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Registros recuperados: 146
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Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages ArchiMer
Mariani, Charlotte; Desdouits, Marion; Favard, Cyril; Benaroch, Philippe; Muriaux, Delphine M..
HIV-1 is an RNA enveloped virus that preferentially infects CD4(+) T lymphocytes and also macrophages. In CD4(+) T cells, HIV-1 mainly buds from the host cell plasma membrane. The viral Gag polyprotein targets the plasma membrane and is the orchestrator of the HIV assembly as its expression is sufficient to promote the formation of virus-like particles carrying a lipidic envelope derived from the host cell membrane. Certain lipids are enriched in the viral membrane and are thought to play a key role in the assembly process and the envelop composition. A large body of work performed on infected CD4(+) T cells has provided important knowledge about the assembly process and the membrane virus lipid composition. While HIV assembly and budding in macrophages is...
Tipo: Text Palavras-chave: HIV-1; Lipids; Assembly; Gag; CD4(+) T cells; Macrophages.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00344/45490/45040.pdf
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Temporal variability in the biochemical composition of sedimentary organic matter in an intertidal flat of the Galician coast (NW Spain) ArchiMer
Cividanes, S; Incera, M; Lopez, J.
Temporal variations and spatial distribution of sedimentary organic matter composition were investigated over a one year period in an intertidal flat of the NW Spain. Sediment samples were collected from 0 to 25 cm depth, every three months, from January 1997 to January 1998 at three tidal levels (high, medium and low). Changes in the elemental and biochemical composition were assessed to gather information on temporal and spatial fluctuations in quantity and quality of sedimentary organic matter potentially available to benthic deposit-feeder nutrition. Organic matter content was significantly higher at the medium tidal level, while minimum values were found at the high tidal level. The different biochemical classes of organic compounds exhibited...
Tipo: Text Palavras-chave: Matière organique sédimentaire; Lipide; Protéine; Hydrate de carbone; Sédiment intertidal; Sedimentary organic matter; Lipids; Proteins; Carbohydrates; Intertidal sediments.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00322/43324/42930.pdf
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Fatty acid composition in deep hydrothermal vent symbiotic bivalves. ArchiMer
Ben-mlih, F; Marty, Jc; Fiala-médioni, A.
Fatty acids in deep hydrothermal vent bivalves have been analyzed. Their composition is completely different from that of a littoral mussel collected in the Mediterranean sea. The distribution of fatty acids in the littoral mussel is characterized by a predominance of polyunsaturated fatty acids (20:5n-3, 22:6n-3) reflecting the planktonic origin of the food. Vent bivalve fatty acid distribution is dominated by an abundance of the monounsaturated acids (double bond in the n-7 position) 16:1n-7, 18:1n-7, and 20:1n-7 which are clearly of bacterial origin and give an indication of the symbiotic bacterial activity in the bivalves. Differences between the fatty acid composition of the bivalves from two hydrothermal sites (13 degrees N and Galapagos) and...
Tipo: Text Palavras-chave: Bacterial endosymbionts associated with bivalve gills; Relationships between bacteria and hosts; Ecological conditions; Species biochemistry; Lipids; Fatty acids; Biomarkers.
Ano: 1992 URL: https://archimer.ifremer.fr/doc/00667/77873/80020.pdf
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Response of a kleptoplastidic foraminifer to heterotrophic starvation: photosynthesis and lipid droplet biogenesis ArchiMer
Jauffrais, Thierry; Lekieffre, Charlotte; Schweizer, Magali; Jesus, Bruno; Metzger, Edouard; Geslin, Emmanuelle.
The aim of this work is to document the complex nutritional strategy developed by kleptoplastic intertidal foraminifera. We study the mixotrophic ability of a common intertidal foraminifer, E. williamsoni, by (1) investigating the phylogenetic identity of the foraminiferal kleptoplasts, (2) following their oxygenic photosynthetic capacity, and (3) observing the modification in cellular ultrastructural features in response to photoautotrophic conditions. This was achieved by coupling molecular phylogenetic analyses and TEM observations with non-destructive measurements of kleptoplast O2 production over a 15-day experimental study. Results show that the studied E. williamsoni actively selected kleptoplasts mainly from pennate diatoms and had the ability to...
