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| Registros recuperados: 2.463 | |
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| Ma,Jun; He,Yehua; Hu,Zhongyi; Xu,Wentian; Xia,Jingxian; Guo,Cuihong; Lin,Shunquan; Chen,Chengjie; Wu,Chenghou; Zhang,Junli. |
| Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20-80 fold more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AcSERK3; Autophosphorylation activity; Expression analysis; Prokaryotic expression; Somatic embryogenesis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400009 |
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| Mori,Naoki; Yoshinaga,Kentaro; Tada,Makiko; Wang,Yanhua; Shiseki,Masayuki; Motoji,Toshiko. |
| A unique mutation of the JAK2 gene, V617F, has recently been identified in polycythemia vera, essential thrombocythemia and myeloid metaplasia with myelofibrosis. To determine the relevance of this mutation in other types of hematological neoplasms in Japan, we performed allele-specific polymerase chain reaction analysis on the JAK2 gene. The V617F mutation was detected in one out of 130 myeloid neoplasms, but in none of 114 lymphoid malignancies and four biphenotypic acute leukemias. Although a favorable chromosomal alteration t(8;21)(q22;q22) was observed in one acute myeloid leukemia (AML) patient with the mutation, two courses of chemotherapy resulted in induction failure and short survival. Sequencing of JAK2 cDNA revealed expression of the mutant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: JAK2 gene; V617F mutation; Signal transduction; Acute myeloid leukemia; Lymphoid malignancies. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300006 |
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| Glienke-Blanco,Chirlei; Aguilar-Vildoso,Carlos Ivan; Vieira,Maria Lúcia Carneiro; Barroso,Paulo Augusto Vianna; Azevedo,João Lúcio. |
| During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Endophytes; Genetic variability; Guignardia citricarpa; Mandarin; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200021 |
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| Oliveira,Eder Silva de; Junges,Ângela; Sbaraini,Nicolau; Andreis,Fábio Carrer; Thompson,Claudia Elizabeth; Staats,Charley Christian; Schrank,Augusto. |
| Abstract Cell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation and recycling are essential processes required for cell wall biogenesis; notably, the activity of β-N-acetylglucosaminidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such as Metarhizium anisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role of NAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20 NAGases (MaNAG1 and MaNAG2) and GH3 NAGases (MaNAG3 and MaNAG4) identified in M. anisopliae. While MaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NAGases; GH20 and GH3; Metarhizium; Chitinolytic system; Entomopathogenesis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500843 |
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| Engels,Cibelle; Fuganti-Pagliarini,Renata; Marin,Silvana Regina Rockenbach; Marcelino-Guimarães,Francismar Corrêa; Oliveira,Maria Cristina Neves; Kanamori,Norihito; Mizoi,Junya; Nakashima,Kazuo; Yamaguchi-Shinozaki,Kazuko; Nepomuceno,Alexandre Lima. |
| The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent Dehydration Responsive Element Binding (DREB) gene family has been used to obtain plants with increased tolerance to abiotic stresses. In the present study, the rd29A:AtDREB2A CA gene from Arabidopsis thaliana was inserted into soybean using biolistics. Seventy-eight genetically modified (GM) soybean lines containing 2-17 copies of the AtDREB2A CA gene were produced. Two GM soybean lines (P1397 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arabidopsis; Differential expression; Genetically modified organism; Water deficit. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400015 |
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| Santos,Lígia Ramos dos; Almeida,Jucimara Ferreira Figueiredo; Pimassoni,Lúcia Helena Sagrillo; Morelato,Renato Lírio; Paula,Flavia de. |
| Abstract Genome-wide associations studies (GWAS) are detecting new variants associated with late-onset of Alzheimer’s disease (LOAD), a multifactorial neurodegenerative disorder. The variants rs744373 BIN1, rs11136000 CLU and rs3764650 ABCA7 uncovered by GWAS led to different AD pathways, such as metabolism, trafficking and endocytosis of lipids and inflammation. However, most of the association studies did not replicate these variants with significance. This could be due to a small power effect evident when these variants are tested independently with LOAD. Therefore, we aimed to investigate whether the combination of different variants would additively modify the risk of association with LOAD that is observed in GWAS. We performed an association study... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GWAS variants; APOE; CLU; BIN1; ABCA7. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100113 |
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| Abreu,Flávia Barbosa; Silva,Derly José Henriques da; Marim,Bruno Garcia; Carneiro,Pedro Crescêncio Souza; Juhasz,Ana Cristina Pinto; De Luca,Carina A.C.; Valente,Reginaldo Fialho; Guimarães,Marcelo Almeida. |
| This study presents the minimum number and the best combination of tomato harvests needed to compare tomato accessions from germplasm banks. Number and weight of fruit in tomato plants are important as auxiliary traits in the evaluation of germplasm banks and should be studied simultaneously with other desirable characteristics such as pest and disease resistance, improved flavor and early production. Brazilian tomato breeding programs should consider not only the number of fruit but also fruit size because Brazilian consumers value fruit that are homogeneous, large and heavy. Our experiment was a randomized block design with three replicates of 32 tomato accessions from the Vegetable Germplasm Bank (Banco de Germoplasma de Hortaliças) at the Federal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon esculentum; Genetic resources; Principal components; Repeatability coefficient; Determination coefficient. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100021 |
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| Beljanski,Mirko. |
