Provedor de dados: 52
Autores: Moreira,Míriam F.; Appezzato-da-Glória,Beatriz; Zaidan,Lilian B.P.
Gomphrena macrocephala St.-Hil. (Amaranthaceae) is a perennial herb from the cerrado with medicinal properties and ornamental interest. Plants can be micropropagated through nodal segments, but acclimatization is difficult. The aim of this study was to establish a relationship between anatomical aspects of the roots and the acclimatization process. When cultures were supplemented with IBA, callus and thick and frangible roots appeared at the base of the microcuttings. A fragile vascular connection between roots and shoots was observed. Abnormal adventitious roots showing alteration in the vascular cylinder and hypertrophy of the cortical cells were also noted. These roots interfere in the transfer to extra vitrum conditions. When no growth regulator was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gomphrena; Root anatomy; In vitro rooting; Indol butiric acid; Vascular connection.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200012

Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues
Provedor de dados: 52
Autores: Linan,Lamia Zuniga; Lima,Nadson Murilo Nascimento; Benatti,Carol; Xavier,Mariana; Rodrigues,Ana A.; Manenti,Flavio; Jardini,Andre; Maciel Filho,Rubens; Gilioli,Rovilson.
ABSTRACT A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 µg MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PMMA; Synthesis; Cytotoxicity; Viability; Mesenchymal cells.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100601

External morphology of the adult of Heraclides anchisiades capys (Hübner, [1809]) (Lepidoptera - Papilionidae) III: abdomen
Provedor de dados: 52
Autores: Leite,Luis Anderson Ribeiro; Casagrande,Mirna Martins; Mielke,Olaf Hermann Hendrik.
The purpose of this study was to highlight the morphological components of the abdomen of the adults of Heraclides anchisiades capys (Hübner, [1809]), seeking a comparative focus with other Papilionoidea and Hesperioidea. The most relevant morphological characters were: absence of post-espiracular bar in both the sexes, presence of superuncus in the males as a projection of the eighth tergum and saccus with a posterior projection, bursa copulatrix with approximately 2.3 times the length of the ductus bursae in the females.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Neotropical; Papilioninae; Papilionoidea.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200015

Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract
Provedor de dados: 52
Autores: Naine,S. Jemimah; Devi,C. Subathra; Mohanasrinivasan,V.; B,Vaishnavi.
The main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces parvulus VITJS11 with a view to assess their therapeutic potential. The biological activity of ethyl acetate extract was tested against fungal and bacterial pathogens. The free radical scavenging potential of the crude extract was determined by DPPH assay. The chemo preventive properties of S. parvulus VITJS11 ethyl acetate extract was examined by MTT assay on HepG2 cells. The morphological, physiological and the biochemical properties of the strain S. parvulus VITJS11 was confirmed by conventional methods. Genotypic characterization was done using 16S r-DNA partial gene amplification and sequencing. The authenticity of the crude chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine actinomycetes; Streptomyces parvulusVITJS11; Antimicrobial activity; Antioxidants; Cytotoxicity; Bioactive compounds.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200198

Enhanced response of the fricke solution doped with hematoporphyrin under X-rays irradiation
Provedor de dados: 52
Autores: Austerlitz,Carlos; Souza,Vivianne Lúcia Bormann de; Campos,Diana Maria Tavares; Kurachi,Cristina; Bagnato,Vanderley; Sibata,Cláudio.
The vials filled with Fricke solutions were doped with increasing concentrations of Photogem®, used in photodynamic therapy. These vials were then irradiated with low-energy X-rays with doses ranging from 5 to 20 Gy. The conventional Fricke solution was also irradiated with the same doses. The concentration of ferric ions for the Fricke and doped-Fricke irradiated solutions were measured in a spectrophotometer at 220 to 340 nm. The results showed that there was an enhancement in the response of the doped-Fricke solution, which was proportional to the concentration of the photosensitizer. The use of such procedure for studying the radiosensitizing property of photosensitizers based on the production of free radicals is also discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Hematoporphyrin; Fricke dosimeter; Free radicals.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200006

Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials
Provedor de dados: 52
Autores: Revin,Victor Vasilevich; Kostina,Elena Gennadievna; Revina,Nadezhda Victorovna; Shutova,Vitalina Victorovna.
ABSTRACT Using the classic biotechnological methods, the dependence of A. vinelandii D-05 culture alginate production from the media carbon and nitrogen content was investigated. The maximal alginate production was observed during cultivation bacterium in the medium with 2 to 4% of sucrose, but the maximal growth was found in the medium with 4% glucose. It was found that for the alginate production the optimal nitrogen contents could take from 0.05% yeast extract (carbon: nitrogen ratio 168:1). For the first time we demonstrated possibility the A. vinelandii growth during the cultivation in a medium with molasses (a by-product of sugar production) and the significant polysaccharide production (16.6 g/l) was obtained. It was established, that A. vinelandii...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azotobacter vinelandii; Alginate; Nutrient medium; Carbon: nitrogen ratio; Biocomposite.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100407

Effect of ascorbic acid on food preference and consumption in captive capybaras (Hydrochoerus hydrochaeris)
Provedor de dados: 52
Autores: Alvarez,Martín Roberto; Kravetz,Fernando Osvaldo.
The aim of this work was to study the short and long term influences of ascorbic acid on Hydrochoerus hydrochaeris, if supplemented in their food. No differences regarding food intake were observed in the absence of vitamin C. On a daily basis, animals accustomed to food containing ascorbic acid prefered to continue eating the same food. Food preference in capybaras did not seem to depend on ascorbic acid availability; however, when accustomed to vitamin C, capybaras might recognize and select ascorbic acid in subsequent food. Mechanisms allowing capybaras to "evaluate" the nutritional quality of their food are discussed, as well as the adaptive value of this behavior in free ranging populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin C; Hydrochoeridae; Ecophysiology; Foraging; Nutrition; Feeding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300011

The role of electron-emitting radiopharmaceuticals in the palliative treatment of metastatic bone pain and for radiosynovectomy: applications of conversion electron emitter Tin-117m
Provedor de dados: 52
Autores: Srivastava,Suresh C.
A variety of radionuclides continue to be investigated and/or clinically used for different therapeutic applications in nuclear medicine. The choice of a particular radionuclide with regard to appropriate emissions, linear energy transfer (LET), and physical half-life, etc., is dictated to a large extent by the character of the disease (e.g., solid tumor or metastatic disease), and by the carrier to selectively transport the radionuclide to the desired site. An impressive body of information has appeared in the recent literature that addresses many of these considerations. This article summarizes and discusses the role of high-LET electron emitters and their advantage in the treatment of cancer or for other disorders in specific situations. Areas such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radionuclide therapy; Conversion electron emitters; Bone pain palliation; Radiosynovectomy; Tin-117m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600007

Physiological aspects of sun and shade leaves of Lithraea molleoides (Vell.) Engl. (Anacardiaceae)
Provedor de dados: 52
Autores: Dias,Jaqueline; Pimenta,José Antonio; Medri,Moacyr Eurípedes; Boeger,Maria Regina Torres; Freitas,Claudinei Toledo de.
The aim of this work was to compare the physiological parameters of sun and shade leaves of a specimen of L. molleoides. The higher-positional leaves, classified as sun leaves, presented similar photosynthetic rate, lower chlorophyill contents (a, b and total), same a chlorophyll /b chlrorophyll rate, lower transpiratory rate, same stomatal conductance and intercellular concentration of CO2 as the lower-positional leaves, classified as shade leaves. Nutrient concentration, except for Ca and Mg, was the same for both sun and shade leaves.The physiological parameter responses indicated that although receiving lower light intensity, the shade leaves had the same capacity to grow and develop as the sun leaves.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photosynthesis; Sun leaves; Shade leaves; Light intensity; Chlorophyll; Lithraea molleoides.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100011

Allelopathic potential of orsellinic acid derivatives
Provedor de dados: 52
Autores: Peres,Marize Terezinha Lopes Pereira; Mapeli,Ana Maria; Faccenda,Odival; Gomes,Alcir Teixeira; Honda,Neli Kika.
Environmental degradation caused by the excessive use of pesticides has motivated studies aimed at the discovery of new formulations for pest control. The bioassays carried out under laboratory conditions for the present study involved the germination and seedling growth of Lactuca sativa cv. Grand Rapids and Allium cepa cv. Baia Periforme treated with lecanoric acid or one of seven esters obtained from the alcoholysis of the acid. Each substance was tested at concentrations of 10-3, 10-5, 10-7 M, and 10-9 M. The results revealed that, except for lecanoric acid and methyl orsellinate, all other compounds exhibited significant differences in their action when compared with the controls. Also, A. cepa was more sensitive to the compounds assayed, at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allelopathy; Germination; Growth; Lactuca sativa; Allium cepa; Orsellinates.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400027

A dorsal fold in Gymnura micrura (Bloch and Scheneider, 1801) (Chondrichthyes: Gymnuridae)
Provedor de dados: 52
Autores: Nunes,Jorge Luiz Silva; Piorski,Nivaldo Magalhães.
This paper reports a dorsal fold which is a membranous structure located on the tail of two juvenile butterfly rays, Gymnura micrura (Bloch &amp;amp; Scheneider, 1801), caught through artisanal fishery in the shallow waters of Maranhão State (Brazil).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elasmobranchii; Rays; Teratogeny; South America; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200027

Erratum: Identification of a fat-soluble fraction of Sparganium stoloniferum that inhibits cervical cancer HeLa cells
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300901

Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
Provedor de dados: 52
Autores: Brandão,Aline Dias; Viccini,Lyderson Facio; Recco-Pimentel,Shirlei Maria.
Since previous cytogenetic reports of Aloysia have only described the meiotic behavior and chromosomal number of some species, the aim of this work was to provide detailed cytogenetic description of Aloysia virgata that would contribute to the understanding of the taxonomical organization of the Verbenaceae. Aloysia virgata had a karyotype with 2n = 36 metacentric chromosomes, all with similar size. The large amount of heterochromatin seen after Giemsa staining was confirmed by C-banding. Four nucleolar organizer regions (NORs) were detected with an rDNA 45S probe in two homologous pairs and two sites of 5S rDNA located on one chromosomal pair were detected by fluorescence in situ hybridization. The interphase nucleus was classified as semi-reticulate....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Cytogenetic characterization; FISH; Aloysia; Verbenaceae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400013

A biological treatment technique for wool textile
Provedor de dados: 52
Autores: Xiao-Wei,Yu; Guan,Wen-Jun; Yong-Quan,Li; Ting-Jing,Guo; Ji-Dong,Zhou.
A biological treatment technique for wool textile was carried out by enzymes degradation coupled with H2O2 oxidation. The results demonstrated that the technique had ideal effects on wool textile such as better softness, plump and less loss of bursting stress. Because of mild reaction conditions, less textile damage and less environmental pollution, this technique for wool textile treatment could have promising prospect.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wool textile decrement; Oxidation pretreatment; Enzyme degradation; L86 enzymes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600001

Helicobacter pylori Infection is a Significant Factor Risk for Hyperhomocysteinemia in the Patients with Coronary Artery Disease
Provedor de dados: 52
Autores: Fallah,Soudabeh; Moradi,Nariman; Reza,Fadaei; Reza,Ahmadi; Azardokht,Tabatabei; Seifi,Morteza.
ABSTRACT This work aimed to determine whether seropositivity to Helicobacter pylori infection was an independent risk factor for hyperhomocysteinemia patients with cardiovascular disease. The H. pylori IgG, IgA and homocystein levels in 96 patients with cardiovascular disease and 64 participants free of cardiovascular disease as control subjects were determined by ELISA assay. The results showed that seropositivity to H. pylori IgG and IgA levels of coronary artery disease (CAD)patients was significantly higher than the controls and CAD patients with H. pylori IgG and IgA negative antibodies. A significant correlation was found between the seropositivity to H. pylori IgG and homocysteine levels of CAD patients in comparison with the controls and CAD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular disease; ELISA assay; Homocysteine; Helicobacter pylori.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100304

Biodistribution of 99Mo in rats
Provedor de dados: 52
Autores: Souza,Raphael Sancho Sisley de; Ribeiro,Bianca da Silva; Dantas,Ana Letícia Almeida; Bernardo Filho,Mario; Dantas,Bernardo Maranhão.
The modification of 99Mo standard metabolism in the presence of MDP would alter the dosimetry of this radionuclide in nuclear medicine patients. Therefore, the objective of this work is to evaluate the influence of MDP in the biodistribution of 99Mo. Wistar rats were divided in two groups of six animals, being inoculated respectively 99Molibdate and 99Mo+MDP via plex ocular. The biodistribution study was carried out after 10 and 120 minutes respectively. The organs were counted with a NaI(Tl) detector. The uptake values did not present significant differences among the groups. An in vitro study through planar chromatography was carried out to determine the affinity between molybdenum and MDP. The results show that 99Mo has low affinity both to propanone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wistar; Molybdenum; Nuclear Medicine.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700029

Antimicrobial activity of lactic acid bacteria and the spectrum of their biopeptides against spoiling germs in foods
Provedor de dados: 52
Autores: Djadouni,Fatima; Kihal,Mebrouk.
The objective of this study was to isolate LAB from dairy, meat products and agro-industrial wastes and to investigate their antagonist activity. A total of 141 isolates were screened for the inhibitory effect on ten indicator strains in the agar spot test. Results showed that strain LBbb0141 contained antimicrobial compound with wide spectrum that inhibited the growth of ten indicator Gram-positive and Gram-negative strains. The bacteriocin activity attained its maximum value using the MRS agar at initial pH 7.5 and 30°C incubation temperature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacilli; Enterocoquess; Leuconostoc sp; Bacteriocins activities; Indicators microorganisms; Listeria innovii; Growth media; PH; Temperature.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300015

The effect of low salinity on teredinids
Provedor de dados: 52
Autores: Barreto,Cristine C.; Junqueira,Andrea O. R.; Silva,Sérgio Henrique G. da.
Teredinids survival in low salinities was determined in aquaria. Panels previously immersed in Ponta de Leste, Ilha Grande Bay, Rio de Janeiro, were transferred to aquaria with salinities ranging from 5.0 to 15.0 Practical Salinity Unities (PSU). The waters of Ilha Grande Bay receive a large amount of wood from the marginal rainforest vegetation, being suitable for the development of teredinids. The coastal areas of the bay are subjected to wide salinity fluctuations due to strong and sudden tropical storms. Eleven different species of teredinids were found in the panels. The critical salinity concentration for the survival of the two most common species was 11.93 PSU for Lyrodus floridanus and 12.90 PSU for Teredo furcifera. The effect of time on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaria; Critical salinity; Teredinidae; Rio de Janeiro; Wood-borers.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400008

Growth and Immune Response of Pangasius hypophthalmus Fed Diets Containing Seaweed Extracts as Immunostimulant
Provedor de dados: 52
Autores: Baleta,Francis Nuestro; Bolaños,Jonathan Mallillin.
Abstract Growth and immune response of Pangasius hypophthalmus were evaluated after feeding the fish with diets containing hot-water extracts (HWE) of Sargassum oligocystum as immunostimulant at 100, 300, and 500 mg kg-1 diet. Basal diet for P. hypophthalmus served as the control. The experimental diets were administered for 12 weeks. At the end of the feeding experiment, growth and haematological profile of fish were evaluated. Result showed that final weight, weight gain, daily growth rate and feed conversion ratio were significantly increased in the fish that received 300 and 500 mg kg-1 HWE of S. oligocystum. Evaluation of the haematological profile showed that white blood cells red blood cells, hemoglobin, hematocrit and platelet of P. hypophthalmus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth performance; Hot water extract; Pangasius hypophthalmus; Sargassum oligocystum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100432

A new mini box corer for sampling muddy bottoms in antarctic shallow waters
Provedor de dados: 52
Autores: Echeverría,Carlos Alejandro; Lavrado,Helena Passeri; Campos,Lúcia; Paiva,Paulo Cesar de.
A new Mini Box Corer (MBC-GEAMB) was developed for bottom sampling in Antarctic shallow waters down to 100 m depth from a small vessel. It consists of a detachable stainless steel box with a total sampling area of 0.0625 m², and a closing arm with a detachable blade without an external frame. MBC allowed stratified bottom sampling and good quality samples comparable to those obtained through diving. A comparison between the MBC-GEAMB and a 0.056 m²van Veen grab (VV) was undertaken for the benthic macrofaunal composition in Admiralty Bay, King George Island (Antarctica). MBC and VV samples were taken from three depths (20, 30 and 60m) in two sites. Total densities sampled with the MBC were up to 10 times higher than those obtained with van Veen grab. VV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mini box-corer; Antarctica; Benthos; Sampling.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300015

Preventive Effects of Ellagic Acid on Nucleus Tractus Solitarius Electrical Activity and Oxidative Stress Altered by Cerebral Global Ischemia/Reperfusion in Rat
Provedor de dados: 52
Autores: Nejad,Khojasteh Hoseiny; Sarkaki,Alireza; Dianat,Mahin; Farbood,Yaghoob; Badavi,Mohammad; Gharib-Naseri,Mohammad Kazem.
ABSTRACT Cerebral ischemia commonly occurs when the blood flow to the entire brain or some part of the brain is disrupted. Global cerebral ischemia attenuates the nucleus tractus solitaries (NTS) EEG rhythm, increases the free radicals production and brain inflammation. Ellagic acid (EA) has antioxidative and anti-inflammatory effects against neural damages. The aim of this study was to evaluate the role of ellagic acid on EEG power in the global cerebral ischemia.Rats were divided into four groups: SO (sham) received normal saline, EA+SO, I/R (normal saline + ischemia/reperfusion), and EA + I/R. EA (100 mg/kg, dissolved in normal saline) or normal saline was administered orally (gavage) for 10 days. Animal underwent to 20 minutes of ischemia followed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ellagic acid; EEG; Antioxidant; Cerebral ischemia/reperfusion; Rat.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100446

Micropropagation of an elite F1 watermelon (Citrullus lanatus) hybrid from the shoot tip of field grown plants
Provedor de dados: 52
Autores: Khalekuzzaman,Mohammad; Khatun,Marufa; Rashid,Mohammad Harunur; Sheikh,Mominul Islam; Sharmin,Shamima Akhtar; Alam,Iftekhar.
The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA). Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA). The shoots produced more roots with increasing number of subcultures. About...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro; Watermelon; Micropropagation; Hybrid vigor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300002

Validation of a PCR Assay for Chlamydophila abortus rRNA gene detection in a murine model
Provedor de dados: 52
Autores: Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Negrão,Fábio Juliano; Lunardi,Michele; Freitas,Julio Cesar de.
Chlamydophila abortus (C. abortus) is associated with reproductive problems in cattle, sheep, and goats. Diagnosis of C. abortus using embryonated chicken eggs or immortalized cell lines has a very low sensitivity. Polymerase chain reaction (PCR) assays have been used to detect C. abortus infection in clinical specimens and organ fragments, such as placenta, fetal organs, vaginal secretions, and semen. The aim of this study was to develop a PCR assay for the amplification of an 856-bp fragment of the rRNA gene of the Chlamydiaceae family. The PCR assay was evaluated using organs from 15 mice experimentally infected with the S26/3 reference strain of C. abortus. The results of the rRNA PCR were compared to the results from another PCR system (Omp2 PCR) that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Ovine; Caprine; Reproductive failures; Chlamydophila abortus; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700014

Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens
Provedor de dados: 52
Autores: Mittal,Jitendra; Singh,Abhijeet; Batra,Amla; Sharma,Madan Mohan.
The present study was conducted to explore the hidden potential of natural products synthesized in the medicinal plant Tinosporacordifolia. This plantis prioritized by National Medicinal Plant Board, New Delhi. Leaf and inter nodal segments were inoculated on MS Medium fortified with IBA (1.0 mg/L) produced callus after four weeks. The calli were brown due to phenolic substance secreted by the explant. This problem was overcome by using adjuvant PVP (0.1%). Further, secondary metabolites were isolated from callus and field leaf through soxhlet extractor and fractionated by using column chromatography. The antibacterial activity of these fractioned extracts from Tinosporacordifolia callus and leaf were seen against multi drug resistance bacteria viz.,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Callus; Natural products; PVP; Antimicrobial activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100406

Genetic structure of Brazilian populations of white mouth croaker Micropogonias furnieri (Perciformes : Sciaenidae)
Provedor de dados: 52
Autores: Puchnick-Legat,Angela; Levy,José Alberto.
Mitochondrial (mt) DNA population structure was examined by using restriction fragment length polymorphism analysis (RFLP) among 149 white mouth croakers (Micropogonias furnieri) sampled from six locations in the Brazilian coast. Heterogeneity tests revealed no differentiation in mtDNA haplotype frequencies within the region between 23°S and 34°S (P=0.263), but significant heterogeneity was detected between north and south of 23°S (P&lt;0.003). Analysis of molecular variance (AMOVA) indicated a low F ST value (0.008, P=0.180) among south-central localities (23-34°S), but a significant level of population subdivision (F ST = 0.322, P&lt; 0.003) between north and south-central localities. UPGMA analysis of mtDNA sequence divergence revealed differentiation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic population structure; Mitochondrial DNA; RFLP; Micropogonias furnieri.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400011

Plant breeding in the turn of the millennium
Provedor de dados: 52
Autores: Borém,Aluízio; Milach,Sandra Cristina Kothe.
The transition from hunting and gathering to farming happened about 10,000 years ago, independently and diffusely in several places in the world. Plant breeders were responsible for genetic progress in a number of crop species. It included hybrids, the introgression of wild species genes and also the Green Revolution, which started in the 1960's with the cereals. The varieties developed by breeding, along with the use of new crop technology (fertilization, soil tillage, etc.) changed the status of some countries from importers to exporters of food. In the turn of the millennium,, plant breeding, faces new challenges in a globalized world, but it has new tools to deal with them. Notwithstanding the present contributions of plant breeding and crop...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant breeding; Perspectives; Interespecific biotechnological vulnerability; Genetic gridlock; 21st Century; Partnerships.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300001

Isolation and characterization by asymmetric PCR of the ENDO1 gene for glucan endo-1,3-β-D-glucosidase in Phytophthora cinnamomi associated with the ink disease of Castanea sativa Mill
Provedor de dados: 52
Autores: Meirinho,Sofia; Carvalho,Marisa; Dominguez,Ángel; Choupina,Altino.
Ink disease is one of the most destructive diseases in Castanea sativa. The most common symptoms are root necrosies and a reduction in root growth, which invariably lead to the death of the trees. Phytophthora cinnamomi is an oomycete associated with this disease whose life cycle develops integrally in the soil. In the present work, was a fragment with 1231bp of the glucan endo-1,3-β-D-glucosidase gene obtained by amplification, using conserved primers and the full-length gene sequence by flanking this known sequence by asymmetric PCR. This fragment was obtained from genomic DNA of Phytophthora cinnamomi isolated in the European Regions of Castilla-Leon (Spain) and Trás-os-Montes (Portugal) and associated with the ink disease of Castanea sativa Mill.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytophthora cinnamomi glucan endo-1; 3-β-D-glucosidase asymmetric PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300003

Efficacy of modified atmosphere packaging to control Sitophilus spp. in organic maize grain
Provedor de dados: 52
Autores: Carli,Marcelo De; Bresolin,Bruna; Noreña,Caciano Pelayo Zapata; Lorini,Irineu; Brandelli,Adriano.
The effect of modified atmosphere packaging on the mortality of Sitophilus spp. in organic maize grain was studied. Maize grains were packed with five different atmospheres consisting of 20% O2, or 20, 40, 60, 80% CO2 with remaining N2. The packages were stored for 30 days at 26°C. CO2 and O2 concentrations were monitored inside the packages during the storage. The moisture content, titratable acidity and pH were analysed on the first and thirtieth day. At 5, 15 and 30 days of storage no significant differences were observed in the percentages of dead insects when CO2 was used. The results of progeny indicated that from the fifth day the number of emerging insects was low at 20, 60 and 80% CO2. Complete inhibition of the insects was achieved with 30 days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbon dioxide; Modified atmosphere; Insect mortality.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600024

The effect of phosphoethanolamine intake on mortality and macrophage activity in mice with solid ehrlich tumors
Provedor de dados: 52
Autores: Arruda,Maria Sueli Parreira de; Correa,Mara Adriana; Venturini,James; Félix,Maira Camila; Rosis,Ana Maria Bormio De; Galhiane,Mário Sérgio; Chierice,Gilberto Orivaldo; Costa,Maria Renata Sales Nogueira.
The aim of the present study was to examine the effect of a diet rich in synthetic PEtn on the metabolism macrophages of tumor-bearing mice. The results demonstrated that PEtn increased the animals' survival time. In addition, the treated animals released smaller amounts of hydrogen peroxide (H2O2) and nitric oxide (NO) than the non-treated animals, particularly after day 14. From the results it could be concluded that H2O2 and NO were important in the modulation of neoplastic growth, and pointed to a promising role of PEtn in the control of human neoplasms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphoethanolamine; Ehrlich tumor; Nitric oxide; Hydrogen peroxide; Lipids.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600016

Glutathione production using magnetic fields generated by magnets
Provedor de dados: 52
Autores: Santos,Lucielen Oliveira dos; Gonzales,Tatiane Araujo; Úbeda,Beatriz Torsani; Alegre,Ranulfo Monte.
The objective of this work was to study the production of GSH by Saccharomyces cerevisiae ATCC 7754 in a fermentor (5 L) using a cell recycle system with magnets. The fermentation conditions were 20°C, 500 rpm, 5% (v/v) of inoculum, pHinitial 5, 1.1 vvm aeration and total fermentation time of 72 h. The time of application of MF ranged from 24, 48 or 72 h. In comparison to the control experiment, the best results were obtained with 72 h of application of MF. The cell concentration reached 19.5 g/L and GSH concentration was 271.9 mg/L that corresponded to an increase of 2.63 and 32.1% compared to the control experiment, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione; Magnetic field; Saccharomyces cerevisiae; Batch fermentation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600016

Histological Alterations in Common Carp (Cyprinus carpio Linnaeus, 1758) Gills as Potential Biomarkers for Fungicide Contamination
Provedor de dados: 52
Autores: Stoyanova,Stela; Yancheva,Vesela Slavcheva; Velcheva,Iliana; Uchikova,Ekaterina; Georgieva,Elenka.
ABSTRACTThe present study aimed to investigate the histological alterations in common carp gills caused by a fosetyl-Al and fenamidone based fungicide tested in laboratory conditions at 30, 38 and 50 mg/L concentration. In general, all the tested concentrations activated compensatory-adaptive mechanisms, which caused pathological changes in the fish gills. Results showed different histological alterations in the gill structure, which included lamellar lifting, edema, proliferation of the glandular cells and epithelium, covering the gill filament, fusion and degenerative alterations. Blood circulatory system showed vasodilatation of the secondary lamellae and aneurysms. Overall, there was enhancement of the gill histological changes, which was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histology; Fish; Gills; Pesticides; Contamination; Biomarkers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500757

Development and evaluation of a hydrophilic matrix as a buccoadhesive system containing diclofenac sodium
Provedor de dados: 52
Autores: Wypych,Thaiz Cristina; Andreazza,Itamar Francisco.
The aim of this work was to study the development and evaluation of a hydrolphilic matrix as a buccoadhesive system containing diclofenac sodium. Eleven formulations were prepared containing the following bioadhesive polymers: hydroxylpropylmethylcellulose, polycarbophil, guar gum and xanthan gum individually and in combination. All the formulations were evaluated for the swelling index, adhesive index, and the time of adhesive and drug release profile (%). The results showed that the formulations that presented the most swelling index were the F3 (PAA/GX) and F6 (GG/GX). The smaller index swelling was for F1 (PAA/CM) and F10 (HPCMC/CM). The F4 (PAA/HPMC) formulation presented the best adhesive index and F10 (HPMC/CM) the worst. F1 (PAA/CM) was the best...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diclofenac sodium; Buccoadhesive systems; Guar gum; Xantan gum; Hydroxypropylmethyl cellulose; Polycarbophil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500006

Some Hematological and Non-Specific Immune Responses of Rosehip (Rosa canina) - Fed Russian Sturgeon (Acipenser gueldenstaedtii Brandt &amp;amp; Ratzeburg, 1833) to Mycobacterium salmoniphilum
Provedor de dados: 52
Autores: Duman,Selçuk; Şahan,Aysel.
ABSTRACT In the study, the immunostimulant effects of rosehip (Rosa canina) on the health indicator hematological and non-specific immune parameters of Mycobacterium salmoniphilum-infected sturgeon were investigated. The rosehip was applied in the ratios of R5 (5%), R10 (10%), and R15 (15%) in three repetitions. After a 35-day feeding period, the fish were infected with M. salmoniphilum and analyzed after day 7, when symptoms were observed at elevated levels. Erythrocyte, erythrocyte indices, hemoglobin, hematocrit, leukocyte levels and the non-specific immune parameters including lymphocyte, monocyte, eosinophil, neutrophil, cytokines, phagocytic activity levels were comparatively examined using positive and negative control groups. The evaluations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Russian Sturgeon; Rosehip; Mycobacterium salmoniphilum; Hematological Parameters; Immunological Parameters.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100322

Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)
Provedor de dados: 52
Autores: Machado,Alessandra Cury; Sartori,Patrícia Fernandes; Damante,Carla Andreotti; Dokkedal,Anne Lígia; Oliveira,Rodrigo Cardoso de.
ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p&lt;0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal Plant; Fibroblast; Myracrodruon urundeuva; "aroeira"; "aroeira-do-sertão".
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100301

Length-weight relationship and condition factor of the mangrove crab Ucides cordatus (Linnaeus, 1763) (Crustacea, Brachyura, Ucididae)
Provedor de dados: 52
Autores: Pinheiro,Marcelo Antonio Amaro; Fiscarelli,Ana Gláucia.
The relationship of wet weight (WT) to cephalothorax width (CW) and temporal and seasonal variations in the condition factor were analyzed for each sex of the mangrove crab Ucides cordatus, using specimens collected monthly from September 1998 through September 2000, at Iguape, state of São Paulo. The WT/CW relationship, determined by the regression analysis and the condition factor were evaluated individually, monthly and seasonally, for each sex. The WT/CW relationship indicated isometric growth in males and negative allometric growth in females. Body weight was higher in males than in females of equivalent size and this difference was associated with the males' faster growth and heavier chelipeds. On the other hand, the means for condition factor were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fattening; Mangrove crab; Mangrove forest; Ucides cordatus; Weight increase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200017

Evaluation of CO2 Biofixation and Biodiesel Production by Spirulina (Arthospira) Cultivated In Air-Lift Photobioreactor
Provedor de dados: 52
Autores: Morais,Etiele Greque de; Cassuriaga,Ana Paula Aguiar; Callejas,Nicolás; Martinez,Natalia; Vieitez,Ignacio; Jachmanián,Iván; Santos,Lucielen Oliveira; Morais,Michele Greque de; Costa,Jorge Alberto Vieira.
ABSTRACT Spirulina is a microalgal genre that has the capacity to produce various bioproducts with applications in several areas including the energy sector. The study aimed to assess the ability of CO2 biofixation, biodiesel and other biocompounds production by Spirulina sp LEB 18 cultured in air-lift photobioreactor. The microalgae presented a rich macronutrient composition: protein (47.3%), carbohydrates (13.4%) and a high lipid content (32.7%) in a media with nitrogen reduction, CO2 using air-lift photobiorector. Furthermore, 160 mg.L.d-1 of CO2 was biofixed, generating a maximum biomass yield of 0.02 g.L.d-1. The lipids evaluated for biodiesel production presented a theoretical yield of 19.8% for in situ transesterification and 47.9% for conventional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofuels; Carbon dioxide; Microalgae; Photobiorefinary; Biocompounds.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100703

Heterotrophic succession in carrion arthropod assemblages
Provedor de dados: 52
Autores: Moura,Mauricio Osvaldo; Monteiro-Filho,Emygdio Leite de Araújo; Carvalho,Claudio José Barros de.
Investigations were conducted on carrion attendant insects succession at two contrasting sites to determine if it was a discrete process or not. The visitation pattern of species revealed that saprophagous and predator species had complementary patterns of arrival and that percentage of reoccurring taxa ranged between 40 and 46%, except during winter when it reached 96%. The forested site always presented a higher richness than urban site, and at both the sites the tendency of species increase showed an exponential growth and then a decline. The arriving pattern was clumped in the earlier decompositional stages. At both the sites no clear boundaries were found between the decomposition stages, thus suggesting a continuous process and also suggesting that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carrion Community; Facilitation; Heterotrophic Succession; Insect Succession.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300018

Phenolic Content and Biomolecule Oxidation Protective Activity of Globularia alypum Extracts
Provedor de dados: 52
Autores: Bouriche,Hamama; Kada,Seoussen; Senator,Abderrahmane; demirtas,Ibrahim; Ozen,Tevfik; Toptanci,Bircan Çeken; Kizil,Goksel; Kizil,Murat.
ABSTRACT The protective activity of methanolic (Met E) and aqueous (Aq E) extracts of Globularia alypum L. (G. alypum) against DNA, lipid and protein oxidative damage was investigated. Moreover, the scavenging, chelating, and reducing power activities of the extracts were also evaluated. Phytochemical analysis was performed to determine phenolic compounds. Results showed that Met E and Aq E were rich in phenolic compounds, and were able to scavenge DPPH˙ with IC50 values of 48.61 µg/mL and 51.97 µg/mL, respectively. In addition, both extracts were able to chelate ferrous ions. At 300 μg/mL, the chelating activity was 97.53% and 91.02%, respectively. The reducing power of these extracts was also remarkable and concentration dependent. At 100 µg/mL, both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; DNA damage; Globularia alypum; Lipid peroxidation; Protein oxidation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100422

Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures
Provedor de dados: 52
Autores: Steiner,Neusa; Vieira,Felipe do Nascimento; Maldonado,Sara; Guerra,Miguel Pedro.
The aim of the present work was to establish in vitro conditions for the induction, stabilization and proliferation of embryogenic cultures of A. angustifolia. Pre-cotyledonary staged zygotic embryos inoculated BM medium supplemented with 5 µM 2,4- D, 2 µM BAP and Kin, and 3% maltose or sucrose resulted in 66.7% induction rate. The rate of induction of embryogenic cultures was affected by the carbon source, as well the multiplication and morphology of the embryogenic cultures. Embryogenic cultures maintained in BM medium with maltose presented bipolar morphology. Globular somatic embryos were obtained BM medium with 9% (PEG) and (9%) maltose. These results could establish an in vitro regenerative protocol towards the conservation and improvement of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell competence; Somatic embryogenesis; Carbohydrate; Conifers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800005

Performance of Soybean Seedlings Upon Nutrient Application by Seed Coating
Provedor de dados: 52
Autores: Rufino,Cassyo de Araújo; Tavares,Lizandro Ciciliano; Martín-Ramos,Pablo; Fernandes-Vieira,Jucilayne; Abreu Júnior,José de Souza; Silva,Fábio Júnior Araújo; Fernandes-Correa,Marciabela; Martín-Gil,Jesús.
ABSTRACT The aim of this study was to evaluate the effect of different seed coatings consisting of various combinations of three nutrients (calcium, magnesium and silicon) on two soybean cultivars (BRS 243 RR and CD 233 RR). Dolomitic limestone and aluminum silicate were chosen as the nutrient sources. Leaf area, plant height, shoots dry matter, crop growth rate, relative growth rate and net assimilation rate were the studied variables, evincing that the seed coating that comprised calcium, magnesium and silicon led to better performance in terms of growth rates 30 days after emergence. Significant differences in the response to the seed coatings were also observed between the two studied soybean genotypes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max (L.) Merr.; Growth rate; Leaf area; Micronutrients; Seed treatment.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100500

Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
Provedor de dados: 52
Autores: Travensolo,Regiane de Fátima; Costa,Maria Vitória Cecchette Gottardi; Carareto-Alves,Lucia Maria; Carrilho,Emanuel; Lemos,Eliana Gertrudes de Macedo.
DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA Microarray; Xylella fastidiosa; Expression; Transcriptome.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300006

Effect of flavonoids morin; quercetin and nicotinic acid on lipid metabolism of rats experimentally fed with triton
Provedor de dados: 52
Autores: Ricardo,Kelly Fabiane Santos; Oliveira,Tânia Toledo de; Nagem,Tanus Jorge; Pinto,Aloisio da Silva; Oliveira,Maria Goreti Almeida; Soares,José Francisco.
Atherosclerosis is a coronary disease where deposition of lipids in the arteries leads to problems of blood circulation. The present work evaluates the action of the flavonoids morin, quercetin and nicotinic acid isolated in association on lipid metabolism in hyperlipidemic rats. Blood serum levels cholesterol, cholesterol-HDL, and triacylglycerids have been analysed following the intraperitoneal administration of the flavonoid compounds dissolved in propyleneglycol by in doses of 5mg/kg body weight. Quercetin presented the largest percentual reduction of cholesterol. The best results for cholesterol-HDL have been obtained with-nicotinic acid alone while morin-nicotinic acid combination showed the best triacylglycerols results. Results showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morin; Quercetin; Nicotinic Acid; Lipid; Cholesterol; Cholesterol-HDL; Triacylglycerols; Rats; Hyperlipidemic.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300007

Classification of Flying Insects with high performance using improved DTW algorithm based on hidden Markov model
Provedor de dados: 52
Autores: Rahuman,S. Arif Abdul; Veerappan,J.; Rajesh,R.V.
ABSTRACT Insects play significant role in the human life. And insects pollinate major food crops consumed in the world. Insect pests consume and destroy major crops in the world. Hence to have control over the disease and pests, researches are going on in the area of entomology using chemical, biological and mechanical approaches. The data relevant to the flying insects often changes over time, and classification of such data is a central issue. And such time series mining tasks along with classification is critical nowadays. Most time series data mining algorithms use similarity search and hence time taken for similarity search is the bottleneck and it does not produce accurate results and also produces very poor performance. In this paper, a novel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Classification dynamic time warping nonlinear median filtering optical sensors running spectrum filtering; Voice activity detection.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300402

Use of Solid Waste from Thermoelectric Plants for the Cultivation of Microalgae
Provedor de dados: 52
Autores: Vaz,Bruna da Silva; Costa,Jorge Alberto Vieira; Morais,Michele Greque.
ABSTRACT The aim of this study was to analyze the influence of solid waste on the cultivation of the microalgae Spirulina sp. LEB 18 and Chlorella fusca LEB 111 with 0, 40, 80 and 120 ppm of mineral coal ash. The addition of the ash did not inhibit the cultivation of microalgae at the tested concentrations, showing that it could be used for the cultivation of these microalgae due to the minerals present in the ash, which might substitute the nutrients needed for their growth.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ashes; Chlorella fusca LEB 111; Microalgae; Mineral coal; Spirulina sp. LEB 18.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100200

Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis
Provedor de dados: 52
Autores: Marques,Maria Elizabeth da Costa; Freitas,Beatriz Tupinambá; Guedes,Tarciana Oliveira; Fernandes,Roberta Pereira Miranda.
The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Castor plant; Cytokinin dehydrogenase; Biochemical characterization; Peroxidase.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600003

Mercury and organic carbon distribution in six lakes from the North of Rio de Janeiro State
Provedor de dados: 52
Autores: Sousa,Wanderson Primo de; Carvalho,Carlos Eduardo Veiga de; Carvalho,Claudia Calasans Veiga de; Suzuki,Marina Satika.
The objectives of the study was to evaluate the mercury contamination in sediments of six lagoons located in the north region of Rio de Janeiro State, trying to relate the Hg concentrations with factors that could influence it's mobility, such as organic carbon. The highest values observed in the superficial sediments in lakes like Campelo (349,6 µg.kg -1) suggested a recent Hg input into the system. The correlation Hg/Organic carbon was positive for most areas, indicating that the organic carbon was a significant parameter for Hg retention in this region. The obtained results suggested that the mercury used as fungicide in agriculture lands and in gold mining areas in the past were probably responsible for the contamination of this environment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Organic carbon; Sediment; Lakes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100019

Sensibilidade antimicrobiana de amostras de Escherichia coli isoladas de leitões com diarréia após o desmame
Provedor de dados: 52
Autores: Brito,Benito Guimarães de; Tagliari,Kelly Cristina.
The main infectious agent responsible by post-weaning diarrhoea in piglets is the Escherichia coli and the therapy by drugs is used for control of this disease. The antimicrobial sensitivity of 66 strains of Escherichia coli isolated from piglets with post-weaning diarrhoea was studied. The results show high resistance at four drugs (streptomycin, sulfonamide, tetracycline and chloramphenicol). Gentamycin and neomycin were the best antimicrobial in the inhibition of strains of Escherichia coli, isolated from piglets with post-weaning diarrhoea, in the southwest of Paraná.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E. coli; Antimicrobial sensibility; Drugs; Piglets; Diarrhoea.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100017

Thermal requirements of Trichogramma pretiosum and T. acacioi (Hym.: Trichogrammatidae), parasitoids of the avocado defoliator Nipteria panacea (Lep.: Geometridae), in eggs of two alternative hosts
Provedor de dados: 52
Autores: Pratissoli,Dirceu; Zanuncio,José Cola; Vianna,Ulysses Rodrigues; Andrade,Josimar Souza; Pinon,Tobias Baruc Moreira; Andrade,Gilberto Santos.
This research studied the thermal requirements of Trichogramma pretiosum and T. acacioi (Hymenoptera: Trichogrammatidae), parasitoids of Nipteria panacea (Lepidoptera: Geometridae), with eggs of the alternative hosts Anagasta kuehniella (Lepidoptera: Pyralidae) and Sitotroga cerealella (Lepidoptera: Gelechiidae) aiming to use these natural enemies in biological control programs of this pest in avocado orchards. T. pretiosum needed 151.83 and 160.04 degree-days, above threshold of 10.70 and 10.75°C, while these values were 158.50 and 155.46 degree-days, above threshold of 10.67 and 10.46°C for T. acacioi when exposed to eggs of A. kuehniella and S. cerealella, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avocado; Alternative host; Biological control; Temperature.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500003

Effects of food deprivation in muscle structure and composition of traíra (Hoplias malabaricus): potential implications on flesh quality
Provedor de dados: 52
Autores: Rios,Flavia Sant'Anna; Donatti,Lucélia; Fernandes,Marisa Narciso; Kalinin,Ana Lucia; Rantin,Francisco Tadeu.
Hoplias malabaricus (traíra) were experimentally starved (0 to 240 days) and subsequently re-fed for 30 days (after 90 and 240-d fasting). Long-term starvation produced minor changes in muscle composition, but shrinkage of muscle fibres was a conspicuous result. The diameter of muscle fibres decreased gradually and the spaces among them increased as the starvation period progressed. After re-feeding, fish prior starved for 90 days presented partial restoration on diameter of muscle fibres. However, the fibres remained shrunken after re-feeding following 240-d fasting. While the lipid content did not change, the protein levels fell after 240-d fasting, but they were restored after re-feeding
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Morphology; Muscle; Proximate composition; Starvation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200025

Structure of the phytoplankton in a water supply system in the State of Pernambuco - Brazil
Provedor de dados: 52
Autores: Moura,Ariadne do Nascimento; Dantas,Ênio Wocyli; Bittencourt-Oliveira,Maria do Carmo.
The aim of this work was to study the phytoplankton community composition at limnetic environment in Pernambuco, Brazil. Samplings were carried out from April/2001 to March/2002. Samples to analyses the biotic variables were taken using a recipient with a large overture, at the subsurface and with a Van Dorn bottle at the bottom. The rainfall data were recorded and the water transparency was used to calculate the light attenuation coefficient, photic zone and the determination of trophic state index. The concentrations of total phosphorus and total nitrogen were determined in a typical dry and rainy month. Equitability, also the, similarity and diversity indexes and the densities and correlation of total densities among depths were calculated. Forty-five...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Structure; Phytoplankton; Water supply system; Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400010

Polyphenols From Cutch Tree (Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity
Provedor de dados: 52
Autores: Kumar,Rakesh; Arora,Rohit; Mahajan,Jyoti; Mahey,Sonia; Arora,Saroj.
ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative stress in present work. Leaves of A. catechu were extracted with 80% methanol to get methanol extract (AME). It was assessed for antioxidant activity using DPPH, ABTS, CUPRAC, ferric ion reducing, superoxide scavenging and peroxyl radical scavenging assays. DNA protective activity was also investigated using plasmid nicking assay. Further, antiproliferative activity was determined using MTT assay in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acacia catechu; Antioxidant; Antiproliferative; UHPLC; MTT.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100316

Main Variables of Brazilian Public Policies on Biomass use and Energy
Provedor de dados: 52
Autores: Biernaski,Izabel; Silva,Christian Luiz.
ABSTRACT Biomass is an important energy source, since it considers wastes discarded by other productive processes as inputs. As such, the determination of the most relevant variables, within an institutional dimension, exerts a significant influence to point out actions that should receive greater attention for the satisfactory implementation and monitoring of national public policies. Therefore, the general objective of this study is to determine, within the institutional dimension of the State, the most relevant variables for the decision making of public policies that consider the biomass source as an alternative to the energy matrix of the Brazilian territory. In order to reach the proposed objective, the method used for this research is qualitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Public policies and biomass; Biomass and energy; Public policies and circular economy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200209

Identification of Primordial Germ Cells: Cytological, Histological and Immunohistochemical Aspects
Provedor de dados: 52
Autores: Yön,Nazan Deniz; Akbulut,Cansu.
Primordial germ cells (PGCs) constitute an embryonic cell type that migrate to gonadal precursors and form the gametes. In many animals, PGCs are set apart from somatic cells early during embryogenesis. These cells migrate to gonadal precursors and then constitute gonads so they are useful models for cell motility studies. They have a highlighted importance for development and reproduction studies. Primordial germ cells have morphological differences from the somatic cells. Structure of these cells can be detected with light and electron microscopy in early development stages. This review describes the morphological, histological, molecular and ultrastructural features of primordial germ cells in different animals and gives an overview for simplified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primordial germ cells; Ultrastructure; Histology; Morphology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200222

Effect of harvest period on foliage production and dry matter distribution in five cassava cultivars during the second plant cycle
Provedor de dados: 52
Autores: Sagrilo,Edvaldo; Vidigal Filho,Pedro Soares; Pequeno,Manoel Genildo; Vidigal,Maria Celeste Gonçalves; Scapim,Carlos Alberto; Kvitschal,Marcus Vinícius; Maia,Rogério Rui; Rimoldi,Fabrício.
The objective of this work was to study the leaf production pattern and dry matter distribution in cassava during the second plant cycle. The completely randomized experimental design with four replications was used, with five cultivars in the main plots and ten harvest times in the sub-plots. Foliage production was affected by plant age, being higher in hot periods. Leaf blades and petioles dry matter content presented a linear increase due to a progressive decrease in the amount of young leaves and ontogenetic factors. The stems provided, temporarily, carbohydrates to the plant re-growth, delaying the availability and use of storage roots dry matter. The dry matter content in the storage roots was lower during the vegetative and higher during rest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta Crantz; Harvesting period; Foliage production; Dry matter distribution.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700019

New protein sources in adults diet for mass-rearing of Anastrepha fraterculus (Diptera:Tephritidae)
Provedor de dados: 52
Autores: Morelli,Renata; Costa,Karen Zamboni; Fagioni,Kenya Martins; Costa,Maria de Lourdes Zamboni; Nascimento,Antônio Souza do; Pimentel,Rodrigo Meirelles de Azevedo; Walder,Julio Marcos Melges.
The aim of this study was to find alternatives to reduce the cost of mass production of the South American fruit fly (A. fraterculus) by looking for locally available products as protein source in the diet of adults to replace the imported product without changing the quality parameters. Two yeast from a Brazilian company were evaluated. The quality parameters showed that the imported hydrolyzed yeast used in the adult diet could be perfectly replaced by the local products tested, with a reduction of over 80% of the cost of the diet. The quality of the produced insects remained the same and there were improvements in some quality parameters such as the volume of eggs produced, number of adults flying and longevity under the stress.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: South American fruit fly; Yeasts; Aminoacids; Insect nutrition; Quality control.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600004

