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Influence of low-protein diet with different levels of amino acids on laying hen performance, quality and egg composition Anais da ABC (AABC)
ALAGAWANY,MAHMOUD; EL-HINDAWY,MOHAMED M.; EL-HACK,MOHAMED E. ABD; ARIF,MUHAMMAD; EL-SAYED,SABRY ABD.
Abstract The present study aimed to investigate the consequences of dietary levels of crude protein (CP) and total sulfur amino acids (TSAA, Met+Cys) on productive performance, egg quality and egg components for Lohmann Brown laying hens through 18-34 weeks of age. A 2×3 factorial design experiment was conducted using two levels of CP (16 and 18 %) and three levels of Met+ Cys (0.67, 0.72 and 0.77 %). A total of 144 Lohmann Brown laying hens at 18 weeks of age were randomly divided into 6 treatment groups. The best values of final body weight and body weight change were recorded by the hens fed 18 % CP diet. Increasing dietary CP up to 18 % accompanied with the best (P> 0.01) feed conversion, egg weight and egg mass. The combination between low-CP diet...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Layers; Low-protein diet; Amino acids; Production; Egg quality; Blood.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100712
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Performance and egg quality of laying hens fed with dietary levels of digestible lysine and threonine Arq. Bras. Med. Vet. Zootec.
Figueiredo,G.O; Bertechini,A.G; Fassani,E.J; Rodrigues,P.B.; Brito,J.Á.G; Castro,S.F.
The present study was developed using 864 Hy-Line W36 laying hens (42 to 58 weeks of age) with a randomized experimental design in a 4x3 factorial arrangement, with five replicates and 12 birds per experimental unit and 4 evaluation periods of 28 days each. The digestible lysine levels studied were 0.675%, 0.743%, 0.811% and 0.879%, and the digestible threonine levels were 0.542%, 0.596% and 0.650%. The performance and egg quality was evaluated. There were no significant effects on the egg production, specific gravity, albumen and yolk percentage and total solids in albumen and yolk for birds submitted to the different dietary digestible lysine and threonine levels. There was significant interaction between these amino acids on the egg shell percentage and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Laying hens; Crude protein; Haugh unit.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300028
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Role of Natural Antioxidants in the Modulation of Plasma Amino Acid Pattern in Rats Exposed to Hemic Hypoxia BABT
Al-Rasheed,Nouf Mohamed; Fadda,Laila Mohamed; Al-Rasheed,Nawal Mohamed; Attia,Hala; Ali,Hanaa Mahmoud; El-Agami,Hanaa.
ABSTRACTThe aim of this work was to investigate whether the free radical scavengers, L-arginine (L-arg) and/or carnosine, either alone, or in combination would modulate tissue injury induced by hypoxia by measuring Fischer's ratio [concentrations of branched chain amino acids (BCAAs)/aromatic amino acids]. Decreased Fischer's ratios and increased malondialdehyde (MDA) led to pathogeneses of many diseases. Rats were injected with sodium nitrite (60 mg/kg) to establish hypoxia. They were treated with L-arg, (200 mg/ kg) and/or carnosine (200 mg/ kg) and their combination 24 and 1 h prior to sodium nitrite intoxication. The results revealed that hypoxia significantly decreased hemoglobin, arginine, citrulline and proline and increased sLDH, MDA , ammonia ,...
Palavras-chave: Natural antioxidants; Amino acids; Carnosine; L-arginine; Fisher's ratio; Urea.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500741
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Alterations in proteins and amino acids of the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense in response to industrial wastewater pollution BABT
El-Sheekh,Mostafa Mohamed; El-Otify,Ahmed Mohamed; Saber,Hani.
