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DIPNET - A European project to evaluate disease interactions and pathogen exchange between farmed and wild aquatic animal population fish, shellfish and crustaceans ArchiMer
Miossec, Laurence; Garseth, A. H.; Midtlyng, P. J.; Raynard, R.; Peeler, E.; De Bals, I..
DIPNET (Disease Interaction and Pathogen exchange Network) is a co-ordination action funded under the UE Framework programme 6 priority 8 Scientific Support to Policy (SSP). The principal objective of DIPNET is to increase scientific knowledge of the potential transfer of pathogens and diseases between wild and farmed fish and shellfish populations. DIPNET started in October 2004 and is a 2 year project. The project is organized in four work packages. Work package (WP) 1 is focused on a literature review of disease interactions and pathogen exchange between farm and wild aquatic animals. This analysis includes both published and unpublished literature. The work covers a wide range of habitats and species within Europe and, where relevant, world-wide. WP1...
Tipo: Text Palavras-chave: DIPNET; Crustaceans; Shellfish; Fish; Epidemiology; Risk assessment; Diseases; Transfer of pathogens.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3325.pdf
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Distribution, prevalence, and genetic analysis of Panulirus argus virus 1 (PaV1) from the Caribbean Sea ArchiMer
Moss, Jessica; Behringer, Donald; Shields, Jeffrey D.; Baeza, Antonio; Aguilar-perera, Alfonso; Bush, Phillippe G.; Dromer, Clement; Herrera-moreno, Alejandro; Gittens, Lester; Matthews, Thomas R.; Mccord, Michael R.; Schaerer, Michelle T.; Reynal, Lionel; Truelove, Nathanial; Butler, Mark J..
The pathogenic virus Panulirus argus virus 1 (PaV1) was first discovered in Caribbean spiny lobsters Panulirus argus from the Florida Keys (USA) in 1999 and has since been reported in Belize, Mexico, and Cuba; its distribution in the wider Caribbean is unknown. We collected tissue samples from adult spiny lobsters from 30 locations in 14 countries bordering the Caribbean Sea and used molecular diagnostics to assay for the presence of PaV1. PaV1 occurred primarily in the northern areas of the Caribbean, where its prevalence was highest. The virus was not found in lobsters from the southeastern Caribbean, and its prevalence was lowest in the southwestern Caribbean. DNA sequence analysis was performed on a fragment of the viral DNA to examine the genetic...
Tipo: Text Palavras-chave: Spiny lobster; Disease; Epidemiology; Connectivity.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00144/25503/23698.pdf
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Comparative analysis of Vibrio splendidus-related strains isolated during Crassostrea gigas mortality events ArchiMer
Le Roux, Frédérique; Gay, Melanie; Lambert, Christian; Waechter, Magali; Poubalanne, Saravanne; Chollet, Bruno; Nicolas, Jean-louis; Berthe, Franck.
French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar H strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast...
Tipo: Text Palavras-chave: Vibrio splendidus; Virulence; Epidemiology; PCR RFLP; Oyster mortality.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-565.pdf
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Relance de l'huître plate. Rapport d'avancement des travaux. Année 1992 ArchiMer
Martin, Anne-genevieve.
A revival program for the European flat oyster Ostrea edulis was initiated in Brittany in 1984 and funded by regional and national agencies. Since then, the research programs were carried out and the 1992 results are presented in this report. Disease status and spat settlement trends are described within each breeding area as well as advances in management practices and genetic research.
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiologie; Pathologie; Génétique; Echantillonnage; Elevage; Shellfish; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiology; Pathology; Genetic; Sampling; Breeding.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00427/53823/54814.pdf
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Relance de l'huître plate, Ostrea edulis. Bilan des travaux réalisés en 1993 ArchiMer
Martin, Anne-genevieve.
A revival program for the European flat oyster Ostrea edulis was initiated in Brittany in 1984 and funded by regional and national agencies. Since then, the research programs were carried out and the 1993 results are presented in this report. Disease status and spat settlement trends are described within each breeding area as weil as advances in genetic research.
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiologie; Pathologie; Génétique; Echantillonnage; Elevage; Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiology; Pathology; Genetic; Sampling; Breeding.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00427/53822/54813.pdf
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Presence of Salmonella in shellfish in the Thau lagoon ArchiMer
Angeli, J.
After a general presentation of the Thau lagoon together with its geographic location, socio-economic activities linked to shellfish culture and fishing, this paper investigates the health conditions of the shellfish harvesting areas. I he influence of heavy rainfall following a prolonged drought period is underlined as a determining factor of the momentary bacterial pollution in the harvesting areas, leading to a suspension of sales based on analyses relying solely on the detection of faecal coliforms. As of 1989, bacteriological monitoring was reorganized in the form of a data collection network taking into consideration environmental assessments of water quality as well as shellfish hygiene in order to protect consumer health. Its extension to the...