Mesozooplankton of an impacted bay in North Eastern Brazil
Provedor de dados: 52
Autores: Silva,Andréa Pinto; Neumann-Leitão,Sigrid; Schwamborn,Ralf; Gusmão,Lúcia Maria de Oliveira; Silva,Tâmara de Almeida e.
Mesozooplankton abundance and distribution at Suape Bay, Pernambuco, was studied to assess the impacts caused by the construction of an internal port to increase the capacity of the Suape Port Complex. Zooplankton sampling was done at 3 stations during the dry (November-December/1997) and rainy (April-May/1998) seasons. A plankton net with 300 µm mesh size was used. Wet weight plankton biomass ranged from 44 mg.m-3 to 3,638 mg.m-3. Forty-five macrozooplankton taxa were registered. The most abundant was Copepoda. Among copepods, Acartia lilljeborgi, Parvocalanus crassirostris, Oithona hebes, Corycaeus (C.) speciosus and Temora turbinata were most frequent. Minimum abundance was 9 ind.m-3 and maximum was 2,532 ind.m-3. Average species diversity was 2.55...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesozooplankton; Copepoda; Human impacts; Suape Port Complex.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300020

Microphytobenthic primary production, biomass, nutrients and pollutants of Santos Estuary (24ºS, 46º20'W). São Paulo, Brazil
Provedor de dados: 52
Autores: Sousa,Eduinetty Ceci Pereira Moreira de; Tommasi,Luiz Roberto; David,Carlos João.
The present study aimed to evaluate the seasonal variation of microphytobenthic biomass and its primary production in the intertidal zone in Santos Estuary (São Paulo, Brazil). This estuary is on the tropical region and it is regarded as a polluted area. The samples were collected at 3 sites, and environmental parameters, nutrients and heavy metals were analyzed. The primary production was evaluated according to GRφNTVED (1960) method whereas chlorophyll a and phaeopigment a were estimated by TETT et al. (1975) method. Total nitrogen K ranged from 20.0 to 115.0 µg·g-1 while total phosphate ranged from 26.0 to 223.0 µg·g-1. No significant seasonal variation of benthic primary production or chlorophyll a and phaeopigment a were observed. Significant positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass; Primary production; Microphytobenthic; Intertidal; Pollutants; Biomassa; Produção primária; Microfitobentos; Entremarés; Poluentes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100004

Effects of pH, Temperature and Agitation on the Decolourisation of Dyes by Laccase-Containing Enzyme Preparation from Pleurotus sajor-caju
Provedor de dados: 52
Autores: Bettin,Fernanda; Cousseau,Francine; Martins,Kamila; Zaccaria,Simone; Girardi,Viviane; Silveira,Mauricio Moura da; Dillon,Aldo José Pinheiro.
(1) Background: In this study, the effects of different pH values ​​(2.4, 3.2, 4.4 and 5.0), temperatures (30, 35, 40, 45 and 50°C) and agitation (100 rpm) on the enzymatic decolourisation of twenty-two dyes belonging to the chromophore groups anthraquinone, azo and triphenylmethane were assessed. (2) Methods: In all conditions, it was used a crude enzyme broth containing 30 U mL-1 laccases produced by Pleurotus sajor-caju PS-2001 in submerged process. (3) Results: Regarding the effects of pH values, the best results were obtained at pH 3.2 and 30°C, in which bleaching was observed for all dyes evaluated. In assays conducted at different temperatures, highest levels of decolourisation were observed at 35°C and pH 3.2 for nineteen of the dyes assessed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pleurotus sajor-caju; Laccases; Dye decolourisation; Anthraquinone; Azo; Triphenylmethane.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100204

Morphological and physiological photon flux influence under in vitro culture of apple shoots
Provedor de dados: 52
Autores: Zanandrea,Ilisandra; Bacarin,Marcos Antonio; Braga,Eugenia Jacira Bolacel; Bianchi,Valmor João; Peters,José Antonio.
The aim of the present study was to evaluate the growth and development of apple rootstock shoots submitted to different photon flux densities (7, 14, 21 and 60 µmol m-2 s-1) and three culture media. The best photon flux for maximizing growth, number of shoots and leaves was 14 µmol m-2 s-1, which also resulted in the highest values of chloroplastic pigments. On the other hand, the highest photon flux (60 µmol m-2 s-1) decreased significantly these parameters. Medium with reduced sucrose concentration did not affect the analyzed parameters, enabling an efficient multiplication of the cultivar with only 1% of sucrose.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mallus; Apple; Sucrose; Photon flux density; Culture medium.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500005

Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
Provedor de dados: 52
Autores: Vasconcelos,André Belico de; Souza,Patrícia Castanheira de; Varago,Fabiana Cristina; Lagares,Monique de Albuquerque; Santoro,Marcelo Matos.
The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcalorimetry; Glucose; Spermatozoa; Sperm metabolism; Cell viability; Equine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010

An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
Provedor de dados: 52
Autores: Leitão,Carlos André Espolador; Cortelazzo,Angelo Luiz.
The present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump. The equipment was tested using the samples of Rodriguezia venusta (Orchidaceae) buds embedded in paraffin, where half of the samples were submitted to the vacuum by the equipment during the embedding. The material was sectioned with a rotary microtome, obtaining full series of quality sections. The control was hard to section with the microtome, obtaining damaged sections due the air bubbles, making the ribbon formation difficult. These results proved the effectiveness of the equipment, making it a practical, inexpensive and more portable solution for newly established laboratories.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embedding; Paraffin method; Plant anatomy; Technique in microscopy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500018

Effect of host availability and egg load in Trichogramma platneri Nagarkatti (Hymenoptera: Trichogrammatidae) and its consequences on progeny quality
Provedor de dados: 52
Autores: Hohmann,Celso L.; Luck,Robert F.
Experiments were conducted to determine whether Trichogramma platneri Nagarkatti manifested a somatic-gametic trade-off and whether it was affected by host availability and host size. Large wasps produced on Trichoplusia ni Hübner and limited to five or 15 T. ni eggs daily, produced a similar number of progeny as wasps offered hosts from emergence. Similar results were obtained when they were deprived of T. ni eggs for one, three, five, or ten days and then provided with unlimited hosts daily until death. In contrast, small wasps produced from Sitotroga cerealella (Olivier) and deprived of T. ni eggs for one to three days suffered a significant reduction in progeny production. Regardless of size, the egg load of non-ovipositing wasps increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Somatic-gametic trade-off; Reproductive success.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300012

Colorimetric determination of alpha and beta-cyclodextrins and studies on optimization of CGTase production from B. firmus using factorial designs
Provedor de dados: 52
Autores: Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Papp,Juliana; Okiyama,Vivian Mayumi de Eiróz; Andrade,Edicléia Alves de; Marcondes,Aluizio de Abreu; Nascimento,Aguinaldo José do.
Cyclodextrin glycosyltransferase (EC 2.4.1.19, CGTase) production from B. firmus, isolated from soil of Curitiba, PR, was optimized in shake flask using an experimental design approach. The CGTase was produced when the carbon source was starch and beta-CD, but when simple sugars such as glucose, galactose, lactose, sucrose, and maltose were used, there was no enzyme production. CGTase production was the same with either organic nitrogen or inorganic nitrogen source. CGTase activity decreased 2-fold when incubation temperature was increased from 28 to 37 ° C, and decreased 2.1- fold when the initial pH was lowered from 10.3 to 7.4. The colorimetric determinations of alpha - and beta -CD were analyzed as a non-linear relationship and the equilibrium constant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Cyclodextrins.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600001

Prediction of Hevea progeny performance in the presence of genotype-environment interaction
Provedor de dados: 52
Autores: Gonçalves,Paulo de Souza; Moraes,Mário Luiz Teixeira de; Silva,Marcelo de Almeida; Gouvêa,Lígia Regina Lima; Aguiar,Adriano Tosoni da Eira; Costa,Reginaldo Brito da.
Twenty two open-pollinated Hevea progenies from different parental clones of the Asian origin were tested at five sites in the Northwestern São Paulo State Brazil to investigate the progeny girth growth, rubber yield, bark thickness and plant height. Except for the rubber yield, the analysis of variance indicated highly significant (p&lt;0.01) genotype x environment interaction and heterogeneity of regressions among the progenies. However, the regression stability analysis identified only a few interacting progenies which had regression coefficients significantly different from the expected value of one. The linear regressions of the progeny mean performance at each test on an environmental index (mean of all the progenies in each test) showed the general...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Stability; Adaptability; Environmental index; Linear regression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100004

Brown Stink Bug Mortality by Seed Extracts of Tephrosia Vogelii Containing Deguelin and Tephrosin
Provedor de dados: 52
Autores: Mikami,Adriana Yatie; Ventura,Maurício Ursi; Andrei,Cesar Cornelio.
ABSTRACT Extracts of the seeds of Tephrosia vogelii Hook. f. were studied in relation to its chemical composition and toxicity to the brown stink bug Euschistus heros (F.). The extracts were obtained in ethyl acetate and ethanol in the sequence according to the polar nature of the solvents. Extracts were sprayed in concentration of 1.0, 2.5, 5.0, 7.5 and 10% on third-instars nymphs and adults, and mortality was recorded. Presence two rotenoids in ethyl acetate was detected, with analyzed with gas chromatography-mass spectrometry (GC-MS). Crude fraction analyses confirmed the presence of these rotenoids (tephrosin - 2.71% in ethyl acetate and 3.66% in methanol; and deguelin - 10.46% in ethyl acetate and 1.22% in methanol) and three other rotenoids in small...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euschistus heros; Rotenoid; Botanical insecticide; Glycine max; Soybeans.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100325

Fuzzy Based Mobility Management in 4G Wireless Networks
Provedor de dados: 52
Autores: Selvi,M Phemina; Sendhilnathan,S.
ABSTRACT Handover is the key procedure in wireless networks to provide required qual ity of service to the users during mobility Handover. As the current scenario of wireless communication comprises of heterogeneity in architecture, application and radio access technologies concentrating on vertical handover becomes important to provide continuous communication and ubiquitous coverage .This paper provides comprehensive survey of the vertical handover, decision technique and parameter used for making proper handover in horizontal and vertical handover . The recent handover schemes are discussed and classified which varies based on concepts and the adopted schemes. A Fuzzy based decision making algorithm is proposed to made the handover decision more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4G; Mobility Management; Heterogeneous Networks; Vertical Handover; Fuzzy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300605

Effect of antioxidants in fresh cut radishes during the cold storage
Provedor de dados: 52
Autores: del Aguila,Juan Saavedra; Sasaki,Fabiana Fumi; Heiffig,Lília Sichmann; Ortega,Edwin Moisés Marcos; Trevisan,Marcos José; Kluge,Ricardo Alfredo.
The objective of this study was to evaluate the efficiency of antioxidants in the cold storage of fresh cut radishes. The shredded roots were submerged in the respective treatments during 3 min (ascorbic acid or citric acid) and stored at 5ºC and 90% RH during 10 days. The radish treated with ascorbic acid showed the higher respiratory rate in the first 4 h after the processing during the storage. The content of total soluble solids (TSS) was significantly higher in the treatment with citric acid. There was an increase in the content of ascorbic acid after two day of storage in the treatments with this antioxidant. The values of lightness (L*) were decreasing along the storage. Citric acid treatments caused strongly red coloration in the minimally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raphanus sativus L.; Enzymatic browning; Respiration; Fresh-cut; Postharvest.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600017

Hydrothermal treatments in the development of isoflavone aglycones in soybean (Glycine max (L.) Merrill) grains
Provedor de dados: 52
Autores: Carrão-Panizzi,Mercedes C.; Goés-Favoni,Silvana Pedroso de; Kikuchi,Akio.
Studies were carried out to enhance the development of isoflavone aglycones in soybean. Grains of two soybean cultivars, BR 36 and IAS 5, 115 and 278 mg/100g of total isoflavone, respectively, were treated hydrothermalyl at 45, 60 and 85° C for 5, 30 and 60 minutes. Pre-treatments of grains at 60° C for 60 minutes allowed a considerable increase of the isoflavone aglycones. Non-treated grains of BR 36 and IAS 5 showed 1.2 mg/100g of genistein, after hydrothermal treatments,which increased to 12 and 53 mg/100g, in each variety, respectively. At higher temperature (85 ° C) there was a decrease of the aglycones due to inactivation of beta-glycosidases. Malonyl compounds were also reduced at higher temperatures. In processing functional soybean foods,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Isoflavones; Glycosides; Aglycones; Temperature.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200010

Microclimate of Atlantic forest fragments: regional and local scale heterogeneity
Provedor de dados: 52
Autores: Ramos,Flavio Nunes; Santos,Flavio Antonio Maës.
The aim of this work was to investigate whether (i) the organisms within different fragments, on regional scale (separated by up to 100 km), could be submitted to similar climatic conditions (rainfall and temperature), and whether (ii) the edge formation in forest fragments could stimulate microclimatic changes (canopy cover, air temperature and soil humidity), on local scale. The organisms within different fragments, on regional scale, were submitted to different climatic conditions, and the edge formation in the forest fragment stimulated microclimatic changes, on local scale, although in a heterogenic way. Not just the distance from the edge influenced the microclimatic differences. Probably, the edge age, location, the matrix structure, as well as, gap...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic forest; Canopy openness; Edges; Fragmentation; Soil moisture; Temperature.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700011

Assessing of biotic integrity of the fish community in a heavily impacted segment of a tropical river in Brazil
Provedor de dados: 52
Autores: Pinto,Benjamin Carvalho Teixeira; Araújo,Francisco Gerson.
A index of biotic integrity (IBI) was applied to assess the ecological health at seven sites of ca. 338 km extension of the Paraíba do Sul river, in bracketing a large urban-industrial complex. The aim was to evaluate the index response to changes in environmental quality during two seasons (winter/dry versus. summer/wet). Eight metrics were selected to IBI in three categories: 1) species richness and habitat composition; 2) indicator species; and 3) trophic structure. The IBI, based on the least impacted regional condition, showed the highest value at the most upstream site (Queluz=km 0) - Acceptable, then decreasing downstream and reaching the lowest record at Volta Redonda (km 125) - Impacted; afterwards there was an increasing quality toward the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish community; IBI; Environmental quality; Habitats; Tropical rivers; Biomonitoring.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300015

In vitro sensitivity of antagonistic Trichoderma atroviride to herbicides
Provedor de dados: 52
Autores: Santoro,Patricia Helena; Cavaguchi,Silvia Akimi; Alexandre,Talita Moretto; Zorzetti,Janaina; Neves,Pedro Manuel Oliveira Janeiro.
Trichoderma atroviride was tested in vitro for its sensitivity to different herbicides. The dosages tested were recommended dosage (RD), half dosage (½RD), and double dosage (2RD). Germination, colony-forming units (CFU), radial growth, and spore production were evaluated. Carfentrazone-ethyl and sulfentrazone inhibited the germination at RD and 2RD. A reduction in the CFU was observed for glufosinate-ammonium, atrazine, carfentrazone-ethyl, diuron + paraquat dichloride, imazapyr, oxyfluorfen, and sulfentrazone at each of the tested dosages. Radial growth was influenced by ametryn, atrazine, carfentrazone-ethyl, oxyfluorfen, and sulfentrazone herbicides, with an 80% reduction of the colonial area. Spore production was affected by carfentrazone-ethyl,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Compatibility; Conservative management; Antagonistic microorganisms; Plant pathogens.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200012

Optimizing dehydration of apples Malus Domestica with fructo-oligosaccharide incorporation
Provedor de dados: 52
Autores: Egea,Mariana Buranelo; Silva,Rui Sérgio Souza Ferreira da; Yamashita,Fábio; Borsato,Dionísio.
The objective of the present study was to study the effect of the variables of the osmotic dehydration process on sliced Fuji apples (Malus domestica) using a 2 x 3² factorial design. The variables studied in the apple slices were the pretreatment (blanching or acidification), the temperatures (30, 45 and 60ºC) and the FOS concentration (40%, 50% and 60% m/v) of the osmotic solution. There was no difference among the pretreatments for the water activity and titratable acidity. The slices pre-treated by the acidification presented less enzymatic browning (greatest luminosity L* value) combined with a greater soluble solid contents (thus, this treatment was selected). Treatments T4 (45ºC and 40% m/v) and T7 (60ºC and 40%m/v), using the acidification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Surface response methodology; Optimization; Functional food; Fructo-oligosaccharide.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500015

Fructans and water suppression on intact and fragmented rhizophores of Vernonia herbacea
Provedor de dados: 52
Autores: Dias-Tagliacozzo,Gláucia M.; Itaya,Nair M.; Carvalho,M. Angela Machado de; Figueiredo-Ribeiro,Rita de Cássia L.; Dietrich,Sonia M. C.
In order to evaluate the involvement of fructans in drought tolerance, experiments were conducted using intact plants and excised rhizophores of Vernonia herbacea. The water content in rhizophores of intact plants was maintained for 30 days when plants were watered every 7 and 15 days, whereas plants remained alive up to 60 days without water. Total fructan, oligo- to polysaccharides ratio and mean molecular mass of polysaccharides increased in these plants indicating depolymerization of median size molecules. In apical fragments of rhizophores kept dry the oligosaccharides increased in relation to polysaccharides one day after excision in treated tissues. This was reflected in the HPLC profile of the oligosaccharides in which the proportion of free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulin; Environmental stresses; Drought; Fructan metabolism; Reserve organs; Asteraceae.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300005

Tissue distribution and deposition pattern of a cellulosic parenchyma-specific protein from cassava roots
Provedor de dados: 52
Autores: Souza,Petrônio A.S.; Gomes-Filho,Enéas; Campos,Francisco A.P.
A protein with a molecular mass of 22kDa was purified from the cellulosic parenchyma of cassava roots. The amino acid composition of the protein was determined and antibodies generated against the purified protein were used to show that the concentration of the protein remains unchanged during root "tuber" formation. By using a tissue printing technique, as well as western blot, it was shown that the cellulosic parenchyma was the only root tissue in which the protein was deposited.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Mandioca; Proteinas vegetais; Cassava; Plant protein.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100001

Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay
Provedor de dados: 52
Autores: Monte,Leonardo Garcia; Leal,Fernanda Munhoz Dos Anjos; Hartwig,Daiane Drawanz; Vasconcellos,Sílvio Arruda; Brihuega,Bibiana; Dellagostin,Odir Antonio; Hartleben,Cláudia Pinho.
The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL32 was produced in eukaryotic expression system (Pichia pastoris) and evaluated the antigen by enzyme-linked immunosorbent assay (ELISA). The yield obtained from the culture supernatant reached 270 mg/L and ELISA showed an accuracy of 95.34%. In summary, the production of rLipL32 using P. pastoris did not impair the antigenic characteristics of this antigen and ensured its use for detecting the leptospiral antibodies in swine sera.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pichia pastoris; LipL32; ELISA; Leptospirosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300007

Anti-obesity Effects of the Administration of Tournefortia paniculata Cham Extract on Wistar Rats Subjected to a Hypercaloric Diet
Provedor de dados: 52
Autores: Simão,Anderson Assaid; Ramos,Vinicius de Oliveira; Corrêa,Angelita Duarte; Sousa,Raimundo Vicente de; Marcussi,Silvana.
The aim of this study was to evaluate the therapeutic and toxicologic effects of the administration of the powered vegetable extract and aqueous extract of Tournefortia paniculata leaves on Wistar rats, subjected to a hypercaloric diet for 42 days. The rats were divided into five groups and were given the following treatments by gavage: T0 (control) - 1.0 mL water day-1; T1 - aqueous extract containing 14 mg phenolic compounds kg-1 rat day-1; T2 - 14 mg quercetin kg-1 rat day-1; T3 - 50 mg powered vegetable extract from T. paniculata leaves (PVE) kg-1 rat day-1 and T4 - 100 mg PVE kg-1 rat day-1. The treatments did not significantly alter the weight, but were effective in reducing liver fat, glucose and serum triglycerides. The treatment T1 reduced food...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Medicinal plants; Pharmacology; Antioxidants; Genotoxicity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400494

Evaluation of the haematology and biochemistry of Clarias gariepinus as biomakers of environmental pollution in Tiga dam, Nigeria
Provedor de dados: 52
Autores: Abalaka,Samson Eneojo.
This study aimed to evaluate the haematological and biochemical changes in Clarias gariepinus as biomarkers of environmental pollution in Tiga dam, Nigeria (wild aquatic environment). Water and fishes were sampled twice, a week apart, from the controlled and the wild aquatic environment. There were no significant (p&amp;gt;0.05) differences between the temperature, pH and dissolved oxygen contents of both aquatic environments. Similarly, there were no significant (p&amp;gt;0.05) changes in the haematological parameters of the reared and wild the C. gariepinus except in their white blood cell counts, which were significantly (p&lt;0.05) higher in wild C. gariepinus. The activities of aspartate aminotransferase, alanine aminotransferase and alkaline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catfish; Serum enzymes; Serum metabolites; Blood values; Aquatic environment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300004

Responses of Neomegalotomus parvus (Hemiptera: Alydidae) to color and male-lured traps
Provedor de dados: 52
Autores: Ventura,Maurício Ursi; Panizzi,Antônio Ricardo.
In 1998, the relative responses of Neomegalotomus parvus (Westwood) (Hemiptera: Alydidae) to colors in traps were assessed in the field. Colors were printed in white alkaline paper and covered with transparent plastic. Printed colors were black, green, yellow, red and blue. Treatments also included white paper and aluminum foil. Green traps captured more bugs than the other traps (except yellow). Yellow traps caught significantly more N. parvus males than blue, black and aluminum traps. White, red and blue traps captured significantly more N. parvus males than black traps. In 1999, the same treatments were used and additionally included the factor presence (X absence) of males in cages in the traps. Four males were confined in the cages together with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Semiochemical; Color responses; Aggregation pheromone.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400005

Characterization of tropical starches modified with potassium permanganate and lactic acid
Provedor de dados: 52
Autores: Takizawa,Fabiano Franco; Silva,Graziela de Oliveira da; Konkel,Francisco Eneias; Demiate,Ivo Mottin.
In the present work some tropical starches were modified by an oxidative chemical treatment with potassium permanganate and lactic acid. The native and modified samples were evaluated by mid-infrared spectroscopy, differential dyeing, pH, expansion power, solubility and swelling power, clarity of the pastes, susceptibility to syneresis, carboxyl content and reducing power. All modified samples presented dark blue color, higher expansion power (except corn starch), carboxyl content and reducing power. The solubility of the modified starch granules was very high at 90ºC. At this temperature, it was not possible to measure their swelling power. The viscographic analysis showed decrease in peak viscosity and higher degree of cooking instability. The principal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical starches; Oxidized starches; FTIR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600012

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis
Provedor de dados: 52
Autores: Batista,Tânia Regna; Mendonça,Evânia Galvão; Pádua,Marlúcia Souza; Stein,Vanessa Cristina; Paiva,Luciano.
ABSTRACT The aim of this study was to induce and analyze embryogenic calli from two types of explants (leaves and meristems) of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Leaves and meristems of plants kept in a nursery were disinfected and inoculated in Petri dishes containing MS culture medium supplemented with different concentrations of the growth regulator dicamba (1.13, 4.52, and 9.04 µM) and without it. At 60 days of culturing, the calli were analyzed by scanning electron microscopy and at 90 days were evaluated by light microscopy in regard to the embryogenic characteristics of the cells. Different type of calli were induced in leaf explants, designated as Type I with light yellow coloring, Type II with dark yellow coloring, and Type III...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomical analyses; Dicamba; Eucalyptus; Somatic embryos; Ultrastructural analyses.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100430

Longissimus dorsi fatty acids composition of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in pasture
Provedor de dados: 52
Autores: Prado,Ivanor Nunes do; Moreira,Fernanda Barros; Matsushita,Makoto; Souza,Nilson Evelázio de.
Studies were carried out to evaluate the fatty acids composition of Longissimus dorsi muscle, with or without fat thickness, of Bos indicus and Bos indicus x Bos taurus crossbred steers finished in millet (Pennisetum americanum L.) or star grass (Cynodon plectostachyus Pilger) pasture systems with mineral or mineral protein supplementation. Neither the treatment nor the genetic group had effect on meat fatty acids composition. The cut had effect on meat fatty acid composition. The meat without fat thickness had 11% of PUFA, 43% of MUFA and 45% of SFA. The meat with fat thickness had 5% of PUFA, 44% of MUFA and 50% of SFA. PUFA/SFA ratio was 0.10 and 0.25 and n-6/n-3 ratio was 0.80 and 1.27 for cuts with and without fat thickness.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; Bos taurus; Fatty acids; Fat; Meat; Pasture.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400015

Effect of indomethacin on the pregnant rat
Provedor de dados: 52
Autores: Damasceno,Débora Cristina; Volpato,Gustavo Tadeu; Ferrari,Cássio; Roldan,Luciana B.; Souza,Maricelma da Silva Soares.
The objective of this study was to evaluate the reproductive performance, liver morphological study and post mortem characteristics of the pregnant Wistar rats treated with indomethacin, a general COX inhibitor. Indomethacin at doses of 0 (control), 0.32, 1.68 and 8.40 mg/kg/day were orally given once daily to each group (n=10) on days 3 and 4 of pregnancy (day 0 = first day of pregnancy = positive vaginal sperm). The animals were euthanized under anesthesia on day 11 of pregnancy, and were carried out necropsy and microorganism culture study. The results showed that the doses of 0.32 and 1.68 mg/kg body weight (the therapeutic dose for humans) of indomethacin caused no embryotoxic or lethal effects. The highest dose (8.40 mg/kg) of indomethacin disturbed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indomethacin; COX; Pregnancy; Rat; Implantation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100010

Cytotoxic Effect on Cancerous Cell Lines by Biologically Synthesized Silver Nanoparticles
Provedor de dados: 52
Autores: Kulandaivelu,Balaji; Gothandam,K M.
The biosynthesis of nanoparticles has been proposed as an environmental friendly and cost effective alternative to chemical and physical methods. Silver nanoparticles are biologically synthesized and characterized were used in the study. The invitro cytotoxic effect of biologically synthesized silver nanoparticles against MCF-7 cancer cell lines were assessed. The cytotoxic effects of the silver nanoparticles could significantly inhibited MCF-7 cancer cell lines proliferation in a time and concentration-dependent manner by MTT assay. Acridine orange, ethidium bromide (AO/EB) dual staining, caspase-3 and DNA fragmentation assays were carried out using various concentrations of silver nanoparticles ranging from 1 to 100 μg/mL. At 100 μg/mL concentration, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver nanoparticle; Nanotechnology; Nanobiotechnology; Nanoparticle; Cytotoxicity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100307

The effect of temperature and body size on filtration rates of Limnoperna fortunei (Bivalvia, Mytilidae) under laboratory conditions
Provedor de dados: 52
Autores: Pestana,Débora; Ostrensky,Antonio; Boeger,Walter Antonio Pereira; Pie,Marcio Roberto.
The golden mussel (Limnoperna fortunei, Mollusca: Bivalvia) is an invasive species that has been causing considerable environmental and economic problems in South America. In the present study, filtration rates of L. fortunei were determined in the laboratory under different temperatures (10, 15, 20, 25, 28, and 30 ºC) and two types of food (Algamac-2000® and the chlorophycean alga Scenedesmus sp.). There was a statistically significant relationship between time and filtration rates in the experiment using Scenedesmus sp., regardless of temperature. However, this pattern was absent in the experiment using Algamac, suggesting that the relationship between filtration rates and temperature might depend on the size of the filtered particles. In addition, there...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Invasive species; Condition index; Scenedesmus sp.; Algamac-2000®; Environmental impact.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100018

Contribution to a macromycete survey of the states of Rio Grande do Sul and Santa Catarina in Brazil
Provedor de dados: 52
Autores: Sobestiansky,Georg.
Collections of macromycetes made in seven municipalities in southern Brazil, viz. six in Rio Grande do Sul and one in Santa Catarina, are listed. They belonged to the Myxomycota (6 spp.), Ascomycota (54 spp.) and Basidiomycota (189 spp.). First records for Brazil could be Battarrea phalloides, Amanita rubescens, Boletus edulis and Mycena filopes, the last three found under exotic Pinus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macromycetes; Rio Grande do Sul; Santa Catarina; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300015

A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
Provedor de dados: 52
Autores: Zanella,Janice; Costa,Sergio Olavo Pinto da; Zacaria,Jucimar; Echeverrigaray,Sergio.
Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acanthamoeba; Monocystic culture; Micromanipulation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001

Arsenic tolerance of Microcystis novacekii (Komárek-Compère, 1974) and its arsenic decontamination potential
Provedor de dados: 52
Autores: Ferreira,Fernanda Aires Guedes; Franco,Maione Wittig; Oliveira,Dirce Maria De; Magalhães,Sérgia Maria Starling; Barbosa,Francisco Antônio Rodrigues.
ABSTRACT Cyanobacteria possess metallic ion interaction properties that should be explored with the aim of recovering arsenic (As) contaminated areas. Contamination of As is an issue of worldwide concern due to the risk of human chronic intoxication and negative environmental health effects. In this study the potential decontamination of As(III) and As(V) using cyanobacteria cultures was assessed. Microcystis novacekii (Komárek-Compere, 1974) showed normal growth in concentrations of As(V) similar to those found in natural environments contaminated with As, demonstrating its resistance to As(V). Growth rates gradually decreased upon exposure to high As(V) concentrations from 600 to 5630 mg.L-1 while As(III) affected growth from 14.7 - 85.7 mg.L-1. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arsenic; Cyanobacteria; Bioaccumulation; Toxicity; Growth rates.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100414

Environmental and subtidal fish assemblage relationships in two different brazilian coastal estuaries
Provedor de dados: 52
Autores: Vendel,Ana Lúcia; Bouchereau,Jean-Luc; Chaves,Paulo de Tarso.
The general structure and organization of the shallow water (&lt;1.5 m) fish assemblages were studied in two southern Brazilian ecosystems, a bay and a lagoon, under different marine or continental influences. The abiotic factors were measured to define the hydrology of the sites and the biotic descriptors were evaluated to characterize the assemblage structures. Transparency, salinity and organic matter were the abiotic factors which best distinguished the sites. A total richness of 69 species was observed. Ecological guilds, density, diversity and evenness indices values were different at each site. The hydrodynamism and geomorphology determined the structure and the organization of the fish assemblages in these shallow waters. It was observed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish biodiversity; Bay; Lagoon; Spartina marsh; Southern Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600016

Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage
Provedor de dados: 52
Autores: Fiorentini,Ângela Maria; Sawitzki,Maristela Cortez; Bertol,Teresinha Marisa; Brod,Fábio Cristiano Angonesi; Pelisser,Márcia Regina; Arisi,Ana Carolina Maisonnave; Sant'Anna,Ernani Sebastião.
Micrococcaceae strains are applied to fermented sausage as starter cultures, where several members of this family are naturally found. The aim of the present work was to isolate and characterize Staphylococcus xylosus from artisanal sausages produced in South Region of Brazil. From 89 isolates presenting catalase positive and coagulase negative activities, 25 strains were selected for phenotypic characterization. Nine strains identified as Staphylococcus xylosus by API-STAPH were evaluated for their nitrate reduction capacity, which showed satisfactory growth of the strains in the presence of nitrite and sodium chloride, demonstrating their potential for use as starter cultures in fermented sausage. The strains were also evaluated through genus and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus xylosus; Ermented sausage; Starter cultures; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300026

Water quality in watershed of the Jaboatão River (Pernambuco, Brazil): a case study
Provedor de dados: 52
Autores: Souza,Antonio Donizetti Gonçalves de; Tundisi,José Galizia.
The purpose of the present work was to evaluate anthropogenic influences on the water quality and to offer a subsidy to the establishment of water quality goals in the Jaboatão River Basin (Pernambuco State, Brazil). Eight sampling points were established and were sampled monthly during one hydrological cycle (March/98-February/99). The following variables were analyzed: temperature, pH, conductivity, chlorine, alkalinity, dissolved oxygen, biochemical oxygen demand, fecal coliforms, nitrate, total phosphorus and total solids. The most critical variables related to water quality objectives were dissolved oxygen, fecal coliforms and total phosphorus. Maps of land use, legally protected areas, area industries, and water withdrawals were utilized in order to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Watershed; Water quality; Environmental management.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400026

Research and Applications of Shop Scheduling Based on Genetic Algorithms
Provedor de dados: 52
Autores: ZHAO,Hang; KONG,Fansen.
ABSTRACT Shop Scheduling is an important factor affecting the efficiency of production, efficient scheduling method and a research and application for optimization technology play an important role for manufacturing enterprises to improve production efficiency, reduce production costs and many other aspects. Existing studies have shown that improved genetic algorithm has solved the limitations that existed in the genetic algorithm, the objective function is able to meet customers' needs for shop scheduling, and the future research should focus on the combination of genetic algorithm with other optimized algorithms. In this paper, in order to overcome the shortcomings of early convergence of genetic algorithm and resolve local minimization problem in search...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shop scheduling; Genetic algorithm; Local minimization; Cyclic search.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200601

Incorporation of n-3 fatty acids by the liver of mice fed linseed oil as a function of feeding duration
Provedor de dados: 52
Autores: Perini,João Angelo Lima; Stevanato,Flávia Braidotti; Visentainer,Jeane Eliete Laguila; Sargi,Sheisa Cyléia; Oliveira,Marcia Machado; Souza,Nilson Evelázio; Matsushita,Makoto; Visentainer,Jesuí Vergílio.
The objective of this study was to evaluate the incorporation of omega-3 fatty acids (n-3 FA) on male Swiss mice livers receiving diets based on linseed oil (LO) for up to 56 days. The mice were sacrificed at 7, 14, 28, 42, 56 days and FA concentration was analyzed by gas chromatography. A total of 13 FA were identified on LO feeds presenting alpha-linolenic acid (LNA) contents of 26.97 %. A total 22 FA were identified in the livers of the mice. Part of the LNA was converted into n-3 FA (20:3n-3, eicosapentaenoic acid-EPA, 22:5n-3, and docosahexaenoic acid-DHA), and some of the LNA was stored in the liver. LNA, EPA and DHA concentrations (mg/g total lipids ) from 0 days for up 56 days increased from 1.29 to 18.90 (LNA), 0.20 to 18.90 (EPA) and 41.26 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyunsaturated fatty acids; Mice; Liver; Linseed oil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200012

Comparison between two selected Saccharomyces cerevisiae strains as fermentation starters in the production of traditional cachaça
Provedor de dados: 52
Autores: Gomes,Fátima de Cássia Oliveira; Araújo,Roberta Amália de Carvalho; Cisalpino,Patrícia Silva; Moreira,Elizabeth Spangler Andrade; Zani,Carlos Leomar; Rosa,Carlos Augusto.
Two Saccharomyces cerevisiae strains were tested as the starter yeasts in a traditional cachaça distillery. The strains used were S. cerevisiae UFMG-A829, isolated from a cachaça fermentation process, and S. cerevisiae K1-V1116, obtained from the wine industry. The permanence of each strain in the fermentation must was determined by RAPD (Random Amplified Polymorphic DNA)-PCR, with primer M13. Both yeast strains were prevalent in the vats for approximately 30 days. Indigenous non-Saccharomyces and indigenous S. cerevisiae strains were isolated in lower counts during the fermentation period. Indigenous S. cerevisiae strains were molecularly distinct when compared to the starter yeasts. The two yeasts appeared promising starter yeasts in the fermentation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cachaça; Saccharomyces cerevisiae; Starter strains; Fermentation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200023

Chromosome number and microsporogenesis in Paspalum maritimum (caespitosa group; gramineae)
Provedor de dados: 52
Autores: Adamowski,Eleniza de Victor; Pagliarini,Maria Suely; Batista,Luiz Alberto Rocha.
Despite of economic importance of the genus Paspalum, little or no cytologic information is available for many species. This is the first report about chromosome number and meiotic behavior for P. maritimum. The three accessions collected in Amapá State (North Region of Brazil) were tetraploid (2n=4x=40) with the chromosomes associating predominantly as bivalents. The low frequency of multivalents suggested that they were segmental allotetraploids. All accessions showed a low rate of meiotic irregularities, and as a consequence the pollen fertility was high. The results suggested that these accessions presented potential for use in a hybridization program.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paspalum maritimum; Chromosome number; Meiotic behavior; Pollen fertility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300009

In Vivo investigation of radiolabeled Bevacizumab in healthy rat tissues
Provedor de dados: 52
Autores: Demir,Ilknur; Muftuler,Fazilet Zumrut Biber; Unak,Perihan; Acar,Cigdem.
In this study, BevMab was conjugated with the bifunctional chelating agent [diethylenetriamine pentaacetic acid (DTPA)] and the product (BevMab-DTPA) was labeled with 99mTc using stannous chloride reducing method. The quality control studies of radiolabeled compound (99mTc-BevMab-DTPA) were done with Thin Layer Radio Chromatography (TLRC) and High Performance Liquid Radio Chromatography (HPLRC) methods (% 95 &lt;) to confirm the labeling efficiency. High radiochemical yield [98.07 % ± 2.17 (n = 13)] was obtained by TLRC method. Biodistribution studies of 99mTc labeled BevMab-DTPA was run on healthy female and male Albino Wistar rats. The distribution figures demonstrated that the radiolabeled compound was eliminated through the kidneys and accumulated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Bevacizumab; 99mTc.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100010

Path analysis under multicollinearity in soybean
Provedor de dados: 52
Autores: Bizeti,Henrique Stoco; Carvalho,Claudio Guilherme Portela de; Souza,José Roberto Pinto de; Destro,Deonisio.
This study aimed to establish the phenotypic correlations among several soybean traits with grain yield in direct and indirect effects using path analysis, and to compare alternative methods for minimizing the adverse effects of multicollinearity in estimating path coefficients. The experiment was conducted in greenhouse in a randomized complete block design with four replications. Nine soybean genotypes belonging to three seed size categories were used. The correlation studies and the path analysis showed that the seed size was not important for increased yield. The number of nodes and plant height at maturity showed significant correlation with grain yield. Using the least square methodology, the results obtained by path analysis under multicollinearity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic breeding; Glycine max; Grain yield; Seed size; Root and canopy traits.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500001

123-I ioflupane (Datscan®) presynaptic nigrostriatal imaging in patients with movement disorders
Provedor de dados: 52
Autores: Soriano Castrejón,Angel; García Vicente,Ana María; Cortés Romera,Montserrat; Cano,Julia Vaamonde; Marina,Sonia Rodado; Poblete García,Victor Manuel; Ruiz Solís,Sebastián; Talavera Rubio,María del Prado.
123-I Ioflupane (Datscan®) presynaptic imaging has been shown to have a significant utility in the assessment of patients with movement disorders 123-I Ioflupane SPECT is able to distinguish between Parkinson’s disease (PD) and other forms of parkinsonism without degeneration of the nigrostriatal pathway, including a common movement disorder such as essential tremor, and to assess disease progression in PD and other neurodegenerative disorders involving the substantia nigra.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 123-I Ioflupane; Dopamine transporter imaging; Movement disorders; Parkinson’s disease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700017

Eggs fertility and biological aspects of the life cycle in natural populations of Simulium (Diptera, Simuliidae)
Provedor de dados: 52
Autores: Petry,Fabiane; Lozovei,Ana Leuch; Chahad-Ehlers,Samira.
Eggs fertility rate and some biological aspects of the immature forms were studied on six Simulium species from ovipositions collected in natural breeding places located in rural area. The fertility was considerably high. For a total of 213 ovipositions containing 74,906 eggs, the average fertility rate was 99.13% with variation from 94 to 100%. The developmental cycle of the immature forms was observed from egg eclosion to adult emergence under temperature from 16.5 to 28ºC. In S. orbitale the life cycle duration was 30 to 32 days, for S. perflavum 32 to 35 days, for S. subnigrum 33 to 39 days, for S. inaequale 41 to 47 days, for S. pertinax 42 to 48 days, and finally, the longest duration was observed for S. distinctum to range 48 to 49 days.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blackfly; Egg fertility; Life-cycle; Oviposition; Simuliidae; Simulium.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600014

Antinociceptive and Anti-inflammatory Activities of Marine Sponges Aplysina Caissara, Haliclona sp. and Dragmacidon Reticulatum
Provedor de dados: 52
Autores: Hort,Mariana Appel; Silva Júnior,Flavio Manoel Rodrigues da; Garcia,Edariane Menestrino; Peraza,Gianni Goulart; Soares,Andrea; Lerner,Clea; Muccillo-Baisch,Ana Luiza.
ABSTRACT Marine sponges are a rich source of bioactive natural products with multiple pharmacological properties. In this study, the anti-inflammatory and antinociceptive effects of extracts obtained from Aplysina caissara, Haliclona sp. and Dragmacidon reticulatum were evaluated by using the writhing test and formalin-induced mouse paw edema model in mice. All extracts were administered via oral pathway in the doses of 60 and 90 mg/kg. In the writhing test the pre-treatment with all sponges resulted in significant inhibition of the acetic acid-induced response, suggesting an antinociceptive effect. The formalin test showed that the extracts from A. caissara, Haliclona sp. and D. reticulatum, in the tested doses, did not affect the first formalin phase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Nociception; Marine sponges.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100433

Spatial and temporal variations of diurnal ichthyofauna on surf-zone of São Francisco do Itabapoana beaches, Rio de Janeiro State, Brazil
Provedor de dados: 52
Autores: Gomes,Marcelo Paes; Cunha,Miguel Siano; Zalmon,Ilana Rosental.
Spatial and temporal variations of diurnal ichthyofauna and the environmental variables influences on its distribution were studied at the surf-zone of three beaches of São Francisco do Itabapoana, northern coast of Rio de Janeiro, Brazil. From August/1999 to August/2000, three beach seine hauls were made monthly, and environmental variables were recorded. A total number of 4,562 fishes (74,155g) were sampled at the three beaches, where estuarine-dependent species prevailed (44%), followed by marine (31%), estuarine (19%) and freshwater species (3%). Species richness, number of individuals and wet weight were significantly higher at Gargaú, followed by Manguinhos and Barra do Itabapoana, respectively. Canonical Correspondence Analysis highlighted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spatial and temporal variations; Community ichthyofauna structure; Surf-zone; Environmental variables; North coast of Rio de Janeiro.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400020

Anticoccidial activity of Curcuma longa L. in broilers
Provedor de dados: 52
Autores: Abbas,Rao Zahid; Iqbal,Zafar; Khan,Muhammad Nisar; Zafar,Muhammad Arif; Zia,Muhammad Anjum.
Comparative efficacy of turmeric (Curcuma longa L.) crude powder and salinomycin sodium on the occurrence of coccidiosis and growth performance of broiler was evaluated. A total of 90, day-old chicks were randomly divided into six groups. From first day onward, ration was supplemented with 1, 2 and 3 % turmeric powder in groups 2, 3 and 4, respectively, group 1 received salinomycin sodium @ 12 g 50 kg-1 feed while groups 5 and 6 were kept as infected un-medicated and uninfected un-medicated controls. First five groups were infected with Eimeria tenella sporulated oocysts @ 1,00,000/chick at the age of 20 days. Body weight gain, feed consumption, feed conversion ratio were investigated throughout the experimental period, and bloody diarrhea and oocysts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcuma longa; Coccidiostat; Eimeria tenella.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100008

Occurrence of Pyemotes sp. on Tuta absoluta (Meyrick)
Provedor de dados: 52
Autores: Oliveira,Carlos Romero Ferreira de; Matos,Cláudia Helena Cysneiros; Hatano,Eduardo.
The aim of this work was to study the population reduction of the tomato leafminer moth, Tuta absoluta (Meyrick), by Pyemotes sp. in the laboratory. The mite became greenish when fed on caterpillars, pupae, and adults of T. absoluta. The caterpillars and adults of T. absoluta were quickly paralyzed by the mite venom. A single T. absoluta could host many Pyemotes sp, physogastric females, which were allowed to grow on the moth. Pyemotes sp. can be a new alternative for the biological control of T. absoluta. However, this possibility must be better understood before it could be recommended, because Pyemotes sp. could also cause dermatitis in the humans.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mite; Parasitism; Tomato leafminer; Biological control.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700003

Alternatives to sowing vegetable type soybeans
Provedor de dados: 52
Autores: Mannfredini,Edcarlos; Destro,Deonisio; Homechin,Martin; Sachsida,Gustavo; Miglioranza,Édison.
Today, soybean crops of the Tamba Kurodaisu cultivar are sown in beds prior to transplantation to the field. This planting system has caused crop failure due to damage to the root system. An experiment to test different sowing alternatives to obtain plantlets for cropping of food type big seeded soybean was set up with the following treatments: sowing in beds; sowing in 130 cm³ newspaper cups; sowing in test tubes of volumes of 30 cm³, 60 cm³ and 70 cm³; sowing in 70 cm³ disposable plastic cups; sowing on 90 cm³ styrofoam trays. A randomized complete block design was used, and the following traits were assessed: germination percentage; number of days to flowering; plant height at flowering; number of days to maturity; plant height at maturity; number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food type soybean; Vegetable soybean; Edamame.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400007

EDITORIAL
Provedor de dados: 52
Autores: Anand,P.; Kumar,B. N. Siva Prasanna.
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300100

Microhabitat occupation by birds in a restinga fragment of Paraná coast, PR, Brazil
Provedor de dados: 52
Autores: Pedroso Junior,Nelson Novaes.
Studies were carried out to characterize microhabitat occupation by birds in a restinga fragment of Paraná coast. Were described 14 microhabitats occupied in different manners for 64 species of birds. Based on the results, could be suggested that this conspicuous class of vertebrates, together with the other elements that integrate the restinga, serve as a component to attract and stimulate the interest for the comprehension and necessity of conservation by the visitors of such ecosystem.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Microhabitat; Birds; Restinga; Sand ridges; Conservation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100013

Microhabitat preferences of six Drosera (Droseraceae) from Tibagi river basin, Paraná state, Brazil
Provedor de dados: 52
Autores: Saridakis,Dennis Panayotis; Torezan,José Marcelo Domingues; Andrade,Galdino.
We studied the microhabitats and the climatic requirements of six Drosera taxa, which occur in the subtropical grasslands of the Tibagi river basin. They are annual,or most communly, perennial herbs known as carnivorous plants, whose leaves are transformed into traps for the capture and digestion of small insects as an adaptation for supplying nutricional deficiencies. They usually occur in distrophic substrata. In order to contribute to conservation plans for the species of the genus, threatened by the conversion of the natural grasslands into pastures, we tried to define their preferential habitats and climatic requirements. We verified that the variables of microhabitat and altitude presented significant correlation at 5% probability level, while the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecology; Carnivorous plants; Drosera; Sundew; Brazil; Tibagi River Basin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400001

Is nuclear medicine really safe?
Provedor de dados: 52
In nuclear medicine, radiation absorbed dose estimates calculated by standard models at the whole body or organ are very low. At cellular level, however, the heterogeneity of radionuclide distributions of radiation dose patterns may be significant. We present here absorbed doses at cellular level and evaluate their possible impact on the usually assumed risk/benefit relationships in nuclear medicine studies. The absorbed dose values calculated are surprisingly high, and are difficult to interpret. In the present study, we show calculated doses at the cellular level and discuss possible biological consequences, for two radiopharmaceuticals labelled with technetium-99m: human serum albumin microspheres used for pulmonary scintigrapies and HMPAO used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Absorbed doses; Biological effects.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500016

Production of Propionic Acid by Propionibacterium acidipropionici from Agroindustrial Effluents
Provedor de dados: 52
Autores: Teles,Jéssica Cristine; Stolle,Elaine Macedo; Koloda,Stela Aparecida; Barana,Ana Claudia.
Abstract The purpose of this paper was to evaluate the production of propionic acid from the fermentation of agroindustrial effluents using a Propionibacterium acidipropionici culture. The composition of the substrates was determined by using an experimental design of mixtures, resulting in 10 trials. The substrates were fermented in batch borosilicate glass reactors at a temperature of 35°C, initial pH of 6.5, and 20 mL.L-1 of inoculum suspension. The highest yield of propionic acid production, 0.79 g of product per g of substrate, was obtained with a substrate composed only of corn steep liquor, which showed a productivity of 5.20 mg.L-1h-1 and production of 0.40mL.L-1. These results showed that the corn steep liquor positively influenced performance and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agroindustrial by-products; Fermentation; Corn steep liquor; Cheese whey; Wastewater.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100702

