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Registros recuperados: 131 | |
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Chang,Chen-Chin; Ng,Chang-Chai; Wang,Chung-Yi; Shyu,Yuan-Tay. |
Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulose degrading; Enzymes; Vegetable wastes. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300004 |
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SANTOS,Caroline Menicoze dos; RIBEIRO,Amanda da Silva; GARCIA,Adriana; POLLI,Andressa Domingos; POLONIO,Julio Cesar; AZEVEDO,João Lúcio; PAMPHILE,João Alencar. |
ABSTRACT Endophyte microorganisms have great biotechnological interest, with features applicable to different areas and are potentially useful in agriculture. The current study determines the biotechnological potential of endophytic fungi, isolated from leaves of Sapindus saponaria, to control phytopathogenic fungi and evaluate their enzyme production. Molecular taxonomy was performed by sequencing of the ITS1-5.8S-ITS2 ribosomal DNA region, identifying the genera Phomopsis, Sordariomycetes, Diaporthe, and Colletotrichum. In vitro antagonism against phytopathogens showed better results against Fusarium solani and provided inhibition indices between 41.8 % and 67.5 %. The endophytic strain SS81 (Diaporthe citri) presented the highest antagonism index... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological control; Endophytes; Enzymes; Microbial antagonisms; Molecular taxonomy. |
Ano: 2019 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000200322 |
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Pádua,Ana Patrícia Sousa Lopes de; Freire,Karla Torres Lins de Sousa; Oliveira,Thays Gabrielle Lins de; Silva,Leticia Francisca da; Araújo-Magalhães,Gianne Rizzuto; Agamez-Montalvo,Gualberto Segundo; Silva,Iolanda Ramalho da; Bezerra,Jadson Diogo Pereira; Souza-Motta,Cristina Maria de. |
ABSTRACT We studied the fungal endophyte community of the leaves of Myracrodruon urundeuva in Brazil along with their potential to produce L-asparaginase. In total, 187 endophytes were isolated. The colonization rate of leaf fragments from caatinga (10.41 %) was lower than those from brejo de altitude (39.58 %). Sequences of ITS rDNA from all endophytes revealed relationships with Ascomycota (Botryosphaeriales, Chaetothyriales, Diaporthales, Eurotiales, Glomerellales, Hypocreales, and Pleosporales) and Basidiomycota (Polyporales). The most frequent endophytes were members of the genus Diaporthe. Talaromyces was an indicator genus for caatinga while Phyllosticta, Diaporthe, and Colletotrichum were for brejo de altitude. The composition of endophytic fungi... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian ecosystems; Diaporthe; Endophytes; Enzymes; Fungal richness. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100039 |
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Nery, Marcela Carlota; Rocha, Adriana de Souza; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Fialho, Cintia Maria Teixeira; Nery, Fernanda Carlota. |
The seeds of a sesame crop contain approximately 50% oil content, one of the main reasons for their cultivation. However, information regarding methodologies for the assessment of quality seeds of this culture are scarce. Therefore, the objective of this study was to adapt the methods of accelerated ageing tests for sesame seeds and investigate changes in the behaviour of isoenzymes. The physiological characterization of the seeds and electrophoretic analysis of the isoenzymes superoxide dismutase, esterase, catalase, alcohol dehydrogenase, malate dehydrogenase and isocitrate lyase were also performed. For the accelerated ageing test, the seeds were subjected to the traditional method, with a saturated NaCl solution, at ageing times of 0, 24, 48, 72, and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymes; Oilseed; Vigour; Quality; Sesamum indicum.; Produção e tecnologia de sementes. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39449 |
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Silva, Leandro Moreira; Instituto Federal Minas Gerais - Campus Bambuí; Geraldo, Adriano; Instituto Federal Minas Gerais - Campus Bambuí; Vieira Filho, Javer Alves; Instituto Federal Minas Gerais - Campus Bambuí; Machado, Luiz Carlos; Instituto Federal Minas Gerais - Campus Bambuí; Brito, Jerônimo Ávito Gonçalves de; Universidade Federal do Recôncavo Baiano; Bertechini, Antonio Gilberto; Universidade Fderal de Lavras. |
