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Effects of blue light on the biochemical composition and photosynthetic activity of Isochrysis sp. (T-iso) ArchiMer
Marchetti, Julie; Bougaran, Gael; Jauffrais, Thierry; Lefebvre, Sebastien; Rouxel, Catherine; Saint-jean, Bruno; Lukomska, Ewa; Robert, Rene; Cadoret, Jean-paul.
In aquaculture, particularly in bivalve hatcheries, the biochemical composition of algal diets has a strong influence on larval and post-larval development. Biochemical composition is known to be related to culture conditions, among which light represents a major source of variation. The effects of blue light on biochemical composition and photosynthetic rate of Isochrysis sp. (T-iso) CCAP 927/14 were assessed in chemostat at a single irradiance (300 μmol photons m−2 s−1) and compared with white light. Two different dilution (renewal) rates were also tested: 0.7 and 0.2 d−1. Relative carbohydrate content was lower under blue light than under white light at both dilution rates, whereas chlorophyll a and photosynthesis activity were higher. In contrast,...
Tipo: Text Palavras-chave: Isochrysis; Blue light; Photosynthesis; Proximate composition; Chemostat.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00085/19599/17237.pdf
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Evolutionary Dynamics of Chloroplast Genomes in Low Light: A Case Study of the Endolithic Green Alga Ostreobium quekettii ArchiMer
Marcelino, Vanessa R.; Cremen, Ma Chiela M.; Jackson, Chistopher J.; Larkum, Anthony A. W.; Verbruggen, Heroen.
Some photosynthetic organisms live in extremely low light environments. Light limitation is associated with selective forces as well as reduced exposure to mutagens, and over evolutionary timescales it can leave a footprint on species' genomes. Here, we present the chloroplast genomes of four green algae (Bryopsidales, Ulvophyceae), including the endolithic (limestone-boring) alga Ostreobium quekettii, which is a low light specialist. We use phylogenetic models and comparative genomic tools to investigate whether the chloroplast genome of Ostreobium corresponds to our expectations of how low light would affect genome evolution. Ostreobium has the smallest and most gene-dense chloroplast genome among Ulvophyceae reported to date, matching our expectation...
Tipo: Text Palavras-chave: Genome streamlining; Photosynthesis; Rates of evolution; Boring algae; Stoichiogenomics.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00659/77094/78418.pdf
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How do microalgae perceive light in a high-rate pond? Towards more realistic Lagrangian experiments ArchiMer
Demory, David; Combe, Charlotte; Hartmann, Philipp; Talec, Amelle; Pruvost, Eric; Hamouda, Raouf; Souille, Fabien; Lamarea, Pierre-olivier; Bristeau, Marie-odile; Sainte-marie, Jacques; Rabouille, Sophie; Mairet, Francis; Sciandra, Antoine; Bernard, Olivier.
Hydrodynamics in a high-rate production reactor for microalgae cultivation affects the light history perceived by cells. The interplay between cell movement and medium turbidity leads to a complex light pattern, whose forcing effects on photosynthesis and photoacclimation dynamics are nontrivial. Hydrodynamics of high density algal ponds mixed by a paddle wheel has been studied recently, although the focus has never been on describing its impact on photosynthetic growth efficiency. In this multidisciplinary downscaling study, we first reconstructed single cell trajectories in an open raceway using an original hydrodynamical model offering a powerful discretization of the Navier-Stokes equations tailored to systems with free surfaces. The trajectory of a...
Tipo: Text Palavras-chave: Photosynthesis; Hydrodynamics; Modelling; Photoacclimation; Dunaliella salina; Non-photochemical quenching.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00445/55692/57352.pdf
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Seasonal variations in photosynthetic irradiance response curves of macrophytes from a Mediterranean coastal lagoon ArchiMer
Plus, Martin; Auby, Isabelle; Verlaque, Marc; Levavasseur, Guy.
The main photosynthesis and respiration parameters (dark respiration rate, light saturated production rate, saturation irradiance, photosynthetic efficiency) were measured on a total of 23 macrophytes of the Than lagoon (2 Phanerogams, 5 Chlorophyceae, 10 Rhodophyceae and 6 Phaeophyceae). Those measurements were performed in vitro under controlled conditions, close to the natural ones, and at several seasons. Concomitantly, measurements of pigment concentrations, carbon, phosphorous and nitrogen contents in tissues were performed. Seasonal intra-specific variability of photosynthetic parameters was found very high, enlightening an important acclimatation capacity. The highest photosynthetic capacities were found for Chlorophyceae (e.g. Monostroma obscurum...