Diversity and seasonal changes in the ichthyofauna of rocky tidal pools from Praia Vermelha and São Roque, Santa Catarina
Provedor de dados: 52
Autores: Barreiros,João P.; Bertoncini,Áthila; Machado,Leonardo; Hostim-Silva,Maurício; Santos,Ricardo Serrão.
A survey of the tidal pool fish community was carried out between April 1998 and May 1999, at the beaches of Praia Vermelha (PV) and São Roque (SR), Santa Catarina, Brazil. The pool of PV was bigger and closer to the subtidal. Specimens were collected monthly at low tide, using quinaldine. In PV we collected 1287 fish belonging to 19 species and in SR 553 fish were caught corresponding to 11 species. In both pools, dominant species were Bathygobius soporator (Gobiidae), Abudefduf saxatilis (Pomacentridae), Parablennius pilicornis (Blenniidae), Gobiesox barbatulus (Gobiesocidae), Labrisomus nuchipinnis (Labrisomidae) and Epinephelus marginatus (Serranidae). Summer months had higher abundance and diversity values, and correspond to a recruitment season for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ichthyofauna; Rocky tidal pools; Diversity; Santa Catarina; Brazil.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200017

Enhanced Production Of Streptokinase By Chemical Mutagenesis Of Streptococcus agalactiae EBL-20
Provedor de dados: 52
Autores: Arshad,Arooj; Zia,Muhammad Anjum; Asghar,Muhammad; Joyia,Faiz Ahmad.
Abstract Streptokinase (SK) is an enzyme that is used for the treatment of cardiovascular diseases. The current study focused on the enhanced production of SK by inducing mutation in Streptococcus agalactiae EBL-20 and optimization of medium components and culture conditions for the maximum growth of mutant derived strain. S. agalactiae EBL-32 was selected as a potent mutant after exposure of S. agalactiae EBL-20 to EMS for 180 minutes. SK activity obtained from mutant derived strain was found to be 1.6 fold higher as compared to the activity achieved by wild strain. Nutritional requirements of the mutated strain were optimized by single factor analysis method suggesting glucose as the optimum carbon source; yeast extract and peptone as a suitable nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethyl methanesulfonate; Fermentation; Response surface methodology; Streptokinase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100200

Biotechnological approaches for plant viruses resistance: from general to the modern RNA silencing pathway
Provedor de dados: 52
Autores: Rodrigues,Silas Pessini; Lindsey,George G.; Fernandes,Patricia Machado Bueno.
Virus diseases are significant threats to modern agriculture and their control remains a challenge to the management of cultivation. The main virus resistance strategies are based on either natural resistance or engineered virus-resistant plants. Recent progress in understanding the molecular mechanisms underlying the roles of resistance genes has promoted the development of new anti-virus strategies. Engineered plants, in particular plants expressing RNA-silencing nucleotides, are becoming increasingly important and are likely to provide more effective strategies in future. A general discussion on the biotechnology of plant responses to virus infection is followed by recent advances in engineered plant resistance.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant defense; Engineered resistance; Phytopathogens.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400002

The use of the oregano (Origanum vulgare L.) essential oil and hydrosol in green olive fermentation
Provedor de dados: 52
Autores: Özcan,M. Musa; Arslan,Derya; Aydar,Ali Osman.
The effect of oregano the (Origanum vulgare L.) oil at the levels of 0.05, 0.1 and 0.3% and oregano hydrosol on the physicochemical, microbiological and sensory characteristics of the green olive (Edremit variety) fermentation was investigated. The initial pH of the oregano oil added samples were higher than the other treatments, which were above 5.8. The highest final acidity was observed in the hydrosol+brine combination (0.53%). The initial LAB population on the first day of the fermentation was high in the diluted hydrosol (8.89 log cfu ml-1) and control (8.47 log cfu ml-1) samples. But a significant difference was not observed between the LAB counts of the treatments on the 40th day of fermentation. The control and brine+oregano hydrosol samples had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green olive; Fermentation; Origanum vulgare L.; Essential oil; Hydrosol.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300022

Probiotics association in the suckling and nursery in piglets challenged with Salmonella typhimurium
Provedor de dados: 52
Autores: Afonso,Esther Ramalho; Parazzi,Larissa José; Marino,Carolina Tobias; Martins,Simone Maria Massami Kitamura; Araújo,Lucio Francelino; Araújo,Cristiane Soares da Silva; Vilela,Flávio Garcia; Moretti,Aníbal de Sant'Anna.
This work aimed to study the probiotics association in 144 piglets from birth to 62 days old. In lactation, the design was completely randomized with two treatments, CTL, 1 mL of distilled water and ProbA, 5g in 15 ml of distilled water, both orally, and in the nursery in randomized block design, with 2x3 factorial arrangement of treatments, ProbA ProbB: 30g/ton of ProbB in the ration; CTL ProbB: 30g/ton of ProbB in the ration; ProbA ProbA, CTL CTL; ProbA CHA (challenged); CTL CHA. At 35 days of age the animal of the nursery were inoculated with Salmonella typhimurium orally. There was no effect of the parameters evaluated during the maternity. In nursery, the feed conversion was favorable to the ProbA. In the evaluation of fecal score, the challenged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus subtilis; Bifidobacterium pseudolongum; Lactobacillus reuteri; Challenge. Piglets.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200010

The contribution of Andreas Mayer for the natural history of the State of Paraná, Brazil: V. Reptiles: relevant addenda and corrigenda
Provedor de dados: 52
Autores: Bérnils,Renato Silveira; Moura-Leite,Julio Cesar de.
A study about the herpetological legacy of the German naturalist Andreas Mayer (1907-1986) (Bérnils &amp;amp; Moura-Leite, 1990) raised 83 specimens from 20 reptile species captured in the Brazilian states of Paraná and Mato Grosso do Sul (years 40, 50, and 60) and currently housed at the Museu de História Natural Capão da Imbuia (Curitiba). Just in Mayer's birth centenary, 15 more snakes collected by him and not presented in the former publication were founded. This material is relevant because (1) came from areas currently under human-altered environments; (2) was collected in areas that were poorly sampled at that time; and (3) embraced some species hard to find in Paraná, including the first occurrence of Phimophis cf. guerini for the state. Label data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Squamata; Serpentes; Sauria; Andreas Mayer; Paraná; Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200023

Effects of croton urucurana extracts and crude resin on Anagasta kuehniella (Lepidoptera: Pyralidae)
Provedor de dados: 52
Autores: Silva,Luciana Barboza; Silva,Walciane; Macedo,Maria Ligia Rodrigues; Peres,Marize Terezinha Lopes Pereira.
Hundreds of plant species have been studied in order to find out the active ingredient responsible for their insecticidal activity against the pests of economic importance. To verify the insecticidal activity in the husk of stem of Croton urucurana Baillon 1864 (Euphorbiaceae) against Anagasta kuehniella Zeller 1879 (Lepidoptera: Pyralidae), the methanolic (EMeOH) extract, dichloromethane fraction (FDM), ethyl acetate fraction (FAE) and crude resin, incorporated into an artificial diet were evaluated. EMeOH (0.5, 1.0 and 2.0%) and crude resin (2.0%) interfered with neither the weight nor the survival of fourth instar larvae and other analyzed parameters. FDM (2.0%) fraction caused mortality of 65%, and the artificial diet containing 2.0, 1.0 and 0.5% FAE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbiaceae; Insecticide; 'sangra d'água'; Meal moth; Toxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300018

Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation
Provedor de dados: 52
Autores: Robl,Diogo; Mergel,Carla Montanari; Costa,Patricia dos Santos; Pradella,José Geraldo da Cruz; Padilla,Gabriel.
Abstract Tailor made enzymatic preparation must be design to hydrolyze efficiently plant biomass, once that each plant biomass possesses a distinct cell wall composition. Most of actinomycetes used for plant cell wall degradation are focused on the cellulases and xylanases production. However, a wide range of enzymes must be produced for an efficient degradation of lignocellulose materials. During the last decade several unusual environments were studied to obtain strains that produce glycohydrolases with innovator characteristics. In this context, the present work concerned the selection of endophytic actinomycetes as producers of hemicellulases and related enzymes with different enzymatic profiles, for use in the deconstruction of lignocellulosic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic actinomycetes; Hemicellulases; Accessory enzymes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100207

Performance of Differents Genotyps of Brazilian Orchid Cultivation in Alternatives Substrates
Provedor de dados: 52
Autores: Faria,Ricardo Tadeu de; Rego,Luciana do Valle; Bernardi,Anderson; Molinari,Hugo.
Two native Brazilian orchid especies, Oncidium baueri and Maxillaria picta, were grown in different substrate mixtures. The plants were cultivated in ceramic pots in a greenhouse with 50% of shadind light and watered three times a week. The following substrates were used: 1) de-fibered xaxim; 2)xaxim cubes; 3) vermiculite; 4) carbonized rice husk; 5) charcoal; 6) charcoal + carbonized rice husk; 7) crocks; 8) vermiculite and charcoal; 9) vermiculite + carbonized rice husks; 10) extruded polystyrene + charcoal; 11) pine bark + charcoal + extruded polystyrene; 12) vermiculite + carbonized rice husks + extruded polystyrene + charcoal; 13) pine bark. The substrate ratio was 1:1 in the mixture. NPK 10-10-10 leaf fertilizer was applied every thirty days and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orchidaceae; Maxillaria picta; Oncidium baueri; Growing media.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400003

Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4
Provedor de dados: 52
Autores: Krüger,Ernesto Renato; Penha,Tânia Regina; Hummelgen,Fernanda Cristina; Agottani,Jorge Bacila; Reva,Demétrio; Gonçalves,Robson; Thomaz-Soccol,Vanete.
ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-4; Serology; Diagnostic; Cattle; Epidemiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725

Liquid kit for preparation of 188rhenium-etidronate
Provedor de dados: 52
Autores: Marczewski,Barbara; Dias,Carla Roberta; Moraes,Vanessa; Osso Junior,João Alberto.
The aim of this study was the preparation of a liquid kit for radiolabeling of 188Re-HEDP (hydroxyethylidene diphosphonate). 188Re was obtained from alumina based 188W/188Re generators. This paper reports the efficacy of a cold kit stored for more than two weeks, determined by the dependence of the radiolabeling yields of 188Re-HEDP on the incubation time, reducing agent concentration, the effects of concentration of ligand, the pH of the reaction and the temperature. The cold kits showed a good stability when carrie-free rhenium-188 was added in the reaction mixture.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhenium-188 HEDP; Liquid Kit; Radiolabeling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700019

Physico-chemical evaluation of Rosmarinus officinalis L. essential oils
Provedor de dados: 52
Autores: Atti-Santos,Ana Cristina; Rossato,Marcelo; Pauletti,Gabriel Fernandes; Rota,Luciana Duarte; Rech,Juarez Ciro; Pansera,Marcia Regina; Agostini,Fabiana; Serafini,Luciana Atti; Moyna,Patrick.
Nineteen samples of Rosmarinus officinalis were extracted by steam distillation in a pilot plant and evaluated in terms of chemical compositions and physico-chemical characteristics. The volatile oil yields ranged from 0.37% (1999 harvest) to 0.49% (1998 harvest). Twenty components were identified in the oils. The major components were alpha-pinene (40.55 to 45.10%), 1,8-cineole (17.40 to 19.35%), camphene (4.73 to 6.06%) and verbenone (2.32 to 3.86%). The physico-chemical parameters averaged 0.8887 g/cm³ for specific gravity, 1.4689 for refractive index, and +11.82° for optical rotation, and there were no significant variations in either the chemical or physico-chemical data in the different years.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosmarinus officinalis rosemary essential oil composition alpha-pinene 1; 8-cineole physico-chemical parameters.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800020

The Effects of Different Media, Sucrose Concentrations and Natural Additives on Plantlet Growth of Phalaenopsis Hybrid 'Pink'
Provedor de dados: 52
Autores: Zahara,Meutia; Datta,Avishek; Boonkorkaew,Patchareeya; Mishra,Abha.
ABSTRACT The effects of various sucrose concentrations as carbon source and natural additives in different media on plantlet growth of Phalaenopsis hybrid 'Pink' were studied. Plantlets were cultured on two media (Murashige and Skoog [MS] and Vacin and Went [VW]) supplemented with 0, 10, 20, 30 and 40 g L-1 sucrose either with 0, 10 and 20% (v/v) coconut water (CW) or carrot juice (CJ) as natural additives. After four months of culture, the combination of sucrose and CW supplemented with both media affected plantlet growth where most of the plantlets showed slow growth and survival frequency (0-80%) with increasing concentrations of CW in all sucrose concentrations. However, plantlet growth on both media containing only 20 g L-1 sucrose without CW was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carrot juice; Coconut water; Natural additives; Phalaenopsis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100501

Influence of the 15-HETE on cytosolic [ Ca2+] i of the rabbit pulmonary artery smooth muscle cells
Provedor de dados: 52
Autores: Tang,Xiaobo; Zhang,Rong; Wang,Zhigang; Yu,Lei; Li,Shanshan; Zhu,Daling.
Extracellular Ca2+ influx was blocked by L-type Ca2+ channel blocker nifedipine to observe the effects of 15-hydroxyeicosatetraenoic acid on the constriction of rabbit pulmonary artery rings and on the changes of Ca2+ level in the rabbit pulmonary artery smooth muscle cells, and further to investigate the mechanism of the calcium mobilization induced by the 15-HETE under hypoxic conditions. The effect of extracellular Ca2+ on tension of the rabbit PA rings was also studied. Nifedipine (10 µ mol/L) had no effect on 1 µ mol/L 15-hydroxyeicosatetraenoic acid induced vasoconstriction under normoxic and hypoxic conditions. Intracellular Ca2+ increased markedly in the 15-HETE group (cells were exposed to 1 µ mol/L 15-HETE for 8 min during culture) compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 15-hydroxyeicosatetraenoic acid; Hypoxia; Pulmonary artery rings; Pulmonary artery smooth muscle cells; [ Ca2+]i.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200007

Transgenic Expression and Identification of Recombinant Human Proinsulin in Peanut
Provedor de dados: 52
Autores: Ling,Zheng; Qi-Qing,Jiao; Yu,Wang; Fei,Bian; Shu-Jie,Qu; Shu-Bo,Wan; Zhen-Ying,Peng; Yu-Ping,Bi.
The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery methods that rely on higher doses, is expected to result in a substantial escalation in the future demand for affordable insulin. Plant-based systems offer a safe and economical method for producing pharmaceutical proteins. We used peanut (Arachis hypogaea L.) as bio-reactors to express biosafe, stable proinsulin. We designed two proinsulin analogues (FAIA and LAIA) with substitutions in their amino acid sequences. The fast-acting insulin analogue (FAIA) contains a Gly inserted between Cys19 and Gly20, as well as a Pro28Asp substitution, in the B chain. The long-acting insulin analogue (LAIA) contains a Gly inserted between Cys19 and Gly20 and two Arg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proinsulin; Peanut; Diabetes; Expression.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100323

The correlation between the central carbon metabolic flux distribution and the number of shared enzyme regulators in Saccharomyces cerevisiae
Provedor de dados: 52
Autores: Zhou,Xiangfei; Liu,Lunxian; Shang,Chuanyu; Xu,Haifeng; Ding,Chao; Liu,Qian; Yi,Yin.
The central carbon metabolic system is the upstream energy source for microbial fermentation. In addition, it is a master switch for increasing the production of metabolites and an important part of the microbial metabolic network. Investigation into the relationship between genes, environmental factors, and metabolic networks is a main focus of systems biology, which significantly impacts research in biochemistry, metabolic engineering, and synthetic biology. To this end, the central carbon metabolic flux under a variety of growth conditions or using strains with various genetic modifications was previously measured in Saccharomyces cerevisiae using 13C tracer technology. However, the measured values were not integrated and investigated further. In this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Central carbon metabolic flux; Metabolic networks and pathways; Gene expression regulation; Gene environment interaction; Enzyme transcriptional regulator.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100415

Metabolism and Gaseous Exchanges in Two Coastal Lagoons from Rio de Janeiro with Distinct Limnological Characteristics
Provedor de dados: 52
Autores: Thomaz,Sidinei M.; Enrich-Prast,Alex; Gonçalves Jr.,José F.; Santos,Anderson M. dos; Esteves,Francisco A.
The global metabolism and exchange of gases with the atmosphere were measured during a diel cycle in two tropical coastal lagoons, using the curves of carbon dioxide and dissolved oxygen. Heterotrophic metabolism (net CO2 production and net O2 consumption) was observed in a black water lagoon (Comprida), and autotrophic metabolism (net O2 production and net CO2 consumption) in a clear water lagoon (Imboassica). These differences were attributed to the limnological characteristics of both ecosystems, especially to dissolved organic carbon and the attenuation coefficient of light, which are much higher in the first environment. During the diel cycle analyzed there was a net release of carbon dioxide into the atmosphere in the first lagoon and a net uptake by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal lagoon global metabolism heterotrophic activity microbial respiration; Diel variation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400015

Endophytic fungi occurring in Ipomoea carnea tissues and their antimicrobial potentials
Provedor de dados: 52
Autores: Tayung,Kumanand; Sarkar,Meenakshi; Baruah,Paran.
The aim of this work was to study the endophytic fungi associated with the tissues of Ipomoea carnea, a common invasive plant of India. A total of 69 isolates belonging to ten taxa comprising 1.45% Zygomycetes, 10.14% Coelomycetes, 62.32% Hypomycetes, 18.84% sterile mycelia and 7.25% unidentified species were obtained. Species of Curvularia, Aspergillus, Fusarium, Colletotrichum and sterile fungus were isolated as dominant endophytes. Colonization frequency of Curvularia (7.25%) was highest which was isolated from all the tissues. The samples collected during the monsoon harbored more endophytes and showed higher species richness than the samples obtained in summer season. Of the total isolates, 15 isolates (21.74%) displayed antimicrobial activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipomoea carnea; Endophytic fungi; Curvularia; Antimicrobial activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500003

Viability in the production of a drug extracted from Ananas comosus by a flat membrane system
Provedor de dados: 52
Autores: Lopes,Francisco Luiz Gumes; Sbruzzi,Dalva; Barros,Kleber Vanio Gomes; Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambourgi,Elias Basile.
The aim of this work was to study the production of e bromelain from the Ananas comosus L. Merril, by determining the process conditions using flat membranes. The production system modeling generated a hyperbolical curve and the optimization by response surfaces showed an influence of the transmembrane pressure higher than the pH influence. The cost of the production of bromelain from A. comosus was estimated 9 to 13 times lower than Sigma's retail sales price and 6.5 to 8.5 times lower than when this enzyme was obtained through a liquid-liquid extraction, which showed the economical feasibility of the process.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; Bromelain; Flat membrane; Production cost; Optimization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300018

Resistance to leaf rust in coffee carrying S H3 gene and others S H genes
Provedor de dados: 52
Autores: Sera,Gustavo Hiroshi; Sera,Tumoru; Ito,Dhalton Shiguer; Azevedo,José Alves de; Mata,João Siqueira da; Dói,Deisy Saori; Ribeiro Filho,Claudionor; Kanayama,Fabio Seidi.
The aim of this work was to evaluate the resistance to rust in coffee carrying S H3 gene and other S H genes. Twenty one CIFC’s coffee trees with several resistance genes S H were evaluated in field conditions. All the evaluated coffees carrying Sh3 gene presented resistance to the rust. It was possible that rust races with the virulence gene v3 in the Paraná State didn’t exist. The S H3 gene in combination with genes S H5, S H6, S H7, S H8, S H9 and S H? would be very important to obtain cultivars with more durable resistance to the rust.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemileia vastatrix; Cultivars; Breeding; Durable resistance; Coffee crop; Differential clones.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500002

A method to improve leaf succulence quantification
Provedor de dados: 52
Autores: Mantovani,André.
Leaf succulence has important physiological and ecological implications. Currently it is quantified by Delf's index (fresh weight/leaf area) and fresh weight/ dry weight ratio. Both indeces are reconsidered and a new index is proposed. Shade and sun leaves from terrestrial, hemiepiphytic and epiphytic aroids were studied. Delf's formula, which does not consider dry weight, overestimated leaf succulence. As fresh weight / dry weight ratio (fw / dw) does not consider leaf area, plants with the same fw / dw ratio were more than five times different in area. The last case was only possible with a decrease in surface / volume ratio and a increase of mesophyll thickness, components not measured by fw / dw ratio. The new index proposed here, which takes into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf succulence; Water relations; Leaf anatomy; Araceae; Ecophysiology.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100002

Comparison of the susceptibility of two hardwood species, Mimosa scabrella Benth and Eucalyptus viminalis labill, to steam explosion and enzymatic hydrolysis
Provedor de dados: 52
Autores: Ramos,L. P.; Carpes,S. T.; Silva,F. T.; Ganter,J. L. M.
Steam explosion of two hardwood species was carried out with and without addition of sulfuric acid (H2SO4 0.5%, p/v) as a pretreatment catalyst. In general, wood chips of Eucalyptus viminalis Labill were shown to be more amenable to pretreatment than chips derived from bolds of Mimosa scabrella Benth (bracatinga). This was apparent from all pretreatment parameters tested including the overall recovery yields of pretreated fractions, carbohydrates (pentoses and hexoses) recovered as water-solubles, yield of dehydration by-products and lignin susceptibility to acid hydrolysis. There was no evidence for complete deacetylation of both wood species during pretreatment and lignin appeared to undergo extensive acid hydrolysis at higher pretreatment severities....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus viminalis; Mimosa scabrella; Steam explosion; Pretreatment; Bioconversion.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200009

Molecular Analysis in the differentiation of Colletotrichum gloeosporioides isolates from the cashew and mango trees
Provedor de dados: 52
Autores: Serra,Ilka Márcia Ribeiro de Souza; Menezes,Maria; Coelho,Rildo Sartori Barbosa; Ferraz,Gabriela Moraes Guerra; Montarroyos,Angélica Virginia Valois; Martins,Luiza Suely Semem.
The aim of the present work was to analyze the molecular methods in the differentiation of Colletotrichum gloeosporioides isolates obtained from the cashew and mango trees. The different molecular taxonomic methods used proved to be efficient regarding intraspecific characterization. Similarly, molecular methods also proved to be efficient in differentiation of the C. gloeosporioides isolates in relation to host specificity. In the analysis of the ITS sequence of the ribosomal DNA, all the isolates amplified with the CgInt and ITS4 primers, confirming that they pertained to C. gloeosporioides. The results from this study suggested that methods based on the pathogenicity, isozyme analysis and RAPD were effective in differentiating C. gloeosporioides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anacardium occidentale; Anthracnose; ITS-rDNA; Mangifera indica.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600004

Identificação de fontes de resistência em cultivares de milho à spodoptera frugiperda (J. E. Smith, 1797) em casa-de-vegetação
Provedor de dados: 52
Autores: Silva,Deise Maria P. da; Oliveira,José V. de; Tabosa,José Nildo; Barros,Reginaldo; Santos,Elton O. dos; Azevedo,Simone S.
Dois experimentos foram estudados em Casa-de-Vegetação, com o objetivo de identificar fontes de resistência em 15 cultivares de milho à Spodoptera frugiperda. Utilizaram-se as cultivares CMS-35, CMS-36, Dina-70, Cargill-525, Pioneer-3210, Pioneer-6875, BR-201, BR-451, AG-106, AG-510, CMS-22, Contimax-322, Jatinã C3 Anão, Cargill-511 e BR-106. As plantas foram infestadas com 30 lagartas recém eclodidas por planta, quando estas apresentavam de 6 a 8 folhas. Após 10 e 14 dias da infestação, foram avaliados os danos através de uma escala visual de notas de 0 a 9. Não houve diferença estatística significativa entre as cultivares para os parâmetros estudados: dano, peso, comprimento e sobrevivência das lagartas. No entanto, as correlações lineares entre o peso x...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resistance; Zea mays; Foliar Damage; Fall armyworm; Larvae weight.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100014

Water quality assessment in piracicamirim creek upstream and downstream a sugar and ethanol industry through toxicity tests with cladocerans
Provedor de dados: 52
Autores: Botelho,Rafael Grossi; Machado Neto,Luís; Olinda,Ricardo Alves de; Dias,Carlos Tadeu; Tornisielo,Valdemar Luiz.
An environmental impact study was conducted to determine the Piracicamirim's creek water quality in order to assess the influence of effluents from a sugar industry in this water body. For this, toxicity tests were performed with a water sample upstream and downstream the industry using the microcrustaceans Daphnia magna, Ceriodaphnia dubia and Ceriodaphnia silvestrii as test organisms, as well as physical and chemical analysis of water. Results showed that physical and chemical parameters did not change during the sampling period, except for the dissolved oxygen. No toxicity was observed for D. magna and reproduction of C. dubia and C. silvestrii in both sampling points. Thus, the industry was not negatively impacting the quality of this water body.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daphnia magna; Ceriodaphnia dubia; Ceriodaphnia silvestrii; Water quality.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400020

Some Quality Parameters of Powdered Soups Prepared from Different Fish Species
Provedor de dados: 52
Autores: Öztürk,Fatma; Kalkan,Selin; Elmas,Ercan; Elmas,Sercan; Bariş,Pelin; Demir,Özlem.
Abstract In this study, we aim to determine the shelf life of dried and ready to use-powdered soup samples obtained from different types of fish species such as Rainbow trout (Oncorhynchus mykiss), sea bream (Sparusaurata L.) and sea bass (Dicentrarchuc labrax). For this purpose, the chemical (moisture, protein, fat, carbohydrate, ash, pH, Thiobarbituric acid (TBA), Total volatile basic nitrogen (TVB-N), microbiological (total mesophilic aerobic bacteria, total yeast and mold, total coliform, Staphylococcus spp. and Salmonella spp.) and sensory properties of fish soup samples were determined during 6-month storage period. The pH, TBA and TVB-N values of all samples increased during the storage period, but these values were within the consumption limits....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish soups; Thiobarbituric acid (TBA); Total volatile basic nitrogen (TVB-N); Storage; Quality.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100510

Higher than normal plasma Iinterleukin-6 concentrations in brazilian patients with mood disorders
Provedor de dados: 52
Autores: Miranda,Helen Cristina; Nunes,Sandra Odebrecht Vargas; Reiche,Edna Maria Vissoci; Oda,Julie Massayo Maeda; Watanabe,Maria Angelica Ehara.
The aim of this work was to study the plasma concentration of IL-6 by ELISA in the patients with mood disorders and normal healthy donors. The plasma concentration of IL-6 was higher in the patients than in the control healthy group. Results suggested that IL-6 could serve as an immunological marker in mood disorder pathogenesis as well.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-6; Cytokines; Mood disorder.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400010

Gravimetric determination of soil organic matter
Provedor de dados: 52
Autores: Miyazawa,M.; Pavan,M.A.; Oliveira,E.L. de; Ionashiro,M.; Silva,A.K.
Studies were carried out to evaluate a gravimetric method for the determination of soil organic matter by the mass loss at 300ºC. The gravimetric method was compared with Walkley-Black, using several brazilian soils with variable chemical and physical properties. Gravimetric method was positively correlated with Walkley-Black method with the following linear regression equation: y = 3.72x + 0.29, r = 0.94. The angular coefficient 3.72 for tropical soils was greater than those reported in the literature for temperate soils (from 1.68 to 2.13). The difference was due to greater oxidation degree of the organic matter. When compared with Walkley-Black method, gravimetric technique showed certain distinct advantages such as no environmental contamination with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic carbon; Thermogravimetry; Soil analysis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500005

Growth and photosynthetic capacity in two woody species of cerrado vegetation under different radiation availability
Provedor de dados: 52
Autores: Ronquim,Carlos Cesar; Prado,Carlos Henrique B. de A.; Paula,Nádia Figueiredo de.
Seedlings of two woody species of cerrado vegetation, Copaifera langsdorffii and Eriotheca gracilipes, were studied under three different radiation availability (100, 80 and 30% transmittance). Full solar radiation brought about more biomass, higher total leaf area, higher maximal photosynthesis per crown on area or on mass bases and higher photosynthetic capacity on area bases. Only the photosynthetic capacity values on leaf mass bases were higher in both species under low radiation availability (80 and 30% transmittance). The differences of accumulated biomass appeared more clearly after 4 months of sowing but the root/shoot dry biomass ratio and height were maintained constant for both species independently of the available radiation. Cultivated under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass allocation; Cerrado; Copaifera langsdorffii; Eriotheca gracilipes; Growth in shade; Photosynthesis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200016

Macroinvertebrates inhabiting the tank leaf terrestrial and epiphyte bromeliads at Reserva Adolpho Ducke, Manaus, Amazonas
Provedor de dados: 52
Autores: Torreias,Sharlene Roberta da Silva; Ferreira-Keppler,Ruth Leila.
The aim of this work was to investigate the diversity of macroinvertebrates and also verify if the abundance and diversity of Diptera were influenced by the abiotic factors. The samples were collected from the epiphytic and terrestrial bromeliads G. brasiliensis (1 and 3m) in wet and dry seasons at Reserva Adolpho Ducke analyzed total of 144 samples were analyzed from a total of 15,238 individuals collected. These conatined 14,097 insects and, among these, 8,258 were immature Diptera, represented by eight most abundant families: Chironomidae, Ceratopogonidae and Culicidae. The relationship of Diptera diversity was influenced by the seasons and stratifications (p= 0.01); the abundance was influenced by the volume of water (p= 0.02) and the relationship...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon; Bromeliaceae; Guzmania; Macroinvertebrates; Phytotelm.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600015

The Nano-Magnetic Dancing of Bacteria Hand-in-Hand with Oxygen
Provedor de dados: 52
Autores: Talib,Ayesha; Khan,Abid Ali; Ahmed,Haroon; Jilani,Ghulam.
ABSTRACT Magnetotactic bacteria are mostly microaerophilic found at the interface between oxic-anoxic zones. We report a magnetotactic bacterial strain isolated from an oil refinery sludge sample that grows aerobically in simple chemical growth medium, 9K. They open a new window of isolation of magnetic nanoparticles through an easy natural living system.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetotactic Bacteria; Bacillus; Aerobic; Magnetosomes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100429

Neurological and epidemiological aspects of a BoHV-5 meningoencephalitis outbreak
Provedor de dados: 52
Autores: Lunardi,Michele; Claus,Marlise Pompeo; Lisbôa,Júlio Augusto Naylor; Amude,Alexandre Mendes; Headley,Selwyn Arlington; Alfieri,Alice Fernandes; Alfieri,Amauri Alcindo.
Bovine herpesvirus 5 is a DNA virus that has been associated with meningoencephalitis in young cattle. While its clinical diagnosis is obscured by other major diseases that also produce similar neurological disease in cattle, the use of conventional virological techniques is hampered by the establishment of a lifelong latent infection in the host and the difficulty in differentiating BoHV-1 and BoHV-5. The aim of the current report is to describe the clinical and epidemiological aspects observed in a natural outbreak of BoHV-5 meningoencephalitis in a dairy cattle herd from Brazil. In the outbreak, the affected animals consisted of nine calves, which presented three possible forms of the neurological disease, subjectively classified as peracute, acute, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Central nervous system; Bovine herpesvirus 5; Multiplex-PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700011

The effect of red light on the germination of a Brazilian Pteridophyte
Provedor de dados: 52
Autores: Colli,Áurea M. T.; Perez,Sonia C. J. G. de A.
The effects of red light on the germination of spores of Cheilantes concolor Langsd &amp;amp; Fisch were investigated in this study. The spores were spread in the Mohr (1956) nutritional solution after Dyer’s modifications (1979). Three Petri dishes were used for each treatment, three slides per dish were made for each day of treatment, and one hundred spores per slide were counted . Germination of the spores in the dark was not observed. In relation to the photoperiods, the highest germination percentage and index values were obtained with the exposure of the spores to photoperiods of 8h, and the lowest values were obtained with their exposure to photoperiods of 2h. The phytochrome pigment acts in the germination of the spores through low fluency...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germination; Red light; Far-red light; Spores; Pteridophyte.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200006

Demography and structures population of Actinostemon concolor (Spreng.) Müll. Arg. (Euphorbiaceae) in flooded areas in Southern Brazil
Provedor de dados: 52
Autores: Bianchini,Edmilson; Araújo,Carla Gomes de; Green,Márcia; Pimenta,José Antonio.
The aim of this work was to analyze the size and spatial structures and demography of Actinostemon concolor (Spreng.) Müll. Arg. (Euphorbiaceae) in the flooded areas of Mata dos Godoy State Park. Fifty plots of 100 m² were established and three surveys were conducted. All the individuals were measured, mapped and divided into categories as seedlings, juveniles and adults. The coefficient of skewness, L-function of Ripley, and the matrix population models were used in the study. The population showed positive coefficient of skewness and the individuals were distributed in groups. The population growth rates (λ) were equal or lower than 1.0 and the adult survival and recruitment of juvenile to adult were the vital rates that most affected the values of λ....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elasticity; Matrix models; Spatial distribution; Structure size; Tropical tree.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100009

Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose
Provedor de dados: 52
Autores: Pereira Júnior,José Antônio de Sousa; Correia,Marcos José; Oliveira,Neiva Tinti de.
Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lentinula edodes; Cellulase; Liquid medium cultivation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300004

Formulation and characterization of gastroretentive discs containing famotidine
Provedor de dados: 52
Autores: Raval,Jignyasha Amitkumar; Patel,Madhabhai M.
In this work a different type of formulation, as disc, containing a selected mucoadhesive polymer, fillers, and binders were investigated for their potential as a mucoadhesive gastroretentive delivery system to deliver famotidine in the stomach. Various types of hydrophilic diluents were evaluated for their swelling and mucoadhesive property and one (polyvinylpyrrolidone, PVP) was selected to combine with the selected mucoadhesive polymer (polyethylene oxide, PEO). Discs with different ratios of PEO and PVP were prepared and evaluated for swelling, dissolution, and mucoadhesion. The swelling property of the discs increased as the concentration of PEO was increased and also did the mucoadhesion. These discs retained their integrity and adherence onto...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucoadhesion; Discs; Gastroretentive; Polyethylene oxide; Polyvinylpyrrolidone.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200010

Influence of starch and casein administered postruminally on small intestinal sodium-glucose cotransport activity and expression
Provedor de dados: 52
Autores: Guimaraes,Kátia Cylene; Hazelton,Sarah Rodrigues; Matthews,James Clyde; Swanson,Kendall Carl; Harmon,David Lee; Branco,Antonio Ferriani.
Twenty-four Holstein steer calves (88 ± 3kg) with abomasal catheters were randomly assigned within blocks to one of four treatments. The treatments consisted of four abomasal infusions: water (control), 4 g/kg BW.d-1 of partially hydrolyzed starch (SH), 0.6 g/kg BW.d-1 of casein, and a mix of SH + casein. The small intestine was collected and five equidistan, 1m sites were identified (duodenum, jejunum 1, jejunum 2 jejunum 3 and ileum). Maltase specific activity in mucosal homogenate and brush border membrane vesicles, SGLT1 protein abundance, and sodium-dependent glucose uptake in brush border membrane vesicles did not differ between the calves receiving different abomasal infusion treatments. However, maltase specific activity in homogenates and brush...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Glucose; Maltase; Sodium-dependent glucose co-transporter; Starch.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700007

Influence of water level fluctuation on the mortality and aboveground biomass of the aquatic macrophyte Eleocharis interstincta (VAHL) roemer et schults
Provedor de dados: 52
Autores: Santos,Anderson Medeiros dos; Esteves,Francisco de Assis.
The goal of this study was to study the biometric alterations of Eleocharis interstincta in response to water level fluctuations in Cabiúnas Lagoon, located on the northern coast of the state of Rio de Janeiro, in the municipality of Macaé. Three quadrats of 0.0625 m² were harvested every two weeks from June/1997 to June/1998; samples were separated into stems, dead stems (detritus) and rhizome; lenghted, dried and weighted. The water level fluctuated seasonally in the macrophyte stand with two periods of drawdown. The first period occurred naturally at the end of winter and beginning of spring, when rainfall in the area was normally lowest. The second period of drawdown was the result of an artificial breaching of the sandbar that isolate the lagoon from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic macrophytes; Biomass; Coastal lagoon; Eleocharis interstincta; Water level fluctuation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200016

In vitro inhibition of adhesion of Escherichia coli strains by Xylitol
Provedor de dados: 52
Autores: Silva,Annelisa Farah da; Suzuki,Érika Yoko; Ferreira,Aline Siqueira; Oliveira,Murilo Gomes; Silva,Sílvio Silvério da; Raposo,Nádia Rezende Barbosa.
The present study aimed to evaluate xylitol's antimicrobial and anti-adherence activities on Escherichia coli (ATCC 8739) and on another clinical strain enteropathogenic E. coli (EPEC). In vitro minimum inhibitory concentration (MIC) test and adhesion assays were performed using 0.5, 2.5 and 5.0% xylitol. It was found that xylitol did not have antimicrobial properties on these strains. The scanning electron microscopy (SEM) demonstrated that the slides treated with xylitol had a significant reduction in the number of bacilli and the inhibition of microbial adhesion was probably the xylitol's mechanism of action. Xylitol could be a possible alternative on the control of E. coli infections.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial adhesion; Escherichia coli; Scanning electron microscopy; Xylitol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200003

Effects of aerial exposure on antioxidant defenses in the brown mussel Perna perna
Provedor de dados: 52
Autores: Almeida,Eduardo Alves de; Bainy,Afonso Celso Dias.
Investigations were carried out to evaluate the antioxidant defenses in digestive gland of mussels Perna perna held in air for 4 hours, exposed to air for 4 hours followed by submersion in water for 30 minutes, and constantly submerged for 4.5 hours. No differences were observed in CAT and GPx activities and in the levels of total GSH. Mussels exposed to air had significantly higher SOD activity, possibly related to a preparative mechanism of defense against oxidative stress during reoxygenation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Hypoxia; Mussel; Perna perna; Aerial exposure.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300007

Antidiabetic activities of ethanol extract of dry matters of culture broth of Coriolus versiolor in submerged culture
Provedor de dados: 52
Autores: Zhang,Zuofa; Lv,Guoying; Pan,Huijuan; Santos,Leandro Freire dos; Fan,Menghai; Soccol,Carlos Ricardo; Thomaz-Soccol,Vanete; Lacerda,Luiz Gustavo; Carvalho Filho,Marco Aurélio da Silva; Fan,Leifa.
The study aimed to investigate the antihyperglycemic and antilipidperoxidative effects of ethanol extract of the dry matter from culture broth of Coriolus versicolor (ECBC) in streptozotocin-induced diabetes mice. Blood glucose level, insulin level, total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol(LDL-C), high density lipoprotein -cholesterol (HDL-C) in serum and reduced glutathione level (GSH), lipid peroxidation, glycogen, antioxidant enzymes in liver were evaluated. Moreover, histopathological observation was conducted. Streptozotocin treatment (150 mg/kg body weight) induced the decrease of GSH level, antioxidant enzymes activities, glycogen content in liver, HDL-C content and insulin level in serum, accompanied by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coriolus versiolor; Antihyperglycemic; Antilipidperoxidative; Diabetes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400008

Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
Provedor de dados: 52
Autores: Almeida,Andréa Farias de; Macedo,Gorete Ribeiro de; Chan,Leslie Chee Loong; Pedrini,Márcia Regina da Silva.
In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; In vitro production; Kinetic analysis; SfMNPV.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200006

Early and Accurate Model of Malignant Lung Nodule Detection System with Less False Positives
Provedor de dados: 52
Autores: Krishnamurthy,Senthilkumar; Narasimhan,Ganesh; Rengasamy,Umamaheswari.
ABSTRACT The objective of this work is to identify the malignant lung nodules accurately and early with less false positives. ‘Nodule’ is the 3mm to 30mm diameter size tissue clusters present inside the lung parenchyma region. Segmenting such a small nodules from consecutive CT scan slices are a challenging task. In our work Auto-seed clustering based segmentation technique is used to segment all the possible nodule candidates. Efficient shape and texture features (2D and 3D) were computed to eliminate the false nodule candidates. The change in centroid position of nodule candidates from consecutive slices was used as a measure to remove the vessels. The two-stage classifier is used in this work to classify the malignant and benign nodules. First stage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung cancer; 3-D Image Segmentation; 3-D image features; Volume growth; Lung nodule classifier.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100308

Occurrence of plastic particles in procellariiforms, south of São Paulo state (Brazil)
Provedor de dados: 52
Autores: Barbieri,Edison.
Seabirds ingest plastic particles floating on the surface of the world's oceans. The birds can ingest plastic particles that they mistakenly identify as prey items. Alternatively, plastics can be taken up in the stomach contents of prey species. Plastic is often passed from parents to chicks in regurgitated food. In this study, individual petrels and albatrosses brought in by the tide onto Ilha Comprida beach between January 2000 and December 2002 were collected. Ilha Comprida, a barrier island in southern São Paulo, Brazil, was traversed by car along a transect of 70 kilometers. A total of 110 birds of 10 species were collected and 64.54% had plastic particles in their stomach. Frequencies of occurrence were Macronectes giganteus (64.28%), Thalassarche...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Contamination; Ilha Comprida; Plastic; Seabirds; Procellariiformes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200011

Identificação de fontes de resistência de milho à Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) em campo
Provedor de dados: 52
Autores: Silva,Deise Maria P. da; Oliveira,José V. de; Tabosa,José Nildo; Barros,Reginaldo; Santos,Elton O. dos; Azevedo,Simone S.
Com o objetivo de identificar fontes de resistência à Spodoptera frugiperda (J. E. Smith, 1797), foram avaliadas as cultivares de milho CMS-22, CMS-35, AG-106, Dina-70, AG-510 e CMS-36 em campo, utilizando-se o delineamento experimental de blocos ao acaso com quatro repetições. Utilizaram-se as variáveis: danos foliares, obtidos através da escala visual de notas, sobrevivência, peso e comprimento das lagartas, 10 dias após a infestação artificial das plantas com lagartas de 1º ínstar. As cultivares diferiram significativamente (P=0,05) apenas com relação aos danos, destacando-se AG-510 como a mais resistente, com dano médio de 5,41 em comparação com CMS-35 e AG-106, as mais suscetíveis, com danos médios de 8,38 e 7,85 respectivamente. Foram determinadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Fall armyworm; Plant resistance; Foliar damage; Larvae survival.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300015

Comparison between canopy trees and arboreal lower strata of urban semideciduous seasonal forest in Araguari - MG
Provedor de dados: 52
Autores: Souza,João Paulo de; Araújo,Glein Monteiro; Schiavini,Ivan; Duarte,Polyana Custódio.
The present study intended to determine the phytosociological characteristics of the tree elements on the lower strata and the canopy of a semideciduous seasonal forest. The trees (&gt; 15 cm circumference at breast height) were sampled in 40 plots of 10m x 20m. The individuals in the lower strata (&gt; 1m up to 15 cm of circumference in the base of the stem) were sampled in plots of 10m x 10m. Licania apetala and Micrandra elata obtained the first and second places in the two strata. Among the species with the highest value of importance on the canopy, Alchornea glandulosa, Pimenta pseudocaryophyllus, Copaifera langsdorffii, Heisteria ovata and Didymopanax morototoni presented a ratio of less than one individual in the lower strata when compared to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semideciduous forest; Lower strata; Canopy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600012

Catechol biodegradation kinetics using Candida parapsilopsis
Provedor de dados: 52
Autores: Rigo,Maurício; Alegre,Ranulfo Monte; Bezerra,José Raniere Mazile Vidal; Coelho,Narjara; Bastos,Reinaldo Gaspar.
This study evaluated the biodegradation of catechol by a yeast strain of Candida parapsilopsis in standard medium in Erlenmeyer flasks. Results shown that the highest concentration of catechol caused the longer lag period, demonstrating that acclimatized cultures could completely degrade an initial catechol concentration of 910 mg/L within 48 h. Haldane's model validated the experimental data adequately for growth kinetics over the studied catechol concentration ranges of 36 to 910 mg/L. The constants obtained for this model were µmax = 0.246 h-1, Ks = 16.95 mg/L and Ki = 604.85 mg/L.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida parapsilopsis; Biodegradation; Catechol; Growth kinetics; Equation of Haldane; Substrate inhibition.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200029

Evaluation of a functional soy product with addition of soy fiber and fermented with probiotic kefir culture
Provedor de dados: 52
Autores: Baú,Tahis Regina; Garcia,Sandra; Ida,Elza Iouko.
The objective of this study was to evaluate the chemical, sensory properties and stability of a functional soy product with soy fiber and fermented with probiotic kefir culture. The product was characterized by the chemical composition, color and sensory analysis. The stability of the product was evaluated by pH, acidity, viscosity, firmness, syneresis measurements and cells counts. The functional soy product presented better chemical composition and difference in color compared to the fermented product without fiber. Sensory analysis showed that the functional soy product had good acceptance and had better firmness and reduced syneresis compared to fermented product without fiber. The lactic acid bacteria counts decreased slightly during 28 days at 4°C of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soy fiber; Kefir culture; Functional fermented soy product; Storage; Probiotic product.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300014

Savitzky-Golay Filter for Denoising Lung Sound
Provedor de dados: 52
Autores: Haider,Nishi Shahnaj; Periyasamy,R; Joshi,Deepak; Singh,B K.
ABSTRACT For computerized analysis of respiratory sounds to be effective, the acquired signal must be free from all the interfering elements. Different forms of noise which can degrade the quality of lung sounds are recording artifacts, power line/Radio Frequency (RF) interferences, ambient acoustic interferences, heart sound interference etc. Such interferences adversely affect the diagnostic interpretations. Powerful denoising techniques are necessary to resolve this issue. A denoising scheme for lung sounds, based on Savitzky-Golay (S-G) filter is proposed in this paper. The order and frame length of the SG filter is determined objectively using the Signal to Noise Ratio (SNR) and computational time as objective function. Maximum SNR is observed when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Denoising; Frame length; Lung sound; Polynomial order; Savitzky-Golay filter.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100326

Role of Carnosine and Melatonin in Ameliorating Cardiotoxicity of Titanium Dioxide Nanoparticles in the Rats
Provedor de dados: 52
Autores: Al-Rasheed,Nouf; Faddah,Laila; Ibrahim,Hanan; Mohamed,Azza M; Al-Rasheed,Nawal; Abdelbaky,Nayira.
The aim of this work was to study the possible cardiotoxicity of two different doses of 50 nm nano titanium dioxide (n-TiO2) and the possible modulating effects of the use of two natural antioxidants carnosine and melatonin. The results showed that TiO2- NPs produced deleterious effects on rat cardiac tissue as confirmed by the increased levels of serum myoglobin, troponin-T and CK-MB. Increased levels of serum Inflammatory markers represented by the tumor necrosis factor alpha (TNF-α) and Interleukin-6 (IL-6) was also noticed. Caspase3 and IGg were elevated compared to the control group in a dose dependant manner. treatment of the rats with Carnosine or melatonin. along with TiO2- NPs administration significantly improved most of the elevated biochemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Titanium dioxide nanoparticles; Cardiotoxicity; Carnosine; Melatonin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400577

Homology modelling and insilico analysis of neuraminidase protein in H1N1 Influenza A virus
Provedor de dados: 52
Autores: Manohar,Abhilash.
In this work, modelling of Neuraminidase protein of Influenza A virus (A/Himeji/1/2009(H1N1)) neuraminidase (NA) protein was done using Modeller 9V2. Modelled structure was submitted to protein model database and could be downloaded using accession number PM0075830. The modelled protein structure was subjected to In silco analysis using various bioinformatics tools. Two anti-influenza drugs currently being used to treat infected patients are oseltamivir (Tamiflu) and zanamivir (Relenza), both of which target the neuraminidase enzyme of the virus. Reports of the emergence of drug resistance make the development of new anti-influenza molecules a priority. Hence the modelled structure of H1NI Neuraminidase could be very useful for in silico analysis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H1N1; Neuraminidase; Modelling; In silico analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100003

