Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 14.614
Primeira ... 91011121314151617 ... Última
Imagem não selecionada

Imprime registro no formato completo
Deletion of DNA sequences of using a polymerase chain reaction based approach Electron. J. Biotechnol.
Pérez-Pinera,Pablo; Menéndez-González,Manuel; Vega,José Antonio.
We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a restriction enzyme target sequence. Following PCR amplification, the plasmid is digested with Dpn I to eliminate the template DNA, with the chosen restriction enzyme, and ligated. The only limitation is the selection of the restriction enzyme target sequence that must not be present in the original plasmid. The method is straightforward in its execution and success relies on a meticulous primer design that permits us obtain 100% of transformants containing the desired...
Tipo: Journal article Palavras-chave: Deletion; Mutagenesis; Polymerase chain reaction; Plasmid.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500018
Imagem não selecionada

Imprime registro no formato completo
Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture Electron. J. Biotechnol.
Batista,Fabiana R. X.; Pereira,Carlos A.; Mendonça,Ronaldo Z.; Moraes,Angela M..
Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filtration retentate (MWR) when associated to yeast extract (YE), glucose and Pluronic F68 (PF68) to partially replace fetal calf serum (FBS) in Spodoptera frugiperda Sf9 cells culture in Grace's medium, aiming AgMNPV baculovirus production. Batch cultivation results showed that the yeast extract and the milk whey concentrate effectively increased cell concentration to about half the level commonly verified for the Sf900II serum-free medium. A 4-fold increase in...
Tipo: Journal article Palavras-chave: Baculovirus; Concentrated milk whey; Grace's medium; Insect cells; Media formulation; Spodoptera frugiperda Sf9.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500007
Imagem não selecionada

Imprime registro no formato completo
Frequency of transferable multiple antibiotic resistance amongst coliform bacteria isolated from a treated sewage effluent in Antofagasta, Chile Electron. J. Biotechnol.
Silva,Juan; Castillo,Gabriela; Callejas,Lorena; López,Héctor; Olmos,Janet.
Antibiotic-resistant coliform bacteria from raw and treated water from activated sludge of a sewage treatment plant were isolated on eosin methylene blue agar (EMB) plates in the separate presence of ampicillin, tetracycline, chloramphenicol and streptomycin. Antibiotic resistant coliforms obtained from inflow and effluent waters were studied to determine influences of treatment on the species composition, resistance patterns and transferable resistance in these bacteria. The bacterial counts obtained showed a high proportion of antibiotic resistant coliforms in the treated effluent. The resistant coliform species were eliminated at different rates by the treatment. Escherichia coli decreased, whereas the proportion of Klebsiella sp increased. The...
Tipo: Journal article Palavras-chave: Antibiotic resistance; Coliforms; Plasmid; Sewage treatment; Water pollution.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500008
Imagem não selecionada

Imprime registro no formato completo
Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. Electron. J. Biotechnol.
Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Gokare Aswathanarayana,Ravishankar.
Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 µM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by...
Tipo: Journal article Palavras-chave: Aspergillus; Calcium; Culture filtrate; Dried cell powder; Elicitor; Glutathione; Methyljasmonate; Rhizophus; Thidiazuron.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500006
Imagem não selecionada

Imprime registro no formato completo
A new method: measurement of microleakage volume using human, dog and bovine permanent teeth Electron. J. Biotechnol.
Yavuz,Izzet; Aydin,Haluk; Ulku,Refik; Kaya,Sadullah; Tumen,Caner.
This study investigates the feasibility of a different new approach to determining the microleakage volume associated with dental restorations (Class V cavity restorated with glass ionomer cement + high copper amalgam) and the relative marginal adaptation deficiency of dog, bovine and human permanent teeth in in vitro conditions. Also researched is the appropriateness of using dog and bovine teeth in in vitro studies rather than human teeth. Our method utilizes the molecular adsorption characteristics of methylene blue. Within the framework of this study, 60 permanent teeth (20 human, 20 dogs and 20 bovine) were used. These groups were evaluated statistically, of which indicated no statistically significant differences (p > 0.05). It was also concluded...
Tipo: Journal article Palavras-chave: Dye adsorption test; Microleakage; Tooth.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100003
Imagem não selecionada

