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Registros recuperados: 154 | |
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Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan. |
The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed... |
Tipo: Text |
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf |
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Maggi, Pierre. |
The effect of the 2 pesticides most frequently used against mosquitoes was tested on a number of marine organisms. Growth inhibition was tested using Phaeodactylum tricornutum and Gyrosigma spencer (algae). To 0.5 ml of culture inoculum containing similar to 100000 cells in exponential growth phase, 9.5 ml of insecticide in varying concentrations were added and the growth was recorded every 48 hour; LG sub(50) was determined. Muscular activity and filtration rate of Crassostrea angulata were tested. The acute toxicity was tested using 4 species of gastropods, 2 lamellibranchs, 5 crustaceans, 1 coelenterate and 3 fishes; the species used were: Gibbula umbilicalis, Purpura lapillus, Littorina littorea, Patella vulgata, Mytilus edulis, Cardium edule, Artemia... |
Tipo: Text |
Palavras-chave: Marine organisms; Toxicity test; Fenitrothion; Abate; Pesticides. |
Ano: 1973 |
URL: http://archimer.ifremer.fr/doc/1973/publication-2229.pdf |
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Pavoni, B; Caliceti, M; Sperni, L; Sfriso, A. |
Samples of seven genera of seaweeds (Ulva, Gracilaria, Porphyra, Grateloupia, Undaria, Fucus and Cystoseira), which can be used as environmental biomonitors and in several applications like human alimentation, animal feeding and cosmetics, were collected in four sampling sites in the Lagoon of Venice in spring and autumn 1999 with the aim of determining the contamination due to organic micropollutants (polychlorinated biphenyls. PCBs; chlorinated pesticides; polycyclic aromatic hydrocarbons, PAHs). On an average, most of the considered species were contaminated especially by PAHs (up to 56 ng g(-1) dry wt. (dw)) whereas concentrations of PCBs and pesticides in macroalgal tissues were significantly correlated and did not exceed 5 ng g-1 dw. Among the... |
Tipo: Text |
Palavras-chave: Macro-algues; Lagune de Venise; HAP; PCB; Pesticides; Seaweeds; Venice Lagoon; PAHs; PCBs; Pesticides. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00321/43250/42981.pdf |
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Sharma, A; Battersby, N; Stewart, D. |
The Minimal Inhibitory Concentration (MIC) of a biocide is usually determined by adding it in various concentrations to a suitable growth medium which is then inoculated with the test bacterium. These cultures are then observed for up to 28 days for growth of the organism as shown by its production of black ferrous sulphide. A method has been developed whereby the MIC of a biocide can be found within 4 days. Instead of noting ferrous sulphide production, this new method measures changes in: 1) optical density and 2) sulphate reduction of a logarithmic culture of the test strain exposed to the biocide. |
Tipo: Text |
Palavras-chave: Bacteria; Microorganisms; Pesticides; Analytical techniques; Sulfate reduction. |
Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-1016.pdf |
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Stachowski-haberkorn, Sabine; Guesdon, Stephane; Bechemin, Christian; Chiffoleau, Jean-francois; Brach-papa, Christophe; Soudant, Philippe; Beker, Beatriz; Jadas-hécart, Alain. |
TOPHYPAC is a field study that was conducted in the Charente river estuary (Marennes-Oléron bay). The main goal was to assess the possible impact of chemical contamination with copper and pesticides on phytoplankton communities. Physical, chemical (nutrients, metals and pesticides) and biological (phytoplankton community) parameters were monitored in the field during three years, from 2011 to 2014. In 2012 and 2013, field sampling was coupled with experimental exposure of phytoplankton communities to copper and to an herbicide cocktail, after transportation to the lab: the effects were assessed using photosynthesis endpoint and cellular densities. TOPHYPAC allowed to set the framework of Charente estuary contamination with pesticides: herbicides glyphosate... |
Tipo: Text |
Palavras-chave: Continuum eau douce/eau côtière; Pesticides; Métaux; Communautés phytoplanctoniques; Efficacité de photosynthèse; PICT; Tolérance; Cytométrie en flux; Toxicité chronique; Freshwater/coastal water continuum; Pesticides; Metals; Phytoplankton communities; Photosynthesis efficiency; PICT; Tolerance; Flow cytometry; Chronic toxicity. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00300/41115/40289.pdf |
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Coat, Sophie; Bocquene, Gilles; Godard, Eric. |
Martinique is a French overseas department whose economy relies heavily on agriculture. Organochlorine pesticides, mainly chlordecone, were used for banana cultivation to eradicate banana weevil over a period of 40 years. Chlordecone is chemically stable,and has a strong affinity for fatty tissues. It is therefore able to bioaccumulate in animals and thereby represent a threat to ecosystems and man. Soils from banana plantations in Martinique are heavily contaminated with chlordecone. Possible transfer of these molecules from agricultural watersheds to the aquatic environment and the organisms that live in it is feared. The hypothesis that ecosystems of Martinique might be highly contaminated with this organochlorine pesticide was investigated. Chlordecone... |