An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species
Provedor de dados: 52
Autores: Alzate-Marin,Ana Lilia; Guidugli,Marcela Corbo; Soriani,Hilda Hildebrand; Martinez,Carlos Alberto; Mestriner,Moacyr Antônio.
An efficient and rapid DNA minipreparation modified method for frozen samples was developed for five tropical tree species: Copaifera langsdorffii, Hymenaea courbaril, Eugenia uniflora, Tabebuia roseo alba and Cariniana estrellensis. This procedure that dispenses the use of liquid nitrogen, phenol and the addition of proteinase K, is an adaptation of the CTAB-based DNA extraction method. The modifications included the use of PVP to eliminate the polyphenols, only one chloroform-isoamyl alcohol step and the addition of RNase immediately after extraction with chloroform. The yields of the DNA samples ranged from 25.7 to 42.1 µg from 100 mg leaf tissue. The DNA samples extracted by this method were successfully used for PCR (SSR and RAPD) analyses in these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copaifera langsdorffii; Hymenaea courbaril; Eugenia uniflora; Tabebuia roseo alba; Cariniana estrellensis; DNA extraction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500020

Selection of cellulolytic fungi isolated from diverse substrates
Provedor de dados: 52
Autores: Damaso,Mônica Caramez Triches; Terzi,Selma da Costa; Farias,Antonio Xavier; Oliveira,Ana Carolina Pereira de; Fraga,Marcelo Elias; Couri,Sonia.
The aim of the present work was to select filamentous fungi isolated from diverse substrates to obtain the strains with potential to produce the hydrolytic enzymes. From a total of 215 strains, seven strains from the soils, six from the plants and one from sugarcane bagasse were selected and identified as belonging to the Trichoderma, Penicillium and Aspergillus genera. The best hydrolytic activities obtained by semi-solid fermentation using these strains were approximately: 35; 1; 160; 170 and 120 U/gdm (CMCase, FPase, β-glucosidase, xylanase and polygalacturonase, respectively), demonstrating their potential to synthesize the enzymes compared with the results reported in the literature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulases; Semi-solid fermentation; Filamentous fungi; Hydrolytic enzymes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400005

Nitrogen balance of starting barrow pigs fed on increasing lysine levels
Provedor de dados: 52
Autores: Moreira,Ivan; Fraga,Alessandro Luis; Paiano,Diovani; Oliveira,Gisele Cristina de; Scapinello,Cláudio; Martins,Elias Nunes.
The effects of increasing lysine levels on nitrogen balance of pigs fed on low protein diets were evaluated. Four treatments (diets) containing lysine levels (0.8, 1.0 1.2 and 1.4%) were applied to 12 starting (20.0 ± 1.8 kg) barrow pigs. Methionine, threonine and tryptophan were kept constant to the lysine ratio in all diets. Feces and urine were collected during a 5-day period. Nitrogen output in urine (NOU), total nitrogen output (TNO), nitrogen retention (NR), net protein utilization (NPU), biological value or feed protein (BVFP), urine urea nitrogen (UUN), and plasma urea nitrogen (PUN) were determined. PUN showed high negative correlations with BVFP (-0.84), NPU (-0.76), and NR (-0.78) and a positive correlation (0.79) to NOU. Lowest nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental pollution; Crystalline amino acids; Protein metabolism; Slurry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100012

Distribution of copper in soil and rice system of Hainan Island, China
Provedor de dados: 52
Autores: Dengfeng,WANG; Haijie,HUANG; Huande,FENG; Hua,WANG; Miaojie,HAN; Zhiping,QI.
ABSTRACT This research aimed to discover the distribution and the primary influence factors of Cu in the soil-rice systems of tropical farmland. Soil samples of farmland and rice plant (stalk and polished rice) from the western region of Hainan Island were collected and studied. The results showed that the average Cu content in the topsoil of the study area was 15.75 mg kg-1; the highest Cu content (45.92 mg kg-1) was found in the rice fields of the northern area, where pyroclastic parent material is distributed. Thus, there is a potential for Cu contamination of the rice grown in this region. The average contents of Cu in the rice stalks and polished rice were 16.9 and 5.68 mg kg-1, respectively, indicating that the stalks had a larger capacity for Cu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice; Copper; Arable land; Hainan.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200703

Production of pectinases by A. niger: influence of fermentation conditions
Provedor de dados: 52
Autores: Martos,María A.; Martinez Vazquez,Francisco; Benassi,Fernando O.; Hours,Roque A.
Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinases; Polygalacturonase; Pectinesterase; Aspergillus niger; Response surface methodology; Citrus pectin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300007

Walker-256 Tumor: Experimental Model, Implantation Sites and Number of Cells for Ascitic and Solid Tumor Development
Provedor de dados: 52
Autores: Amaral,Luane Aparecida do; Souza,Gabriel Henrique Oliveira de; Santos,Mirelly Romeiro; Said,Yasmin Lany Ventura; Souza,Bruna Brandão de; Oliveira,Rodrigo Juliano; Santos,Elisvania Freitas dos.
Abstract The Walker-256 tumor is an important experimental model that allow the development of therapies as the biological behavior of this tumor is similar that occur in humans. In front of the above considerations, the aim of this study was to describe the experimental model of Walker-256 tumor, identify the implantations sites as well as define a usual quantity of tumoral cells to induce the ascitic and solid tumor, according to the specialized literature. Were selected 45 articles using the keyword “Walker-256 tumor”, free available. Were possible to observe that 58% (n=26) of the studies inoculate the tumor cells in the animals flank 33% (n = 15) in the tibia bone, 7% (n = 3) in the femur and 2% (n = 1) in the paw. The major quantitates of cells used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Walker-256 tumor; Cancer; Experimental model; Rats.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100900

Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis Cotiledonary culture
Provedor de dados: 52
Autores: Quisen,Regina; Oliveira,Yohana de; Pileggi,Marcos; Cuquel,Francine; Quoirin,Marguerite.
The objectives of the present work were to establish the minimal lethal dose of the selective agent to determine the type and concentration of appropriate antibiotics for the elimination of Agrobacterium tumefaciens inoculated explants, without interfering with the regenerative potential of the E. camaldulensis cotyledonary explants. Non-transformed explants were cultivated in medium supplemented with kanamycin. The results showed that the antibiotic was suitable for the selection of transformed cells in the concentration of 9 mg L-1 as it inhibited the growth of non-transformed cells. Cotyledons infected with A. tumefaciens were cultivated in MS N/2 medium supplemented with BAP, ANA, Km and cefotaxime or AugmentinÒ . The highest average of regenerated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphogenesis; Kanamycin; Cefotaxime; Augmentin; Agrobacterium tumefaciens; Eucalypt.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600020

Ultrastructural analysis of kidney, liver and duodenum isolated from treated rats with Ginkgo Biloba extract and effects of this medicinal plant on the biodistribution of the padiopharmaceutical sodium pertechnetate
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Nascimento,Ana Lúcia; Arnobio,Adriano; Olej,Beni; Timóteo,Margareth de Oliveira; Caldas,Luiz Querino de Araújo; Bernardo Filho,Mário.
Ginkgo biloba extract (EGb) has been used to treat memory and concentration deficits, acts as platelet activating factor antagonism and prevents against damages caused by free radicals. EGb is a standardized extract that contains 24% flavonoids and 6% terpenoids. The aim of this work was to evaluate the possible influence of an EGb on the ultrastructure of some organs isolated from rats and on the biodistribution of sodium pertechnetate (99mTcO4Na). The animals were treated with EGb and after six days, received 99mTcO4Na. The organs were isolated and fixed for ultrastructural analysis. The results showed that EGb has modified the ultrastructure of kidney, liver and duodenum and altered the biodistribution of 99mTcO4Na (P&lt;0.05). It is speculated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgo biloba; Technetium-99m; Biodistribution; Morphology; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700030

Redistribution of phosphorus in soil through cover crop roots
Provedor de dados: 52
Autores: Franchini,Júlio C.; Pavan,Marcos A.; Miyazawa,Mário.
The objective of this study was to evaluate if cover crops can absorb P from the upper layers and transport it in their roots to subsoil layers. Samples of an Oxisol were placed in PVC columns. Super phosphate fertilizer was applied to the 0-10 cm soil surface layers. The cover crops tested were: Avena strigosa, Avena sativa, Secale cereale, Pisum sativum subsp arvense, Pisum sativum, Vicia villosa, Vicia sativa, Lupinus angustifoliu, Lupinus albus, and Triticum aestivum. After a growth period of 80 days the cover crop shoots were cut off and the soil was divided into 10cm layers and the roots of each layer were washed out. The roots and shoots were analyzed separated for total P contribution to the soil. Considerable amount of P was present in the roots...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphorus nutrition; Soil phosphorus; Green manure; No-tillage.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300007

Nutritional requirements of Zymomonas mobilis CCT 4494 for levan production
Provedor de dados: 52
Autores: Ernandes,Fernanda Maria Pagane Guereschi; Garcia-Cruz,Crispin Humberto.
The aim of this work was to study the nutritional requirements of Zymomonas mobilis CCT 4494 for levan production in three chemically defined media. During the optimization of the fermentative process for the production of the exopolysaccharide, different concentrations of glucose, fructose and sucrose as carbon source and yeast extract as vitamin source were tested. Variations of incubation temperature and initial pH of the medium were observed. The results showed that medium containing 20.0% sucrose and 0.5% yeast extract, with initial pH of 7.0, incubated at 30°C gave a 43.0% yield of the biopolymer.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymomonas mobilis; Levan; Exopolysaccharide; Production.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300021

Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater
Provedor de dados: 52
Autores: Elahi,Amina; Rehman,Abdul.
ABSTRACT Trichosporon asahii and Rhodotorula mucilaginosa isolated from wastewater effluents were identified as chromium-resistant yeasts. Cr(VI) concentrations at 8 mM and 6 mM were inhibitory for R. mucilaginosa and T. asahii. Remarkably elevated GSH (69.88 ± 10.01) and GSSG (11.24 ± 0.96) was observed under metal stress in T. asahii as compared to R. mucilaginosa GSH (18.95 ± 3.19) and GSSG (3.7 ± 2.74) mM g-1 8 level. Statistical analysis revealed significantly higher GSH/GSSG ratio in both strains. NPSH (29.84 ± 0.54) level in T. asahii was much higher than in R. mucilaginosa (6.05 ± 0.24). Chromate reductase (ChR) was assayed and its activity was optimum at 50°C (pH 6) in T. asahii while R. mucilaginosa showed higher activity at 30°C (pH 7). Activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichosporon asahii; Rhodotorula mucilaginosa; Wastewater; Resistance; Bioaccumulation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100452

Technetium-99m-labeled stealth pH-sensitive liposomes: a new strategy to identify infection in experimental model
Provedor de dados: 52
Autores: Carmo,Vildete Aparecida Sousa; Oliveira,Mônica Cristina de; Mota,Luciene das Graças; Freire,Luís Paulo; Ferreira,Raphael Ligório Benedito; Cardoso,Valbert Nascimento.
The diagnosis of inflammatory and infectious processes is an important goal in medicine. The use of radiopharmaceuticals for identification of inflammation and infection foci has received considerable attention. The aim of this work was to evaluate the uptake and the imaging potential of stealth pH-sensitive liposomes radiolabelled with 99mTechnetium (99mTc) to identify infection sites in mice. The liposomes containing glutathione were labeled with 99mTc-Hexamethylpropyleneamine oxime (HMPAO) complex. The 99mTc-labeled stealth pH-sensitive liposomes (99mTc-SpHL) were injected in mice bearing infection in the right thigh muscle induced by Staphylococcus aureus. Biodistribution studies and scintigraphic imaging were performed at different times after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 99mTechnetium; PH-sensitive liposomes; Infection imaging; Scintigraphy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600025

Phenolic Profile, Essential Oil Composition and Bioactivity of Lasia spinosa (L.) Thwaites
Provedor de dados: 52
Autores: Rahman,Atiqur; Siddiqui,Shah Alam; Oke-Altuntas,Feyza; OKAY,Sezer; GÜL,Fatih; Demirtas,Ibrahim.
Abstract Lasia spinosa (L.) Thwaites is a widely used ethnomedicinal plant in Bangladesh. In this study, we investigated phenolic contents, volatile compounds and fatty acids, and essential oil components of extracts prepared from aerial parts of the plant. The main volatile compounds were methyl ester of oleic acid, palmitic acid and stearic acid as determined by GC/MS. Phenolic contents of the extracts were determined qualitatively and quantitatively by HPLC/TOF-MS. Six phenolic compounds (syringic acid, morin, gentistic acid, 4-hydroxybenzoic acid, cinnamic acid, and apigenin) were found in the extracts. GC/MS analysis of steam distilled essential oil showed camphor, α-pinene and δ-3-carene as the main constituents. In DPPH radical scavenging assay, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GC/MS; HPLC/TOF-MS; DPPH radical scavenging activity; Metal chelating; MIC.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100405

In Vitro antifungal potency of plant extracts against five phytopathogens
Provedor de dados: 52
Autores: Tapwal,Ashwani; Nisha,; Garg,Shipra; Gautam,Nandini; Kumar,Rajesh.
The antifungal activity of aqueous extract of Cannabis sativa, Parthenium hysterophorus, Urtica dioeca, Polystichum squarrosum and Adiantum venustum was investigated against Alternaria solani, Alternaria zinniae, Curvularia lunata, Rhizoctonia solani and Fusarium oxysporum at different concentrations (5, 10, 15 and 20%). At 20%, maximum antifungal potential was observed with the extracts of C. sativa, which recorded excellent inhibitory activity against C. lunata (100%), A. zinniae (59.68%), followed by leaf extract of P. hysterophorus (50%) against A. solani. The application of botanical extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botanical; Phytoextract; Antifungal; Phytopathogen; IPM.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600003

Dietary models for inducing hypercholesterolemia in rats
Provedor de dados: 52
Autores: Matos,Sheyla Leite; Paula,Heberth de; Pedrosa,Maria Lúcia; Santos,Rinaldo Cardoso dos; Oliveira,Eduardo Luiz de; Chianca Júnior,Deoclécio Alves; Silva,Marcelo Eustáquio.
The present work aimed at finding a dietetical model capable of promoting the highest hypercholesterolemia without affecting the development of the rats. Sixty female Fisher rats were divided into five groups. The first one was fed a control diet; the remaining four were fed hypercholesterolemic diets with cholesterol and different contents of soybean oil, starch, casein, micronutrients and fiber and, consequently, different caloric values. After eight weeks animals were evaluated in relation to growth, fecal excretion, liver weight and fat, cholesterol and its fractions, serum biochemical parameters and sistolic pressure and compared with controls. The best result was obtained with the diet containing 25 % soybean oil, 1.0 % cholesterol, 13 % fiber and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypercholesterolemia; Animal model; Caloric value; Cholesterol.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200006

A Biosensor Using Poly(4-Aminophenol)/acetylcholinesterase modified graphite electrode for the detection of dichlorvos
Provedor de dados: 52
Autores: Melo,Edmar Isaías; Franco,Diego Leoni; Afonso,André Santiago; Rezende,Hélen Cristine; Brito-Madurro,Ana Graci; Madurro,João Marcos; Coelho,Nívia Maria Melo.
The properties of poly(4-aminophenol) modified graphite electrode as material for the immobilization of acetylcholinesterase were investigated by the Cyclic Voltammetry, Electrochemical Impedance Spectroscopy and Atomic Force Microscopy. The polymer was deposited on graphite electrode surface by the oxidation of 4-aminophenol and then acetylcholinesterase was immobilized on the surface of the electrode. The biosensor coupled in the continuous flow system was employed for the detection of dichlorvos. The detection and quantification limits were 0.8 and 2.4 μmol L-1 dichlorvos, respectively. Graphite electrodes modified with the poly(4-aminophenol) showed to be an efficient and promising material for immobilization of acetylcholinesterase enzyme. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Graphite; Poly(4-aminophenol); Biosensor; Dichlorvos.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600018

Physical-chemical characteristics and fatty acids composition in dairy goat milk in response to roughage diet
Provedor de dados: 52
Autores: Torii,Marcelo Shizuo; Damasceno,Júlio César; Ribeiro,Luciano da Rocha; Sakaguti,Eduardo Shiguero; Santos,Geraldo Tadeu dos; Matsushita,Makoto; Fukumoto,Nelson Massaru.
The objective of the present work was to evaluate the physical-chemical characteristics (density, pH, acidity, fat, protein, lactose and total of solids contents) and milk fatty acids composition (C: 4 to C: 20) in response to roughage sources (alfalfa hay T1; oat hay T2 and maize silage T3). Nine Saanen lactating goats were used, in a triple Youden square design (3 animals x 2 periods). There was no treatment effects in the physical-chemical variables in the univariate analyses; by multivariate analyses three distinct patterns of fatty acids could be defined: milk with greater quantity of short chain fatty acids and acids C17:1omega7 e C18:2omega6 (T1); milk with equivalent amounts of short, medium and long chain fatty acids (T2); and milk with greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alfafa hay; Maize silage; Milk quality; Milk composition; Oat hay; Saanen.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600010

First record of epibionts peritrichids and suctorians (Protozoa, Ciliophora) on Pomacea lineata (Spix, 1827)
Provedor de dados: 52
Autores: Dias,Roberto Júnio Pedroso; D'Ávila,Sthefane; D'Agosto,Marta.
This study made a survey on the ciliate protozoans from Peritrichid and Suctorian taxa, epibionts of Pomacea lineata (Spix, 1827) (Ampullariidae). Fifty mollusks were collected in places of irrigation ditches and shell was scraped with a scalpel and the extracted material was analyzed in vivo by light microscopy. All examined mollusks presented epibiont ciliates on their shells. For the first time epibiont ciliates in P. lineata was record. Seven genera of ciliates: five peritrichids and two suctorians, were identified and classified within the subclass Peritrichia: Carchesium Ehrenberg, 1838 (Vorticellidae), Epistylis Ehrenberg, 1830 (Epistylidae), Opercularia Stein, 1854 (Operculariidae), Vaginicola Lamarck, 1816 (Vaginicolidae) and Vorticella Linnaeus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epizoic ciliates; Prosobranch snail; Freshwater environment.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600015

Energy Analysis in Supermarkets
Provedor de dados: 52
Autores: Carvalho,Guilherme A.; Rohr,Michel; Bauer,Paulo Cezar; Betini,Roberto Cesar.
ABSTRACT This study shows, through several analyses, ways to reduce costs with electric power in supermarkets. For this purpose, one of those establishments was visited and several data were collected for subsequent analysis. The results have shown that the combinations of several actions of energy efficiency can be applied when the objective is the reduction of costs with electric power.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy efficiency; Analyses; Reduction of Tariff of Electric power; Supermarkets.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200228

Effects of Vitamin K1 Supplementation on the Risk Factors to the Stroke and on Memory in Spontaneously Hypertensive Rats Stroke Prone (SHR-sp)
Provedor de dados: 52
Autores: Cavargere,Victor Agati; Guimarães,Marcela Rodrigues Moreira; Murad,Leonardo Borges; Chaves,Amanda; Vianna,Lucia Marques.
A number of risk factors have been associated to the stroke and many strategies have been proposed in order to control them as well. Vitamin K has been largely found in brain, which suggests a possible function at that tissue. This study aimed to evaluate the potential of this vitamin on the prevention of risk factors to stroke and on cognitive function on SHRSP rats. Twelve SHRSP males, 15 weeks old, were divided into two groups (n= 6 each), receiving the vehicle-coconut oil (control group) or 40 μg of phylloquinone (treated group) during 28 days. Biological parameters, systolic blood pressure and lipid profile were evaluated. Both groups were submitted to the neurological tasks. The data was treated by Student's t test and ANOVA one-way test being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylloquinone; Memory; Stroke; SHRSP.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100313

Temporal dynamics of stomatal conductance of plants under water deficit: can homeostasis be improved by more complex dynamics?
Provedor de dados: 52
Autores: Souza,Gustavo Maia; Oliveira,Ricardo Ferraz de; Cardoso,Victor José Mendes.
In this study we hypothesized that chaotic or complex behavior of stomatal conductance could improve plant homeostasis after water deficit. Stomatal conductance of sunflower and sugar beet leaves was measured in plants grown either daily irrigation or under water deficit using an infrared gas analyzer. All measurements were performed under controlled environmental conditions. In order to measure a consistent time series, data were scored with time intervals of 20s during 6h. Lyapunov exponents, fractal dimensions, KS entropy and relative LZ complexity were calculated. Stomatal conductance in both irrigated and non-irrigated plants was chaotic-like. Plants under water deficit showed a trend to a more complex behaviour, mainly in sunflower that showed better...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta vulgaris; Complexity; Helianthus annus; Stability; Time series analyses; Water deficit.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300013

Reviewing Microbial Behaviors in Ecosystems Leading to a Natural Quorum Quenching Occurrence
Provedor de dados: 52
Autores: Zapata,Laura Sierra; Tabarez,Magally Romero; Álvarez,Javier Correa; Escobar,Valeska Villegas.
ABSTRACT Quorum sensing is considered one of the most important discoveries in cell-to-cell communication. Although revealed in Bacteria, it has been identified as well as a mechanism present in the other two domains, Eukaryota and Archaea. This phenomenon consists mainly of an exchange and sensing of "words" produced by each cell: chemical signals known as autoinducers. The process takes places at high cell densities and confined environments, triggering the expression of specific genes that manifest in a determined phenotype. Quorum sensing has a fundamental importance in the organisms' fitness in natural ecosystems since it activates many of the traits needed by cells to survive under specific conditions, and thus a wide variety of chemical signals,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quorum Sensing; Autoinducers; Interference; Degradation; Microbial Ecology; Quorum Quenching.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100409

Impact of biocatalyst and moisture content on toluene/xylene mixture biofiltration
Provedor de dados: 52
Autores: Klapková,Eva; Halecký,Martin; Fitch,Mark; Soccol,Carlos Ricardo; Paca,Jan.
The objective of this work was to determine the influence of microbial inoculation on degradation efficiency. Three biofilters were used for the treatment of waste gas. A mixture of compost and perlite (8:2) served as the packing material. One biofilter was inoculated with a constructed microbial population. The second remained uninoculated, having the natural population present in the compost. The third biofilter was uninoculated and the packing material was sterilized. The degradation ability of the uninoculated biofilter started to drop after 18 days, while the removal efficiency of inoculated biofilter was stable. The sterile biofilter proved to have no removal efficiency. Moisture content of the packing and ability of the packing to keep moisture was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofiltration; Constructed mixed population; Moisture content; Pollutant mixture.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300020

Sugar cane juice fermentation by Zymomonas mobilis CP4 subjected to inhibition by ethanol and high initial concentration of substrate
Provedor de dados: 52
Autores: Tano,Marcia S.; Buzato,João B.; Celligoi,Maria Antonia P. C.
Sugar cane juice fermentation by Z. mobilis CP4 subject to stress by ethanol and high concentration of sucrose was investigated. Supplementation with saponifiable portion of soybean oil deodourising destillate (SOD) at 5; 10 and 15 mL/L.The ethanol production resulted values of 15.91; 16.99 and 15.63 g/L respectively. Values of Yp/s 0.35; 0.36 and 0.37 were achieved, which represented 20.69; 24.14 and 27.59% respectively higher when unsupplemented medium fermentation was carried out.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymomonas mobilis; Fermentation; Lipids; Ethanol.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400012

Isolation and Identification of a Potential Amylolytic Probiotic Bacterium from the Gut of Jundiá Catfish, Rhamdia quelen.
Provedor de dados: 52
Autores: Poletto,Tatiana Vieira; Vieira,Cleide Rosana Werneck; Silva,Carlos Peres; Fracalossi,Debora Machado.
ABSTRACT This study aimed to isolate a potential probiotic amylolytic strain from the gut of jundiá catfish to improve carbohydrate digestibility in fish. Two of 31 strains isolated from the foregut of Rhamdia quelen were able to grow on starch-agar medium and were considered amylolytic. The strain that presented higher amylolytic potential, based on a qualitative amylase assay, was chosen. The strain was phenotypically characterized and analysed to determine bile and pH tolerance and extracellular quantitative amylase activity. The probiotic candidate, identified as Aeromonas veronii, showed the ability to survive stresses from a range of pH and bile salt conditions and secreted an interesting enzymatic profile, which may exhibit a synergistic effect when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Probiotics; Aquaculture; Intestinal microbiology; Aeromonas veronii.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100323

Determination of N-nitrosamines and N-nitrosables substances in rubber teats and sothers by GC-TEA
Provedor de dados: 52
Autores: Vieira,Eleno Rodrigues; Pierozan,Nilso José; Lovison,Viviane.
N-Nitrosamines are receiving special attention because they present high mutagenic and carcinogenic potential. They can be found in several areas. In rubber, for example, they can be found in Nipples and Pacifiers. Spiegelhalder and Pressumann, have observed that N-Nitrosamines present in nipples and pacifiers could easily migrate to artificial saliva. They have also observed that a considerable amount of N-Nitrosamines precursors, such as nitrosable amines, could migrate to saliva, increasing the possibility of N-Nitrosamines formation into the stomach of living beings. In 80's, Holland, Germany and FDA-U.S.A. have established volatile N-Nitrosamines limit levels. In 2002, in Brazil, limit levels came through Resolução RDC n° 221 of ANVISA. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-Nitrosamines; Method; Nipples and pacifiers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200012

Decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigatus
Provedor de dados: 52
Autores: Kalaiarasi,K.; Lavanya,A.; Amsamani,S.; Bagyalakshmi,G.
The aim of this work was to study the decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigates. The dried non-viable fungal biomass exhibited maximum dye removal at pH 7.0 with temperature of 30ºC and 3 g/l (w/v) biomass concentration, after 24 h contact time. The results showed that the non-viable biomass possessed high efficiency for dye removal from textile effluent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Decolourization; Textile effluent.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300019

Physicochemical properties of starches from two different yam (Dioscorea opposita Thunb.) residues
Provedor de dados: 52
Autores: Wang,Yugao; Zhang,Liming; Li,Xinglin; Gao,Wenyuan.
The starches obtained from two different yam residues, which were treated with alkali(starch-A) or enzyme (starch-E), were studied and compared with yam starch isolated using ordinary method (starch-O) for morphological, crystalline pattern, thermal, and pasting properties. The results revealed that the amylose content of three starches ranged from 19.47 to 22.17%. The granule surfaces of starch-A and starch-E were as smooth as that of starch-O. The crystalline pattern of the three starches was a C-type. The transition temperatures (To, Tp and Tc) varied from 70.11 to 73.64, 79.23 to 81.74, and 84.30 to 86.65 ºC, respectively. The starch-E showed the highest Δ Hgel, followed by the starch-A, while it was lowest for the starch-O. According to the viscosity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yam starch; Yam residue; Thermal; Crystallinity; Pasting property.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200004

Characterization of Some Efficient Cellulase Producing Bacteria Isolated from Pulp and Paper Mill Effluent Contaminated Soil
Provedor de dados: 52
Autores: Reddy,Kondakindi Venkateswar; Vijayalashmi,Tatiparti; Ranjit,Pabbati; Raju,Maddela Naga.
ABSTRACT The wide variety of bacteria in the environment permits screening for more efficient cellulases to help overcome current challenges in biofuels production. This study focuses on the isolation of efficient cellulase producing bacteria found in pulp and paper mill effluent contaminated soil which can be considered for use in large scale biorefining. Four different bacterial strains were isolated and screened for cellulase production by using CMC agar medium. All isolates showed cellulase activity these strains were further characterized by morphological, physiological, biochemical and 16S rRNA gene analyses. These isolates were identified as two Bacillus subtilis sub sps, Bacillus mojavensis and Bacillus cereus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CMC Agar; Cellulolytic Bacteria; Molecular identification; 16S rRNA; Cellulolytic activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100442

Storage sites in seeds of Caesalpinia echinata and C. ferrea (Leguminosae) with considerations on nutrients flow
Provedor de dados: 52
Autores: Teixeira,Simone de Pádua; Machado,Sílvia Rodrigues.
The seeds of Caesalpinia echinata and C. ferrea behaved as typical endospermic seeds, despite their different morphological classification (exendospermic seeds were described for C. echinata and endospermic seeds for C. ferrea). Then, the aim of this work was to compare, under ultrastructural and histochemical terms, the nature of the storage substances and their accumulation sites, as well as the nutrient flow in seeds of these species. Cotyledons in C. echinata accumulate carbohydrates, lipids and proteins, which are mobilized from the outer to the inner parts as revealed by the position of plasmodesmata. Endosperm in C. ferrea accumulates carbohydrates and in C. echinata accumulates substances during the initial embryogenic phases. Such tissue develops...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotyledon; Embryo; Endosperm; Histochemistry; Reserve substances; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100016

Colonization of artificial substrata by teredinid larvae released from a previously infested focus at Ilha Grande Bay, RJ
Provedor de dados: 52
Autores: Varotto,Ricardo Silva; Barreto,Cristine Costa.
The colonization pattern of wooden panels by teredinid larvae released from a previously infested focus was investigated. Panels were placed at different distances (1, 10, 20m) and in different directions from the focus. The colonization experiment took place at Parcel do Aleijado, Ilha Grande Bay, RJ. The focus remained immersed in nearly surface water, by the coast, during three months for infestation by teredinids. After this period, it was transferred to the study site. The infested focus and colonization panels remained in water, 10m deep, for three months more. Total density and density of the dominant species were considered for each group of panels. Five teredinid species were found: Bankia fimbriatula, Bankia gouldi, Lyrodus floridanus, Lyrodus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teredinidae; Colonization; Benthic ecology; Dispersion.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400002

Absorbed dose measurements using TLDS in biological samples from beta radiation
Provedor de dados: 52
Autores: Manzoli,José Eduardo; Carvalho,Valdir de Souza; Cerqueira,Eliomar Jacinto Gomes de.
Irradiation of samples in peculiar experimental apparatus, subject to radiation spread, requires a special evaluation of absorbed dose implanted to the sample. Indirect calibration of the irradiation source, obtained in a different apparatus, and the spread, usually of very difficult theoretical evaluation, can cause very serious measurement errors, sometimes reaching 50%. In this work, the procedure for dose evaluation in an apparatus for beta irradiation of samples, usually biological ones,is presented, making use of calibration curves, obtained by irradiation in advance of thermoluminescent detectors in air, and so irradiating them in the same position of the sample. An application in blood sample irradiation is also presented.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta irradiation; Beta dosimetry; Thermoluminescence; Biological irradiation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200008

Recent developments on broodstock maturation and reproduction of Farfantepenaeus paulensis
Provedor de dados: 52
Autores: Peixoto,Sílvio; Cavalli,Ronaldo Olivera; Wasielesky,Wilson.
This paper describes the latest developments on maturation and reproduction of Farfantepenaeus paulensis in captivity, with special reference to the studies conducted during the last decade at the Laboratory of Mariculture, University of Rio Grande in southern Brazil, aiming to improve the future outlook for a sustainable nauplii production of F. paulensis. Particular emphasis has been given on the reproductive cycle, ovarian maturation, maturation and spawning systems, artificial insemination, broodstock reproductive performance and maintenance of captive broodstock.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproduction; Maturation; Farfantepenaeus paulensis; Penaeid.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800016

Nuclear medicine methods in the assessment of acupuncture effects: a short review
Provedor de dados: 52
Autores: Souza,Deise Elisabete; Rebello,Bernardo Machado; Silva Filho,Reginaldo de Carvalho; Agostinho,Raquel Terra; Bastos,Sohaku R. C.; Bernardo-Filho,Mario.
The mechanisms of acupuncture are poorly understood. In consequence, numerous investigators have conducted clinical trials to test the efficacy of acupuncture in various conditions. We have used PubMed database system to evaluate the number of publications in acupuncture and nuclear medicine procedures in the period from 1964 to 2007, using the keywords: "nuclear medicine and acupuncture", "SPECT and acupuncture", "PET and acupuncture", "scintigraphy and acupuncture", "radionuclide and acupuncture", "radiopharmaceutical and acupuncture", "radioisotope and acupuncture" and "99mTc and acupuncture". Some papers published in English language were selected and a short review is presented The analysis of the number of publications shows that when a method is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; Nuclear medicine; Traditional Chinese Medicine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600012

Effects of simulated acid rain on leaf anatomy and micromorphology of Genipa americana L. (Rubiaceae)
Provedor de dados: 52
Autores: Sant'Anna-Santos,Bruno Francisco; Silva,Luzimar Campos da; Azevedo,Aristéa Alves; Aguiar,Rosane.
Experiments were conducted in order to characterize the injuries on leaf structure and micromorphology of G. americana and evaluate the degree of susceptibility of this species to simulated acid rain. Plants were exposed to acid rain (pH 3.0) for ten consecutive days. Control plants were submitted only to distilled water (pH 6.0). Leaf tissue was sampled and fixed for light and scanning electron microscopy. Necrotic interveinal spots on the leaf blade occurred. Epidermis and mesophyll cells collapse, hypertrophy of spongy parenchyma cells, accumulation of phenolic compounds and starch grains were observed in leaves exposed to acid rain. The micromorphological analysis showed, in necrotic areas, plasmolized guard cells and cuticle rupture. Epidermal and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genipa americana; Simulated acid rain; Leaf anatomy; Leaf micromorphology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300017

Gastrintestinal helminths of capybara (Hydrochoerus hydrochaeris, Linnaeus, 1766) in Cattle Breeding Farm in the area of the Ecological Reserve of Taim, Rio Grande
Provedor de dados: 52
Autores: Sinkoc,Afonso Lodovico; Brum,João Guilherme Werner; Muller,Gertrud.
The aim of this study was we observe the prevalence, intensity and mean intensity of theinfection by gastrintestinal helminths in capybaras. The helminths collected resulted in the following relationship with their respective prevalence and mean intensities of infection: Strongyloides sp. - 62.55% and 168.67; Protozoophaga obesa - 95.83% and 2698.26; Vianella hydrochoeri - 70.83% and 83.82; Hydrochoerisnema anomalobursata - 58.33% and 57.86; Capillaria hydrochoeri - 83.33% and 35.25; Trichuris spp. - 50.00% and 36.58; Hippocrepis hippocrepis - 62.50% and 22.40; Taxorchis schistocotyle - 12.50% and 2.33; Hydrochoeristrema cabrali - 45.83% and 10.73; Monoecocestus hydrochoeri - 50,00% and 28.25 and Monoecocestus jacobi - 41.67% and 5.40. The helminths M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrochoerus hydrochaeris; Helminths; Capybara.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200009

Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity
Provedor de dados: 52
Autores: Singariya,Premlata; Kumar,Padma; Mourya,Krishan Kumar.
The present study was carried out to determine the possible bioactive components (steroids) of Cenchrus setigerus using GC-MS analysis and in vivo estimation of metabolites (total soluble sugar, soluble protein, proline and total phenolics), photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) of seedlings and antimicrobial activity of extracts in various polar solvents from the leaves of C. setigerus. Antimicrobial activity was evaluated against three Gram-negative bacteria, including Proteusmirabilis, Klebsiella pneumonia and Agrobacterium tumefaciens andone fungus Aspergillus niger using 'disc diffusion' method, followed by the determination of minimum inhibitory concentrations (MIC) by broth dilution method. Results revealed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cenchrus grass; Photosynthetic pigments; Total soluble sugar; Proline; Antibacterial activity; MIC; MBC/MFC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100010

Performance of nymphs and adults of Piezodorus guildinii (Westwood) (Hemiptera: Pentatomidae) on soybean pods at different developmental stages
Provedor de dados: 52
Autores: Oliveira,Émerson D. M.; Panizzi,Antônio R.
A study was conducted in the laboratory to test the suitability of soybean [Glycine max (L.) Merrill] pods at different phenological stages of development (R3-R8) on the performance of nymphs and adults of the small green stink bug Piezodorus guildinii (Westwood) (Hemiptera: Pentatomidae). Nymphs and adults showed better performance when fed on soybean pods during the pod-filling stage (R5-R6), compared to those fed on soybean pods at the remaining stages of development. When fed on soybean pods without seeds (R3-R4) no nymphs survived and no adults reproduced.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heteroptera; Pentatomidae; Stink bug; Glycine max; Soybean pod phenology; Biology.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200008

A new alternative to produce gibberellic acid by solid state fermentation
Provedor de dados: 52
Autores: Rodrigues,Cristine; Vandenberghe,Luciana Porto de Souza; Teodoro,Juliana; Oss,Juliana Fraron; Pandey,Ashok; Soccol,Carlos Ricardo.
Gibberellic acid (GA3) is an important hormone, which controls plant's growth and development. Solid State Fermentation (SSF) allows the use of agro-industrial residues reducing the production costs. The screening of strains (four of Gibberella fujikuoroi and one of Fusarium moniliforme) and substrates (citric pulp, soy bran, sugarcane bagasse, soy husk, cassava bagasse and coffee husk) and inoculum preparation study were conducted in order to evaluate the best conditions to produce GA3 by SSF. Fermentation assays were carried out in erlenmeyers flasks at 29°C, with initial moisture of 75-80%. Different medium for inoculum production were tested in relation to cells viability and GA3 production by SSF. F. moniliforme LPB 03 and citric pulp were chosen for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gibberellic acid; Fusarium moniliforme; Solid state fermentation; Inoculum; Agro-industrial residues.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700023

Cultivation of native seaweed Gracilaria domingensis (Rhodophyta) in Southern Brazil
Provedor de dados: 52
Autores: Salles,José Pedrassoli; Scherner,Fernando; Yoshimura,Cristalina Yoshie; Fanganiello,Maurício; Bouzon,Zenilda Laurita; Horta,Paulo Antunes.
The aim of this work was to study the cultivation of Gracilaria domingensis in a mussel farming urbanized area in Santa Catarina, Brazil. Relative growth rate was the parameter used to evaluate the cuttings attachment methods on the cultivation rope, cuttings density, cultivation period and cystocarpic versus unfertile thalli performance. The cultivation was feasible only when protected by net cages due to herbivory. The tie-tie attachment method presented the best results. No differences were observed when comparing the cuttings densities and reproductive phase. Future studies should evaluate the cost-effectiveness of producing the species in net cages and its potential as biofilter.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gracilaria domingensis; Marine aquaculture; Seaweed cultivation; Cultivation methods.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300018

Cotton zoning based on sowing periods of lower risk in Parana state, Brazil
Provedor de dados: 52
Autores: Wrege,Marcos Silveira; Caramori,Paulo Henrique; Gonçalves,Sergio Luiz; Almeida,Wilson Paes de; Marur,Celso Jamil; Pires,José Ricoy; Yamaoka,Ruy Seiji.
Cotton is cultivated in the North and West of Parana State, southern Brazil, under conditions of climatic risk variable in space and time. Risks of temperature below 15ºC at the establishment period, daily average temperature below 20ºC at the stage of cotton boll opening, and soil water deficit for both plant establishment and flowering periods, were estimated to identify homogeneous zones with sowing periods of lower climatic risk. The time interval with adequate temperature, associated with minimum risks of the other factors and yield data from field experiments allowed the identification of seven distinct zones, with best sowing periods ranging from September 20 to November 20. Official credit to the farmers is conditioned by the Central Bank of Brazil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotton; Climatic risk; Planting dates; Zoning; Low temperature.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100010

Evaluation of different media for the production of cephalosporins by Streptomyces clavuligerus ATCC 27064
Provedor de dados: 52
Autores: Antonio,Tatiana; Bellão,Carolina; Corrêa,Tatiana; Cavallieri,André Pastrelo; Badino,Alberto Colli; Araujo,Maria Lucia Gonsales da Costa.
The aim of this work was to compare the composition of a complex and soluble culture medium to eight other media described in the literature through the batch cultivation in a conventional bench-scale bioreactor for the production of cephamycin C by a wild strain of Streptomyces clavuligerus. The proposed medium resulted in an antibiotic production 1.5 to 7.5 times higher than the other culture media.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-lactam antibiotic; Streptomyces clavuligerus; Lysine metabolism; Complex medium.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600003

Osteoarthritis in horses - Part 1: relationship between clinical and radiographic examination for the diagnosis
Provedor de dados: 52
Autores: Souza,Maria Verônica de.
ABSTRACT Locomotor disorders are very common in the equine clinic, which may be partly due to the different types of activities horses develop. Osteoarthritis (OA), commonly known as degenerative joint disease, presents an considerable role in the series of disorders of the musculoskeletal system and may be associated with other problems such as navicular syndrome, periostitis or osteochondrosis. This affection causes progressive deterioration of articular cartilage, accompanied by bone and soft-tissue periarticular changes. In fact, it results from a complex interaction between biochemical and biomechanical factors. The objective of this article is to review information about clinical and radiographic findings of OA, the biochemical and biomechanical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Degenerative joint disease; Synovitis; Cartilage degeneration; Pathology of articular disease.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100325

Histopathologic changes in the kidney tissue of Prochilodus lineatus Valenciennes, 1836 (Characiformes, Prochilodontidae) induced by sublethal concentration of Trichlorfon exposure
Provedor de dados: 52
Autores: Veiga,Marcelo Leite da; Rodrigues,Edson de Lara; Pacheco,Fabio Juliano; Ranzani-Paiva,Maria José Tavares.
Studies were carried out to analyse the kidney histopathological alterations of "curimbatá", Prochilodus lineatus, were analyzed. An acute bioassay was made by the water contamination with 0.2muml/l of Dipterex 500 (Trichlorfon). The kidney tissue collected after 24 hours of exposure showed an enlargement of intercapsular space with glomerular atrophy, hypertrophy of the kidney tube cells, with small granules on its cytoplasm and little nuclear alteration. Blood overflowing from capillaries with pyknotic nuclei and vacuoles in the cytoplasm it was also noticed. After 48 hours, the kidney tissue showed glomerular expansion, impossibility to visualize the intercapsular space as well as cytoplasm limit of many cells. The parietal capsular epithelium and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney; Prochilodus lineatus; Curimbatá; Trichlorfon.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200007

Influence of cosmetics vehicles on 4-methylbenzylidene-camphor's skin penetration, in vitro
Provedor de dados: 52
Autores: Sasson,Clarice Scliar; Sato,Mayumi Eliza; Beletti,Klézia da Silva; Mota,Fabrício Cunha; Piaceski,Angela Dakiw.
The aim of this work was to compare the skin penetration of 4-methylbenzylidene-camphor (4-MBC) in two vehicles, an oil-in-water (O/W) emulsion and an alcoholic gel. The penetration of this UVB filter through these vehicles was determined in vitro (Franz cells) using pig ear skin. The 4-MBC permeated through the skin both with the emulsion o/w as the alcoholic gel. However, with the alcoholic gel, 5 h after application of the product, the 4-MBC was found in the receptor fluid, while with the emulsion o/w, 24 h after it was detected. In both vehicles, the 4-MBC was present in the viable epidermis, dermis but most part of it, was found in the stratum corneum, being more remarkable for alcoholic gel. The right choice of the vehicle could decrease the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-Methylbenzylidene-camphor; Sunscreens; Penetration; Vehicles.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200006

Determination of yeast killer activity in fermenting sugarcane juice using selected ethanol-making strains
Provedor de dados: 52
Autores: Ceccato-Antonini,Sandra Regina; Tosta,Christiann Davis; Silva,Ana Cláudia da.
Twenty-four yeasts out of 342 isolated from the fermentative process showed killer activity and three of them were selected for the fermentative efficiency evaluation in batch system with cell recycle, flask and fermentor experiments. The selected three killer strains did not present similar results to those of pressed (baking) yeast concerning ethanol (0.07-0.18; 0.12-0.20; 0.10-0.13; 0.22-0.25 g/g, respectively) and biomass (0.19-0.26; 0.33-0.39; 0.13-0.27; 0.47-0.61 g/g, respectively) yields and fermentative efficiency (12.3-36.3; 21.0-40.0; 19.3-26.3; 47.6-54.0 %, respectively) in sugarcane juice, in flasks. In fermentor, similar behaviour was observed. However, the selected strains showed high cellular viability and killer activity (using cell-free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Killer yeasts; Alcoholic fermentation; Ethanol; Killer toxin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100003

Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation
Provedor de dados: 52
Autores: Slivinski,Christiane Trevisan; Machado,Alex Vinicius Lopes; Iulek,Jorge; Ayub,Ricardo Antônio; Almeida,Mareci Mendes de.
In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucoamylase; Aspergillus niger; Solid-state fermentation; Biochemical characterization; Purification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018

Turkey Meat. Seasonal Effect on Meat Quality and on Dead on Arrival Index in a Commercial Plant
Provedor de dados: 52
Autores: Carvalho,Rafael Humberto de; Soares,Adriana Lourenço; Guarnieri,Paulo Donizete; Oba,Alexandre; Ida,Elza Iouko; Shimokomaki,Massami.
ABSTRACT The objective of this study was to determine the incidence of Pale, Soft, Exudative (PSE) meat and to characterize the Dark, Fim, Dry (DFD) meat as well as the Dead on Arrival (DOA) index in a commercial turkey slaughterhouse in southern Brazil during the summer and winter seasons. The journeys (n=64) were over a distance of 36 ± 20 km and took approximately 95 ± 20 min. Color (L*) and pH distribution of turkey breast meat (n=5,352) were evaluated from different farms (n=64) during the 2015-2016 years. The pH, water-holding capacity, color (L*, a*, b*) were used to establish cutoff values for DFD meat (L* &lt; 44.0 and pH &gt; 5.90) and presented correlation among them. The L* value was the highest during the summer (P&lt;0.01), indicating the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal Welfare; Color Meat; DFD meat; PSE meat; Slaughterhouse.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100505

Land reclamation recovery with the sewage sludge use
Provedor de dados: 52
Autores: Tamanini,Cristina Rincon; Motta,Antônio Carlos Vargas; Andreoli,Cleverson Vitório; Doetzer,Benno Henrique.
In this work, investigations were carried out with five treatments [control, three doses of lime stabilized sludge (60, 120, 240 Mg ha-1 dry base) and soil corrective plus mineral fertilizer] to evaluate the immediate recuperation of a borrowed area. The application of stabilized alkaline sewage sludge acted as an acidity corrective, allowed the increase in the organic matter contents (21 to 43.5g dm-3) and available P (44 to 156 mg dm-3). Even with the use of the highest dose, no increase in the concentration of 32 analyzed metals was observed, due to the low concentration of metals in the sludge. The experiment showed that short term restoration of degraded area was possible by using high rates of sewage sludge without metal contamination.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sewage sludge; Land reclamation; Soil fertility; Soil chemistry; Degraded area.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400023

Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
Provedor de dados: 52
Autores: Zhang,Wen; Ren,Xiaohui; Bao,Liyin.
ABSTRACT Alginate is a major component of brown algae, but it cannot be utilized for ethanol fermentation by industrial microorganisms. A natural alginate degrading and ethanol producing strain was obtained in our previous research. However, the research on the ethanol metabolism process of the natural alginate fermentation strain is lacked. In this research, the key enzyme and metabolic process of ethanol fermentation were studied. Three kinds of key enzyme including alginate lyase, pyruvate dehydrogenase and ethanol dehydrogenase were determined. The enzyme activity in the metabolic process was relatively high at 60-96 h which was the most important period during the fermentation. Meanwhile the concentration change of the important substances including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alginate fermentation strain; Brown algae; Ethanol metabolism process.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100201

Cuticle of 'Gala' and 'Galaxy' apples cultivars under different environmental conditions
Provedor de dados: 52
Autores: Tessmer,Magda Andréia; Antoniolli,Lucimara Rogéria; Appezzato-da-Glória,Beatriz.
This study aimed to analyze the cuticle thickness and pattern of epicuticular wax deposition in 'Gala' and 'Galaxy' apple cultivars (Malus domestica Borkh,) from three Brazilian producing areas: Vacaria (RS), Fraiburgo (SC) and São Joaquim (SC) with altitudes of 971, 1,048 and 1,353m, respectively. Harvested fruit were kept under two storage conditions: regular atmosphere (RA) (0 ºC and 90% RH) and controlled atmosphere (CA) (1.5% O2, 2.5% CO2, 0ºC and 90% RH). Cuticle thickness measurements were made using LM and the deposition pattern of epicuticular wax observed with a SEM. Altitude among the apple producing areas was not a factor in deposition pattern of waxes between the cultivars but at higher altitudes, the cuticle was thicker in both the cultivars....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malus domestica Borkh; Altitude; Cold storage; Controlled atmosphere; Light microscope.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500010

