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| Registros recuperados: 49 | |
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| Genard, Bertrand; Larouche, Olivier; Nicolas, Jean-louis; Miner, Philippe; Beaudin, Marie-lou; Tremblay, Rejean. |
| The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival... |
| Tipo: Text |
Palavras-chave: Probiotics; Hatchery; Bacterial challenge; Larval development; Pecten maximus; Phaeobacter gallaeciensis; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00213/32429/30895.pdf |
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| Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre. |
| Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence... |
| Tipo: Text |
Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy. |
| Ano: 2006 |
URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf |
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| Le Deuff, Rose-marie; Nicolas, Jean-louis; Renault, Tristan; Cochennec, Nathalie. |
| A herpes-like virus which was associated with mortalities amongst hatchery-reared larvae of Pacific oysters, Crassostrea gigas. was observed for the first time in France during the summer 1991 (Nicolas et al.. 1992). Since this first report, we have noted further outbreaks of the disease in the summers of 1992 and 1993 in hatcheries on the western coasts of France in which a similar virus was associated with moribund larvae. Moreover, in the summer 1991, Hine et al. (1992) described a herpes-like virus responsible for larval mortality of hatchery reared C. gigas, in New Zealand. Ail these viruses are c10sely related with respect to their size and morphology and to their apparent cellular tropism for fibroblastic cells. |
| Tipo: Text |
Palavras-chave: Transmission; Herpes like virus; Crassostrea gigas; Oysters. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-2887.pdf |
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| Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie. |
| in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the... |
| Tipo: Text |
Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf |
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| Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique. |
| Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V... |
| Tipo: Text |
Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf |
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| Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis. |
| This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15. Bacterial... |
| Tipo: Text |
Palavras-chave: RAS; Bivalve larvae; Microbiota; Pyrosequencing; Hatchery. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00346/45691/45311.pdf |
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| Registros recuperados: 49 | |
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