|
|
|
| Registros recuperados: 8.909 | |
|
|
| Baeza,Carlos M.; Kottirsch,Gabriele; Espejo,Jaime; Reinoso,Rodrigo. |
| Se entregan datos citológicos del número diploide de cromosomas de 15 especies de la flora vascular de Chile. Se utiliza material de raíces provientes de plántulas y bulbos cultivados en invernaderos. Las plantas estudiadas corresponden a representantes de Alstroemeriaceae, Asteraceae, Gomortegaceae, Iridaceae, Lauraceae, Orchidaceae, Poaceae y Tecophilaeaceae |
| Tipo: Journal article |
Palavras-chave: Números cromosómicos; Flora vascular de Chile. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432001000200004 |
| |
|
|
| Badano,Ernesto I.; Schlumpberger,Boris O.. |
| Los objetivos de este estudio fueron analizar el sistema de cruzamiento y la eficiencia de la polinización en Trichocereus pasacana y sus efectos sobre la reproducción. Se trabajó sobre dos poblaciones del noroeste Argentino, una dentro de un área protegida (Valle de Tin Tin) y otra en un sitio con marcada actividad humana de agricultura y apicultura (Cachi Adentro). El sistema de cruzamiento se analizó mediante un experimento donde se evaluó autopolinización manual, polinización cruzada manual, polinización natural y autopolinización automática. Las variables de respuesta fueron producción de frutos, producción de semillas y capacidad germinativa. La producción de frutos permite sugerir que el sistema de cruzamiento de T. pasacana es xenogámico y que la... |
| Tipo: Journal article |
Palavras-chave: Polinización; Sistema de cruzamiento; Actaceae; Cactus columnares; Argentina. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432001000200002 |
| |
|
|
| Bianchinotti,María Virginia. |
| Se estudió la micobiota asociada con plantas sanas en pie de Geoffroea decorticans (Fabaceae) mediante muestreos estacionales durante un año. Se identificaron 51 micromicetes (en su mayoría celomicetes) asociados a la superficie de ramas y ritidoma troncal. En el otoño se registró un aumento de la diversidad específica para todos los estratos muestreados. Según el espectro de estrategias establecido se observó un aumento del componente exclusivo de la comunidad asociada al ritidoma. De acuerdo a las técnicas de análisis multivariado utilizadas para analizar la frecuencia de aparición de las especies ("clustering" y ACP) se encontraron diferencias en la micobiota de acuerdo al sustrato y a la posición de éste en el dosel |
| Tipo: Journal article |
Palavras-chave: Geoffroea decorticans; Fabaceae; Micromicetes; Ecología. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432001000100001 |
| |
|
|
| Telles,Guilherme P.; Braga,Marília D.V.; Dias,Zanoni; Tzy-Li,Lin; Quitzau,José A.A.; Silva,Felipe R. da; Meidanis,João. |
| The Sugarcane EST project (SUCEST) produced 291,904 expressed sequence tags (ESTs) in a consortium that involved 74 sequencing and data mining laboratories. We created a web site for this project that served as a meeting point for receiving, processing, analyzing, and providing services to help explore the sequence data. In this paper we describe the information pathway that we implemented to support this project and a brief explanation of the clustering procedure, which resulted in 43,141 clusters. |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100003 |
| |
|
|
| Rossi,Magdalena; Araujo,Paula Gonçalves; Van Sluys,Marie-Anne. |
| The sugarcane expressed sequence tag (SUCEST) project has produced a large number of cDNA sequences from several plant tissues submitted or not to different conditions of stress. In this paper we report the result of a search for transposable elements (TEs) revealing a surprising amount of expressed TEs homologues. Of the 260,781 sequences grouped in 81,223 fragment assembly program (Phrap) clusters, a total of 276 clones showed homology to previously reported TEs using a stringent cut-off value of e-50 or better. Homologous clones to Copia/Ty1 and Gypsy/Ty3 groups of long terminal repeat (LTR) retrotransposons were found but no non-LTR retroelements were identified. All major transposon families were represented in sugarcane including Activator (Ac),... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100020 |
| |
|
| |
|
|
| Costa,R.M.A.; Lima,W.C.; Vogel,C.I.G.; Berra,C.M.; Luche,D.D.; Medina-Silva,R.; Galhardo,R.S.; Menck,C.F.M.; Oliveira,V.R.. |