The effect of industrial wastewater on the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense was investigated. The data showed that P. limnetica was more sensitive to pollution than A. wisconsinense. The treatments with different levels of wastewater exerted pronounced reductions in protein and amino acids content. SDS-PAGE analysis revealed that the cyanobacteria grown in the industrial wastewater showed induction in the synthesis of certain polypeptides and repression of others. The treatments of P. limnetica with wastewater stimulated the appearance of six protein bands with molecular masses of 28, 30, 31, 32, 58 and 97 kDa. The same treatments caused the disappearance of 20, 38 and 56 kDa. The structural protein pattern of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Anabaena; Electrophoretic patterns; Nile cyanobacteria; Protein; Pseudanabaena; Wastewater.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400022
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Compositional changes of proteins and amino acids in germinating coffee seeds BABT
Shimizu,Milton Massao; Mazzafera,Paulo.
Endosperm is the main reserve tissue in coffee seeds. Coffee (Coffea arabica L.) seeds were germinated for six weeks and qualitative and quantitative changes in amino acids and proteins were investigated. The total content of free amino acids were reduced during germination, however, protein content remained constant. SDS-PAGE profiles showed that legumin-like proteins became less stained in the last weeks. Asparagine, glutamic acid, aspartic acid, alanine and lysine were the major free amino acids, although serine and glutamine were also significant. Except for tyrosine, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed glutamic acid/glutamine and glycine as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Coffea arabica; Endosperm; Germination; Protein; Seeds.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300003
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Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species BJMBR
Tiwari,F.; Singh,D.K..
Adult Lymnaea acuminata (average length 20-22 mm) were collected locally from lakes and low-lying submerged fields from Gorakhpur. The chemoattraction studies were made in round glass aquaria measuring 30 cm in diameter and filled to a depth of 10 mm with 500 ml dechlorinated tap water. Each aquarium was divided into four concentric zones. At the starting time of the assay 10 snails were placed on the circumference of outermost zone 0. Snail attractant pellets (SAP) were added simultaneously in the center of central zone 3. SAP of different amino acids were prepared at concentrations of 10, 20, 50, 80 and 100 mM/2% agar solution and, subsequently, spread to a uniform thickness of 5 mm. After cooling, SAP were cut in small pieces of 5 mm in diameter....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Snails; Lymnaea acuminata; Fasciola; Amino acids; Attraction.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400016
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Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway BJMBR
Oliveira,I.C.; Brenner,E.; Chiu,J.; Hsieh,M.-H.; Kouranov,A.; Lam,H.-M.; Shin,M.J.; Coruzzi,G..
We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana. We have begun to investigate the regulatory mechanisms controlling nitrogen assimilation into these amino acids in plants using molecular and genetic approaches in Arabidopsis. The synthesis of the amide amino acids glutamine and asparagine is subject to tight regulation in response to environmental factors such as light and to metabolic factors such as sucrose and amino acids. For instance, light...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Carbon; Ammonium; Nitrogen assimilation; Gene expression; Arabidopsis thaliana.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500003
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Thyroid peroxidase activity is inhibited by amino acids BJMBR
Carvalho,D.P.; Ferreira,A.C.F.; Coelho,S.M.; Moraes,J.M.; Camacho,M.A.S.; Rosenthal,D..
Normal in vitro thyroid peroxidase (TPO) iodide oxidation activity was completely inhibited by a hydrolyzed TPO preparation (0.15 mg/ml) or hydrolyzed bovine serum albumin (BSA, 0.2 mg/ml). A pancreatic hydrolysate of casein (trypticase peptone, 0.1 mg/ml) and some amino acids (cysteine, tryptophan and methionine, 50 µM each) also inhibited the TPO iodide oxidation reaction completely, whereas casamino acids (0.1 mg/ml), and tyrosine, phenylalanine and histidine (50 µM each) inhibited the TPO reaction by 54% or less. A pancreatic digest of gelatin (0.1 mg/ml) or any other amino acid (50 µM) tested did not significantly decrease TPO activity. The amino acids that impair iodide oxidation also inhibit the TPO albumin iodination activity. The inhibitory amino...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid; Thyroperoxidase; Amino acids; Iodide oxidation; Lactoperoxidase.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300015
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Effect of Walker 256 tumor growth on intestinal absorption of leucine, methionine and glucose in newly weaned and mature rats BJMBR
Gomes-Marcondes,M.C.C.; Honma,H.N.; Areas,M.A.; Cury,L..