Effects of Oral Oxytetracycline-Therapy on Wound Progression and Healing Following Aeromonas caviae Infection in Nile Tilapia (Oreochromis niloticus L.)
Provedor de dados: 52
Autores: Roy,Anwesha; Abraham,Thangapalam Jawahar; Namdeo,Meshram Supradhnya; Singha,Jasmine; Julinta,Roy Beryl; Boda,Satyanarayana.
Abstract The effects of oral oxytetracycline (OTC)-therapy against Aeromonas caviae infection as well as the wound progression and healing in intramuscular (IM) and abrasion-immersion (AI) challenged Nile tilapia juveniles were evaluated. The IM challenge caused significantly (p &lt; 0.05) high mortalities (90%) compared to AI challenge (40%). The mortalities recorded in 10 days OTC-fed (72% in IM group and 30% in AI group) and untreated Nile tilapia were significantly (p &lt; 0.05) high compared to positive (5-10%) controls. The reduction in mortalities in OTC-fed Nile tilapia was significant (p &lt; 0.05) with no further mortalities during the post-OTC therapy period. In IM group, the black scar disappearance, re-growth of dermal fibrous tissue and skin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas infection; Abrasion-immersion; Intramuscular injection; Antibiotic therapy; Medicated feed.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100317

Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems
Provedor de dados: 52
Autores: Reali,Catiusca; Pilz Júnior,Harry Luiz; Meile,Jean Christophe; Galindo,Sabine Shorr; Fiuza,Lidia Mariana.
ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agroecosystems; Functional genes; Bacteria; DGGE; Metagenomics; Microbial ecology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100201

Morphology and Anatomy of the Flowers of Trichilia catigua A. Juss., T. elegans A. Juss. and T. pallida Sw. (Meliaceae)
Provedor de dados: 52
Autores: Souza,Luiz A. de; Moscheta,Ismar S.; Mourão,Káthia S. M.; Silvério,Adriano.
Morphological and structural features of flowers of Trichilia catigua A. Juss., T. elegans A. Juss. and T. pallida Sw. (Meliaceae) are presented here with the purpose to stimulate future reproductive and preservation studies of these brazilian native species. Anthesis occurred from April to August in T. catigua, January to May in T. pallida and September to January in T. elegans. Sepals and petals presented a papillose and pilose epidermis and a parenchymatous mesophyll. Male flowers presented pistillodes with abortive ovules and a staminal tube with tetrasporangiate anthers. Anther wall had a papilose epidermis, a fibrous endothecium, two middle layers and a secretory tapetum. Female flowers presented antherodes and bi - or tricarpellate syncarpous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichilia; Meliaceae; Floral morphology and anatomy; Flowering.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400008

BMP4 Induces Cardiomyocyte Hypertrophy Through the Activation of ERK 1/2 Signaling Pathway in H9c2 Cells
Provedor de dados: 52
Autores: Yuan,Yu; Tao,Yezheng; Deng,Yongzhi; Ye,Qunhui; Lin,Bin; Wu,Lin.
Abstract Bone morphogenetic protein-4 (BMP4) is a member of the bone morphogenetic protein family which plays an important role in bone formation, inflammation and cardiac hypertrophy. The aim of this study was to investigate the underlying molecular mechanism that BMP4-induced cardiomyocyte hypertrophy. H9c2 cells were used to measure cell surface area and protein synthesis. Western blot was used to examine hypertrophic marker brain natriuretic peptide (BNP) protein expression and phosphorylation of ERK1/2. The results exhibited that cell surface area, protein synthesis and BNP protein expression were increased with BMP4 treatment. While PD98059 inhibited these effects of BMP4. In addition, BMP4 treatment increased phosphorylation of ERK1/2 in a time- and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BMP4; Myocardial hypertrophy; ERK1/2 signaling pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100315

Production of Hydrolysate of Okara Protein Concentrate with High Antioxidant Capacity and Aglycone Isoflavone Content
Provedor de dados: 52
Autores: Figueiredo,Vitória Ribeiro Garcia de; Justus,Ariana; Pereira,Dafne Garcia; Georgetti,Sandra Regina; Ida,Elza Iouko; Kurozawa,Louise Emy.
Abstract This work aimed to evaluate the enzymatic hydrolysis of okara protein concentrate with respect to degree of hydrolysis (DH) in order to obtain a protein hydrolysate with high antioxidant capacity and aglycones isoflavone content. A central composite rotatable design was carried out to evaluate the influence of temperature (40 to 70°C), enzyme:substrate ratio (0.5 to 5.0%, g/100g protein) and pH (7.0 to 9.0) on DH. The optimal condition was 55°C, pH 9 and enzyme:substrate ratio of 5.0%, resulting a DH value of 35.5%. After protein hydrolysis at optimal condition, the antioxidant capacities of hydrolysate increased from 58.29 to 383.49 μM Trolox equivalent/g solids (ABTS method) and 2.41 to 15.32 μM Trolox equivalent/g solids (FRAP method) when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soy pulp; Peptides; Cleavage of protein; Isoflavone profile; Electrophoresis; Experimental design.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100505

Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
Provedor de dados: 52
Autores: Moreira,Mônica; Noschang,Juliana; Neiva,Ivana Froede; Carvalho,Yanê; Higuti,llma Hiroko; Vicente,Vânia Aparecida.
In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus; DNA isolation; CTAB; Ultrasound.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013

Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation
Provedor de dados: 52
Autores: Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud.
ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100436

Effect of Wnt/β-catenin and NF-κB signaling pathways on mucus secretion with hypertonicity in 16HBE cells
Provedor de dados: 52
Autores: Xiaoyan,Liu; Xiangdong,Zhou.
This study aimed at identifying the molecular mechanisms and effects of hypertonicity on mucin5AC (MUC5AC) expression in airway epithelial cells for which immortalized human bronchial epithelial (16HBE) cells were cultured in 600 mOsm/L hypertonic medium for different times in vitro. Proteins of MUC5AC and β-catenin, Cyclin D1, NF-κB p65 were detected by enzyme linked immunosorbent assay (ELISA), reverse transcription (RT)-PCR and western blotting assays. After transfection of β-catenin siRNA in 16HBE cells, the levels of β-catenin, Cyclin D1, NF-κB p65 and MUC5AC protein were detected. Results showed that the levels of mRNAs and proteins of target genes increased significantly after the exposure to hypertonic conditions and the expression content...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MUC5AC; Hypertonicity; Wnt/β-catenin; NF-κB p65.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400006

Assessment of oxidation of leaves of Ilex paraguariensis (St. Hil)
Provedor de dados: 52
Autores: Molin,Rochele Fernanda; Valduga,Alice Teresa; Di Luccio,Marco; Dartora,Nessana; Cichoski,Alexandre José; Pistore,Mônica; Rigo,Elisandra.
This work aimed to study the oxidation of leaves of Ilex paraguariensis St. Hil, in a process similar to the oxidation of Camellia sinensis, with the purpose of developing new products based on yerba maté. So far, no previous systematic study about the oxidation of yerba maté has been found elsewhere. A Plackett-Burman experimental design was used for the screening of the significant parameters that affected the oxidation. The oxidation was indirectly quantified by following the changes in the product color, water activity, and pH with the time of processing. The factors that most influenced the process were the age of the leaves and the relative humidity of the oxidation chamber. The differences between the treatments were more evident after 3, 6 12 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physicochemical parameters; Yerba maté; Leave; Extract; Experimental design.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200016

Erratum:Development of an L-Lysine Enriched Bran for Animal Nutrition via Submerged Fermentation by Corynebacterium glutamicum using Agroindustrial Substrates
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100905

Evaluation of Medicinal Values of Gymnopetalum chinense (Lour.) Merr., a Lesser Known Cucurbit from Eastern Ghats of India
Provedor de dados: 52
Autores: Kumar,Sanjeet; Das,Gitishree; Shin,Han-Seung; Kumar,Pradeep; Patra,Jayanta Kumar.
ABSTRACT &gt;The in vitro antimicrobial activity of Gymnopetulum chinense (Cucurbitaceae), a lesser known climber of Eastern Ghats and recently recorded species from Odisha, India was studied against five selected human pathogenic bacteria (Streptococcus mutans, S. pyogenes, Vibrio cholerae, Shigella flexneri and Salmonella typhi), by agar well diffusion (AWD), disc diffusion (DD) and broth dilution methods. Different solvent extracts were prepared from the leaves of G. chinense using various solvents viz. n-hexane, chloroform, methanol, acetone and aqueous. The nomenclature, detailed description and photographs of the plant are provided to facilitate its easy identification. The leaf extract showed presence of phenolic compounds, tannin, saponin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial activity; Gymnopetulum chinense; Cucurbits; Streptococcus pyogenes; Antimicrobial resistance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100438

Microbiological and physicochemical treatments applied to metallurgic industry aiming water reuse
Provedor de dados: 52
Autores: Bello,Antonio Roberto Crystal; Angelis,Dejanira de Franceschi de; Domingos,Roberto Naves.
A study was conducted on the reuse of the water in a system composed of a sewage treatment plant (STP) using prolonged aeration with activated sludge and a compact water treatment plant (CWTP) in a metallurgic industry. The processes for obtaining the water for reuse were microbiological and physicochemical. The domestic sewage was then pumped to the STP, where biological flocks were formed and clarified water was obtained. The efficiency of the microbiological process in the STP was evaluated for removal of biochemical oxygen demand (BOD), chemical oxygen demand (COD) and sedimentary solids (SS). The efficiency of physicochemical processes for clarifying the water and disinfection was evaluated through analysis of pH, turbidity, color, aerobic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water; Reuse; Sewage; Microorganisms; Disinfection.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200020

In Silico Characterization of Meloidogyne Genus Nematode Cellulose Binding Proteins
Provedor de dados: 52
Autores: Menezes,Alana Manoela Fraga; Cavalcanti Junior,Edilton de Albuquerque; Martins,Luiza Suely Semen; Moraes Filho,Rômulo Maciel de.
Abstract Root-knot nematodes are a group of endoparasites species that induce the formation of giant cells in the hosts, by which they guarantee their feeding and development. Meloidogyne species infect over 2000 plant species, and are highly destructive, causing damage to many crops around the world. M. enterolobii is considered the most aggressive species in tropical regions, such as Africa and South America. Phytonematodes are able to penetrate and migrate within plant tissues, establishing a sophisticated interaction with their hosts through parasitism factors, which include a series of cell wall degradation enzymes and plant cell modification. Among the parasitism factors documented in the M. enterolobii species, cellulose binding protein (CBP), a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Phytonematodes; Parasitism Factors; Cell wall degrading enzymes; Homology modeling; Protein function prediction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100201

Methane concentration in water column and in pore water of a coastal lagoon (Cabiúnas lagoon, Macaé, RJ, Brazil)
Provedor de dados: 52
Autores: Fonseca,André Luiz dos Santos; Minello,Murilo; Marinho,Claudio Cardoso; Esteves,Francisco de Assis.
The aim of this study was to evaluate methane concentration in water column and pore water at limnetic and littoral regions of a coastal lagoon. At the littoral region samples were taken from three monospecific macrophytes stands (Typha domingensis, Eleocharis interstincta and Potamogeton stenostachys). The methane concentration in the pore water at the littoral region was higher than the concentration found at the limnetic region in each fraction of the sediment. The higher methane concentration in the superficial fraction of the sediment (0-2 cm) was shown at the P. stenostachys stand (3.7 mM). It was the only significantly different (p&lt;0.05) from the limnetic region. The pore water methane concentration increased with depth at the aquatic macrophytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methane; Water column; Sediment; Aquatic macrophytes; Coastal lagoons.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200018

Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
Provedor de dados: 52
Autores: Tan,Dao Xuan; Tuong,Ho Manh; Thuy,Vu Thi Thu; Son,Le Van; Mau,Chu Hoang.
ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drought-resistant; GmDREB2 gene; Gene transfer; Soybean; Transcription factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651

Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
Provedor de dados: 52
Autores: Finokiet,Manuela; Goni,Beatriz; Loreto,Élgion Lúcio Silva.
Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable elements; Insect transgenesis; Germ-line transformation; Fluorescent protein marker; Microinjection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013

Horizontal transmission and effect of the temperature in pathogenicity of Beauveria bassiana against Diatraea saccharalis (Lepidoptera: Crambidae)
Provedor de dados: 52
Autores: Svedese,Virgínia Michelle; Lima,Elza Áurea de Luna Alves; Porto,Ana Lúcia Figueiredo.
The aim of this work was to evaluate the effect of temperature on the pathogenicity of Beauveria bassiana against Diatraea saccharalis and evaluate the horizontal transmission capacity among the sugarcane borer larvae. The strains were most pathogenic at 26ºC, followed by 32ºC than at 20ºC, with respective mortalities of 100, 50 and 30.3%. The lethal time was also affected by the temperature, with the shortest LT 50 observed at 26ºC (2.2 days) and the longest at 20ºC (16.2 days). The URM2915 strain appeared most effective in all the experiments as having potential for deployment in biological control programs for the sugarcane borer. Results in horizontal transmission of B. bassiana showed that this procedure could also be adopted as control strategy for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane borer; Lepidoptera; Entomopathogenic fungus; Biological control; Autodissemination.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300009

Biological control of phytopathogenic fungi by endophytic actinomycetes isolated from maize (Zea mays L.)
Provedor de dados: 52
Autores: Costa,Francisco Gheler; Zucchi,Tiago Domingues; Melo,Itamar Soares de.
This work aimed a survey on the biodiversity of maize endophytic actinomycete, and an evaluation of their potential to control the phytopathogenic fungi. From several regions of São Paulo state, 40 strains were isolated from the healthy maize plants. The identification of these strains, based on morphological properties and fatty acid methyl ester (FAME) profile showed that most of them belonged to the Streptomyces genus. These isolates were first screened for the growth inhibition of phytopathogenic fungi and results showed that all the isolate were able to inhibit the development of at least one tested pathogen. Two selected isolates were then evaluated for the control of P. aphanidermatum in cucumber (Cucumis sativa L.) under greenhouse conditions....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytes; Actinomycete; Streptomyces; Pythium aphanidermatum; Dumping-off.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600009

Quality Control of Biotechnological Inputs DetectingMycoplasma
Provedor de dados: 52
Autores: Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya; Garcia,Gabriel Henrique Oliveira; Timenetsky,Jorge.
The aim of this work was to study the Polymerase Chain Reaction (PCR) as a tool of quality control of bovine sera and cellular cultures used in the biotechnological industry. A total of 46 samples of bovine sera derived from two slaughterhouses and 33 samples of BHK21 cells derived from two biotechnological industries were evaluated using the primers GPO-3 (sense) and MGSO (antisense). The PCR technique sensibility analysis showed that 280 bp were amplified for the quantities of 50 ng to 0.006 ng of Micoplasma DNA. The primers specificity was confirmed in the test using Staphylococcus aureus, Escherichia coli, Bacillus subtilisand Candida albicans; except by the positive control, none of the samples showed amplification. The presence of Mycoplasma in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Bovine serum; BHK21 cells; Mycoplasma; Cellular culture.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200239

An Innovative Vessel for Research Studies at Amazon as a Potential Support for Sustainable Development and Conservation of the Region
Provedor de dados: 52
Autores: Meliande,Luís Otávio Vieira; Soares Filho,Afrânio de Amorim Francisco; Fonseca,Marcus Vinícius de Araújo; Miranda,Fernando Pellon de.
This study proposes an innovative and demonstrably necessary vessel intended for the research and logistic support of scientific or non-scientific activities in the Amazon, designed in accordance with the environmental and geographical requirements and characteristics of the region (possibly applicable to other similar hydrographic basins). The study also demonstrates the existence of a demand (actual and potential) for the infrastructure proposed. This infrastructure could subsidize the efforts devoted to the expansion of knowledge about the region, since many areas of science, institutions and business segments could benefit from its implementation. The access to knowledge could attract private investments with the perspective of developing business...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Research vessel; Research boat; Amazon; Sustainable development; Conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600979

Induction of ovulation in quarter horse mares through the use of deslorelin acetate and human chorionic gonadotrophin (hCG)
Provedor de dados: 52
Autores: Figueiredo,Tatiana; Paiva,Roman; Kozicki,Luiz Ernandes; Kaercher,Fernanda; Weiss,Romildo Romualdo; Santos,Ivo Walter dos; Muradas,Priscilla Ricabone.
The aim this study was to compare two protocols of induction for ovulation by desloreline acetate and hCG in Quarter Horse mares. The choice of the animals was based on the observations by the estrus, by rectal palpation of the ovaries and by ultrassonography of the follicular dynamics. After estrus detection and follicle control, the measurement of the follicles and the classification of uterus were carried out. The animals that had dominant follicle (diameter more than 35 mm) and swollen uterus were used. In these conditions, the mares received hCG or desloreline acetate. Once ovulation occurred, the artificial insemination was carried. Two groups were performed: G1 (20 animals) received 1.5 mg desloreline acetate and G2 (20 animals) received 1700 IU of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovulation; Deslorelin acetate; Human chorionic gonadotrophin; Quarter horse mares.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300012

Effects of the burrowing crab Chasmagnathus granulata (Dana) on meiofauna of estuarine intertidal habitats of Patos Lagoon, Southern Brazil
Provedor de dados: 52
Autores: Rosa,Leonardo Cruz; Bemvenuti,Carlos Emilio.
This study aimed to evaluate the effects of the burrowing crab Chasmagnathus granulata on meiofauna at three intertidal habitats across a tidal exposure gradient (i.e., an emerged salt marsh, an emerged mudflat and a submerged mudflat) in an estuarine embayment of Patos Lagoon, Southern Brazil. Meiofauna community was dominated by nematodes and ostracods, following by copepods and turbellarians. Densities of all studied organisms varied significantly among habitats. Highest values were observed in submerged mudflat while lower in salt marsh. Nematodes were unaffected by crab in either habitat, whereas ostracod, copepod and turbellarian densities were significantly lower in disturbed than control areas in both mudflat habitats. Any meiofaunal group was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Burrowing crab; Chasmagnathus granulata; Meiofauna; Disturbance; Natural experiment; Southern Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200014

Floral visitors and reproductive strategies in five melittophilous species of Bignoniaceae in Southeastern Brazil
Provedor de dados: 52
Autores: Yanagizawa,Yuriko A. N. Pinto; Maimoni-Rodella,Rita de Cassia Sindrônia.
In order to evaluate the pollination strategies of Bignoniaceae, the floral biology and the floral visitors in five species, three cerrado shrubs (Arrabidaea brachypoda (DC.) Bor., Jacaranda decurrens Cham., and Jacaranda oxyphylla Cham.), and two lianas from the border of a semideciduous seasonal forest (Arrabidaea samydoides (Cham.) Sandw., and Arrabidaea triplinervia H. Baill.) were studied in Botucatu, São Paulo State, Southeastern Brazil. The flowering periods were partially overlapping, especially between species in the same habitat. All the five species were functionally allogamous, melittophilous, nototribic and mainly pollinated by long tongued large bees. Some medium-sized and small pollen-foraging bees were occasional legitimate visitors,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arrabidaea; Jacaranda; Melittophilly; Pollination; Breeding system.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700015

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro
Provedor de dados: 52
Autores: Dibax,Roberson; Quisen,Regina Caetano; Bona,Cleusa; Quoirin,Marguerite.
The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 µM NAA and 4.44 µM BAP. The best results for bud regeneration were obtained on MS and WPM media (57.5 and 55% of calluses formed buds, respectively). Shoot elongation and rooting (80%) were obtained on MS/2 medium (with half-strength salt concentration) with 0.2% activated charcoal. Acclimatization was performed in the growth chamber for 48 h and then the plants were transferred to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adventitious buds; Callogenesis; Organogenesis; Tissue culture; Anatomical study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200009

Stream-dwelling fish communities from an Atlantic rain forest drainage
Provedor de dados: 52
Autores: Mazzoni,Rosana; Fenerich-Verani,Nelsy; Caramaschi,Érica Pellegrini; Iglesias-Rios,Ricardo.
Twenty-two species were registered in the Ubatiba system with a predominance of Characiformes and Siluriformes followed by Cyprinodontiformes, Perciformes and Synbranchiformes. Among these species four were occasional with one or two sampled individuals and restricted to one or two of the nine studied sites; the other 18 species were constant with high densities in at least one site. Most of the sampled sites presented between 11 and 18 species, all largely distributed; except for the uppermost site, located right over a waterfall 4m high, which showed at least four species and for the confluence site that presented 18 species. Canonical Correspondence Analysis indicated that environmental variables explained a great part of the density and distribution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal stream; Distribution; Community structure; Fish.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000300010

Lactococcus bacteriophages isolated from whey and their effects on commercial lactic starters
Provedor de dados: 52
Autores: Oriani,Maria Raquel de Godoy; Yokoya,Fumio.
The incidence of phages of lactic acid bacteria in milk industry and their effects on acidification ability of commercial lactic acid starters were studied. Cheese whey samples (33 samples) were collected from 17 factories. A total of 16 bacteriophages were isolated (12 specific for Lactococcus lactis, 3 for L. diacetylactis and one capable of lysing both species). The results showed that 10% reduction in acidification tests was not good indication of phage in the sample. The majority of samples showed reduction higher than 10%, although only 65% were phage positive. The isolated phages were quite stable and showed no reduction in infectivity even after 20 daily replications. A pool of bacteriophages was prepared from isolates and inoculated in 12...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriophage; Lactococcus; Cheese making; Milk; Processing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400009

Development and optimization of extractives from Astronium urundeuva (allemão) Engl. by factorial design
Provedor de dados: 52
Autores: Cunha,Fabíola Pessoa da; Costa,Luana Janine Lopes da; Fernandes,Ana Josane Dantas; Souza,Tatiane Pereira de; Soares,Luiz Alberto Lira.
The purpose of this study was to evaluate the influences from variables of preparation on dry residue and total tannins from macerates of Astronium urundeuva barks. Thus, a 3 ² factorial design was used to study the importance of plant proportion (10, 15 and 20 %, w/v) and the ethanol concentration (40, 60 and 80 %, v/v) on the selected response. Mathematical models were fitted according to experimental data. The validated equations were used to generate response surfaces. The analysis of the surfaces showed that the optimum conditions to obtain extractive preparations with high extractive efficiency for tannins were: 10 % (w/v) of plant with ethanol 40 % (v/v) as solvent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astronium urundeuva; Extraction; Optimization; Factorial Design.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300017

Denitrification coupled with methane anoxic oxidation and microbial community involved identification
Provedor de dados: 52
Autores: Cuba,Renata Medici Frayne; Duarte,Iolanda Cristina; Saavedra,Nora Katia; Varesche,Maria Bernadete Amâncio; Foresti,Eugenio.
In this work, the biological denitrification associated with anoxic oxidation of methane and the microbial diversity involved were studied. Kinetic tests for nitrate (NO3-) and nitrite (NO2-) removal and methane uptake were carried out in 100 mL batch reactors incubated in a shaker (40 rpm) at 30 ºC. Denitrificant/methanotrophic biomass was taken from a laboratory scale reactor fed with synthetic nitrified substrates (40 mgN L-1 of NO3- and subsequently NO2-) and methane as carbon source. Results obtained from nitrate removal followed a first order reaction, presenting a kinetic apparent constant (kNO3)) of 0.0577±0.0057d-1. Two notable points of the denitrification rate (0.12gNO3--N g-1 AVS d-1 and 0.07gNO3--N g-1 AVS d-1) were observed in the beginning...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Denitrification; Methylomonas sp; Methane oxidation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100022

Study of banana (Musa aaa Cavendish cv Nanica) trigger ripening for small scale process
Provedor de dados: 52
Autores: Larotonda,Fábio Donato Soares; Genena,Aziza Kamal; Dantela,Daniela; Soares,Hugo Moreira; Laurindo,João Borges; Moreira,Regina Fátima Peralta Muniz; Ferreira,Sandra Regina Salvador.
The present work focuse on the impact of O2, CO2 and ethylene concentrations on ripening rate control of bananas as a contribution for the development of domestic equipments that could allow the user to drive the fruit shelf live. It represented the adjustment of metabolic activity rates in order to manage the maturity process. Ripening variables such as ethylene and CO2 concentrations and temperature were adjusted to accelerate or slow down the process, while the maturity degree was monitored through the physical and chemical parameters and sensorial analysis. Therefore, the objective of this work was to evaluate the influence of these parameters to manage the banana ripening. The optimum temperature was at 25 ÂºC of storage. The presence of oxygen, CO2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Banana; Ripening; Trigger; Ethylene; CO2.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500021

Effectiveness of Origanum vulgare L. and Origanum majorana L. essential oils in inhibiting the growth of bacterial strains isolated from the patients with conjunctivitis
Provedor de dados: 52
Autores: Oliveira,Jana Luíza Toscano Mendes de; Diniz,Margareth de Fátima Melo; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Trajano,Vinícius Nogueira; Santos,Bernadete Helena Cavalcante.
This study aimed to evaluate the antibacterial activity of Origanum vulgare L. and O. majorana L. essential oils on Staphylococcus aureus, S. coagulase negative, Enterobacter spp., Proteus spp., Acinetobacter spp., Klebsiella spp. isolated from the patients with conjunctivitis. The results showed a prominent inhibitory effect of both the essential oils on all the bacterial strains, noted by the large bacterial growth inhibition zones (15-32mm). The Minimum Inhibitory Concentrations (MIC) values were between 5-20µL/mL and 2.5-10 µL/mL for O. vulgare and O. majorana essential oil, respectively. The MIC were able to cause significant (P&lt;0.05) inhibitory effect on the cell viability of Klebsiella spp., Proteus spp. and S. aureus providing a total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Origanum vulgare L.; Origanum marjorana L.; Essential oil; Antibacterial activity; Conjunctivitis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100006

Measurement of thermophysical properties of ceramics by the flash method
Provedor de dados: 52
Autores: Pinto,Cláudio S. C.; Massard,Henrique; Couto,Paulo; Orlande,Helcio R. B.; Cotta,Renato M.; Ambrosio,Maria C. R.
The flash method, proposed by Parker, Butler, Jenkins and Abbott from the U.S. Navy Radiological Defense Laboratory in 1961, is the most popular method for measuring the thermal diffusivity of solids. In this method, the front surface of a small sample is subjected to a very short burst of radiant thermal energy. The resulting temperature rise on the opposite surface of the sample is measured and the thermal diffusivity is computed from the temperature rise versus time data. Also, the specific heat can be computed from the measured data, thus allowing for the calculation of the thermal conductivity. Several theoretical models are available for the flash method, which include adiabatic boundary conditions, heat losses, surface coating effects, among other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flash Method; Thermophysical Property; Ceramic block.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200006

Absence of mutagenic effect of Mikania glomerata hydroalcoholic extract on adult wistar rats in vivo
Provedor de dados: 52
Autores: Sá,Rita de Cássia da Silveira e; Leite,Magda Narciso; Peters,Vera Maria; Guerra,Martha de Oliveira; Almeida,Reinaldo Nóbrega de.
This work makes an assessment of the dominant lethality of Mikania glomerata in male Wistar rats. Adult male received 1 mL of M. glomerata hydroalcoholic extract at a dose level of 3.3 g/kg body weight for 52 days and were mated with untreated females for seven weeks (group 1) or one week prior to the beginning of treatment and on the week following the end of treatment (group 2). The parameters analyzed were: number of implanted embryos, resorptions and corpora lutea; mating, gestation, preimplantation loss, implantation and resorption indexes (group 1); number of offspring and weaning animals (group 2). The administration of M. glomerata did not show any impairment of fertility and no significant difference in the parameters analyzed, suggesting an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mikania glomerata; Mutagenicity; Wistar rat.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500009

Elementary chemical analysis in leaves infected by fumagina by X-Ray fluorescence technique
Provedor de dados: 52
Autores: Blonski,Maria Sélia; Appoloni,Carlos Roberto; Parreira,Paulo Sérgio; Aragão,Pedro Henrique Arruda; Nascimento Filho,Virgilio Franco.
Energy Dispersion X-Ray Fluorescence Technique (EDXRF) was employed to study the effects of the fumagina disease on the elementary chemical composition of the leaves. The experimental set up consisted of a Mo X-ray tube (Ksub&lt;FONT FACE=Symbol&gt;µ&lt;/FONT&gt; = 17.44 keV) with Zr filter and a Si (Li) detector. The measurements were performed with the infected and healthy leaves of citric plants. The elements Ti, Mn, Fe, Cu and Zn were quantified, with an average DL of 69, 12, 8, 4 and 4 µg.g-1 respectively. The obtained concentration for Fe varied from 44 to 192 µg.g-1 in healthy leaves and from 363 to 704 µg.g-1 in infected leaves with fumagina .
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fumagina; Leaves; Fluorescence of X-rays.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500013

Seasonal Variation of Plant Mineral Nutrition in Fruit Trees
Provedor de dados: 52
Autores: Cruz,André Freire; Almeida,Glicia Maria de; Wadt,Paulo Guilherme Salvador; Pires,Marcio de Carvalho; Ramos,Maria Lucrecia Gerosa.
Abstract This research evaluated the monthly variation of plant mineral nutrition in six species of fruit trees over a year. Leaf samples were taken from the fruit trees and nutritional status (N, P, K, Ca, Mg, Fe, Zn, Cu, Mn, and Mo) was determined in the leaves in a month basis from April until November for apple, persimmon and peach. Mandarin mineral nutrition was monitored for one year, and grape and fig from May to November. Using this data, the Diagnosis Recommendation Integrated System (DRIS) was also calculated to evaluate the nutrient balance in the plants. The concentration of N and P had seasonal differences, especially in apple and peach, which reached the peak during the summer. Apple, fig, and grape trees had large ranging on their mineral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis Recommendation Integrated System; Fruit Crops; General Plant Nutrition; Nutrient balance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100212

Inhibition of ascitic ehrlich tumor cell growth by intraperitoneal injection of Pfaffia paniculata (Brazilian ginseng) butanolic residue
Provedor de dados: 52
Autores: Vieira,Juliana; Matsuzaki,Patricia; Nagamine,Márcia Kazumi; Haraguchi,Mitsue; Akisue,Gokithi; Gorniak,Silvana Lima; Dagli,Maria Lúcia Zaidan.
This study aimed to investigate the effects of the administration of butanolic residue (BR) of Pfaffia paniculata by intraperitoneal route to Ehrlich ascitis tumor bearing mice. Initially, a toxicity study of P. paniculata BR was performed in which doses of 12.5; 25 and 50mg/Kg were administered by intraperitoneal injection for seven days to Swiss mice. The treatment did not show toxicity. Then, Swiss male mice received, by intraperitoneal injection, once a day, 12.5; 25 or 50mg/Kg of P. paniculata BR for seven days. This protocol started in the same day of tumor inoculation with 5X10(6) cells i.p. The treatment with butanolic residue of P.paniculata i.p caused a significant increase in the ascitic volume; however, a significant decrease in tumor cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pfaffia paniculata; Ehrlich; Intraperitoneal treatment.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300014

Biological traits and Life table parameters A and B biotype of Bemisia tabaci (Genn.) on cotton and rapeseed
Provedor de dados: 52
Autores: Samih,Mohammad Amin; Zarabi,Mehdi; Yazdani,Maryam; Rouhani,Mohammad.
The aim of this work was to construct life table of Bemisia tabaci (Gen.) A and B (silverleaf whitefly B. argentifolii Bellows and Perring) biotype (Hem.: Aleyrodidae) on two host plants; cotton, (Gossypium hirsutum L.) and rapeseed, (Brassica napus L.). Experiments were conducted in a growth chamber under 24 ± 2ºC, 55±3% RH and 16:8 (L:D) h photoperiod on caged plants of cotton G. hirsutum L. (Varamin 76 variety) and rapeseed B. napus L. (global variety. The intrinsic rate of natural increase (r m), net reproductive rate (R0) and mean generation time (T) for B. tabaci A biotype was 0.1010 females per female per day, 18.4075 females per female and 30.079 day (d) on cotton; 0.1286, 30.6760 and 26.77 d on rapeseed; and for B biotype (B. argentifolii) those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bemisia argentifolii; Bemisia tabaci; Cotton; Life-table; Rapeseed.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300001

Productive and Reproductive Perfomance of Dairy Cows During Peripartum
Provedor de dados: 52
Autores: Pinto Neto,Adalgiza; Menegoto,Juliano; Skonieski,Fernando; Mota,Marcelo Falci; Martinez,Antônio Campanha; Merlini,Luiz Sérgio; Berber,Rodolfo Cassimiro de Araujo.
ABSTRACT Dairy activity is of great importance for the national economy. It is also an important source of income for families in the south east region of Paraná that work in this area. There has been a notable expansion in the activities as well as in the quantity of production and the quality of the products. With this has come problems in the reproductive efficiency of the dairy cattle principally in relation to those with the greatest production. The objective of this study was to evaluate the productive profile and the reproductive efficiency of dairy cows. The evaluation covered 30 days before calving and 15, 30, 45, 60 and 90 days after the calving. The study covered cows from two properties in the Municipality of Realza-PR in Brazil. In order to do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cows; BCS; Uterine involution; Postpartum ovulation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100317

Production of extracellular protease by a Brazilian strain of Beauveria bassiana reactivated on coffee berry borer, Hypothenemus hampei
Provedor de dados: 52
Autores: Ito,Eliana Tiemi; Varéa-Pereira,Geni; Miyagui,Dalva Tomoe; Pinotti,Maria Helena Pimenta; Neves,Pedro Manoel Oliveira Janeiro.
Studies were carried out on extracellular protease production by Beauveria bassiana CG432 in liquid medium containing glucose and yeast extract. B. Bassiana presented active growth after lag period of 24 h., produced 80% of the total of the extracellular protease activity in 48 h which was maximum on the 5th culture day. The extracellular protease presented optimum activity at 60ºC, was stable up to 1M Cl-, maintained the stability during 15 day at 4ºC and -18ºC, but was not stable if frozen repeatedly.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease activity; Beauveria bassiana; Enzyme stability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200006

Development of a certified reference material: ethanol in water - a practical case
Provedor de dados: 52
Autores: Souza,Vanderléa de; Caixeiro,Janaína Marques R.; Silva,Rafael A. L. da; Pedro,Leandro R.; Borges,Renata M. H.; Couto,Paulo R. G.
The present work describes the development of a certified reference material (CRM) composed of solutions of ethanol in water in five different concentrations, and all stages necessary for the development of an CRM, according to the guidelines of ISO GUIDES 30, 31, 32, 33, 34 and 35. The development of this CRM has been determined by Inmetro Rule n. 006 of 2002, which requires that all breath alcohol analyzers being used in Brazil shall have their respective models approved and that the subsequent verifications shall be performed based on tests with solutions of ethanol in water. As a result, the development of this CRM significantly contributes to the reliability of measurements performed with breath alcohol analyzers, with stated measurement uncertainty....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Certified reference material; Reliability of measurements; Ethanol.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200017

Epibenthic colonization on an artificial reef in a stressed environment off the north coast of the Rio de Janeiro State, Brazil
Provedor de dados: 52
Autores: Krohling,Werther; Zalmon,Ilana Rosental.
The influence of sedimentation and turbidity caused by the pluviometric precipitation and by the Paraiba do Sul River during the summer was investigated on an artificial reef along the north coast of Rio de Janeiro, Brazil. Four recruitment and four cumulative plates were removed monthly (January 2002 to February 2003) from the external surface of the concrete reef modules. Empty space was not a limiting factor the recruitment. Turnover rate and structural difference index were higher in the first months and reflected low change in the species dominance during almost the entire study period. The high correlation between the precipitation and the empty space suggested that rain influence the recruitment and the succession. The recruitment was possible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recruitment; Succession; Benthic community structure; Natural disturbance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100025

An Empirical Evaluation of the Local Texture Description Framework-Based Modified Local Directional Number Pattern with Various Classifiers for Face Recognition
Provedor de dados: 52
Autores: Rose,R. Reena; Meena,K.; Suruliandi,A.
ABSTRACT Texture is one of the chief characteristics of an image. In recent years, local texture descriptors have garnered attention among researchers in describing effective texture patterns to demarcate facial images. A feature descriptor titled Local Texture Description Framework-based Modified Local Directional Number pattern (LTDF_MLDN), capable of encoding texture patterns with pixels that lie at dissimilar regions, has been proposed recently to describe effective features for face images. However, the role of the descriptor can differ with different classifiers and distance metrics for diverse issues in face recognition. Hence, in this paper, an extensive evaluation of the LTDF_MLDN is carried out with an Extreme Learning Machine (ELM), a Support...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Face Recognition; Texture Descriptors; Local Texture Description Framework; Local Texture Description Framework-Based Modified Local Directional Number Pattern; Classifiers; Distance Metrics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300405

Feeding habits of Stellifer rastrifer (Perciformes, Sciaenidae) at Guaratuba mangrove, Parana, Brazil
Provedor de dados: 52
Autores: Chaves,Paulo de Tarso C.; Vendel,Ana Lúcia.
Feeding habits of Stellifer rastrifer (Perciformes, Sciaenidae) at Guaratuba mangrove, Parana, Brazil, were studied from February 1996 to February 1997. It was observed that its diet was based on invertebrates, mainly Decapoda non-Brachyura and Polychaeta. In a smaller proportion there were plants, Copepoda, Gammaridea and Mollusca. The level of contribution of each food item changed according to the season and the individual size. Such plasticity in feeding behaviour was similar to that described to some fish populations from other estuaries, and could be an indicator of the high level of instability presented by this kind of ecosystem.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding habit; Stellifer rastrifer; Sciaenidae; Mangrove; Guaratuba; Parana.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400006

Ontogeny and Structure of the Pericarp of Schinus terebinthifolius Raddi (Anacardiaceae)
Provedor de dados: 52
Autores: Carmello-Guerreiro,Sandra Maria; Paoli,Adelita A. Sartori.
The fruit of Schinus terebinthifolius Raddi is a globose red drupe with friable exocarp when ripe and composed of two lignified layers: the epidermis and hypodermis. The mesocarp is parenchymatous with large secretory ducts associated with vascular bundles. In the mesocarp two regions are observed: an outer region composed of only parenchymatous cells and an inner region, bounded by one or more layers of druse-like crystals of calcium oxalate, composed of parenchymatous cells, secretory ducts and vascular bundles. The mesocarp detaches itself from the exocarp due to degeneration of the cellular layers in contact with the hypodermis. The lignified endocarp is composed of four layers: the outermost layer of polyhedral cells with prismatic crystals of calcium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anacardiaceae; Schinus terebinthifolius; Pericarp; Anatomy; Pericarpo; Anatomia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100012

Cloning of Ammopiptanthus mongolicus C-repeat-binding factor gene and its cold-induced tolerance in transgenic tobacco
Provedor de dados: 52
Autores: Gu,Lijiang; Zhang,Han; Guo,Huiming; Cheng,Hongmei.
C-repeat-binding factors (CBFs) are a type of important regulon in stress-related signal transduction pathways that control plant tolerance of abiotic stress. Ammopiptanthus mongolicus is the only evergreen broadleaf shrub in the northwest desert of China. The species shows strong resistance to environmental stress, especially to cold stress. An A. mongolicus CBF1 gene (AmCBF1) was cloned and transformed into tobacco. Expression of AmCBF1 could be detected in A. mongolicus shortly after exposure to low temperature of 4°C. Analysis on ratio of electrolytic leakage, soluble sugar content, free proline content, malondialdehyde (MDA) content and peroxidase (POD) activity before and after cold treatment (4°C) for 24 h indicated AmCBF1 conferred higher cold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammopiptanthus mongolicus; C-repeat-binding factor; Gene transformation; Physiological index detection.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600002

LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
Provedor de dados: 52
Autores: Onofre,Sideney Becker; Riveros Gonzalez,Raul; Messias,Cláudio Luiz; Azevedo,João Lúcio; Barros,Neiva Monteiro de.
The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticarsia gemmatalis; Nomuraea rileyi; Integrated control; Secondary metabolite; Toxins.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004

Biosorption of anionic textile dyes from aqueous solution by yeast slurry from brewery
Provedor de dados: 52
Autores: Castro,Ketinny Camargo de; Cossolin,Aline Silva; Reis,Hélen Cristina Oliveira dos; Morais,Eduardo Beraldo de.
ABSTRACT This study investigated the biosorption of the anionic textile dyes: Reactive Red 239 (RR239), Reactive Black B (RBB) and Direct Blue 85 (DB85) according to pH, biomass dosage, contact time and dye concentration onto waste beer yeast slurry. The kinetics and isotherm of the removal of dyes were also studied. The equilibrium of biosorption reaction was reached after 30 min for the reactive dyes and after 60 min for the direct dye. Optimum decolorization was observed at pH 2 and 0.63 g/L of biomass dosage. The kinetic data of the three dyes were better described by the pseudo second-order model. The adsorption process followed the Langmuir isotherm model and the biosorption capacity being estimated to be 152.9, 162.7 and 139.2 mg/g for RR239, RBB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioadsorptive decolorization; Waste beer yeast slurry; Biosorption kinetics; Biosorption isotherms.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100602

Aquatic macrophytes of Itaipu Reservoir, Brazil: survey of species and ecological considerations
Provedor de dados: 52
Autores: Thomaz,Sidinei Magela; Bini,Luis Maurício; Souza,Maria Conceição de; Kita,Kazue Kawakita; Camargo,Antonio Fernando Monteiro.
In a survey of the aquatic macrophytes of the Itaipu Reservoir, we identified 62 taxa in 25 families and 42 genera. The highest number taxa was observed for the emergent macrophytes (40 taxa). Reduced fluctuation in water level, increased light penetration, and sediment enrichment by nutrients and organic matter following the formation of the reservoir favored the appearance of a species-rich community of submerged macrophytes (23% of the taxa identified). The aquatic macrophytes were found mainly near the mouths of the main tributaries of the reservoir, in shallow area of depth less than 2 meters. In addition to the shallow depth, the greater nutrient input from the tributaries and relative protection from wind explained this distribution. Among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuisance species; Aquatic macrophytes; Itaipu reservoir; Egeria najas; Egeria densa.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100003

Proximate composition, minerals and fatty acid composition of Juglans Regia L. genotypes and cultivars grown in Turkey
Provedor de dados: 52
Autores: Yerlikaya,Cemile; Yucel,Sevil; Erturk,Ümran; Korukluoğlu,Mihriban.
The proximate composition of eleven walnut (Juglans regia L.) genotypes (28 ŞK 010, 28 ŞK 055, 28 ŞK 041, 28 ŞK 601, 28 ŞK 925, 28 ŞK 028, 28 ŞK 118, 28 ŞK 350, 28 ŞK 930, 28 ŞK 850, 28 ŞK 036) and three walnut cultivars (Şebin, Bilecik, Kaman 1) produced in Turkey were determined. The oil content of the samples ranged from 61.32 to 69.35%, corresponding to an energy value of approximately 710 kcal per 100 g of kernel. The protein content ranged from 10.58 to 18.19%, and the carbohydrate composition was between 9.05 and 18.92%. The ash content ranged from 1.53 to 1.99%, and the moisture content of the kernels was between 1.91 and 4.48% the oleic acid content of the oils ranged from 17.90 to 33.35% of the total fatty acids. The linoleic acid content ranged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juglans regia L; Walnut; Proximate composition; Fatty acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500006

Influence of the frying recipients in the quality of soy and refined nhandiroba vegetable oils
Provedor de dados: 52
Autores: Freitas,Lindeberg Rocha; Ventura,Ana Paula Moraes; Queiroga,Rita de Cássia Ramos do Egypto; Paulo,Marçal de Queiroz.
The main objective of this work was to quantify the migration of iron and aluminium ions and to evaluate the influence of recipients made of iron, stainless steel, aluminium and glass on the oxidative stability during frying with soy (Glycine max) and refined nhandiroba oils (Fevillea trilobata). Soy oil during the frying process in the four distinct recipients presented increased Free Fatty Acids index (FFA) and Peroxide index (PI) values and decreased Iodine index (II) values. Refined nhandiroba oil presented decreased FFA rates in all the recipients investigated. The analysis of results showed that soy oil presented relevant oxidative alterations when compared to the heating time of 72 h and the types of frying recipients used, and in iron and stainless...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetal oils; Frying; Oxidative stability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100025

Pulsed field gel electrophoresis reveals chromosome length and number differences in Brazilian strains of Metarhizium Anisopliae
Provedor de dados: 52
Autores: Kava-Cordeiro,Vanessa; Queiroz,Marisa Vieira de; Pizzirani-Kleiner,Aline Aparecida; Azevedo,João Lúcio.
Electrophoretic karyotypes of eight wild-type strains of Metarhizium anisopliae var. anisopliae were obtained by pulsed-field gel electrophoresis. These strains were isolated from insects of six different Brazilian states. The chromosomal DNA molecules of three strains were separated into seven bands and of five strains into eight bands. Chromosome length polymorphisms were also observed. The size of the chromosomal DNA of all strains varied between 7.7 and 0.9 Mb using the Aspergillus nidulans chromosomes as size standards. The total genome size of these strains was estimated in at least 29.7 Mb. Some correlations between differences in karyotype and occurrence of parasexual cycle likewise the host specificity were discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium anisopliae; Pulsed-field gel electrophoresis; Chromosome polymorphisms; Parasexual cycle; Entomopathogenic fungus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100001

Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents
Provedor de dados: 52
Autores: Rodrigues,Marcelo; Festucci-Buselli,Reginaldo Alves; Silva,Luzimar Campos; Otoni,Wagner Campos.
The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neem; HPLC; Glucose; Hydrolysed casein; Methyl jasmonate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001

Effect of plastic mulch on growth and yield of chilli (Capsicum annuum L.)
Provedor de dados: 52
Autores: Ashrafuzzaman,M; Halim,M. Abdul; Ismail,Mohd Razi; Shahidullah,S.M; Hossain,M. Alamgir.
In this work a field study was conducted to evaluate the effect of coloured plastic mulch on growth and yield of chilli from October 2005 to April 2006. The plastic mulches were transparent, blue, and black and bare soil was the control. Different mulches generated higher soil temperature and soil moisture under mulch over the control. Transparent and blue plastic mulches encouraged weed population which were suppressed under black plastic. Plant height, number of primary branches, stem base diameter, number of leaves and yield were better for the plants on plastic. At the mature green stage, fruits had the highest vitamin-C content on the black plastic. Mulching produced the fruits with the highest chlorophyll-a, chlorophyll-b and total chlorophyll...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum annum; Chilli; Growth; Plastic mulch; Yield; Yield attributes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200014

Erratum: Expression, Purification and Functional Assessment of Smallest Isoform of Human Interleukin-24 in Escherichia coli
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100910

Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages
Provedor de dados: 52
Autores: Demir,Ibrahim; Mavi,Kazım.
Germination percentage, seedling fresh weight and sensitivity index of the pepper (Capsicum annuum L. cv. Sera Demre) seed lots harvested 50, 60 and 70 days after the anthesis (daa) in 2001 and 2002 were determined under the salt (NaCl) and osmotic (PEG) stresses at the same water potentials of -0.3, -0.6 and -0.9 MPa. The objective was to study whether the germination and seedling growth were inhibited by the salt toxicity or osmotic effect during the development. The higher the salt and osmotic stress concentrations the lower was the germination percentage and seedling fresh weight. The seed germination was higher and sensitivity index were lower in NaCl than in PEG at the same water potential and harvest in both the years. They were able to germinate at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum annuum; Salinity; Water stress; Germination.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500004

