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| Registros recuperados: 425 | |
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| ANDRIOLI, A.; GOUVEIA, A. M. G.; PINHEIRO, R. R.. |
| A partir de 1976, importações de caprinos de raças exóticas, procedentes de vários países, buscaram a introdução de potencial genético leiteiro, tendo ocorrido também a efetiva introdução de agentes infecciosos no Brasil pela importação de caprinos sem adequados critérios sanitários, com consequente dispersão de doenças infecciosas entre os rebanhos nacionais, dentre elas a CAE - artrite encefalite caprina. Os avanços das técnicas de conservação de sêmen, aliados ao aumento da importância econ0mica da caprinocultura favorecem o seu intercâmbio nacional e internacional. Somando-se a isto, a inseminação artificial possibilita a concepção de um expressivo número de fêmeas, num curta intervalo de tempo, no entanto, pouco se sabe do risco da transição de... |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: CAEV; Artrite Encefalite Caprina; Caprine arthritis encephalit virus; Semen diluents.; PCR; Caprino; Reprodução animal; Sêmen; Seleção; Transmissão de doença; Goats; Reproduction; Disease transmission; Infection.. |
| Ano: 2003 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/531309 |
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| SANTOS, C. A. F.; SÁ, J. F. de; GAMA, R. N. C. de S.; OLIVEIRA, A. E. S. de; OLIVEIRA, V. R.. |
| A identificação de diferentes tipos de citoplasmas associados à macho-esterilidade em cebola foi facilitada com a aplicação de marcadores de DNA em reações de reação em cadeia da polimerase (PCR), auxiliando no desenvolvimento de híbridos. O objetivo deste trabalho foi identificar, por PCR, o tipo de citoplasma presente em acessos brasileiro de cebola, de forma a facilitar o desenvolvimento de híbridos. O DNA de cinco plantas de cada acesso foi extraído de acordo com o protocolo do CTAB 2x. Entre os 30 acessos analisados, observou-se a presença dos três tipos de citoplasma. Doze acessos apresentaram tipo de citoplasma exclusivamente ?N?, sete exclusivamente ?S? e apenas um exclusivamente ?T?. Seis acessos apresentaram mistura entre os tipos de citoplasma... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: CMS; PCR; Macho-esterilidade; Melhoramento genético; Onion; Cebola; Recurso genético; Melhoramento vegetal; Allium Cepa; Citoplasma; Hibridação; Marcador Molecular. |
| Ano: 2016 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1064469 |
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| Pepin, Jean-francois; Riou, Antoine; Renault, Tristan. |
| Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf |
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| Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan. |
| Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted... |
| Tipo: Text |
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf |
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| Balabaud, Karen. |
| Avec 30 000 tonnes d'huîtres produites chaque année, l'ostréiculture est l'activité principale du bassin de Marennes-Oléron. Caractérisé par une très forte interaction avec les milieux professionnels de la conchyliculture, l'Ifremer se doit avant tout de répondre à leurs questions, en particulier en matière de croissance, de reproduction et surtout en matière de mortalité de l'huître creuse. En effet, l'huître portugaise Crassostrea angulata fut décimée de 1968 à 1972 par un agent pathogène de type iridovirus. Pour faire face à cette disparition des populations d'huîtres japonaises Crassostrea gigas sont alors introduites en France. Mais, les professionnels s'interrogent sur la bonne adaptation de l'huître creuse japonaise à l'environnement français et... |
| Tipo: Text |
Palavras-chave: Génétique; Croisement; Isolement reproductif; Huîtres creuses; Hybrides; Marqueurs moléculaires; PCR; Crassostrea gigas; Crassostrea angulata. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00033/14441/11740.pdf |
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| Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal. |
| Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate. |
| Tipo: Text |
Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf |
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| Michel, Justine. |
| En 2004 et 2005, une étude menée par le REPAMO (Réseau de Pathologie des Mollusques) a montré que des parasites du genre Perkinsus étaient présentas au sein des principaux domaines français de production de palourdes: La Mer Méditerranée, l'Océan Atlantique et la Manche avec une forte prévalence amis aucun cas de mortalités associées. Cependant, ces résultats doivent être complétés par des analyses moléculaires plus spécifiques de façon à déterminer la ou les espèces de Perkinsus détectés. D'autre, part, l'étude a montré que différents facteurs environnementaux (température, salinité...) liés à la répartition géographique influencent le développement de parasite. Ces résultats suggèrent l'existence d'une variabilité génétique chez le ou les parasites du... |
| Tipo: Text |
Palavras-chave: Parasite; Perkinsus; PCR; Ruditapes decussatus; Ruditapes philippinarum; Variabilité génétique. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00033/14453/11754.pdf |
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| Gawler, A; Beecher, J; Brandao, J; Carroll, N; Falcao, L; Gourmelon, Michele; Masterson, B; Nunes, B; Porter, J; Rince, A; Rodrigues, R; Thorp, M; Walters, J; Meijer, W. |
