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Registros recuperados: 14.614 | |
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Yavuz,Izzet; Aydin,Haluk; Ulku,Refik; Kaya,Sadullah; Tumen,Caner. |
This study investigates the feasibility of a different new approach to determining the microleakage volume associated with dental restorations (Class V cavity restorated with glass ionomer cement + high copper amalgam) and the relative marginal adaptation deficiency of dog, bovine and human permanent teeth in in vitro conditions. Also researched is the appropriateness of using dog and bovine teeth in in vitro studies rather than human teeth. Our method utilizes the molecular adsorption characteristics of methylene blue. Within the framework of this study, 60 permanent teeth (20 human, 20 dogs and 20 bovine) were used. These groups were evaluated statistically, of which indicated no statistically significant differences (p > 0.05). It was also concluded... |
Tipo: Journal article |
Palavras-chave: Dye adsorption test; Microleakage; Tooth. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100003 |
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Moraga S.,Priscila; Escobar,René; Valenzuela A.,Sofía. |
The resistance to low temperatures was assessed in seedlings of three subspecies of E. globulus (ssp globulus, ssp bicostata and ssp maidenii) of two different provenances. Lethal temperature 50 (LT50) was obtained by measuring the electrolytic conductivity, nucleation and freezing temperatures were obtained by thermal analysis and the total soluble carbohydrates concentration was determined through the phenol-sulphuric method. Results showed that the most resistant provenance corresponded to Bolaro Mountain of ssp maidenii with a LT50 lower than -9ºC. The provenance Moogora of ssp globulus, had a LT50 of -8.47ºC, which situates it, as the second most resistant to low temperatures. According to the nucleation and freezing temperatures,... |
Tipo: Journal article |
Palavras-chave: Cold resistance; Ssp bicostata; Ssp globulus; Ssp maidenii. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300026 |
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Montaldo,Hugo H.. |
This paper discusses the use of genetic engineering applications in animal breeding, including a description of the methods, their potential and current uses and ethical issues. Genetic engineering is the name of a group of techniques used to identify, replicate, modify and transfer the genetic material of cells, tissues or complete organisms. Important applications of genetic engineering in animal breeding are: 1) Marker-assisted selection (MAS). The objective of this technology is to increase disease resistance, productivity and product quality in economically important animals by adding information of DNA markers to phenotypes and genealogies for selection decisions. 2) Transgenesis, the direct transfer of specific genes/alleles between individuals,... |
Tipo: Journal article |
Palavras-chave: Adult mammalian cloning; Biotechnology; Gene mapping; GMOS; MAS; QTL; Transgenics. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200010 |
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Alberto,María Rosa; Rinsdahl Canavosio,Matías Andrés; Manca de Nadra,María Cristina. |
Apples possess many beneficial properties for the human health related with their high content in phenolic compounds. The antimicrobial effect of these compounds from the skin of two apple varieties, Royal Gala and Granny Smith, against human pathogens was examined. The phenolic compounds were extracted with the following solvents: A, acetone: water: acetic acid; B, ethyl acetate: methanol: water and C, ethanol: water. Total phenolic, flavonoid and non-flavonoid contents were analyzed in the extracts. The antimicrobial effect was determined using the agar diffusion method. The highest inhibitory effect of both apple varieties corresponded to extract A, which contained a high phenolic content. The Granny Smith extracts with higher phenolic content presented... |
Tipo: Journal article |
Palavras-chave: Bacterial inhibition; Granny Smith; Royal Gala; Pathogens; Phenolic extracts. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300006 |
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Oboh,Ganiyu. |
Cassava pulp was fermented with pure strains of Saccharomyces cerevisae and two bacteria namely Lactobacillus delbruckii and Lactobacillus coryneformis for 3 days. The squeezed liquid from the fermented pulp was used to ferment cassava peels for 7 days. Analysis of the dried fermented peels revealed that there was a significant (P < 0.05) increase in the protein content of the cassava peels fermented with squeezed liquid from the inoculated cassava pulp (21.5%) when compared with the unfermented cassava peel (8.2%). Moreover, the treatment equally brought about a significant (P < 0.05) decrease in the cyanide (6.2 mg/kg) and phytate content (789.7 mg/100g) when compared with the unfermented cassava peels, which had 44.6 mg/kg cyanide and 1043.6... |
Tipo: Journal article |
