|
|
|
| Registros recuperados: 77 | |
|
|
| 猪熊, 壽; 堀内, 雅之; 古林, 与志安; INOKUMA, Hisashi; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu. |
| 臨床的に牛白血病を疑ったものの，牛白血病ではないと確定診断されたホルスタイン種乳牛11症例の臨床所見を回顧的に整理した．11症例は体表リンパ節腫大あるいは体腔内の腫瘤触知により牛白血病との鑑別診断が必要であったが，病理解剖により乳房炎，子宮蓄膿症，膿瘍，肺炎，腸炎またはアミロイド症と診断された．牛白血病ウイルス（Bovine leukemia virus: BLV）抗体陽性を呈した4頭のうち，リンパ球増多症を呈したものが2頭であった．またこれら2頭のうち1頭はリンパ節細胞診でも大型リンパ球優勢であった．しかし，血清チミジンキナーゼ（Tymidine kinase: TK），総乳酸脱水素酵素（Lactate dehydrogenase: LDH），LDHアイソザイム2および3の活性値は4頭とも基準値以内であり，牛白血病発症は否定的であった．BLV抗体陰性牛7頭のうち，リンパ節細胞診を実施した2頭は小型リンパ球優勢であり，また，リンパ球増多症を呈した症例はいなかった．1症例では血清TK活性値が基準値をわずかに超えており，また，他の2頭は総LDH，LDHアイソザイム2および3の活性値がいずれも基準値を超えていたが，他の検査項目は牛白血病について否定的であった．腫大リンパ節の細胞診，リンパ球数算定，血清TK，総LDH，LDHアイソザイム2および3の活性値測定といった牛白血病診断のための検査を複数実施することで，牛白血病を否定するための診断精度を高めることが可能と考えられた． |
|
Palavras-chave: 牛白血病; 鑑別診断; 血清チミジンキナーゼ; LDH; LDHアイソザイム. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4367 |
| |
|
|
| 久保田, 直樹; 坂田, 義美; 宮崎, 直美; 板本, 和仁; 坂内, 天; 西川, 義文; 玄, 学南; 猪熊, 壽; NISHIKAWA, Yoshifumi; XUAN, Xuenan; INOKUMA, Hisashi. |
| 組換抗原を用いた種特異的ELISAにより,犬のNeospora caninum感染について全国的血清疫学調査を実施した.1,206頭中全国30県から126頭(10.4%)が陽性を示した.陽性率は,雄よりも雌で有意に高く,また他品種と比較して有意に高い陽性率を示す品種がみられた.子宮蓄膿症および糖尿病の病歴を有する犬の陽性率は,他の疾病を有する犬または疾病のない犬の陽性率に比べて高値であった. A seroepidemiological survey of Neospora caninum infection among dogs in Japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (Nc-SAG1t). Among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from Hokkaido to Okinawa were positive to N. caninum infections, which were more frequently detected in females than males. Siberian Huskies showed the highest positive rate compared with the other breeds. Dogs with pyometra and diabetes mellitus showed the higher positive rates than dogs with other... |
|
Palavras-chave: Canine; Nbospora caninum; Species-specific ELISA. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4003 |
| |
|
|
| 坂田, 義美; 市川, 康明; 猪熊, 壽; SAKATA, Yoshimi; ICHIKAWA, Yasuaki; INOKUMA, Hisashi. |
| 犬のE.canisおよびA.ρhagocytophilum感染状況を明らかにするため，従来法である蛍光抗体法(IFA)，および組換え主要膜表面蛋白質を抗原とした酵素抗体法を原理とする新規の特異抗体検出キットを組合せて全国的血清疫学調査を行った. IFAによるスクリーニング検査では1,206検体中64頭(5.3%)がE.canis 20倍以上の抗体価を示したが，これら64頭は特異抗体検出キットではすべてE.canis陰性であり，E. canis以外の近縁種に感染していたと考えられた.いっぽうIFAでA.ρhagocytophilumに対して20倍以上の抗体価を示したものは1,206検体中3頭(0.2%)であった.うち1頭は特異抗体検出キットでもA.phagocytophilum陽性を示し，日本にもA.phagocytophilumに感染した犬 がいることが示唆された.しかし，抗体陽性犬に臨床症状は認められず，病原性との関連は不明であった. A seroepidemiological survey of Ehrlichia canis and Anaplasma phagocytophilum infection in dogs in Japan was performed by using a combination of a classical immunofluorescence antibody assay (IFA) and a new enzyme immune assay (EIA) kit that uses a specific recombinant major surface protein of the pathogens. Although 64 (5.3%) of 1,206... |
|
Palavras-chave: 犬; エールリッヒア; アナプラズマ; 血清疫学調査; Anaplasma phagocytophilum; Dog; Ehrlichia canis. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2818 |
| |
|
|
| YBAÑEZ, Adrian Patalinghug; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular evidence for the presence of these 2 species in Japan, and to develop a reliable PCR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5% (50 samples). The... |
|
Palavras-chave: 16S rRNA; Anaplasma phagocytophilum; Anaplasma sp.; GltA; GroEL. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3839 |
| |
|
|
| 猪熊, 壽; 永田, 美和; 細井, 栄嗣; 板本, 和仁; 奥田, 優; INOKUMA, Hisashi. |
