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Registros recuperados: 34
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INTERACCIÓN MICORRIZAS ARBUSCULARES-Trichoderma harzianum (Moniliaceae) Y EFECTOS SOBRE EL CRECIMIENTO DE Brachiaria decumbens (Poaceae) Acta biol.Colomb.
SOSA RODRÍGUEZ,TIFFANY; SÁNCHEZ NIEVES,JIMENA; MORALES GUTIÉRREZ,ESPERANZA; CRUZ CORTÉS,FERNANDO.
Se efectuó un ensayo en condiciones controladas utilizando hongos formadores de micorrizas arbusculares (HFMA) nativos, provenientes de un suelo rizosférico de Pennisetum clandestinum de la Universidad Nacional de Colombia (Bogotá), manteniéndolos en plantas de Brachiaria decumbens creciendo sobre sustrato arenoso suplementado con solución nutritiva. Se evaluaron diferentes tratamientos: plantas con inóculo de HFMA, plantas con Trichoderma harzianum, plantas con HFMA+T. harzianum y plantas control no inoculadas, con el fin de determinar las posibles interacciones entre dichos microorganismos, así como su efecto sobre el crecimiento de B. decumbens. La presencia de T. harzianum disminuyó la colonización radicular por HFMA, aunque no afectó la cantidad de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hongos formadores de micorrizas arbusculares (HFMA); Trichoderma harzianum; Rizósfera; Interacción.
Ano: 2006 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2006000100004
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Native isolates of Trichoderma harzianum inducting resistance to Zymoseptoria tritici onwheat plants Boletín de la Sociedad
Stocco,Marina C; Mansilla,Andrea Y; Mónaco,Cecilia I; Segarra,Carmen; Lampugnani,Gladys; Abramoff,Cecilia; Marchetti,María F; Kripelz,Natalia; Cordo,Cristina C; Consolo,Verónica F.
Trichoderma harzianum inducting resistance to Zymoseptoria tritici. Septoria tritici blotch is endemic in the wheat growing cultivated areas of Argentina, which impacts in the crop yield. One management strategy is to use biocontrol agents. The objective of this work was to determine the effectiveness of Trichoderma spp. isolated from soils of different growing regions, as antagonist and characterize the T. harzianum effect throughout the proteolytic activity of the serine protease in the wheat plant. To determine the antagonistic capacities of Trichoderma spp. against Zimoseptoria tritici, wheat plants were grown from seeds coated with each of the ninety isolated Trichoderma. Antagonism was assessed 21 days after inoculation by the capacity of each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Biocontrol; Wheat; Septoria tritici blotch; ISR; Serine protease.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722015000300002
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Biocontrol del moho foliar del tomate Cladosporium fulvum empleando cuatro hongos antagonistas Bragantia
Torres,Enrique; Iannacone,José; Gomez,Hilda.
El moho foliar Cladosporium fulvum (Cooke 1883) produce una enfermedad que ataca el cultivo de tomate en invernadero en el Perú. Como alternativa al control de este patógeno se probó la eficiencia bajo condiciones in vitro e invernadero de cuatro hongos antagonistas: Hansfordia pulvinata (Berk y Curt 1958), Trichoderma harzianum (Rifai 1969), T. viride (Persoon 1821) y T. virens (Miller, Giddens y Foster 1963). El cultivo y crecimiento de los hongos se realizó en Agar Sabouraud para Trichoderma spp. y en Agar Jugo V8 para H. pulvinata. El bioensayo empleado fue el de placas precolonizadas propuesto por Krauss. En condiciones in vitro se observó que a 24 ºC y 72 h, el crecimiento de T. harzianum fue estadísticamente diferente de las otras tres especies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cladosporium fulvum; Control biológico; Hansfordia pulvinata; Tomate; Trichoderma harzianum; Trichoderma viride; Trichoderma virens.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052008000100021
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Three tubulin genes of Trichoderma harzianum: alpha, beta and gamma BABT
Li,Min; Yang,Qian; Song,Jinzhu.
Three tubulin genes of Trichoderma harzianum were cloned followed genomic walking procedure. The tubulins showed high degree of amino acid homology with other fungal tubulins and were homologous with each other with 32 to 38% amino acid identity. Three measures for the degree of codon usage bias indicated the presence of bias in all the sequences, suggesting high expression levels in all the genes. Protein structures were modeled to provide the basis for understanding the tubulin's properties and its interactions with microtubule-associated proteins. Potential motifs were also postulated.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Tubulin; Codon usage bias; Motif.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400009
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Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3 BABT
Isil,Seyis; Nilufer,Aksoz.
