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An intimate link between antimicrobial peptide sequence diversity and binding to essential components of bacterial membranes ArchiMer
Schmitt, Paulina; Rosa, Rafael D.; Destoumieux-garzon, Delphine.
Antimicrobial peptides and proteins (AMPs) are widespread in the living kingdom. They are key effectors of defense reactions and mediators of competitions between organisms. They are often cationic and amphiphilic, which favors their interactions with the anionic membranes of microorganisms. Several AMP families do not directly alter membrane integrity but rather target conserved components of the bacterial membranes in a process that provides them with potent and specific antimicrobial activities. Thus, lipopolysaccharides (LPS), lipoteichoic acids (LTA) or the peptidoglycan precursor Lipid II are targeted by a broad series of AMPs. Studying the functional diversity of immune effectors tells us about the essential residues involved in AMP mechanism of...
Tipo: Text Palavras-chave: Functional diversity; Defensin; Anti-lipopolysaccharide factor; Mechanism of action; Resistance.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00286/39710/38165.pdf
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Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome ArchiMer
De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Perez, Valérie; Stenger, Pierre-louis; Degremont, Lionel; Montagnani, Caroline; Escoubas, Jean Michel; Haffner, Philippe; Allienne, Jean-françois; Leroy, Marc; Lagarde, Franck; Vidal Dupiol, Jeremie; Gueguen, Yannick; Mitta, Guillaume.
Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein...
Tipo: Text Palavras-chave: Pacific oyster; Oyster disease; Resistance; OsHV-1; Antiviral molecular pathways; Invertebrate immunity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00602/71369/69811.pdf
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Interaction between Insects, Toxins, and Bacteria: Have We Been Wrong So Far? ArchiMer
Tetreau, Guillaume.
Toxins are a major virulence factor produced by many pathogenic bacteria. In vertebrates, the response of hosts to the bacteria is inseparable from the response to the toxins, allowing a comprehensive understanding of this tripartite host-pathogen-toxin interaction. However, in invertebrates, this interaction has been investigated by two complementary but historically distinct fields of research: toxinology and immunology. In this article, I highlight how such dichotomy between these two fields led to a biased, or even erroneous view of the ecology and evolution of the interaction between insects, toxins, and bacteria. I focus on the reason behind such a dichotomy, on how to bridge the fields together, and on confounding effects that could bias the outcome...
Tipo: Text Palavras-chave: Invertebrate immunity; Host-pathogens interaction; Toxins; Bacillus thuringiensis; Resistance.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00457/56834/78950.pdf
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Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone ArchiMer
Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf
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Entangled effects of allelic and clonal (genotypic) richness in the resistance and resilience of experimental populations of the seagrass Zostera noltii to diatom invasion ArchiMer
Massa, Sonia I.; Paulino, Cristina M.; Serrao, Ester A.; Duarte, Carlos M.; Arnaud-haond, Sophie.
Background: The relationship between species diversity and components of ecosystem stability has been extensively studied, whilst the influence of the genetic component of biodiversity remains poorly understood. Here we manipulated both genotypic and allelic richness of the seagrass Zostera noltii, in order to explore their respective influences on the resistance of the experimental population to stress. Thus far intra-specific diversity was seldom taken into account in management plans, and restoration actions showed very low success. Information is therefore needed to understand the factors affecting resistance and resilience of populations. Results: Our results show a positive influence of both allelic and genotypic richness on the resistance of...
Tipo: Text Palavras-chave: Allelic richness; Genotypic richness; Resistance; Resilience; Zostera noltii; Biotic stress; Seagrass.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00166/27748/25946.pdf
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Study on the susceptibility of Enterococcus faecalis from infectious processes to ciprofloxacin and vancomycin J. Venom. Anim. Toxins incl. Trop. Dis.
Genaro,A.; Cunha,M. L. R. S.; Lopes,C. A. M..
