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Enhanced tethered-flight duration and locomotor activity by overexpression of the human gene SOD1 in Drosophila motorneurons Genet. Mol. Biol.
Petrosyan,Agavni; Hsieh,I-Hui; Phillips,John P.; Saberi,Kourosh.
Mutation of the human gene superoxide dismutase (hSOD1) is associated with the fatal neurodegenerative disease familial amyotrophic lateral sclerosis (Lou Gehrig’s disease). Selective overexpression of hSOD1 in Drosophila motorneurons increases lifespan to 140% of normal. The current study was designed to determine resistance to lifespan decline and failure of sensorimotor functions by overexpressing hSOD1 in Drosophila‘s motorneurons. First, we measured the ability to maintain continuous flight and wingbeat frequency (WBF) as a function of age (5 to 50 days). Flies overexpressing hSOD1 under the D42-GAL4 activator were able to sustain flight significantly longer than controls, with the largest effect observed in the middle stages of life. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Behavioral genetics; Superoxide dismutase; Drosophila; Motorneuron; Longevity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100107
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Activity of antioxidative enzymes in watercress and Chinese cabbage plants grown under hydroponic system with brackish water Horticultura Brasileira
Lira,Raquele M; Silva,Ênio FF; Willadino,Lilia; Oliveira Filho,Ronaldo A; Andrade,Gilvany R.
ABSTRACT Underground water reserves in Semiarid Region present high salinity levels; however, scarcity of resource compels farmers to use this water for conventional agriculture. Biomass production and activity of the antioxidative defense system of Chinese cabbage and watercress were evaluated in plants grown under hydroponic NFT system with brackish water. The experimental design was completely randomized, with four replications and five water salinity levels (1.95; 3.37; 4.48; 6.82 and 7.51 dS m-1), obtained by an increasing addition of NaCl into the local water supply and nutrient salts for preparation of nutrient solution. Both watercress and Chinese cabbage plants decreased in fresh and dry shoot biomass, in relation to increase of salinity in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Naturtium officinale; Brassica pekinensis; Superoxide dismutase; Ascorbate peroxidase; Catalase.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000200205
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Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat ArchiMer
Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco.
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides...
Tipo: Text Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf
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Etude de la réponse immunitaire chez l'huître Crassostrea gigas : caractérisation et fonctions de protéines de reconnaissance aux LPS et d'effecteurs antimicrobiens ArchiMer
Gonzalez, Marcelo.
Research on the immune response of oysters is motivated by the problems of infectious diseases potentially found in the farms. Over the course of the last few years, the development of genomic approaches has allowed us to make headway on characterising genes involved in the cup oyster's (Crassostrea gigas) innate immunity, particularly thanks to various programmes for analysing transcriptome, of type EST (Express Sequence Tag) and subtractive banks. Within the context of this work, over the course of which we have used the data from genomic research and we have also developed biochemical approaches, we were particularly interested in studying the proteins involved in reconnaissance reactions to LPS as well as to antimicrobial effectors. In an earlier part,...
Tipo: Text Palavras-chave: Defense peptides; Polysaccharides; LPS; Hemocytes; Immune response; Crassostrea gigas; Shellfish culture; LPS; Défensine; Peptides antimicrobiens; LBP; BPI; Superoxide dismutase; Hémocytes; Crassostrea gigas; Huître; Mollusque; Immunité.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2098.pdf
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Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas ArchiMer
Harney, Ewan; Artigaud, Sebastien; Le Souchu, Pierrick; Miner, Philippe; Corporeau, Charlotte; Essid, Hafida; Pichereau, Vianney; Nunes, Flavia.
Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40 h under either control (pH 8.1, 20 °C), acidified (pH 7.9, 20 °C), warm (pH 8.1, 22 °C) or warm acidified (pH 7.9, 20 °C) conditions. Larvae in acidified conditions were significantly smaller than in the control, but warm acidified conditions mitigated negative effects on size, and increased calcification. A proteomic approach employing two-dimensional electrophoresis (2-DE) was used to quantify proteins and relate their abundance to phenotypic traits. In...
Tipo: Text Palavras-chave: Crassostrea gigas; Ocean acidification; Larval development; ATP synthase; GAPDH; Superoxide dismutase.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00299/40985/40076.pdf
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Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection ArchiMer
Le Bris, Cedric; Richard, Gaelle; Paillard, Christine; Lambert, Christophe; Seguineau, Catherine; Gauthier, Olivier; Pernet, Fabrice; Guerard, Fabienne.
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection....
