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Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 Agronomy
Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran.
In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM...
Tipo: Laboratory analysis Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279
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Substratos e extrato pirolenhoso no cultivo de orquídeas brasileiras Cattleya intermédia (John Lindley) e Miltonia clowesii (John Lindley) (Orchidaceae) - DOI: 10.4025/actasciagron.v32i1.714 Agronomy
Schnitzer, Jenniffer Aparecida; UEL; Faria, Ricardo Tadeu de; UEL; Ventura, Mauricio Ursi; UEL; Sorace, Mauren; UEL.
The vegetative and root development of the species of Brazilian orchids Cattleya intermedia (John Lindley) and Miltonia clowesii (John Lindley) using different substrates and pyroligneous extract were assessed. Seedlings from in vitro propagation were transferred to vessels with: T1 - Pinus bark (Pi), coconut fibers (Co) and charred peel of rice (Cr); T2 – PiCoCr and vegetal charcoal (Ch), T3 – PiCoCrCh with the charcoal treated with pyroligneous extract (PE); T4 - CoCrChEP and T5 - CoCrCh. The materials used in substrates were placed in the same ratio. Plant height, number of roots, length of the greater root, number of buddings, total fresh mass and pH were assessed seven months after experiment onset. For Para C. intermedia, T3 was superior than the...
Tipo: Pesquisa Empírica de Campo Palavras-chave: 5.01.00.00-9 Agronomia Orchidaceae; Vinagre de madeira; Carvão; Ácido pirolenhoso; Desenvolvimento vegetativo; Enraizamento 5.01.03.00-8 Orchidaceae; Wood vinegar; Charcoal; Pyroligneous acid; Vegetative development; Rooting.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/714
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Morphophysiology, Phenotypic and Molecular Diversity of Auxin-induced Passiflora mucronata Lam. (Passifloraceae) Anais da ABC (AABC)
FRANÇA,JULIANY M.; VENIAL,LUCIMARA R.; COSTA,ELOÁ B.; SCHMILDT,EDILSON R.; SCHMILDT,OMAR; BERNARDES,PAULA M.; TATAGIBA,SANDRO D.; LOPES,JOSÉ C.; FERREIRA,MARCIA F.S.; ALEXANDRE,RODRIGO S..
ABSTRACT Genetic diversity allows identification of potential intraspecific genotypes in the genus Passiflora. The objective of this study was to examine the morphological and genetic diversity of auxin-induced Passiflora mucronata. The experiments were arranged in a complete randomized block design, with a 9 x 2 factorial arrangement (nine genotypes x presence and absence of auxin, indole-3-butyric acid (IBA)), with four replicates of 16 cuttings. The rooting and vegetative growth responses were variable. Genotype 5 was more responsive in the absence of IBA and genotypes 3, 8 and 9 were more responsive in the presence of IBA. Auxin increased rooting rate and percentage, reducing the average time of root protrusion in eight days. IBA also contributed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indol-3-butyric acid; Rooting; Genotypes; Restinga passion fruit; Molecular markers ISSR; Propagation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401799
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Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina Biocell
Caro,Luis A.; Santecchia,Natalia; Marinangeli,Pablo A.; Curvetto,Néstor R.; Hernández,Luis F..
The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium rhizogenes; Algarrobo; Hairy roots; Indole-3-butyric acid; Nothofagus nervosa; Prosopis chilensis; Raulí; Rooting.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300002
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Plant regeneration after long term callus culture in clones of Asparagus officinalis L. Biocell
Pontaroli,A. C.; Camadro,E. L..
Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l-1 2,4-D and 1 mg.l-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone ‘265' on MS medium with 5 mg.l-1 2iP, 1 mg.l-1 IAA, 3% sucrose and 0.9% agar. The highest...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Callus; Shoot primordia induction; Rooting; Medium x genotype interactions.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300009
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Effects of plant growth promoting rhizobacteria (PGPR) on rooting and root growth of kiwifruit (Actinidia deliciosa) stem cuttings Biol. Res.
ERTURK,YASAR; ERCISLI,SEZAI; HAZNEDAR,AYHAN; CAKMAKCI,RAMAZAN.
The effects of plant growth promoting rhizobacteria (PGPR) on the rooting and root growth of semi-hardwood and hardwood kiwifruit stem cuttings were investigated. The PGPR used were Bacillus RC23, Paenibacillus polymyxa RC05, Bacillus subtilis OSU142, Bacillus RC03, Comamonas acidovorans RC41, Bacillus megaterium RC01 and Bacillus simplex RC19. All the bacteria showed indole-3-acetic acid (IAA) producing capacity. Among the PGPR used, the highest rooting ratios were obtained at 47.50% for semi-hardwood stem cuttings from Bacillus RC03 and Bacillus simplex RC19 treatments and 42.50% for hardwood stem cuttings from Bacillus RC03. As well, Comamonas acidovorans RC41 inoculations indicated higher value than control treatments. The results suggest that these...
Tipo: Journal article Palavras-chave: PGPR; Stem cuttings; Kiwifruit; Rooting; Auxin.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100011
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Micropropagation of Alibertia edulis Rich BABT
Silva,Fernanda Augusta Boato da; Pereira,Luiz Alfredo Rodrigues; Silveira,Conceição Eneida dos Santos.
The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA) alone or combined with indole-3-butyric acid (IBA) was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Alibertia edulis; Rooting; Germination; Activated charcoal.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600004
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Studies on stem cuttings of kiwi (Actinidia chinensis PL. CV Bruno) BABT
Ono,Elizabeth O.; Rodrigues,João D.; Pinho,Sheila Z. de.
The work was carried out to study the effects of some auxins and boron in the rooting of kiwi (Actinidia chinensis Planch cv Bruno) stem cuttings.. These cuttings were treated on the base individually with H2O; NAA 300 mg.L-1; IBA 300 mg.L-1; NAA 300 mg.L-1 + Boron; IBA 300 mg.L-1 + Boron; NAA 0,5%-talc and IBA 0,5%-talc. After the treatments, the cuttings were placed in styrofoam trays with vermiculite under moist conditions for 120 days. The evaluation of auxin and boric acid effects were made by observing rooted stem cuttings percentage; reducing and total sugar analysis (g/100 g of dry matter); and tryptophan analysis (in µg/100 mg of dry matter). The effects of such treatments were observed during four seasons of the year. The results showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinidia chinensis; NAA; IBA; Boron; Rooting.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100007
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Establishment of an Embryonic Tip Regeneration System of Soybean BABT
Si-yan,LIU; Guang-long,LI; Jing,QU; Shu-yan,GUAN; Dan,YAO; Pi-wu,WANG; Si-chen,LIU; Yang,JIA; Peng-fei,GUAN; Long-qing,WANG.
ABSTRACT In order to establish an optimal soybean embryonic tip regeneration system that can serve as soybean genetic transformation receptor, and be used for the study of genetic function verification, the influences of single factor on the adventitious bud of embryonic tip induction, elongation and rooting stage, are researched and compared.The single factors includes seeds soaking time, different kinds of hormones, different concentration of hormone and different concentration of sucrose. By one-way ANOVA and LSD ad hoc test , the results show that, for the embryonic tip adventitious bud induction stage, 12h is the optimal seeds soaking time, 2.0mg·L-1 is the optimal concentration of 6-Benzyl Aminopurine(6-BA), for the embryonic tip adventitious bud...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybean; Induction; Elongation; Rooting; Single factor.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200204
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Extraction and determination of ascorbate and dehydroascorbate from apoplastic fluid of stem of rooted and non-rooted cuttings in relation to the rhizogenesis Braz. J. Plant Physiol.
Zuffellato-Ribas,Katia Christina; Morini,Stefano; Picciarelli,Piero; Mignolli,Francesco.
Starting from semihardwood branches of peach and plum rootstocks (Prunus cerasifera E.) cv. MRS2/5, plum rootstocks (Prunus cerasifera × P. salicina) × (P. cerasifera × P. persica) cv. Ishtarà and olive tree (Olea europaea L.) cv. Frantoio, cuttings were collected and treated with 0 mg.L-1 and 2500 mg.L-1 of indolebutyric acid (IBA) by immersion in the base of the cuttings. After 60 days of planting, the cultivars MRS 2/5, Ishtarà and Frantoio presented a significative difference with relation to the rooting induction by application of the indolebutyric acid (27.5, 47.5 and 41.25 %, respectively), inducing a higher number of roots per cutting without at the same time significantly increasing the length of the roots themselves. The levels of ascorbate and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Olive tree; Peach rootstock; Plum rootstock; Redox potential; Rooting.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000200006
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Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid Chilean J. Agric. Res.
Sánchez-Olate,Manuel; Sáez,Patricia; Cartes,Priscila; Alvarez,Carolina; Ríos,Darcy.
From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results indicate that in the 16:8 h photoperiod induction, the rooting rate with IBA (90%) was significantly higher than that obtained with the strain A477 of A. rhizogenes (67.7%), while with the strain A478 no statistically significant difference was obtained for the same variable (75%). On the other hand, under complete darkness, rooting mediated by IBA (90%) significantly surpassed the results obtained with both...
Tipo: Journal article Palavras-chave: Rooting; European hazelnut.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100013
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Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes Chilean J. Agric. Res.
Sánchez-Olate,Manuel; Sáez,Patricia; Ríos,Darcy.
The in vitro introduction of adult walnut (Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was made between two root induction systems of walnut microshoots of the fourth subculture of adult walnut tissue of an in vitro introduction program previously reinvigorated through traditional grafting. Rhizogenic induction by indole-3-butyric acid (IBA) and Agrobacterium rhizogenes was used. The rhizogenic process was analyzed in two phases for both auxinic (T1: 3 mg L-1 IBA; T2: 5 mg L-1 IBA) and A. rhizogenes inductions (T3: A-477; T4: A-478). The first phase...
Tipo: Journal article Palavras-chave: Rooting; Microshoots; Adult material.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200019
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Rooting of black raspberry with plant growth regulator Ciência Rural
Villa,Fabíola; Stumm,Diego Ricardo; Silva,Daniel Fernandes da; Menegusso,Fernanda Jaqueline; Ritter,Giovana; Kohler,Taís Regina.
ABSTRACT: The framboseira-negra has a potential for cultivation in Brazil, due to the fact that this fruit produces high quantities of fruits, which confer a high quality jelly. However, their propagation is an obstacle to the establishment of commercial plantations. Production and expansion of a fruit is directly linked to the propagation method and the quality of its development. Given the above, the aim of the present study was to evaluate the use of stem cuttings and IBA in asexual propagation of black raspberry. Two experiments being conducted in greenhouse conditions were performed. Stem cuttings were taken from didactic orchard, with matrix plant of four years, which were the experiments. The first experiment was carried out in factorial 3x2, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rubus niveus Thunberg; Stem cutting; IBA; Rooting.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300401
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Roles of arbuscular mycorrhizal fungi on acclimatization of clones of Coffea arabica L. produced by somatic embryogenesis Ciência e Agrotecnologia
Fonseca,Arley José; Tassone,Guilherme Augusto Teixeira; Carneiro,Marco Aurélio Carbone; Carvalho,Gladyston Rodrigues; Carvalho,Carlos Henrique Siqueira; Botelho,Cesar Elias.
ABSTRACT The production of Coffea arabica L. clone seedlings through the somatic embryogenesis technique is one of the alternatives of greatest demand for coffee producers. However, clones can face difficulties related to acclimatization carried out under greenhouse conditions, which can increase the production costs. A tested alternative with promising results is inoculation with arbuscular mycorrhizal fungi (AMF) during the seedling acclimatization process. Thus, this study aimed to promote the improvement of the seedling production process through somatic embryogenesis associated with inoculation with AMF. For the production of the clones, seedlings were used in the phase when they presented four pairs of leaves (Clone 1 “Fruiting red Catucaí” and Clone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propagation; Rooting; Clones; Mycorrhizae; Mineral nutrition.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100222
Registros recuperados: 54
Primeira ... 123 ... Última
 

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