Enterococcus faecalis is considered a pathogen responsible for hospital infections and, due to its frequent multi-resistant profile, has caused preoccupations among many medical authorities. The objective of this study was to determine the antimicrobial susceptibility of 74 strains isolated from blood cultures and purulent secretions to vancomycin and ciprofloxacin through the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by using the Microdilution test. The results showed a greater efficacy of vancomycin compared to ciprofloxacin (98.6% of the strains were inhibited by vancomycin at lower concentrations: 0.06 - 1 µg/ml). However, in the MBC analysis 73% of the strains showed a MBC of vancomycin only at high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus faecalis; Vancomycin; Ciprofloxacin; Resistance; Spontaneous mutation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300004
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Influence of host resistance and insecticide seed treatments on curly top in sugar beets NWISRL
Strausbaugh, C.A.; Gillen, A.M..
Tipo: Article Palavras-chave: Resistance; Curly top; Insecticide.
Ano: 2006 URL: http://eprints.nwisrl.ars.usda.gov/41/1/1191.pdf
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Beet curly top resistance in USDA-ARS plant introduction lines, 2014. NWISRL
Strausbaugh, C.A.; Panella, Leonard W..
Curly top caused by Beet curly top virus (BCTV) is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. In order to identify novel sources of curly top resistance, twenty-four plant introduction (PI) lines were screened in a disease nursery in 2014. The lines were arranged in a randomized complete block design with three replications. A curly top epiphytotic was created by releasing approximately 6 viruliferous beet leafhoppers per plant at the four- to six-leaf growth stage on 23 Jun. Foliar symptoms were evaluated on 16 Jul using a scale of 0-9 (0 = healthy and 9 = dead) in a...
Tipo: Article Palavras-chave: Curly top; Resistance; Verticillium wilt.
Ano: 2015 URL: http://eprints.nwisrl.ars.usda.gov/1588/1/1546.pdf
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Evaluation of fungicide and biological treatments for control of fungal storage rots in sugar beet, 2014 NWISRL
Strausbaugh, C.A.; Olsen L, Nora; Frazier J, Mary; Wambolt, C.
Preventing sucrose losses in storage is important to the economic viability of the sugar beet industry. In an effort to establish additional measures for reducing sucrose losses in storage, ten fungicide and/or biological treatments were evaluated on sugar beet roots in a commercial sugar beet storage building for their ability to limit fungal growth on roots harvested 2 Oct. Six of the treatments were applied as a direct spray to roots, but two treatments were applied as a cold fog and two others were applied as a thermal fog. The treated eight-beet root samples were arranged in a randomized complete block design with 6 replications on top of the commercial sugar beet pile inside a storage building. Roots were evaluated for fungal growth, root rot,...
Tipo: Article Palavras-chave: Resistance; Storage; Sugarbeet.
Ano: 2015 URL: http://eprints.nwisrl.ars.usda.gov/1603/1/1561.pdf
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Sugar beet germplasm evaluated for rhizomania and storage rot resistance in Idaho, 2011. NWISRL
Strausbaugh, C.A.; Panella, Leonard W..
Rhizomania caused by the Beet necrotic yellow vein virus (BNYVV) is a worldwide problem that can lead to loss of tonnage and lower percent sucrose in the field. BNYVV can also reduce the storability of roots. To identify germplasm with resistance to these problems, 14 sugarbeet germplasm lines developed by the USDA-ARS Ft. Collins sugarbeet program were screened in a field experiment arranged in a randomized complete block design with 5 replications. During the growing season plants were evaluated for foliar rhizomania symptoms. At harvest on 29 September, roots were evaluated for rhizomania symptoms and then placed into an indoor commercial sugarbeet storage building in Paul, ID. Foliar symptoms ranged from 0% for one of the resistant checks to 99%...
Tipo: Article Palavras-chave: Resistance; Rhizomania; Root rots; Sugarbeet.
Ano: 2012 URL: http://eprints.nwisrl.ars.usda.gov/1466/1/1431.pdf
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Resistance to Beet curly top in USDA-ARS Ft. Collins germplasm, 2015 NWISRL
Panella, Leonard W.; Strausbaugh, C.A..