O objetivo do estudo foi avaliar o efeito da associação de carboidrases e fitase em dietas de poedeiras. Foram utilizadas 300 poedeiras Isa Brown com 26 semanas, submetidas aos tratamentos: Controle Positivo: sem enzimas e valorização dos nutrientes; T2: valorização em 1,5 e 6% a EMA (kcal kg-1), do milho e o farelo de soja, respectivamente, e redução nos níveis nutricionais conforme a matriz nutricional para a fitase; T3: valorização da EMA do milho e o farelo de soja (idem T2) e redução nos níveis de cálcio e fósforo conforme matriz nutricional da fitase; T4: T2 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase; T5: T3 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase. Foi utilizado delineamento inteiramente casualizado, seis... |
Tipo: Pesquisa de campo |
Palavras-chave: 5.04.03.00-1 desempenho; Qualidade de ovos; Enzimas energia metabolizável; Fósforo disponível; Valorização de dietas performance; Egg quality; Enzymes; Metabolizable energy; Disponible phosphorus; Valorization of diets. |
Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12735 |
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Vieira Filho, Javer Alves; Geraldo, Adriano; Machado, Luiz Carlos; Brito, Jerônimo Ávito Golçalvez de; Bertechini, Antônio Gilberto; Murakami, Elise Saori Floriano. |
The experiment was conducted with the objective of evaluating the effect of protease enzyme supplementation on performance parameters and quality shell of laying hens. We used 240 Isa Brown commercial laying hens with 44 weeks of age. A completely randomized split-plot design (5 periods of 21 days) with four treatments and six replications (10 hens per replication) was used. The experimental diets were formulated according to the requirements of the breed. The following parameters were evaluated: egg production, feed intake, feed conversion, average egg weight, egg loss, specific gravity, percentage and shell thickness. After collection, the data were analyzed with SISVAR Statistical Package, and the means compared by SNK test at 5% probability. It was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laying hen farming; Enzymes; Grains; Nutrition. |
Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/22830 |
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Nolan, Pearl; Doyle, Evelyn; O'Kiley, Padraig. |
The aims of this study were to quantify the effects of fibrolytic enzyme additives applied to each of four consecutive cuts of unwilted grass at ensiling on ensilage characteristics and specific CH4 yields (SMY) per unit mass and per unit land area. In light of the importance of the primary growth yield, the effects of the timing of Cut 1 were also investigated. Furthermore, the mass-SMY and area-SMY effect of any effluent produced during ensilage was determined.At each of four cuts that comprised annual growth, samples from four replicate plots of Lolium perenne and of Phleum pratense were untreated (control) or were treated with either of two fibrolytic enzymes (ENZ 1 and ENZ 2) prior to ensiling for 120 days. The mass-SMY of silages and effluents were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grass yield; Silage; Fibre; Enzymes; Effluent; CH4 yield per unit land area. |
Ano: 2018 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4159 |
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SOBRAL,LAUREANA V.; MELO,KELLY N.; SOUZA,CLECIANA M.; SILVA,SÍLVIO F.; SILVA,GILVANIA L.R.; SILVA,ANDRESSA L.F.; WANDERLEY,KATHARINE A.A.; OLIVEIRA,IDJANE S.; CRUZ,ROBERTA. |
ABSTRACT To the fungal microbiota the UFPE and biotechnological potential enzymatic and antimicrobial production. Air conditioned environments were sampled using a passive sedimentation technique, the air I ratio and the presence of aflatoxigenic strains evaluated for ANVISA. Icelles were to determine the enzymatic activity of lipase, amylase and protease metabolic liquids to determine antimicrobial activity. Diversity was observed in all CAV environments, CFU/m3 ranged from 14 to 290 and I/E ratio from 0.1 to 1.5. The of the fungal genera were: Aspergillus (50%), Penicillium (21%), Talaromyces (14%), Curvularia and Paecilomyces (7% each). Aspergillus sydowii (Bainier & Sartory) Thom & Church presented enzymatic activity and the Talaromyces... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Air quality; Bacteria; Enzymes; Filamentous fungi; Refrigerated environments. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502327 |