Tipo: Text Palavras-chave: Foraminifera; Electron microscopy; DNA barcoding; Kleptoplasty; Lipids.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59894/63074.pdf
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Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata ArchiMer
Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Berge, Jean-pascal; Del Pino, Victoria; Uronen, Pauliina; Legrand, Catherine.
Instead of sole nutrient starvation to boost algal lipid production, we addressed nutrient limitation at two different seasons (autumn and spring) during outdoor cultivation in flat panel photobioreactors. Lipid accumulation, biomass and lipid productivity and changes in fatty acid composition of Nannochloropsis oculata were investigated under nitrogen (N) limitation (nitrate:phosphate N:P 5, N:P 2.5 molar ratio). N. oculata was able to maintain a high biomass productivity under N-limitation compared to N-sufficiency (N:P 20) at both seasons, which in spring resulted in nearly double lipid productivity under N-limited conditions (0.21 g L−1 day−1) compared to N-sufficiency (0.11 g L−1 day−1). Saturated and monounsaturated fatty acids increased from 76% to...
Tipo: Text Palavras-chave: Microalgae; Outdoor; Nannochloropsis; Large-scale; Lipids; Fatty acids; Nitrogen manipulation; Seasonal changes; Biofuels; High value products.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00184/29564/27902.pdf
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Reproductive performance, biochemical composition and fatty acid profile of wild-caught and 2nd generation domesticated Farfantepenaeus duorarum (Burkenroad, 1939) broodstock ArchiMer
Emerenciano, Mauricio; Cuzon, Gerard; Mascaro, Maite; Arevalo, Miguel; Norena-barroso, Elsa; Jeronimo, Gilberto; Racotta, Ilie; Gaxiola, Gabriela.
A 30-day trial was performed to evaluate the reproductive performance of wild and 2nd generation domesticated Farfantepenaeus duorarum broodstock. Changes in biochemical composition and fatty acids (FA) profile in the 1st and 4th spawn order females were used as indicators of nutritional condition. Wild population of F. duorarum presented significantly better reproductive outcomes as compared to domesticated ones. Wild spawners achieved significantly higher number of eggs per spawn, number of egg per spawn per g of spawner's body weight and number of nauplii per spawn (P<0.05). Latency period was significantly shorter (P<0.05) for wild than for domesticated females (11 vs 17 days). Wild spawners also presented less mortality (6 vs 12%), higher number...
Tipo: Text Palavras-chave: Fatty acids; F. duorarum; Lipids; Pink shrimp; Nutrition; Reproduction.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00079/19042/17214.pdf
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Relations entre le contenu lipidique des ovocytes de Pecten maximus (Mollusque, bivalve) et les performances des larves en élevage ArchiMer
Le Pennec, Marcel; Gueguen,.
The lipid content of spawned oocytes of Pecten maximus was studied by image analysis of semi -thin histological sections and a lipid index was defined. The female gametes were obtained by thermic stimulations of mature adults scallops from different populations after out of season conditioning or sampled in situ during the spawning season. After fertilization, the larval development was followed. The results reveal a large variability in lipid content of normal oocytes. The lipid content can be regarded as homogeneous inside a spawning but the heterogeneity is greater for wild scallops than for conditioned ones. A strong relationship appears between the lipid index and the hatching success, and the number of abnormal larvae after two days rearing. But no...
Tipo: Text Palavras-chave: Mollusques; Bivalves; Pecten maximus; Ovocytes; Lipides; Elevages larvaires; Mollusc; Bivalve; Pecten maximus; Oocytes; Lipids; Larval development.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00022/13345/10379.pdf
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Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas ArchiMer
Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe.
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5...
Tipo: Text Palavras-chave: Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37962/36086.pdf
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Influence of phytoplankton diet mixtures on microalgae consumption, larval development and settlement of the Pacific oyster Crassostrea gigas (Thunberg) ArchiMer
Rico Villa, Benjamin; Le Coz, Jean-rene; Mingant, Christian; Robert, Rene.
