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| Registros recuperados: 9.178 | |
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| Solano Solis,Jaime; Morales Ulloa,Daniza; Anabalón Rodríguez,Leonardo. |
| Chile is considered to be a sub-center of origin for the cultivated potato, with native and introduced genetic material coexisting in the country. Thus, the different varieties present in Chiloe Island are characterized by a rich diversity of forms, sizes, colours and phenological characteristics. In the present work, the level of polymorphism and the genetic relationship were studied by means of molecular markers using the amplified fragment length polymorphism (AFLP) technique and twenty-seven morphological characters. Twenty varieties of potatoes from the Chiloe Island were analyzed. The commercial variety Desirée and one specie from the Etuberosa series, Solanum fernandezianum, collected in the Juan Fernandez Island were included as controls. A... |
| Tipo: Journal article |
Palavras-chave: Cluster analysis; Molecular markers; Solanum tuberosum ssp. tuberosum. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300011 |
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| Abdullah,Ruslan; Zainal,Alizah; Yew Heng,Wee; Chui Li,Leaw; Chee Beng,Yeap; Mei Phing,Lee; Abdullah Sirajuddin,Salwa; Soo Ping,Winnie Yap; Lourdes Joseph,Juanita; Azma Jusoh,Siti. |
| Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation... |
| Tipo: Journal article |
Palavras-chave: Genetic transformation studies; Immature embryos; In vitro culture; Oil palm; Plant regeneration. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006 |
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| Yongzhong,Lu; Zhang,Xuecheng. |
| In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin b subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the... |
| Tipo: Journal article |
Palavras-chave: CpcB gene; Deletion analysis; Light intensity; Synechococcus; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100010 |
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| Xu,Xinmiao; Shen,Ying; Chen,Jiacheng. |
| Background CO2 emission, water pollution and petroleum shortage are the issues coming with the development of industry. A cost effective system was constructed to fix the CO2 in flue gas (15% CO2), remove nitrogen and phosphorus from manure wastewater and produce biofuels at the same time. The significant cultivation conditions were selected by Plackett-Burman design, and then optimized with central composite design. Results Optimum culture condition was predicted at light intensity of 238 µmol·m- 2·s- 1, TN of 152 mg·L- 1, inoculum density of 0.3 g·L- 1, under which the measured CO2 fixation rate, total nitrogen and phosphorus removing rate, and lipid content were 638.13 mg·L- 1·d- 1, 88.16%, 73.98% and 11.9%, respectively. The lipid content was then... |
| Tipo: Journal article |
Palavras-chave: CO2 fixation rate; Flue gas; Lipid content; Scenedesmus dimorphus; Wastewater. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100009 |
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| Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian . |
| This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for... |
| Tipo: Journal article |
Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014 |
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| Sarwat,Maryam; Singh Negi,Madan; Lakshmikumaran,Malathi; Kumar Tyagi,Akhilesh; Das,Sandip; Shankar Srivastava,Prem. |
| For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). |
| Tipo: Journal article |
Palavras-chave: DNA extraction; Medicinal plants; Molecular markers; Terminalia species. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011 |
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| Peñaloza,Patricia; Ramirez-Rosales,Gerardo; McDonald,Miller B; Bennett,Mark A.. |
| High seed quality is essential for optimum stand establishment in lettuce. As a result, it is necessary to have seed vigour tests that permit rapid, objective and accurate evaluation of seed quality. This study evaluated physical and physiological seed quality components of four seed lots of six lettuce varieties obtained from a commercial company. Seeds were evaluated for seedling emergence under greenhouse conditions, standard germination, seed physical aspects, the Saturated Salt Accelerated Aging (SSAA) test and the Seed Vigour Imaging System (SVIS). Results indicated that large-seeded lettuce varieties had higher percentage germination, higher SSAA values, higher SVIS index and more rapid and uniform greenhouse emergence. Black-seeded lettuce... |
| Tipo: Journal article |
Palavras-chave: Vegetables; Deterioration; Seedling; Performance. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300010 |
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| Wang,Ping; Nong,Xu-Hua; Ge,Jie-Hong. |