Imprime registro no formato completo
Resistance to freezing in three Eucalyptus globulus Labill subspecies Electron. J. Biotechnol.
Moraga S.,Priscila; Escobar,René; Valenzuela A.,Sofía.
The resistance to low temperatures was assessed in seedlings of three subspecies of E. globulus (ssp globulus, ssp bicostata and ssp maidenii) of two different provenances. Lethal temperature 50 (LT50) was obtained by measuring the electrolytic conductivity, nucleation and freezing temperatures were obtained by thermal analysis and the total soluble carbohydrates concentration was determined through the phenol-sulphuric method. Results showed that the most resistant provenance corresponded to Bolaro Mountain of ssp maidenii with a LT50 lower than -9ºC. The provenance Moogora of ssp globulus, had a LT50 of -8.47ºC, which situates it, as the second most resistant to low temperatures. According to the nucleation and freezing temperatures,...
Tipo: Journal article Palavras-chave: Cold resistance; Ssp bicostata; Ssp globulus; Ssp maidenii.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300026
Imagem não selecionada

Imprime registro no formato completo
Biotransformation of anthracene and fluoranthene by Absidia fusca Linnemann Electron. J. Biotechnol.
Villemain,Danièle; Guiraud,Pascale; Bordjiba,Ouahiba; Steiman,Régine.
A strain of Absidia fusca was isolated from a pesticide-contaminated soil (Annaba, Algeria). The biotransformation capability of this strain towards two polycyclic aromatic hydrocarbons (PAHs): anthracene and fluoranthene was compared to that exhibited by another strain of A. fusca isolated from a non-contaminated milieu and considered as a control. The results obtained were statistically analyzed and showed that the strain isolated from the contaminated soil was more efficient than the control to remove anthracene from the medium, during all the kinetics (90% removed versus 45% after 24 hrs). Concerning fluoranthene, the amount removed by both strains was very high during the first 24 hrs however the control strain was slightly more efficient (94% versus...
Tipo: Journal article Palavras-chave: Absidia fusca; Anthracene; Biodegradation; Fluoranthene; Kinetics.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200004
Imagem não selecionada

Imprime registro no formato completo
Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis Electron. J. Biotechnol.
Rakariyatham,Nuansri; Butr-Indr,Bordin; Niamsup,Hataichanoke; Shank,Lalida.
A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Its myrosinase production was selected on low cost medium prepared from mustard seed cake (Brassica juncea). Studies of production and stability of the enzyme showed that EMS mutagenesis increased myrosinase activity. Aspergillus sp. NR4617E1 produced myrosinase 1.90 U ml-1 at 36 hrs of the cultivation equivalent to 171% of the enzyme production in wild-type. The stability studies revealed that myrosinase from the mutant strains retained...
Tipo: Journal article Palavras-chave: Allylisothiocyanate; Aspergillus sp.; B-thioglucosidase; Myrosinase; EMS mutagenesis; MNNG mutagenesis.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400005
Imagem não selecionada

Imprime registro no formato completo
Genetic engineering applications in animal breeding Electron. J. Biotechnol.
Montaldo,Hugo H..
This paper discusses the use of genetic engineering applications in animal breeding, including a description of the methods, their potential and current uses and ethical issues. Genetic engineering is the name of a group of techniques used to identify, replicate, modify and transfer the genetic material of cells, tissues or complete organisms. Important applications of genetic engineering in animal breeding are: 1) Marker-assisted selection (MAS). The objective of this technology is to increase disease resistance, productivity and product quality in economically important animals by adding information of DNA markers to phenotypes and genealogies for selection decisions. 2) Transgenesis, the direct transfer of specific genes/alleles between individuals,...
Tipo: Journal article Palavras-chave: Adult mammalian cloning; Biotechnology; Gene mapping; GMOS; MAS; QTL; Transgenics.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200010
Imagem não selecionada

Imprime registro no formato completo
Antimicrobial effect of polyphenols from apple skins on human bacterial pathogens Electron. J. Biotechnol.
Alberto,María Rosa; Rinsdahl Canavosio,Matías Andrés; Manca de Nadra,María Cristina.
Apples possess many beneficial properties for the human health related with their high content in phenolic compounds. The antimicrobial effect of these compounds from the skin of two apple varieties, Royal Gala and Granny Smith, against human pathogens was examined. The phenolic compounds were extracted with the following solvents: A, acetone: water: acetic acid; B, ethyl acetate: methanol: water and C, ethanol: water. Total phenolic, flavonoid and non-flavonoid contents were analyzed in the extracts. The antimicrobial effect was determined using the agar diffusion method. The highest inhibitory effect of both apple varieties corresponded to extract A, which contained a high phenolic content. The Granny Smith extracts with higher phenolic content presented...
Tipo: Journal article Palavras-chave: Bacterial inhibition; Granny Smith; Royal Gala; Pathogens; Phenolic extracts.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300006
Imagem não selecionada