Microalgae commonly used as feed for bivalves, Pavlova lutheri (P), Isochrisis affinis galbana (T) and Chaetoceros calcitrans forma pumilum (Cp), were fed to Pacific oyster Crassostrea gigas to assess their nutritional value for larval development and metamorphosis during two experiments. Monospecific, bispecific and trispecific diets were firstly evaluated during 3 weeks from D larvae to young postlarvae. Then bispecific diets, based on different T and Cp proportions, were assessed during a similar period. Concurrently, ingestion was studied through the whole larval and postlarval development for each diet and/or diet mixture. Because lipids are assumed to be a key nutrient for bivalves, biochemical analysis was undertaken on the second set of trials...
Tipo: Text Palavras-chave: Lipids; Grazing; Metamorphosis; Growth; Larvae; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1707.pdf
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Measurement and analysis of small pelagic fish condition: A suitable method for rapid evaluation in the field ArchiMer
Brosset, Pablo; Fromentin, Jean-marc; Menard, Frederic; Pernet, Fabrice; Bourdeix, Jean-herve; Bigot, Jean-louis; Van Beveren, Elisabeth; Perez Rodia, Maria A.; Choy, Sandrine; Saraux, Claire.
As condition is a key variable in population dynamics (especially for survival, growth and reproduction), the use of well-defined and accurate fish condition indices is capital. In particular, condition indices (morphometric, bioenergetic and biochemical) have never been compared and validated for the European anchovy Engraulis encrasicolus, the European pilchard Sardina pilchardus and the European sprat Sprattus sprattus. The accuracy of two indirect methods, the morphometric relative condition index Kn and the bioenergetics index determined with the Distell Fish Fatmeter was investigated by comparing with a direct measure of relative lipid content carried out with a thin layer chromatography-flame ionization detector. Estimations from the fatmeter...
Tipo: Text Palavras-chave: Anchovy; Fatmeter; Lipids; Mediterranean Sea; Sardine; Sprat.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00226/33741/32304.pdf
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Use of a lipid rich strain reveals mechanisms of nitrogen limitation and carbon partitioning in the haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu; Bougaran, Gael; Pavlovic, Marija; Berard, Jean-baptiste; Carrier, Gregory; Charrier, Aurelie; Le Grand, Fabienne; Lukomska, Ewa; Rouxel, Catherine; Schreiber, Nathalie; Cadoret, Jean-paul; Rogniaux, Helene; Saint-jean, Bruno.
Haptophytes are a diverse monophyletic group with a worldwide distribution, known to be significantly involved in global climate regulation in their role as a carbon sink. Because nitrogen is a major limiting macronutrient for phytoplankton in oceans and for cultures of microalgae, understanding the involvement of nitrogen availability in haptophyte carbon partitioning is of global and biotechnological importance. Here, we made an ecophysiological study coupled with comprehensive large scale proteomic analysis to examine differences of behavior in reaction to nitrogen availability changes between a wild type strain of Tisochrysis lutea (WTc1) and a mutant strain (2Xc1) known to accumulate more storage lipids. Strains were grown in chemostats and studied...
Tipo: Text Palavras-chave: Algae; Isochrysis; Lipids; Nitrogen; Reverse genomic: proteomic.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00356/46702/46904.pdf
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Lipid and mercury profiles of 61 mid-trophic species collected off south-eastern Australia ArchiMer
Pethybridge, H.; Daley, R.; Virtue, P.; Butler, E. C. V.; Cossa, Daniel; Nichols, P. D..
Total mercury (Hg) concentrations and lipid composition data, including fatty acid profiles, for 61 midtrophic species (fish, cephalopods, crustaceans) collected from continental slope waters off south-east Australia were examined. Overall, Hg concentrations were greatest in fish (0.01-0.30 mu g g(-1) ww) (with highest content found in barracouta (Thyrsites atun) and whiptails (Coelorinchus fasciatus)), compared with cephalopods (0.01 and 0.17 mu g g(-1) ww) and crustaceans (<0.04 mu g g(-1) ww). Lipid composition varied between species and within habitat (mesopelagic, bathypelagic and benthic). Mean total lipid content ranged from 0.5 to 13.2% ww, and in most species was dominated by triacylglycerols and phospholipids. In fish and squid, fatty acids...
