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| Registros recuperados: 425 | |
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| Richini-Pereira,VB; Bosco,SMG; Theodoro,RC; Barrozo,L; Bagagli,E. |
| Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Road-killed animals; Fungal pathogens; Eco-epidemiology; PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011 |
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| Soares,M. J. V.; Moraes,J. R. E.; Roselino,A. M. F.. |
| In human and canine renal histological studies of visceral leishmaniasis (VL), the etiological agent is rarely found in situ. The objective of this study was to evaluate PCR in identifying the etiological agent in spleen, liver, lymph node, and kidneys of VL-seropositive dogs. Twenty-five symptomatic (case group) and 15 asymptomatic (control group) VL-seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Serologic diagnosis was made by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Animals were subjected to euthanasia and necropsy. Renal fragments were immersed in buffered formaldehyde solution. Spleen, liver, lymph node, and kidney samples were collected and frozen at -70ºC until DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dogs; Leishmania; PCR; Kidney. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400011 |
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| Rahman,Mafruza S; Sharma,RK; Borah,P; Chakraborty,A; Devi,Mandakini RK; Longjam,N. |
| Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding alpha (α) toxin. Of the 102... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; PCR; Α toxin; Cpa gene; Polymerase chain reaction. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100010 |
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| Oliveira,LRC; Picka,MCM; Nicolete,VC; Calvi,SA; Marcondes-Machado,J. |
| The aim of the present study was to investigate the presence of Trypanosoma cruzi in the heart, liver, lung, and kidneys, using hemoculture and PCR analysis, of mice infected with different parasite strains during the acute and chronic phases of infection. Parasitemia curves revealed strain-specific biological behaviors. For the Y and JLP strains, the acute phase of infection started at days six and ten post-infection, parasitemia peaked at days seven and 15 post-infection, the chronic phase started at days nine and 28 post-infection, and animals started dying at days 19 and 120 post-infection, respectively. When the two strains were compared, the JLP strain exhibited reduced and slower replication rates associated with a delayed peak of parasitism and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; PCR; Hemoculture; Organs; Distribution. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100005 |
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| Miyashiro,S; Baldassi,L; Nassar,AFC. |
| Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small intestine samples of bovines that have died suddenly, after manifesting or not enteric or neurological disorders. Two strains (8.7%) were identified as type E, two (8.7%) as type D and the remainder as type A (82.6%). Commercial toxoids available in Brazil have no label claims for efficacy against type E-associated enteritis; however, the present study shows the occurrence of this infection. Furthermore,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Iota toxin; Sudden death; PCR; Cattle. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300010 |
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| Ozkan,O; Ahmet,C; Zafer,K. |
| Mitochondrial DNA (mtDNA) has been widely employed in phylogeographic and phylogenetic studies. In the present study, the genetic identification of the scorpion Androctonus crassicauda (Olivier, 1807) was carried out by using the 16S mitochondrial gene, since this scorpion represents the most important species in Turkey regarding scorpionism and antivenom production. Two genetic groups were found according to the sequence analysis results, while five different loci at the nucleotide level presented genetic variation in the 16S region when compared to a known A. crassicauda sequence data (GenBank, AJ277598). Nucleotide variations found in the current work constitute the first descriptive report for A. crassicauda. Moreover, future studies may enlighten the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpion; Androctonus crassicauda; PCR; Genotype; Turkey. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400010 |
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| Venerando,R; Rasmussen,LT; de Labio,RW; Gatti,LL; Francisco,O; Viani,GA; Rivera,LML; Payão,SLM. |
| The risk of developing gastric cancer is believed to be related to differences among Helicobacter pylori strains and the inflammatory responses mediated by host genetic factors. H. pylori infection is acquired at an early age and in the absence of appropriate antibiotic therapy, it generally persists for life. Tp53 gene regulates the transcription of several cytokines and chemokines involved in innate immunity and its action may be influenced by the presence of different H. pylori strains. The present study aimed to detect H. pylori in pediatric patients, to access Tp53 polymorphism at codon 72 and to correlate such findings with age and histopathological results. Three hundred and forty-two patients were analyzed. DNA from their gastric biopsies was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Childhood; Tp53 polymorphims; PCR. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400004 |