| 全国8地域のニホンジカに寄生するタイレリアの18S rRNAおよびp33/34遺伝子を解析した. 18S rRNA遺伝子の相同性は99.7-100%であったが,山口県産ニホンジカのタイレリアp33/34遺伝子の相同性は,他地域のものに比べて塩基配列で68.7〜70.1%,アミノ酸配列で64,1〜70.0%と低く,ニホンジカのタイレリアには遺伝的に異なる株が存在することが示唆された. The 18S rRNA gene and the piroplasm major immunodominant protein gene (p33/34) of Theileria from various subspecies of sika deer in 8 different locations of Japan were analyzed. The similarity between 633 bp partial sequences of the 18S rRNA gene among various subspecies of sika deer was found to be between 99.7% and 100%. While the percent identities of the 412 bp partial p33/34 gene sequence and deduced amino acid sequences between Theileria of sika deer from Yamaguchi Prefecture and those found in deer from other Prefectures, were comparatively low, 68.7% to... |
|
Palavras-chave: Cervue nipon; Divergence; Theileria sp. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4004 |
| |
|
| |
|
|
| LEE, Hsu-Hsun; KIDA, Katsuya; MIURA, Ryotaro; INOKUMA, Hisashi; MIYAMOTO, Akio; KAWASHIMA, Chiho; HANEDA, Shingo; MIYAKE, Yoh-Ichi; MATSUI, Motozumi; 猪熊, 壽; 宮本, 明夫; 川島, 千帆; 羽田, 真悟; 松井, 基純. |
| In peripartum dairy cows, insulin resistance (IR) increases to adjust the direction of energy to lactation after calving. To investigate the effect of prepartum IR on postpartum reproductive performance, the insulin tolerance test (ITT) was applied to 15 cows at 3 weeks (Pre21) and 10 days (Pre10) before the predicted calving date. Blood glucose area under the curve (AUCglu) within 120 min after administration of 0.05 IU/kg-BW insulin was calculated. The occurrence of first ovulation, days to first artificial insemination (AI) and first AI conception rate were recorded. Nutritional status postpartum was evaluated by blood chemical analysis. Based on AUCglu changes from Pre21 to Pre10, cows were classified into either the AUC-up group (AUCglu increase, n=5)... |
|
Palavras-chave: Dairy cows; Glucose metabolism; Insulin tolerance test; Reproductive performance; Transition period. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4129 |
| |
|
|
| SASAKI, Naoki; MINAMI, Takuto; YAMADA, Kazutaka; SATOH, Motoyoshi; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; YAMADA, Haruo; 佐々木, 直樹; 山田, 一孝; 猪熊, 壽; 古林, 与志安; 古岡, 秀文. |
| A 17-year-old, gray Thoroughbred gelding was presented with recurrent epistaxis following exercise for over one year. Multidetector-row computed tomographic (MDCT) examination of the head performed under general anesthesia indicated the presence of malignant melanoma. MDCT examination revealed the exact location and size of masses in the right guttural pouch first identified by endoscopy and in the left guttural pouch unidentified by endoscopy. MDCT examination revealed the presence of a blood clot that filled the right maxillary sinus. The horse was euthanized due to poor prognosis as a riding horse and the owner's decision, and MDCT findings were confirmed by postmortem examination. MDCT is suggested as a useful technique for diagnosis of head problems... |
|
Palavras-chave: Equine; Malignant melanoma; MDCT. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3104 |
| |
|
|
| 岸本, 海織; 住谷, 峻; 李, 奇子; 清水, 純一郎; 山川, 和宏; 滄木, 孝弘; 羽田, 真悟; 松本, 高太郎; 石井, 三都夫; 佐々木, 直樹; 猪熊, 壽; 山田, 一孝; KISHIMOTO, Miori; SUMIYA, Takashi; LEE, Ki-Ja; SHIMIZU, Junichiro; YAMAKAWA, Kazuhiro; AOKI, Takahiro; HANEDA, Shingo; MATSUMOTO, Kotaro; ISHII, Mitsuo; SASAKI, Naoki; INOKUMA, Hisashi; YAMADA, Kazutaka. |