Tipo: Text Palavras-chave: Deep-sea; Ecosystem dynamics; Fatty acids; Lipids; Mercury; Mid-trophic prey; Mid-water.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00016/12685/9655.pdf
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Nutrition of the seabass Lates calcarifer ArchiMer
Cuzon, Gerard; Chou, R; Fuchs, Jacques.
The Primary Production Department of Singapore (PPD) studies covered the weaning of seabass from fingerlings stage onwards and the dietary protein requirement of early grow-out fish. Dietary protein requirement of early grow-out seabass was demonstrated to be between 40-50%, at dietary lipid level of 12%, using fishmeal protein. Nevertheless fish growth was significantly higher with trashfish feed, but apparent protein retention was significantly better with formulated feed.
Tipo: Text Palavras-chave: Lates calcarifer; Fry; Nutritional requirements; Biochemistry; Food absorption; Growth rate; Fish meal; Seed (aquaculture); Dietary deficiencies; Fingerlings; Lipids; Proteins; Diets; Pellet feeds.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1425.pdf
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Mise en évidence et suivi de l'oxydation des lipides au cours de la conservation et de la transformation du chinchard (Trachurus trachurus) : choix des procédés ArchiMer
Eymard, Sylvie.
Critical steps of the process of horse mackerel surimi manufacturing were identified. This led to propose technical and chemical solutions in order to limit development of lipid oxidation reactions. In this way, analytical methods of lipids and lipid oxidation compounds were adapted to the studied products. A method to determine hydroperoxyde concentrations was developed. The front face fluorescence technique was tested to evaluate the degree of surimi alteration. Lipid oxidation kinetics were determined during horse mackerel storage on ice or at 17°C. The formation of lipid oxidation products was followed during manufacturing and horse mackerel surimi frozen storage. The impact of raw material freshness on the development of lipid oxidation during surimi...
Tipo: Text Palavras-chave: Processing; Conservation; Surimi; Pelagic fish; Lipid oxidation; Lipids; Transformation; Conservation; Surimi; Petits pélagiques; Oxydation des lipides; Lipides.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/these-2.pdf
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Etude de la composition corporelle de la sole (Solea solea ) au cours du jeune: Influence de la temperature ArchiMer
Richard, P; Djabali, M; Lagardere, Francoise; Lagardere, J.
The effects of starvation on Solea solea at 10 and 20 degree C in sea water, were studied. At 20 degree C fishes start to die on week 4; at 10 degree C they support a longer starvation period (9 weeks). Proximate analysis shows that protein are more steadily used at 20 degree C than at 10 degree C, contrarily to the corporal lipids which are used at the same rate. Starvation results in a reduction of liver lipids and glycogen. This reduction is more pronounced at 20 degree C : 70% of hepatic glycogen is used at this temperature within the first week of starvation against 30% at 10 degree C.
Tipo: Text Palavras-chave: Solea solea; Soleidae; Pisces; Survival; Tissues; Biochemical composition; Temperature effects; Proteins; Lipids; Starvation; Metabolism.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1246.pdf
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Etude cytologique ultrastructurale des anomalies hepatiques du loup, de la daurade, et de l'anguille, induites par une alimentation artificielle ArchiMer
Bac, N; Biagianti, S; Brusle, J.
In the three species Dicentrarchus labrax, Sparus auratus , and Anguilla anguilla an artificial feeding (dry pellets) disturbs liver cells as revealed at ultrastructural level. In hepatocytes, anomalies consist of lipids storage (liposomes) considered as deviation in fatty acids metabolism. Moreover are observed cellular alterations (mitochondrial vacuolization, nuclear pycnosis) related to degenerative process. It is of great interest to understand wether liver modifications are just a reaction to an unbalanced feeding or a true trophic pathology.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Natural populations; Fish culture; Lipids; Fatty acids; Cytology; Ultrastructure; Cells; Diseases; Pellet feeds; Metabolism; Liver; Artificial feeding; Nutrition disorders.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1250.pdf
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Ontogeny of the gastrointestinal tract of marine fish larvae ArchiMer
Zambonino, Jose-luis; Cahu, Chantal.