Fifty sugar beet (Beta vulgaris L.) germplasm lines produced by the USDA-ARS Ft. Collins sugar beet program and two commercial check cultivars [SV2012RR (susceptible) and HM PM90 (resistant)] were screened for resistance to Beet curly top virus (BCTV). The curly top evaluation was conducted at the USDA-ARS North Farm in Kimberly, ID, which had been in barley in 2014. The germplasm was planted (density of 142,560 seeds/A) on May 27. The plots were two rows 10 ft long with 22-in row spacing and arranged in a randomized complete block design with three replications. The fields were sprinkler irrigated, cultivated, and hand weeded as necessary. Plant populations were thinned on June 20 Plants were inoculated at the four- to six-leaf growth stage on June...
Tipo: Article Palavras-chave: Curly top; Resistance; Sugarbeet.
Ano: 2016 URL: http://eprints.nwisrl.ars.usda.gov/1617/1/1574.pdf
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Ft. Collins sugar beet germplasm evaluated for rhizomania and storage rot resistance in Idaho, 2015 NWISRL
Panella, Leonard W.; Strausbaugh, C.A..
Fifty-seven sugar beet (Beta vulgaris L.) lines from the USDA-ARS Ft. Collins sugar beet program and four check cultivars were screened for resistance to Beet necrotic yellow vein virus (BNYVV), the causal agent of rhizomania, and storage rot. The rhizomania evaluation was conducted at the USDA-ARS North Farm in Kimberly, ID. Seed of the sugar beet germplasm lines was planted on April 21. One-row plots, 10 ft long with 22-in row spacing between rows were arranged in a randomized complete block design with 4 replications. The trial relied on natural infection for rhizomania and storage rot development. The plots were rated for foliar symptom (percentage of plants with yellow, stunted, upright leaves) development on July 8 and 20. At harvest, roots in...
Tipo: Article Palavras-chave: Resistance; Rhizomania; Storage; Sugarbeet.
Ano: 2016 URL: http://eprints.nwisrl.ars.usda.gov/1628/1/1585.pdf
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Experimental sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2015 NWISRL
Strausbaugh, C.A.; Wambolt, C.
Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 32 commercial cultivars were screened by growing them in a sugar beet field infested with BNYVV in Kimberly, ID during the 2015 growing season in a randomized complete block design with 6 replications. At harvest on 28-29 September 2015, roots were dug and evaluated for symptoms of rhizomania and also placed in an indoor commercial sugar beet storage building. After 136 days in storage, samples were evaluated for surface rot, weight loss, and sucrose loss. Surface root rot ranged from 8 to 81%, weight loss ranged from 8.0 to 21.0%, sucrose losses...
Tipo: Article Palavras-chave: Resistance; Rhizomania; Storage; Sugarbeet.
Ano: 2016 URL: http://eprints.nwisrl.ars.usda.gov/1627/1/1584.pdf
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Registration of sugar beet doubled haploid line KDH13 with resistance to beet curly top NWISRL
Eujayl, Imad A.; Strausbaugh, C.A.; Chunsheng, Lu.
KDH13 is a sugar beet (Beta vulgaris L. ssp vulgaris) doubled haploid line (PI 663862) released as a genetic stock by USDA-ARS in cooperation with the Beet Sugar Development Foundation, Denver, CO. KDH13 is resistant to beet curly top (BCT) caused by Beet curly top virus which is transmitted by the beet leafhopper (Circulifer tenellus Baker). KDH13 was extracted from the BCT resistant C762-17 (PI 560130) parental line that segregates for BCT resistance and genetic male sterility. Intensive phenotypic screening was used for selecting plants from C762-17 that showed no symptoms under fully controlled infection conditions in the greenhouse. Seed from a single plant was used as a donor of floral buds for isolation of unfertilized ovaries to regenerate KDH13...
Tipo: Article Palavras-chave: Curly top; Genetics; Resistance; Sugarbeet.
Ano: 2016 URL: http://eprints.nwisrl.ars.usda.gov/1608/1/1566.pdf
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Experimental sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2014 NWISRL
Strausbaugh, C.A.; Eujayl, Imad A.; Wambolt, C.
Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 30 experimental cultivars were screened by growing them in a sugar beet field infested with BNYVV in Kimberly, ID during the 2014 growing season in a randomized complete block design with 6 replications. At harvest on 24-25 September 2014, roots were dug and evaluated for symptoms of rhizomania and also placed in an indoor commercial sugar beet storage building. After 138 days in storage, samples were evaluated for surface rot, weight loss, and sucrose loss. Surface root rot ranged from 6 to 82%, weight loss ranged from 9.1 to 17.5%, sucrose losses...
