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Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313 52
Palit,Soumen; Banerjee,Rintu.
The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus circulans; Solid state fermentation; Alpha- amylase; Extraction.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015
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Neural modeling of bromelain extraction by reversed micelles 52
Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Tambourgi,Elias Basile.
A pulsed-cap microcolumn was used for bromelain extraction from pineapple juice by reversed micelles. The cationic micellar solution used BDBAC as the surfactant, isooctane as the solvent and hexanol as the co-solvent. In order to capture the dynamic behavior and the nonlinearities of the column, the operating conditions were modified in accordance with the central composite design for the experiment, using the ratio between the light phase flow rate and the total flow rate, and the time interval between pulses. The effects on the purification factor and on total protein yield were modeled via neural networks. The best topology was defined as 16-9-2, and the input layer was a moving window of the independent variables. The neural model successfully...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Reversed micelles; Extraction; Neural networks; Pineapple.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200026
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Extraction of red cabbage anthocyanins: optimization of the operation conditions of the column process 52
Xavier,Marcelo Fonseca; Lopes,Toni Jefferson; Quadri,Mara Gabriela Novy; Quadri,Marintho Bastos.
The aim of this work was to extract anthocyanins from the red cabbage. Batch studies under several extraction conditions indicated that acetic acid in aqueous solution (10% V/V) was the best solvent, used in the proportion of 0.25 g of red cabbage mL-1. At this condition, column assays were carried out to evaluate the influence of the ionic force, pH, solvent flow rate, recirculated volume of red cabbage juice and the mass of red cabbage. Results showed that the pH, recirculation and mass of red cabbage had statistically significant effects, where the optimum operation conditions found for the process were pH 2.3, recirculation volume of the solvent 0.83 L and mass of red cabbage 50 g.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanins; Extraction; Red cabbage; Experimental design; Optimization.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100018
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Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar 52
Montañez Sáenz,Julio; Téllez,Alejandro; de la Garza,Heliodoro; de la Luz Reyes,María; Contreras-Esquivel,Juan Carlos; Aguilar,Cristóbal Noé.
Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extraction; Purification; Properties; Pectinesterase; Potato.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007
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Extraction time for soybean isoflavone determination 52
Carrão-Panizzi,Mercedes C.; Favoni,Silvana Pedroso de Goés; Kikuchi,Akio.
Studies were carried out on the optimization of the extraction procedures for isoflavones determination in soybean by HPLC. Isoflavones were extracted from 100 mg samples of full fat soybean flour "Kinako" (milled toasted grains). Recovery of average total isoflavones was higher when extraction was performed with agitation(217.2 mg/100g), than without agitation(191.0 mg/100g). Isoflavone extraction without agitation for 1,4, 20 and 24 hours, were equally efficient for total and individual compounds. These results suggested that an efficient isoflavone recovery could be achieved with extraction for one hour with agitation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybeans; Isoflavones; Glucosides; Aglucones; Extraction; Ethanol; HPLC.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600015
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Characterization of Seed Oils of Pterodon emarginatus Vogel (Sucupira) Obtained by Different Extraction Methods 52
Costa-Singh,Tainara; Jorge,Neuza.
Abstract The present study aims to evaluate the influence of extraction methods on the quality of bioactive compounds and antioxidant capacity in Pterodon emarginatusVogel (sucupira) oils. The oils were extracted from the sucupira seeds by Soxhlet and by the extraction system of Bligh & Dyer. The oils were analyzed as to fatty acid profile, tocopherols, phytosterols, carotenoids, total phenolics, and total antioxidant capacity. The extraction by Soxhlet showed better yield of total lipids and was more efficient to extract tocopherols, phytosterols, and carotenoids, besides presenting better antioxidant activity by DPPH•. However, this method showed insufficient capacity to extract the polar lipid components of the sample, as evidenced by the low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Bioactives; Extraction; Oxidative stability; Chromatography.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100506
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Extraction model of low methoxyl pectin from apple pomace effects of acid concentration and time on the process and the product 52
Fertonani,Heloísa Cristina Ramos; Scabio,Ardalla; Carneiro,Eliana Beleski Borba; Schemim,Maria Helene Canteri; Nogueira,Alessandro; Wosiacki,Gilvan.