| There is much interest in the identification and characterization of genes involved in DNA repair because of their importance in the maintenance of the genome integrity. The high level of conservation of DNA repair genes means that these genetic elements may be used in phylogenetic studies as a source of information on the genetic origin and evolution of species. The mechanisms by which damaged DNA is repaired are well understood in bacteria, yeast and mammals, but much remains to be learned as regards plants. We identified genes involved in DNA repair mechanisms in sugarcane using a similarity search of the Brazilian Sugarcane Expressed Sequence Tag (SUCEST) database against known sequences deposited in other public databases (National Center of... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100018 |
| |
|
|
| Lima,W.C.; Medina-Silva,R.; Galhardo,R.S.; Menck,C.F.M.. |
| DNA repair pathways are necessary to maintain the proper genomic stability and ensure the survival of the organism, protecting it against the damaging effects of endogenous and exogenous agents. In this work, we made an analysis of the expression patterns of DNA repair-related genes in sugarcane, by determining the EST (expressed sequence tags) distribution in the different cDNA libraries of the SUCEST transcriptome project. Three different pathways - photoreactivation, base excision repair and nucleotide excision repair - were investigated by employing known DNA repair proteins as probes to identify homologous ESTs in sugarcane, by means of computer similarity search. The results showed that DNA repair genes may have differential expressions in tissues,... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100019 |
| |
|
|
| Carraro,Dirce Maria; Lambais,Marcio R.; Carrer,Helaine. |
| Sucrose non-fermenting-1-related protein kinases (SnRKs) may play a major role in regulating gene expression in plant cells. This family of regulatory proteins is represented by sucrose non-fermenting-1 (SNF1) protein kinase in Saccharomyces cerevisiae, AMP-activated protein kinases (AMPKs) in mammals and SnRKs in higher plants. The SnRK family has been reorganized into three subfamilies according to the evolutionary relationships of their amino acid sequences. Members of the SnRK subfamily have been identified in several plants. There is evidence that they are involved in the nutritional and/or environmental stress response, although their roles are not yet well understood. We have identified at least 22 sugarcane expressed sequence tag (EST) contigs... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100006 |
| |
|
|
| Andrietta,Mírian Helene; Eloy,Núbia Barbosa; Hemerly,Adriana Silva; Ferreira,Paulo C.G.. |
| Data on cell cycle research in plants indicate that the majority of the fundamental regulators are conserved with other eukaryotes, but the controlling mechanisms imposed on them, and their integration into growth and development is unique to plants. To date, most studies on cell division have been conducted in dicot plants. However, monocot plants have distinct developmental strategies that will affect the regulation of cell division at the meristems. In order to advance our understanding how cell division is integrated with the basic mechanisms controlling cell growth and development in monocots, we took advantage of the sugarcane EST Project (Sucest) to carry an exhaustive data mining to identify components of the cell cycle machinery. Results obtained... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100010 |
| |
|
|
| Vettore,André L.; Silva,Felipe R. da; Kemper,Edson L.; Arruda,Paulo. |
| A large-scale sequencing of sugarcane expressed sequence tags (ESTs) was carried out as a first step in depicting the genome of this important tropical crop. Twenty-six unidirectional cDNA libraries were constructed from a variety of tissues sampled from thirteen different sugarcane cultivars. A total of 291,689 cDNA clones were sequenced in their 5 and 3end regions. After trimming low-quality sequences and removing vector and ribosomal RNA sequences, 237,954 ESTs potentially derived from protein-encoding messenger RNA (mRNA) remained. The average insert size in all libraries was estimated to be 1,250bp with the insert length varying from 500 to 5,000 bp. Clustering the 237,954 sugarcane ESTs resulted in 43,141clusters, from which 38% had no matches with... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100002 |