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| Groenewald, M.; Daniel, H.-M.; Robert, V.; Poot, G.A.; Smith, M.Th.. |
| The type species of the genus Debaryomyces, Debaryomyces hansenii, is a highly heterogeneous species. It has been isolated from a large diversity of natural sources including fruit, air, water, soil, but most frequently from processed food products. The species delineation of this yeast species urgently needs clarification. The heterogeneity in taxonomic characteristics has resulted in the description of varieties linked to D. hansenii. The aim of this study was to re-examine and classify D. hansenii using a polyphasic approach. A total of 65 D. hansenii isolates were examined, 57 representing the variety hansenii and nine the variety fabryi. The selection of strains for DNA reassociation and phylogenetic analysis was based on polymerase chain reaction... |
| Tipo: Article / Letter to the editor |
Palavras-chave: ACT1; Debaryomyces fabryi; Debaryomyces hansenii; Debaryomyces subglobosus; DNA reassociation; Maximal growth temperature; PCR; Ingerprinting; Taxonomy. |
| Ano: 2008 |
URL: http://www.repository.naturalis.nl/record/531832 |
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| Barreto,Marliton R.; Guimaraes,Cláudia T.; Teixeira,Flávia F.; Paiva,Edilson; Valicente,Fernando H.. |
| The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 10³ to 10(8) polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insect pathology; Fall armyworm larvae; PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100010 |
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| Rezende,Helder R; Soares,Renata M; Cerutti Jr,Crispim; Alves,Isabel C; Natal,Delsio; Urbinatti,Paulo R; Yamasaki,Tasciane; Falqueto,Aloísio; Malafronte,Rosely dos S. |
| Autochthonous malaria cases in the state of Espírito Santo, Brazil, are distributed in mountainous regions surrounded by the Atlantic Forest. While some aspects of this disease are unclear, detection of possible vector species can help to elucidate epidemiological uncertainties. Entomological and natural infection studies were carried out using anophelines (Diptera: Culicidae) captured in the municipality of Santa Tereza, ES. Monthly captures were made from March 2004 to February 2006. CDC-CO2 traps were used from dusk (6:00 P.M.) to dawn (6:00 A.M.) to capture anophelines in the following habitats: near the houses, in open areas (at ground level) and inside, and at the margins of the forest (canopy and ground level). Shannon light traps were also used at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anopheles cruzii; Autochthonous malaria; Plasmodium; PCR; Ecology. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000200017 |
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| Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇. |
| A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting... |
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Palavras-chave: Babesia caballi; PCR; IFAT. |
| Ano: 1998 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281 |
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| Watanabe, Malaika; Oikawa, T; Hiraoka, Hiroko; Kaneko, N; Itamoto, Kazuhito; Mizuno, Tohru; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽. |
| Three beagle dogs were inoculated with mice spleen/liver homogenate infected with Ehrlichia species detected from Ixodes ovatus (EIO) and one dog was used as a control. All three infected dogs did not show clinical signs of disease except for mild pyrexia throughout the 41-day study period. Splenomegaly was observed from Day 7 post-inoculation (p.i.) in two of the dogs. Hematological and biochemical abnormalities included mild thrombocytopenia, hypoproteinaemia, hypoalbuminaemia and increased C-reactive protein values. One of the dogs' splenic aspirate sample was PCR-positive for Ehrlichia Day 7 p.i. and another dogs' blood and bone marrow aspirate sample was PCR-positive Day 41 p.i. Sequence analysis of the PCR products showed 100% homology with the... |
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Palavras-chave: CANINE MONOCYTIC EHRLICHIOSIS; YAMAGUCHI PREFECTURE; INFECTION; JAPAN; DIAGNOSIS; KINETICS; TICKS; PCR. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/65 |
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| Kamyingkird, K.; Yangtara, S.; Desquesnes, M.; Cao, S.; Adjou Moumouni, P.K.; Jittapalapong, S.; Nimsupan, S.; Terkawi, M.A.; Masatani, T.; Nishikawa, Y.; Igarashi, I.; Xuan, X.. |
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Palavras-chave: Babesia caballi; ELISA; IFAT; PCR; Theileria equi. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4092 |
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| 田川, 道人; 大橋, 英二; 本村, 絹代; 千葉, 史織; 堀内, 雅之; 三好, 雅史; 猪熊, 壽; 松本, 高太郎; TAGAWA, Michihito; OHASHI, Eiji; MOTOMURA, Kinuyo; CHIBA, Shiori; HORIUCHI, Noriyuki; MIYOSHI, Masahumi; INOKUMA, Hisashi; MATSUMOTO, Kotaro. |
| 10歳の雄の雑種犬が，3週間前から持続する発熱及び元気消失を主訴に帯広畜産大学動物医療センターを受診した．心雑音及び心エコー検査において僧帽弁の疣贅物が確認され，抗生物質等により加療したが，第7病日より心不全の悪化を認め，第10病日に肺水腫により斃死した．病理解剖では，軽度の心拡大及び僧帽弁における多中心性疣贅物が認められた．血液培養検査では，Staphylococcus haemolyticusが検出されたが，疣贅物を用いたPCR検査ではStreptococcus canisと100％一致する塩基配列が得られた．血液培養分離菌はコンタミネーションであった可能性が高く，本症例の心内膜炎はStreptococcus canisによるものと考えられた．血液培養結果と実際の心内膜炎起因菌が異なる場合があることを考慮する必要がある． |
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Palavras-chave: 心内膜炎; PCR; Streptococcus canis. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4232 |
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| Registros recuperados: 425 | |
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