SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Bittencourt,Achiléa; Silva,Nancy; Straatmann,Andréa; Nunes,Victor Luiz Correia; Follador,Ivonise; Badaró,Roberto.

Post-kala-azar dermal leishmaniasis (PKDL) is rarely reported in South America. In spite of the fact that there are many reports about the association of visceral leishmaniasis and AIDS, PKDL is very uncommon in HIV-positive patients, and so far only four cases have been documented in the literature. We present another case with unusual clinicopathological aspects. The patient, a 28-year-old male, from Salvador, Bahia (an endemic area) presented with clinical manifestations of visceral leishmaniasis three years after the diagnosis of AIDS. During treatment for visceral leishmaniasis he developed disseminated miliary papules. Microscopically, the skin biopsy showed a "saw-tooth" pattern with a lichenoid mononuclear infiltrate simulating lichen planus. The...

Increased levels of IL-10, IL-12, and IFN- in patients with visceral leishmaniasis

Palavras-chave: Visceral leishmaniasis; Post-kala-azar dermal leishmaniasis; AIDS.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000600008

Khoshdel,A.; Alborzi,A.; Rosouli,M.; Taheri,E.; Kiany,S.; Javadian,M.H..

Visceral leishmaniasis remains a serious public health problem in developing countries. Cytokines have a crucial role in the pathogenesis of this disease. We evaluated plasma levels of IL-10, IL-12 and IFN- in 32 patients with active visceral lieshmaniasis, in 29 siblings of the patients and in 23 normal individuals in an endemic area to look for correlations between the clinical outcome of infection and the plasma cytokine levels. An analysis was made with a skin test and a quantitative sandwich enzyme immunoassay. Data were analyzed with the Mann Whitney test and the Kruskal Wallis test. The cytokine levels were significantly higher in the patients than in the control groups. We concluded that normalization of the plasma level of IFN- can serve as a...

Are opossums a relevant factor associated with asymptomatic Leishmania infection in the outskirts of the largest Brazilian cities?

Palavras-chave: Visceral leishmaniasis; Interleukin; Interferon.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100010

Carranza-Tamayo,César Omar; Werneck,Guilherme Loureiro; Romero,Gustavo Adolfo Sierra.

Abstract A population survey was conducted to explore the prevalence and factors associated with Leishmania infection in the Fercal region of the Federal District. The Fercal region is a group of neighborhoods in Brasília in which the first cases of visceral leishmaniasis were described. Leishmania infection was established by a positive leishmanin test. Although other tests were performed in the study (an immunochromatographic assay (Kalazar detect®) and a molecular assay), only the leishmanin skin test provided sufficient results for the measurement of the disease prevalence. Data on the epidemiological, clinical and environmental characteristics of individuals were collected along with the diagnostic tests. After sampling and enrollment, seven hundred...

Congenitally transmitted visceral leishmaniasis: report of two brazilian human cases

Palavras-chave: Visceral leishmaniasis; Brazil; Didelphis albiventris; Leishmanin.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200119

Mescouto-Borges,Myrlena Regina Machado; Maués,Érika; Costa,Dorcas Lamounier; Pranchevicius,Maria Cristina da Silva; Romero,Gustavo Adolfo Sierra.

Visceral leishmaniasis is a relevant public health problem worldwide. Most of the reported cases in Latin America are from Brazil. Herein we report two human cases of congenitally transmitted visceral leishmaniasis in two patients who developed symptoms during pregnancy. The diagnosis was made by visual examination of Leishmania parasites in bone marrow aspirates of the mothers and by detecting parasite kDNA in bone marrow samples of the newborn children using polymerase chain reaction.

Clinical presentation and renal evaluation of human visceral leishmaniasis (kala-azar): a retrospective study of 57 patients in Brazil

Palavras-chave: Congenital transmission; Visceral leishmaniasis; Leishmania infantum; Pregnancy.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200024

Daher,Elizabeth F.; Evangelista,Leandro F.; Silva Júnior,Geraldo B.; Lima,Rafael S.A.; Aragão,Eveline B.; Arruda,Germana A.J.C.; Galeano,Noemi M.F.; Mota,Rosa M.S.; Oliveira,Rodrigo A.; Silva,Sônia L..

