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| Registros recuperados: 112 | |
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| Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier; D'Amico, Florence; Rumebe, Myriam; Pichot, Yves; Le Gall, Patrik. |
| Created in 1992, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Ils activities are in keeping with two European Directives, 91167/EEC and 95170/EC and are a part of the institutional tasks of Ifremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to sm'vey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Granville bed and zone X). In 2005, only samples of the Granville bed could be analysed due to the difflculty to obtain flat oysters (sparse oyster beds, beds... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00086/19704/17346.pdf |
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| Randazzo, Walter; Khezri, Mohammad; Ollivier, Joanna; Le Guyader, Soizick; Rodriguez-diaz, Jesus; Aznar, Rosa; Sanchez, Gloria. |
| Shellfish contamination by human noroviruses (HuNoVs) is a serious health and economic problem. Recently an ISO procedure based on RT-qPCR for the quantitative detection of HuNoVs in shellfish has been issued, but these procedures cannot discriminate between inactivated and potentially infectious viruses. The aim of the present study was to optimize a pretreatment using PMAxx to better discriminate between intact and heat-treated HuNoVs in shellfish and sewage. To this end, the optimal conditions (30 min incubation with 100 μM of PMAxx and 0.5% of Triton, and double photoactivation) were applied to mussels, oysters and cockles artificially inoculated with thermally-inactivated (99 °C for 5 min) HuNoV GI and GII. This pretreatment reduced the signal of... |
| Tipo: Text |
Palavras-chave: Intercalating dyes; Viability PCR; Norovirus; Shellfish; Sewage; RT-qPCR. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00411/52208/52970.pdf |
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| Thebault, Anne; Arnich, Nathalie; Belin, Catherine; Neaud-masson, Nadine. |
| In France, the sampling strategy for the official monitoring of lipophilic phycotoxins in bivalve shellfish relies on the definition of risk areas and high risk periods, during which a systematic weekly analysis of toxins in shellfish is performed. Since 2010, high risk periods are defined as follows: the occurrence of one result above the European regulatory limit (160 μg equivalent okadaic acid/kg shellfish) over the last 3 years leads to that month being considered a high risk period. This definition was established according to a statistical analysis of the official monitoring results for the period 2003-2008, based on the mouse bioassay (MBA) as the official analytical method. As of the 1st January 2010, the MBA has been replaced by LC-MS/MS. In 2014,... |
| Tipo: Text |
Palavras-chave: Monitoring; Shellfish; Lipophilic phycotoxins; Bayesian inference. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00393/50470/51205.pdf |
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| Dubois, Eric; Merle, Ghislaine; Roquier, Catherine; Trompette, Aurélien; Le Guyader, Soizick; Cruciere, Catherine; Chomel, Jean-jacques. |
| Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5' untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the... |
| Tipo: Text |
Palavras-chave: Sequence analyze; RT PCR; Cell culture; Shellfish; Enterovirus. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-763.pdf |
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| Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Kopp, Joel; Mouillard, Gilbert; Gerla, Daniel; Le Saux, Jean-claude; Raguenes, Pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Fillon, Alain. |
| The REPAMO network (network ofmollusc pathology), created in 1986, ensures the survey of shellfish health status along French coasts according? to European Directives 91/67/EEC and 95/70/EEC. The aims of the network are 1 - to prevent the introduction and spread of exotic pathogens, 2-to study the means to decrease the impact of pathogens already present and to survey their evolution. In 2003, the REPAMO network focused on the survey oflisted diseases (bonamiosis and marteiliosis) in two areas under agreement process (Banc de Granville and zone X) and on the study of abnormal morlalities. Number of reported abnormal morlalities has increased in 2003. This rise can be partly explained by the recent network restructuring, but also by the summer heatwave and... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00086/19699/17340.pdf |
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| Maalouf, Haifa; Pommepuy, Monique; Le Guyader, Soizick. |
| Human fecal wastes contain a large variety of viruses that can enter the environment through discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. To explain bivalve mollusks contamination, several factors including human epidemiology, virus persistence through sewage treatment plant, and shellfish uptake may be suggested. Considering different outbreaks described in the literature, the most common route for transmission is accidental contamination after heavy rainfall, when extra loads cause an overflow, and release of untreated sewage into the... |
| Tipo: Text |
Palavras-chave: Shellfish; Outbreaks; Norovirus; Environmental conditions. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12252/9031.pdf |
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| Munschy, Catherine; Bely, Nadege; Pollono, Charles; Aminot, Yann. |
| Perfluoroalkyl substances (PFASs) were investigated in filter-feeding shellfish collected from 2013 to 2017 along the English Channel, Atlantic and Mediterranean coasts of France. PFOS (perfluorooctane sulfonate), PFTrDA (perfluorotridecanoic acid), PFTeDA (perfluorotetradecanoic acid), PFDoDA (perfluorododecanoic acid) and PFUnDA (perfluoroundecanoic acid) were detected in more than 80% of samples, thus indicating widespread contamination of the French coastal environment by these chemicals. The distribution of PFAS concentrations showed differences according to sampling locations and years. PFOS was the predominant PFAS in most samples collected from English Channel and Atlantic coasts until 2014, but the opposite was observed in 2015, 2016 and 2017,... |