Tipo: Text Palavras-chave: Salmonella; Bivalvia; Surveillance and enforcement; Mollusc culture; Health and safety; Epidemiology.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1589.pdf
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Associations between farming practices and disease mortality of Pacific oyster Crassostrea gigas in a Mediterranean lagoon ArchiMer
Pernet, Fabrice; Lagarde, Franck; Le Gall, Patrik; Roque D'Orbcastel, Emmanuelle.
We present the first large-scale, high-resolution spatial and temporal pattern of disease mortality caused by Ostreid herpesvirus 1 (OsHV-1) that has affected Pacific oyster Crassostrea gigas in France every year since 2008, in relation to local farm use. Mortality was monitored in healthy oyster spat deployed at 106 locations in the Thau Mediterranean lagoon during the epizootic that occurred in spring 2011. We conducted a field survey of farming practices in the areas where the sentinel oysters were placed before the epizootic. We found that the risk of disease mortality in sentinel oysters was higher within the bivalve farming area than outside it, suggesting that the infection pressure is much higher in areas with intensive farming activity. The risk...
Tipo: Text Palavras-chave: Aquaculture; Epidemiology; OsHV-1; Shellfish farming.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00201/31248/30662.pdf
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Relance de l'huître plate. Rapport d'avancement des travaux 1988. ArchiMer
IFREMER (LA TRINITE/MER - LA TREMBLADE).
Un contrat de Plan État-Région Bretagne a été signé en 1983 pour financer des recherches sur la période 1984-1988 dans le but de permettre la relance de l'élevage de l'huître plate, à la suite de la deuxième épizootie due à un protozaire, Bonamia ostreae. Ce rapport présente les travaux réalisés et les résultats acquis durant l'année 1988, dans les domaines de la pathologie (étude de la maladie et des mécanismes de défense des mollusques) de la génétique (recherche de souches résistantes), de l'épidémiologie descriptive et de la zootechnie.
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Bonamia ostreae; Épidémiologie; Pathologie; Immunologie; Échantillonnage; Élevage; Shellfish; Ostrea edulis; Bonamia ostreae; Epidemiology; Pathology; Immunology; Sampling; Rearing.
Ano: 1989 URL: https://archimer.ifremer.fr/doc/00342/45284/44749.pdf
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Etudes pathologiques et epidemiologiques des lesions parasitaires chez Ostrea edulis : Premiers resultats d'un recherche prospective comparative chez les principales especes de mollusques des zones ostreicoles de Bretagne nord ArchiMer
Poder, M; Auffret, Mathieu; Balouet, G.
This paper is a contribution to the study of the Bonamia ostreae epizooty in french european oyster Ostrea edulis : parasite process (hemocytic parasitosis); experimental contaminations on parks and in tanks. First results of an epidemiological watch conducted in oyster culture sites show in molluks mytilidae, pectinidae, cardiidae, veneridae: the absence of Bonamia ostreae forms; the existence of different types of parasitism or commensalisus of inequal pathological importance and especially a parasite of the Marteilia genus in Tapes sp . digestive epithelium. Additionally, a new example of hemocytosercoma is observed in Cerastoderma edule .
Tipo: Text Palavras-chave: Ruditapes philippinarum; Tapes rhomboides; Tapes pullastra; Mytilus edulis; Cerastoderma edule; Ostrea edulis; Bivalvia; Marteilia; Bonamia ostreae; Comparative studies; Parasitic diseases; Commensalism; Parasitology; Marine mollusks; Epidemiology; Pathology; Pathologie; Mollusques; Bonamia ostreae; Epizootie; Ostrea edulis.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1182.pdf
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Revue systématique de la littérature relative aux facteurs de risque de mortalité des moules exploitées en France ArchiMer
Lupo, Coralie; Le Bouquin, Sophie.
La recherche bibliographique maximalisée réalisée dans le cadre de cette revue systématique de la littérature a permis de décrire le paysage de la connaissance existante sur les facteurs de risque de mortalité des espèces de moules M. edulis et M. galloprovincialis. Ces travaux ont abouti à la formalisation d’une méthodologie rigoureuse, transparente et reproductible pour la réalisation de revue systématique relative aux facteurs de risque de mortalité chez les coquillages marins. Cette revue a décrit la diversité des définitions de la mortalité des moules et ses facteurs de risque. Elle a également permis d’apprécier la quantité et la qualité des preuves recensées. Cette revue systématique a recensé 125 facteurs étudiés pour leur relation avec la...