Imprime registro no formato completo
Establishment of an in vitro micropropagation protocol for Mecardonia tenella Electron. J. Biotechnol.
Alderete,Liliana Marisol; Mori,Marcela; Kato,Adriana; Salvio Escandón,Alejandro.
Mecardonia tenella is an attractive herbaceous native plant, characterized by their intense green foliage and their abundant yellow flowers. It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. The goal of the present paper is to study the in vitro behaviour of M. tenella and to establish a routine protocol for its micropropagation. For the in vitro establishment of M. tenella, nodal segments were disinfected by standard methods using ethanol/sodium hypochloride and cultured on hormone-free MS medium, supplemented with a mixture of antibiotics and an antifungal. In order to study the hormonal requirements of the species, nodal segments were cultured on basal MS supplemented with...
Tipo: Journal article Palavras-chave: In vitro; Micropropagation; Scrophulariaceae.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300017
Imagem não selecionada

Imprime registro no formato completo
Effect of different metals on protease activity in sunflower cotyledons Electron. J. Biotechnol.
Pena,Liliana B.; Tomaro,María L.; Gallego,Susana M..
Tipo: Journal article
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300016
Imagem não selecionada

Imprime registro no formato completo
Nutrient enrichment of cassava peels using a mixed culture of Saccharomyces cerevisae and Lactobacillus spp solid media fermentation techniques Electron. J. Biotechnol.
Oboh,Ganiyu.
Cassava pulp was fermented with pure strains of Saccharomyces cerevisae and two bacteria namely Lactobacillus delbruckii and Lactobacillus coryneformis for 3 days. The squeezed liquid from the fermented pulp was used to ferment cassava peels for 7 days. Analysis of the dried fermented peels revealed that there was a significant (P < 0.05) increase in the protein content of the cassava peels fermented with squeezed liquid from the inoculated cassava pulp (21.5%) when compared with the unfermented cassava peel (8.2%). Moreover, the treatment equally brought about a significant (P < 0.05) decrease in the cyanide (6.2 mg/kg) and phytate content (789.7 mg/100g) when compared with the unfermented cassava peels, which had 44.6 mg/kg cyanide and 1043.6...
Tipo: Journal article Palavras-chave: Cassava peels; Cyanide; Fermentatio; Protein.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100007
Imagem não selecionada

Imprime registro no formato completo
Lactic acid production by a strain of Lactococcus lactis subs lactis isolated from sugar cane plants Electron. J. Biotechnol.
Serna Cock,Liliana; Rodríguez de Stouvenel,Aida.
A Lactococcus lactis subs lactis strain was selected from 20 lactic acid strains isolated from the leaves of sugar cane plants, variety CC 85-92. The effects of substrate concentration and pH control on lactic acid (LA) production, glucose conversion (GC) and yield (Yp/s) were investigated using this strain. In batch fermentation at 32&ordm;C, with 60 gl-1 of glucose and a pH of 6.0, concentrations of up to 35 gl-1 of lactic acid were obtained. Maximum production and glucose conversion was achieved at low glucose concentrations. The strain shows great potential for lactic acid production from glucose, even without using pH control during fermentation (13.7 gl-1)
Tipo: Journal article Palavras-chave: CC 85-92; Leaves of sugar cane; PH control; Substrate concentration.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100006
Imagem não selecionada

Imprime registro no formato completo
Cotton genetic diversity study by AFLP markers Electron. J. Biotechnol.
Murtaza,Naveed.
Amplified fragmentlength polymorphism (AFLP) markers have been used to ascertain the intensity of inherent diversity and relatedness in cotton (Gossypium spp.) plants. The effectiveness of this method to distinguish inter and intra specific difference in cotton could be handy in cultivar recognition and in marker assisted parental selection tool for plant breeders. Twenty cotton cultivars belonging to Gossypium hirsutum L., and G. arborium L. from the Pakistan and US origin were used for AFLP based genetic diversity estimates. The objective of this study was to assess the level of genetic variation among some cotton cultivars belonging to the old and new world species of cotton. Four EcoRI-MseI primer-pair combinations were used forthe AFLP analysis. The...
Tipo: Journal article Palavras-chave: AFLP; Cluster analysis; Cotton; Genetic diversity; PCR.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400013
Imagem não selecionada

