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| Registros recuperados: 78 | |
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| Pereira,S.C.L.; Vanetti,M.C.D.. |
| We aimed to evaluate the potential virulence of Klebsiella isolates from enteral diets in hospitals, to support nosocomial infection control measures, especially among critical-care patients. Phenotypic determination of virulence factors, such as capsular expression on the external membrane, production of aerobactin siderophore, synthesis of capsular polysaccharide, hemolytic and phospholipase activity, and resistance to antibiotics, which are used therapeutically, were investigated in strains of Klebsiella pneumoniae and K. oxytoca. Modular industrialized enteral diets (30 samples) as used in two public hospitals were analyzed, and Klebsiella isolates were obtained from six (20%) of them. The hypermucoviscous phenotype was observed in one of the K.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Klebsiella; Enteral diets; Pathogenicity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900782 |
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| Huang,L.; Liu,X.; Li,J.L.; Chang,J.L.; Liu,G.C.; Yu,X.F.; Zhang,W.Y.. |
| An enterovirus 71 (EV71) vaccine for the prevention of hand, foot, and mouth disease (HMFD) is available, but it is not known whether the EV71 vaccine cross-protects against Coxsackievirus (CV) infection. Furthermore, although an inactivated circulating CVA16 Changchun 024 (CC024) strain vaccine candidate is effective in newborn mice, the CC024 strain causes severe lesions in muscle and lung tissues. Therefore, an effective CV vaccine with improved pathogenic safety is needed. The aim of this study was to evaluate the in vivo safety and in vitro replication capability of a noncirculating CVA16 SHZH05 strain. The replication capacity of circulating CVA16 strains CC024, CC045, CC090 and CC163 and the noncirculating SHZH05 strain was evaluated by cytopathic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coxsackievirus A16; Pathogenicity; Circulating strain; Non-circulating strain. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500420 |
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| Bellato,Cláudia de M.; Garcia,Andréia K.M.; Mestrinelli,Fabiana; Tsai,Siu M.; Machado,Marcos A.; Meinhardt,Lyndel W.. |
| An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2DE; Callus; Pathogenicity; Protein; Xylella-citrus interaction. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012 |
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| Chaves,Guilherme Maranhão; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo. |
| In order to evaluate the pathogenicity of yeasts of medical importance, 15 strains stocked in mineral oil at the URM Culture Collection of the Department of Mycology, Biological Sciences Centre, Federal University of Pernambuco and 15 fresh strains isolated from AIDS patients interned at the Clinical Hospital, Centre of Health Sciences, Federal University of Pernambuco, were selected. As pathogenicity characteristics, the ability to grow at 37ºC and production of phospolipase and proteinase were tested. All samples grew at 37ºC and exhibited proteinase activity. However when tested on solid medium, the proteinase activity was negative. Among 15 stocked samples, 13 (86.66%) were phospholipase positive and two (13.34%) were phospholipase negative. From the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yeasts; Pathogenicity; Temperature; Phospholipase; Proteinase. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300003 |
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| Arslan-Aydoğdu,Elif Özlem; Kimiran,Ayten. |
| ABSTRACT Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Legionella pneumophila; Pathogenicity; Virulence of environmental isolates. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100189 |
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| Yong,H.Y.; Bakar,F.D.A.; Illias,R.M.; Mahadi,N.M.; Murad,A.M.A.. |
| The mitogen-activated protein (MAP) kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cgl-SLT2 open-reading frame and its promoter and terminator regions, was isolated via DNA walking and cloned. To analyze gene function, a gene disruption cassette containing hygromycin-resistant gene was constructed, and Cgl-SLT2 was inactivated via gene deletion. Analysis on Cgl-slt2 mutant revealed a defect in vegetative growth and sporulation as compared to the wild-type strain. When grown under... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colletotrichum gloeosporioides; MAP kinase; Pathogenicity; Appressorium. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400031 |
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| Neves,Rejane Pereira; Magalhães,Oliane Maria Correia; Silva,Merivânia Lucas da; Souza-Motta,Cristina Maria de; Queiroz,Lusinete Aciole de. |