In tumor-bearing rats, most of the serum amino acids are used for synthesis and oxidation processes by the neoplastic tissue. In the present study, the effect of Walker 256 carcinoma growth on the intestinal absorption of leucine, methionine and glucose was investigated in newly weaned and mature rats. Food intake and carcass weight were decreased in newly weaned (NT) and mature (MT) rats bearing Walker 256 tumor in comparison with control animals (NC and MC). The tumor/carcass weight ratio was higher in NT than in MT rats, whereas nitrogen balance was significantly decreased in both as compared to control animals. Glucose absorption was significantly reduced in MT rats (MT = 47.3 ± 4.9 vs MC = 99.8 ± 5.3 nmol min-1 cm-1, Kruskal-Wallis test,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Walker 256 tumor; Intestinal absorption; Amino acids; Glucose; Aging.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000017
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Are high-lysine cereal crops still a challenge? BJMBR
Ferreira,R.R.; Varisi,V.A.; Meinhardt,L.W.; Lea,P.J.; Azevedo,R.A..
The essential amino acids lysine and threonine are synthesized in higher plants via a pathway starting with aspartate that also leads to the formation of methionine and isoleucine. Lysine is one of most limiting amino acids in plants consumed by humans and livestock. Recent genetic, molecular, and biochemical evidence suggests that lysine synthesis and catabolism are regulated by complex mechanisms. Early kinetic studies utilizing mutants and transgenic plants that over-accumulate lysine have indicated that the major step for the regulation of lysine biosynthesis is at the enzyme dihydrodipicolinate synthase. Despite this tight regulation, recent strong evidence indicates that lysine catabolism is also subject to control, particularly in cereal seeds. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lysine; Threonine; Aspartate; Nutritionally improved crops; Methionine; Amino acids.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700002
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Elevated amniotic fluid amino acid levels in fetuses with gastroschisis BJMBR
Kale,A.; Kale,E.; Akdeniz,N.; Canoruc,N..
Our objective was to measure maternal plasma and amniotic fluid amino acid concentrations in pregnant women diagnosed as having fetuses with gastroschisis in the second trimester of pregnancy. Twenty-one pregnant women who had fetuses with gastroschisis detected by ultrasonography (gastroschisis group) in the second trimester and 32 women who had abnormal triple screenings indicating an increased risk for Down syndrome but had healthy fetuses (control group) were enrolled in the study. Amniotic fluid was obtained by amniocentesis, and maternal plasma samples were taken simultaneously. The chromosomal analysis of the study and control groups was normal. Levels of free amino acids and non-essential amino acids were measured in plasma and amniotic fluid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Gastroschisis; Amniotic fluid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800004
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Media components and amino acid supplements influencing the production of fruity aroma by Geotrichum candidum BJM
Pinotti,T.; Carvalho,P.M.B.; Garcia,K.M.G.; Silva,T.R.; Hagler,Allen Norton; Leite,S.G.F..
The ability of Geotrichum candidum to produce fruity aroma in food grade sucrose, molasses, corn steep liquor and peptone based culture media was tested by sensory evaluation and analyzed by gas chromatography mass spectrometry. A strong and sweet fruity aroma was produced from molasses, with peptone or corn steep liquor stimulating aroma production. Molasses with peptone supplemented with leucine, valine, or alanine yielded better fruity aroma production and the presence of many esters was consistent with the fruity aroma production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Geotrichum candidum; Aroma; Corn steep liquor; Molasses; Amino acids.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400017
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Effects of monosulfuron-ester on metabolic processes of nitrogen-fixing cyanobacteria Anabaena flos-aquae and Anabaena azotica BJM
Shen,Jian Ying; Liao,Jin Zhi; Guo,Li Li; Su,Rui Fang.
