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| Registros recuperados: 87 | |
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| Ferreira,Kátia; Torres,Giovana Augusta; Carvalho,Isabelle Vilela de; Davide,Lisete Chamma. |
| The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fluorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confirming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalaria; Cytogenetics; Cytomixis; Meiosis; Pollen grain viability. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200012 |
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| Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Souza Sobrinho,Fausto; Davide,Lisete Chamma. |
| Breeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germplasm bank; Cytogenetics; Flow cytometry; Forage. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000100008 |
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| Conterato,Ionara Fatima; Dall'Agnol,Miguel; Schifino-Wittmann,Maria Teresa; Janke,Aline. |
| Twenty eight natural populations of Trifolium riograndense Burkart, an important forage legume from native pastures of the state of Rio Grande do Sul, Brazil, were evaluated for genetic diversity with eight Simple Sequences Repeats (SSR) markers. Chromosome numbers were also determined. The eight markers were polymorphic, with 35 alleles and an average of 4.37 alleles per locus, and Polymorphism Information Content (PIC) between 0.48 and 0.80. Group analysis based on Jaccard´s similarity coefficient separated the 28 accessions in nine groups, with an average genetic similarity of 0.44, indicating a high genetic variability among the populations. No evident relation between genetic distance and geographical origin was detected. The chromosome number of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Native clover; Genetic diversity; Cytogenetics; Native pastures. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000200005 |
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| Zappelini,Julia; Souza,Luiz Gustavo; Guerra,Miguel Pedro; Pescador,Rosete. |
| ABSTRACT Although karyotype features are useful data for evolutionary studies, cytogenetic data in Bambusoideae are mainly based only on chromosome counts. The first comparative cytogenetic analysis of three Neotropical woody bamboo species - Guadua chacoensis, G. angustifolia and Chusquea tenella - was undertaken based on new and reviewed chromosome counts, CMA/DAPI double staining, fluorescent in situ hybridization (FISH) with 35S and 5S rDNA probes, and genome size estimation. The two species of Guadua were found to have 2n = 46 chromosomes, while the first record for C. tenella was 2n = 44. Only one pair of CMA+/DAPI- was detected on the terminal region of metacentric chromosomes in all three species. Likewise, one pair of 5S and 35S rDNA sites was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chusquea; Cytogenetics; Diploidization; Evolution; Guadua; Polyploidy. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400673 |
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| Techio, Vânia Helena; UNC; Davide, Lisete Chamma; UFLA; Souza, Tatiana Matioli; 3Bios Consultoria e Serviços Ambientais Ltda; Pereira, Antônio Vander; EMBRAPA-MG. |
| Avaliou-se o complemento cromossômico de 16 acessos de Pennisetum disponíveis na Coleção de Germoplasma da Embrapa Gado de Leite – Juiz de Fora, Estado de Minas Gerais. Para a avaliação citogenética, utilizou-se a técnica de esmagamento e coloração com Feulgen. Para quatro acessos foram obtidas metáfases com 2n = 28 cromossomos, confirmando sua classificação como P. purpureum. A origem híbrida P. purpureum x P. glaucum foi constatada em oito acessos, os quais apresentaram 2n = 21. Em um acesso identificado como híbrido entre P. purpureum x P. glaucum, foram observadas metáfases com 2n = 54, permitindo inferir que não se trata de um híbrido interespecífico triplóide. Para outros três acessos avaliados, também foram observadas células com 2n = 54, embora... |
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Palavras-chave: 2.00.00.00-6 Ciências Biológicas Pennisetum; Citogenética; Germoplasma; Híbridos interespecíficos. Pennisetum; Cytogenetics; Germplasm; Interspecific hybrids. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5016 |
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| AMORIM,MARÚCIA I. M.; MERGLER,DONNA; BAHIA,MARCELO O.; DUBEAU,HÉLÈNE; MIRANDA,DANIELA; LEBEL,JEAN; BURBANO,ROMMEL R.; LUCOTTE,MARC. |
| The mercury rejected in the water system, from mining operations and lixiviation of soils after deforestation, is considered to be the main contributors to the contamination of the ecosystem in the Amazon Basin. The objectives of the present study were to examine cytogenetic functions in peripheral lymphocytes within a population living on the banks of the Tapajós River with respect to methylmercury (MeHg) contamination, using hair mercury as a biological indicator of exposure. Our investigation shows a clear relation between methylmercury contamination and cytogenetic damage in lymphocytes at levels well below 50 micrograms/gram, the level at which initial clinical signs and symptoms of mercury poisoning occur. The first apparent biological effect with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian Amazon; Mercury; Mitotic Index; Cytogenetics. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000400004 |
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| RAMOS,ROSEMAR S. L.; VALE,WILLIAM G.; ASSIS,FÁTIMA L.. |
| A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and spleen cells, from D. prymnolopha and D. leporina. The diploid number was 64/65 for all samples. The karyotypes showed similarity, and chromosomal polymorphism was not detected by Giemsa conventional staining and G banding. The constitutive heterochromatin distribution at the pericentromeric region of all the chromosomes was similar in all species. D. prymnolopha, D. leporina and Dasyprocta sp. presented variation in the heterochromatical block... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Hystricognathi; Dasyprocta; Karyotype. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000100007 |
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| Carvalho de Souza,Patrícia; Salim Khayat,André; Chamon Seligmann,Igor; Rodríguez Burbano,Rommel Mario. |
| The collared peccary (Tayassu tajacu) is widely distributed over the American continent, being found from the south of the USA to the north of Argentina. In Brazil, it is spread all over the country, being one of the potential species to be raised in captivity. Therefore, the cytogenetic techniques could be a potential tool for reproductive monitoring of animals raised in captivity, mainly when destined for commercial purposes. This study had the objective of determining the chromosome number of two populations raised in captivity and characterizing them by GTG banding. For this purpose, an analysis was made of mitotic metaphases obtained from lymphocyte cultures made from blood samples of 11 animals, six of which from the Northeast and five from the North... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cytogenetics; GTG Banding; Tayassuidae; Suidae; Pigs. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200007 |
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| QUINDERÉ,YEDA R. S. D; LOURENÇO,LUCIANA B; ANDRADE,GILDA V; TOMATIS,CRISTIAN; BALDO,DIEGO; RECCO-PIMENTEL,SHIRLEI M. |
| We investigated the NOR distribution in ten populations of Physalaemus cuvieri from different regions of Brazil and Argentina. A high variability in NOR pattern was observed and provided a useful tool in grouping several populations. The specimens from the state of Tocantins, northern Brazil, could easily be distinguished from all the other analyzed populations, since its karyotype presented NORs in the chromosome pairs 1,3,4 and 10 (and sometimes also in chromosome 5), and several pericentromeric C-bands. A NOR-site in chromosome 9 characterized three populations from the northeastern region of Brazil. Interestingly, the P. cuvieri populations located in opposite extremes of the geographic distribution had, as a fixed condition, the presence of NORs in 8q... |
| Tipo: Journal article |
Palavras-chave: Anura; Chromosome; Cytogenetics; NOR; Physalaemus; Polymorphic variations; Polytypic variations. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100008 |
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| Registros recuperados: 87 | |
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