Tipo: Text Palavras-chave: Thau lagoon; Phaeophyceae; Rhodophyceae; Chlorophyceae; Seagrasses; Seasonal variations; Oxygen method; Macrophytes; Light response curve; Photosynthesis.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-465.pdf
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Etude du biofilm microalgal des vasières intertidales : dynamique spatio-temporelle à micro-échelle et performances photosynthétiques ArchiMer
Herlory, Olivier.
The microalgal biofilm, which is present on sediment surface, is the primary production unit on bare intertidal mudlfats. In order to improve our understanding of this primary production system, a study was undertaken at microscale to analyse the structural and functional properties of this biofilm. In the first part of this work, a detailed descriptive analysis lead to the characterisation of the spatiotemporal dynamics of the physical structure of the biofilm. It turns out that the biofilm exhibits its own dynamics at the sediment surface (as a discrete compartment in the top 200 µm), but still has a functional relationship with the total available biomass (PCB) distributed within the top 1 cm (linear relationship between the maximum size of the biofilm...
Tipo: Text Palavras-chave: PAM Fluorometry; Scanning electron microscopy; Photosynthesis; Spatio temporal dynamic; Micro scale; Microphytobenthic biofilm; Intertidal mudflats; Fluorimétrie PAM; Microscopie électronique à balayage; Photosynthèse; Dynamique spatio temporelle; Micro échelle; Biofilm microphytobenthique; Vasière intertidal.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2138.pdf
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The use of high rate algal ponds for the treatment of marine effluent from a recirculating fish rearing system ArchiMer
Pagand, Pascal; Blancheton, Jean-paul; Lemoalle, J; Casellas, C.
A high rate algal pond (HRAP) system was used to treat effluent from a recirculating sea water aquaculture system in southern France. Dicentrarchus labrax L. were farmed at a high density, with effluents containing an average of 10 mg L-1 dissolved inorganic nitrogen (DIN) and 1.3 mg L-1 reactive phosphorus (RP). On a yearly basis, the algal pond removed 59% of the dissolved nitrogen and 56% of the phosphorus input, which was converted into 3.3 kg DW m(-2) algae. Green macroalgae were dominant throughout the year and the algal biomass mirrored the seasonal changes in daily irradiance and temperature. This first year study supports the possibility of treating marine aquaculture wastes using HPAPs, although conditions will have to be found to mitigate the...
Tipo: Text Palavras-chave: Nitrogen; Biofilters; Irradiance; Temperature; Photosynthesis; Growth; Culture; Chlorophyta; Nutrient Removal.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-515.pdf
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In situ growth potential of the subtidal part of green tide forming Ulva spp. stocks ArchiMer
Merceron, Michel; Antoine, Virginie; Auby, Isabelle; Morand, P.
Ulva spp., the algae most responsible for green tides in Brittany (France), are found on the foreshore and in the most beachward wave area (MBWA) of many bays during green tide phenomena. These algae have recently been seen drifting at greater depths (reaching -20 m). In view of the significant quantities of algae found at these depths, and the less favorable conditions for algal growth than in the intertidal zone, we attempted to determine if they could grow there. For that, during their maximum growth period (from May to July), algae were picked up at three stations located on the foreshore, in the MBWA and in the subtidal (deep) zones of the Bay of Douarnenez, and their nitrogen, carbon and chlorophyll a+b contents were determined, and their...
Tipo: Text Palavras-chave: Nutrients; Irradiance; Photosynthesis; Ulva sp.; Algal blooms; Eutrophication.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3527.pdf
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Les chaines alimentaires naturelles et l'aquaculture ArchiMer
Barnabe, G.
Aquaculture sets up manipulation of natural food chains. This paper describes some utilizations of photosynthetic bacteria, phytoplankton and zooplankton taken out sewage ponds, wetlands or neritic waters.
Tipo: Text Palavras-chave: Bacteria; Photosynthesis; Aquaculture; Ecosystems; Zooplankton; Phytoplankton; Food chains.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1254.pdf
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The Role of Sustained Photoprotective Non-photochemical Quenching in Low Temperature and High Light Acclimation in the Bloom-Forming Arctic Diatom Thalassiosira gravida ArchiMer
Lacour, Thomas; Lariviere, Jade; Ferland, Joannie; Bruyant, Flavienne; Lavaud, Johann; Babin, Marcel.