Abstract Presence of the relatively new sulfonylurea herbicide monosulfuron-ester at 0.03-300 nmol/L affected the growth of two non-target nitrogen-fixing cyanobacteria (Anabaena flos-aquae and Anabaena azotica) and substantially inhibited in vitro Acetolactate synthase activity, with IC50 of 3.3 and 101.3 nmol/L for A. flos-aquae and A. azotica, respectively. Presenting in 30-300 nmol/L, it inhibited protein synthesis of the cyanobacteria with less amino acids produced as its concentration increased. Our findings support the view that monosulfuron-ester toxicity in both nitrogen-fixing cyanobacteria is due to its interference with protein metabolism via inhibition of branch-chain amino acid biosynthesis, and particularly Acetolactate synthase activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetolactate synthase; Amino acids; Cyanobacteria; Monosulfuron-ester.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300544
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Isolation of enzymes involved in threonine biosynthesis from sorghum seeds Braz. J. Plant Physiol.
Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Meinhardt,Lyndel Wayne; Lea,Peter John; Azevedo,Ricardo Antunes.
Cereal seeds are poor in essential amino acids, particularly lysine, tryptophan and threonine. The amino acids lysine and threonine are synthesized in the aspartate pathway. Although most of the enzymes of the aspartate pathway have been isolated and characterized in higher plant species, the metabolism of lysine and threonine is totally unknown in sorghum. We have isolated two enzymes, aspartate kinase (AK) and homoserine dehydrogenase (HSDH) from sorghum. Optimum assay conditions were established for the determination of AK and HSDH activities. The highest level of activity was observed in immature seeds. AK was shown to be inhibited by threonine and lysine indicating the existence of at least two isoenzymes, one sensitive to threonine inhibition and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Aspartate kinase; Homoserine dehydrogenase; Lysine; Threonine.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000200005
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Lysine catabolism: flow, metabolic role and regulation Braz. J. Plant Physiol.
Fornazier,Ricardo Francisco; Azevedo,Ricardo Antunes; Ferreira,Renato Rodrigues; Varisi,Vanderlei Aparecido.
Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result of these processes. In the last 40 years, the enzymes involved in lysine biosynthesis have been purified and characterized from some plant species such as carrot, maize, barley, rice, and coix. Recent reports have revealed that lysine degradation might be related to various physiological processes, for instance growth, development and response to environmental...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Lysine; Metabolism; Mutants.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100002
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Amino acids and carbohydrates absorption by Na+-dependent transporters in the pyloric ceca of Hoplias malabaricus (Erythrinidae) Ciência Rural
Vieira,Vania Lucia Pimentel; Baldisserotto,Bernardo.
Information about amino acids and carbohydrate absorption in fish is important to formulate an adequate diet to obtain optimal growth. Therefore, the objective of this study was to investigate if Na+-dependent transporters are involved on the absorption of glycine, L-glutamine, L-leucine, L-lysine, L-proline, L-alanine, and the carbohydrates fructose and glucose in the pyloric ceca of Hoplias malabaricus. The pyloric ceca were mounted in a system of continuous perfusion "in vitro". Amino acids and carbohydrates were placed on the mucosal side at concentrations of 10, 20, and 40mM. The serosal side of the pyloric ceca was positive in relation to the mucosal side. The addition of glycine, L-glutamine, L-leucine, L-lysine, L-proline (all tested...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hoplias malabaricus; Amino acids; Carbohydrates; Intestinal absorption; Na+-dependent transporters; Pyloric ceca.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000500009
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Optimization of crude protein in diets for Nile tilapia reared in net pens: performance, hematology, and water quality Ciência Rural
Puppo,Débora Del; Haese,Douglas; Gomes,Levy de Carvalho; Kill,João Luís; Brugnara,Enrique Coelho; Barcellos,Joyce; Haddade,Ismail Ramalho; Ton,Nielton Cezar.