| Nonylphenol ethoxylates (NPEOs), which are widely used for industrial and domestic purposes, exert adverse effects on wildlife after being used and discharged into the environment. However, their ultimate biodegradability and biodegradation pathway remains unclear. In this study, the aerobic degradability of nonylphenol ethoxylates (NPEOs) by the acclimated microorganisms in active sludge was examined using shaking-flask tests. The degradation of benzene rings in NPEOs was determined using UV spectroscopy and high performance liquid chromatography (HPLC). Results showed that more than 80% of benzene rings were removed after 8-10 days of degradation, and the majority of NPEOs were also removed after 9 days of degradation, indicating NPEOs and the benzene... |
| Tipo: Journal article |
Palavras-chave: Aerobic biodegradation; Degradation pathway; ESI-MS; HPLC; Nonylphenol ethoxylates. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400001 |
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| Tsay,Hsin-Sheng; Ho,Hui-Min; Gupta,Sushim K; Wang,Chang-Sheng; Chen,Pei-Tsu; Chen,Emily Chin-Fun. |
| In the present study, a novel plant transformation system for Doritaenopsis and Phalaenopsis has been developed. The pollen-mediated activation tagging system was established by artificial pollination. The pollens, co-cultured with Agrobacterium tumefaciens strain EHA105 harbouring an activation tagging vector (pTAG-8), were used for pollination. In order to optimize the transformation efficiency, several factors (concentration of A. tumefaciens, concentration of acetosyringone during co-cultivation and the duration of co-cultivation) known to influence Agrobacterium-mediated DNA transfer were examined. A concentration of 0.5-1 x 10(8) CFU/ml for A. tumefaciens, 0.1 mM acetosyringone, and 6 hrs of co-culture period were found to be the optimal condition... |
| Tipo: Journal article |
Palavras-chave: Activation tagging; Agrobacterium tumefaciens; Doritaenopsis cultivars; Phalaenopsis amabilis; Transformation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400009 |
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| Alarcón,Evelyn; Decap,Jacqueline; Vidal,Gladys. |
| Kraft mills are responsible for the massive discharge of highly polluted effluents, and new bleaching processes (i.e. Total Chlorine Free (TCF)) is presented as a feasible option to reduce this environmental impact. However, increased TCF pulp production is accompanied by an increase in chelate use. The most commonly used chelates, ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DPTA), are considered to be relatively persistent substances in water treatment plants, and consequently environmentally critical compounds. The purpose of this work is to investigate DPTA behaviour in an anaerobic system. An Anaerobic Filter (AF) was operated with three different DPTA load rates (LR DPTA = 0.07 - 0.28 gDPTA/L×d), and the... |
| Tipo: Journal article |
Palavras-chave: Anaerobic biodegradation; DTPA; Kraft mill effluent. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300011 |
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| Lopez,Jose V; Ledger,Angela; Santiago-Vázquez,Lory Z; Pop,Mihai; Sommer,Dan D; Ranzer,Llanie K; Feldman,Robert A; Russell,G. Kerr. |
| Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non-“housekeeping” genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila... |
| Tipo: Journal article |
Palavras-chave: CDNA; Erythropodium caribaeorum; EST; Gorgonia ventalina; Gorgonian; Sea fan. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100008 |
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| Donoso-Bravo,Andrés; Carballa,Marta; Ruiz- Filippi,Gonzalo; Chamy,Rolando. |
| In this work, an anaerobic sequencing batch reactor (ASBR) was operated for 8 months to treat low strength sewage with high suspended organic matter content. Three phases of operation with increasing organic loading rates (OLR) were performed: 0.4 kg COD/m³ x d (phase I), 0 .8 kg COD/m³ x d (phase II) and 1.2 kg COD/m³ x d (phase III). Adequate stability parameters (pH, total alkalinity) were obtained through all three experimental phases. During phases I and II, the removal efficiencies of organic matter (expressed as total chemical oxygen demand (COD) and total suspended solids ranged between 50-60%. However, these values decreased to 15-25% in phase III. In addition, a non-complex model, including hydrolysis, acidogenesis and methanogenesis, was applied... |
| Tipo: Journal article |
Palavras-chave: Acidogenesis; Anaerobic treatment; Hydrolysis; Low strength wastewater; Modeling. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300013 |
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| Osorio,Mayra; Gámez,Elizabeth; Molina,Sandy; Infante,Diógenes. |
| Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in micropropagated plants from somatic embryos of Venezuelan native clone 56. Results: Three markers were used: ISTR, AFLP and SSR, finding that ISTR showed the highest polymorphism among individuals tested. With AFLP a high similarity between the evaluated individuals was observed and with SSR total monomorphism was seen. Using cluster analysis it was found that individuals from an embryo labeled as fasciated at the... |
| Tipo: Journal article |
Palavras-chave: AFLP; Genetic stability; ISTR; SSR; UPGMA. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400003 |
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| Registros recuperados: 9.178 | |
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