Tipo: Text Palavras-chave: Venerupis philippinarum; Vibrio tapetis; Immunity; Phenoloxidase; Superoxide dismutase.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00221/33257/31733.pdf
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Seasonal changes in mRNA encoding for cell stress markers in the oyster Crassostrea gigas exposed to radioactive discharges in their natural environment ArchiMer
Farcy, Emilie; Voiseux, Claire; Lebel, Jean-marc; Fievet, Bruno.
The North Cotentin area (Normandy, France) hosts several nuclear facilities among which the AREVA reprocessing plant of La Hague is responsible for controlled discharges of liquid radioactive wastes into the marine environment. The resulting increase in radioactivity is very small compared to natural radioactivity. However, concerns about environment protection prompted the scientific community to focus on the effects of the chronic exposure to low concentrations of radionuclides in non-human biota. This study contributes to the evaluation of the possible impact of radioactive discharges on the oyster Crassostrea gigas in the field. Real-time polymerase chain reaction was used to quantify the expression levels of genes involved in cell stress in the...
Tipo: Text Palavras-chave: Crassostrea gigas; Superoxide dismutase; Metallothionein; Heat shock protein; MRNA; Seasonal variations; Radioactive discharges.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2601.pdf
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Contents of Pigments and Activity of Antioxidant Enzymes in Rice Plants Pre-Treated with Sodium Nitroprusside and Exposed to Clomazone Planta Daninha
SILVA,K.S.; TABALDI,L.A.; ROSSATO,L.V.; CAVICHIOLI,B.M.; BASÍLIO,V.B.; MACHADO,S.L.O..
ABSTRACT: The pre-treatment of rice seeds with sodium nitroprusside (SNP) was used to investigate the effect of exogenous nitric oxide on the pigment content and the activity of antioxidant enzymes during the inhibition of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway by the herbicide clomazone. The seeds were soaked in SNP solution (200 mM) for one and 10 hours and sprayed with clomazone, at post-seeding and at the needle-point, with 612 (experiment 1) and 1,224 g a.i. ha-1 (experiment 2). The control of both factors received distilled water. Carotenoids, chlorophylls a, b and total, superoxide dismutase (SOD), guaiacol peroxidase (POD) and the correlation between the variables were evaluated in the shoot of the plants. When compared to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorophyll; Carotenoids; Oryza sativa; Superoxide dismutase; Guaiacol peroxidase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100231
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Activity of Antioxidant Enzymes in Euphorbia heterophylla Biotypes and their Relation to Cross Resistance to ALS and Protox Inhibitors Planta Daninha
XAVIER,E.; TREZZI,M.M.; OLIVEIRA,M.C.; VIDAL,R.A.; BRUSAMARELLO,A.P..
ABSTRACT: The characteristics of multiple resistance in Euphorbia heterophylla biotypes to herbicides that are inhibitors of ALS (Acetolactate synthase) and PPO (Protoporphyrinogen oxidase) and their responsible mechanisms are still not completely elucidated. The objectives of this study were to identify cross-resistance to herbicides from different chemical groups of ALS inhibitors (imidazolinones, sulfonylureas, pyrimidyl benzoates and sulfonanilides) and also PPO inhibitors (diphenylethers, phthalamides, oxadiazoles, triazolinones and pyrimidinediones) in E. heterophylla biotypes with multiple resistance to these herbicides; to analyze whether the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD) constitute mechanisms that are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dose-response curves; Superoxide dismutase; Peroxidase; Acetolactate synthase; Protoporphyrinogen oxidase.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100340
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Effect of potassium sources on the antioxidant activity of eggplant¹ Rev. Bras. Ciênc. Solo
Marques,Douglas José; Broetto,Fernando; Ferreira,Mozart Martins; Lobato,Allan Klynger da Silva; Ávila,Fabricio William de; Pereira,Fabricio José.
Potassium participates in the essential processes in plant physiology, however, the effects of K sources on plant metabolism have been little studied. Also, in certain cases, K sources and concentrations may cause undesirable effects, e.g., soil salinization. The objective was to evaluate the effect of K sources and levels on the enzyme activity of the antioxidant system and protein content in eggplant (Solanum melongena L.) leaves and to determine the most suitable K sources for these physiological characteristics. The experiment was conducted in randomized blocks, in a 2 × 4 factorial design, consisting of two K sources (KCl and K2SO4) and rates (250, 500, 750, and 1000 kg ha-1 K2O), with four replications. The following variables were evaluated: plant...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Solanum melongena L.; Potassium; Superoxide dismutase; Catalase; Protein.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832014000600018
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Effects of dietary propolis and vitamin E on growth performance and antioxidant status in blood of juvenile Rainbow trout, Oncorhynchus mykiss (Teleostei: Salmoniformes) under different flow rates Rev. Bras. Zool.
