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| Registros recuperados: 94 | |
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| Hervio, Dominique; Chagot, Dominique; Godin, Pierre; Grizel, Henri; Mialhe, Eric. |
| Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the subtrate (cytidine 5' monophosphate). Lead phosphate precipiation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as "dense bodies". Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostrea is discussed. |
| Tipo: Text |
Palavras-chave: Acid phosphate activity; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2755.pdf |
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| Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Peudenier, Isabelle; Grizel, Henri. |
| For determination of ploidy levels in bivalves, three techniques are now routinely used: karyological determination. microfluorimetry and flow cytometry. This paper proposes an alternative technique which is based on determination of the optical density (OD) of stained nuclei using image analysis. A karyological and image analysis comparative experiment conducted on juveniles of Crassostrea gigas (Thunberg) and Ruditapes philippinarum (Adam & Reeve) is described and highlights the advantages of the image analysis technique. Initially developed on C. gigas and R. philippinarum, the image analysis method was easily extended to other bivalve species and to different developmental stages, as demonstrated by two experiments conducted on Ostrea edulis (L.).... |
| Tipo: Text |
Palavras-chave: Estimation bivalve triploidy; Image analysis; Triploid; Genetic; Crassostrea gigas; Oysters. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-3200.pdf |
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| Grizel, Henri; Heral, Maurice. |
| The Japanese oyster (Crassostrea gigas) has been introduced in France on a large scale to replace cultivation of the Portuguese oyster (Crassostrea angulata), affected by a viral disease. The importation took place from 1971 to 1975, with broodstock coming from British Columbia (Canada) and spat from Japan. Good growth rate and success of the natural setting on the French Atlantic coast attested to the success of this implantation, with a production which reached 80000 t by 1976. The precautionary measures associated with this transfer Iimited the implantation of an introduced fauna, 15 years after, to: Balanus amphitrite, Aiptasia pulchella and, on the Mediterranean coast, Undaria pinnatifida and Laminaria japonica. The authors also discuss the possible... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oyster; Introduction; Crassostrea gigas; Huître; Introduction. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2760.PDF |
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| Grizel, Henri. |
| Ce mémento concerne essentiellement l'application de la Directive CEE 91/67 du 28 janvier et des textes qui en découlent relatifs à la protection de la santé des cheptels (Décret 95-1 00 du 26 janvier 1995). Il faut toutefois ne pas oublier que les transferts de coquillages de taille marchande doivent en plus respecter les dispositions destinées à garantir leur salubrité et la santé des consommateurs. A ce jour, deux maladies de mollusques sont obligatoirement contrôlées et soumises à déclaration. Les transferts à l'intérieur des frontières françaises ou entre les pays de l'Union Européenne (U.E.) sont régis par les mêmes réglementations. Le littoral européen est découpé en zones qui ont l'un des statuts suivants : a) agréé ou indemme, c'est-à-dire dans... |
| Tipo: Text |
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| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00446/55729/57381.pdf |
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| Lauret-stepler, Marie; Bourjea, Jerome; Roos, David; Pelletier, Dominique; Ryan, P.g.; Ciccione, Stephane; Grizel, Henri. |
| The green turtle Chelonia mydas is classified as endangered because of global declines over the past few centuries due to human exploitation and habitat destruction, particularly the loss of nesting areas. We used the number of tracks as an indicator of breeding female abundance at their nesting sites to study the seasonality and trends of turtles breeding at 3 islands in the SW Indian Ocean: Europa, Tromelin and Grande Glorieuse, over 20 yr. On Tromelin, tracks were counted along the entire nesting beach, but on Europa and Grande Glorieuse counts were limited to a proportion of the island. Europa and Tromelin exhibited similar seasonal patterns, with a well-defined peak during the wet season (November–February), compared to a dry season peak for Grande... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00018/12917/9874.pdf |
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| Cousserans, François; Bonami, Jean-robert; Comps, Michel; Grizel, Henri. |
| Cell cultures, which are the basis of numerous recent studies in medical pathology, have been, for a few years, of great interest in the studies carried out on the diseases affecting invertebrates. However, such cultures, dealing with marine bivalve molluscs, obtained for the first time by Vago and Chastang (1) and progressively improved since then [(2) to (10)], have not been used in pathological studies yet. As the diseases affecting marine molluscs of economic importance such as oysters are both of great interest and facing specific study difficulties, we tried to adapt oyster cell cultures to the study of the presence, development and cycles of the pathogenic or possibly pathogenic microorganisms affecting Crassostrea, and especially the species gigas,... |
| Tipo: Text |
Palavras-chave: Culture cellule; Parasite; Huîtres; Crassostrea gigas. |
| Ano: 1974 |
URL: http://archimer.ifremer.fr/doc/1974/publication-5907.PDF |
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| Tige, Gilbert; Grizel, Henri; Langlade, Aime; Rabouin, Marie-agnès. |