Thalassiosira gravida is a major Arctic diatom responsible for the under-ice spring bloom. We investigated T. gravida physiological plasticity growing it at two temperatures (0 and 5°C) and under different light intensities typically found in its natural environment. T. gravida showed remarkable thermal- and photo-acclimatory plasticity including: low light saturation parameter for growth (KE) and photosynthesis (EK), low μmax but relatively high Chl a/C, low C/N, and decreasing light-saturated carbon fixation rate (PmC) with increasing growth irradiance. T. gravida also showed remarkable photoprotective features, namely a strong sustained non-photochemical quenching (NPQs, hour kinetics relaxation) supported by a high amount of xanthophyll cycle pigments....
Tipo: Text Palavras-chave: Arctic diatom; Photosynthesis; Sustained NPQ; Irradiance; Temperature.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00462/57387/59468.pdf
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Allelochemicals of Alexandrium minutum: Kinetics of membrane disruption and photosynthesis inhibition in a co-occurring diatom ArchiMer
Long, Marc; Peltekis, Alexandra; González-fernández, Carmen; Hégaret, Helene; Bailleul, Benjamin.
Allelopathy is an efficient strategy by which some microalgae can outcompete other species. Allelochemicals from the toxic dinoflagellate Alexandrium minutum have deleterious effects on diatoms, inhibiting metabolism and photosynthesis and therefore give a competitive advantage to the dinoflagellate. The precise mechanisms of allelochemical interactions and the molecular target of allelochemicals remain however unknown. To understand the mechanisms, the short-term effects of A. minutum allelochemicals on the physiology of the diatom Chaetoceros muelleri were investigated. The effects of a culture filtrate were measured on the diatom cytoplasmic membrane integrity (polarity and permeability) using flow-cytometry and on the photosynthetic performance using...
Tipo: Text Palavras-chave: Allelochemicals; Allelopathy; Alexandrium minutum; Chaetoceros muelleri; Photosynthesis; Membrane.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00680/79191/81688.pdf
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Role des bacteries phototrophes anoxygeniques dans une partie stratifiee d'une lagune tropicale (Lagune Ebrie, Cote d'Ivoire) ArchiMer
Caumette, P.
In the deepest parts of the estuarine region of Ebrie Lagoon (Abidjan Africa, (5 degree N, 4 degree W) stratified conditions have been observed during the rainy season (May to Nov.), owing to salinity differences between oxic and anoxic layers. At the interface, 3-4 m depth, phototrophic bacteria (Chromatium, Chlorobium and Pelodictyon ) developed, forming a brown layer due to their different pigments. Their production has been estimated at 41% of the total photosynthetic production during high stratification (Sep. 1981) and their biomass was used by the copepod population for 40 to 60% of its diet. During the dry season, the water column became homogeneous and oxygen was detected to 6m depth. The brown layer of phototrophic bacteria disappeared, as well...
Tipo: Text Palavras-chave: Chlorobium; Chromatium; Primary production; Photosynthesis; Estuaries; Seasonal variations; Plankton; Stratification; Vertical distribution; Bacteria; Coastal lagoons; Trophic relationships; Biomass.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-957.pdf
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Coping with branch excision when measuring leaf net photosynthetic rates in a lowland tropical forest ArchiMer
Verryckt, Lore T.; Van Langenhove, Leandro; Ciais, Philippe; Courtois, Elodie A.; Vicca, Sara; Peñuelas, Josep; Stahl, Clément; Coste, Sabrina; Ellsworth, David S.; Posada, Juan M.; Obersteiner, Michael; Chave, Jérôme; Janssens, Ivan A..
Measuring leaf gas exchange from canopy leaves is fundamental for our understanding of photosynthesis and for a realistic representation of carbon uptake in vegetation models. Since canopy leaves are often difficult to reach, especially in tropical forests with emergent trees up to 60 m at remote places, canopy access techniques such as canopy cranes or towers have facilitated photosynthetic measurements. These structures are expensive and therefore not very common. As an alternative, branches are often cut to enable leaf gas exchange measurements. The effect of branch excision on leaf gas exchange rates should be minimized and quantified to evaluate possible bias. We compared light‐saturated leaf net photosynthetic rates measured on excised and intact...