In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were investigated. It was concluded that for maximum xylanase activity, incubation at 60°C in an enzyme incubation medium with pH 5 that contained 1 % xylan was appropriate. The stability studies showed that the enzyme was relatively stable in the pH range 3-7 and retained more than 50 % of its original activity after four months.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Xylan; Xylanase; Lignocellulosic material; Enzyme stability; Metal ions.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200004
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Production of spores of Trichoderma harzianum on sugar cane molasses and bagasse pith in solid state fermentation for biocontrol BABT
Rodríguez-León,Jose A.; Domenech,F.; León,M.; Méndez,T.; Rodríguez,D. E.; Pandey,Ashok.
Solid state fermentation was carried out for the production of spores from Trichoderma harzianum No 53 using sugar cane bagasse pith as solid matrix and sugar cane molasses as carbon and energy source. Different nitrogen sources such as urea, (NH4)2SO4 , NH4H2PO4 and (NH4)2HPO4 were added in the media to test their effect on spores production. Among these, urea was found most useful that resulted high no of spores (1x10(9)/gDM). The influence of temperature and initial moisture of the substrate was studied through a 2² experimental plan design. No statistical differences were found within the range of 30-35ºC and 60-70% for temperature and moisture respectively. The biotechnological parameters of the process were derived from the Oxygen Uptake Rate (OUR)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Solid state fermentation; Sugar cane pith and molasses; Biocontrol; Kinetics.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100010
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Biological control of Phytophthora root rot of avocato with microorganisms grown in organic mulches BJM
Costa,Jefferson L. da S.; Menge,John A.; Casale,Willian L..
Organic mulches colonized with microbial biocontrol agents, termed bioenhanced mulches, were tested for their ability to reduce Phytophthora root rot of avocado (Persea americana Mill.). Benomyl-resistant mutants of Gliocladium virens (KA 230-1) and Trichoderma harzianum (KA 159.2) isolated from suppressive soils and selected as efficient antagonists of P. cinnamomi were evaluated for their ability to colonize different mulches under controlled laboratory conditions. Sudangrass and a coarse yardwaste were found to be better substrates than a fine yardwaste, woodwaste or rice hulls for biocontrol agents propagules production. The most suitable conditions for colonization were an optimum temperature of 24°C, a moisture content of 20% for sudangrass and 30%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytophthora cinnamoni; Gliocladium virens; Trichoderma harzianum; Avocado.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400002
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Biological control of rice brown spot with native isolates of three Trichoderma species BJM
Khalili,Elham; Sadravi,Mehdi; Naeimi,Shahram; Khosravi,Vahid.
Brown spot caused by Bipolaris oryzae is an important rice disease in Southern coast of Caspian Sea, the major rice growing region in Iran. A total of 45 Trichoderma isolates were obtained from rice paddy fields in Golestan and Mazandaran provinces which belonged to Trichoderma harzianum, T. virens and T. atroviride species. Initially, they were screened against B. oryzae by antagonism tests including dual culture, volatile and nonvolatile metabolites and hyperparasitism. Results showed that Trichoderma isolates can significantly inhibit mycelium growth of pathogen in vitro by producing volatile and nonvolatile metabolites Light microscopic observations showed no evidence of mycoparasitic behaviour of the tested isolates of Trichoderma spp. such as coiling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rice brown spot; Biocontrol; Trichoderma harzianum; T. virens; T. atroviride.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100035
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Biocontrol potential of salinity tolerant mutants of Trichoderma harzianum against Fusarium oxysporum BJM
Mohamed,Hassan Abdel-Latif A.; Haggag,Wafaa Mohamed.
Exposing a wild-type culture of Trichoderma harzianum to gamma irradiation induced two stable salt-tolerant mutants (Th50M6 and Th50M11). Under saline conditions, both mutants greatly surpassed their wild type strain in growth rate, sporulation and biological proficiency against Fusarium oxysporum, the causal agent of tomato wilt disease. Tolerant T. harzianum mutants detained a capability to grow and convinced sporulation in growth media containing up to 69 mM NaCl. In comparison with their parent strain, characterization of both mutants confirmed that they have reinforced contents of proline and hydroxyproline, relatively higher sodium content compared to potassium, calcium or magnesium contents, higher level of total phenols. Electrophoretic analysis of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal metabolites; Mutagenesis; Gamma-irradiation; Salt stress; Trichoderma harzianum; Fusarium oxysporium.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200016
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Production and use of mutanase from Trichoderma harzianum for effective degradation of streptococcal mutans BJM
Wiater,Adrian; Szczodrak,Janusz; Pleszczynska,Malgorzata; Próchniak,Katarzyna.