| The recent implementation of the Revised Bathing Water Directive in the European Union has highlighted the need for development of effective methods to differentiate between sources of faecal contamination. It had previously been shown that amplification of 16S rRNA genes of host-specific Bacteriodales species using the HF183F and CF128F primers could be used as markers for human and bovine faecal contamination in the United States. This paper determined the sensitivity and specificity of these markers in four Atlantic Rim countries (France, Ireland, Portugal and the United Kingdom) to evaluate their usefulness in determining the origin of faecal contamination. It was shown that the HF183F marker displayed high sensitivity (80-100%) and specificity... |
| Tipo: Text |
Palavras-chave: Sensitivity; Specificity; PCR; Microbial source tracking; Bacteriodales. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4957.pdf |
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| Killelea, Tom; Ralec, Celine; Bosse, Audrey; Henneke, Ghislaine. |
| DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing, and nucleic acid based diagnostics. Taking into account the multiple DNA amplification techniques in use, different DNA polymerases must be optimized for each type of application. One of the current tendencies is to reengineer or to discover new DNA polymerases with increased performance and broadened substrate spectra. At present, there is a great demand for such enzymes in applications, e.g., forensics or paleogenomics. Current major limitations hinge on the inability of conventional PCR enzymes, such as Taq, to amplify degraded or low amounts of template DNA. Besides,... |
| Tipo: Text |
Palavras-chave: DNA polymerase; Archaea; Family D; PCR; Pyrococcus. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00193/30450/28871.pdf |
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| Faure, Stephanie. |
| La marteiliose est une maladie causée par Marteilia refringens chez l'huître plate, Ostrea edulis. Ce parasite induit des mortalités importantes chez ces mollusques bivalves. Une autre espèce, Marteilia maurini, parasite la moule, Mytilus edulis, sans engendrer de mortalités notables. Des cas de coinfections des moules par ces 2 parasites ont été observés. La moule pourrait donc être vecteur de Marteilia refringens. Des études sur la taxinomie de Marteilia refringens ont démontré qu'il existe un polymorphisme entre les 2 espèces dans l'ITS1. De ce fait, une technique de PCR-RFLP a été mise au point pour discriminer les 2 espèces. Le but de cette étude est d'éclaircir le statut de la moule dans le cycle biologique de Marteilia refringens par deux approches... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Marteilia maurini; Mytilus edulis; Vecteur; PCR-RFLP; PCR; Pathologie. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00032/14343/11627.pdf |
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| Vigneron, Vassilia. |
| Depuis 1991, des mortalités massives associées à des infections à herpès virus sont rapportées chez des larves et des juvéniles de différentes espèces de bivalves dans différentes régions du globe. Les études ont porté essentiellement sur la mise en évidence de la présence de ce type de virus chez les coquillages d'intérêt économique. Actuellement, l'intérêt se porte sur la persistance du virus dans le milieu marin, et cela dans le but de déterminer si le milieu constitue ou non un moyen de contamination des animaux. Le travail a constitué en une étude sur la persistance de la détection de l'ADN d'herpès virus (OsHV-1) afin de mieux appréhender les modes de dissémination et de transmission du virus. Dans un premier temps, la stabilité de l'ADN purifié et... |
| Tipo: Text |
Palavras-chave: Herpèsvirus; Eau de mer; Détection; PCR; Persistance; Stabilité. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00032/14350/11634.pdf |
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| Dundon, William G.; Arzul, Isabelle; Omnes, Emmanuelle; Robert, Maeva; Magnabosco, Cristian; Zambon, Michela; Gennari, Lorenzo; Toffan, Anna; Terregino, Calogero; Capua, Ilaria; Arcangeli, Giuseppe. |
| In April 2010, the sampling of juvenile Pacific cupped oysters originating from France was undertaken from a farm located off the coast of the Marche region in Italy. Samples were sent to the national reference laboratory for mollusc diseases for bacteriological, histological, electron microscopical and molecular analysis. Classical and Real-Time PCR indicated the presence of the microvariant (OsHV-1 mu var) of Type 1 Ostreid Herpes virus (OsHV-1) despite the absence of clinical and pathological signs normally associated with the presence of this variant. Further molecular and sequencing analysis revealed the presence of OsHV-1 par and OsHV-1 DNA in one oyster indicating that simultaneous detection of both viruses is possible. This is the first report of... |
| Tipo: Text |
Palavras-chave: Pacific cupped oyster; Ostreid Herpes virus 1 mu var; PCR; Italy. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00035/14585/11968.pdf |
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| Gagne, N; Cochennec, Nathalie; Stephenson, M; Mcgladdery, S; Meyer, G; Bower, S. |
| As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist... |
| Tipo: Text |
Palavras-chave: Histology; PCR; In situ hybridization; Ostrea edulis; Mikrocytos. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4475.pdf |
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| Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. |
| Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... |
| Tipo: Text |
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf |
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| Registros recuperados: 425 | |
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