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| Registros recuperados: 56 | |
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| Cochennec-laureau, Nathalie; Baud, Jean-pierre; Pepin, Jean-francois; Benabdelmouna, Abdellah; Soletchnik, Patrick; Lupo, Coralie; Garcia, Celine; Arzul, Isabelle; Boudry, Pierre; Huvet, Arnaud; Pernet, Fabrice; Bachere, Evelyne; Bedier, Edouard; Petton, Bruno; Gaussem, Florian; Stanisiere, Jean-yves; Degremont, Lionel. |
| Depuis 2008, la filière ostréicole française doit faire face à des surmortalités exceptionnelles des naissains d’huîtres creuses, Crassostrea gigas, (huîtres de moins d’un an) comprises entre 60 et 90% dans tous les sites d’élevage. Des cas de mortalité similaires ont été décrits en Irlande, dans les îles anglo-normandes, au Portugal en 2009 et au Royaume-Uni en 2010. Récemment deux épisodes de mortalité, associés à la présence d’un virus OsHV1 μvar (ou un variant très proche) ont été également décrits en Nouvelle Zélande (décembre 2010) et en Australie (janvier 2011) soulignant la vulnérabilité de la filière ostréicole dans le monde. L’objectif de ce document est de faire un point sur les recherches menées depuis l’apparition de ce phénomène et de... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14423/11719.pdf |
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| Gonzalez, Marcelo; Gueguen, Yannick; Destoumieux Garzon, Delphine; Romestand, Bernard; Fievet, Julie; Pugniere, M; Roquet, F; Escoubas, Jean-michel; Vandenbulcke, F; Levy, O; Saune, Laure; Bulet, P; Bachere, Evelyne. |
| A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LIPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle,... |
| Tipo: Text |
Palavras-chave: Oyster innate immunity; Mollusk; Hemocyte; Epithelia; Antimicrobial. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3564.pdf |
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| Destoumieux, Delphine; Bulet, Philippe; Loew, Damarys; Van Dorsselaer, Alain; Rodriguez, Jenny; Bachere, Evelyne. |
| We report here the isolation of three members of a new family of antimicrobial peptides from the hemolymph of shrimps Penaeus vannamei in which immune response has not been experimentally induced. The three molecules display antimicrobial activity against fungi and bacteria with a predominant activity against Gram-positive bacteria. The complete sequences of these peptides were determined by a combination of enzymatic cleavages, Edman degradation, mass spectrometry, and cDNA cloning using a hemocyte cDNA library. The mature molecules (50 and 62 residues) are characterized by an NH2-terminal domain rich in proline residues and a COOH-terminal domain containing three intramolecular disulfide bridges. One of these molecules is post-translationally modified by... |
| Tipo: Text |
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| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00335/44649/57344.pdf |
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| Bachere, Evelyne; Chagot, Dominique; Tige, Gilbert; Grizel, Henri. |
| Considering the epizootics which affect the French stock of flat oysters, Ostrea edulis (Grizel, 1985), trials to introduce an Australian oyster, Ostrea angasi, have been undertaken. The spat produced in a hatchery from breeders imported from Australia were reared in the south ofBrittany and in Normandy (Bougrier et al., 1986). Following mass mortalities (65% of individuals) a haplosporidian was observed in this species. Sporogenesis of the parasite was studied using Iight and electron microscopy. |
| Tipo: Text |
Palavras-chave: Haplosporidian; Parasitology; Pathology; Ostrea angasi; Oysters. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/1986/acte-3112.pdf |
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| Rondon Sallan, Rodolfo; Grunau, Christoph; Fallet, Manon; Charlemagne, Nicolas; Sussarellu, Rossana; Chaparro, Cristian; Montagnani, Caroline; Mitta, Guillaume; Bachere, Evelyne; Akcha, Farida; Cosseau, Celine. |
| Environmental epigenetic is an emerging field that studies the cause-effect relationship between environmental factors and heritable trait via an alteration in epigenetic marks. This field has received much attentions since the impact of environmental factors on different epigenetic marks have been shown to be associated with a broad range of phenotypic disorders in natural ecosystems. Chemical pollutants have been shown to affect immediate epigenetic information carriers of several aquatic species but the heritability of the chromatin marks and the consequences for long term adaptation remain open questions. In this work, we investigated the impact of the diuron herbicide on the DNA methylation pattern of spat from exposed Crassotrea gigas genitors. This... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Mollusk; DNA methylation; Herbicide; Epigenetic. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00602/71413/69895.pdf |
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| Akcha, Farida; Barranger, Audrey; Bachere, Evelyne; Berthelin, C. Heude; Piquemal, D.; Alonso, P.; Sallan, R. Rondon; Dimastrogiovanni, G.; Porte, C.; Menard, Dominique; Szczybelski, A.; Benabdelmouna, Abdellah; Auffret, M.; Rouxel, Julien; Burgeot, Thierry. |
| Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation... |
| Tipo: Text |
Palavras-chave: Oyster; Pesticide; Reproduction; Histology; Gene expression; RT-qPCR; Steroid; Immunity. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00309/41983/83232.pdf |
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| Saavedra, Carlos; Bachere, Evelyne. |