Characterization and evaluation of the impact of feed management on the effluents of Nile tilapia (Oreochromis niloticus) culture
Provedor de dados: 52
Autores: Baccarin,Ana Eliza; Camargo,Antonio Fernando Monteiro.
The objective of this study was to evaluate the effect of different feed management on the quality of effluent water generated in Nile tilapia (Oreochromis niloticus) culture ponds. Feed was supplied as follows: natural food, and pelleted, extruded or minced ration. The study was conducted during 19 weeks in 12 continuous water exchange ponds of 300 m² each, with a population of male Nile tilapia juveniles, in a density equal to 1.7 fish m-2. Feeds contained 30% crude protein, 3,000 kcal digestible energy, and were supplied twice a day. Natural food consisted of chicken manure. Temperature, dissolved oxygen, pH, total phosphorus, total nitrogen, chlorophyll a and suspended solids were monitored weekly in the supplying and effluent water. Water residence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish culture; Environmental impact; Feed management; Effluent.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100012

Crucial Residues Modulating Interface of hBcl-B - hBaxBH3 Heterodimer as Probed by Computational Methods
Provedor de dados: 52
Autores: Sivakumar,Dakshinamurthy; Sivaraman,Thirunavukkarasu.
ABSTRACT Cancerous cells develop resistance to cell death by over expression of anti-apoptotic proteins, which are specific to interact with pro-apoptotic and BH3-only proteins of Bcl-2 family. Delineating crucial residues mediating the heterodimer complexes (anti-apoptotic proteins - pro-apoptotic/BH3-only proteins) is indispensable to develop specific antagonists to anti-apoptotic proteins. In these backgrounds, we have herein reported crucial residues of hBaxBH3 and hBcl-B (an anti-apoptotic protein specifically interacts with human Bax but does not interact with human Bak) for hetero dimerization of the polypeptides and as well validated the structural determinants of the polypeptides through variety of virtual 'alanine mutants' and 'switch mutants' by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Anti-apoptotic proteins; BH3-only peptides; Dimer interface; Dockings; Virtual mutants.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100422

Molecular cloning of αRYR hotspot region 1 from broiler chicken
Provedor de dados: 52
Autores: Ziober,Iris Lamberti; Paião,Fernanda Gonzales; Marin,Silvana Regina Rockenbach; Marchi,Denis Fabricio; Binneck,Eliseu; Nepomuceno,Alexandre Lima; Coutinho,Luiz Lehmann; Shimokomaki,Massami.
Samples of Pectoralis major m. were collected, and an RT-PCR analysis of the a-Ryanodine receptor (a RYR) from chicken mRNA hotspot region spanning aminoacid residues 386 to 540, numbered according to the turkey sequence, revealed two classes of transcripts. The sequences of the first class were similar to turkey and human with 97% and 74% of identity, respectively, and included all transcripts with substitutions in the nucleotide sequence. The second class was characterized by the deletion of nucleotides, leading to a premature stop codon and coding for a truncated and nonfunctional protein. These results are to date the first report related to the sequencing of the chicken αRYR hotspot region 1, which will possibility serve as a guide for further studies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ΑRYR; PSE; Chicken.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700029

Redescrição de Cymodoce madrasensis (Srinivasan, 1959) combinação nova (Sphaeromatidae: Isopoda: Crustacea) de Madras, Índia
Provedor de dados: 52
Autores: Silva,Jayme de Loyola e.
O autor estudou quase todas as espécies da família Sphaeromatidae (Isopoda), da Coleção de Crustacea do USNM, e a espécie constante do catálogo 102151 USNM, na época, não possuia identificação razão pela qual o autor enquadrou no gênero Cymodoce. Hoje está classificada como Exosphaeroma madrasensis Srinivasan, 1959. Este gênero não condiz com as características da espécie motivo pelo qual o autor procedeu a transferência para o gênero Cymodoce Leach, 1814, e estabeleceu nova combinação Cymodoce madrasensis (Srinivasan, 1959).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isopoda; Sphaeromatidae; Cymodoce madrasensis; Taxonomy; India.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300015

Environmental quality evaluation of lakes in the Rio Grande do Sul coastal plain
Provedor de dados: 52
Autores: Pedrozo,Catarina da Silva; Rocha,Odete.
The purpose of the present work was to evaluate the environmental quality of Marcelino, Pinguela, Peixoto, Palmital, Malvas and Do Passo lakes, interconnected by canals and located in the district of Osório, RS. In this context, 29 physical and chemical variables were analyzed with sampling carried out in January, May, July and October 1997 to verify the environmental quality of the system and the existence of a gradient of quality. Canonical Analysis was applied to the data of all environmental variables measured, and showed that the lakes had different characteristics as a consequence of the organic pollution received. Marcelino lake received continuous input of organic matter from the sewage treatment works in the town of Osório, whereas Peixoto,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Castal lakes; Water quality; Environmental variables; Limnological characteristics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400013

Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
Provedor de dados: 52
Autores: Núñez,Eutimio Gustavo Fernández; Jorge,Soraia Attie Calil; Astray,Renato Mancini; Rezende,Alexandre Gonçalves de; Costa,Bruno Labate Vale da; Monteiro,Daniella Cristina Ventini; Pereira,Carlos Augusto; Tonso,Aldo.
The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BHK-21; Large-scale bioprocesses; Mammalian cells; Recombinant proteins; SFV; Transient expression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018

Erratum: Enterococcus spp. Resistant to Multiple Antimicrobial Drugs and Determination of Fecal Contamination Levels in Mangrove Oysters (Crassostrea rhizophorae)
Provedor de dados: 52
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100903

Trophic state and microorganisms community of major sub-basins of the middle Rio Doce basin, southeast Brazil
Provedor de dados: 52
Autores: Petrucio,Mauricio Mello; Medeiros,Adriana Oliveira; Rosa,Carlos Augusto; Barbosa,Francisco Antônio Rodrigues.
Total phosphorus concentration was used to define the trophic state of the main sub-basins of the middle Rio Doce, in Minas Gerais State (southeast Brazil) and physical, chemical, and microbiological variables of water were analyzed during 2000 to 2001. The study evaluated changes in water quality caused by seasonality and human activities. Water temperature, conductivity, pH, total alkalinity, dissolved oxygen, and concentrations of NH4-N, NO2-N, NO3-N, total-N, PO4-P, total-P, DOC, and chlorophyll-a were measured in seven rivers stretches (sampling stations). Total yeasts, faecal and total coliforms, and heterotrophic bacteria were also determined. The studied areas were considered to be under oligotrophic to eutrophic conditions. The variables that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microorganisms; Phosphorus; Rivers; Trophic state.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500015

Genotypic diversity and virulence factors of Streptococcus mutans in caries-free and caries-active individuals
Provedor de dados: 52
Autores: Braga,Miula Portelinha; Piovesan,Augusta; Valarini,Natália; Maciel,Sandra Mara; Andrade,Flaviana Bombarda de; Poli-Frederico,Regina Célia.
The objective of this study was to analyze the genotypic diversity, frequency of serotypes and the detection of mutacins from Streptococcus mutans isolates in caries-free and caries-active individuals.A total of 260 S. mutans isolated from 28 individuals with and without dental caries were subjected to AP-PCR and PCR screening of glucosyltransferase B, mutacin and serotype genes, which showed the presence of. 70 different genotypes. There was no statistically significant association between the presence of genes for serotypes and mutacins with dental caries. However, there was a statistically significant and a strong association between the higher genotypic diversity in the subjects with caries (r = 0.72, p = 0.001). There was an increase in the number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental caries; Genotypic diversity; Mutacin; Serotype; Streptococcus mutans.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200009

In Vitro study of Vellozia pusilla pohl (Velloziaceae), a Brazilian plant species: antitumoral activity and labeling of blood elements
Provedor de dados: 52
Autores: Dantas,Ana Letícia Almeida; Valente,Lígia Maria Marino; Morais,Lilia Aparecida Salgado de; Feliciano,Glaucio; Bernardo-Filho,Mário.
Vellozia pusilla Pohl is a species of the botanic family Velloziaceae that occurs in the subtropical regions of South America and, although it lives under conditions of high solar irradiation and low water availability, shows great longevity. The methanol extract of roots, stem and leaf sheaths of this species showed an antitumoral activity through the inhibition of the enzyme Topoisomerase I when analyzed by an in vitro bioassay employing DNA repair or recombination deficient mutants of the yeast Saccharomyces cerevisiae. In the evaluation of the effect of Vellozia pusilla methanol extract on the labeling of RBC, blood of mice was treated with 99mTc tracer solutions. The percentage of radioactivity (%ATI) bound to plasma (P) and blood cells (BC) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug interaction; Antitumoral activity; Technetium-99m; Blood elements; Velloziaceae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700008

Effects of light on the growth and photosynthesis of Egeria najas planchon
Provedor de dados: 52
Autores: Tavechio,Washington Luiz Gomes; Thomaz,Sidinei Magela.
Photosynthesis and growth of Egeria najas (Hydrocharitaceae) from a subtropical reservoir (Itaipu Reservoir, Brazil-Paraguay) were measured in response to low light intensities (0-124 µM m-2 s-1 PAR) in the laboratory. Photosynthesis approached saturation in the range of light intensities used and light compensation point was reached at ca. 6-22 µM m-2 s-1 PAR, indicating that this species had a low light requirement for growth. Light stimulated shoot and root relative growth rates (RGR) but it was not related to ratios between root:shoot RGR. Laboratory observations indicated that (i) both shoot and root growth were simultaneously stimulated by light and (ii) the low light requirements of E. najas may explain its incidence in the Itaipu Reservoir, where...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egeria najas; Photosynthesis; Growth; Light compensation point; Rooted macrophytes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200011

Food Plants Eaten by Amazonian Manatees (Trichechus inunguis, Mammalia : Sirenia)
Provedor de dados: 52
Autores: Colares,Ioni G.; Colares,Elton P.
To determine the feeding habits of the Amazonian manatee Trichechus inunguis in some Central Amazonian rivers and lakes, we compared plant epidermis found in the stomach contents and/or faeces of animals with a reference collection of plants present in the studied areas. Twenty five samples from digestive tracts of animals found dead and 25 faeces samples found floating were analyzed. From these samples, 24 aquatic macrophytes were identified. The Gramineae family was identified in 96% of the samples, Paspalum repens and Echinochloa polystachya being the most abundant in the samples. The second most frequent family was the Pontederiaceae primarily Eichhornia crassipes. During the high water period, the animals showed a more selective diet (eight identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian manatee; Trichechus; Food; Plants eaten; Amazon.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100011

Four seasons abundance changes of Zalophus californianus californianus (Lesson 1828), Allen, 1880, in the Gulf of California, Mexico
Provedor de dados: 52
Autores: Maravilla-Chávez,M. Octavio; Zavala-González,Alfredo; Ortega-Rubio,Alfredo.
This paper describes for the first time the relative abundance, population structure and numeric variation of California sea lions in the Gulf of California, Mexico, during four different seasons. The relative abundance estimates varied from the highest 17,162 sea lions in July of 1990 (summer) to a minimum 7,470 in February of 1992 (winter), representing a reduction of 10,000 sea lions. Hypotheses about the migration of part of the population to the Pacific Ocean were compared and discussed against their residency inside the Gulf, and seasonal dispersion of individuals along this inner sea.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zalophus californianus californianus; Seasonal variations; Relative abundance; Migration; Gulf of California; Mexico.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100013

Studies on the morphometry of Macrobrachium nobilii (Decapoda, Palaemonidae)
Provedor de dados: 52
Autores: Mariappan,Pitchaimuthu; Balasundaram,Chellam.
Fourteen morphometric characters of Macrobrachium nobilii (Henderson and Matthai, 1910), a freshwater prawn were measured from 72 males and 66 females collected at random in field. The growth constant (b) of total length and weight for male (4.28) and female (3.02) differed significantly (P&lt;0.05). Comparison of slopes and elevations of regressions between sexes indicated significant difference in all the studied dependent parameters with carapace length (X), except with carapace width (Y). In males, major and minor chelipeds showed positive allometry while in females the growth was isometric.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allometry; Cheliped freshwater prawn length-weight Macrobrachium nobili.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300015

Properties of extruded xanthan-starch-clay nanocomposite films
Provedor de dados: 52
Autores: Melo,Cristina de; Garcia,Patrícia Salomão; Grossmann,Maria Victória Eiras; Yamashita,Fábio; Dall'Antônia,Luiz Henrique; Mali,Suzana.
The aim of this work was to manufacture the biodegradable nanocomposite films by extrusion from different combinations of cassava starch, xanthan gum and nanoclays (sodium montmorillonite - MMT- Na) and to characterize them according to their microstructure, optical, mechanical and barrier properties. Films were manufactured from nine starch/xanthan/nanoclay combinations, containing glycerol as plasticizer. Scanning electron microscopy (SEM) of the starch-xanthan extruded films showed reticulated surface and smooth interior, indicating that the gum was mostly concentrated on the surface of the films, while starch/xanthan/nanoclays films showed a more homogeneous surface, suggesting that the introduction of nanoclays provided a better biopolymeric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Film blowing; Cassava starch; Microstructure; Mechanical properties; Water vapor permeability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600019

SNPs genotyping technologies and their applications in farm animals breeding programs: review
Provedor de dados: 52
Autores: Koopaee,Hamed Kharrati; Koshkoiyeh,Ali Esmailizadeh.
Single nucleotide polymorphisms (SNPs) are DNA sequence variations that occur when a single nucleotide: adenine (A), thymine (T), cytosine (C) or guanine (G) in the genome sequence is altered. Traditional and high throughput methods are two main strategies for SNPs genotyping. The SNPs genotyping technologies provide powerful resources for animal breeding programs.Genomic selection using SNPs is a new tool for choosing the best breeding animals. In addition, the high density maps using SNPs can provide useful genetic tools to study quantitative traits genetic variations. There are many sources of SNPs and exhaustive numbers of methods of SNP detection to be considered. For many traits in farm animals, the rate of genetic improvement can be nearly doubled...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNPs genotyping; Traditional and high throughput methods; Animal breeding.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100013

Antimicrobial activity and mineral composition of shiitake mushrooms cultivated on agricultural waste
Provedor de dados: 52
Autores: Casaril,Kérley Braga Pereira Bento; Kasuya,Maria Catarina Megumi; Vanetti,Maria Cristina Dantas.
The antimicrobial activity and mineral composition of shiitake mushrooms were evaluated in four isolates of Lentinula edodes. Mushrooms were cultivated on artificial logs, based on eucalyptus sawdust enriched with 20% rice, wheat, or soybean bran, or combination of 10% of two of these supplements. The substrates were humidified with a 0.1% mate tea extract or water. Logs of Eucalyptus grandis were also used to cultivate the shiitake mushrooms. The antimicrobial activity of an aqueous extract, corresponding to 40 mg of mushroom dry matter, was in some cases, depending on the isolate, able to inhibit both Bacillus subtilis and Escherichia coli K-12, independent of substrate composition or the growth stage of the mushrooms. Nitrogen, phosphorus, potassium,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial property; Lentinula edodes; Mineral nutrients; Sawdust-based substrate; Mushrooms.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500017

Natural antioxidant from yerba maté (Ilex paraguariensis St. Hil.) prevents hamburger peroxidation
Provedor de dados: 52
Autores: Ferreira,Erica Lemos; Sampaio,Geni Rodrigues; Torres,Elizabeth Aparecida Ferraz da Silva; Bastos,Deborah Helena Markowicz.
The aim of this study was to evaluate whether yerba maté alcoholic extracts at very low concentrations (0.01 and 0.1%), prevent/retard lipid peroxidation in beef hamburgers without impairing sensory acceptability. For this TBARs and hexanal levels, fatty acid profile and cholesterol oxides were evaluated as oxidation parameters in beef hamburgers during 90 days' storage. The addition of 0.01% yerba maté ethanolic extracts proved inefficient in restraining the lipid peroxidation while the addition of 0.1% resulted in efficient antioxidant activity. Sensory evaluation of hamburger containing 0.1% yerba maté ethanolic extracts showed good acceptability. Yerba maté ethanolic extracts could entirely or partially replace the phenolic synthetic antioxidants in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba maté; Lipid oxidation; Natural antioxidants; Hamburger.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400021

Multicomponent complex formation between pyrimethamine, cyclodextrins and water-soluble polymers
Provedor de dados: 52
Autores: Rodrigues,Leticia Norma Carpentieri; Issa,Michelle Georges; Asbahr,Ana Carolina Cavassin; Silva,Marco Aurélio Carvalho; Ferraz,Humberto Gomes.
The combined effect of hydroxypropyl-β-cyclodextrin (HPβCD) and polyvinylpyrrolidone (PVP) or sodium carboxymethylcellulose (CMC) on the solubility of pyrimethamine (PYR) was studied. Equimolar PYR-HPβCD solid systems, in the presence or the absence of 0.25% (w/v) PVP or 0.10% (w/v) CMC were prepared by coevaporation or freeze-drying, and characterized by differential scanning calorimetry, X-ray diffraction analysis and dissolution profile. Phase-solubility analysis was used to investigate the interactions in aqueous solution between PYR and HPβCD, in the absence or presence of polymers, which showed a linear increase of PYR solubility depending on the concentration of HPβCD. The presence of polymer did not alter the stoichiometry of the complexes. DSC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrimethamine; HPβCD; Inclusion complexes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500014

Effect of Blanching on Enzyme Activity and Bioactive Compounds of Blackberry
Provedor de dados: 52
Autores: Noreña,Caciano Zapata; Rigon,Renata Trindade.
ABSTRACT The inactivation of peroxidase and polyphenol oxidase and the degradation of phenolic compounds, anthocyanins, and antioxidant activity in blackberry were investigated during blanching in water at 80-90 °C, and in steam, from 0 to 10 minutes. The enzyme kinetics indicated the presence of two isoenzymes with different thermal stability, following two-phase model, in which the reaction rate constant increased with increasing temperature for both the heat labile and heat resistant component. The phenolic compounds also followed a biphasic pattern, indicating a more heat resistant fraction, and the rate constants increased with increasing temperature. For the antioxidant activity and anthocyanins, the first order kinetic model (R2&gt;0.982) indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxidase; Polyphenol oxidase; Phenolic; Anthocyanins.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100501

Evaluation of Reference Genes for Quantitative PCR in Four Tissues from Rabbits with Hypercholesterolaemia
Provedor de dados: 52
Autores: Zhang,Zhen; Wen,Bin; Xu,Yuan; Jiang,En-ze; Liu,Jia-yu; Zhu,Ke-li; Ning,Fang-yong; Du,Zhi-Heng; Bai,Xiu-Juan.
Abstract Rabbit with hypercholesterolaemia is an important model for studying cholesterol metabolism disease. This study aimed to evaluate the expression stability of nine reference genes for quantitative PCR (qPCR) analysis in adrenal gland, liver, spleen, and kidney tissue from rabbits with hypercholesterolaemia. In total, 30 male Harbin Large White (HLW) rabbits were fed a normal feed (n = 15) or a high cholesterol feed (n = 15) for 8 weeks to induce hypercholesterolaemia. Nine reference genes were verified by qPCR using cDNA extracted from rabbit tissue samples. For qPCR analysis, reference genes were evaluated using the RefFinder and GeNorm algorithms. Overall, seven rabbits with hypercholesterolaemia were identified based on body weight and total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Evaluation; Reference genes; QPCR; Rabbits; Hypercholesterolaemia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100428

Microbial Synthesis and Characterization of Biodegradable Polyester Copolymers from Burkholderia Cepacia and Cupriavidus Necator Strains Using Crude Glycerol as Substrate
Provedor de dados: 52
Autores: Rodrigues,Plínio Ribeiro; Silvério,Tatiane Aparecida Barroso; Druzian,Janice Izabel.
Abstract Polymers are materials of pronounced importance in the modern world, since they are massively present in everyday life, especially in the form of packaging. However, most of these materials are derived from non-renewable sources and their disposal generates large volumes of waste, which is extremely damaging to natural environments. In this context, microbial biopolymers appear as a powerful alternative in the substitution of several applications of synthetic plastics, causing less harm to the environment, as they are biodegradable and produced from renewable sources. In this study, evaluation and characterization of polyhydroxyalkanoates (PHAs) produced by Cupriavidus necator (IPT 026 and IPT 027) and Burkholderia cepacia (IPT 119 and IPT 400),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crude glycerol; Biosynthesis; Polyhydroxyalkanoates; Characterization.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100209

Molecular epidemiology of the human group A rotavirus in the Paraná State, Brazil
Provedor de dados: 52
Autores: Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo.
From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008

Heregulin-1β Promotes the Synergistic Effect Between Allogenic Skin-Derived Precursor Differentiated Schwann Cells (SKP-SC) and Acellular Nerve Allograft (ANA) in Peripheral Nerve Regeneration Through Inhibiting Mir-21
Provedor de dados: 52
Autores: Wang,Houlei; Wu,Jingping; Zhang,Xinchao; Ding,Lei; Zeng,Qingmin.
Previous studies in our lab found that heregulin-1β with SKP-SCs (neurons and Schwann cells differentiated from SKPs) / ANA (acellular nerve allograft) transplantation represented a powerful therapeutic approach, and facilitates the efficacy of ANA in peripheral nerve injury. In this study, our purpose is to explore the mechanism between them. Firstly we transplanted ANA + SKP-SC + heregulin-1β into rats with right sciatic nerve injury and then detected the miR-21 and SOX2 (SRY-like HMG box 2) levels. Then we transfected miR-21 inhibitor in SCs (Schwann cells) which induced in hypoxic condition before harvesting. Then we detected expression of miR-21 and SOX2 using real time-PCR and western blot assay. Results in vivo showed that the expression of miR-21...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral nerve injury; ANA; SKP-SC; Heregulin-1β; MiR-21; SOX2.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100321

Plankton biomass in secondary ponds treating piggery waste
Provedor de dados: 52
Autores: Barthel,Lígia; Oliveira,Paulo Armando Victória de; Costa,Rejane Helena Ribeiro da.
This study aimed at analyzing the plankton biomass found in a piggery waste treatment system, composed of a high rate algal pond (HRAP), two maturation ponds (MP1, MP2) (System A) and a water hyacinth pond (WHP) (System B). The ponds were disposed in series and the study was performed for 32 weeks. The physicochemical variables monitored were: pH, temperature, dissolved oxygen, soluble chemical oxygen demand, nitrogen compounds and total phosphorus. The plankton biomass was identified at genus level and the ecology index was calculated so as to describe its development in the ponds. Results showed lower specific richness, which was associated to the mono-specific Chlorella sp population. The protozoa density was conversely proportional to the green algae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecologic index; Piggery waste; Plankton biomass; Ponds treatment.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600025

Application of the taguchi’s loss function in the breeding of nile tilapia fed with different levels of alcohol yeast
Provedor de dados: 52
Autores: Medri,Vandir; Pereira,Geni Varéa; Leonhardt,Júlio Hermann.
Studies were conducted to evaluate the effect of 10%, 20% and 30% ration substitutions for distillery yeast on the function Taguchi’s loss using two hundred and forty 45-day-old fingerlings of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial average weight of 1.25 ± 0.14 g placed in amianthus box.. The average results obtained for the limnological parameters of the water quality control through chemical analysis were considered normal. Taguchi’s losses for the total production, for the dead and discarded fishes did not show correlation for the different levels of alcohol yeast inclusion, showing that the choice of the yeast level in the ration for these fishes depended on its availability and occasional cost. It was observed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality; Ration; Taguchi; Tilapia; Yeast.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100011

Effects of betaine on the growth of the fish piauçu, Leporinus macrocephalus
Provedor de dados: 52
Autores: Normandes,Edivaldo Bento; Barreto,Rodrigo Egydio; Carvalho,Robson Francisco; Delicio,Helton Carlos.
In this study was tested the effect of betaine on the growth performance of the piauçu fish. For this, twenty-one fish (mean ± SD: body weight: 14.9 ± 1.3 g) were randomly distributed in one out of three experimental treatments (n = 7 each; 1 fish/aquarium): without betaine added in the food (control); 3.6 g betaine/Kg of food; 7.1 g betaine/Kg of food. The fish were exposed to experimental conditions for 60 days. Fish presented significant growth throughout experimentation, but no statistically significant difference was found for any parameter analyzed among the treatments, except for condition factor, which was higher in control group than betaine ones. Accordingly, it was concluded that betaine had no enhancing effect on growth of piauçu.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Betaine; Fish; Feeding behavior.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600010

Glucose isomerization in simulated moving bed reactor by Glucose isomerase
Provedor de dados: 52
Autores: Silva,Eduardo Alberto Borges da; Souza,Antônio Augusto Ulson de; Rodrigues,Alírio Egídio; Souza,Selene Maria Arruda Guelli Ulson de.
Studies were carried out on the production of high-fructose syrup by Simulated Moving Bed (SMB) technology. A mathematical model and numerical methodology were used to predict the behavior and performance of the simulated moving bed reactors and to verify some important aspects for application of this technology in the isomerization process. The developed algorithm used the strategy that considered equivalences between simulated moving bed reactors and true moving bed reactors. The kinetic parameters of the enzymatic reaction were obtained experimentally using discontinuous reactors by the Lineweaver-Burk technique. Mass transfer effects in the reaction conversion using the immobilized enzyme glucose isomerase were investigated. In the SMB reactive system,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simulated moving bed reactor; Glucose isomerization; Enzymatic kinetics; Mass transfer; Mathematical model; Dynamic simulation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400018

Physicochemical characterization of different trademarks of compound Yerba Maté and their herbs
Provedor de dados: 52
Autores: Scipioni,Griselda Patricia; Ferreyra,Darío Jorge; Schmalko,Miguel Eduardo.
The objectives of this study were to evaluate the physicochemical characteristics of the main herbs used in the mixture of yerba maté with other aromatic herbs and the characterization of the trademarks of compound yerba maté. Moisture, water extract, total ash, acid-insoluble ash and caffeine concentration were determined. Results showed higher values of moisture content, total and aci-insoluble ash and lower water extracts in the herbs. Determinations were carried out in nine trademarks of compound yerba maté. In most cases they complied with the standards of the country with the exception of one trademark from Argentina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba Maté; Herbs; Compound; Regulations; MERCOSUR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400020

Variability in the germination of spores among and within natural populations of the endangered tree fern Dicksonia sellowiana Hook. (Xaxim)
Provedor de dados: 52
Autores: Gomes,Gabriela Schmitz; Randi,Áurea Maria; Puchalski,Ângelo; Santos,Daisy da Silva; Reis,Maurício Sedrez dos.
The objective of this study was to analyze the existing variability in the germination of individuals' spores collected from two natural populations of Dicksonia sellowiana in southern Brazil. The largest intrapopulational variation was observed for the spores germination. These results suggested the existence of adaptative strategies that favored the gradual and continuous entrance of new individuals into the gametophytic population. The behavior of the individuals of the species within each population followed a similar pattern of initial development, in spite of conspicuous differences in the population structure. This showed a low differentiation between the populations and the existence of adaptative strategies which were common to both communities.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arborescent fern; Dicksonia sellowiana; Spore germination; Intraspecific variability; Adaptative strategies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100001

Effect of biological variables and capture period on the proximate composition and fatty acid composition of the dorsal muscle tissue of Hypophthalmus edentatus (Spix, 1829)
Provedor de dados: 52
Autores: Oliveira,Edna Regina Netto de; Agostinho,Angelo Antonio; Matsushita,Makoto.
This study analyzed the chemical composition of the dorsal muscle tissue of Hypophthalmus edentatus, in particular, fatty acids of the lipidic fraction, and its relationship with the biological variables and the capture period. The average values for moisture, ash, protein and total lipids were 63.15, 1.11, 14.27 and 17.24%, respectively. In the lipidic fraction, 35 fatty acids were identified, the most abundant being: 16:0, 18:1omega9, 18:0, 16:1omega7, 18:3omega3, DHA and EPA. The totals of respectively saturated, monounsaturated and polyunsaturated fatty acids were 41.15, 35.30 and 23.88%. The PUFA/SFA ratio was equal to 0.68 and the omega3/ omega6 ratio equal to 1.98. The PCA demonstrated that the long chain PUFAs contributed positively to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Fatty acids; Lipid contents; Proximate composition; Hypophthalmus edentatus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100015

The Effect of Water Parameters and Dissolved Minerals on the Hematological Parameters of during Breeding Period Catfish (Silurus glanis)
Provedor de dados: 52
Autores: Küçükgül,Azime; Yüngül,Mücahit; Kahraman,Zuhal; Dörücü,Mustafa.
Abstract In this study, the blood parameters of catfish (Silurus glanis Linnaeus, 1758) inhabiting Çelik Lake (Adıyaman, Turkey) were investigated. The study was conducted between April 2013 and October 2013. After obtaining a blood sample from each catfish has been hematologically analyzed. Then, the water temperature, dissolved oxygen, pH, calcium, magnesium and nitrate values were determined at before breeding (April), breeding period (June - July) and after breeding of fish (October) and the impacts of these data on hematological values were investigated. According to this study, it was observed that the hematological values obtained at before breeding period, breeding and after the breeding period were affected by the water quality parameters.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Çelik Lake; Hematological values; Reproduction; Water quality parameters.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100427

Effects of natural and anthropogenic environmental changes on riverine fish assemblages: a framework for ecological assessment of rivers
Provedor de dados: 52
Autores: Tejerina-Garro,Francisco Leonardo; Maldonado,Mabel; Ibañez,Carla; Pont,Didier; Roset,Nicolas; Oberdorff,Thierry.
Freshwater is a basic need for the mankind. Effective biological tools (ecologically based, efficient, rapid and consistently applicable to different ecological regions) are needed to measure the "health" of rivers. Adapting such tools over a broad geographic area requires a detailed understanding of both the patterns of organisms assemblage composition and distribution within and among water bodies under natural conditions, and the nature of the major environmental gradients that cause or explain these patterns. A comprehensive review of the available litterature dealing with the identification of environmental factors structuring riverine fish assemblages under natural conditions permits to identify the most consistent ones.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological indices; Fish community; Environmental parameters; Habitat.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100013

Falconiformes assemblages in a fragmented landscape of the Atlantic Forest in Southern Brazil
Provedor de dados: 52
Autores: Loures-Ribeiro,Alan; Anjos,Luiz dos.
An ecological analysis focused on distribution of Falconiformes, comprising abundance and morphology data, is provided. Samples were collected in a fragmented landscape within the Atlantic Rainforest, southern Brazil, between August and November 2001. Four main types of habitats were pinpointed among the 30 different sampling sites, while eight external morphological traits were employed. Twenty-one Falconiformes species were detected and jackknife estimates for regional richness reached 24.8 ± 2.56 species (p&lt;0.05). There were no differences between average number of diurnal birds of prey species in the different habitats under analysis (H-test, p&gt;0.05). Mantel's test for relative abundance and species morphology reveals weak association rates,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; Distribution; Falconiformes; Habitat analysis; Richness; Southern Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100018

An Alternative and Rapid Method for the Extraction of Nucleic Acids from Ixodid Ticks by Potassium Acetate Procedure
Provedor de dados: 52
Autores: Rodríguez,Islay; Fraga,Jorge; Noda,Angel Alberto; Mayet,Milagro; Duarte,Yanisia; Echevarria,Eduardo; Fernández,Carmen.
Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage of their life cycles were evaluated and compared with phenol-chloroform and ammonium hydroxide methods. The most rapid and most efficient variant was validated in the DNA extraction procedure from the engorged ticks collected from bovine, canine as well as from house ticks for the screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp. The ammonium hydroxide procedure was used for non-engorged ticks. All the variants were efficient and allowed obtaining PCR-quality material according to the specific amplification of 16S rRNA gene fragment of the original tick. DNA extracted from the ticks under the study was tested by multiplex PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ticks; PCR; DNA extraction.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400542

Projection of the Demand of Electricity in the State of Paraná for 2050 and Proposal of Complementarity of the Electrical Matrix through the Solar Photovoltaic Source
Provedor de dados: 52
Autores: Alves,Arileide Cristina; Yang,Renata Lautert; Tiepolo,Gerson Máximo.
ABSTRACT Energy is essential for human activities and, considering the prospect that the demand for energy increases, it is necessary to carry out studies of new technical solutions so as to make the use of the available sources feasible. Greater participation of renewable energy sources in the electricity matrix has been stimulated worldwide and a number of public policies have been adopted by different countries in order to seek greater energy security and sustainability. In this context, photovoltaic solar energy potentially plays an important role in the evolution of the participation of alternative sources in the world energy matrix, considering its abundance and wide availability in the terrestrial surface. This work proposes a methodology to model a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy Consumption 2050; Complementarity; Energy Planning; Photovoltaic Solar Energy; Mesoregions of Paraná.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200218

Biological Parameters of Euschistus heros (F.) (Heteroptera: Pentatomidae) and its Susceptibility to Entomopathogenic Fungi When Fed on Different Diets
Provedor de dados: 52
Autores: Oliveira,Daian Guilherme Pinto; Dudczak,Amnada Caroline; Alves,Luis Francisco Angeli; Sosa-Gomez,Daniel Ricardo.
ABSTRACT: The effects of six diets on biological parameters of Euschistus heros (F.) (Heteroptera: Pentatomidae) and its susceptibility to entomopathogenic fungi Beauveria bassiana (Bb) and Metarhizium anisopliae (Ma) were studied. The diets included a standard diet (bean pods+soybeans, peanuts and sunflower) and diets in which the bean pods were combined with only one type of grain, either soybean, peanut, sunflower, pea or sesame seeds. The best performance was that of insects fed on standard diet, which had the shortest development period and 49.5% of egg-to-adult viability. The insects fed the pea diet exhibited less than 30% viability and 11% of adults had wing deformations. The finite rate of increase values for the treatment groups were: standard:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beauveria bassiana Tri-trophic interactions Metarhizium anisopliae; Brown stink bug.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100509

Regra Prática Para a Limitação das Repetições nas Medidas Físico-Químicas
Provedor de dados: 52
Autores: Schwab,Arthur O.
In order to obtain a limitation of the number of repetitions in physico-chemical measurements, the author proposes formula 8 of this paper. delta is the difference between the maximum and minimum value of the measurements (form. 3) and A the approximation desired or imposed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physico-chemical measurements.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500009

A nonspecific primer anchored PCR technique for chromosome walking
Provedor de dados: 52
Autores: Zhang,Yan; Liu,Daqun; Yang,Wenxiang; Li,Yaning; Yan,Hongfei.
A chromosome walking method was improved in this work. The new method was named as nonspecific primer anchored PCR (NPA-PCR). Nested gene specific primers were designed based on the known region and long random primer using degeneracy oligonucleotides for nonspecific anchoring. Annealing temperatures were varied to control the priming. Target sequences were obtained by PCR with random primer and gene-specific primer. Nonspecific sequence with long random primers at both ends formed stem loop structure due to inverted terminal repeats. The method was employed to isolate a gene with newly-isolated actinomycin producing strain Streptomyces setonii Z-L-22. A 0.77 kb fragment of actinomycin synthetase gene cluster was isolated from the strain. The fragments of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nonspecific primer anchored PCR; Chromosome walking; Random primer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100013

Varietal identification of coffee seeds by RAPD technique
Provedor de dados: 52
Autores: Crochemore,Maria Lúcia; Nunes,Liliane Moreira; Andrade,Giselly Aparecida; Molinari,Hugo Bruno Correa; Vasconcellos,Maria Elizabeth.
This study aimed the identification of cultivars and/or lines of Coffea arabica of commercial interest, using PCR-RAPD markers. The DNA of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (Operon OPA 01, OPA 04, OPG 11, OPY 16, and OPX 09) were obtained from a selection of 56 primers. The electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of IAPAR59.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Cultivar identification; Molecular marker.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100002

Asynchronous Hatching and Extended Parental Care in Aegla schmitti (Decapoda, Anomura)
Provedor de dados: 52
Autores: Adam,Carolina de Lima; Marochi,Murilo; Lacerda,Mariana; Trevisan,Andre; Masunari,Setuko.
ABSTRACT The aim of this study was to describe the behavior of newly-hatched juveniles and maternal care in Aegla schmitti. The experiment was conducted using seven ovigerous females with eggs at different stages of embryonic development and 12 eggs that had been placed in a separate aquarium. We observed asynchronous hatching in all females, with a mean of 66 hatched juveniles per female. The females also exhibited extended maternal care, allowing the juveniles to remain inside their abdominal chamber or on other parts of their body in the first few days after hatching. The juveniles that hatched in a separate aquarium fed on the remaining eggs, despite food being available. The incubation period and number of hatched eggs are highly variable between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacean behavior; Maternal care; Hatching period; Aeglidae.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100450

Development of an Extraction Protocol for Fetal Sexing from the Fourth Week of Gestation by Real Time PCR
Provedor de dados: 52
Autores: Oliveira,Edwin José Torres de; Rabacow,Ana Paula Maluf; Figueiredo,Bruno Pereira de; Ishikawa,Raíssa Borges; Pessatto,Lucas Roberto; Pelizaro,Bruno Ivo; Figueiredo,Helder Pereira de; Oliveira,Rodrigo Juliano.
Abstract Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was performed employing an in-house method based on guanidine thiocyanate and magnetic bead. For the amplification it was used the Quantifier Y Kit™. The fetal sexing analysis of the 132 pregnant women were 100% in agreement with the ultrasound. Sensitivity and specificity for detection of Y chromosome sequences was possible using Real-Time Polymerase Chain Reaction since the 4th week of gestation. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fetal-sexing; DNA extraction; RtPCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100416

Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)
Provedor de dados: 52
Autores: Sarubbo,Leonie Asfora; Oliveira,Luciana Alves de; Porto,Ana Lúcia Figueiredo; Campos-Takaki,Galba Maria de; Tambourgi,Elias Basile.
The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aqueous two-phase systems; Ine serum albumin; Cashew-nut tree gum; Protein partition.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003

Phytochemical and molluscicidal activity of Mikania glomerata Sprengel (Asteraceae) in different lifestages of Subulina octona (Mollusca, Subulinidade)
Provedor de dados: 52
Autores: Souza,Bruna Aparecida de; Silva,Lidiane Cristina da; Chicarino,Evelyn Durço; Bessa,Elisabeth Cristina de Almeida.
The objective of this work was to study the molluscicidal activity of aqueous extract of Mikania glomerata on the land snail Subulina octona and to assess the changes caused on the snail's fecundity, growth, hatchability and the offspring produced after exposure. Eggs, newly hatched and 30 day old snails were exposed to the sublethal concentration of M. glomerata extract for 24 and 48 h and the observations were made for 120 days. The presence of tannins, flavonoids and saponins were shown by phytochemical tests. The extract reduced significantly the hatchability and the survival of the offspring hatched from the exposed eggs. The eggs exposed for 48 h presented lower hatchability rates. There was a reduction on survival and growth of newly hatched and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Land snail; Guaco; Molluscicidal; Toxicity; Control.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200015

Interbular space characterization in adult capybara (Hydrochoerus hydrochaeris) testis
Provedor de dados: 52
Autores: Paula,Tarcízio Antônio Rego de; Matta,Sérgio Luiz Pinto da; Silva Jr.,Valdemiro Amaro da; Costa,Deiler Sampaio; Fonseca,Cláudio Cesar; Neves,Marco Túlio David das.
This study aimed at the qualitative and quantitative characterization of the different intertubular tissue components in adult male capybara. Testes of 15 adult capybaras from a commercial slaughterhouse of wild animals were used. The average percentage of Leydig cells was 32.95 ± 10.03 %, whereas in the other components of the intertubular space it was 14.95 ± 4.79 %, thus the whole intertubular space totalized 47.91 ± 11.48 %. The average weight of the testes were 32.83 ± 4.78g, while the animals' body weight were around 52.37 kg and the gonadosomatic and leydigosomatic indexes were 0.125 ± 0.02% and 0.0411 ± 0.0148% respectively. It was concluded that the leydigosomatic index of the capybaras was lower than the one observed in rats, as well as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intertubular space; Leydig cell; Testis; Capybara; Hydrochoerus hydrochaeris.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200014

In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia
Provedor de dados: 52
Autores: Razak,Ummi Nur Ain Abdul; Ong,Chong Boon; Yu,Tiew Sing; Lau,Li Kiaw.
Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP) and Kinetin (Kn) for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and 1-Naphthaleneacetic acid (NAA) for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants) was observed on a Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 Kn. The maximum number of roots (30.12 ± 2.1 roots per explants) was obtained on a MS medium containing 1.0 mg L-1 IBA. The well rooted plantlets were successfully weaned and acclimatized in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MS medium; Non-caloric sweetener; Proliferation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100004

Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
Provedor de dados: 52
Autores: Vestri,Sandro; Nikami,Hideki; Saito,Masayuki; Machado,Ubiratan F.
In the present study, the production of an anti-GLUT 2 antibody is reported. The antibody (S-5096) was raised in New Zealand rabbits immunized with an immunogen prepared by cross-linking the synthetic peptide and a carrier protein. A COOH-terminal decapeptide of rat GLUT 2 predicted sequence was synthesized and coupled to keyhole limpet hemocyanin, using the glutaraldehyde method. Subcellular membrane fractions were prepared from renal cortex (C) and medulla (M), and subjected to Western blotting analysis using the antiserum in a 1:200 dilution and, subsequently, [125I]protein A. As the results showed, the S-5096 anti-serum showed clear blots in kidney samples, stronger in cortex than in medulla as expected, whereas white adipose tissue and heart samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose transporter; GLUT 2; Antibody; Coupling peptide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200004

Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
Provedor de dados: 52
Autores: Vieira,Felipe Almeida; Cunha,Maura da; Klein,Denise Espellet; Carvalho,André de Oliveira; Gomes,Valdirene Moreira.
In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-1; 3-glucanase PR protein chitinases secretory structure.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700004

Models for the estimation of the physical quality of a Yellow Red Latosol (Oxisol) under pasture
Provedor de dados: 52
Autores: Ribon,Adriana Aparecida; Tavares Filho,João.
Models were developed considering the physical parameters penetration resistance (RP), bulk density (Ds), gravimetric moisture (U), clay content (Ag), sand content (Ar) and organic matter (MO) collected from a Yellow Red Latosol (Oxissol) medium texture under pasture from Rondonópolis county district, Mato Grosso State. Sampling was carried out in 34 fully randomized points in a 1 ha area to the depth of 0-20cm. The models were created using the Statistical Analysis System program (SAS) through which linear multiple regressions and correlations were made. Using the determination coefficient the following model was selected: RP=-12.134+0.004MO+7.6187Ds+0.131AG (R²=0.78). The evaluation of the organic matter and the soil clay content were important tools to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Modeling; Penetration resistance; Organic matter; Texture.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100004

Antimicrobial activity of Syagrus coronata (Martius) Beccari
Provedor de dados: 52
Autores: Hughes,Alice Ferreira da Silva; Lima,Fabíola Guedes de; Lucchese,Angélica Maria; Góes Neto,Aristóteles; Uetanabaro,Ana Paula Trovatti.
This work aimed to evaluate the antimicrobial activity of aqueous and methanol extracts of leaves, inflorescences, nut-shell, liquid and solid endosperm nuts of Syagrus coronata against pathogenic bacteria and yeast. Screening was initially performed using the agar dilution method. The extracts regarded as bioactive underwent liquid-liquid partition for determination of their minimum inhibitory concentration and minimum bactericide concentration (MIC and MBC) and those of their respective fractions against the microorganisms inhibited in preliminary tests. Antimicrobial activity was observed only in inflorescences. The corresponding aqueous extract was effective against B. cereus and the three strains of S. aureus, and the corresponding MIC and MCB values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Syagrus coronata; Arecaceae; Antimicrobial activity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200012

Floating Aquatic Macrophytes Decrease the Methane Concentration in the Water Column of a Tropical Coastal Lagoon: Implications for Methane Oxidation and Emission
Provedor de dados: 52
Autores: Fonseca,André Luiz dos Santos; Marinho,Claudio Cardoso; Esteves,Franscisco de Assis.
ABSTRACT In wetlands, the knowledge accumulated on the role of aquatic plants in the methane cycle focused on emergent macrophytes, to the detriment of other typologies. Herein, we evaluated whether the free-floating macrophyte Salvinia auriculata Aubl. and the floating-leaved macrophyte Eichhornia azurea (Sw.) Kunth. decrease the water column methane concentrations compared to a plant-free surface. We prepared microcosms by inserting an individual of S. auriculata or of E. azurea into chambers filled with lagoon water previously bubbled with CH4. Another set of chambers was incubated only with the prepared water, representing the plant-free surface. Half of the chambers were kept in the dark and half in sunlight to simulate a diel cycle. We observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methane; Eutrophication; Global warming; Eichhornia azurea; Salvinia auriculata.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100702

Evaluation of the Blue Crab Callinectes sapidus Rathbun, 1896 Bycatch in Artisanal Fisheries in Southern Brazil
Provedor de dados: 52
Autores: Ruas,Vinicius Mendes; Becker,Cintia; D’Incao,Fernando.
ABSTRACT   We evaluated the incidental catch of the blue crab in artisanal fisheries through the analysis of sex ratio, catch per unit effort, net positioning, and size in which the capture probability was 50% (P50). The specimens were collected from 2011 to 2013 in the estuary of Patos Lagoon, southern Brazil. The analysis showed a higher occurrence of males. Variations in the catch per unit effort showed greatest abundance values close to the mouth of the estuary. Catch per unit effort was similar when comparing fishing seasons and net positions. P50 indicated a low selectivity of the nets since it varied between studied areas but stayed below the size at first maturity. Catches indicated a greater effort on males, since females migrated to spawn in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachyura; Fishery resource; Patos Lagoon; Fyke net; Estuary.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100448

Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari
Provedor de dados: 52
Autores: Santos Filho,Plinio Rodrigues dos; Santos,Breno Régis; Barbosa,Sandro; Vieira,Letícia Rios; Freitas,Natália Chagas de; Dias,Daniele Ferreira; Santos,Marcelo Henrique dos.
Garcinia brasiliensis, popularly known as Bacupari, is native to the Amazon and commonly used in folk medicine for its therapeutic properties. This plant is rich in bioactive compounds like benzophenones. However, there are no works about the in vitro establishment and achievement of secondary metabolites in this plant. Thus, the aim of this work was to determine the growth curve and to perform the biochemical and phytochemical analyses in calli obtained from the procambium segments of Bacupari. The growth curve of calli followed a sigmoidal pattern, with four distinct phases (lag, exponential, linear, deceleration). Total soluble sugars were higher on the inoculation day and the reducing sugars on the 20 th day. Amino acids increased from the 60 th day up...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacupari; Benzophenones; Calli.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300003

The Psychophysiological Responses of the Chronic Ischemic Stroke Patients to the Acute Stress were Changed
Provedor de dados: 52
Autores: Mirzaee,Omid; Saneian,Mojtaba; Vani,Javad Rasouli; Shahrivar,Kamyab; Peyravi,Mahmoodreza; Shariat,Abdolhamid; Hatef,Boshra.
Abstract: Mood disorder is one of the complications of stroke. The inability to cope with stress is also a prognosis of depression and anxiety. The aim of this study is to assess the response of stress system in the post stroke patients. Twelve healthy controls (HC) and twelve post-stroke patients after filling in the State-Trait Anxiety Inventory completed the Trier Social Stress Test (TSST) which induces acute stress. Salivary samples were collected to determine salivary cortisol levels and ECG record were taken in four times (before, right after stress, after two recoveries: 20 and 40 minutes after stress). ECG was also recorded during TSST and then the linear and non-linear features of Heart Rate Variability (HRV) were analyzed. The results showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemic stroke; Acute social stress; Cortisol; Heart rate variation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100312

Allelopathic evidence in exotic guava (Psidium guajava L.)
Provedor de dados: 52
Autores: Chapla,Tatiani Elisa; Campos,João Batista.
The allelopathic activity of the leaf aqueous extract of guava (Psidium guajava L.) on seed germination and root growth in lettuce (Lactuca sativa L.) was studied in an experiment consisting of four replicates with 10 seeds each and extract concentrations of 5, 10, and 20%. Guava allelopathic activity occurred during the germination and growth of lettuce roots at 20% extract concentration, as shown by ANOVA. This result suggested the allelopathic potential of the species. Therefore, the success of P. guajava in invading environments could be partially due to allelopathy. Further studies using other plant parts and field experiments should be conducted.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abandoned pastures; Allelopathy; Biological invasion; Riparian vegetation; Seeds.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600012