| Les premiers stades connus du parasite ont été mis en évidence en Bretagne pendant les mois de juillet et d'août (GRIZEL et TIGE, 1977). Suite à ces observations, un essai a été réalisé en 1978 pour savoir si l'infestation avait bien lieu à ces dates ou si elle se faisait antérieurement sous une forme non décelée actuellement et subissant des transformations profondes dans les tissus de l'huître. Les résultats montrent que l'infestations se fait effectivement pendant l'été, à partir du mois de juillet jusqu'à la fin du mois d'août. Elle fut dans l'ensemble faible; les lots les plus infestés sont ceux qui ont séjourné le plus longtemps en zone contaminée. |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Marteilia refringens; Pathologie; Parasite. |
| Ano: 1979 |
URL: http://archimer.ifremer.fr/doc/1979/acte-6098.PDF |
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| Hervio, Dominique; Chagot, D; Godin, P; Grizel, Henri; Mialhe, Eric. |
| Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the substrate (cytidine 5' monophosphate). Lead phosphate precipitation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as 'dense bodies'. Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostreae is discussed. |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00182/29311/28273.pdf |
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| Grizel, Henri. |
| Le département de l'Ille et Vilaine possède dans sa partie nord, une frange littorale d'environ 50 km (vol d'oiseau) depuis le Frémur jusqu'à la limite est de la baie du Mont St Michel et s'étend sur environ 15 km de long en profondeur (vol d'oiseau) sur le bassin de la Rance. Les activités de cette façade maritime concernent de façon saisonnière le tourisme, toute l'année le trafic de marchandises et de passagers et toutes les professions liées directement à l'exploitation et la mise en valeur des produits de la mer. Celles-ci sont représentées par la pêche, l'ostréiculture, la mytiliculture, la pisciculture en eau de mer, l'extraction d'agrégats marins et l'exploitation des algues (fig. 1). |
| Tipo: Text |
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| Ano: 1981 |
URL: http://archimer.ifremer.fr/doc/00046/15684/13086.pdf |
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| Bourjea, Jerome; Lapegue, Sylvie; Gagnevin, L; Broderick, D; Mortimer, J.a.; Ciccione, S; Roos, David; Taquet, Coralie; Grizel, Henri. |
| Patterns of mitochondrial DNA (mtDNA) variation were used to analyse the population genetic structure of southwestern Indian Ocean green turtle (Chelonia mydas) populations. Analysis of sequence variation over 396 bp of the mtDNA control region revealed seven haplotypes among 288 individuals from 10 nesting sites in the Southwest Indian Ocean. This is the first time that Atlantic Ocean haplotypes have been recorded among any Indo-Pacific nesting populations. Previous studies indicated that the Cape of Good Hope was a major biogeographical barrier between the Atlantic and Indian Oceans because evidence for gene flow in the last 1.5 million years has yet to emerge. This study, by sampling localities adjacent to this barrier, demonstrates that recent gene... |
| Tipo: Text |
Palavras-chave: Phylogeography; Mozambique Channel; Mitochondrial DNA; Indian Ocean; Control region; Chelonia mydas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2185.pdf |
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| Grizel, Henri. |
| The notions of pathology and epidemiology in marine aquaculture are relatively new, especially as far as molluscs are concerned, as they've only been used for about thirty years. Although numerous studies have been conducted, and the concepts in these specific fields have evolved, only very few actions are proposed to stop the spreading of a disease. Only a few main mollusc species are cultivated throughout the world and most of them suffered or suffer from epizooties. Today, their pathological history is more solid: several infectious diseases are now known, numerous pathogens or potential pathogens have been described, diverse mortalities have been studied and several types of tumours have been observed. We now live in some cases, as for the flat oyster... |
| Tipo: Text |
Palavras-chave: Aquaculture marine; Pathologie; Conchyliculture; Bivalves; Epidémiologie. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-6580.pdf |
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| Bachere, Evelyne; Chagot, Dominique; Grizel, Henri. |
| There is an ongoing need to isolate and purify ceIls in bivalve mollusc disease research. Many separation techniques differentiate cells solely by a single factor and usually require additives in the medium that may damage live cells. Centrifugai elutriation is a technique that separates particles by size and density simultaneously and can be conducted in physiological media without additives. Living cells can be separated without chemical damage and, because the procedure can be conducted aseptically, cells can be maintained in vitro after elutriation. A description of the technique, methods to determine flow rates and rotor speeds, and a preliminary separation of oyster hyalinocytes is presented |
| Tipo: Text |
Palavras-chave: Bivalve mollusc; Centrifugal elutriation; Cell separation. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3118.pdf |
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| Bourjea, Jerome; Frappier, Julie; Quillard, Mireille; Ciccione, Stephane; Roos, David; Hughes, Georges; Grizel, Henri. |
| Situated in the north of the Mozambique Channel, Mayotte is the easternmost island of the Comoros Archipelago. From 1998 to 2005, Grande Saziley beach was monitored daily for green turtle Chelonia mydas nesting activity. Track surveys were monitored daily on 5 other adjacent beaches. Although nesting occurs throughout the year, nesting seasonality begins at the end of the wet season and intensifies during the dry season to reach an average nesting peak in June. In order to estimate the number of females nesting in the Saziley site and population trends over the study period, incubation success and number of nests per female and per season were estimated at 0.77 (±0.05 SD) and 3.03 (±0.37) respectively. With an average of 1545 nesting turtles per year... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00018/12916/9873.pdf |
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| Registros recuperados: 94 | |
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