Tipo: Text Palavras-chave: Mollusques marins; Coquillages bivalves; Moules; Mytilus edulis; Mytilus galloprovincialis; Mortalité; Facteurs de risque; Epidémiologie; Revue systématique; Bibliographie; Marine molluscs; Bivalve shellfish; Mussels; Mytilus edulis; Mytilus galloprovincialis; Mortality; Risk factors; Epidemiology; Systematic review; Bibliography.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59916/63099.pdf
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Prophylactic strategies and zootechnic measures, recent advances ArchiMer
Grizel, Henri.
The spread of major diseases of molluscs around the world and the increase of transfers need the development of adapted prophylactic strategies and the increase of research in epidemiology, immunology and genetics. The prophylactic strategies must be based on common laws with the establishment of a list of declarable pathogens, with basic sampling rules (sample size, frequency, definition of geographical areas) and with reglementation of the internal transfers. Moreover, specific adapted diagnosis should be developed to increase the sanitary control performance (e.g. ELISA test, DNA probe, cell culture). In the case of an infectious disease the epidemiology studies should be concerned, the relationship between the culture techniques and the disease, the...
Tipo: Text Palavras-chave: Bonamia ostreae; Mollusc culture; Aquaculture techniques; Pathology; Immunology; Genetics; Epidemiology; Fish diseases; Disease control.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1483.pdf
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Taxinomie et épidémiologie moléculaires du parasite Marteilia refringens Grizel et al. 1974 : intérêt pour la gestion du risque sanitaire en conchyliculture ArchiMer
Berthe, Franck.
Infectious diseases are currently recognised as a major limiting factor to development and sustainability of aquaculture of molluscs. The study is focused on the European flat oyster, Ostrea edulis and its paramyxean parasite Marteilia refringens. Results are contributing to the prevention of risk associated to infected stocks imports from endemic zones, by developing molecular diagnostic tools that are specific, sensible and reliable. Phylogeny and taxonomy of Marteilia refringens, previously impeded by microscopy limits, were clarified by sequencing the rDNA SSU, small sub-unit in the rRNA gene cluster. These new insights have practical implications discussed in a pragmatic polyphasic approach involving pathology and epidemiology. This approach led us to...
Tipo: Text Palavras-chave: Marteilia refringens; Marteilia maurini; Ostrea edulis; Mytilus edulis; Pathologie; Phylogénie moléculaire; Taxinomie; Epidémiologie; Marteilia refringens; Marteilia maurini; Ostrea edulis; Mytilus edulis; Molluscs diseases; Molecular phylogeny; Taxonomy; Epidemiology.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00109/21980/19594.pdf
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A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors ArchiMer
Nasfi, H.; Travers, Marie-agnes; De Lorgeril, Julien; Habib, C.; Sannie, T.; Sorieul, L.; Gerard, J.; Avarre, J. C.; Haffner, Philippe; Tourbiez, Delphine; Renault, Tristan; Furones, D.; Roque, A.; Pruzzo, C.; Cheslett, D.; Gdoura, R.; Vallaeys, T..
The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase...
Tipo: Text Palavras-chave: VNTR; Vibrio splendidus; Epidemiology; Vsm; OmpU; Crassotrea giga; Mortality.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00255/36666/35350.pdf
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Rearing practices identified as risk factors for ostreid herpesvirus 1 (OsHV-1) infection in Pacific oyster Crassostrea gigas spat ArchiMer
Normand, Julien; Blin, Jean-louis; Jouaux, Aude.
Early detection of Pacific oyster spat infected with ostreid herpesvirus 1 (OsHV-1) could prevent introduction of OsHV-1-infected individuals into farming areas or onshore rearing facilities, thus reducing the risk of infection of naïve oysters in such production systems. Experiments were conducted on several hundred oyster spat provided by producers in order to examine whether early rearing practices could be considered as potential risk factors for (1) OsHV-1 infection as detected by molecular methods and (2) spat mortality experimentally induced through thermal challenge. Spat groups collected on oyster beds and hatchery spat reared in growout areas during summer exhibited higher viral DNA contamination and mortalities during the trial than spat kept in...
Tipo: Text Palavras-chave: Oyster spat; Epidemiology; OsHV-1; QPCR; Thermal challenge.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00203/31407/29797.pdf
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Marteiliosis in molluscs: A review ArchiMer
Berthe, Franck; Leroux, Frederique; Adlard, Robert; Figueras, Antonio.
Among mollusc pathogens, paramyxeans are an important group of protistan parasites belonging to the genera Marteilia and Marteilioides. Martelia refringens and M. sydneyi are of particular concern given their potential impact on mollusc aquaculture world-wide. Aber disease and QX disease are currently listed by the OIE, the World Organisation for Animal Health. After more than thirty years of existence in the scientific literature, these organisms still pose questions and research challenges to investigators. This paper reviews current knowledge of the group and key references. The review was focused on taxonomy, epidemiology, pathology and potential control methods for these organisms in order to enhance understanding of paramyxean infection issues in...