Marine fish larvae undergo major morphological and cellular changes during the first month of life. The ontogeny of the gastrointestinal tract combines these two aspects of the larval development and is very interesting in that the timing of functional changes appears genetically hard-wired. The goal of this paper is to give an overview of the gastrointestinal development process in marine fish larvae, with particular attention to three species: sea bass; red drum; and sole, since the description of gut maturation in fish larvae was initiated during the last decade with these species. During the early stages, marine fish larvae exhibit particular digestive features. Concerning the exocrine pancreas, amylase expression decreases with age from the third week...
Tipo: Text Palavras-chave: Proteins; Pancreatic enzymes; Lipids; Larvae feeds; Intestinal enzymes; Glucides; Fish larvae; Enzyme expression; Digestion; Development; Aquaculture.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-650.pdf
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Impact of temperature on larval development and evolution of physiological indices in Crassostrea gigas ArchiMer
Ben Kheder, Rym; Moal, Jeanne; Robert, Rene.
The effect of four temperatures, ranging between 17 degrees C and 32 degrees C, was studied on development and lipid reserve management of Crassostrea gigas larvae. No effect of temperature was found on larval mortality, as high survival (>90%) was recorded before competence at all temperatures studied. Temperature did, nonetheless, have a strong effect on growth and settlement success. At low temperature (17 degrees C), larvae competent to metamorphose were only observed from day 23 and only a low percentage finally achieved metamorphosis (12%). The opposite was seen at temperatures >= 27 degrees C: larval competence appeared at day 18 and led to high rates of metamorphosis (60-90%). This difference at settlement seemed to be linked to larval...
Tipo: Text Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Temperature; Lipids; Physiological indices.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00019/13046/10146.pdf
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Croissance et qualité de l'huître ceuse Crassostrea gigas dans le bassin de Thau (Hérault) : Résultats d'une étude réalisée en 1990-1991 ArchiMer
Pichot, Paul; Landrein, Sonia; Juge, Claude.
During years 1990-1991, a study was carried ouI in order to precise the relationship between sorne physiological features and the biochemical characteristics of dry flesh and hemolymf of oyster Crassostrea gigas, cultivated in the mediterranean lagoon of Thau. Growth rates and biochemical analysis for proteins, lipids and carbohydrates were estimated. The results of this study are given. They include many other data from a study on trophic relationships developed between 1985-1989.
Tipo: Text Palavras-chave: Méditerranée; Etang de Thau; Crassostrea gigas; Croissance; Composition biochimique; Protides; Lipides; Glucides; Mediterranean; Lagoon of Thau; Crassostrea gigas; Growth; Biochemical composition; Proteins; Lipids; Carbohydrates.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00419/53031/53966.pdf
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Influence de la densite et de l'age des huitres japonaises (Crassostrea gigas ) sur la croissance et la composition biochimique en bassins de terre ArchiMer
Bodoy, A; Geiaron, P; Garnier, Jacqueline; Heurtebise, S.
Two different biomasses (100 g multiplied by m super(-2) and 1 kg multiplied by m super(-2)) of oysters were put in identical sea-water ponds, thus leading to different trophic conditions, corresponding to rearing techniques. Changes in growth, biochemical composition and sexual maturation were recorded for two age groups. The first one included 9 month-old oysters and the second one 21 month-old oysters, at the beginning of the experiment. For both age groups, significant differences were observed in growth and biochemical composition. Lipids were accumulated faster for the lower biomass. However, the only difference concerning glycogen was observed on the older oysters. They showed a peak at the lowest biomass and a decrease at the larger biomass. Sexual...
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Sea water; Oyster culture; Glycogen; Age; Chemical composition; Lipids; Biochemistry; Sexual maturity; Rearing; Pond culture; Biomass; Growth; Density.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-916.pdf
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