| This study investigated the ocurrence of Malassezia species in clinically healthy students and with macules with a slight fawn discoloration and characterized the isolates as to the pathogenicity factors such as growth at 37ºC, lipase, phospholipase and protease detection. Clinical samples were collected from different body sites of one hundred students of different ages and both sexes. The samples, obtained by scrapping the skin surface and the scalp, were treated with potassa and cultured. Cultures were obtained in Petri dishes containing Sabouraud agar medium added of olive oil, incubated at room temperature and at 37ºC. Culture identifications were based in their morphological and physiological properties. Lipase, phospholipase and protease detection... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Malassezia furfur; M. simpodialis; Pathogenicity. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200003 |
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| Reis,Andre L.S.; Montanhini,Maike T.M.; Bittencourt,Juliana V.M.; Destro,Maria T.; Bersot,Luciano S.. |
| Bacillus cereusis an ubiquitous, spore-forming bacteria that can survive pasteurization and the majority of the heating processes used in the dairy industry. Besides, it is a pathogen responsible for different types of food poisoning. One type of foodborne disease caused by B.cereusis the diarrheal syndrome, which is caused by the ingestion of vegetative cells producing toxins in the small intestine. One virulence factor for the diarrheal syndrome is the toxin hemolysin BL (HBL), a three-component protein formed by the L1, L2 and B components. In order to evaluate the presence of diarrheal strains isolated from milk and dairy products, 63 B. cereus isolates were obtained from 260 samples of UHT milk, pasteurized milk and powdered milk, sold in commercial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Dairy products; Bacillus cereus; Pathogenicity; Detection. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400024 |
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| Orsi,M.A; Doretto Jr,L; Camillo,S.C.A; Reischak,D; Ribeiro,S.A.M; Ramazzoti,A; Mendonça,A.O; Spilki,F.R; Buzinaro,M.G; Ferreira,H.L; Arns,C.W. |
| In 2003, Brazil was recognized as a pathogenic Newcastle Disease Virus (NDV) strain-free country for commercial poultry. This research was conducted in Brazil between December 2003 and March 2005 to verify the maintenance of this virulent NDV-free status. Serum samples from 5,455 flocks for commercial poultry farms were collected, comprising 81,825 broiler chickens. The farms were located in nine states of the country, grouped in three geographic regions. Serological evidence of NDV infection was detected in 28.8% of the surveyed farms. However, all fifteen viruses isolated and identified as Newcastle Disease Virus (NDV) were characterized as nonpathogenic strains, based on the Intracerebral Pathogenicity Index. These results showed that Brazil preserves... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Newcastle Disease Virus; Pathogenicity; Poultry; Biological characterization. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200017 |
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| Armesto,Cecilia; Maia,Fernanda Gonçalves Martins; Abreu,Mário Sobral de; Figueira,Antonia dos Reis; Silva,Bruno Marques da; Monteiro,Fernando Pereira. |
| Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colletotrichum gloeosporioides; Fluorescent proteins; Pathogenicity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300050 |
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| Orsi,M.A; Doretto Jr,L; Camillo,S.C.A; Reischak,D; Ribeiro,S.A.M; Ramazzoti,A; Mendonça,A.O; Spilki,F.R; Buzinaro,M.G; Ferreira,H.L; Arns,C.W. |
| This study was carried out during 2002/2003, aiming to determine the prevalence of virulent Newcastle disease virus strains (NDV) in Brazilian commercial poultry farms. Clinical samples were obtained from the Southeastern, Southern and Central-Western regions, which comprise the main area of the Brazilian poultry production. Serum samples and tracheal and cloacal swabs of 23,745 broiler chickens from 1,583 flocks, including both vaccinated chickens and those with no vaccination information, were tested for NDV using a diagnostic ELISA kit. The seropositivity was 39.1%, and the isolation percentage by flock varied from 1.0 to 7.6%, and by region from 6.5 to 58.4%. Higher isolation rates (74.3-83.3%) were obtained after three passages in embryonated chicken... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Newcastle Disease Virus; NDV-free status; Pathogenicity; Broiler chickens; Biological characterization. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200014 |
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| Carvalho,Daiane; Finkler,Fabrine; Grassotti,Tiela Trapp; Kunert Filho,Hiran Castagnino; Lima,Francisco Esmaile de Sales; Soares,Beatriz Dugaich; Rossato,Juliana Marzari; Cunha,Augusto César da; Brito,Kelly Cristina Tagliari de; Brito,Benito Guimarães de. |
| The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (>75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Resistance; Antimicrobials; Pathogenicity; Poultry.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701249 |
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| Registros recuperados: 78 | |
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