Visceral leishmaniasis is an endemic disease caused by various species of Leishmania. We made a retrospective study of 57 consecutive patients with visceral leishmaniasis in Brazil. Patients with visceral leishmaniasis were identified using the registries of the São José Infectious Diseases Hospital. The sample was divided into two groups: patients with serum creatinine (Scr) <1.3mg/dL and Scr > 1.3mg/dL. We compared these two groups for differences in clinical manifestations and laboratory features. Patients' mean age was 28 ± 18 years old; 74% were male. The main clinical symptoms and signs presented in the initial evaluation were: fever (97%), splenomegaly (96.4%), weight loss (95.5%), pallor (93.6%), cough (89.7%), hepatomegaly (87.2%), asthenia...

Monoclonal gammopathy associated with visceral leishmaniasis

Palavras-chave: Visceral leishmaniasis; Kala-azar; Symptoms; Acute renal failure.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400015

Sharma,Vishal; Agarwal,Mukul P; Giri,Subhash.

Monoclonal gammopathy can accompany diverse conditions and is usually benign. It should be distinguished from monoclonal gammopathy of undetermined significance (MGUS) which can rarely turn malignant. Visceral leishmaniasis has only rarely been associated with monoclonal gammopathy. We describe the case of a 55-year-old male who had monoclonal gammopathy associated with visceral leishmanisais, which reversed with stibogluconate therapy.

Post-kala-azar dermal leishmaniasis associated with AIDS

Palavras-chave: Visceral leishmaniasis; Monoclonal gammopathy.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300018

Bittencourt,Achiléa; Silva,Nancy; Straatmann,Andréa; Nunes,Victor Luiz Correia; Follador,Ivonise; Badaró,Roberto.

Kidney involvement in leishmaniasis — a review

Palavras-chave: Visceral leishmaniasis; Post-kala-azar dermal leishmaniasis; AIDS.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300009

Silva Junior,Geraldo Bezerra da; Barros,Elvino José Guardão; Daher,Elizabeth De Francesco.

Leishmaniasis is an infectious disease caused by protozoa of the genus Leishmania transmitted by insects of the genus Lutzomyia sp. or Phlebotomus sp. The main syndromes are cutaneous leishmaniasis, mucocutaneous leishmaniasis, visceral leishmaniasis (kala-azar) and post-kala-azar dermal leishmaniasis. This article reviews kidney involvement in cutaneous and visceral leishmaniasis, highlighting the aspects of their pathophysiology, clinical manifestations, histopathological findings, outcome and treatment.

Detection of specific IgE antibodies in parasite diseases

Palavras-chave: Visceral leishmaniasis; American cutaneous leishmaniasis; Kala-azar; Kidney disease.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400434

Souza-Atta,M.L.B.; Araújo,M.I.; D'Oliveira Júnior,A.; Ribeiro-de-Jesus,A.; Almeida,R.P.; Atta,A.M.; Carvalho,E.M..

Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used....

Serum cytokine profile in the subclinical form of visceral leishmaniasis

Palavras-chave: IgE antibodies; Immunoassays; Schistosomiasis mansoni; Visceral leishmaniasis; Kala-azar.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008

Gama,M.E.A.; Costa,J.M.L.; Pereira,J.C.R.; Gomes,C.M.C.; Corbett,C.E.P..

The factors determining the development or not of visceral leishmaniasis (VL) have not been completely identified, but a Leishmania-specific cellular immune response seems to play a fundamental role in the final control of infection. Few studies are available regarding the production of cytokines in the subclinical form of VL, with only the production of IFN-g and TNF-a known. The aim of the present study was to identify immunological markers for the oligosymptomatic or subclinical form of VL. A prospective cohort study was conducted on 784 children aged 0 to 5 years from an endemic area in the State of Maranhão, Brazil, between January 1998 and December 2001. During 30 consecutive months of follow-up, 33 children developed the oligosymptomatic form of the...

CD4+ T cells participate in the nephropathy of canine visceral leishmaniasis

Palavras-chave: Visceral leishmaniasis; Cytokine levels; Immunological aspects; Subclinical form; Oligosymptomatic form.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100018

Costa,F.A.L.; Guerra,J.L.; Silva,S.M.M.S.; Klein,R.P.; Mendonça,I.L.; Goto,H..