Tipo: Text Palavras-chave: Branch cutting; Canopy physiology; French Guiana; Gas exchange; Photosynthesis; Rainforest; Stomatal conductance.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00613/72466/71949.pdf
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Transient initial phase in continuous culture of Isochrysis galbana affinis Tahiti ArchiMer
Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Kaas, Raymond; Baron, Regis.
Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density of 5 x 10(5) cell ml(-1) and a dilution rate of about 1.0 d(-1), both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and...
Tipo: Text Palavras-chave: Isochrysis; Cell quota; Photosynthesis; Steady state; Continuous culture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-597.pdf
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Effects of copper and butyltin compounds on the growth, photosynthetic activity and toxin production of two HAB dinoflagellates : the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata ArchiMer
Couet, Douglas; Pringault, Olivier; Bancon-montigny, Chrystelle; Briant, Nicolas; Poulichet, Francoise Elbaz; Delpoux, Sophie; Yahia, Ons Kefi-daly; Hela, Bengharbia; Charaf, M'Rabet; Herve, Fabienne; Rovillon, Georges-augustin; Amzil, Zouher; Laabir, Mohamed.
Controlled laboratory experiments were conducted to test the effects of copper (Cu2+) and butyltins (BuT) on the growth, photosynthetic activity and toxin content of two HABs (Harmful Algal Blooms) dinoflagellates, the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata. Microalgae were exposed to increasing concentrations of Cu2+ (10−4 to 31 nM) or BuT (0.084 to 84 nM) for seven days. When considering the growth, EC50 values were 0.16 (±0.09) nM and 0.03 (±0.02) nM of Cu2+ for A. catenella and O. cf. ovata, respectively. Regarding BuT, EC50 was 14.2 (±6) nM for O. cf. ovata, while A. catenella growth inhibition appeared at BuT concentrations ≥27 nM. Photosynthetic activity of the studied dinoflagellates decreased with increasing Cu and...
Tipo: Text Palavras-chave: Alexandrium catenella; Ostreopsis cf. ovata; Cu; Butyltin; Photosynthesis; Toxins.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00418/52970/53896.pdf
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Response of CO2-starved diatom Phaeodactylum tricornutum to light intensity transition ArchiMer
Heydarizadeh, Parisa; Boureba, Wafaa; Zahedi, Morteza; Huang, Bing; Moreau, Brigitte; Lukomska, Ewa; Couzinet-mossion, Aurelie; Wielgosz-collin, Gaetane; Martin-jezequel, Veronique; Bougaran, Gael; Marchand, Justine; Schoefs, Benoit.
In this study, we investigated the responses of Phaeodactylum tricornutum cells acclimated to 300 mu mol m(-2) s(-1) photon flux density to an increase (1000 mmol m(-2) s(-1)) or decrease (30 mmol m(-2)s(-1)) in photon flux densities. The light shift occurred abruptly after 5 days of growth and the acclimation to new conditions was followed during the next 6 days at the physiological and molecular levels. The molecular data reflect a rearrangement of carbon metabolism towards the production of phosphoenolpyruvic acid (PEP) and/or pyruvate. These intermediates were used differently by the cell as a function of the photon flux density: under low light, photosynthesis was depressed while respiration was increased. Under high light, lipids and proteins...
Tipo: Text Palavras-chave: Phaeodactylum tricornutum; Carbon metabolism; Light regulation; Photosynthesis; Chlorophyll fluorescence; Pyruvate hub.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00395/50620/52341.pdf
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Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis ArchiMer
Lacour, Thomas; Morin, Philippe-israël; Sciandra, Théo; Donaher, Natalie; Campbell, Douglas A.; Ferland, Joannie; Babin, Marcel.
During winter in the Arctic marine ecosystem, diatoms have to survive long periods of darkness caused by low sun elevations and the presence of sea ice covered by snow. To better understand how diatoms survive in the dark, we subjected cultures of the Arctic diatom Chaetoceros neogracilis to a prolonged period of darkness (1 month) and to light resupply. Chaetoceros neogracilis was not able to grow in the dark but cell biovolume remained constant after 1 month in darkness. Rapid resumption of photosynthesis and growth recovery was also found when the cells were transferred back to light at four different light levels ranging from 5 to 154 µmol photon m−2 s−1. This demonstrates the remarkable ability of this species to re-initiate growth over a wide range...
