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| Registros recuperados: 97 | |
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| Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV. |
| Este trabalho teve como objetivo avaliar níveis de AIB no enraizamento in vitro de microestacas de mamoeiro (Carica papaya L. ‘Tainung 01’). Ápices caulinares de plantas com 120 dias de idade foram estabelecidos e avaliados em meio MS, suplementado com complementos orgânicos constituídos de sacarose a 87,64 mM, mio-inositol a 555,06 µM, tiamina-HCl a 0,30 µM, piridoxina-HCl a 2,43 µM, ácido nicotínico a 4,06 µM e 0; 0,74; 1,48; 2,96 e 5,92 µM de AIB, em delineamento inteiramente casualizado, com quatro repetições e cinco tubos de ensaio por parcela. Após 30 dias, avaliou-se a percentagem de enraizamento, o crescimento das microestacas, o comprimento da maior folha e a massa de calo. Observou-se que o maior percentual estimado de enraizamento ocorreu com a... |
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Palavras-chave: 5.01.00.00-9 Agronomia Carica papaya; Auxina; Micropropagação; Cultura de tecidos Fitotecnia Carica papaya; Auxin; Micropropagation; Tissue culture. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/866 |
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| Castro, Juliana Pereira de; Universidade Federal da Paraíba; Araújo, Emmanuelle Rodrigues; Universidade Federal da Paraíba - UFPB; Rêgo, Mailson Monteiro do; Universidade Federal da Paraíba - UFPB; Rêgo, Elizanilda Ramalho do; Universidade Federal da Paraíba - UFPB. |
| This work aimed to promote in vitro seed germination and disinfestation of the sweet cactus. Seeds were submerged in alcohol at 70% for 1 min. and then treated with sodium hypochlorite solution at different concentrations: 0.0; 0.5; 1.0 and 1.5%. After this treatment, the seeds were washed four times in distillated, deionized and autoclaved water. Seeds were inoculated in MS medium with different concentrations of sucrose (0.0, 2.5, 5.0, 7.5 and 10.0 g L-1). The experimental design was completely randomized in a factorial experiment 4 x 5, with five replicates for treatments. Contamination, germination and seedling growth were evaluated. The results of the analysis of variance indicate that there were no significant interactions among factors. There was no... |
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Palavras-chave: 5.01.00.00-9 Cactaceae; Forage; Tissue culture 5.01.00.00-9 Cactaceae; Forage; Tissue culture. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6275 |
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| Brondani, Gilvano Ebling; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Araujo, Marla Alessandra; Universidade Federal de Lavras; Alcântara, Berenice Kussumoto; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Carvalho, Janice Guedes; Universidade Federal de Lavras; Gonçalves, Antonio Natal; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Almeida, Marcilio de; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP). |
| The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The... |
| Tipo: Pesquisa com fatores controlados |
Palavras-chave: 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15143 |
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| Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Rodrigues, Filipe Almendagna; Universidade Federal de Lavras; Villa, Fabiola; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras. |
| O objetivo deste trabalho foi determinar a fonte de silício que proporcione melhor crescimento em plântulas de Cattleya loddigesii. Plântulas oriundas de sementes germinadas in vitro com aproximadamente 1,0 cm de comprimento foram inoculadas em tubos de ensaio contendo 15 mL de meio de cultura Knudson C modificado, acrescido de silicato de sódio (0, 5, 10 e 20 mg L-1) e silicato de potássio (0, 5, 10 e 20 mL L-1), em todas as combinações possíveis. O meio de cultura teve seu pH ajustado para 5,8 ± 0,1 e solidificado com 5 g L-1 de ágar antes do processo de autoclavagem a 121°C e 1 atm por 20 min. O cultivo foi mantido em sala de crescimento com irradiância de 35 µmol m-2 s-1, temperatura de 25 ± 1°C e... |
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Palavras-chave: 5.01.03.00-8 Cattleya loddigesii; Meio de cultura; Orchidaceae; Cultura de tecidos Fitotecnia/Biotecnologia Cattleya loddigesii; Culture medium; Orchidaceae; Tissue culture. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6281 |
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| PáDUA,MARLÚCIA S.; PAIVA,LUCIANO V.; LABORY,CLAÚDIA R.G.; ALVES,EDUARDO; STEIN,VANESSA C.. |
| ABSTRACTOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses inE.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryogenic potential; Growth regulators; Tissue culture; Transmission electron microscopy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401545 |
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| Braga,Virgínia F.; Mendes,Giselle C.; Oliveira,Raphael T.R.; Soares,Carla Q.G.; Resende,Cristiano F.; Pinto,Leandro C.; Santana,Reinaldo de; Viccini,Lyderson F.; Raposo,Nádia R.B.; Peixoto,Paulo H.P.. |
| This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC50 = 169.3 µg mL-1. HF showed a non-dose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Biodiversity conservation; Biological effects; Natural products. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100014 |
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| GHATPANDE,SATISH; SHAFIQ,SAIYID; SIDDIQUI,M.A.Q.. |
| Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such studies, we examined the expression pattern of vMLC2 in chicken embryos at various stages and analyzed the effect of antisense oligonucleotide-mediated interference of vMLC2 function in cultures of whole embryos. Our results showed vMLC2 to be a specific marker for ventricular chamber throughout chicken embryonic development and antisense vMLC2 treatment of primitive streak stage (stage 4) embryos to produce pronounced dilation of heart tube with severe... |
| Tipo: Journal article |
Palavras-chave: Cardiogenesis; Cardiomyopathy; Myosin light chains; Tissue culture. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009 |
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| Carvalho,Milene Alves de Figueiredo; Paiva,Renato; Stein,Vanessa Cristina; Herrera,Raírys Cravo; Porto,Jorge Marcelo Padovani; Vargas,Daiane Peixoto; Alves,Eduardo. |
| This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passifloraceae; Callogenesis; Electromicroscopy; Optic microscope; Tissue culture. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851 |
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| Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz. |
| Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Organogenesis; Anatomy. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013 |
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| Takemori,N. K.; Marschner,R.; Quoirin,M.; Bona,C.; Zanette,F.. |
| In order to achieve adventitious shoot regeneration from young tissues of Racosperma mangium, several combinations of growth regulators were tested. Kinetin (KIN) and a -naphthalenacetic acid (NAA), thidiazuron (TDZ) and NAA or indole-3-butyric acid (IBA), 4-CPPU and 2,4-dichlorophenoxyacetic acid (2,4-D) were added to basic medium composed of MS salts, MS vitamins and 3% sucrose. Sections of hypocotyls and cotyledons of young seedlings were cultivated on these media. Calli developed on different media and, after two to three months, some of them regenerated organized structures. Histological studies revealed hypertrophy and hyperplasy of medular and cortical parenchyma cells of hypocotyl and cotyledon parenchyma. Formation of meristematic cells and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 4-CPPU; Leguminosae; TDZ; Tissue culture. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100008 |
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| Rojas-Lorz,Laura; Arrieta-Espinoza,Griselda; Valdez-Melara,Marta; Pereira,Luiz Filipe Protasio; Gatica-Arias,Andrés. |
| Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffee; Silver nitrate; Somatic embryos; Plant growth regulators; Tissue culture. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100408 |
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| Paiva Neto,Vespasiano Borges de; Paiva,Renato; Furtado,Deborah E.. |
| With the objective to investigate the in vitro behavior of salix (Salix humboldtiana Willdenow), leaf and nodal segments were inoculated in WPM medium supplemented with NAA and IBA. In leaf explants, the presence of these growth regulators, isolated or in combination, induced the formation of adventicious roots. Root hairs were only observed when NAA was present. In nodal explants, while the combination of 2,68 µM ANA + 2,46 µM IBA induced bud growth, the other treatments (4,92 µM IBA; 2,68µM ANA + 4,92 µM IBA; 5,37 µM ANA + 4,92 µM IBA; 5,37µM ANA) induced the formation of adventicious roots. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Salix; Adventicious roots; Root hairs. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100009 |
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| Registros recuperados: 97 | |
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