| Interest in bivalve genomics has emerged during the last decade, owing to the importance of these organisms in aquaculture and fisheries and to their role in marine environmental science. Knowledge of bivalve genome structure, function and evolution resulting from 20th century "single gene" approaches is limited, but genomic technologies are called to dramatically increase it. Research based on linkage maps, transcriptomics and proteomics is being carried out to study the genetic and molecular bases of traits of interest in bivalve farming industry, mainly disease susceptibility, tolerance to environmental stress, and growth. The Pacific oyster (Crassostrea gigas) is now the focus of an international genome-sequencing consortium. The use of bivalves in... |
| Tipo: Text |
Palavras-chave: Shellfish toxins; Feeding; Stress; Proteomics; Genomics; Bivalves. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1709.pdf |
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| Bachere, Evelyne; Grizel, Henri. |
| A new Haplosporidie has been observed in the flat oyster Ostrea edulis from the Mediterranean Sea. Different development stages have been described in light and electron microscopy. The characteristics of the spores and especially the presence of at least four sporal filaments enable us to distinguish this Haplosporidie from those already described in the Atlantic flat oysters. Different haplosporidies have been described in the oyster. Some are known as being pathogenic, Minchinia costalis (Andrews et al., 1962 and 1978; Couch 1967) and Minchinia nelsoni (Haskin et al., 1966; Farley 1968 and 1975), others, identified only recently, are considered as occasional parasites (Van Banning, 1977). During the research conducted on Bonamia ostreae (Pichot et al.,... |
| Tipo: Text |
Palavras-chave: Pathologie; Haplosporidium; Parasite; Ostrea edulis; Huître plate. |
| Ano: 1982 |
URL: http://archimer.ifremer.fr/doc/1982/publication-6576.PDF |
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| Pilotto, Mariana R.; Goncalves, Andre N. A.; Vieira, Felipe N.; Seifert, Walter Q.; Bachere, Evelyne; Rosa, Rafael D.; Perazzolo, Luciane M.. |
| We provide a global overview of the intestinal bacteriome of Litopenaeus vannamei in two rearing systems and after an oral challenge by the White spot syndrome virus (WSSV). By using a high-throughput 16S rRNA gene sequencing technology, we identified and compared the composition and abundance of bacterial communities from the midgut of shrimp reared in the super-intensive biofloc technology (BFT) and clear seawater system (CWS). The predominant bacterial group belonged to the phylum Proteobacteria, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes. Within Proteobacteria, the family Vibrionaceae, which includes opportunistic shrimp pathogens, was more abundant in CWS than in BFT-reared shrimp. Whereas the families Rhodobacteraceae and... |
| Tipo: Text |
Palavras-chave: Biofloc technology (BFT); Penaeid shrimp; Gut microbiota; White spot syndrome virus; 16S rRNA gene sequencing. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00454/56546/58256.pdf |
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| Yang, Yinshan; Boze, Helene; Chemardin, Patrick; Padilla, Andre; Moulin, Guy; Tassanakajon, Anchalee; Pugniere, Martine; Roquet, Francoise; Destoumieux Garzon, Delphine; Gueguen, Yannick; Bachere, Evelyne; Aumelas, Andre. |
| The anti-lipopolysaccharide factor ALF-Pm3 is a 98-residue protein identified in hemocytes from the black tiger shrimp Penaeus monodon. It was expressed in Pichia pastoris from the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter as a folded and N-15 uniformly labeled rALF-Pm3 protein. Its 3D structure was established by NMR and consists of three alpha-helices packed against a four-stranded beta-sheet. The C-34-C-55 disulfide bond was shown to be essential for the structure stability. By using surface plasmon resonance, we demonstrated that rALF-Pm3 binds to LPS, lipid A and to OM(R)-174, a soluble analogue of lipid A. Biophysical studies of rALF-Pm3/LPS and rALF-Pm3/OM(R)-174 complexes indicated rather high molecular sized aggregates, which... |
| Tipo: Text |
Palavras-chave: Ultracentrifugation; Surface plasmon resonance; Septic shock; Structure; NMR; Lipid A; Lipopolysaccharide; Anti lipopolysaccharide factor. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6320.pdf |
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| Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis. |
| The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These... |
| Tipo: Text |
Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf |
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| Rosa, Rafael D.; Santini, Adrien; Fievet, Julie; Bulet, Philippe; Destoumieux-garzon, Delphine; Bachere, Evelyne. |
| Background: Big defensin is an antimicrobial peptide composed of a highly hydrophobic N-terminal region and a cationic C-terminal region containing six cysteine residues involved in three internal disulfide bridges. While big defensin sequences have been reported in various mollusk species, few studies have been devoted to their sequence diversity, gene organization and their expression in response to microbial infections. Findings: Using the high-throughput Digital Gene Expression approach, we have identified in Crassostrea gigas oysters several sequences coding for big defensins induced in response to a Vibrio infection. We showed that the oyster big defensin family is composed of three members (named Cg-BigDef1, Cg-BigDef2 and Cg-BigDef3) that are... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00050/16096/13576.pdf |
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| Simon-colin, Christelle; Gueguen, Yannick; Bachere, Evelyne; Kouzayha, Achraf; Saulnier, Denis; Gayet, Nicolas; Guezennec, Jean. |
| Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Exopolysaccharide; Tachyplesin; Antimicrobial. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00275/38599/37128.pdf |
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| Registros recuperados: 56 | |
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