Tipo: Article Palavras-chave: Resistance; Rhizomania; Sugarbeet.
Ano: 2015 URL: http://eprints.nwisrl.ars.usda.gov/1601/1/1559.pdf
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Evaluation of experimental sugar beet hybrids for resistance to curly top in Malheur County, OR, 2005 NWISRL
Strausbaugh, C.A.; Gillen, A.M..
Twenty-five experimental sugarbeet hybrids submitted to the official variety trials were evaluated for resistance to Beet curly top virus (BCTV). Hybrids were planted in a commercial sprinkler-irrigated sugarbeet field and exposed to a natural epiphytotic of BCTV. Experimental units were arranged in a randomized complete block design with seven replications. Plots were evaluated for BCTV based on a Disease Index (DI) scale of 0 to 9 (no symptoms to dead) on Sept 14, 2004 by three raters. The moderately severe epiphytotic resulted in uniform disease pressure and significant differences between hybrids for DI, root yield, sugar content, and estimated recoverable sugar. Our disease ratings were positively correlated with (r = 0.632, P < 0.0001)...
Tipo: Article Palavras-chave: Resistance; Curly top.
Ano: 2006 URL: http://eprints.nwisrl.ars.usda.gov/1221/1/1193.pdf
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Evaluation of commercial sugarbeet hybrids for resistance to beet curly top in Canyon County, ID, 2004 NWISRL
Camp, S.; Foote, P.; Strausbaugh, C.A.; Gillen, A.M..
Tipo: Article Palavras-chave: Resistance; Curly top.
Ano: 2005 URL: http://eprints.nwisrl.ars.usda.gov/1214/1/1151.pdf
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Evaluation of experimental sugarbeet hybrids for resistance to beet curly top in Canyon County, ID, 2004 NWISRL
Strausbaugh, C.A.; Gillen, A.M.; Camp, S.; Foote, P..
Tipo: Article Palavras-chave: Resistance; Curly top.
Ano: 2005 URL: http://eprints.nwisrl.ars.usda.gov/1215/1/1152.pdf
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Evaluation of commercial sugar beet hybrids for resistance to curly top in Malheur County, OR, 2005 NWISRL
Strausbaugh, C.A.; Gillen, A.M..
Commercial sugar beet hybrids were evaluated for resistance to Beet severe curly top virus in a furrow-irrigated sugar beet field near Ontario, OR where curly top had been a problem in previous years. The field trial relied on natural infection and was planted on 6 Apr. Plots were planted to a density of 285,120 seeds/A, and thinned to 40,731 plants/A. Plots were four rows wide (22 in. between rows) and 23 ft long. The experimental design was a randomized complete block with eight replications. The crop was managed according to standard cultural practices. The weather during the growing season was normal except for above average temperatures in Mar and Aug and above average precipitation in Apr and May. Disease pressure was uniform and moderately...
Tipo: Article Palavras-chave: Resistance; Curly top.
Ano: 2006 URL: http://eprints.nwisrl.ars.usda.gov/1222/1/1194.pdf
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Beet curly top resistance in USDA-ARS Kimberly germplasm lines, 2015 NWISRL
Strausbaugh, C.A.; Eujayl, I.A..
Curly top caused by Beet curly top virus is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. In order to identify novel sources of curly top resistance, 14 sugar beet lines were screened in a disease nursery in 2015. The lines were arranged in a randomized complete block design with six replications. A curly top epiphytotic was created by releasing six viruliferous beet leafhoppers per plant at the four-to six-leaf growth stage on 24 Jun. Foliar symptoms were evaluated on 13 and 20 Jul using a scale of 0-9 (0 = healthy and 9 = dead) in a continuous manner. Curly top...
Tipo: Article Palavras-chave: Curly top; Resistance; Sugarbeet.
Ano: 2016 URL: http://eprints.nwisrl.ars.usda.gov/1616/1/1573.pdf
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