| 牛臨床におけるComputed Radiography (CR) の有用性を確立するため，管球-カセッテ間距離およびImaging Plate (IP) の読み取り時間が画質に与える影響について検討した.管球-カセッテ間距離は画質の視覚的スコア(粒状性，鮮鋭性)に影響しなかった. IPの読み取り時間は画質の鮮鋭性と相関しなかったが，粒状性と相関が認められた.しかし読み取り時聞が12時間以内の場合には問題にならない程度であると考えられた.管球-力セッテ間距離を再現よく保つことが難しく， IP読み取り時間のばらつきが想定される牛の臨床画像診断においてCRは有用なX線撮影システムであると考えられた. The purpose of this study was to investigate the effect of X-ray tube-cassette distance and image scanning time on image quality to establish the benefit of Computed Radiography (CR) in bovine clinical practice. The tube-cassette distance had no effect on the visual evaluation score (graininess and sharpness). The image scanning time correlated with graininess but not sharpness. From these results，it was concluded that accidental variations in the tube-cassette... |
|
Palavras-chave: 牛; Computed Radiography (CR); フェーデイング; Imaging Plate (IP); ポータブルX線発生装置; Bovine; Computed Radiography (CR); Fading; Imaging Plate (lP); Portable x-ray generator. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2939 |
| |
|
|
| 竹内, 俊彦; 吉本, 薫; 駒形, 真; 福中, 守人; 古林, 与志安; 松本, 高太郎; 猪熊, 壽; TAKEUCHI, Toshihiko; YOSHIMOTO, Kaoru; KOMAGATA, Makoto; FUKUNAKA, Morito; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi. |
| 5歳5カ月齢のホルスタイン種雌牛が，発熱，食欲不振及び胎盤停滞を呈した．悪露排出及び子宮の腫脹は治療で改善がみられなかった．体表リンパ節の腫大はみられなかったが，直腸検査で子宮の硬結を認めたため，牛白血病を疑った．病理解剖では腹腔内リンパ節の腫大，胃漿膜面の腫瘤形成及び子宮壁の肥厚が認められた．また，組織所見ではこれら組織へのリンパ球様腫瘍細胞の浸潤が認められた．腫瘍細胞はB細胞マーカー陽性であった．牛白血病ウイルス（BLV）抗体は陰性であったが，末梢血のBLVプロウイルス検査では陽性が確認され，本症例におけるBLVの関与が示唆された． A 5-year-5-month old Holstein cow was presented with fever, anorexia, and retained placenta. Postnatal vaginal discharge and enlargement of the uterus did not improve after treatment. Although the superficial lymph nodes were not enlarged, enzootic bovine leukosis was suspected because of the enlarged and indurated uterus. An autopsy revealed enlargement of most abdominal lymph nodes, tumor mass formation on the omasum and abomasum, and thickening of the uterine wall. Histologically, there... |
|
Palavras-chave: 牛白血病ウイルス; 慢性子宮内膜炎; 地方病性牛白血病; Bovine leukosis virus; Chronic endometritis; Enzootic bovine leukosis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3555 |
| |
|
|
| OKAMURA, Koichi; SASAKI, Naoki; FUKUNAKA, Morito; YAMADA, Haruo; INOKUMA, Hisashi; 佐々木, 直樹; 猪熊, 壽. |
| The prokinetic effect of the 5-HT4 receptor agonist mosapride was evaluated in seven healthy thoroughbreds. Mosapride was orally administerd at doses of 0.5, 1.0 or 1.5 mg/kg. The breath 13CO2/12CO2 rate (Δ13CO2), an indirect indicator for the rate of gastric empting, was measured at appropriate points for 4 hr after drug administration. There was a significant increase compared with the control value at 15, 20 and 165 min for 0.5 mg/kg, 30 min at 1.0 mg/kg and 165 min for 1.5 mg/kg. The results suggest that mosapride may facilitate the gastric emptying in horses. |
|
Palavras-chave: Equine; Gastric emptying rate; Mosapride citrate. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4019 |
| |
|
| |
|
|
| 猪熊, 壽; 吉林台; 下田, 崇; 富樫, 義彦; 古林, 与志安; 古岡, 秀文; 佐藤, 基佳; 石井, 三都夫; INOKUMA, Hisashi; Jilintai; SHIMODA, Takashi; TOGASHl, Yoshihiko; KOBAYASHl, Yoshiyasu; FURUOKA, Hidefumi; SATHO, Motoyoshi; ISHI, Mitsuo. |
| 1 歳10 カ月のホルスタイン種育成雌牛が，初診時食欲不振，削痩，チアノーゼ，肺音粗励，発咳等の症状を呈した． さらに第14病日には頻脈，頸静脈拍動等の循環器症状が認められた．血液および血液生化学検査により慢性炎症像が， また心エコー検査により右心房内に三尖弁と付着しない高エコー占拠性病変が観察された．本症例は病理解剖により後 大静脈血栓症と診断されたが，血栓が心臓内において描出されたまれな症例と思われた． A 22-month-old，Holstein heifer was presented with anorexia，emaciation，cyanosis，rough vesicular breathing， and coughing on the first day. On day 14， cardiovascular symptoms such as tachycardia and jugular vein pulsation were found. A blood and blood biochemical examination revealed chronic inflammation. High echogenic obstruction without any attachment to the tricuspid valve was observed in the right atrium by echocardiograph. Pathological findings confirmed a diagnosis of caudal vena cava thrombosis. It was believed to be a rare case with thrombus in the... |