Sodium Chloride-Induced leaf senescence in Hydrocotyle bonariensis Lam. and Foeniculum vulgare L.
Provedor de dados: 52
Autores: Haddad,Claudia R. B.; Mazzafera,Paulo.
Leaf senescence induced by sodium chloride was studied in Hydrocotyle bonariensis Lam. and Foeniculum vulgare L. Both species belong to Umbelliferae family, however only H. bonariensis grows spontaneously in sandy soils of coastal regions (saline soils). Leaves of plants receiving nutrient solutions containing different concentrations of NaCl were evaluated for fresh and dry weights and chlorophyll content. The denaturating electrophoretic profiles of leaf proteins were also studied. Sodium chloride changed the protein profile of F. vulgare and hastened the leaf senescence of both the species. However, plants of H. bonariensis receiving 599 mM NaCl lasted longer than F. vulgare. Therefore, the occurrence of H. bonariensis in saline soils might be related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorophyll; Protein; Salinity; Senescence.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000200005

Adult feeding and mating effects on the biological potential and parasitism of Trichogramma pretiosum and T. acacioi (Hym.: Trichogrammatidae)
Provedor de dados: 52
Autores: Pratissoli,Dirceu; Oliveira,Harley Nonato de; Polanczyk,Ricardo Antonio; Holtz,Anderson Mathias; Bueno,Regiane Cristina Oliveira de Freitas; Bueno,Adeney de Freitas; Gonçalvez,José Roberto.
This work was carried out to study the effects of adult feeding and mating on the biological potential and parasitism of Trichogramma pretiosum and T. acacioi (Hymenoptera: Trichogrammatidae) to improve their use in biological control programs. Both species presented higher parasitism and longevity whenever adults were fed. Fed and unmated T. pretiosum females led to low parasitism whereas T. acacioi females did not present parasitism whatsoever. Egg viability of T. pretiosum was similar for fed and mated individuals, but T acacioi showed lower values for this parameter when unfed and without mating. Unmated females produced only males while mated ones had more than 60% female descendents for both Trichogramma species. Therefore, mated and fed female...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Parasitoid feeding; Parasitoid mating.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500001

Public Policies to Be Implemented to Encourage the Use of Renewable Energy Sources in the Rural Area of Paraná
Provedor de dados: 52
Autores: Batista,Alexandre G.; Betini,Roberto C.; Bareta,Maria Luiza E.; Sandim,Álvaro A.; Pepplow,Luiz A.
Abstract Around the world there’s a rising interest in the installation of renewable energy sources due to their ecological, economical, political and social advantages. In this article is studied specifically the case of the incentive towards the installation of these sources in the state of Parana’s rural area, where they wouldn’t just be ecologically interesting, but also would help the improvement on the living conditions of the rural producers, promote income distribution and would cause an increase on the power supply, this way reducing the tariff. However, the problem arises in the acquisition of the energy generator, since even though they are interesting for the rural producers, these sources are majorly too expensive and end up being beyond their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renewable energy; Public policies; Rural areas.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200219

Late Holocene Vegetation History and Early Evidence of Araucaria angustifolia in Caçapava do Sul in the Lowland Region of Rio Grande do Sul State, Southern Brazil
Provedor de dados: 52
Autores: Behling,Hermann; Verissimo,Nuno; Bauermann,Soraia; Bordignon,Sergio; Evaldt,Andreia.
Little is known about the southernmost occurrence of small areas with Araucaria angustifolia populations in Caçapava do Sul in low elevated areas of Rio Grande do Sul State, about 130 km to the south of to the highlands of southern Brazil where the main distribution of Araucaria is found. This occurrence is about 130 km further south to the main area of Araucaria angustifolia which is on the highlands in southern Brazil. The question is whether this occurrence is natural, due to indigenous peoples, or due to plantation by post-Columbian settlers. To trace the origin of this little known southernmost existence of Araucaria angustifolia trees is of particular interest for conservation issues. To address this question we did a vegetation survey and studied a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Late Holocene; Araucaria angustifolia; Pollen analysis; Southern Brazil.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100700

The impact of food manufacturing practices on food borne diseases
Provedor de dados: 52
Autores: Sousa,Cristina Paiva de.
Food-borne illness is a major international problem and an important cause of reduced economic growth. The contamination of the food supply with the pathogens and its persistence, growth, multiplication and/or toxin production has emerged as an important public health concern. Most of these problems could be controlled with the efforts on the part of the food handlers, whether in a processing plant, a restaurant, and others. In contrast with most chemical hazardous compounds, the concentration of food pathogens changes during the processing, storage, and meal preparation, making it difficult to estimate the number of the microorganisms or the concentration of their toxins at the time of ingestion by the consumer. This review shows main microorganisms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food-borne diseases; Staphylococcus spp.; Escherichia coli; Salmonella spp.; Food handlers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400020

Thermoanalytical and starch content evaluation of cassava bagasse as agro-industrial residue
Provedor de dados: 52
Autores: Lacerda,Luiz Gustavo; Almeida,Rafael Ramires; Demiate,Ivo Mottin; Carvalho Filho,Marco Aurélio Silva; Vasconcelos,Eliane Carvalho; Woiciechowski,Adenise Lorenci; Bannach,Gilbert; Schnitzler,Egon; Soccol,Carlos Ricardo.
Starch nutritional fractions as well as thermal properties and other analysis are essential for food and industrial application. Cassava bagasse is an important agro-industrial residue and its starch content was evaluated using two alternative methods. Thermal characterization and microscopy analyses helped to understand how hydrolysis digests starchy fraction of cassava bagasse. The melting point of cassava starch occurred at 169.2ºC. Regarding TG analyses, after moisture content, there were observed two main mass losses for all samples. Results suggest hydrolysis carried out using enzyme is less effective in order to convert total starch content in cassava bagasse. However, using sulfuric acid, fibers are affected by analyses conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylase; Cassava; Hydrolysis; Thermal analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700019

Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
Provedor de dados: 52
Autores: Zaia,Dimas Augusto Morozin; Marques,Fábio Rangel; Zaia,Cássia Thaïs Bussamra Vieira.
A comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p&gt;0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total proteins; Spectrophotometry; Blood plasma; Dye-binding methods.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300008

Radiolabeled PNAs for imaging gene expression
Provedor de dados: 52
Autores: Wickstrom,Eric; Sauter,Edward; Tian,Xianben; Rao,Sampath; Quin,Weyng; Thakur,Mathew.
Scintigraphic imaging of gene expression in vivo by non-invasive means could precisely direct physicians to appropriate intervention at the onset of disease and could contribute extensively to the management of patients. However, no method is currently available to image specific overexpressed oncogene mRNAs in vivo by scintigraphic imaging. Nevertheless, we have observed that Tc-99m-peptides can delineate tumors, and that PNA-peptides are specific for receptors on malignant cells and are taken up specifically and concentrated in nuclei. We hypothesize that antisense Tc-99m-PNA-peptides will be taken up by human breast cancer cells, hybridize to complementary mRNA targets, and permit imaging of oncogene mRNAs in human breast cancer xenografts in a mouse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiolabeled PNAs; Scintigraphy imaging; Gene expression.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500008

Optimization of levan production by Zymomonas mobilis
Provedor de dados: 52
Autores: Ananthalakshmy,V. K; Gunasekaran,P.
Effect of different fermentation conditions on levan production by Zymomonas mobilis B-4286 was studied. Levan production increased from 5.7-g/l to 12.6-g/l with an increase in initial sucrose concentration (50-150 g/l). Above 15% (20 and 25%) sucrose concentration, there was no increase in the biomass. The sucrose hydrolysis and levan production occurred even in the absence of significant growth of cells. Maximum amount of levan was produced (14.5 g/l) at pH 5 and 15 g /l at 25(0)C temperature. At temperature between 35(0)C and 40(0)C, levan production was not detected. Presence of glucose in the medium considerably reduced levan production (2.8 g/l) than fructose 6.7g/l.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levan; Levansucrase; Sucrase; Zymomonas mobilis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300004

Evaluation of Two Fitting Methods Applied for Thin-Layer Drying of Cape Gooseberry Fruits
Provedor de dados: 52
Autores: Karacabey,Erkan.
ABSTRACT Drying data of cape gooseberry was used to compare two fitting methods: namely 2-step and 1-step methods. Literature data was also used to confirm the results. To demonstrate the applicability of these methods, two primary models (Page, Two-term-exponential) were selected. Linear equation was used as secondary model. As well-known from the previous modelling studies on drying, 2-step method required at least two regressions: One is primary model and one is secondary (if you have only one environmental condition such as temperature). On the other hand, one regression was enough for 1-step method. Although previous studies on kinetic modelling of drying of foods were based on 2-step method, this study indicated that 1-step method may also be a good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1-step &amp;amp; 2-step method kinetic modelling Page model Two-term exponential model food processing aspects.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100427

Short-term cold storage of sperm from six neotropical characiformes fishes
Provedor de dados: 52
Autores: Marques,Simone; Godinho,Hugo Pereira.
Sperm of the following Neotropical Characiformes fish species were tested for cold storage: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus and Prochilodus marggravii. Each sperm sample was split into two aliquots. The first was placed into a plastic bag with air or oxygen and the second, in a plastic tube with air. The samples were maintained at temperatures between 1.7-4.9 ºC. The rate of sperm motility was estimated using a 50 mM NaCl solution as the activating solution. The shortest sperm storage duration (7 h) was recorded for L. friderici, when the sperm motility rate reached ~ 30%, whereas the longest duration (20 h) was obtained with the sperm of P. lineatus. A fertilisation test using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cool storage of sperm; Sperm motility; Semen; Neotropical fishes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500016

Computational algorithm for stability analysis of distribution networks with distributed generation
Provedor de dados: 52
Autores: Weigert,Gabriela Rosalee; Gurski,Erico; Benedito,Raphael Augusto de Souza.
Abstract With the insertion of distributed generation in distribution networks, security analysis becomes crucial, in islanded operation or when the system is connected to the grid. Dynamic stability analyses are necessary in order to achieve minimum levels of security and reliability. Such analyses are obtained through the solution of the algebraic-differential set of equations that model the distribution grid and their generators, which requires numerical solution methods. Therefore, this paper presents a computational tool for transient stability and large disturbance frequency stability analysis, considering distribution systems with the insertion of biomass-fed distributed generation, which may, or may not, have speed regulation systems. The presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distributed generation; Biomass; Dynamic stability; Speed regulator; Islanded operation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200209

Use of chitosan gel for the purification of protein
Provedor de dados: 52
Autores: Tang,ZhenXing; Qian,JunQing.
The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitosan; Gel; Preparation; Application.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200015

Morphological aspects of the propagation in Heliconia velloziana L. Emygd. (Zingiberales: Heliconiaceae)
Provedor de dados: 52
Autores: Simão,Daniela G.; Scatena,Vera L.
Morphology of seed and vegetative propagation was studied toHeliconia velloziana L. Emygd. (Heliconiaceae), a rhizomatous herb with a large aerial part. Seeds took about four months to germinate, probably due to the undifferentiated embryo and hard endocarp. Seedlings had two first foliar structures as scale leaves and the primary root developed, but the adventitious roots were more conspicuous. Vegetative propagation by rhizomes was faster than the sexual one. The roots developed after one month, while young shoots started developing after four to six weeks; young aerial shoots presented a variable number of cataphylls and two protophylls.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Heliconia; Rhizomes; Seedlings; Seed propagation; Vegetative propagation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100011

High metal contents in coffee plant organs developed in tubets with different proportions of biosolid composts and carbonized rice hulls
Provedor de dados: 52
Autores: Franco,Elias; Miglioranza,Edison; Fonseca,Ésio de Pádua.
The objective of this study was to analyze the effect of biosolid composts (BC) and carbonized rice hull (RH) on the production of Coffea arabica L. cv Catuaí Vermelho seedlings (IAC H 2077-2-5-99) grown in tubets. A totally randomized experimental design with five treatments and four replications was conducted in a nursery, to evaluate Co, Ni, Cr, Pb, Cu, Mn and Zn contents in roots, stems and leaves. Higher contents of Cr, Co, Ni, Pb, Cu and Zn were found in roots, while leaves carried the highest content of Mn. Zn in leaves went from average to high in plants developed in BC. In plants developed in RH. Mn content was about four times higher than the adequate concentration. Ni, Co, Cr, Pb and Cu contents found in leaves were considered normal, according...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica L.; Substrate; Sewage sludge; Coffee nutrition; Coffee seedling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400002

Study of Acari and Collembola Populations in Four Cultivation Systems in Dourados - MS
Provedor de dados: 52
Autores: Mussury,Rosilda Mara; Scalon,Silvana de Paula Quintão; Silva,Sandra Verza da; Soligo,Valdirene Regis.
The impact four cultivation systems on the soil fauna was studied, using Oribatida and Gamasida acarids as bioindicators and collembolan. The research was carried out in experimental fields, located in EMBRAPA - CPAO in Dourados, Centerwest of Brazil from July 1997 to December 1999. The constant pasture system presented smaller impact on the soil fauna followed by agricultural cattle rotation and a direct plantation system. In the conventional plantation series, the populational density of the mesofauna organisms was low, especially collembolan families.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edafica mesofauna; Direct plantation; Soil prepare systems; Cultures rotation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300002

Co-Infection by Sarcoptes scabiei and Microsporum gypseum in Free-Ranging Crab-Eating Fox, Cerdocyon thous (Linnaeus, 1766).
Provedor de dados: 52
Autores: Almeida,Jonatas Campos de; Leal,Carlos Adriano de Santana; Melo,Renata Pimentel Bandeira de; Albuquerque,Pedro Paulo Feitosa de; Pedrosa,Camila de Morais; Zermiani,Fabiana Correa; Farias,Roberto Citelli de; Mota,Rinaldo Aparecido.
ABSTRACT The aim of the present study was to describe the clinical manifestation, treatment and outcome of a case of co- infection by Sarcoptes scabiei and Microsporum gypseum in Cerdocyon thous (crab-eating fox) from Northeastern Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatophytes; Public health; Sarcoptic mange; Wild canid; Zoonose.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100313

Heavy Metal Bioaccumulation in Enteromorpha intestinalis, (L.) Nees, a Macrophytic Algae: The Example of Kadin Creek (Western Anatolia)
Provedor de dados: 52
Autores: Yozukmaz,Aykut; Yabanli,Murat; Sel,Fatma.
ABSTRACT This study aims to specify the amount of ten heavy metals (Al, Cr, Mn, Ni, Cu, Zn, As, Cd, Hg, Pb) in water, sediment and Enteromorpha intestinalis samples gathered from Kadin Creek in seasonal periods in 2011 and 2012 and to reveal bioaccumulation capability of this macrophytic algae. In this regard, heavy metal concentrations in the gathered samples were determined first with mineralization and then via inductively coupled plasma mass spectroscopy (ICP-MS) method. Findings suggested that algae could be used as an indicator in freshwater ecosystems in terms of studied heavy metals. With regard to bioconcentration factor values, in terms of water, algae accumulated aluminum the most and cadmium the least (Al &gt; Cu &gt; As &gt; Zn &gt; Cr &gt; Mn...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metals; Enteromorpha intestinalis; Kadın Creek.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100701

Fauna living in colonies of Mussismilia hispida (Verrill) (Cnidaria: Scleractinia) in four South-eastern Brazil islands
Provedor de dados: 52
Autores: Nogueira,João Miguel de Matos.
Colonies of Mussismilia hispida were collected in four south-eastern Brazil islands and fixed in formalin. Volume, living and basal areas of each coral head were measured. Animals found over, under and inside the corals were sorted, resulting in 9657 specimens belonging to more than 130 taxa. The underside of the colonies was occupied by bivalves and colonial forms of sponges, bryozoans and ascidians. On the living surface, barnacles and cryptochiridean crabs were detected. The endolithic animals included polychaetes, bivalves and sipunculid worms. Most of the animals collected were vagile microcrustaceans. In general, the corals in this ecosystem: (1) act as a nursery place for the community; (2) provide protection for many species also found in other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Associated fauna; Community ecology; Corals; Mussismilia hispida; South-eastern Brazil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300014

Biodosimetry for dose assessment of partial-body exposure: a methodological improvement
Provedor de dados: 52
Autores: Fernandes,Thiago Salazar; Loyd,David C.; Amaral,Ademir.
This study has explored the possibility of combining culture times with extending the duration for which Colcemid is present in cell culture in order to obtain better dose estimations following partial-body exposures. Irradiated and unirradiated blood was mixed to simulate a partial-exposure. Dicentric frequencies and resultant dose estimations were compared from 48 and 72 h cultures with Colcemid added at the beginning, after 24 h or for the final 3 h. The frequencies of dicentrics in first division cells increased with the cell culture time, providing better dose estimations. Unwanted excessive contraction of chromosomes caused by prolonged contact with Colcemid was measured and ways to avoid this are discussed. It is suggested that the combination of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodosimetry; Partial-body exposure; Dicentrics; Chromosome contraction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700016

Evaluation of Global Heating Reduction Potential with the Replacement of Electricity Supplied by the Local Concessionaire Via Solar Renewable Source.
Provedor de dados: 52
Autores: Pepplow,Luiz Amilton; Silva,Vander Luiz da; Betini,Roberto C.; Pereira,Thulio C. G.
Abstract The objective of this paper is to evaluate, through the 100-year Life Cycle Analysis, Global Warming Potential (GWP) and Global Temperature Increasing Potential (GTP) impact categories. It is proposed scenario of replacement of the energy matrix for the supply of electricity by Solar Photovoltaic, adopting as consumption base the facilities of Supermarkets, as they contribute to environmental impacts for greenhouse gas emissions such as: intensive use of electricity, occupation large areas such as the main building or parking lot sealing the ground, the movement of customers' vehicles with air and noise pollution and the use of plastic bags. Modifying the energy matrix using electricity from renewable sources enables the reduction of greenhouse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renewable energy; Life cycle assessment; Environmental impact.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200202

Start-up and performance characteristics of a trickle bed reactor degrading toluene
Provedor de dados: 52
Autores: Misiaczek,Ondrej; Paca,Jan; Halecký,Martin; Gerrard,Andrew Mark; Sobotka,Miroslav; Soccol,Carlos Riccardo.
The objective of this work was to evaluate toluene degradation in a trickle bed reactor when the loading was carried out by changing the air flow rate. The biofiltration system was inoculated with a mixed microbial population, adapted to degradation of hydrophobic compounds. Polypropylene high flow rings were used as a packing material. The system was operated for a period of 50 days at empty bed residence times ranging from 106s to 13s and with a constant inlet concentration of toluene of 100 mg.m-3. The reactor showed high removal efficiency at higher contact times and increasing elimination capacity with higher air-flow rates. The highest EC value reached was 9.8 gC.m-3.h-1 at EBRT = 13s. During the experiment, the consumption of NaOH solution was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotrickling filter; Constructed mixed population; Toluene; Air flow rate loading.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500015

Influence of ethanol concentration, addition of spices extract, and level of sweetness on physico-chemical characteristics and sensory quality of apple vermouth
Provedor de dados: 52
Autores: Joshi,Vinod K.; Sandhu,Danwant K.
The composition of apple base wine was found to be suitable for conversion into vermouth. The spices extract contained more TSS, tannins, esters, volatile acid but lower titrable acid than apple base wine. To optimize and develop apple vermouth with different ethanol concentrations (12%, 15%, 18%), sugar content (4%, 8%) and spices extract (2.5% and 5.0%) was prepared and was evaluated. Significant differences in physico-chemical characteristics and sensory quality amongst the vermouths having different levels of alcohol, sugar and spices extract were noted. Generally, increasing ethanol content decreased titrable acidity, tannins, macroelements and colour units while micro-elements, viscosity and total esters were increased. Increased sugar level affected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple; Vermouth; Ethanol spices extract; Sweetness; Esters; Quality; Base wine; Mineral; Sensory.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500015

Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells
Provedor de dados: 52
Autores: Teng,Yong; Hu,YunYu; Li,Xu Sheng; Wang,Zhen; Wang,Rui.
Primary human bone marrow stromal cells (hMSCs) were transfected with human telomerase reverse transcriptase (hTERT) gene with lipofection method. The hTERT transfected hMSCs of passage 100 underwent chondrogenesis induction with dexamethasone, transforming the growth factor β and vitamin C, osteogenesis induction with dexamethasone, β glycerophosphoric acid and vitamin C, and cardiomyocyte induction with 5-azacytidine. After 7, 14, 21 and 28 days of induction, immunocytochemistry was performed to detect the expressions of type I and II collagen and osteocalcin, and alizarin red staining was performed to detect the bone nodule formation in osteogenesis induction. Immunocytochemistry was carried out to detect the striated muscle actin expression in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transfection; Cell immortalization; Telomerase; Bone marrow stromal cells.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100006

Osteoarthritis in horses - Part 2: a review of the intra-articular use of corticosteroids as a method of treatment
Provedor de dados: 52
Autores: Souza,Maria Verônica de.
ABSTRACT: The problem considered in this review is related to the frequent use of corticosteroids (COs) (i.e. steroids) in the practice of equine medicine and surgery as a therapeutic strategy in the treatment of degenerative joint disease, commonly known as osteoarthritis (OA). This disease is one of the most common among the equine musculoskeletal diseases, and is clinically characterized by pain, lameness, joint effusion at the initial stage, which usually reduces with the progression of the disease, and reduced physical performance. Although steroids are considered excellent drugs in the control of clinical signs resulting from osteoarthritis, they also influence cellular activity through activation of various signaling mechanisms. However, they can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Degenerative joint disease; Steroids; Intra-articular drugs; Treatment.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100324

Transpiration and stomatal resistance variations of perennial tropical crops under soil water availability conditions and water deficit
Provedor de dados: 52
Autores: Sena,José Ozinaldo Alves de; Zaidan,Humberto Actis; Castro,Paulo Roberto de Camargo e.
During the dry and rainy seasons, determinations of stomatal resistance and transpiration of five tropical crops were carried out: guarana (Paullinia cupana Kunth), coffee (Coffea arabica L.), cashew (Anacardium occidentale L.), guava (Psidium guajava L.) and rubber (Hevea brasiliensis Muell. - Arg.) trees. Experimental design was done at randomized complete blocks with five replications. During the dry season there was a decrease in values of stomatal resistance in the following order: guarana &gt; coffee&gt; cashew&gt; guava &gt; rubber, with values from 2.5 to 30.0 s.cm-1. During the rainy season the stomatal resistance values varied from 1.5 to 3.0 s.cm-1. The guarana and coffee crops showed higher resistance to water transpiration when compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trees; Water relations; Tropical crops.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200007

Anatomy of pneumatophore of Mauritia vinifera mart
Provedor de dados: 52
Autores: Pereira,Luiz Alfredo Rodrigues; Calbo,Maria Elisa Ribeiro; Ferreira,Claiton Juvenir.
Pneumatophores of Mauritia vinifera Mart. were collected from six month-old plants maintained submerged in fresh water to induce pneumatophore formation. Twenty day-old pneumatophores had a quite prominent root cap. The epidermis was composed of hexagonal cells, tangentially distributed along the cylindric surface of the organ. In transverse section these pneumatophores had a simple epidermis over several layers of sclerified parenchyma, which covered an aerenchyma with large intercellular spaces. The endodermal cells had Casparian strips. The vascular cylinder was polyarch, with a pith and surrounded by a unisseriate pericycle. Anatomically the 4 month-old pneumatophores were similar to the younger ones, except for the absence of the epidermis. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buriti; Mauritia vinifera; Pneumatophores; Gas exchange.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300013

Alkaline phosphatase activity in plasma and liver of rats submitted to chronic exposure to fluoride
Provedor de dados: 52
Autores: Fernandes,Mileni da Silva; Iano,Flávia Godoy; Rocia,Vivian; Yanai,Marcela Mitsuko; Leite,Aline de Lima; Furlani,Tatiana Almeida; Buzalaf,Marília Afonso Rabelo; Oliveira,Rodrigo Cardoso de.
The aim of this study was to compare the effect of fluoride (F) on alkaline phosphatase activity in the liver and plasma of the rats. Four groups of male Wistar rats (n=6), which received drinking water containing 5, 15 or 50 ppm F or deionized water (control) throughout the experiment were included in the study. The animals were euthanized and had their tissues and blood plasma collected for the analysis of fluoride and alkaline phosphatase. There was an increase in F concentration in most tissues in the animals treated with higher F concentrations, except for the heart. The alkaline phosphatase assay showed an increase in the activity in the liver and blood plasma of the animals treated with fluoride concentrations of 15 and 50 ppm (p&lt;0.05). This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaline phosphatase; Fluoride; Liver; Kidney.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600014

Biodegradable foams based on starch, polyvinyl alcohol, chitosan and sugarcane fibers obtained by extrusion
Provedor de dados: 52
Autores: Debiagi,Flávia; Mali,Suzana; Grossmann,Maria Victória Eiras; Yamashita,Fábio.
Biodegradable foams made from cassava starch, polyvinyl alcohol (PVA), sugarcane bagasse fibers and chitosan were obtained by extrusion. The composites were prepared with formulations determined by a constrained ternary mixtures experimental design, using as variables: (X1) starch / PVA (100 - 70%), (X2) chitosan (0 - 2%) and (X3) fibers from sugar cane (0 - 28%). The effects of varying proportions of these three components on foam properties were studied, as well the relationship between their properties and foam microstructure. The addition of starch/PVA in high proportions increased the expansion index and mechanical resistance of studied foams. Fibers addition improved the expansion and mechanical properties of the foams. There was a trend of red and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava starch; Mechanical properties; Microstructure; Expansion.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500023

Indução in vitro de raízes adventícias em explantes de salix (Salix humboldtiana Willdenow)(Salix humboldtiana Willdenow)
Provedor de dados: 52
Autores: Paiva Neto,Vespasiano Borges de; Paiva,Renato; Furtado,Deborah E.
With the objective to investigate the in vitro behavior of salix (Salix humboldtiana Willdenow), leaf and nodal segments were inoculated in WPM medium supplemented with NAA and IBA. In leaf explants, the presence of these growth regulators, isolated or in combination, induced the formation of adventicious roots. Root hairs were only observed when NAA was present. In nodal explants, while the combination of 2,68 µM ANA + 2,46 µM IBA induced bud growth, the other treatments (4,92 µM IBA; 2,68µM ANA + 4,92 µM IBA; 5,37 µM ANA + 4,92 µM IBA; 5,37µM ANA) induced the formation of adventicious roots.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Salix; Adventicious roots; Root hairs.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100009

Screening of melon populations for resistance to Didymella bryoniae in greenhouse and plastic tunnel conditions
Provedor de dados: 52
Autores: Tsutsumi,Cláudio Yuji; Silva,Norberto da.
Studies were carried out to evaluate melon populations for resistance to Gummy Stem Blight, caused by Didymella bryoniae for use in breeding programs under greenhouse and plastic tunnel conditions. Were evaluated reactions of half-sib populations of PI 140471 and Anô nº 2 had as with susceptible check Eldorado 300. Plants were inoculated 45 days for needle prickling followed by application of 0.05 ml of 5.0 x 10(5) spores/ml suspension in axis of third and fourth true leaves.Resistance evaluation was made after 90 days of age using a scale varying one to five. PI 140471 and Anô nº 2 progenies were found superior and differed significantly with the checker Eldorado 300 for the F Test. Anô nº 2 possessing multiple resistance to diseases and desirable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucumis melo; Gummy stem blight; Plant breeding; Disease resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200002

Occurrence of intersexuality in " Lambaris" , Astyanax scabripinnis (Jenyns, 1842), small characids from the Brazilian streams
Provedor de dados: 52
Autores: Sá,Maria de Fátima Pereira de; Fragoso-Moura,Evelise Nunes; Fenerich-Verani,Nelsy; Ferro,Dagmar Aparecida de Marco.
During studies carried out with a small characid (Astyanax scabripinnis), from the Brazilian streams located in Cerrado biome, some gonads of fishes exhibited sparse oocytes in perinucleolar stage embedded in normally developing testicular tissues. Another pattern of gonad intersex with vitellogenic oocytes and mature lobules was observed in the gonads of fishes collected from a reservoir supplied by the Atlantic Forest stream. These gonads in different stages of maturation were examined histologically. The results revealed the patterns occurring in fishes of the same species, from four different populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intersexuality; Neotropical ichthyofauna; Characidae; Tetras; Stream fishes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200011

Release of potassium from rock powder by the yeast Torulaspora globosa
Provedor de dados: 52
Autores: Rosa-Magri,Márcia Maria; Avansini,Simoni Helena; Lopes-Assad,Maria Leonor; Tauk-Tornisielo,Sâmia Maria; Ceccato-Antonini,Sandra Regina.
The alteration of minerals in rocks and the availability of elements for plant nutrition require long periods of time, and microorganisms are thought to induce the release of potassium and phosphate from rocks. In this context, this work evaluates the role of the yeast Torulaspora globosa, isolated from the sugar cane rhizosphere, in the solubilization of potassium from alkaline ultramafic rock powder. The experiments were performed in liquid medium, with or without agitation, at 30°C with the following treatments: culture medium + alkaline ultramafic; culture medium + yeast suspension; and culture medium + yeast suspension + alkaline ultramafic. The results showed that as much as 38% of the total potassium in the rock was released in the medium with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosolubilization; Insoluble rocks; Alkaline ultramafic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400013

Sub-lethal Effects of Diazinon on Hematological Indices and Blood Biochemical Parameters in Indian Carp, Cirrhinus mrigala (Hamilton)
Provedor de dados: 52
Autores: Haider,Muhammad Jamal; Rauf,Abdul.
Indian carp (Cirrhinus mrigala) exposed to two sub-lethal concentrations (0.815 mg/L and 1.63 mg/L) of diazinon for 30 days showed a significant decrease in erythrocyte count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, plasma levels of total protein, albumin, globulin, activity of enzyme acetylcholinesterase and a significant increase in plasma glucose levels when compared with controlled fish (P&lt;0.05). Compared to the controlled fish, leukocyte count decreased significantly in fish exposed to both sub-lethal concentrations and the plasma activities of enzyme asparate aminotransferase and alanine aminotransferase increased significantly after 20 and 30 days in fish exposed to 0.815 mg/L of diazinon, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diazinon; Toxicity; Hematology; Biochemistry; Indian carp (Cirrhinus mrigala).
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600947

Economic Viability of Tomato Cultivation in Organic Farming System
Provedor de dados: 52
Autores: Santos Neto,José dos; Schwan-Estrada,Kátia Regina Freitas; Sena,José Ozinaldo Alves de; Telles,Tiago Santos.
ABSTRACT The aim of the present study is to evaluate the economic viability of tomato cultivation in a system of organic farming, and to compare it with the conventional farming system. The fixed and variable costs of both cultivation systems were calculated, as well as the costs of disease control with the application of alternative products and pesticides. The revenues were computed using commercial production and the direct sales price. The total cost per area was higher in the conventional system, while the cost per plant was greater in the organic system, since it used a lower plant population density. In the conventional system, 2.33 times more was spent on plant management, due to the fact that the number of sprayings was greater than that carried...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sustainability; Solanum lycopersicum; Cymbopogon citratus; Costs of production; Production systems.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100205

Parasitism capacity of Telenomus remus Nixon (Hymenoptera: Scelionidae) on Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) eggs
Provedor de dados: 52
Autores: Bueno,Regiane Cristina Oliveira de Freitas; Carneiro,Tatiana Rodrigues; Bueno,Adeney de Freitas; Pratissoli,Dirceu; Fernandes,Odair Aparecido; Vieira,Simone Silva.
This work studied the parasitism capacity of Telenomus remus Nixon (Hymenoptera: Scelionidae) on Spodoptera frugiperda (Smith) (Hymenoptera: Scelionidae) eggs at 15, 20, 25, 28, 31, and 35°C, aiming to use this natural enemy in biological control programs in crops where S. frugiperda was considered pest. The parasitism during the first 24 h was 60.90, 81.65, 121.05, 117.55 and 108.55 parasited eggs per female from egg masses of approximately 150 eggs, at 15, 20, 25, 28 and 31°C, respectively. Females of T. remus reached parasitism higher than 80% at 15, 20, 25, 28 and 31ºC at 5, 27, 8, 2, and 2 days, respectively. At 35ºC, there was no parasitism. The highest parasitism rates occurred at 20, 25, 28 and 31°C. T. remus female longevity varied from 15.7 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eggs parasitoid; Fall armyworm; Biological control; Integrated Pest Management.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100017

Chimeric ß-glucosidase between Cellvibrio gilvus and Ruminococcus albus enzymes
Provedor de dados: 52
Autores: Hoa,Truong Thi; Singh,Ajay; Iwakiri,Kenji; Aoyagi,Chika; Tokuyasu,Ken; Ohmiya,Kunio; Hayashi,Kiyoshi.
Based on homology search in the amino acid sequences, a chimeric ß-glucosidase with enzyme activity has been prepared by replacing C-terminal region of Thr509-Arg646 from Cellvibrio gilvus with N-terminal region of Arg160-Gly283 from Ruminococcus albus. The chimeric enzyme showed broader optimum pH and slightly higher heat stability compared with original C. gilvus enzyme.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chimeric; Cellvibrio gilvus; Ruminococcus albus; SS-glucosidase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300006

Anatomy of the underground system in Vernonia grandiflora Less. and V. brevifolia Less. (Asteraceae)
Provedor de dados: 52
Autores: Hayashi,Adriana Hissae; Appezzato-da-Glória,Beatriz.
This work dealt with the anatomy of the underground system in Vernonia grandiflora Less. and V. brevifolia Less. (Vernonieae; Asteraceae), two perennial geophytes, to elucidate their ability to sprout in the Brazilian Cerrado conditions. V. grandiflora, a subshrubby species, possessed a thickened underground system constituted by a xylopodium and many tuberous roots. The xylopodium had stem and root structure and its buds were axillary or originated from the cortical parenchyma proliferation. The tuberous roots produced by this organ were adventitious and accumulated inulin-type fructans mainly in the cortical parenchyma. The thickened underground system of V. brevifolia, an herbaceous species, was a tuberous primary root whose buds originated from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bud; Tuberous root; Xylopodium; Fructan; Cerrado.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700009

Macro and microscopic characters of the aerial vegetative organs of Carqueja: Baccharis usterii Heering
Provedor de dados: 52
Autores: Budel,Jane Manfron; Duarte,Márcia do Rocio.
This work aimed to study the macro and microscopic characters of the aerial vegetative organs of Baccharis usterii for quality control purposes. The plant was prepared for light and scanning electron microscopic analyses. The stem consisted of three-winged cladodes with small leaves. In the caulinar axis, these was a uniseriate epidermis, chlorenchyma alternating with angular collenchyma, perivascular fibre caps adjoining the phloem which was outside the xylem, and parenchymatic pith. The wings and the leaves were alike, presenting a uniseriate epidermis, palisade parenchyma next to the both sides of the epidermis and spongy parenchyma traversed by minor collateral vascular bundles in the central region. In the leaf midrib, a single collateral vascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baccharis usterii; Carqueja; Medicinal plant; Morpho-anatomy; Secretory ducts.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100016

Antibiosis and dark-pigments secretion by the phytopathogenic and environmental fungal species after interaction in vitro with a Bacillus subtilis isolate
Provedor de dados: 52
Autores: Machado,Alexandre Paulo; Vivi,Viviane Karolina; Tavares,José Roberto; Gueiros Filho,Frederico José; Fischman,Olga.
In this work, different reactions in vitro between an environmental bacterial isolate and fungal species were related. The Gram-positive bacteria had terminal and subterminal endospores, presented metabolic characteristics of mesophilic and acidophilic growth, halotolerance, positive to nitrate reduction and enzyme production, as caseinase and catalase. The analysis of partial sequences containing 400 to 700 bases of the 16S ribosomal RNA gene showed identity with the genus Bacillus. However, its identity as B. subtilis was confirmed after analyses of the rpoB, gyrA, and 16S rRNA near-full-length sequences. Strong inhibitory activity of environmental microorganisms, such as Penicillium sp, Aspergillus flavus, A. niger, and phytopathogens, such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus subtilis; Phytopathogenic fungi; Environmental fungi; Microbial interaction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500001

Recent developments in microbial oils production: a possible alternative to vegetable oils for biodiesel without competition with human food?
Provedor de dados: 52
Autores: Christophe,Gwendoline; Kumar,Vinod; Nouaille,Régis; Gaudet,Geneviève; Fontanille,Pierre; Pandey,Ashok; Soccol,Carlos Ricardo; Larroche,Christian.
Since centuries vegetable oils are consumed as human food but it also finds applications in biodiesel production which is attracting more attention. But due to being in competition with food it could not be sustainable and leads the need to search for alternative. Nowdays microbes-derived oils (single cell oils) seem to be alternatives for biodiesel production due to their similar composition to that of vegetable oils. However, the cold flow properties of the biodiesel produced from microbial oils are unacceptable and have to be modified by an efficient transesterification. Glycerol which is by product of transesterification can be valorised into some more useful products so that it can also be utilised along with biodiesel to simplify the downstream...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Oleaginous microorganisms; Biodiesel; Vegetable oil; Lipid; Transesterification.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100004

Microalgae: Cultivation Aspects and Bioactive Compounds
Provedor de dados: 52
Autores: Coêlho,Diego de Freitas; Tundisi,Louise Lacalendola; Cerqueira,Keilla Santos; Rodrigues,Jacqueline Rego da Silva; Mazzola,Priscila Gava; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
Abstract Microalgae are aquatic unicellular microorganisms that can be found both in freshwater and marine systems; are capable of photosynthesis; and can grow as individual cells or associated in chains or small colonies. Microalgae cultivation has gained large momentum among researchers in the past decades due to their ability to produce value metabolites, remarkable photosynthetic efficiency, and versatile nature. The wide technological potential, and thus increasing amount of scattered knowledge, may become the very first barrier that a post graduating student, or any non-specialist reader, will face when introduced to the subject. In this review paper, we access the core aspects of microalgae technology, covering their main characteristics, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalgae; Microalgae Selection and Isolation; Green Products; Microalgae Cultivation; Bioactive Compounds.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000101000

Technical and economic viability of the installation of a hybrid solar-wind generation system in a Brazilian industry
Provedor de dados: 52
Autores: Lara Filho,Mauro Obladen de; Unsihuay-Vila,Clodomiro; Silva,Vilson Roiz Gonçalves Rebelo da.
Abstract The demand for electricity is growing worldwide. At the same time, the non-renewable natural resources that account for a large proportion of the global energy matrix are rapidly depleting, which will pose a major challenge in the near future. Therefore, micro-grid models that use renewable energy sources, such as solar and wind, are rapidly developing and are becoming economically viable alternatives. The objective of this study was to evaluate the economic viability of installing solar and wind power generation systems in the NOVVALIGHT electrical components factory located in Campo Largo, Paraná, Brazil. The most viable model was the combination of solar and wind energy, which would generate approximately 260 MWh of energy per year. Using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distributed generation; Solar energy; Wind energy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000200204

Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum
Provedor de dados: 52
Autores: Gasparotto,Elainy Patrícia Lino; Abrão,Sorrailla Carolina Carrard; Inagaki,Suely Yaeko; Tessmann,Dauri José; Kemmelmeier,Carlos; Tessmann,Ione Parra Barbosa.
A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galactose oxidase; Fusarium graminearum; Enzyme.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500004

Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils
Provedor de dados: 52
Autores: Mariano,Adriano Pinto; Angelis,Dejanira de Franceschi de; Pirôllo,Maria Paula Santos; Contiero,Jonas; Bonotto,Daniel Marcos.
This work investigated the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils collected at three service stations. Batch biodegradation experiments were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO2 production. Biodegradation efficiency was also measured by quantifying the concentration of hydrocarbons. In addition to the biodegradation experiments, the capability of the studied cultures and the native microorganisms to biodegrade the diesel oil purchased from a local service station, was verified using a technique based on the redox indicator 2,6 -dichlorophenol indophenol (DCPIP). Results obtained with this test showed that the inocula used in the biodegradation experiments were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioremediation; Bioaugmentation; Soil; Diesel oil; Service station.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500030

Identification of yeasts isolated from the pulp in nature and the production of homemade "umbu" wine
Provedor de dados: 52
Autores: Melo,Dângelly Lins Figuerôa Martins de; Santos,Flávia Cristiana; Barbosa Junior,Antônio Marcio; Santos,Patrícia Oliveira; Carnelossi,Marcelo Augusto Gutiérrez; Trindade,Rita de Cássia.
The goal of this work was to select strains of fermentation yeasts and test them in a small-scale production of " umbu" fruit wine. The fruits were collected in N. Sra da Glória city in Sergipe, Brazil. The fruit pulp was diluted and aliquots were inoculated on YMA plates. The yeasts strains were submitted to fermentation using "umbu" fruit juice as substrate, exception with to those strains of species related to pathological processes. The yeasts tested produced a beverage characterized between dry to light due to its alcoholic content with very nice taste, translucent appearance, of green to light yellow color.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spondias tuberosa; Wine of fruit; Non-saccharomyces.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500017

Infestation of Mangroves by the Invasive Moth Hyblaea puera (Cramer, 1777)(Lepidoptera: Hyblaeidae)
Provedor de dados: 52
Autores: Faraco,Luiz Francisco Ditzel; Ghisi,Conrado Locks; Marins,Marina; Ota,Sueli; Schühli,Guilherme Schnell.
Abstract We report the first known infestation of mangroves by the invasive moth Hyblaea puera in Paraná, Southern Brazil. The infestation caused massive defoliation of Avicennia schaueriana trees, affecting approximately 20,000 hectares of mangroves. We discuss the implications for conservation and management, focusing on protected areas, the ecology of mangroves, and local livelihoods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pest quarantine; Ecosystem services; Insect-plant interactions; Leaf consumption; Litterfall.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100409

Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation.
Provedor de dados: 52
Autores: Ray,Syandan Sinha; Ali,Nasim.
ABSTRACT Multipurpose use of bamboo in rural life makes it as poor man’s timber in Asian countries. Deforestation and industrialization leads to destruction of natural forest. To replenish, a rapid plantation of bamboo could be one of the possible solutions. Bamboo is propagated mainly by vegetative methods though it is not suitable for large scale plantation because of several limitations. Micropropagation is gaining importance for large scale propagation because of its capability in raising huge number of true to type propagules in a limited space in very short span of time. Like any other plant, the chief constraint of bamboo micropropagtion is in vitro contamination arises from several sources including explants. Most of the contaminants are reduced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotic contamination; Sterilization; Bamboo; Micropropagation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100207

Hepatoprotective Effect of Quercetin Pretreatment Against Paracetamol-Induced Liver Damage and Partial Hepatectomy in Rats
Provedor de dados: 52
Autores: Barros,Pedro Paulo; Silva,Gustavo Henrique da; Gonçalves,Gisele Mara Silva; Oliveira,Jessica Cristiane; Pagnan,Livia Gonçalves; Arco-e-Flexa,Luiza.
ABSTRACT Quercetin has potent antioxidant action and a hepatoprotective role. The aim of this study was to evaluate the hepatoprotective action of quercetin pretreatment in paracetamol-induced liver damage (PILD) and structural injury resulting from partial hepatectomy (PH). In the first model, Wistar rats received oral quercetin (50mg/kg/day) during 8 days. On the 8th day, 3g/kg paracetamol were added. In the second model, the same quercetin dose was given during 7 days and rats were submitted to PH on the 8th day. Blood samples were obtained for determination of enzyme levels. Liver, heart, kidney and lung tissue were also collected for assessment of quercetin biodistribution and/or histological analyses. The results obtained after PILD were more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quercetin; Paracetamol; Partial hepatectomy; ALT; AST.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100302

Leaf Morphological Plasticity of Tree Species from Two Developmental Stages in Araucaria Forest
Provedor de dados: 52
Autores: Vieira,Willyam de Lima; Boeger,Maria Regina Torres; Cosmo,Nelson Luiz; Coan,Alessandra Ike.
This study compared the morphological and anatomical variations of the leaves of four shade-tolerant tree species Allophylus edulis (St.-Hil.) Radlk (Sapindaceae), Casearia sylvestris Sw. (Salicaceae), Cupania vernalis Cambess. (Sapindaceae) and Luehea divaricata Mart. (Malvaceae) from a fragment of Araucaria forest in two developmental stages. Morphological and anatomical traits, such as leaf and tissue thickness, leaf area, leaf dry mass, specific leaf area, leaf density and stomata density were measured from 30 leaves of each developmental stage. The phenotypic plasticity index was also calculated for each quantitative trait. The results showed that the four species presented higher mean values ​​for specific leaf area and spongy/palisade parenchyma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf anatomy; Leaf morphology; Ombrophyllous Mist Forest; Shade tolerance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400476

Dynamic of decapod crustacean larvae on the entrance of Guanabara bay
Provedor de dados: 52
Autores: Fernandes,Lohengrin Dias de Almeida; Bonecker,Sérgio Luiz Costa; Valentin,Jean Louis.
In the present study, we observed seasonal variations in the density of decapod larvae as well as changes in density related to diurnal and tidal cycles. Among the decapod larvae collected, portunids and grapsids were the most abundant, especially during nocturnal ebb tides and near the surface. The same results were obtained in late winter (September) and late summer (March). These results demonstrated a flow of decapod larvae from Guanabara Bay to adjacent coastal waters. Luciferid (Lucifer faxoni) was the only group with high densities during flood tides and we suggest this is an evidence of L. faxoni larvae entering Guanabara Bay in late winter. Probably these changes in distribution of Lucifer faxoni among winter and summer were related to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea Decapoda larvae; Zooplankton; Vertical Distribution; Vertical Migration; Dynamic; Guanabara Bay.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600013

Effect of Intermittent Feeding on Gonadal Function in Male And Female NMRI Mice During Chronic Stress
Provedor de dados: 52
Autores: Ehteram,Badri Zarrin; Sahraei,Hedayat; Meftahi,Gholam Hossein; Khosravi,Maryam.
ABSTRACT Stress can inhibit gonadal activity via Hypothalamus-Pituitary-Gonad (HPG) axis activity suppression. In the present study, effects of intermittent feeding (IF) on gonadal function under stress in male and female mice were evaluated. Twenty eight male and twenty eight female mice's were divided into four groups. The control group received adequate food and water without stress. The second group received four days of electric shock without food deprivation. The third group was deprived of food two hours/day for a week, and the fourth group was deprived of food (2 hours/day for seven consecutive days) and then electric foot shock stress was applied to them for four days. Blood samples were collected from all animals for plasma testosterone, estrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogen; Gonad; IL-6; Intermittent Feeding; Stress; Testosterone.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100439

Identification and colonization of endophytic fungi from soybean (Glycine max (L.) Merril) under different environmental conditions
Provedor de dados: 52
Autores: Pimentel,Ida Chapaval; Glienke-Blanco,Chirlei; Gabardo,Juarez; Stuart,Rodrigo Makowiecky; Azevedo,João Lúcio.
A total of 297 endophytic fungi were isolated from 1728 leaf and stem fragments collected about twenty and forty days after germination from soybean (Glycine max (L.) Merril) plants grown in the field and a greenhouse. The fungi belonged to eight groups, six dematiaceous genera (Alternaria, Cladosporium, Chaetomium, Curvularia, Drechslera and Scopulariopsis) and the non-dematiaceous genera Acremonium, Aspergillus, Colletotrichum, Fusarium, Paecilomyces and Penicillium along with some Mycelia sterilia.. There were qualitative and quantitative differences in the type and number of isolates obtained from greenhouse and field-grown plants, with more isolates being obtained from the latter. No difference was found in the number of fungi isolated from leaves and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic fungi; Soybean; Biological control.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600003