Tipo: Text Palavras-chave: Paramyxean parasites; Diagnosis; Pathology; Epidemiology; Taxonomy.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-406.pdf
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Etudes épidémiologiques sur l'importance des coquillages en tant que facteur d'exposition de l'hépatite A - Approche méthodologique ArchiMer
Miossec, Laurence.
Si les coquillages sont considérés depuis longtemps comme des aliments à risque parce que consommés le plus souvent crus, les études épidémiologiques précises et complètes font défaut pour évaluer la part réelle des coquillages comme facteur de risque de certaines pathologies. Ce document décrit et discute les différentes approches méthodologiques d'une enquête épidémiologique dont le but est d'évaluer le risque d'hépatite A lié à la consommation de coquillages. En France métropolitaine, l'hépatite A est une maladie peu fréquente. Les données d'incidence sont évaluées entre 11 et 22 cas pour 100 000 habitants. Elles sont, cependant, imprécises. Parmi les facteurs de risque les plus fréquemment cités au niveau national, on trouve la contamination lors de...
Tipo: Text Palavras-chave: Hépatite A; Coquillages; Épidémiologie; Enquêtes cas-témoin; Enquêtes de cohorte; Épidémies. cas sporadiques.; Hepatitis A; Shellfish; Epidemiology; Case-control studies; Cohort studies; Outbreak; Sporadic cases..
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00105/21576/19156.pdf
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Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas ArchiMer
Petton, Bruno; De Lorgeril, Julien; Mitta, Guillaume; Daigle, Gaétan; Pernet, Fabrice; Alunno Bruscia, Marianne.
The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters (‘recipients’) in cohabitation in the laboratory with diseased oysters that were naturally infected in the field (‘donors’). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation...
Tipo: Text Palavras-chave: Aquaculture; Bivalve; Epidemiology; Health; Polymicrobial disease; OSHV-1; Pacific oyster mortality syndrome; POMS.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00507/61870/65940.pdf
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Relance de l'huître plate. Rapport de synthèse des travaux 1990 ArchiMer
Martin, Anne-genevieve.
Une convention a été signée en 1990 avec la Région Bretagne pour financer la poursuite des recherches dans le but d'accélérer la relance de l'élevage de l'huître plate, à la suite de la deuxième épizootie due à un protozoaire, Bonamia ostreae. Ce rapport présente les travaux réalisés et les résultats acquis durant l'année 1990, dans les domaines de l'épidémiologie descriptive, de la zootechnie, de la génétique (recherche d'espèces ou de souches résistantes) et de l'immuno-pathologie (étude de la maladie et des mécanismes de défense des mollusques). Durant l'année 1990, la situation épidémiologique des élevages d'huîtres plates en eau profonde a évolué différemment selon les secteurs: relativement stable en Baie du Mont st Michel, elle présente de brusques...
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Bonamia ostreae; Épidémiologie; Pathologie; Immunologie; Génétique; Échantillonnage; Élevage; Shellfish; Ostrea edulis; Bonamia ostreae; Epidemiology; Pathology; Immunology; Genetic; Sampling; Rearing.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00102/21320/18942.pdf
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Establishing causal link between an infectious agent and mortalities in marine molluscan aquaculture on the example of Bonamia ostreae and Herpèsvirosis in oysters : proposal of a causal grid analysis ArchiMer
Thebault, Anne; Cochennec, Nathalie; Arzul, Isabelle; Renault, Tristan.
In marine molluscan aqauculture, infectious agents are regularly cited in literature associated with mass mortalities as emergin diseases, but the causal link is often based on very few argumeents. 24 ways to demonstrate causal links from epidemiolgical, ecological and marine pathology literature feasible for marine molluscs are given. Those criteria were applied to the ewample of Bonamia ostreae for Ostrea edulis and Herpesvirus for spats and larvae of Crassostrea gigas. Three principles could be applied in marine field for choosing criteria wich should be taken in priority : the demonstration must be given at three levels of organization, organ individual, population, the demonstration must keeps maximum epidemiological criterias, and then mixes...
Tipo: Text Palavras-chave: Herpesvirus; Bonamia ostrea; Crassostrea gigas; Ostrea edulis; Marine pathology; Ecology; Epidemiology; Infectious agents; Molluscan aquaculture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3341.pdf
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Factors influencing disease-induced mortality of Pacific oysters Crassostrea gigas ArchiMer
Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice.
Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from <14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented...
Tipo: Text Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf
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