Renal involvement in visceral leishmaniasis (VL) is very frequent. The renal lesions of humans and dogs are similar but their pathogenesis has not been clearly elucidated. There is growing evidence that the cellular immune response is involved in the pathogenesis of immunologically mediated glomerulonephritis. Since T cells could participate in the pathogenesis of nephropathy, in the present study we investigated the possible involvement of CD4+ and CD8+ T cells in the nephropathy of canine VL. Six dogs naturally infected with Leishmania (Leishmania) chagasi from the endemic area in the Northeast of Brazil, the town of Teresina in the State of Piauí, were studied. An expressive inflammatory infiltrate of CD4+ T cells both in glomeruli and in interstitium...

Anti-leishmania antibodies in cerebrospinal fluid from dogs with visceral leishmaniasis

Palavras-chave: Visceral leishmaniasis; Dog; Leishmania (Leishmania) chagasi; Nephropathy; Immunoglobulin; T cells.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200009

Lima,V.M.F.; Gonçalves,M.E.; Ikeda,F.A.; Luvizotto,M.C.R.; Feitosa,M.M..

Visceral leishmaniasis in Brazil is caused by Leishmania (Leishmania) chagasi and the dog is its most important reservoir. The clinical features in dogs include loss of weight, lymphadenopathy, renal failure, skin lesions, fever, hypergammaglobulinemia, hepatosplenomegaly, anemia, and, rarely, neurological symptoms. Most infected animals develop active disease, characterized by high anti-leishmania antibody titers and depressed lymphoproliferative ability. Antibody production is not primarily important for protection but might be involved in the pathogenesis of tissue lesions. An ELISA test was used to determine if there is an association between neurological symptoms and the presence of anti-L. chagasi antibodies in cerebrospinal fluid (CSF). Thirty serum...

Immunity and immunosuppression in experimental visceral leishmaniasis

Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Cerebrospinal fluid; Dogs; Antibodies.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400010

Goto,H.; Lindoso,J.A.L..

Leishmaniasis is a disease caused by protozoa of the genus Leishmania, and visceral leishmaniasis is a form in which the inner organs are affected. Since knowledge about immunity in experimental visceral leishmaniasis is poor, we present here a review on immunity and immunosuppression in experimental visceral leishmaniasis in mouse and hamster models. We show the complexity of the mechanisms involved and differences when compared with the cutaneous form of leishmaniasis. Resistance in visceral leishmaniasis involves both CD4+ and CD8+ T cells, and interleukin (IL)-2, interferon (IFN)- gamma, and IL-12, the latter in a mechanism independent of IFN- gamma and linked to transforming growth factor (TGF)-ß production. Susceptibility involves IL-10 but not IL-4,...

Detection of immunoglobulin G in the lung and liver of hamsters with visceral leishmaniasis

Palavras-chave: Visceral leishmaniasis; Immunosuppression; T cell; Cytokines; Mice; Hamster.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400020

Mathias,R.; Costa,F.A.L.; Goto,H..

Several organs are affected in visceral leishmaniasis, not only those rich in mononuclear phagocytes. Hypergammaglobulinemia occurs during visceral leishmaniasis; anti-Leishmania antibodies are not primarily important for protection but might be involved in the pathogenesis of tissue lesions. The glomerulonephritis occurring in visceral leishmaniasis has been attributed to immune complex deposition but in other organs the mechanism has not been studied. In the current study we demonstrated the presence of IgG in the lung and liver of hamsters with visceral leishmaniasis. Hamsters were injected intraperitoneally with 2 x 10(7) amastigotes of Leishmania (Leishmania) chagasi and the presence of IgG in the liver and lung was evaluated at 7, 15, 30, 45, 80 and...

Leishmania (Leishmania) chagasi-infected mice as a model for the study of glomerular lesions in visceral leishmaniasis

Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Immunopathology; Immunoglobulin G; Liver; Lung.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400015

Prianti,M.G.; Yokoo,M.; Saldanha,L.C.B.; Costa,F.A.L.; Goto,H..

Renal involvement in visceral leishmaniasis (VL) is very frequent but the pathogenesis of this nephropathy is poorly understood. In previous studies using dogs with VL we have detected new immunopathological elements in the glomeruli such as T cells and adhesion molecules. Although Leishmania (Leishmania) chagasi-infected dogs and hamsters are considered to be good models for VL, their use is limited for immunopathologic studies. The use of isogenic mouse strains susceptible to L. (L.) chagasi infection was an alternative but, on the other hand, the renal lesions of these animals have not yet been characterized. Thus, our purpose in the present study was to characterize mice infected with L. (L.) chagasi as a suitable model to study VL nephropathy. Kidney...

Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli

Palavras-chave: Visceral leishmaniasis; Glomerulonephritis; Mice; Model; Leishmania (Leishmania) chagasi.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600011

BJM

Vaz,Michelle Rossana Ferreira; França,Ricardo Luiz Soares de; Andrade,Sirtys Santos Lessa de; Sousa Junior,Francisco Canindé de; Santos,Everaldo Silvino dos; Martins,Daniella Regina Arantes; Macedo,Gorete Ribeiro de.

With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of...

Parasite load, iNOS and cytokine profiles, and histopathological aspects of Leishmania infantum infection in dogs with different clinical presentations

Palavras-chave: Cultivation; Recombinant protein; Visceral leishmaniasis; Escherichia coli.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021

Ciência Rural

Vasconcelos,Tassia Cristina Bello de; Bruno,Sávio Freire; Miranda,Luisa Helena Monteiro de; Conceição-Silva,Fátima; Belo,Vinícius Silva; Figueiredo,Fabiano Borges.

ABSTRACT: Visceral leishmaniasis (VL) is a zoonotic disease with a canine urban reservoir in South America. Dogs from an endemic area within Brazil, which were naturally infected with Leishmania infantum, and those presenting severe clinical (SC), mild, or no clinical (MNC) disease, were evaluated. Parasite load, histopathology, and cytokine and iNOS mRNA expressions were assessed in the spleen and liver in order to determine the potential markers for disease susceptibility or resistance. As a result, dogs with both SC and MNC had high parasite loads; IFN-γ was the most expressive cytokine in both organs, along with IL-6 and IL-4 being detected in the spleen and liver, and IL-10 only in liver. The hepatic tissue presented higher medians for IFN-γ and...

High frequency of visceral leishmaniasis in dogs under veterinary clinical care in an intense transmission area in the state of Tocantins, Brazil

Palavras-chave: Visceral leishmaniasis; Immunopathology; Canine infection; Histopathology; Canine cytokines.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001000501

Ciência Rural

Santos,Helcileia Dias; Galvão,Samara Rocha; Dias,Francisca Elda Ferreira; Ribeiro,Taiã Mairon Peixoto; Negreiros Filho,Osmar; Sousa,Sebastiana Adriana Pereira; Minharro,Silvia.

ABSTRACT: A direct search for parasites were used as the diagnostic test to determine the frequency of Leishmania spp. infection in dogs ( Canis lupus familiaris ) under veterinary clinical care in the city of Araguaína, Tocantins, Brazil. For this approach, lymph node cell samples were collected using needle aspiration from 649 dogs of different breeds and ages. Two hundred and sixty four (40.7%) dogs tested positive for amastigote forms of Leishmania spp. Furthermore, 202 (76.5%) dogs that tested positive showed some clinical sign of disease, while 62 (28.4%) dogs were asymptomatic. Dogs <2 years old or those that lived alongside poultry species in peri-domicile areas had a greater chance of infection (P<0.05). Our results revealed the importance...

Leishmania chagasi in dogs from the city of Jaciara, Mato Grosso, Brazil

Palavras-chave: Visceral leishmaniasis; Cytodiagnosis; Risk factors; Prevention and control.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300501

Ciência Rural

Lazari,Patrícia; Almeida,Arleana do Bom Parto Ferreira de; Dutra,Valéria; Nakazato,Luciano; Dias,Álvaro Felipe de Lima Ruy; Brito,Veruska Nogueira de; Oliveira,Cenita Maria; Sousa,Valéria Régia Franco.

ABSTRACT: The aim of this study was to identify the Leishmania species in Jaciara dogs; visceral leishmaniasis (VL) is endemic in the county of Jaciara. A comparison among the PCR results for the spleen, bone marrow, skin and blood of 101 dogs with VL-reactive serum were evaluated. Spleen tissue showed the highest detection percentage, followed by bone marrow. In 97 (96.04%) dogs, the Leishmania DNA detected was confirmed as L. chagasi, emphasizing the importance of aetiological identifications in sympatric areas with other trypanosomatids.