Imprime registro no formato completo
Yeast lipases: enzyme purification, biochemical properties and gene cloning Electron. J. Biotechnol.
Vakhlu,Jyoti.
Lipases are placed only after proteases and carbohydrases in world enzyme market and share about 5% of enzyme market. They occur in plants, animals and microorganisms and are accordingly classified as plant, animal and microbial lipases. Wherever they exist, they function to catalyze hydrolysis of triglycerides to glycerol and fatty acid. Like carbohydrases and proteases, lipases of microbial origin enjoy greater industrial importance as they are more stable (compared to plant and animal lipases) and can be obtained in bulk at low cost. Majority of yeast lipases are extracelluar, monomericglycoproteins with molecular weight ranging between ~33 to ~65 kD. More than 50% reported lipases producing yeast, produce it in the forms of various isozymes. These...
Tipo: Journal article Palavras-chave: Candida; Geotrichum; Lipase; Trichosporon; Yeast.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100010
Imagem não selecionada

Imprime registro no formato completo
Immobilization and stability studies of a lipase from thermophilic Bacillus sp: The effect of process parameters on immobilization of enzyme Electron. J. Biotechnol.
Nawani,Neerupma; Singh,Rajvinder; Kaur,Jagdeep.
A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60&ordm;C and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The catalytic properties of this thermostable enzyme can be further improved via the use of different immobilization techniques and reaction conditions. Enzyme was immobilized on different solid supports and their enzyme activity and stability was compared. The enzyme was adsorbed on silica and HP-20 beads followed by cross-linking with gluteraldehyde on HP-20,...
Tipo: Journal article Palavras-chave: Esterification; Immobilization; Lipase; Thermostability.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500011
Imagem não selecionada

Imprime registro no formato completo
Management of the appropriate agricultural biotechnology for small producers: Bolivia case study Electron. J. Biotechnol.
Avila,Teresa; Izquierdo,Juan.
The Bolivia study is part of an ongoing multiple case study organized by the FAO's Regional Office for Latin America and the Caribbean as part of the activities of the Technical Cooperation Network on Agricultural Biotechnology (REDBIO/FAO). The basic information was obtained through a survey and/or direct interview to a significant number of experts from Bolivian institutions involving management, regulation, research, innovation, application and education of agricultural biotechnology. At national level, the most relevant applied agri-biotechnologies are presently in vitro plant culture, genomics, molecular genetics, assisted reproduction techniques in animals, and techniques supporting plant and animal disease diagnosis. They have been developed and...
Tipo: Journal article Palavras-chave: Andean crops; Appropriate biotechnology; Biotechnology administration; Bolivia; Genetic resources; Policy; REDBIO/FAO; Regulations; Technical cooperation network on plant biotechnology.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100002
Imagem não selecionada

Imprime registro no formato completo
Biodegradation of agroindustrial wastes by Pleurotus spp for its use as ruminant feed Electron. J. Biotechnol.
Alborés,Silvana; Pianzzola,María Julia; Soubes,Matilde; Cerdeiras,María Pía.
The increasing expansion of agro-industrial activity has led to the accumulation of a large quantity of lignocellulosic residues all over the world. In particular, large quantities of rice straw (300.000 t) and citric bagasse (50.000 t) are annually produced in Uruguay. In this work we present the study of the bioconversion of these substrates with the edible mushroom Pleurotus spp so as to increase nutritional values and digestibility for its use as animal feed. The SSF process was optimized and the products after different periods of mushroom growth were evaluated. The microbial counts (cfu/g) for the inoculated substrates 44 days after incubation were 15 x 10(4), < 10 and < 10 for aerobic microorganisms, coliforms and E. coli, respectively. After...
Tipo: Journal article Palavras-chave: Agroindustry; Basidiomycetes; Ruminant feed.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300008
Imagem não selecionada

Imprime registro no formato completo
Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon Electron. J. Biotechnol.
Siciliano,Florencia; Torres,Pablo; Sendín,Lorena; Bermejo,Carolina; Filippone,Paula; Vellice,Gabriel; Ramallo,Jackie; Castagnaro,Atilio; Vojnov,Adrian; Marano,María Rosa.
Xanthomonas axonopodis pathovar citri (Xac) causes bacterial citrus canker, a serious disease of most citrus species. Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. In Xcc, cell-cell signaling is mediated by diffusible signal factor (DSF). Synthesis of DSF depends on RpfB and RpfF. DSF perception and signal transduction have been suggested to involve a two-component system comprising RpfC and RpfG. It has been proposed that these proteins participate in a signal transduction system linking changes in the environment to the synthesis of DSF and the expression of virulence genes. Although the cluster of the rpf genes in Xac has synteny with the corresponding cluster in Xcc, two genes (rpfH and...
Tipo: Journal article Palavras-chave: Canker; DSF; Extracellular polysaccharide; Quorum sensing.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300005
Registros recuperados: 14.614
Primeira ... 91011121314151617 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional