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Registros recuperados: 152 | |
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Nascimento,MS; Berchieri Jr,A; Barbosa,MD; Zancan,FT; Almeida,WAF. |
Este trabalho foi desenvolvido para avaliar, comparativamente, os caldos de enriquecimento Rapapport-novobiocina (RVN), selenito-cistinanovobiocina (SCN) e tetrationato-novobiocina (TN) e os meios para plaqueamento ágar Hektoen (HE), ágar MacConkey (MC), ágar Salmonella-Shigella (SS), ágar verde brilhante (VB) e ágar xilose lisina desoxicolato (XLD), utilizados no isolamento de Salmonella em carcaças de frango e fezes de aves. O procedimento bacteriológico consistiu das etapas de pré-enriquecimento, enriquecimento em caldos seletivos, plaqueamento, testes bioquímicos presuntivos e confirmação sorológica com soros polivalentes anti antígenos somáticos e flagelares de Salmonella.. As fezes foram experimentalmente contaminadas com 8 sorotipos de Salmonella... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Enriquecimento; Plaqueamento; Isolamento; Carcaça de aves; Fezes. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2000000100012 |
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Machado,SCA; Pereira,VLA; Aquino,MHC; Giombeli,A; Rodrigues,DP; Nascimento,ER. |
ABSTRACT The present study investigated the frequency, level of contamination and serotyping of Salmonella strains isolated from broiler flocks in different processing sites and the fulfillment of a Performance Objective (PO) in frozen chicken breasts, as a risk assessment to measure the efficacy of prevention and control programs applied to reduce the risk of Salmonella spp. in raw poultry meat that contribute to reach food safety and public health goals. From 1,800 samples of cloacal swabs, carcasses before and after immersion chilling and frozen breasts derived from 20 broiler flocks slaughtered at two processing plants located in the mid-west and southern regions of Brazil, 278 samples were positive for Salmonella spp. by polymerase chain reaction... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Chicken meat; Salmonella; Serotype; Enumeration; Performance objective (PO). |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100328 |
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Aksoy,A; El Kahlout,KEM; Yardimci,H. |
ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Disinfectants; PCR; Poultry; Resistance; Salmonella. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100301 |
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Biffi,CP; Stefani,LM; Miletti,LC; Matiello,CA; Backes,RG; Almeida,JM; Neves,GB. |
Isolates of Salmonella sp (104) from poultry samples were isolated and serotyped where eleven were identified as Salmonella Typhimurium (ST). ST isolates were phenotypically tested by disk diffusion and minimum inhibitory concentration (MIC). Four genes related to enrofloxacin (GyrA, GyrB, ParC and ParE), two to gentamicin (AadA and AadB) and two to ceftiofur (BlaCMY-2 and AmpC) resistance were searched by PCR. Our results showed ST resistance to all three antibiotics tested (18.1% for ceftiofur, 45.4% for gentamicin, and 18.1% for enrofloxacin) according to the diffusion test. In the MIC test, the ST isolates showed higher levels of resistance (27.2% for ceftiofur, 54.5% for gentamicin, and 18.2% for enrofloxacin). Three resistance genes out of four... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial; Gene; Resistance; Salmonella. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200013 |
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Mesa,D; Lourenço,M; Souza,A; Bueno,A; Pereira,A; Sfeir,M; Santin,E. |
ABSTRACT In broiler production, the litter is reused for consecutives flocks, and it is treated during down time between flocks to reduce its microbial load. Although covering the litter with a plastic canvas is a common litter treatment in the field, there is little scientific information available on its efficacy. The aim of this study was to evaluate the effects of covering broiler litter with a plastic canvas for eight days on litter microbiological, physical, and chemical parameters, and on the intestinal microbiota and immunity of broilers. In the first trial, reused litter from a previous flock was distributed into three treatments, with six replicates each: L1 (negative control, litter free from Salmonella Enteritidis (SE) and Eimeria maxima (EM)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ammonia; Cytokines; Lactobacillus; Macrophages; Salmonella. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400563 |
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Colla,FL; Mion,L; Parizotto,L; Rodrigues,LB; Pilotto,F; Dickel,EL; Nascimento,VP do; Santos,LR dos. |
Salmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most probable number (mMPN) methods, which use microplates, can be adapted for the enumeration of bacteria, saving up time and materials. The aim of the present paper is to assess two mMPN methods for the enumeration of Salmonella sp in artificially-contaminated chicken meat samples. Microplates containing 24 wells (method A) and 96 wells (method B), both with peptone water as pre-enrichment medium and modified semi-solid Rappaport-Vassiliadis (MSRV) as selective... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken meat; Most probable number; Salmonella. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100006 |
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Noujaim,JC; Andreatti Filho,RL; Lima,ET de; Okamoto,AS. |
The expression of immune response as a leukocytic infiltrate by CD4+ and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks fed Lactobacillus spp or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis (SE) was studied using immunohistochemistry. Three hundred and twenty day-of-hatch broiler chicks were divided into four groups of 80 birds each and orally received L. reuteri, L. salivarius, L. acidophilus, or CM. Each group was subdivided into four subgroups of 20 birds each, classified as follows: a subgroup did not receive any oral treatment (negative control), subgroup treated with L. spp or CM, subgroup treated with L. spp or CM and challenged with SE, and subgroup only... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Immunohistochemistry; Lymphocytes CD4+ and CD8+; Probiotic; Salmonella. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300008 |
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Lee,HY; Biswas,D; Ahn,J. |
ABSTRACT This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adhesion; Bacteriophage; Invasion; Salmonella; Toll-like receptor. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400427 |
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Okamoto,AS; Andreatti Filho,RL; Lima,ET; Pereira,REP; Menconi,A; Rocha,TS; Marietto-Gonçalves,GA. |
This study aimed at the antibody production by intestinal mucosa of broilers chicks were orally inoculated with Lactobacillus spp. at one and/or 21 days of age, and subsequently challenged with Salmonella enterica, subspecies enterica, serotype Enteritidis (S. Enteritidis). A total number of 288 drug-free broiler chicks was divided into 6 groups (groups A, B, C, D, E, and F), according to age at Lactobacillus spp. inoculation and SE challenge. The intestinal mucosa immune response was determined as the production of immunoglobulin A against S. Enteritidis, and evaluated by the Enzyme-Linked Immunosorbent Assay (ELISA) technique in intestinal washing fluid. Groups treated with Lactobacillus spp. presented higher IgA production only when the chicks were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicks; IgA; Lactobacillus; Salmonella. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400009 |
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Gama,NMSQ; Berchieri Jr,A; Fernandes,SA. |
This study was carried out to investigate the presence of Salmonella sp in flocks of white laying hens. In different farms, the transport boxes of twelve flocks were inspected at arrival for the presence of Salmonella. Four positive (A, B, L and M) and one negative (I) flocks were monitored at each four weeks using bacteriological examination of cecal fresh feces up to 52 weeks. Birds were also evaluated at 52 weeks, when 500 eggs were taken randomly, and at 76 weeks, after forced molt. Salmonella enterica serovar Enteritidis and S. enterica rough strain were isolated from the transport boxes of the four positive flocks (flocks A, B, L and M). Salmonella sp was not isolated from the transport boxes or from the feces after 76 weeks-old in flock I.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eggs; Feces; Laying hens; Salmonella. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100002 |
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Mandelli,JZA; Ehrhardt,A; Manto,L; Borges,KA; Furian,TQ; Weber,B; Rodrigues,LB; Santos,LR. |
ABSTRACT Antimicrobial resistance is a serious public health problem and Salmonella spp. is highly resistant to antimicrobial agents. Biofilms are important in the food industry due to their formation on products, utensils, and surfaces and the difficulty in their removal. The objective of this study was to assess extended spectrum beta-lactamase (ESBL) production, antimicrobial resistance, and biofilm production of Salmonella isolated from poultry slaughterhouses. Antimicrobial susceptibility was assessed by the disk diffusion assay and ESBL by double diffusion disk assay using the beta-lactamase inhibitor (amoxicillin+clavulanate). The antimicrobials tested were: ampicillin, amoxicillin+clavulanate, aztreonam, ceftazidime, cefotaxime, chloramphenicol,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Drug resistance; ESBL; Salmonella. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200348 |
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Fiorentin,L; Vieira,ND; Barioni Júnior,W; Barros,S. |
Occurrence of food poisoning related to Salmonella-contaminated eggs and chicken meat has been frequent in humans. Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) are included among the most important paratyphoid salmonellae associated with chicken meat and eggs. Elimination of Salmonella at the pre-harvest stage can play a significant role in preventing the introduction of this pathogen into the food chain and consequently in the reduction of food poisoning in humans. Bactericidal bacteriophages may provide a natural, nontoxic, feasible and non-expensive component of the multi-factorial approach for a pre-harvest control of Salmonella in poultry. Five bacteriophages lytic for SE PT4 and ST were obtained from 107 samples of feces of free-range... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacteriophages; Food poisoning; Poultry; Salmonella. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200009 |
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Machado,SCA; Pereira,VLA; Aquino,MHC; Santos,AFM; Rodrigues,DP; Giombelli,A; Nascimento,ER. |
ABSTRACT The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food safety; PFGE; Poultry meat; Salmonella. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100135 |
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Costa,Mônica S. S. de M.; Costa,Luiz A. de M.; Pelá,Adilson; Silva,Cesar J. da; Decarli,Léo D.; Matter,Uilson F.. |
Objetivou-se avaliar o desempenho de quatro sistemas para compostagem de carcaça de aves pelo monitoramento diário da temperatura, análise da presença ou ausência de Salmonella sp., contagem de coliformes totais (CT) e fecais (CF) e reciclagem de nutrientes. Os sistemas avaliados foram: Leira com aeração (LCA), Leira sem aeração (LSA), Composteira com aeração (CCA) e Composteira sem aeração (CSA). O processo de compostagem foi conduzido em dois estágios. No primeiro, as carcaças foram intercaladas com cama de aviário, palha e água. No segundo estágio, a massa de compostagem foi transferida para um pátio onde o material foi revolvido e umedecido semanalmente até a estabilização. Detectou-se a presença de Salmonella sp nos sistemas LSA e CSA ao final do... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Temperatura; Salmonella; Coliformes totais e fecais; Reciclagem de nutrientes. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662006000300023 |
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Pilarski,Fabiana; Tomazelli Júnior,Osmar; Casaca,Jorge de Matos; Garcia,Flávio Roberto Mello; Tomazelli,Ingrid Boesche; Santos,Ieda Rottava dos. |
Amostras de músculo de carpa comum (Cyprinus carpio) e de água de três viveiros fertilizados com dejetos de suínos e de um viveiro em que os peixes foram alimentados com ração comercial para peixes foram analisadas durante seis meses. O objetivo foi observar a presença de bactérias patogênicas, como Salmonella, Staphylococcus aureus e coliformes totais e fecais, em peixes comercializados in natura no município de Chapecó, Santa Catarina. Foram aferidos quinzenalmente, na água dos quatro viveiros, oxigênio dissolvido, pH, turbidez, alcalinidade total, dureza total, amônia, nitrato e ortofosfato, bem como a quantidade de coliformes totais e fecais. A análise microbiológica músculo dos peixes demonstrou ausência de Salmonella em todas as amostras. A contagem... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyprinus carpio; Dejetos suínos; Salmonella; Staphylococcus aureus; Coliformes totais e coliformes fecais. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000200001 |
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Ding,Ke; Wang,Jianping; Liu,Ning; Zhang,Feike. |
ABSTRACT This study aimed to investigate the effect of Artemisia apiacea Hance supplementation on growth performance, cecal opportunistic bacteria, and antimicrobial defense using 120 rabbits. There were four experimental diets containing a control and A. apiacea Hance added at doses of 25, 50, and 75 g kg−1 of feed. The trial lasted for 70 days. The results showed that diets supplemented with A. apiacea Hance improved feed intake, body weight gain, and feed efficiency. Linear and quadratic responses were found between feed intake and herbal meal doses. For cecal opportunistic pathogenic bacteria, compared with the control treatment, the herb decreased cecal C. perfringens, Gram-negative bacteria, and Salmonella spp. by 9.5 to 56.8%. Linear responses of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C. perfringens; Gene expression; Salmonella. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100533 |
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Andreatti Filho, Raphael Lúcio; Fernandes, Sueli Aparecida; Boretti, Lígia Pedroso; Barros, Mércia Rodrigues; Del Bem, Sandro Roberto; Fontana, Adriano; Sampaio, Homero Marcos; Savano, Edson Naoto. |
Salmonella serovars isolated from poultry material from different geographical areas were identified in the 1994-1999 period. A total of73 serovars was isolated, with recognition of21 different serovars distributed in nine serogroups. Enteritidis was the serovar with highest incidence, 46.57%, and was isolated from feed, litter and meat, poultry byproducts and fish meal. Natum had the second highest incidence, 6.84%, and 80% of isolations were made from meat meal. S. pullorum represented 2.73% of the isolations, 100% from brown layers, confirming their susceptibility to infection by this serovar. Of the materials included in the present study, broilers and meat meal had more Salmonella spp., 41.09% and 30.13%, respectively. Among the serovars isolated from... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Poultry; Serovars; Serogroups; Isolation Salmonella; Aves; Sorovares; Sorogrupos; Isolamento. |
Ano: 2001 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3310 |
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Registros recuperados: 152 | |
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