Kelestemur,Gülüzar Tuna; Seven,Pinar Tatli; Yilmaz,Seval.
The present study investigated the effects of propolis and vitamin E supplementation in diets of juvenil rainbow trout subjected to two different flow rates with or without flow stress (0.9 and 2.1 l/min, respectively) on final weigth (FW), condition factor (CF), feed conservation ratio (FCR), protein efficiency ratio (PER) and vitamin A, C and E concentrations and malondialdehyde (MDA) levels in serum as well as plasma superoxide dismutase (SOD) activity. The experimental groups were as follows: group C was fed a basal diet, group P10 was fed a basal diet supplemented with 10 g propolis/kg, group P30 was fed a basal diet supplemented with 30 g propolis/kg, group E60 was fed a basal diet supplemented with 60 mg vitamin E/kg. CF and PER were not different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipid peroxidation; Rainbow trout; Superoxide dismutase; Vitamin E.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702012000200001
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Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture Scientia Agricola
Pompeu,Georgia Bertoni; Gratão,Priscila Lupino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.
Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Catalase; Nickel; Superoxide dismutase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015
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Enzymatic antioxidant responses to biostimulants in maize and soybean subjected to drought Scientia Agricola
Vasconcelos,Ana Carolina Feitosa de; Zhang,Xunzhong; Ervin,Erik H.; Kiehl,Jorge de Castro.
Water stress is one of the most important environmental factors inducing physiological changes in plants, such as decrease in the water potential of the cells, the stomatal closure; and the development of oxidative processes mediated by reactive oxygen species (ROS). Antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) are efficient scavengers of ROS. The aim of this research was to examine how the application of biostimulant based on humic substances and aminoacids may affect activity levels of SOD, CAT, and APX of maize and soybean plants under well-watered or drought stress conditions. Pots (4.5 L) were filled with a Typic Hapludult soil where the biostimulants doses were applied. It was taken leaf samples in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superoxide dismutase; Catalase; Ascorbate peroxidase; Water status.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300015
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กิจกรรมเอนไซม์ซูเปอร์ออกไซด์ดิสมิวเทสในหัวแก่นตะวันสายพันธุ์ต่างๆ Thai Agricultural
Anoma Dongsansuk; Sanan Jogloy.
The study of SOD activity in Jerusalem artichoke tubers in 3 varieties that were different harvesting stage and no report the amount of SOD in different parts of Jerusalem artichoke tuber was carried out. The aims of this study were to evaluate SOD activity in various varieties of Jerusalem artichoke and to evaluate the difference of SOD activity in different parts of Jerusalem artichoke tuber. This experiment used 3 varieties of Jerusalem artichoke including Hel 65, CN 52867 and JA 89 that were different genotypes. These tubers were separated into 3 parts: head, middle and tail for extraction and determination of SOD activity. The results showed that Jerusalem artichoke var. Hel 65 exhibited the highest SOD activity compared to var. CN 52867 and JA 89....
Tipo: PhysicalObject Palavras-chave: Helainthus tuberosus; Jurusalem artichoke; Enzyme; Antioxidant enzyme; Superoxide dismutase; SOD; Genotypes; แก่นตะวัน; เยรูซาเลม อาร์ติโชค; การเปรียบเทียบพันธุ์; เอนไซม์; ซูเปอร์ออกไซด์ดิสมิวเทส; แอนติออกซิแดนท์.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5735
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Antioxidant capacity in relationship to serum lipid peroxides levels in healthy elderly of Mexico City ABCL
Sánchez-Rodríguez,Martha A.; Retana-Ugalde,Raquel; Ruiz-Ramos,Mirna; Mendoza-Núñez,Víctor Manuel.
The aging is one of the factors that cause decrease in the antioxidant capacity. Likewise, it has been proposed that subjects exposed permanently to air pollution develop deficient antioxidant capacity to oxidative stress (OxS). This study aimed to analyze the antioxidant capacity against elevated lipid peroxides in healthy elderly of Mexico City. 105 adults (44 ± 10.8 years) and 126 elderly subjects were studied (68 ± 7.1 years); residents of Mexico City (clinically healthy, non-smokers, non-vitamin supplement takers) who had lived in the city for >10 years. Plasma lipoperoxides (LPO), total antioxidant status (TAS), the activity of red blood cells superoxide dismutase (SOD), and plasma glutathione peroxidase (GPx), were studied in all subjects. LPO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adaptation to oxidative stress; Total antioxidant status; Elderly people; Pollution; Superoxide dismutase; Glutathione peroxidase.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572004000200007
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Effect of silymarin on sodium fluoride-induced toxicity and oxidative stress in rat cardiac tissues Anais da ABC (AABC)
Nabavi,Seyed M.; Nabavi,Seyed F.; Moghaddam,Akbar H.; Setzer,William N.; Mirzaei,Morteza.