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ALVES,CARLA; COSTA,EDUARDA; SOFIATTI,JESSICA R.; FORTE,CESAR T.; WINTER,FÁBIO L.; HOLZ,CINTHIA M.; KAIZER,ROSILENE R.; GALON,LEANDRO. |
ABSTRACT Most herbicides applied in crop field, stay in the soil for a period, affecting next crop or even the plants using as green manure. Nowadays, the use of herbicides grow to increase productivity, mainly in the grain producing region north of Rio Grande do Sul state. The objective of this study was to evaluate the effects of herbicides fomesafen and sulfentrazone on antioxidant system in Avena sativa1, Vicia sativa2, Raphanus sativus and Lupinus albus. The plants were exposed to varying concentrations of fomesafen3 (0, 0.125, 0.25 and 0.5 kg ha -1) and sulfentrazone (0, 0.3, 0.6 and 1.2 kg ha-1). For this, the activities of, ascorbat peroxidase, catalase and guaiacol enzymes were analyzed, and the levels of MDA were quantificated. Fomesafen and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Enzymes; Agricultural plants; PROTOX system. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401533 |
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ARAÚJO,FÁBIO V. DE; NETTO,MARCELLE C.M.; AZEVEDO,GUSTAVO P.; JAYME,MARCELLY M.A.; NUNES-CARVALHO,MONICA C.; SILVA,MARIANA M.; CARMO,FLÁVIA L. DO. |
ABSTRACT Marine sponges has been a large reservoir of microbial diversity, with the presence of many species specific populations as well as producing biologically active compounds, which has attracted great biotechnological interest. In order to verify the influence of the environment in the composition of the bacterial community present in marine sponges and biotechnological potential of bacteria isolated from these organisms, three species of sponges and the waters surrounding them were collected in different beaches of Rio de Janeiro, Brazil. The profile of the bacterial community present in sponges and water was obtained by PCR-DGGE technique and the biotechnological potential of the strains isolated by producing amylase, cellulase, protease and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Biosurfactants; Enzymes; Marine sponges; PCR-DGGE.. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602785 |
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CASELI,LUCIANO. |
ABSTRACT In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980’s, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Langmuir-Blodgett; Enzymes; Biosensors; Air-water interface. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200631 |
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TEIXEIRA,ANITA F.S.; SILVA,JACQUELINE S.; VILELA,LAÍZE A.F.; COSTA,PATRÍCIA F.; COSTA,ELAINE M. DA; GUIMARÃES,AMANDA A.; SANTOS,JESSÉ V. DOS; SILVA,SÉRGIO H.G.; CARNEIRO,MARCO AURÉLIO C.; MOREIRA,FATIMA M.S.. |
Abstract: Several microbiological indicators of soil quality present high sensitivity, but little is known about the influence of topographic factors on them. This work aimed to evaluate variability of biological indicators of soil quality across a hillslope under native forest and the influence of topographic factors on them. Four positions on a hillslope were evaluated. Activity of the enzymes β-glucosidase, acid phosphatase, urease and fluorescein diacetate (FDA) hydrolysis were determined, as well as basal and substrate-induced respiration, and density of microorganisms: total bacteria, total fungi, actinobacteria, phosphate solubilizers, ammonifiers, native rhizobia, free-living N2-fixing bacteria, spores of arbuscular mycorrhizal fungi and percentage... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological indicators; Digital elevation model; Enzymes; Microbial activity; Random forest. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700866 |
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Registros recuperados: 131 | |
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