Palavras-chave: Cassava peels; Cyanide; Fermentatio; Protein. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100007 |
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Murtaza,Naveed. |
Amplified fragmentlength polymorphism (AFLP) markers have been used to ascertain the intensity of inherent diversity and relatedness in cotton (Gossypium spp.) plants. The effectiveness of this method to distinguish inter and intra specific difference in cotton could be handy in cultivar recognition and in marker assisted parental selection tool for plant breeders. Twenty cotton cultivars belonging to Gossypium hirsutum L., and G. arborium L. from the Pakistan and US origin were used for AFLP based genetic diversity estimates. The objective of this study was to assess the level of genetic variation among some cotton cultivars belonging to the old and new world species of cotton. Four EcoRI-MseI primer-pair combinations were used forthe AFLP analysis. The... |
Tipo: Journal article |
Palavras-chave: AFLP; Cluster analysis; Cotton; Genetic diversity; PCR. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400013 |
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Vakhlu,Jyoti. |
Lipases are placed only after proteases and carbohydrases in world enzyme market and share about 5% of enzyme market. They occur in plants, animals and microorganisms and are accordingly classified as plant, animal and microbial lipases. Wherever they exist, they function to catalyze hydrolysis of triglycerides to glycerol and fatty acid. Like carbohydrases and proteases, lipases of microbial origin enjoy greater industrial importance as they are more stable (compared to plant and animal lipases) and can be obtained in bulk at low cost. Majority of yeast lipases are extracelluar, monomericglycoproteins with molecular weight ranging between ~33 to ~65 kD. More than 50% reported lipases producing yeast, produce it in the forms of various isozymes. These... |
Tipo: Journal article |
Palavras-chave: Candida; Geotrichum; Lipase; Trichosporon; Yeast. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100010 |
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Nawani,Neerupma; Singh,Rajvinder; Kaur,Jagdeep. |
A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60ºC and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The catalytic properties of this thermostable enzyme can be further improved via the use of different immobilization techniques and reaction conditions. Enzyme was immobilized on different solid supports and their enzyme activity and stability was compared. The enzyme was adsorbed on silica and HP-20 beads followed by cross-linking with gluteraldehyde on HP-20,... |
Tipo: Journal article |
Palavras-chave: Esterification; Immobilization; Lipase; Thermostability. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500011 |
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Avila,Teresa; Izquierdo,Juan. |
The Bolivia study is part of an ongoing multiple case study organized by the FAO's Regional Office for Latin America and the Caribbean as part of the activities of the Technical Cooperation Network on Agricultural Biotechnology (REDBIO/FAO). The basic information was obtained through a survey and/or direct interview to a significant number of experts from Bolivian institutions involving management, regulation, research, innovation, application and education of agricultural biotechnology. At national level, the most relevant applied agri-biotechnologies are presently in vitro plant culture, genomics, molecular genetics, assisted reproduction techniques in animals, and techniques supporting plant and animal disease diagnosis. They have been developed and... |
Tipo: Journal article |
Palavras-chave: Andean crops; Appropriate biotechnology; Biotechnology administration; Bolivia; Genetic resources; Policy; REDBIO/FAO; Regulations; Technical cooperation network on plant biotechnology. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100002 |
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Siciliano,Florencia; Torres,Pablo; Sendín,Lorena; Bermejo,Carolina; Filippone,Paula; Vellice,Gabriel; Ramallo,Jackie; Castagnaro,Atilio; Vojnov,Adrian; Marano,María Rosa. |
Xanthomonas axonopodis pathovar citri (Xac) causes bacterial citrus canker, a serious disease of most citrus species. Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. In Xcc, cell-cell signaling is mediated by diffusible signal factor (DSF). Synthesis of DSF depends on RpfB and RpfF. DSF perception and signal transduction have been suggested to involve a two-component system comprising RpfC and RpfG. It has been proposed that these proteins participate in a signal transduction system linking changes in the environment to the synthesis of DSF and the expression of virulence genes. Although the cluster of the rpf genes in Xac has synteny with the corresponding cluster in Xcc, two genes (rpfH and... |
Tipo: Journal article |
Palavras-chave: Canker; DSF; Extracellular polysaccharide; Quorum sensing. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300005 |
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Registros recuperados: 14.614 | |
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