| Tipo: Text |
Palavras-chave: PFOS; PFCAs; Shellfish; Levels; Profiles; Temporal trends. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60281/63706.pdf |
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| Baliere, Charlotte; Rince, Alain; Thevenot, Delphine; Gourmelon, Michele. |
| The presence of highly pathogenic Shiga-toxin-producing Escherichia coli (STEC) in shellfish, upstream waters and sediment from coastal shellfish sites was evaluated using the ISO/TS-13136 method. Shellfish (oysters, mussels and cockles), water and sediment samples were collected monthly over a period of 1 year. The method used real-time PCR detection of stx1, stx2 and eae genes and genetic markers corresponding to the five major serogroups (O157, O26, O103, O111 and O145) on enrichment broths and the identification of STEC when these genes and markers were detected. stx genes were detected in the broth of 33% of shellfish batches (n = 126), 91% of water samples (n = 117) and 28% of sediment (n = 39). One stx1+, eae+ O26:H11 strain was isolated from a... |
| Tipo: Text |
Palavras-chave: Eae; Sediment; Shellfish; Shiga-toxin-producing Escherichia coli; Stx; Water. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00250/36124/34975.pdf |
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| Abikhalil, Celina; Finkelstein, Darren S.; Conejero, Genevieve; Du Bois, Justin; Destoumieux-garzon, Delphine; Rolland, Jean-luc. |
| Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Harmful algae; Phytoplankton. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00391/50272/50905.pdf |
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| Amzil, Zouher; Sibat, Manoella; Royer, Florence; Savar, Veronique. |
| The French Phytoplankton and Phycotoxins monitoring network (REPHY) recently found positive or dubious negative shellfish samples using lipophilic toxins mouse bioassay. These samples were analyzed by liquid chromatography (LC) in combination with mass spectrometry (MS) to detect the following toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines (GYMs). Over the 2006-2007 period, chemical analyses revealed various lipophilic toxin profiles according to shellfish sampling locations. In addition to OA and/or PTX-2 and their derivatives, several other compounds were found for the first time in France: (1) during the summer of 2006, AZA-1 and AZA-2 in Queen... |
| Tipo: Text |
Palavras-chave: LC MS/MS analyses; Shellfish; Yessotoxin group; Azaspiracid group; Lipophilic toxins. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4560.pdf |
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| Parnaudeau, Sylvain; Le Mennec, Cecile; Miura, Takayuki; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Human enteric viruses discharged in raw sewage are hightly resistant and may be detected in surface waters, coastal areas and shellfish. Whereas noroviruses, responsible for relatively benign acute gastroentritis, are the most common agents implicated in shellfish borne outbreaks, other human viruses which cause severe human diseases, such as hepatitis A virus, may also be transmitted. Environmental studies have demonstrated that virus concentrations detected in shellfish are higher than in surrounding waters. This concentration may explain the high prevalence of noroviruses detected in various shellfish species collected in different countries. If many questions remain regarding viral contamination of shellfish, the most important seems to be the... |
| Tipo: Text |
Palavras-chave: Shellfish; Human enteric viruses; Norovirus; Hepatitis A virus; Coquillage; Virus entérique humain; Norovirus; Virus de l'hépatite A. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00317/42849/42258.pdf |
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| Mieszkin, Sophie; Caprais, Marie-paule; Le Mennec, Cecile; Le Goff, Manon; Edge, T. A.; Gourmelon, Michele. |
| Aims: The aim of this study was to identify the origin of faecal pollution impacting the Elorn estuary (Brittany, France) by applying microbial source tracking (MST) markers in both oysters and estuarine waters. Methods and Results: The MST markers used were as follows: (i) human-, ruminant- and pig-associated Bacteroidales markers by real-time PCR and (ii) human genogroup II and animal genogroup I of F-specific RNA bacteriophages (FRNAPH) by culture/genotyping and by direct real-time reverse-transcriptase PCR. The higher occurrence of the human genogroup II of F-specific RNA bacteriophages using a culture/genotyping method, and human-associated Bacteroidales marker by real-time PCR, allowed the identification of human faecal contamination as the... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Estuarine and river waters; F-specific RNA bacteriophages; Host-associated Bacteroidales markers; Microbial source tracking; Shellfish. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00153/26426/25250.pdf |
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| Le Guyader, Soizick; Pommepuy, Monique; Atmar, Robert. |
| Human and animal fecal wastes and urine contain a large number of different viruses that can enter the environment through the discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. Viruses are present in shellfish in very low numbers. Nevertheless, they are present in sufficient quantities to pose a health risk as presented. This low level of contamination has made it necessary to develop highly sensitive viral extraction methods to ensure virus recovery from shellfish tissues. The most common route for accidental contamination is after heavy... |
| Tipo: Text |
Palavras-chave: Human enteric viruses; Shellfish; Sewage; Persistence; Flux. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00015/12606/15279.pdf |
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| Registros recuperados: 112 | |
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