In Brazil, one of the top apple producing countries in the world, apple processing is an increasing activity, with pomace as the main by-product. To extract pectin from pomace, factors affecting process and product should be studied for optimization. A model to produce LMP directly from dried apple pomace was established observing the effects of HNO3 concentration and the time of reaction at 97ºC, analyzed from a statistical and practical point of view. The model for gravimetric yield (R² =0.9834) predicts the highest value of 20.07 g/100 g (126 mM; 14.07 min) of a pectin with a degree of esterification of 48.49%. The model for degree of esterification of extracted pectin (R²= 0.9797) predicts the lowest value of 43.73% (200 mM; 10.07 min) with a yield of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple; Pectin; Extraction; LMP; Nitric acid.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100023
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Development and optimization of extractives from Astronium urundeuva (allemão) Engl. by factorial design 52
Cunha,Fabíola Pessoa da; Costa,Luana Janine Lopes da; Fernandes,Ana Josane Dantas; Souza,Tatiane Pereira de; Soares,Luiz Alberto Lira.
The purpose of this study was to evaluate the influences from variables of preparation on dry residue and total tannins from macerates of Astronium urundeuva barks. Thus, a 3 ² factorial design was used to study the importance of plant proportion (10, 15 and 20 %, w/v) and the ethanol concentration (40, 60 and 80 %, v/v) on the selected response. Mathematical models were fitted according to experimental data. The validated equations were used to generate response surfaces. The analysis of the surfaces showed that the optimum conditions to obtain extractive preparations with high extractive efficiency for tannins were: 10 % (w/v) of plant with ethanol 40 % (v/v) as solvent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astronium urundeuva; Extraction; Optimization; Factorial Design.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300017
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Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 58
Sun,Xinyi; Hao,Long; Ma,Hua; Li,Tong; Zheng,Lan; Ma,Zhao; Zhai,Guoyin; Wang,Liqin; Gao,Shanglong; Liu,Xiaonan; Jia,Mengshi; Jia,Le.
The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pleurotus eryngii SI-02; Exopolysaccharide; Extraction; Antioxidant activity; In vitro.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009
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Assessment of four different detergents used to extract membrane proteins from Xylella fastidiosa by two-dimensional electrophoresis 58
Di Ciero,Luciana; Bellato,Cláudia de M.; Meinhardt,Lyndel W.; Ferrari,Fernanda; Castellari,Rafael R.; Marangoni,Sérgio; Novello,José C..
Four different detergents, ASB 14, SB 3-10, CHAPS and Triton X100, were utilized to determine the optimal detergent for the solubilization of membrane proteins from the phytopathogenic bacterium Xylella fastidiosa. These proteins were differentially solubilized in distinct buffers containing the detergent and subjected to bidimensional electrophoresis within the non-linear pH range of 3-10. The detergents ASB 14 and SB 3-10 were the most effective revealing 221 and 157 spots, respectively. CHAPS and Triton X100 were less effective and revealed only 72 and 43 spots, respectively. MALDI-TOF tryptic peptide mass fingerprinting of 18 excised proteins from the ASB 14 treatment revealed that 83% were membrane proteins and that the theoretical efficiency of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: X. fastidiosa; 2-DE; Extraction; Proteome; Solubilization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200018
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Extraction of Soluble Polysaccharides from Grape Skins 118
Gil Cortiella,Mariona; Peña-Neira,Álvaro.
Obtaining polysaccharides from grape cell walls is an interesting topic for the wine industry given its implications with regards to wine treatments and potential sensory attributes. In the present work, several conditions (i.e., a chelating agent, pH, titratable acidity, extraction time, number of successive extractions, solid to liquid ratio and alcoholic fermentation) were tested to assess their effects on the yield of soluble polysaccharides obtained from grape skins. Among the four tested solvents (i.e., water, tartaric acid, ammonium oxalate, and ethylenediaminetetraacetic acid), aqueous ammonium oxalate was the most effective for polysaccharide extraction but also produced the highest amount of protein impurities. Increasing the number of successive...
Tipo: Journal article Palavras-chave: Carménère; Extraction; Grape skin polysaccharides; Pectin; Polysaccharides; Wine.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000100009
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Ultrasound pretreatment as an alternative to improve essential oils extraction 65
Nora,Flávia Michelon Dalla; Borges,Caroline Dellinghausen.
ABSTRACT: Essential oils are substances originated from plants in general. These compounds are well known to have a high biological activity, specially the antioxidant and antimicrobial. Several extraction techniques are employed to obtain these substances. However, the majority of these techniques require a long extraction time. In this sense, innovative and alternative extraction techniques, such as ultrasound, have recently been the target of studies. In view of the small amount of publications using ultrasonic pretreatment, this review aimed to congregate current relevant information on ultrasound-assisted extraction of essential oils. In this sense, theoretical aspects, such as the main factors that influence the performance of this technique as well...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Food; Extraction; Ultrasound; Green chemistry.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000900753
Registros recuperados: 52
Primeira ... 123 ... Última
 

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