| |
|
|
| Bacci Jr.,M.; Miranda,V.F.O.; Martins,V.G.; Figueira,A.V.O.; Lemos,M.V.; Pereira,J.O.; Marino,C.L.. |
| To determine the phylogenetic relationship between sugarcane cultivars and other members of the Saccharinae subtribe, we identified the fast evolving ITS1-5.8S-ITS2 (ITS = internal transcribed spacer; 5.8S = 5.8S ribosomal DNA) region of the sugarcane genome in the Sugarcane Expressed Sequence Tag (SUCEST) genome project database. Parsimony analysis utilizing this region and homologs belonging to the 23 closely related Andropogoneae currently deposited in the GenBank database has shown sugarcane as the sister group of Saccharum sinense. However, because there are few parsimony-informative characters and high homoplasy in the ITS1-5.8S-ITS2 region we were not able to determine with confidence the phylogenetic relationship between sugarcane and some of the... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100023 |
| |
|
| |
|
|
| Marbach,Phellippe A. Santos; Coelho,Alexandre S. Guedes; Silva-Filho,Marcio C.. |
| A phylogenetic analysis of plant FtsH-like proteins was performed using protein sequences from the GENEBANK database and five groups of plant FtsH-like proteins were identified by neighbor-joining analysis. Prediction of the subcellular location of the proteins suggested that two (FtsH-m1 & FtsH-m2) were mitochondrial and three (FtsH-p1, FtsH-p2, FtsH-p3) were plastid targeting. The phylogenetic profile of plant FtsH-like proteins was used to search sugarcane expressed sequence tag (EST) clusters in the SUCEST database. Initially, 153 clusters presenting homology with FtsH-like proteins were recovered, of which 23 were confirmed by a BLAST search in the GENEBANK database and by comparison of their hidropathy index with that of previously described... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100025 |
| |
|
|
| Correa,Gustavo Coelho; Margis-Pinheiro,Márcia; Margis,Rogério. |
| Cysteine proteases are peptidyl hydrolyses dependent on a cysteine residue at the active center. The physical and chemical properties of cysteine proteases have been extensively characterized, but their precise biological functions have not yet been completely understood, although it is known that they are involved in a number of events such as protein turnover, cancer, germination, programmed cell death and senescence. Protein sequences from different cysteine proteinases, classified as members of the E.C.3.4.22 sub-sub-class, were used to perform a T-BLAST-n search on the Brazilian Sugarcane Expressed Sequence Tags project (SUCEST) data bank. Sequence homology was found with 76 cluster sequences that corresponded to possible cysteine proteinases. The... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100036 |
| |
|
| |
|
| |
|
|
| Silva,Jorge A.G. da. |
| Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight sugarcane genotypes, the sequences of these primers and a list of known sugarcane genes containing SSR motifs being presented in this paper. Polymorphisms were evident both at the cultivar level and between Saccharum species. These results show that EST-derived SSRs in Saccharum species are useful because they are polymorphic and transferable. The large number of... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100021 |
| |
|
|
| Lambais,Marcio R.. |
| The expression patterns of 277 sugarcane expressed sequence tags (EST)-contigs encoding putative defense-related (DR) proteins were evaluated using the Sugarcane EST database. The DR proteins evaluated included chitinases, beta-1,3-glucanases, phenylalanine ammonia-lyases, chalcone synthases, chalcone isomerases, isoflavone reductases, hydroxyproline-rich glycoproteins, proline-rich glycoproteins, peroxidases, catalases, superoxide dismutases, WRKY-like transcription factors and proteins involved in cell death control. Putative sugarcane WRKY proteins were compared and their phylogenetic relationships determined. A hierarchical clustering approach was used to identify DR ESTs with similar expression profiles in representative cDNA libraries. To identify DR... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100015 |
| |
| Registros recuperados: 8.909 | |
|
|
|
