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| Registros recuperados: 97 | |
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| GLORIA,FERNANDA JANUZZI MENDES DA; MOURÃO FILHO,FRANCISCO DE ASSIS ALVES; MENDES,BEATRIZ MADALENA JANUZZI. |
| A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. Somatic embryo formation occurred spontaneously after two subcultures, on modified MT medium supplemented with 500 mg/L of malt extract.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cultivar improvement; Polyethylene glycol; Rootstock; Tissue culture. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400008 |
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| Araruna,Elequisandra da Costa; Ribeiro-Oliveira,João Paulo; Pereira,Vanderley José; Asmar,Simone Abreu; Melo,Berildo de. |
| Abstract: The objective of this work was to evaluate how salt concentrations in media used for in vitro cultures affect the early development of “barueiro” (Dipteryx alata) seedlings obtained from the shoot apex. Two different in vitro culture media - Murashige & Skoog (MS) and wood plant medium (WPM) - in different salt concentrations (25, 50, 75, and 100%) were used. At 120 days, the D. alata seedlings in the MS medium, in its original concentration (100%), developed better than in other concentrations or in the WPM. Therefore, the MS medium was considered the most suitable for ensuring in vitro establishment because it provided greater root length (27.65 cm) and number of leaves per plant (26 leaves) than the other medium. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado biome; Culture media; Osmoregulation; Plant growth; Salt concentrations; Tissue culture. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201295 |
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| Assareh,Mohammad Hassan; Sardabi,Hossein. |
| Christ's thorn (Ziziphus spina-christi (L.) Desf.) is a cross-pollinated plant with a wide range of genetic variability in nature and, for this reason, vegetative propagation assumes importance for improvement programs. The objective of this work was to evaluate cutting, T budding and tissue culture methods for this species. Shoots of 22-25 cm length were treated by two culture media and three shoot diameters for cutting trial. The T budding treatments consisted of three and five collection dates in spring and autumn, respectively. Tissue culture nodal segments bearing axillary buds were removed from shoots of mature trees at different seasons. Experiments to determine the best disinfectant chemical, appropriate conditions and materials to prevent phenolic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cutting; T budding; Tissue culture; Media. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000500006 |
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| Sadia¹,B; Josekutty,PC; Potlakayala,SD; Patel,P; Goldman,S; Rudrabhatla,SV. |
| A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype NC+262, followed by NC+6C21 (31.3 ± 0.8), and NC+6B50 (16 ± 1.7). MS medium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Sorghum bicolor; Tissue culture; Genetic improvement; Genotypes. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200010 |
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| ALMEIDA,WELITON ANTONIO BASTOS DE; SANTANA,GESSIONEI SILVA; RODRIGUEZ,ADRIANA PINHEIRO MARTINELI; COSTA,MARIA ANGÉLICA PEREIRA DE CARVALHO. |
| The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L.) Merrill) cv. Pérola. Changes in benzylaminopurine (BAP) concentration, type of medium (liquid or solidified) and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%), for three minutes, followed by calcium hypochlorite (2%), for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1), 27 ± 2ºC. After eight weeks,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ananas comosus; Tissue culture; In vitro culture; In vitro clonal propagation. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000200005 |
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| Moura,Elisa Ferreira; Motoike,Sérgio Yoshimitsu. |
| The biotechnological techniques may help solve many problems of guava culture, such as the high perishability of fruits. Somatic embryogenesis can generate highly multiplicative cell cultures and with high regenerative potential, serving as basis for genetic transformation. The aim of this work was to obtain somatic embryogenesis of guava (Psidium guajava L.) cv. Paluma. Immature seeds were used, and they were inoculated in MS environment containing 400 mg L-1 of L-glutamine, 100 mg L-1 myo-inositol, 60 g L-1 sucrose, 100 mg L-1 ascorbic acid and supplemented with different types and concentrations of growth regulators. Embryogenic callus appeared after 37 days of culture in an environment containing 1.0 mg L-1 2.4-D + 2.0 mg L-1 2-ip, in 7% of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Growth regulators; Myrtaceae. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452009000200027 |
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| Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Junghans,Tatiana Góes; Jesus,Onildo Nunes de; Miranda,Diego; Otoni,Wagner Campos. |
| Abstract The propagation of many passion fruit species (Passiflora spp.) is done by seeds or sexual method, although asexual propagation, such stem cuttings, grafting or tissue culture is also possible and very useful in many cases. In this work, it was presented information on scientific and technological advances in sexual and asexual propagation of passion fruit, considering the different propagation methods and their applications. New demands for research and development activities are also related, given the wide range of passion fruit species and its potential for food, ornamental and medicinal use. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seed germination; Vegetative propagation; Cuttings; Grafting; Tissue culture. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000201002 |
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| Ledo,Ana da Silva; Lameira,Osmar Alves; Benbadis,Abdellatif Kemaleddine; Menezes,Ilmarina Campos de; Oliveira,Maria do Socorro Padilha de; Medeiros Filho,Sebastião. |