Basic cultural parameters affecting mutanase production by Trichoderma harzianum F-340 in shaken flasks and aerated fermenter cultures have been standardized. The best medium for enzyme production was Mandels medium A with initial pH 5.3, supplemented with 0.3% mutan and 0.05% peptone and inoculated with 20% of the 72-h mycelium as inoculum. It was shown that mycelial mass, used in the culture medium as a sole carbon source, induced mutanase synthesis and could be utilized as an inexpensive and easily available substitute for bacterial mutan. Application of optimized medium and cultural conditions enabled us to obtain a high mutanase yield (0.6-0.7 U/mL, 2.0-2.5 U/mg protein) in a short period of time (3-5 days), which was much higher than the best...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Mutanase; Mutan.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200008
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The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens BJM
Singh,Rashmi; Maurya,Sudarshan; Upadhyay,Ram Sanmukh.
Abstract The antagonistic potential of Trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of Sclerotium rolfsii, the causal agent of chickpea collar rot. Trichoderma harzianum-1432 (42.2%) and Trichoderma atroviride (40.3%) were found to be strong antagonists. To enhance their antagonistic potential, mutagenesis of these two selected strains was performed. Two mutants, Th-m1 and T. atroviride m1, were found to be more effective than their parent strains. The enzymatic activities of the selected parent and mutant strains were assayed, and although both mutants were found to have enhanced enzymatic activities compared to their respective parent strains, Th-m1 possessed the maximum cellulase (5.69 U/mL) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Collar rot; Trichoderma harzianum; Sclerotium rolfsii.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100010
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Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse BJM
Rezende,Maria Inês; Barbosa,Aneli de Melo; Vasconcelos,Ana Flora Dalberto; Endo,Asae Sakurada.
Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Xylanase; Solid state fermentation; Sugarcane bagasse.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014
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Chemical and biological control of Sclerotinia stem rot in the soybean crop Ciência Rural
Sumida,Ciro Hideki; Canteri,Marcelo Giovanetti; Peitl,Douglas Casaroto; Tibolla,Fabiana; Orsini,Idenize Pedrina; Araújo,Felipe André; Chagas,Débora Fonseca; Calvos,Natália Sanches.
It was evaluated the effect of fungicides and the microbial control agent Trichoderma harzianum on the inhibition of the carpogenic and ascospore germination of Sclerotinia sclerotiorum. This study also evaluated the chemical, fungicidal and microbial control of white mold or Sclerotinia stem rot of soybean in the field. Three experiments were conducted, as follows: 1) inhibition of carpogenic germination of sclerotia, 2) inhibition of ascospore germination, and 3) control of Sclerotinia stem rot in a soybean crop under field conditions. The treatments evaluated were fluazinam, procymidone, iprodione, thiophanate-methyl, carbendazim, benzalkonium chloride + fluazinam, and T. harzianum. Procymidone resulted in an inhibition of 13.5% and benzalkonium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Trichoderma harzianum; Apothecium; Ascospore; Sclerotium; Soybean..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500760
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Inoculação de sementes de milho utilizando o Trichoderma harzianum como promotor de crescimento Ciência e Agrotecnologia
Resende,Maria de Lourdes; Oliveira,João Almir de; Guimarães,Renato Mendes; Von Pinho,Renzo Garcia; Vieira,Antônio Rodrigues.
Conduziu-se este trabalho com o objetivo de estudar o efeito do fungo Trichoderma harzianum como promotor de crescimento de sementes de milho tratadas com diferentes fungicidas. O experimento foi realizado em vasos em casa-de-vegetação, adotando o delineamento experimental inteiramente casualizado em esquema fatorial (3x2), utilizando os tratamentos com fungicidas: Captan®, Maxim® e testemunha e inoculação (com e sem inóculo) em quatro repetições. As características agronômicas avaliadas foram: altura de planta, peso da matéria seca da parte aérea e de raízes e também a técnica de isolamento do fungo nas raízes aos 45 dias após semeadura. Observou-se diferença significativa somente para o fator inóculo na característica peso de matéria seca de raízes....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Promotor; Crescimento; Milho.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000400010
Registros recuperados: 34
Primeira ... 12 ... Última
 

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