Physiological Characterization of Fungal Inoculum for Biotechnological Remediation of Soils
Provedor de dados: 52
Autores: Ballaminut,Nara; Machado,Kátia Maria Gomes; Oliveira,Luisa Helena dos Santos; Matheus,Dácio Roberto.
The aim of this work was to study the bioremediating potential of Lentinus crinitus CCIBt2611 according to the physiological condition of the inoculum. Inoculum was prepared using sugarcane ground husk (C:N 90), at several physiological ages and applied in soil contaminated with pentachlorophenol. The inoculum's potential was assessed by evaluating the mycelium's vigor at soil's colonization, determination of peroxidase and phenoloxidase activities, in vitro degradation of Remazol Brilliant Blue R and in vivo degradation of pentachlorophenol. The results showed that the assessed parameters were relevant to identify the quality of the inoculum. For L. crinitus, 10 day old inoculum showed good soil-colonization speed with significant enzymatic activities,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laccase; Manganese-dependent peroxidase; Organochlorine degradation; Fungal inoculum quality; Bioremediation; Environmental sanitization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400561

Differential expression of Zymomonas mobilis sucrase genes (sacB and sacC) in Escherichia coli and sucrase mutants of Zymomonas mobilis
Provedor de dados: 52
Autores: Gurunathan,Sangiliyandi; Gunasekaran,Paramasamy.
The sacB and sacC genes encoding levansucrase and extracellular sucrase respectively were independently subcloned in pBluescript (high copy number) and in Z. mobilis-E. coli shuttle vector, pZA22 (low copy number). The expression of these genes were compared under identical background of E. coli and Z. mobilis host. The level of sacB gene expression in E. coli was almost ten fold less than the expression of sacC gene, irrespective of the growth medium or the host strain. In Z. mobilis the expression of sacB and sacC genes was shown to be subject to carbon source dependent regulation. The transcript of sacB and sacC was three fold higher in cells grown on sucrose than in cells grown on glucose/fructose. Northern blot analysis revealed that the transcript...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymomonas mobilis; Escherichia coli; Expression; Levansucrase; Extracellular sucrase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300001

Biomonitoring of Heavy Metals (Fe, Zn, Cu, Mn, Cd and Cr) in Oysters: Crassostrea rhizophorae of Mangrove Areas of Alagoas (Brazil)
Provedor de dados: 52
Autores: Azevedo,Joaquim Alexandre Moreira; Barros,Alexandre Bomfim; Miranda,Paulo Rogério Barbosa de; Costa,João Gomes da; Nascimento,Velber Xavier.
Abstract Mangroves are tropical and subtropical flooded forests that generally develop in estuarine areas over unstable sediments protected from the action of waves in the intertidal zone with an environment characterized by a great diversity of fauna and flora. Thus, the present study aimed to evaluate the levels of heavy metals that are absorbed by mangrove oysters, in estuarine systems in the Alagoas coast by determining the concentrations of Fe, Cu, Mn, Zn, Cd and Cr. Two areas, which consisted of both a collection during the rainy season and the dry season, were selected for sampling of mangrove oysters. In each collection, seven samples were collected, at seven different points, where each sample contained four oysters. In the laboratory the oysters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangrove; Mangrove Oyster; Heavy Metals.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100415

Flight range extension in Polistes simillimus Zikán, 1951 (Hymenoptera, Vespidae)
Provedor de dados: 52
Autores: Prezoto,Fábio; Gobbi,Nivar.
The aim of this study was to determine the flight range extension of the social wasps, Polistes simillimus. The results of the 125 wasps-carried out tests originating from 10 colonies in post-emergency stage demonstrated that the flight range extension of P. simillimus was of approximately 150m, resulting in a collection area of about 70.650m² by colony. It suggested wasp's efficiency as a biological control agent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Social wasps; Polistes simillimus; Flight range extension; Nest return.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800011

Diet of Iheringichthys labrosus (Pimelodidae, Siluriformes) in the Itaipu Reservoir, Paraná River, Brazil-Paraguay
Provedor de dados: 52
Autores: Abes,Sara da Silva; Agostinho,Angelo Antonio; Okada,Edson Kiyoshi; Gomes,Luiz Carlos.
Iheringichthys labrosus is a secondary commercial fish species in the Itaipu Reservoir, but it is important in other reservoirs of the basin. However, little information exists about this species in the literature. The purpose of this study was to describe the diet of this species in the Itaipu Reservoir. Stomachs of 306 fishes were analyzed, collected from October 1994 to September 1996 in the differents zones of the reservoir (according to a longitudinal gradient). The reservoir was separated into riverine (upper reach), transitional (middle reach), and lacustrine (next to the dam) zones. Main items in the diet of I. labrosus were aquatic insects (mainly Chironomidae) and mollusks (mainly Bivalvia). Their presence in the diet did not vary according to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Itaipu Reservoir; Freshwater; Fish; Feeding; Diet composition; Benthophagous.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100014

Improvement of biotechnological xylitol production by glucose during cultive of Candida guilliermondii in sugarcane bagasse hydrolysate
Provedor de dados: 52
Autores: Silva,Débora Danielle Virgínio da; Mancilha,Ismael Maciel de; Silva,Silvio Silvério da; Felipe,Maria das Graças de Almeida.
The effect of glucose on xylose-to-xylitol bioconversion by Candida guilliermondii was examined by adding it to sugarcane bagasse hydrolysate medium to obtain different glucose:xylose ratios (1:25, 1:12, 1:5 and 1:2.5). Under experimental conditions, increasing glucose:xylose ratio improved the assimilation of the xylose present in the hydrolysate by yeast, resulting in biomass increase, and in the formation of xylitol and glycerol/ethanol by-products. Maximum values of xylitol yield (0.59 g g-1) and volumetric productivity (0.53 g l-1.h-1) were obtained with glucose:xylose ratio of 1:5, resulting in the higher conversion efficiency (64.3%).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida guilliermondii; Glucose; Sugarcane bagasse hydrolysate; Xylitol; Xylose.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200005

L-arginine metabolism in mitochondria isolated from the liver of Antarctic fish Notothenia rossii and Notothenia neglecta
Provedor de dados: 52
Autores: Rodrigues,Edson; Ribeiro,Ana Cristina Miguez Teixeira; Bacila,Metry.
The arginase tissue distribution, the biochemical properties of the argininolytic system and the subcellular localization of the enzymes carbamoylphosphate synthetase, ornithinecarbamoyl transferase, glutamine synthetase and arginase in Antarctic fish, N. neglecta and N. rossii were the main aims of the present work. The tissue with highest argininolytic activity was the kidney distal portion amounting as much as four times the specific activity of the hepatic tissue. Arginase and ornithine carbamoyltransferase were found as mitochondrial enzymes, while glutamine synthetase and carbamoylphosphate synthetase were found as cytosolic enzymes. Argininolytic assays with isolated mitochondria gave values of Kmapp for the hydrolysis of arginine 2 to 3.5 times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antarctic fish; Arginine; Arginase; Urea cycle; N. neglecta; N. rossii.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600017

Influence of a native strain of Staphylococcus xylosus on the microbiological, physicochemical and sensorial characteristics on milano salami type
Provedor de dados: 52
Autores: Fiorentini,Ângela Maria; Sawitzki,Maristela Cortez; Bertol,Teresinha Marisa; Cunha Júnior,Anildo; Sant'Anna,Ernani Sebastião.
In this work, the influence of native starter cultures on the microbiological, physicochemical and sensorial characteristics of Milano salami type was studied. Two batches of Milano salami type were produced: Batch A, with the addition of Staphylococcus xylosus U5 and Batch B (control) without the starter culture. The Milano salami type was characterized by an important microbial activity of coagulase-negative staphylococci (CNS) that resulted in substantial growth in Batch A during the ripening with an initial count of 7.60 log cfu.g-1 and reached 9.84 log CFU.g-1 after 14 days. Bacterial enzymes that showed efficient activity under the conditions found in Milano salami type were catalase, nitrite and nitrate reductase, contributing for sensory and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus xylosus; Starter cultures; Milano salami type.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400027

Possible Associations between Bovine Growth Hormone Gene Polymorphism and Reproductive Traits
Provedor de dados: 52
Autores: Unanian,Maria Marina; Barreto,Cristine Chaves; Cordeiro,Célia Maria Torres; Freitas,Alfredo Ribeiro; Josahkian,Luiz Antonio.
The polymorphism of the bovine growth hormone gene (bGH) was studied in 211 Nellore pure breed males for association with reproductive traits. Scrotal circumference and testosterone concentrations were collected monthly from 10 until 16 months of age. Additionally, testicular growth rates were calculated. DNA was amplified by PCR and digested using Msp I and Hae III restriction enzymes. Every polymorphism presented two alleles. The predominant alleles were D (0.85) and F (0.98), respectively, and genotype EE - bGH/Hae III was missing. Significant association (P&lt;0.05) between bGH/Msp I polymorphism, scrotal circumference and testicular growth after puberty, as well as between bGH/Hae III and testosterone concentrations at puberty were detected. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bull; Growth hormone gene polymorphism; Testosterone concentrations.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300007

Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124)
Provedor de dados: 52
Autores: Mendonça,Analucia Longman; Mariano,Rosa de Lima Ramos; Araújo,Janete Magali de; Cavalcante,Uided Maaze T.
Forty-four Bacillus spp. strains obtained from sugar cane derivates and residues, six of them isolated in this work, were tested using Tween 80 as substrate (agar-Tween 80 medium), in order to determine their esterase activity through the enzymatic index averages. After statistic analysis, B. cereus (C124) strain, which presented better results, was submitted to genetic improvement by treatment with ultraviolet light (UV). The survival curve pointed out 28" as the time necessary to obtain 30% of survivors. Fifty survivors and the wild strain C124 were compared in relation to their esterase activity as mentioned previously. The wild strain and the mutant C124UV35, which showed enzymatic index average higher than C124, were characterized in polyacrilamide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Genetic improvement; Bacillus spp.; B. cereus; Esterase.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100011

Use of smoking to add value to the salmoned trout
Provedor de dados: 52
Autores: Salán,Eduardo Oliveira; Galvão,Juliana Antunes; Oetterer,Marília.
The objective of this study was to make a comparison between the characteristics of salmoned trout (Salmo truta) and salmon (Salmo salar) in terms of coloration (CIE L*a*b*), proximate composition, pH and sensory attributes of fresh and smoked fillets in order to obtain a possible alternative for the substitution of imported salmon for Brazilian salmoned trout as a commercial equivalent for retail sales. Results showed that no statistical difference was found for most of the sensory attributes analyzed and for pH. Although the coloration of the fillets presented a statistical difference, the trout presented a more intense coloration than the salmon, proving to be, therefore, convenient to agroindustry.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish conservation; Salmoned trout; Salmo salar; Agroindustry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100007

Biochar and Trichoderma harzianum for the Control of Macrophomina phaseolina
Provedor de dados: 52
Autores: Araujo,Alyson Silva de; Blum,Luiz Eduardo Bassay; Figueiredo,Cícero Célio de.
Abstract This study is based on the importance of biological control methods and the lack of information on the effect of biochar (BCH) from sewage sludge associated or not with Trichoderma harzianum on the control of Macrophomina phaseolina in the bean crop (Phaseolus vulgaris, cv. BRS Estilo). Biochar from sewage sludge, pyrolyzed at 500 ºC and used in low concentration (0.5%), has a direct effect on the in vitro control of M. phaseolina. However, higher BCH concentrations stimulated the growth of the pathogen. In culture medium with or without BCH, T. harzianum (strain 1306) inhibited the mycelial growth of M. phaseolina. The addition of BCH + T. harzianum reduced the deleterious effects caused by M. phaseolina on bean plants. This study demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrolysis; Biological control; Gray rot.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100202

Biotreatment effects in films and blends of PVC/PCL previously treated with heat
Provedor de dados: 52
Autores: Campos,Adriana de; Franchetti,Sandra Mara Martins.
Studies were carried out on biomodification and the pre-heat (130(0)C) influence on Poly (vinyl chloride) / Poly (epsilon-caprolactone) films. The results showed that heat pre-treatment improved the biomodification of PVC films, but it inhibited the biomodification of the PVC/PCL film.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotreatment; Thermal treatment; UV-Visible; PVC/PCL Blend.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200010

Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
Provedor de dados: 52
Autores: Terra,Danielle Amorim; Amorim,Lúcia de Fátima; Catanho,Maria Tereza Jansen de Almeida; Fonseca,Adenilson de Souza da; Santos-Filho,Sebastião David; Brandão-Neto,José; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario.
The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p&lt;0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemisia vulgaris L.; Technetium-99m; Blood constituents; Labeling.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600015

Morphological and physiological responses of Cedrela Fissilis Vellozo (Meliaceae) seedlings to light
Provedor de dados: 52
Autores: Santos,Débora Leonardo dos; Rakocevic,Miroslava; Takaki,Massanori; Ribaski,Jorge.
Seeds of Cedrela fissilis Vellozo were planted and maintained under two distinct conditions: at east border of a forest with red: far-red ratio of 1.15 and under canopy with photosynthetic photon flux density of 0.22-7% of full sun radiation and red: far-red ratio of 0.21-0.36. Seedling growth (height and stem diameter) was faster under sun, the development of roots more continuous and the number of leaves almost twice of that of shade plants. The leaf area was 10 times greater in sun plants with 15-25 leaflets per leaf while under shade only 5 to 10 leaflets were found per leaf. In shade plants, a higher proportion of dry mass was found in aerial parts. Leaves of sun plants had the capacity of gas exchange to respond to high light radiation, but leaves...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photosynthesis; Seedling morphogenesis; Shade.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100020

Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
Provedor de dados: 52
Autores: Santos,Neyliane Gonçalves dos; Amaral,Ademir; Cavalcanti,Mariana Brayner; Neves,Maria Amélia Batista; Machado,Cíntia Gonsalves de Faria.
The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Combined therapy; Radiation effect; P-glycoprotein; Multidrug resistance; Functional analysis; Flow cytometry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018

Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae
Provedor de dados: 52
Autores: Malvessi,Eloane; Silveira,Mauricio Moura da.
A liquid medium containing wheat bran, salts and a source of inducer (pectin) was found to be suitable for the production of exo- and endo-polygalacturonases by Aspergillus oryzae CCT3940. Induction of polygalacturonases by purified pectin was significantly higher than when rinds of citrus fruits were used as inducer. A. oryzae growth was favoured by pH close to 4, although a drop of pH to around 3 was needed for enzymes production. Afterwards, decreasing activities were observed with the normal increase in pH to near neutrality. The highest activities were achieved with an initial pH of 4 and controlled when it decreased to a value slightly below 3 (159 units endo-PG.mL-1 at 83 h and 45 units exo-PG.mL-1 at 64 h), being the loss in polygalacturonases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinase; Polygalacturonase; Submerged process; Production medium; PH.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500004

Growth of Crotalaria juncea L. supplied with mineral nitrogen
Provedor de dados: 52
Autores: Mendonça,Elenira Henrique Miranda; Schiavinato,Marlene Aparecida.
Plants of Crotalaria juncea inoculated with Rhizobium were treated with nutrient solution containing 10 or 20mg of either N/NO3 or N/NH4.plant-1.week-1 . The control plants received nutrient solution without N. An investigation was conducted on the effect of these sources of N on growth and nitrogen fixation of plants with 30, 60 and 90 days after sowing (DAS). Those that received mineral N presented higher growth than -N plants, but the presence of nodules occurred in all the treatments. Plants treated with NH4 presented higher N content until 60 days. The highest concentrations of leghemoglobin and protein in nodules were found at 30 DAS and there was no difference in leghemoglobin content between treatments for any age and in protein from 60 DAS....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonium; Nitrate; Rhizobium; Nitrogen fixation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200003

Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels
Provedor de dados: 52
Autores: Carvalho,Dayse Cristina de; Silva,André Luís Lopes da; Schuck,Mariane Ruzza; Purcino,Marivel; Tanno,Guilherme Nakao; Biasi,Luiz Antonio.
The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM) in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arginine; Nodal segment; Glucose; Adenine; Glycine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200004

Water balance in karst: case study of the Ribeirão da Onça catchment in Colombo City, Paraná State - Brazil
Provedor de dados: 52
Autores: Vestena,Leandro Redin; Kobiyama,Masato.
With the objective to analyze the hydrological processes and the water balance, a simplified water balance method and the modified Penman formula were applied to the Ribeirão da Onça river catchment in Colombo city, Paraná State. For the analysis, the data obtained during the monitoring period from 1997 to 2000 were utilized. The results showed that the values of the real evapotranspiration estimated by the method of the simplified water balance were negative for the certain periods and for other period sometimes more than those of the potential evapotranspiration estimated by the modified Penman formula. It implied the existence of significant groundwater recharge from other catchments to the study area, and indicates that the simplified water balance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water balance; Evapotranspiration; Karst.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500020

Fumonisins B1 and B2 in corn-based products commercialized in the state of Santa Catarina - Southern Brazil
Provedor de dados: 52
Autores: Scaff,Rejane Maria Cirra; Scussel,Vildes Maria.
Corn flour, "canjica" (corn grits), corn flakes and popcorn for human consumption, commercialized in Santa Catarina (n=82), were analyzed in order to detect the presence of fumonisins B1 (FB1) and B2 (FB2). From the samples, 92.68% showed detectable levels of Fumonisins (FBs). Corn flour showed the highest level of contamination (91.5%) with average levels of 3.811 and 5.737 mg/g for the home-processed and industrialized products, respectively. The next most contaminated product was popcorn with a average of 2.872 mg/g and an occurrence in 91.6% of the samples. All samples of corn flakes were contaminated with an average of 1.307 mg/g. The product with the lowest levels of FBs was "canjica" with a average contamination of 0.732 mg/g. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn; Fumonisins; Brazilian corn products.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600011

Development of mint (Mentha piperita L.) grown on biosolids: evaluation of productivity and essential oil content
Provedor de dados: 52
Autores: Scavroni,Joseane; Ferreira,Leonardo Cesar; Valmorbida,Janice; Boaro,Carmen Sílvia Fernandes.
Development of Mentha piperita L . on biosolids amended soil with levels equivalent to 0, 28, 56 and 112 t ha-1 was evaluated. In order to measure the productivity and its relation with mint essential oil yield, different indices were determined: leaf area, total and several organ dry matter, leaf area ratio, specific leaf area, net assimilation rate and relative growth rate at 30, 44, 58, 72 and 86 days after planting (DAP), and essential oil yield at 90, 110 and 120 DAP. Physiological indices revealed that biosolids prolonged the vegetative phase of the plants, which adapted themselves to the presence of biosolids with time. Plants showed inverse behaviors in relation to productivity, resulting from the primary metabolism, represented by the shoot dry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth analysis; Peppermint; Essential oil; Sewage-sludge; Leaf area; Dry matter.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200014

Blood parameter analysis and morphological alterations as biomarkers on the health of Hoplias malabaricus and Geophagus brasiliensis
Provedor de dados: 52
Autores: Romão,Silvia; Donatti,Lucélia; Freitas,Matheus O.; Teixeira,Josiane; Kusma,Josiana.
This study aimed to assess the influence of the environment on fish health. Samples of Hoplias malabaricus and Geophagus brasiliensis, were collected from three different environments: area I was urban and areas II and III were rural. Analyses of red blood cell count, microhematocrit, hemoglobin concentration, white blood cell count and differential white cell count in blood smear were carried out. Mean corpuscular volume and mean corpuscular hemoglobin concentration were calculated. To analyze morphological alterations, gills, liver, kidney and gonads were submitted to routine histological processing. Individuals collected from area III had slightly lower blood indices than collected from area I . Severe kidney changes, degeneration of and crystallization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Bioindicators; Biomarkers; Morphological alterations; Hematology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400012

Dose absorbed by technologists in positron emission tomography procedures with FDG
Provedor de dados: 52
Autores: Amaral,Ademir; Itié,Christian; Bok,Bernard.
The objective of this work was to evaluate radiation doses delivered to technologists engaged in different tasks involving positron emission tomography (PET) studies with FDG (fluorodeoxyglucose). This investigation was performed in two French nuclear medicine departments, which presented significant differences in their arrangements and radiation safety conditions. Both centers administered about 300 MBq per PET/CT study, although only one of them is a dedicated clinical PET center. Dose equivalent Hp(10) and skin dose Hp(0.07) were measured using Siemens electronic personnel dosimeters. For assessment dose absorbed by hands during drawing up of tracer and injection into the patient, a Polimaster wristwatch gamma dosimeter was employed. Absorbed dose and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electronic Dosimeters; Radioprotection; Technologists; PET.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600016

Power Flow Analysis and Self-recovery of Electrical Energy Distribution Network Using Artificial Neural Networks
Provedor de dados: 52
Autores: Avelar,Fabio da Silva; Fritzen,Paulo Cícero; Furucho,Mariana Antônia Aguiar.
ABSTRACT A computational model for self-recovery of electricity distribution network was developed to simulate it, emulated by the IEEE 123 node model. The electrical system considered has automatic switches capable of identifying a momentary failure in the line and finding the best reconfiguration for its reclosing. An artificial neural network (ANN), backpropagation, was used to classify the type of failure and determine the best reconfiguration of the distribution network. Initially, five power failure scenarios were simulated in certain different parts of the power grid, and power flow analysis via OpenDSS was performed. Next, the most suitable switching was observed within the shortest time interval to restore the power supply. With the purpose of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distribution Networks; Optimization; Self-recovery of networks; Smart Grid.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200223

Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants
Provedor de dados: 52
Autores: Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Oliveira,Rosângela Claret de; Turilli,Carlo; Lunardi,Michele; Otonel,Rodrigo Alejandro Arellano; Freitas,Julio Cesar de.
Chlamydophila abortus (C. abortus) infection is related to reproductive failure in domestic ruminants. Although it has not been well characterized worldwide, this pathogen has already been identified in some European countries and in the USA. In Brazil, preliminary studies have shown serological evidence of C. abortus infection in herds with low antibody prevalence. Until now, the identification of C. abortus in biological samples from females presenting reproductive failures has not been described in Brazilian herds of domestic ruminants. The aim of this study was to evaluate the presence of the C. abortus in a collection of abortions from cattle (n=85), sheep (n=12), and goats (n=8), in samples of vaginal mucus from cows (n=13), sheep (n=90), and goats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Small ruminants; Chlamydophila abortus; Abortion; Vaginal mucus; Semen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700015

Conjoint effect of oil-seed cakes and Pseudomonas fluorescens on the growth of chickpea in relation to the management of plant-parasitic nematodes
Provedor de dados: 52
Autores: Rizvi,Rose; Mahmood,Irshad; Tiyagi,Sartaj A; Khan,Zehra.
Soil application of organics has been explored as an alternative means of organic management of plant-parasitic nematodes. Efficiency of different oil-seed cakes of neem (Azadirachta indica), castor (Ricinus communis), groundnut (Arachis hypogaea), linseed (Linum usitatissimum), sunflower (Helianthus annuus) and soybean (Glycine max) were evaluated in field conditions with association of Pseudomonas fluorescens in relation to growth parameters of chickpea and population of plant-parasitic nematodes. Their efficacious nature was highly effective in reducing the population of these dominant soil nematodes. Significant improvement was observed in plant-growth parameters such as plant weight, percent pollen fertility, pod numbers, root-nodulation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chickpea; Oil-seed cakes; Organic management; Plant-parasitic nematodes; PGPR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600001

Gut: Key Element on Immune System Regulation
Provedor de dados: 52
Autores: Delgobo,Murilo; Paludo,Katia Sabrina; Fernandes,Daniel; Oliveira,Junior Garcia de; Ortolan,Gilberto Luiz; Favero,Giovani Marino.
Abstract The gut is the main organ that mediates the contact between antigens with our organism, controlling the immune response against environmental factors, such as microbiota and food. Innate lymphoid cells participate in the gut-associated lymphoid tissue (GALT) maturation during the prenatal and early postnatal periods. After birth, breast milk provides the essential elements for the continuity of development of this tissue, leading to structural changes and healthy microbiota installation. The microbiota participates in the organogenesis of the GALT, as in the formation of intestinal villi, stimulating the proliferation of stem cells and maintaining the integrity of epithelial barrier. Foods are also involved in maturation of the GALT, where the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunology; Gut; Peripheral Tolerance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100901

Morphoagronomic genetic diversity in american wild rice species
Provedor de dados: 52
Autores: Veasey,Elizabeth Ann; Silva,Edson Ferreira da; Schammass,Eliana Aparecida; Oliveira,Giancarlo Conde Xavier; Ando,Akihiko.
To characterize the genetic variability among species and populations of South American wild rice, eleven populations of Oryza glumaepatula, seven of O. grandiglumis, four of O. latifolia and one of O. alta, from Brazil and Argentina, were evaluated. A greenhouse experiment was conducted in completely randomized blocks with 23 treatments. Twenty morphoagronomic traits were assessed. Univariate analyses were performed with 16 quantitative traits with the partitioning of populations within species. Significant differences (p&lt;0.001) between species were observed for all the traits as well as among populations within the species. The most variable was O. glumaepatula followed by O. latifolia. Multivariate discriminant canonical and cluster analyses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Quantitative traits; Qualitative traits; Morphology; Oryza.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100012

Adsorption of natural dyes on clay fixed on polymers
Provedor de dados: 52
Autores: Lopes,Toni Jefferson; Gonçalves,Odinei Hess; Barros,Ricardo Barbosa; Capelli,Francine Carla Rodrigues; Machado,Ricardo Antonio Francisco; Quadri,Mara Gabriela Novy; Quadri,Marintho Bastos.
The main purpose of this work was to produce a pellet to minimize undesirable effects in fixed beds, such as colmatation, through clay fixation on the surface of polymeric particles. Preliminary experiments were carried out by heating the clay, namely Tonsil Terrana 580FF, in order to observe variations on its capacity to adsorb anthocyanins derived from red cabbage. Clays was fixed on five samples of high density polyethylene (HDPE) at 180°C -210°C for two hours. The morphological analyses of the resulting particles were accomplished through Scanning Electron Microscopy (SEM). The experiments demonstrated an increase on clay's adsorptive capacity of 15.65% at 120°C for 30 hours and 16.80% at 170°C for two hours. The SEM analysis showed that the clay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adsorption; Clay; Polymer; Anthocyanin/red cabbage.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400034

Bioethanol from Microalgal Biomass: A Promising Approach in Biorefinery
Provedor de dados: 52
Autores: Silva,Carlos Eduardo de Farias; Bertucco,Alberto.
Abstract The development of new technologies which increase the production of biofuel without directly compete with food production is required. Microalgal biomass has recently been in the highlight. The role of this biomass is here discussed within the concept of biorefinery and industrial sustainability of bioethanol production. The process of cultivation in order to accumulate around 50% of carbohydrates in the biomass (dry weight) and the importance of water and nutrient recycling are reviewed. Saccharification of biomass using enzymes or acids and alternative processes such as hydrothermal liquefaction and flash hydrolysis are addressed. Since the main monosaccharide in microalgal biomass is glucose, high rates of hydrolysis and fermentation were,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Microalgae; Biofuel; Hydrolysis; Nutrient recycle.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100602

Trypanosoma cruzi Chagas, 1909: genetic variability of isolates from chronic chagasic patients in the Paraná state, Brazil
Provedor de dados: 52
Autores: Kopp,Rogério Luiz; Miyazaki,Márcia; Thomaz-Soccol,Vanete.
The present work had as objective to verify the genetic diversity among strains of Trypanosoma cruzi isolated in chronic chagasic patients in the Paraná state. Fifty patients with compatible clinical symptoms were selected (cardiopathy, megacolon and/or megaesophagus) and that presented positive serological reaction to T. cruzi. Six strains of the protozoan were isolated in hemoculture and identified by electrophoresis in starch thick gel with aid of 14 isoenzymes (6PGD; G6PD; ME¹; ME²; ICD; PGM; GPI; GOT¹; GOT²; NP¹; NP²; DIA; MPI and FH). The statistical analysis were accomplished by the softwares NTSYs and UPGMA. Eighty-four electromorphs were individualized and four isoenzymes (ICD, GPI, 6PGD, e PGM) showed compatibility for heterozygosis. The phenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Multilocus enzyme electrophoresis; Chagas disease; Epidemiology; Phenetic analysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300009

Biogenic gases in tropical floodplain river
Provedor de dados: 52
Autores: Ballester,Maria Victória Ramos; Santos,José Eduardo dos.
Analysis of the distribution of biogenic gases in the floodplain of the Mogi-Guaçu River (São Paulo, Brazil) enabled the establishment of a "redox hierarchy", in which the main channel was the most oxidizing environment, followed by Diogo Lake, with Infernão Lake having the most reducing conditions of the subsystems evaluated. Diogo Lake exported about 853.4 g C.m-2.year-1, of which, 14.6% was generated from methanogenesis and 36.7% by aerobic respiration. For Infernão Lake, these values were 2016 g C.m-2.year-1, 1.8 % and 41.5 %, respectively. Carbon export by these systems was predominantly in the form of CO2, which was responsible for the release of 728.78 g C.m-2.year-1 at Diogo Lake and 1979.72 g C.m-2. year-1 at Infernão Lake. Such patterns may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biogenic gases; Methanogenesis; Aerobic respiration; CO2 production.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200006

Seasonal changes in the distribution and abundance of marine cladocerans of the Northwest Alboran Sea (Western Mediterranean), Spain
Provedor de dados: 52
Autores: Souza,Christiane Sampaio de; Mafalda Jr.,Paulo; Sallés,Soluna; Ramirez,Teodoro; Cortés,Dolores; Garcia,Alberto; Mercado,Jesus; Vargas-Yañez,Manuel.
The annual cycle of marine cladocerans was studied over six years from 1994 to 1999 within the frame of the monitoring Project ECOMALAGA at a nine stations in the NW of the Alboran Sea, with the aim of assessing seasonal patterns and interannual trends in distribution and abundance of marine cladocerans. Seven species (Penilia avirostris, Evadne nordmanni, Evadne spinifera, Pseudoevadne tergestina, Pleopis polyphemoides, Podon leuckarti and Podon intermedius) were detected in the northwest Alboran Sea. Total cladoceran relative abundance varied from 0 to 89 % of the total cladocerans. The abundance of cladocerans was higher in summer-autumn than in winter-spring (7012 - 4711.100 and 743 - 217.100m-3 , respectively). The species composition was very...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alboran Sea; Marine cladocerans; Distribution and abundance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300016

Dental pulp vascular permeability changes induced by dental bleaching
Provedor de dados: 52
Autores: Costa,Cristiane da; Miyagi,Sueli Patricia Harumi; Santos,Marcelo dos; Machado,Manoel Eduardo de Lima; Marques,Márcia Martins.
Aiming to compare the effect of different light sources for dental bleaching on vascular permeability of dental pulps, forty-eight incisors were used. The bleaching agent (35 % hydrogen peroxide) was activated by halogen light; LED (Light Emitting Diode) or LED, followed by laser phototherapy (LPT) (λ = 780 nm; 3 J/cm²). After the bleaching procedures, the animals received an intra-arterial dye injection and one hour later were sacrificed. The teeth were diaphanized and photographed. The amount of blue stain content of each dental pulp was quantified using a computer imaging program. The data was statistically compared (p &lt; 0.05). The results showed a significant higher (p &lt; 0.01) dye content in the groups bleached with halogen light, compared with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental pulp; Laser; LED; Tooth bleaching; Vascular permeability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100006

Feeding habits of capybaras (Hydrochoerus hydrochaeris, Linnaeus 1766), in the Ecological Reserve of Taim (ESEC - Taim) - south of Brazil
Provedor de dados: 52
Autores: Borges,Lucélia do Valle; Colares,Ioni Gonçalves.
The aim of this study was to determine feeding habitats of capybaras in the ESEC-Taim (RS, Brazil), using microhistological analyses of feces. Data were analyzed seasonally. In 172 samples analyzed, 17 species were identified, with predominance of Poaceae. Zizaniopsis bonariensis was the most frequent species in winter (38%), spring (32%) and summer (26.5%) and Paspalum disthichum in fall (31.5%). A observed increase in vegetation consumption during summer might be due to the food quality. Capybaras showed an opportunistic behavior in the winter and summer and a more selective behavior in spring and fall. Capybaras from the ESEC-Taim varied diet according to the season and alimentary availability.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capybara; Rodent; Herbivory; Diet; Poaceae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300007

The host marking pheromone application on the management of fruit flies - a review
Provedor de dados: 52
Autores: Silva,Márcio Alves; Bezerra-Silva,Gerane Celly Dias; Mastrangelo,Thiago.
The aim of this work was to review the role of the host marking pheromone (HMP) and its application in integrated management programs for the fruit flies. Initially the oviposition behavior of tephritids has been analyzed with emphasis on Ceratitis capitata. The deposition of HMP, which consists in the last stage of the oviposition behavior has been characterized and discussed about evolutive aspects and the biological meaning of the tephritidae communication through the HMP. Finally, the perspectives on the use of HMP in the integrated management of fruit flies have been discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oviposition behavior; Host marking pheromone; Oviposition deterring pheromone; IPM; Fruit flies.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600005

Determination of 99Mo contamination in a nuclear medicine patient submitted to a diagnostic procedure with 99mTc
Provedor de dados: 52
Autores: Dantas,Bernardo Maranhão; Dantas,Ana Letícia Almeida; Marques,Fábio Luiz Navarro; Bertelli,Luiz; Stabin,Michael G.
99mTc is a radionuclide widely used for imaging diagnosis in nuclear medicine. In Brazil it is obtained by elution from 99Mo-99mTc generators supplied by the Nuclear Energy Research Institute (IPEN). The elution is carried out in radiopharmacy laboratories located in hospitals and clinics. Depending of the quality of the generator and conditions of use during the elution process, 99Mo can be extracted from the column of the generator, becoming a radionuclidic impurity of the eluate used for the obtention of the radiopharmaceutical to be administered to the patient. 99Mo emits high-energy photons and beta particles and its presence degrades the quality of the image and unnecessarily increases the radiation dose delivered to the patient. An in-vivo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molybdenum 99; Technetium 99m; Nuclear medicine; Internal dosimetry; In vivo measurements; Whole body counter.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700032

Espaçamento para a cultura da Mandioca
Provedor de dados: 52
Autores: Takahashi,Mário; Guerini,Valdir Luis.
The present work had the objective of evaluating the effect of different line spacing upon some agronomic parameters of cassava (Manihot esculenta Crantz) culture. The experiment was conducted in county of Palotina, in the west region of Paraná State, Brazil. The studied spacing were 0.8, 1.0, 1.4 e 1.6 m between lines and 0.6 m between plants, which rendered population densities of 20,830, 16,660, 13,880, 11,900 and 10,410 plants/ha. For the production of fresh and dry root matter and stem fresh matter, the best space between lines was 1.0 m. The highest number of tuberous roots per plant, obtained with the space of 1.40 m between lines did not guarantee the highest productivity of root matter/ha. The production of the vegetative portion was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Manihot esculenta; Spacing; Plant populations.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400014

Morphology and anatomy of the flower and anthesis of Metrodorea nigra St. Hill. (Rutaceae)
Provedor de dados: 52
Autores: Souza,Luiz A. de; Moscheta,Ismar S.; Mourão,Káthia S. M.; Rosa,Sonia M. da.
Studies on the morphology and anatomy of the flower and anthesis of Metrodorea nigra St. Hill. (Rutaceae) showed perfect flowers, which occurred in panicle. The peak of the flowering was August and September. The anthesis was diurnal and the species presented protandrous flowers. The green sepals and the red petals presented papillose epidermis with stomata and parenchymatic mesophyll. The tetrasporangiate anther presented a papillose epidermis too, endothecium cells with secondary parietal thickenings, two middle layers and binucleate tapetum. The only pistil presented an ovary wall with nectariferous tissue and a meristematic ventral epidermis, a solid style and an inconspicuous stigma. The ovules were anatropous, bitegmic and crassinucelate and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metrodorea nigra; Rutaceae; Blossom; Anthesis; Protandry; Flower structure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100014

Anticholinesterase effect of eserine (physostigmine) in fish and crustacean species
Provedor de dados: 52
Autores: Monserrat,José M.; Bianchini,Adalto.
The kinetic characteristic (Km) of cholinesterase from the crab Chasmagnathus granulata, the shrimp Farfantepenaeus paulensis and the fish Odontesthes bonaeriensis were compared and correlated with the anticholinesterasic effect of eserine (physostigmine). For the crustaceans, the estimated Km values were about 5-8 times higher than that estimated for the fish (0.04 mM). In the crab and the shrimp, the concentration of eserine which inhibited 50% of cholinesterase activity (IC50) was estimated as 5.33x10-4 and 4.33x10-4 mM, respectively. In both cases, it was significantly higher (P &lt; 0.05) than that estimated for the fish larvae (7.43x10-5 mM). A high Km could reflect a lower affinity of the cholinesterase for its natural substrate, acetylcholine, or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eserine; Physostigmine; Acetylcholinesterase; Fish; Silverside fish; Crustacean.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100009

Acetylsalicylic acid and labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Fonseca,Adenilson de Souza da; Frydman,Jacques Natan Grinapel; Rocha,Vanessa Câmara da; Bernardo-Filho,Mario.
Acetylsalicylic acid is the drug most used an anti-inflammatory agent and for secondary prevention of thrombotic phenomenon. Drugs can modify the labeling of blood constituents with technetium-99m (99mTc). The aim of this work was to evaluate the effect of in vitro or in vivo assays with acetylsalicylic acid on the labeling of the blood constituents with 99mTc. In vitro assay was performed with samples of whole blood from Wistar rats incubated with acetylsalicylic acid (1.0 mg/ml) for one hour before the 99mTc-labeling process. For in vivo assay, Wistar rats were treated with acetylsalicylic acid (1.5 mg/kg) during one hour, and the whole blood was withdrawn for the 99mTc-labeling process. Saline was used in control groups. Data showed that the fixation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylsalicylic Acid; Blood Constituents; Technetium-99m.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700024

Production and Characterization of IgY against Canine IgG: Prospect of a New Tool for the Immunodiagnostic of Canine Diseases
Provedor de dados: 52
Autores: Santos,Fernanda Nunes; Brum,Beatriz Coutinho; Cruz,Paula Borba; Molinaro,Claudia Moraes; Silva,Valmir Laurentino; Chaves,Sérgio Augusto de Miranda.
This study describes the production of a new avian polyclonal antibody (IgY) against canine IgG, as another tool for the immunodiagnostic using IgY technology. The immunization protocol caused neither deaths nor pathologies, and no decline in egg laying capacity was detected. The total concentration of isolated IgY was constant, without significant difference (p&gt; 0.05), with average of 97.55 mg of IgY/yolk. The IgY revealed a strong sensitivity and specificity in recognition against canine IgG by ELISA. After the immunization, there was a significant increase in the production of IgY specific from the first to the second month (p &lt;0.05), reaching a stable peak without decrease in the production by the end of the analysis period (p &gt; 0.05). The IgY...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY polyclonal antibodies; Chicken antibodies; Hen immunization; Canine IgG.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400523

Filamentous fungi isolated from Brazilian semiarid tolerant to metallurgical industry wastes: an ex situ evaluation
Provedor de dados: 52
Autores: Silva Júnior,Flavio Manoel Rodrigues da; Pereira,Sônia Valéria.
The purpose of this study was to assess the impact of metallurgical industry wastes on the semiarid soil microbiota using physico-chemical and microbiological parameters, highlighting the filamentous fungi assembly. Soil samples were collected in an area of industrial waste deposit contaminated with lead and mixed with natural soil (control soil) in seven different concentrations (0, 7.5, 15, 30, 45, 60 and 100%). The results showed alterations on the physico-chemical properties of the soil treated with industrial wastes, with a gradate increase of the soil's pH (5.6-10.4) and electrical conductivity (0.3-14.7 dS m-1) and also reduction of organic matter (7.0-1.8%). The use of microbiological parameters (fungal richness and diversity, CO2emission, and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chaetomium; Microbial activity; Contaminated soils; Filamentous fungi; Lead.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500723

Effects of Yucca Extract on Feed Efficiency, Immune and Antioxidative Functions in Broilers
Provedor de dados: 52
Autores: Su,Jun-Ling; Shi,Bin-Lin; Zhang,Peng-Fei; Sun,Deng-Sheng; Li,Ti-Yu; Yan,Su-Mei.
Effects of yucca extract (YE) on feed efficiency, immune and antioxidative function in Arbor Acres broilers were studied. One hundred and twenty-eight fourteen-day-old broiler chickens were randomly divided into four treatments with four replicates of 8 birds each. These four diets were formulated by adding 0, 100, 200 and 300 mg/kg YE to the basal diet. The results showed that: diets supplemented with 100 and 200 mg/kg YE increased average body weight gain, feed efficiency, IgG, IgM, T-AOC, CAT and SOD levels, and have positive effects on inducing immune organs' maturation. In addition, 100 mg treatment mainly improved the feed efficiency whereas 200 mg treatment mainly acted on immunity and anti-oxidation. In conclusion, YE can be used as a feed additive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yucca product; Chicken; Feed efficiency; Immunity; Antioxidant.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100504

Enzymatic hydrolysis of sugarcane bagasse pretreated with acid or alkali
Provedor de dados: 52
Autores: Pietrobon,Vivian Cristina; Monteiro,Regina Teresa Rosim; Pompeu,Georgia Bertoni; Borges,Eduardo Poggi e; Lopes,Mario Lucio; Amorim,Henrique Vianna de; Cruz,Sandra Helena da; Viégas,Ellen Karine Diniz.
The aim of this study was to evaluate the performance of enzymatic hydrolysis of acid or alkali pretreated sugarcane bagasse for the production of fermentable sugars. The first step consisted of selection of commercial enzymes presenting the highest cellulolytic activities. After selection of four enzymes: HPL, CL, P1 and P4, their performances were tested in the bagasse pretreated with acid and alkali. The sugar content of the hydrolysates was analyzed by anion exchange liquid chromatography. Data showed that the joint action of 0.5% acid pretreatment, 121ºC, 30 minutes and enzyme CL provides the best results, 67.25 g of hexose and 148.13g of pentose per kg of dry bagasse.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bagasse sugarcane; Hydrolysis; Cellulolytic enzymes; HPAEC.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200002

Microbiology, sensory evaluation and shelf life of irradiated chicken breast fillets stored in air or vacuum
Provedor de dados: 52
Autores: Mantilla,Samira Pirola Santos; Santos,Érica Barbosa; Vital,Helio de Carvalho; Mano,Sérgio Borges; Freitas,Mônica Queiroz de; Franco,Robson Maia.
This work investigated the effects of different packaging methods (air and vacuum) combined with irradiation (0.0, 2.0 and 3.0 kGy) on the preservation of chicken breast fillets stored at 1ºC for up to 18 days by sensorial test, determination of pH and bacterial growth. The findings indicated that the post-irradiation lag phase increased with the dose, leading to an extension in shelf-life. Vacuum-packed samples irradiated at 3.0 kGy exhibited the longest shelf life. Among the analyzed bacteria, coliforms and Listeria spp. were most sensitive to gamma radiation. All the fillets acquired more attractive coloration and better overall impression with irradiation. The combined use of vacuum packaging and irradiation (3.0 kGy) reduced the microbial populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irradiation; Chicken fillets; Modified atmosphere; Microbiology; Sensory tests.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300019

Radiotherapy, cancer and some anatomical structures related with the pelvic floor: an evaluation of the scientific literature
Provedor de dados: 52
Autores: Santos-Filho,Sebastião David; Presta,Giuseppe Antonio; Paoli,Severo de; Missailids,Sotiris; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Radiotherapy is a cancer treatment that uses high-energy X-rays or other types of ionizing radiation to try to kill the cancer cells in various organs/tissues. PubMed is a database used as a tool in various publications. The aim of this work was to evaluate, in the PubMed, the number of publications (NP) in radiotherapy and cancer in various organs related to the pelvic floor as well as brachytherapy. The searches were performed (August 2008) in the PubMed (http://www.pubmed.com) using the words: (i) radiotherapy (RT) or cancer (CA), (ii) RT and CA, (iii) RT and CA and prostate, RT and CA and testis, RT and CA and breast, RT and CA and kidneys, RT and CA and vulva, RT and CA and vagina, RT and CA and penis, RT and CA and uterus, RT and CA and ovary, RT and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiotherapy; Cancer; Pelvic floor; PubMed; Scientific interest.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700008

Floral Diversity in Different Types of Honey
Provedor de dados: 52
Autores: Haidamus,Susana Linhares; Lorenzon,Maria Cristina Affonso; Koshiyama,Adriano Soares; Tassinari,Wagner de Sousa.
Abstract Many different types of honey are available in the Brazilian market. They vary in color, flavor, smell, thereby increasing interest in honey characterization relating to botanical origin. A total of 155 honey samples belonging to Brazilian flora were examined on the pollen analysis; sampling is made in a span of one year. The preparation followed melisso palynological analysis based on the specific botanical variety. The pollen spectra revealed 60 pollen types belonging to 27 plant families and Myrtaceae, Fabaceae and Asteraceae were the dominant plant families. Few pollen types were found in most samples of honey. The families that showed major richness of pollen types were Fabaceae and Asteraceae. Only six floral sources of pollen plants and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant diversity pollen analysis trophic niche Ecology; Conservation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100221

Influence of Brettanomyces custersianus upon the activity of Saccharomyces cerevisiae strains during the tumultuous phase of vinification
Provedor de dados: 52
Autores: Silva,Gildo Almeida da; Poletto,Carolina Madalozzo; Poli,Jandora Severo; Valente,Patricia.
The aim of this work was to evaluate the influence of Brettanomyces custersianus on the metabolic activity of Saccharomyces cerevisiae during the tumultuous stage of wine production. The Cabernet Sauvignon grape must with the skin was inoculated with individual cultures of Sacch. cerevisiae and with mixed cultures of Sacch. cerevisiae and Br. custersianus. During the 6-day tumultuous phase of fermentation, the highest ethanol production and the highest sugar consumption were obtained with the strains without B. custersianus. Fermentations carried out with the addition of Brettanomyces metabolites, acetic acid and 4-ethylphenol, showed that only the former inhibited the growth of both Sacch. cerevisiae strains used. In some cases, Br. custersianus could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dekkera/Brettanomyces; Wine; Vinification; Custer effect.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200017

Diet of Astyanax species (Teleostei, Characidae) in an Atlantic Forest River in Southern Brazil
Provedor de dados: 52
Autores: Vilella,Fábio Silveira; Becker,Fernando Gertum; Hartz,Sandra Maria.
Feeding habits of six species of Astyanax from river Maquiné are described. Fishes were sampled bi-monthly from November/95 to September/96 in two zones of the river. Items were identified, counted and had their abundance estimated according to a semi-quantitative scale. Frequency of occurrence, alimentary importance index (IFI) values and a similarity analysis of diets for each species-river zone sample were examined. All the species were considered typically omnivorous, with insects and vegetal matter being the most important items in their diet. These species could act as seed dispersers, particularly for macrophytes. Intra-specific spatial differences were not observed in comparisons of samples from two diferent regions of the river, except for A....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diet; Seed dispersal; Fish; Astyanax; Atlantic Forest; Brazil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200015

40K, 226Ra AND 228Ra SERIES IN BOVINE AND POULTRY FEED AND IN DICALCIUM PHOSPHATE (DCP) SAMPLES BY GAMMA-RAY SPECTROMETRY
Provedor de dados: 52
Autores: Luz Filho,Isaias Venancio da; Scheibel,Viviane; Appoloni,Carlos Roberto.
The aim of this study is to determine the amounts of radionuclides' activity present in samples of feed for young and adult chicken and feed for dairy and beef cattle, as well as in dicalcium phosphate (DCP) (CaHPO4), used as a nutritional supplement for animal feed. Radioactivity in these samples is due to the presence of the 238U and 232Th radioactive series, as well as their daughter. In addition, as it is for environmental samples, the activity of 40K should be an important source of radioactivity in all analyzed samples. Gamma rays were measured using a standard spectroscopy system, with a high-resolution HPGe detector. Measured activities in feed samples ranged from 0.23 to 1.51 Bq.kg-1, 0.67 to 4.21 Bq.kg-1, 0.29 to 1.63 Bq.kg-1 and 236 to 402...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma Ray Spectrometry; Natural radioactivity; Animal Feed; Dicalcium phosphate (DCP); Phosphate fertilizers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100506