|
Palavras-chave: 後大静脈血栓症; 心エコー検査; Caudal vena cava thrombosis; Echocardiography. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2805 |
| |
|
| |
|
| |
|
|
| TAGAWA, Michihito; YBANEZ, Adrian P.; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions... |
|
Palavras-chave: Bovine hemoplasma; Direct PCR; Japan; Risk factor. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3838 |
| |
|
|
| TAWFEEQ, Mohammad Monir; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽. |
| Expression of six selective genes in peripheral blood cells was evaluated as diagnostic biomarkers for enzootic bovine leukosis (EBL) by using 10 EBL and 15 clinically healthy cattle. The clinically healthy cattle generally showed lower gene expression levels. Although wide variations of gene expression were found in some clinical cases of EBL, 4 and 5 among 10 EBL cattle showed higher expression of interleukin 2 receptor gene (IL2R) and Wilms’ tumor gene (WT1), respectively. Expression of IL2R in peripheral blood cells in EBL cattle was statistically increased; however, the lower sensitivity and higher variation in the gene expressions among clinical cases of EBL would be problems as diagnostic biomarkers. |
|
Palavras-chave: Biomarkers; Enzootic bovine leukosis; Gene expression; QRT-PCR. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3868 |
| |
|
|
| 松本, 高太郎; 村上, 智亮; 菅生, 樹春; 山田, 一孝; 古林, 与志安; 松井, 高峯; 猪熊, 壽; MATSUMOTO, Kotaro; MURAKAMI, Tomotaka; SUGO, Kiharu; YAMADA, Kazutaka; KOBAYASHI, Yoshiyasu; MATSUI, Takane; INOKUMA, Hisashi. |
| 17日齢のホルスタイン種雌子牛が，神経症状を示した. 病畜は頭部を左後方に折り曲げて伏臥姿勢を示し，時折自力で起立したがすぐに倒れ歩行不能であった. 神経学的検査では右の対光反射，威嚇瞬き反射，眼瞼反射， および音響耳介反射が消失していた. ビタミンB1，およびデキサメサゾンの投与により第7病日に歩行可能となったものの左への斜頸・旋回が認められ，神経学的検査所見に改善は認められなかった. 第7病日に病理解剖を行い，肉眼的には橋の断面右側に直径1cm大の膿瘍を認め，嫌気培養にてFusobacterium necrophorumが検出された. 左側への斜頸，聴覚の消失等の脳神経疾状は，橋の右側の膿瘍により内耳神経等の脳神経核が圧迫された結果出現した聴覚障害，平衡障害のためと考えられた. A 17-day-0ld female Holstein calf exhibited the following neurological symptoms: the calf bent its neck to the left，turning its face backward and could stand alone，but would fall within a short time. Neurological examination further revealed losses of pupillary light reflex， menace response，palpebral reflex，and hearing on the right side. After administration of dexamethasone and vitamin B1，the calf was able to walk... |
|
Palavras-chave: 脳幹部膿瘍; 牛; Fusobacterium necrophorum; Brain stem abscess; Cattle. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2945 |
| |
|
| |
|
|
| TAWFEEQ, Mohammad Monir; TAGAWA, Michihito; ITOH, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽. |
| A 49-month-old Holstein cow with anorexia, tachypnea, enlarged peripheral lymph nodes, and difficulty standing up was suspected of bovine leukosis. Hematological examination revealed lymphocytosis with the presence of neoplastic cells. Increased total lactate dehydrogenase (LDH) activity, isozymes of LDH-2 and LDH-3 activities and thymidine kinase activity were observed. Cytological findings of fine needle aspiration of subiliac lymph nodes indicated lymphosarcoma. Histopathology and antibody analysis confirmed the diagnosis of enzootic bovine leukosis, a B-cell bovine lymphoma caused by bovine leukemia virus. Gene expressions known as biomarkers of hematopoietic neoplasia in human were also examined in the present case. Increased messenger RNA expression... |
|
Palavras-chave: Biomarker; Bovine leukosis; Gene expression. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4096 |
| |
| Registros recuperados: 77 | |
|
|
|
