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Registros recuperados: 146 | |
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Mariani, Charlotte; Desdouits, Marion; Favard, Cyril; Benaroch, Philippe; Muriaux, Delphine M.. |
HIV-1 is an RNA enveloped virus that preferentially infects CD4(+) T lymphocytes and also macrophages. In CD4(+) T cells, HIV-1 mainly buds from the host cell plasma membrane. The viral Gag polyprotein targets the plasma membrane and is the orchestrator of the HIV assembly as its expression is sufficient to promote the formation of virus-like particles carrying a lipidic envelope derived from the host cell membrane. Certain lipids are enriched in the viral membrane and are thought to play a key role in the assembly process and the envelop composition. A large body of work performed on infected CD4(+) T cells has provided important knowledge about the assembly process and the membrane virus lipid composition. While HIV assembly and budding in macrophages is... |
Tipo: Text |
Palavras-chave: HIV-1; Lipids; Assembly; Gag; CD4(+) T cells; Macrophages. |
Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00344/45490/45040.pdf |
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Jauffrais, Thierry; Lekieffre, Charlotte; Schweizer, Magali; Jesus, Bruno; Metzger, Edouard; Geslin, Emmanuelle. |
The aim of this work is to document the complex nutritional strategy developed by kleptoplastic intertidal foraminifera. We study the mixotrophic ability of a common intertidal foraminifer, E. williamsoni, by (1) investigating the phylogenetic identity of the foraminiferal kleptoplasts, (2) following their oxygenic photosynthetic capacity, and (3) observing the modification in cellular ultrastructural features in response to photoautotrophic conditions. This was achieved by coupling molecular phylogenetic analyses and TEM observations with non-destructive measurements of kleptoplast O2 production over a 15-day experimental study. Results show that the studied E. williamsoni actively selected kleptoplasts mainly from pennate diatoms and had the ability to... |
Tipo: Text |
Palavras-chave: Foraminifera; Electron microscopy; DNA barcoding; Kleptoplasty; Lipids. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00487/59894/63074.pdf |
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Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Berge, Jean-pascal; Del Pino, Victoria; Uronen, Pauliina; Legrand, Catherine. |
Instead of sole nutrient starvation to boost algal lipid production, we addressed nutrient limitation at two different seasons (autumn and spring) during outdoor cultivation in flat panel photobioreactors. Lipid accumulation, biomass and lipid productivity and changes in fatty acid composition of Nannochloropsis oculata were investigated under nitrogen (N) limitation (nitrate:phosphate N:P 5, N:P 2.5 molar ratio). N. oculata was able to maintain a high biomass productivity under N-limitation compared to N-sufficiency (N:P 20) at both seasons, which in spring resulted in nearly double lipid productivity under N-limited conditions (0.21 g L−1 day−1) compared to N-sufficiency (0.11 g L−1 day−1). Saturated and monounsaturated fatty acids increased from 76% to... |
Tipo: Text |
Palavras-chave: Microalgae; Outdoor; Nannochloropsis; Large-scale; Lipids; Fatty acids; Nitrogen manipulation; Seasonal changes; Biofuels; High value products. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29564/27902.pdf |
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Emerenciano, Mauricio; Cuzon, Gerard; Mascaro, Maite; Arevalo, Miguel; Norena-barroso, Elsa; Jeronimo, Gilberto; Racotta, Ilie; Gaxiola, Gabriela. |
A 30-day trial was performed to evaluate the reproductive performance of wild and 2nd generation domesticated Farfantepenaeus duorarum broodstock. Changes in biochemical composition and fatty acids (FA) profile in the 1st and 4th spawn order females were used as indicators of nutritional condition. Wild population of F. duorarum presented significantly better reproductive outcomes as compared to domesticated ones. Wild spawners achieved significantly higher number of eggs per spawn, number of egg per spawn per g of spawner's body weight and number of nauplii per spawn (P<0.05). Latency period was significantly shorter (P<0.05) for wild than for domesticated females (11 vs 17 days). Wild spawners also presented less mortality (6 vs 12%), higher number... |
Tipo: Text |
Palavras-chave: Fatty acids; F. duorarum; Lipids; Pink shrimp; Nutrition; Reproduction. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00079/19042/17214.pdf |
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Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe. |
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5... |
Tipo: Text |
Palavras-chave: Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00268/37962/36086.pdf |
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Rico Villa, Benjamin; Le Coz, Jean-rene; Mingant, Christian; Robert, Rene. |
Microalgae commonly used as feed for bivalves, Pavlova lutheri (P), Isochrisis affinis galbana (T) and Chaetoceros calcitrans forma pumilum (Cp), were fed to Pacific oyster Crassostrea gigas to assess their nutritional value for larval development and metamorphosis during two experiments. Monospecific, bispecific and trispecific diets were firstly evaluated during 3 weeks from D larvae to young postlarvae. Then bispecific diets, based on different T and Cp proportions, were assessed during a similar period. Concurrently, ingestion was studied through the whole larval and postlarval development for each diet and/or diet mixture. Because lipids are assumed to be a key nutrient for bivalves, biochemical analysis was undertaken on the second set of trials... |
Tipo: Text |
Palavras-chave: Lipids; Grazing; Metamorphosis; Growth; Larvae; Crassostrea gigas. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1707.pdf |
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Brosset, Pablo; Fromentin, Jean-marc; Menard, Frederic; Pernet, Fabrice; Bourdeix, Jean-herve; Bigot, Jean-louis; Van Beveren, Elisabeth; Perez Rodia, Maria A.; Choy, Sandrine; Saraux, Claire. |