| The aim of this work was to study the morphogenetic responses of zygotic embryos of açai palm (Euterpe oleracea Mart.) submitted to several conditions of in vitro culture. Several research experiments were conducted, in laboratory, using vegetable material collected from açai palm plants at Embrapa Amazon Oriental, Belém-PA, Brazil. It was possible to verify the expression of a direct, repetitive and no-synchronized model of somatic embryogenesis in mature zygotic embryos cultivated in primary MS medium supplemented with 2,4-D (339.36 muM) and transferred to a secondary MS medium in the presence of NAA (0.537 muM) and 2iP (12.30 muM). The conversion of somatic embryos into seedlings was reached after 210 days with the transfer of the cultures to a third... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Açai palm; Growth regulators; Micropropagation; Tissue culture. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300004 |
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| Soriano,Leonardo; Tavano,Eveline Carla da Rocha; Behling,Maurel; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi. |
| Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L-1). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adventitious shoot; Citrus limonia; Tissue culture. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452012000200006 |
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| Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de. |
| This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031 |
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| PEREIRA,FERNANDO MENDES PEREIRA; USMAN,MUHAMMAD; MAYER,NEWTON ALEX; NACHTIGAL,JAIR COSTA; MAPHANGA,OSCAR RANNY MBONGENI; WILLEMSE,SALOMIE. |
| ABSTRACT Guava (Psidium guajava L.) can be propagated by seed, layering, air layering, grafting (budding or grafting), cuttings (root or shoot) or tissue culture. Propagation by seed is used for rootstock production and for raising populations for screening at early phases in the breeding programs. Vegetative propagation methods are used to clone selected genotypes from these programs and commercial orchards because it perpetuates all characteristics of each cultivar. This review addresses different methods that can be used to propagate guava, the methods commercially adopted and the progress obtained in recent years. There are several propagation technologies available, however, the adoption levels are rather different between producing countries. Needs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psidium guajava; Seeds; Grafting; Cutting; Air layering; Tissue culture. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401002 |
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| Ferreira,Mileide dos Santos; Moura,Érica Rodrigues de; Lino,Lucymeire Souza Morais; Amorim,Edson Perito; Santos-Serejo,Janay Almeida dos; Haddad,Fernando. |
| Abstract The banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ‘Prata-Anã’ resistant to Fusarium wilt by inducing somaclonal variation. ‘Prata-Anã’ stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biotechnology; Tissue culture; Musa sp.; Somaclonal variations; Fusarium wilt. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000600101 |
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| MAYER,NEWTON ALEX; BIANCHI,VALMOR JOÃO; FELDBERG,NELSON PIRES; MORINI,STEFANO. |
| ABSTRACT Nursery trees of stone fruits (Prunus spp.) are traditionally produced by union of two distinct genotypes - the rootstock and the scion - which, by grafting, form a composite plant that will be maintained throughout of all plant life. In Brazil, the rootstocks are predominantly seed propagated and therefore usually results in heterogeneous trees for vigor and edaphic adaptation. However, with advances in rootstock breeding programs that released cultivars and certification in several countries (notably in Europe), the system will come gradually evolving for vegetative propagation (cuttings and tissue culture) and use of seeds of selected rootstocks with specific characteristics and potted nursery trees production. For scion cultivar propagation,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prunus spp.; Seeds; Cutting; Grafting; Air layering; Tissue culture. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401001 |
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| Carvalho,Alexandre Alves de; Bertolucci,Suzan Kelly Vilela; Silva,Sâmia Torres; Pinto,José Eduardo Brasil Pereira. |
| ABSTRACT The aims of this study were to establish micropropagation for Chenopodium ambrosioides L. and analysis of volatile constituents in vitro as a function of different salt concentrations of MS medium and sucrose. The apical buds were established on MS medium added with 3% sucrose. The vegetative growth of nodal segments was tested in different salt concentrations of MS basic medium (0.25, 0.50, 1.00, 1.50, and 2.00) and of sucrose (5, 10, 20, 30, and 40 g L-1). Nodal and apical segments were tested in vertical and horizontal orientations. Different volumes of culture medium (20; 35, 50, 65, and 80 mL) were examined in the growth of nodal segments. Plants were acclimatized in three different substrates. The apical segments collected from matrices... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chenopodium ambrosioides L.; Medicinal plant; Tissue culture. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000400624 |
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| Rodrigues,Antonio Anderson de Jesus; Santos,Eder de Oliveira; Takane,Roberto Jun; Carvalho,Ana Cristina Portugal Pinto de. |
| ABSTRACT The technique of etiolation is widely used in the micropropagation of various plant species. The aim of this study was to evaluate growth and development in shoots of Cattleya labiata grown in vitro, as a function of growth regulators and the presence and absence of light. Stem segments, approximately ± 1.0 cm in height, were inoculated in test tubes containing 15.0 mL of MS culture medium supplemented with different concentrations of BAP (0.0, 2.0 and 4.0 mg L-1) and NAA (0.0, 1.0 and 2.0 mg L-1), and in different growth environments (growth room, darkness and a photoperiod of 16 hours) in a completely randomised design. At 150 days, the following were evaluated: a) number of etiolated shoots; b) number of nodes per etiolated shoot; c) length of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchidaceae; Tissue culture; Photoperiod; 6-benzylaminupurine; Naphthaleneacetic acid. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000200296 |
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| Registros recuperados: 97 | |
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