Tipo: Text Palavras-chave: Arctic microalgae; Polar night; Diatom; Darkness; Photosynthesis; Growth rate; Temperature.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00499/61066/64597.pdf
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Combined effects of global climate change and nutrient enrichment on the physiology of three temperate maerl species ArchiMer
Qui-minet, Zujaila Nohemy; Coudret, Jérôme; Davoult, Dominique; Grall, Jacques; Mendez‐sandin, Miguel; Cariou, Thierry; Martin, Sophie.
Made up of calcareous coralline algae, maerl beds play a major role as ecosystem engineers in coastal areas throughout the world. They undergo strong anthropogenic pressures, which may threaten their survival. The aim of this study was to gain insight into the future of maerl beds in the context of global and local changes. We examined the effects of rising temperatures (+3°C) and ocean acidification (−0.3 pH units) according to temperature and pH projections (i.e., the RCP 8.5 scenario), and nutrient (N and P) availability on three temperate maerl species (Lithothamnion corallioides, Phymatolithon calcareum, and Lithophyllum incrustans) in the laboratory in winter and summer conditions. Physiological rates of primary production, respiration, and...
Tipo: Text Palavras-chave: Calcification; Maerl; Nitrate; Ocean acidification; Ocean warming; Phosphate; Photosynthesis; Respiration.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00595/70722/68955.pdf
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Effect of Bentazon on Growth and Physiological Responses of Marine Diatom: Chaetoceros gracilis ArchiMer
Hourmant, Annick; Amara, Anis; Pouline, Patrick; Durand, Gael; Arzul, Genevieve; Quiniou, Francoise.
The herbicide bentazon (CASRN 25057-89-0) is extensively used in agriculture in Brittany (France) to replace atrazine. Bentazon is not readily adsorbed by soil and therefore it enters adjacent freshwater ecosystems, making its way to estuarine and marine waters areas. Information regarding its effects on marine ecosystems is scarce. Phytotoxicity assessments were conducted in the laboratory on the common diatom Chaetoceros gracilis using both the active ingredient and its formulated product (Basamais). The 3 day EC50 Using cell counts were, respectively, 150 mg/L and 60 mu g/L for bentazon active ingredient and for bentazon-formulated while cellular volume was increased. Although bentazon is known as a photosystem II inhibitor, it produced an increase of...
Tipo: Text Palavras-chave: Photosynthesis; Marine diatom; Growth; Chlorophylls; Chaetoceros gracilis; Carotenoids; Bentazon; ATP.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6637.pdf
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Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance interactions and physiological impacts ArchiMer
Dupraz, Valentin; Ménard, Dominique; Akcha, Farida; Budzinski, Hélène; Stachowski-haberkorn, Sabine.
This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine microalgae Tisochrysis lutea and Skeletonema marinoi. It also examined the single and combined effects of three of the most toxic substances on microalgal physiology. Single substances were first tested on each microalgal species to determine their respective EC50 and concentration-response relationships. The toxicity of six and seven binary mixtures was then evaluated in microplate experiments on the growth of T. lutea and S. marinoi, respectively, using two mixture modelling approaches: isobolograms and the MIXTOX tool, based on Concentration Addition (CA) or Independent Action (IA) models. Significant cases of antagonism (for both...
Tipo: Text Palavras-chave: ROS; Photosynthesis; Lipids; Synergy; Isoproturon; Metazachlor.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00488/59928/63131.pdf
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Reduction of photosynthesis before midday depression occurred: leaf photosynthesis of Fagus crenata in a temperate forest in relation to canopy position and a number of days after rainfall OAK
Koyama, Kohei; Kikuzawa, Kihachiro.
We investigated an effect of canopy position and a number of days after rainfall on reduction of photosynthetic rate in a Fagus crenata forest in summer 2008, during days when midday depression was not apparent. We compared in situ photosynthetic rate and photosynthetic rate that was calculated by photosynthetic light response curves measured in the morning. The ratio, in situ photosynthesis divided by the curve-estimated value, declined towards the end of each day for the upper leaves, but not for the lower leaves. Total photosynthesis was reduced only for the upper leaves by 12% during 5 days after the rainfall.
Palavras-chave: Photosynthesis; Stomatal conductance; Net primary production; Temperate forest; Fagus crenata Blume.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4483
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