Tipo: Text |
Palavras-chave: W Atlantic; Caribbean Islands; Food web; Aquatic organisms; Chlordecone; Contamination; Pesticides. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1662.pdf |
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Moreau, Kevin. |
Des mortalités chez l'huître creuse, Crassostrea gigas, ont été associées au virus OsHV-1 (Oyster herpes virus 1) au stade larvaire et chez des juvéniles. Cependant, aucune mortalité liée à ce virus n'a été rapportée chez les animaux adultes. Cette observation laisse suspecter l'existence de mécanismes dirigés contre ce virus chez cette espèce. Il a peu de connaissances à l'heure actuelle sur les mécanismes de défense antivirale chez les bivalves, en particulier chez l'huître creuse, C. gigas, comme étant sur-exprimés en présence du virus. Ces gènes, s'ils ne peuvent être considérés comme des gènes de défense à proprement parlé, participent à la réponse de l'huître vis à vis du virus. Par ailleurs, des polluants comme les pesticides sont susceptibles de... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; OsHV-1; Pesticides; Expression génétique; RT-PCR semi-quantative. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00032/14339/11622.pdf |
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Dupraz, Valentin; Stachowski-haberkorn, Sabine; Wicquart, Jérémy; Tapie, Nathalie; Budzinski, Hélène; Akcha, Farida. |
Pesticides used in viticulture create a potential risk for the aquatic environment due to drift during application, runoff and soil leaching. The toxicity of sixteen pesticides and one metabolite were evaluated on the growth of two marine microalgae, Tisochrysis lutea and Skeletonema marinoi, in 96-h exposure assays conducted in microplates. For each substance, concentrations of stock solutions were analytically measured and abiotic assays were performed to evaluate the chemical stability of pesticides in microplates. For two chemicals, microalgae exposures were run simultaneously in microplates and culture flasks to compare EC50 calculated from the two exposure systems. Results from chemical analyses demonstrated the low stability of hydrophobic... |
Tipo: Text |
Palavras-chave: Microalgae; Microplates; Screening; Pesticides; Toxicity; Exposure characterisation. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00474/58558/61113.pdf |
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Akcha, Farida; Arzul, Genevieve; Rousseau, Sabrina; Bardouil, Michele. |
The alkaline comet assay was tested on different microalgae: the clinofiagellates, Karenia mikimotoi and Alexandrium minuturn, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1 h, 5 and 100 pM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 pM with a concentration-dependent effect. C. gracilis was too small in size (4 mu m) to be correctly analysed. For A. minuturn, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of... |
Tipo: Text |
Palavras-chave: Comet assay; Genotoxicity; Pesticides; Phytoplankton. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4504.pdf |
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Bocquene, Gilles; Franco, Alain. |
In January and February 2002, the presence of certain agricultural pesticides throughout the coastline of the Caribbean island of Martinique was investigated. The tropical climate of the French West Indies is suitable for banana production, which requires intensive use of pesticides. An inventory of all pesticides used on the island (compounds and tonnage) was compiled. Surveys and analyses revealed the presence of pesticides in the plumes of seven rivers. The organochlorine chlordecone and metabolites of aldicarb were detected at nearly all of the monitored sites, even though the use of chlordecone has been prohibited since 1993. Two triazines (ametryn and simazine) were also identified. The concentrations of carbamates and triazines detected in the water... |
Tipo: Text |
Palavras-chave: Aldicarb; Chlordecone; Triazines; Pesticides; Contamination; Martinique Island. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-879.pdf |
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Di Poi, Carole; Costil, Katherine; Bouchart, Valerie; Halm-lemeille, Marie-pierre. |
Despite a growing scientific attention on ecological impact of emerging pollutants (EPs) such as pharmaceuticals, personal care products, and pesticides, knowledge gaps remain regarding mixture toxicity and effects on aquatic organisms. Several EPs were screened in seawater (Normandy, France), and the ecotoxicity of five compounds, chosen on their occurrence in ecosystems and use worldwide, was assessed and were the biocides methylparaben (MP) and triclosan (TCS), a pesticide degradation product (AMPA), and the pharmaceuticals venlafaxine (VEN) and carbamazepine (CBZ). The acute or sub-chronic toxicity, alone or in binary/ternary mixtures of three of them (CBZ, AMPA, and MP), was assessed on one marine and two freshwater organisms: Crassostrea gigas,... |
Tipo: Text |
Palavras-chave: Pharmaceuticals; Personal care products; Pesticides; Mixture and single-compound toxicity; Freshwater species; Marine bivalve. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00389/50004/52260.pdf |
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Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan. |
To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR).... |
Tipo: Text |
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf |
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Registros recuperados: 154 | |
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