| The plant-derived anticancer agent PB-100 selectively destroys cancer cells, even when multidrug resistant; yet, it does not inhibit normal (non-malignant) cell multiplication. Testing of PB-100 on sixteen malignant cell lines, several multidrug resistant, as well as on five normal cell lines, confirmed our previous results. Flavopereirine and dihydroflavopereirine, the active principles of PB-100, were chemically synthesized and displayed the same selectivity for tumor cells as the purified plant extract, being active at even lower concentrations. |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100005 |
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| Forni-Martins,Eliana Regina; Martins,Fernando Roberto. |
| Cerrado is the Brazilian name for the neotropical savanna, which occurs mainly in Brazilian Central Plateau, composed of herbaceous-subshrubby and shrubby-arboreal floras, both of which are heliophilous, highly diverse and regionally differentiated. Considering species distribution and chromosome numbers, some authors have proposed that the herbaceous-subshrubby flora of the neotropical savanna is quite old, while the shrubby-arboreal flora is derived from forests, a hypothesis that implies higher chromosome numbers in the savanna than in the forest. If, however, chromosome numbers are similar in the cerrado and in forests, both could be similarly old, indicating that bi-directional flow of flora occurred in the past. This paper presents data on chromosome... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400040 |
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| Mariath,Luiza Monteavaro; Silva,Alexandre Mauat da; Kowalski,Thayne Woycinck; Gattino,Gustavo Schulz; Araujo,Gustavo Andrade de; Figueiredo,Felipe Grahl; Tagliani-Ribeiro,Alice; Roman,Tatiana; Vianna,Fernanda Sales Luiz; Schuler-Faccini,Lavínia; Schuch,Jaqueline Bohrer. |
| Abstract Musicality is defined as a natural tendency, sensibility, knowledge, or talent to create, perceive, and play music. Musical abilities involve a great range of social and cognitive behaviors, which are influenced by both environmental and genetic factors. Although a number of studies have yielded insights into music genetics research, genes and biological pathways related to these traits are not fully understood. Our hypothesis in the current study is that genes associated with different behaviors could also influence the musical phenotype. Our aim was to investigate whether polymorphisms in six genes (AVPR1A, SLC6A4, ITGB3, COMT, DRD2 and DRD4) related to social and cognitive traits are associated with musicality in a sample of children.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musicality; Music; Vasopressin; AVPR1A. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300421 |
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| Crnjac,Josip; Ozretić,Petar; Merkaš,Siniša; Ratko,Martina; Lozančić,Mateja; Korolija,Marina; Popović,Maja; Mršić,Gordan. |
| Abstract X chromosome STR typing has emerged recently as a powerful tool, complementary to autosomal STR typing, in solving complex forensic and missing person cases. Investigator® Argus X-12 is a commercial product that allows co-amplification of 12 X chromosomal markers belonging to four linkage groups (LGs). In this study, we analyzed by capillary electrophoresis blood samples from 100 females and 102 males from a population of northern Croatia. Statistical analysis included calculation of allele and haplotype frequencies, as well as forensic parameters. The most informative marker for the northern Croatia population was DXS10135 with PIC=0.9211 and a total of 27 alleles. The least polymorphic marker was DXS8378 with 6 alleles. The proportion of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: X-STR; Forensic genetics; Investigator® Argus X-12; Population genetics; Northern Croatia. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100080 |
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| Prado,Fernanda Dotti do; Nunes,Tatiana Leite; Senhorini,José Augusto; Bortolozzi,Jehud; Foresti,Fausto; Porto-Foresti,Fábio. |
| The cytogenetic characteristics of Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum and their F1, F2 and backcross hybrids were assessed by using chromosome banding techniques. The diploid number of 56 chromosomes was constant in all species and lineages, with a karyotypic formula containing 20 metacentric, 12 submetacentric, 12 subtelocentric and 12 acrocentric chromosomes. Nucleolar organizer regions (NORs) were identified in two subtelocentric chromosomes in the parents and hybrids, with partial nucleolar dominance in F1 and F2 specimens. Heterochromatic blocks were detected in the terminal and centromeric regions of some chromosomes in all individuals. For parental and hybrid lineages, 18S ribosomal clusters corresponding to NORs and 5S... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Backcrossing; Cytogenetics; Interspecific hybrids; Post-F1 hybrids; Pseudoplatystoma. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100008 |
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| Pucci,Marcela Baer; Nogaroto,Viviane; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo. |
| Abstract Eukaryotic genomes consist of several repetitive DNAs, including dispersed DNA sequences that move between chromosome sites, tandem repeats of DNA sequences, and multigene families. In this study, repeated sequences isolated from the genome of Characidium gomesi were analyzed and mapped to chromosomes in Characidium zebra and specimens from two populations of C. gomesi. The sequences were transposable elements (TEs) named retroelement of Xiphophorus (Rex); multigene families of U2 small nuclear RNA (U2 snRNA); and histones H1, H3, and H4. Sequence analyses revealed that U2 snRNA contains a major portion corresponding to the Tx1-type non-LTR retrotransposon Keno, the preferential insertion sites of which are U2 snRNA sequences. All histone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mobile DNA; Histones; Karyotype evolution; SnRNA; WZ/ZZ. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400585 |
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| Sofia,Silvia H.; Galindo,Bruno A.; Paula,Francine M.; Sodré,Leda M.K.; Martinez,Cláudia B.R.. |
| In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loricariid fish; Genetic diversity; RAPD; Siluriformes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027 |
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| Registros recuperados: 2.463 | |
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