This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Glutathione; Sodium fluoride; Superoxide dismutase; Silymarin; TBARS.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400026
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Plasma nitric oxide, endothelin-1, arginase and superoxide dismutase in the plasma and placentae from preeclamptic patients Anais da ABC (AABC)
Bernardi,Fabiana C.; Vuolo,Francieli; Petronilho,Fabricia; Michels,Monique; Ritter,Cristiane; Dal-Pizzol,Felipe.
The aim of this study was to determine parameters of NO metabolism in plasma and placenta of preeclamptic (PE) patients. It was conducted a case-control study at São José Hospital, Brazil. Thirty-three PE and 33 normotensive pregnant were included in the study. The diagnosis of PE was established in accordance with the definitions of American College of Obstetricians and Gynecologists. Peripheral venous blood and placenta samples were obtained at postpartum period. Plasma NO levels and SOD activity were significantly lower and endothelin-1 levels and arginase activity were significantly higher in PE women when compared to controls. None of the analyzed parameters were different in the placenta between groups. Our findings suggest that parameters associated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Preeclampsia; Superoxide dismutase; Arginase activity; Endothelin-1 levels; Nitrite/nitrate.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200713
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Superoxide dismutase and catalase: tissue activities and relation with age in the long-lived species Margaritifera margaritifera Biol. Res.
FERNÁNDEZ,CARLOS; SAN MIGUEL,EDUARDO; FERNÁNDEZ-BRIERA,ALMUDENA.
Free radicals are extremely reactive and produce damage and modify cell functions. Furthermore, superoxide dismutase and catalase are believed to play a key role in the enzymatic defence of the cells. Indeed, some authors have argued that reduced free-radical damage could explain increased longevity. Margaritifera margaritifera is one of the longest-lived animals in the world (up to 100-200 years). Furthermore, this organism may serve as a useful model for gerontologists interested in exploring the mechanisms that promote long life and the slowing of senescence. The present study estimated for the first time individual enzymatic activity for superoxide dismutase isozymes (Cu,Zn-SOD and Mn-SOD) and catalase in tissue preparations of gills, digestive glands...
Tipo: Journal article Palavras-chave: Margaritifera margaritifera; Free radicals; Aging; Superoxide dismutase; Catalase.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100006
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Effect of Thymol on Soybean Seeds Germination: Physiological and Biochemical Analysis BABT
Lima,Eduarda Lee Ferreira; Macedo,Willian Rodrigues; Silva,Geraldo Humberto.
Abstract Higher levels of reactive species of oxygen are harmful to plant tissues. This study evaluated the action of different doses of thymol on soybean seed germination, biometric analysis and enzymatic parameters; both involved in germination process. High doses of thymol affected the plantlet growth, but not hampered the germination.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascorbate peroxidase; Catalase; Superoxide dismutase; Glycine max L; Seed vigor.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100211
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Age-related changes and effects of regular low-intensity exercise on gait, balance, and oxidative biomarkers in the spinal cord of Wistar rats BJMBR
Silveira,E.M.S.; Kroth,A.; Santos,M.C.Q.; Silva,T.C.B.; Silveira,D.; Riffel,A.P.K.; Scheid,T.; Trapp,M.; Partata,W.A..
The present study aimed to analyze age-related changes to motor coordination, balance, spinal cord oxidative biomarkers in 3-, 6-, 18-, 24-, and 30-month-old rats. The effects of low-intensity exercise on these parameters were also analyzed in 6-, 18-, and 24-month-old rats. Body weight, blood glucose, total cholesterol, and high-density lipoprotein (HDL) cholesterol were assessed for all rats. The soleus muscle weight/body weight ratio was used to estimate skeletal muscle mass loss. Body weight increased until 24 months; only 30-month-old rats exhibited decreased blood glucose and increased total cholesterol and HDL cholesterol. The soleus muscle weight/body weight ratio increased until 18 months, followed by a small decrease in old rats. Exercise did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superoxide anion generation; Hydrogen peroxide; Total thiol content; Lipid hydroperoxide levels; Total antioxidant capacity; Superoxide dismutase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700608
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