ABSTRACT: Two experiments were conducted to evaluate the effects of reducing dietary crude protein (CP), based on the ideal protein concept for Nile tilapia reared in net pens. The experimental (isocaloric, isocalcium, and isophosphoric) diets were formulated to contain 270, 300, 330, and 360g kg-1 CP. In experiment 1, 4320 Nile tilapia (13.5±0.82g) were used to evaluate the performance and hematological parameters. The experimental design was completely randomized and the fish were distributed in 24 net pens (1.0m3), with four diets, six replicates, and 180 fishes per experimental unit. In experiment 2, 40 Nile tilapia (22.5±0.56g) were used to evaluate the ammonia excretion. Fish were distributed in 40 aquaria (3.0L), with one fish per aquarium (n=10)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Performance; Statistic models; Oreochromis niloticus; Environmental pollution.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300651
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Digestible threonine-to-lysine ideal ratio for laying hens fed with low-protein diets from 24 to 40 weeks of age Ciência Rural
Pastore,Silvana Marques; Albino,Luiz Fernando Teixeira; Gomes,Paulo Cezar; Oliveira,Will Pereira de; Viana,Gabriel da Silva; Silva,Eliane Aparecida da; Alves,Warley Junior.
ABSTRACT: This trial was performed to determine the dietary digestible threonine-to-lysine (dig. Thr-to-Lys) ideal ratio for laying hens fed with low-protein diets from 24 to 40 weeks of age. At 24 weeks of age, two hundred forty Hy Line W-36 laying hens were randomly assigned to five treatments, represented by five dig Thr-to-Lys ratios (60; 67; 74; 81 and 88%), with eight replicates each one with six hens per experimental unit. Digestible Thr-to-Lys did not affect laying hen performance and egg quality, except for digestible Threonine intake, which showed linear increase as dig. Thr-to-Lys ratios increased. Linear broken-line model was chosen to analyze data according to lack of fit. According to linear broken-linen estimates, digestible Threonine ideal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Performance; Egg quality; Ratios among amino acids.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202229
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Phosphorylated protein concentrate pumpkin seed (Cucurbita moschata): optimization by response surface methodology and nutritional characterization Ciência Rural
Lovatto,Naglezi de Menezes; Loureiro,Bruno Bianchi; Bender,Ana Betine Beutinger; Loureiro,Cristiane Bianchi; Goulart,Fernanda Rodrigues; Speroni,Caroline Sefrin; Macagnan,Fernanda Teixeira; Piana,Mariana; Silva,Leila Picolli da.
ABSTRACT: Response surface methodology was used to determine optimum conditions for extraction of protein from pumpkin seed meal. A central composite rotational design (CCRD) including independent variables such, pH and sodium trimetaphosphate (STMP) content was used. Maximum yield was obtained at a central point of CCRD, with STMP content and pH of 4% and 4.5, respectively. These conditions resulted in protein yield of 50.04 g of soluble protein from extract/100 g pumpkin seed. The optimization of pH and STMP content allowed obtaining a product with a high protein concentration (62.56 g 100 g-1), digestibility (62.03 g 100 g-1) and concentration of essential amino acids (27.26 g 100 g-1). Regarding the polyphenols concentration, phosphorylated protein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Central composite rotational design; In vitro digestibility; New ingredient.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200751
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Top-dressing 1% arginine supplementation in the lactation diet of sows does not affect the litter performance and milk composition Ciência Rural
Dallanora,Djane; Walter,Marina Patricia; Marcon,Jéssica; Saremba,Camila; Bernardi,Mari Lourdes; Wentz,Ivo; Bortolozzo,Fernando Pandolfo.
ABSTRACT: The study aimed to evaluate the effects of arginine supplementation in the lactation diet of sows on their milk composition, litter performance and piglet survival. Sixty-four lactating Landrace x Large White sows, parity 1 to 7, were randomly assigned to two treatments: 1) Control - a corn/soybean meal based diet with 1.10% standardized ileal digestible (SID) lysine and 3,475kcal of metabolizable energy (ME) kg-1, and 2) arginine - the control diet top-dressed daily with arginine at 1% of feed allowance. The daily feed allowance per sow was 5.0 and 7.5kg from day (D)0 to D7 and D8 to D21, respectively. The average litter size was 12.8 piglets after cross-fostering. Litters were weighed on D1, D10, and D21 of lactation and pre-weaning mortality...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Piglets; Weight gain..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801460
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