As condition is a key variable in population dynamics (especially for survival, growth and reproduction), the use of well-defined and accurate fish condition indices is capital. In particular, condition indices (morphometric, bioenergetic and biochemical) have never been compared and validated for the European anchovy Engraulis encrasicolus, the European pilchard Sardina pilchardus and the European sprat Sprattus sprattus. The accuracy of two indirect methods, the morphometric relative condition index Kn and the bioenergetics index determined with the Distell Fish Fatmeter was investigated by comparing with a direct measure of relative lipid content carried out with a thin layer chromatography-flame ionization detector. Estimations from the fatmeter... |
Tipo: Text |
Palavras-chave: Anchovy; Fatmeter; Lipids; Mediterranean Sea; Sardine; Sprat. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00226/33741/32304.pdf |
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Garnier, Matthieu; Bougaran, Gael; Pavlovic, Marija; Berard, Jean-baptiste; Carrier, Gregory; Charrier, Aurelie; Le Grand, Fabienne; Lukomska, Ewa; Rouxel, Catherine; Schreiber, Nathalie; Cadoret, Jean-paul; Rogniaux, Helene; Saint-jean, Bruno. |
Haptophytes are a diverse monophyletic group with a worldwide distribution, known to be significantly involved in global climate regulation in their role as a carbon sink. Because nitrogen is a major limiting macronutrient for phytoplankton in oceans and for cultures of microalgae, understanding the involvement of nitrogen availability in haptophyte carbon partitioning is of global and biotechnological importance. Here, we made an ecophysiological study coupled with comprehensive large scale proteomic analysis to examine differences of behavior in reaction to nitrogen availability changes between a wild type strain of Tisochrysis lutea (WTc1) and a mutant strain (2Xc1) known to accumulate more storage lipids. Strains were grown in chemostats and studied... |
Tipo: Text |
Palavras-chave: Algae; Isochrysis; Lipids; Nitrogen; Reverse genomic: proteomic. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00356/46702/46904.pdf |
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Pethybridge, H.; Daley, R.; Virtue, P.; Butler, E. C. V.; Cossa, Daniel; Nichols, P. D.. |
Total mercury (Hg) concentrations and lipid composition data, including fatty acid profiles, for 61 midtrophic species (fish, cephalopods, crustaceans) collected from continental slope waters off south-east Australia were examined. Overall, Hg concentrations were greatest in fish (0.01-0.30 mu g g(-1) ww) (with highest content found in barracouta (Thyrsites atun) and whiptails (Coelorinchus fasciatus)), compared with cephalopods (0.01 and 0.17 mu g g(-1) ww) and crustaceans (<0.04 mu g g(-1) ww). Lipid composition varied between species and within habitat (mesopelagic, bathypelagic and benthic). Mean total lipid content ranged from 0.5 to 13.2% ww, and in most species was dominated by triacylglycerols and phospholipids. In fish and squid, fatty acids... |
Tipo: Text |
Palavras-chave: Deep-sea; Ecosystem dynamics; Fatty acids; Lipids; Mercury; Mid-trophic prey; Mid-water. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00016/12685/9655.pdf |
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Eymard, Sylvie. |
Critical steps of the process of horse mackerel surimi manufacturing were identified. This led to propose technical and chemical solutions in order to limit development of lipid oxidation reactions. In this way, analytical methods of lipids and lipid oxidation compounds were adapted to the studied products. A method to determine hydroperoxyde concentrations was developed. The front face fluorescence technique was tested to evaluate the degree of surimi alteration. Lipid oxidation kinetics were determined during horse mackerel storage on ice or at 17°C. The formation of lipid oxidation products was followed during manufacturing and horse mackerel surimi frozen storage. The impact of raw material freshness on the development of lipid oxidation during surimi... |
Tipo: Text |
Palavras-chave: Processing; Conservation; Surimi; Pelagic fish; Lipid oxidation; Lipids; Transformation; Conservation; Surimi; Petits pélagiques; Oxydation des lipides; Lipides. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/these-2.pdf |
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Ben Kheder, Rym; Moal, Jeanne; Robert, Rene. |
The effect of four temperatures, ranging between 17 degrees C and 32 degrees C, was studied on development and lipid reserve management of Crassostrea gigas larvae. No effect of temperature was found on larval mortality, as high survival (>90%) was recorded before competence at all temperatures studied. Temperature did, nonetheless, have a strong effect on growth and settlement success. At low temperature (17 degrees C), larvae competent to metamorphose were only observed from day 23 and only a low percentage finally achieved metamorphosis (12%). The opposite was seen at temperatures >= 27 degrees C: larval competence appeared at day 18 and led to high rates of metamorphosis (60-90%). This difference at settlement seemed to be linked to larval... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Temperature; Lipids; Physiological indices. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00019/13046/10146.pdf |
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Bodoy, A; Geiaron, P; Garnier, Jacqueline; Heurtebise, S. |
Two different biomasses (100 g multiplied by m super(-2) and 1 kg multiplied by m super(-2)) of oysters were put in identical sea-water ponds, thus leading to different trophic conditions, corresponding to rearing techniques. Changes in growth, biochemical composition and sexual maturation were recorded for two age groups. The first one included 9 month-old oysters and the second one 21 month-old oysters, at the beginning of the experiment. For both age groups, significant differences were observed in growth and biochemical composition. Lipids were accumulated faster for the lower biomass. However, the only difference concerning glycogen was observed on the older oysters. They showed a peak at the lowest biomass and a decrease at the larger biomass. Sexual... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Bivalvia; Sea water; Oyster culture; Glycogen; Age; Chemical composition; Lipids; Biochemistry; Sexual maturity; Rearing; Pond culture; Biomass; Growth; Density. |
Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-916.pdf |
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Registros recuperados: 146 | |
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