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| Registros recuperados: 97 | |
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| Barrueto Cid,L. Pedro; Carvalho,Luiz L.C.B. |
| The conventional technology for in vitro plant conservation for cassava (Manihot esculenta Crantz) germplasm collections is laborious due to the need for several sub-culturing procedures per year. This practice implies high costs for medium preparation, tissue culture tubes, time-consuming labor, risks of contamination, mislabeling of accession, and the need for large growth chambers. We have developed a new procedure using in vitro cultivated nodal axillary buds treated with different abscisic acid (ABA) concentrations to reduce the time for recycling transplants cultivated in a SP basic nutritive medium. Nodal explants were stored for three months with ABA. Plants were obtained after nodal axillary buds were placed in SP medium without ABA. Results... |
| Tipo: Journal article |
Palavras-chave: Tissue culture; Dormancy. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000300011 |
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| Souza,Rafaeli Aparecida Vieira de; Braga,Francyane Tavares; Setotaw,Tesfahun Alemu; Vieira Neto,João; Azevedo,Patrícia Helena de; Azevedo,Virgínia Helena de; Cançado,Geraldo Magela de Almeida. |
| The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Olea europaea; Culture medium; Complex mixture; Tissue culture; Zygotic embryos. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000200016 |
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| Pedroso,Rita Cássia Nascimento; Branquinho,Núbia Angélica de Ávila; Hara,Alessandra Cristina Boffino de Almeida Monteiro; Silva,Fabiano Guimarães; Kellner Filho,Luis Claudio; Silva,Marcio Luís Andrade; Cunha,Wilson Roberto; Pauletti,Patricia Mendonça; Januário,Ana Helena. |
| ABSTRACT: Hyptis marrubioides (Lamiaceae) is a medicinal plant that is native from Brazilian Cerrado. In vitro propagation techniques make use of elicitors to alter metabolic pathways, affecting how molecules are produced both qualitatively and quantitatively. This research aimed to evaluate how abiotic elicitors salicylic acid (SA) and silver nitrate (SN) at concentrations of 30µM or 60µM influence Hyptis marrubioides seedling growth by two different in vitro culture methods. The rutin content was quantified by HPLC-DAD. Compared to an untreated culture, the H. marrubioides methanolic extracts cultured in MS medium for 10 days followed by culture in MS medium containing SN (30µM) for 20 days had 1.28 times higher rutin content. In a second experiment,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salicylic acid; HPLC-DAD; Tissue culture; Silver nitrate; Elicitation; Plant growth regulators. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200400 |
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| Oliveira,Rayssa Camargo de; Asmar,Simone Abreu; Silva,Herick Fernando de Jesus; Morais,Tâmara Prado de; Luz,José Magno Queiroz. |
| ABSTRACT: Lavender is an aromatic ornamental plant that is used widely in the perfume, pharmaceutical, cosmetical and food industries. That is why it is important to study ways to promote a production of lavender raw material. For this, four experiments were carried out in order to study the germination and initial in vitro development of lavender. In the first part the efficiency of the use of hydrogen peroxide in the pre-treatment of seeds inoculated in culture media with different concentrations of gibberellic acid was evaluated. Besides that, the influence of the use of Growlux lamps and white fluorescent lamps on plantlets cultivated in MS and LS media at salts concentrations of 50 and 100% was evaluated. Finally, the effect of the gibberellic acid,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lavandula angustifolia; Tissue culture; GA3; Polyamines; Lamp. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100405 |
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| Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki. |
| ABSTRACT: The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum; Tissue culture; Growth regulators. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402 |
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| Silva,Luciano Coutinho; Paiva,Renato; Vargas,Daiane Peixoto; Silva,Diogo Pedrosa Corrêa da; Barbosa,Sandro; Herrera,Raírys Cravo. |
| Byrsonima intermedia A. Juss. é uma planta medicinal e frutífera do Cerrado, cuja propagação convencional é difícil, devido à presença do endocarpo extremamente lignificado. Embora o hipoclorito de sódio (NaOCl) seja amplamente utilizado para a desinfestação superficial, existem poucos relatos de seus efeitos sobre o crescimento do explante. Neste trabalho, o objetivo foi estudar o efeito de diferentes pHs e períodos de exposição de sementes de B. intermedia em uma solução de NaOCl. As sementes foram submetidas a diferentes tempos de exposição (1, 5 e 10 minutos) numa solução de NaOCl com diferentes pHs (5, 7, 10 e 12) e, após o tratamento com hipoclorito de sódio, os embriões foram inoculados em meio WPM com a concentração de 50% de sais sem sacarose,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Native plant; Tissue culture; PH; NaOCl.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400674 |
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| Lima,Ednabel Caracas; Paiva,Renato; Nogueira,Raírys Cravo; Soares,Fernanda Pereira; Emrich,Eduardo Bucsam; Silva,Álvaro Augusto Naves. |
| Croton urucurana Baill., a species belonging to the family Euphorbiaceae, can be useful in the recovery of degraded riparian areas. In the traditional medicine, it is widely used as cicatrizant and in the treatment of rheumatism. However, its seeds present dormancy and low viability, making the propagation of this species a challenge. With the objective of establishing an alternative route for the propagation, the effect of different concentrations of 2,4-D (2,4- dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) or TDZ (thidiazuron) and of NAA (1-naphthaleneacetic acid) with BAP and their possible combinations were evaluated for callus induction in leaf segments. Callus fresh mass was evaluated forty-five days after inoculation. The isolated use... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Calogenesis; Tissue culture. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100002 |
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| Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes. |
| With the objective to promote in vitro callus induction, cotyledon and hypocotyl segments of "perennial soybean" (Glycine wightii (Wight & Arn.) Verdc.) were inoculated in basal medium MS supplemented with sucrose (1.5 e 3%) and 0.8% agar and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin). The explants were maintained in a dark growth room at 28ºC. The best callus induction was observed in explants (cotyledon and hypocotyl) maintained in medium containing the combination of 2,4-D (1 mg.L-1), kinetin (0.1 mg.L-1) and 3% sucrose. To promote callus subculture, the MS medium was supplemented with different combinations of 2,4-D (0.5 to 4.0 mg.L-1), with or without kinetin (0.1 mg.L-1) and sucrose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine wightii; Leguminosae; Callogenesis; Tissue culture. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000600011 |
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| Lima-Brito,Alone; Resende,Sheila Vitória; Lima,Carolina Oliveira de Cerqueira; Alvim,Bruno Matos; Carneiro,Claudia Elena; Santana,José Raniere Ferreira de. |
| Syngonanthus mucugensis Giul. subsp. mucugensis is an herbaceous plant with significant economic value in the ornamental dry flower business. The restricted occurrence of the municipality Mucugê-BA, Brazil, exclusively associated with extractive exploitation, has considered this species as endangered. The objective of this work was to evaluate the organogenic potential of three different types of S. mucugensis subsp. mucugensis explants to promote the development of an alternative method to the propagation of the genetic resources of this important plant. The morphogenetic capacities of the leaf, stem and root this species was tested using Murashige and Skoog culture medium at half salt concentration and different concentrations of growth of regulators... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Organogenesis; Micropropagation; Straw-flowers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300010 |
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| Sant’Ana,Cecília Ramos de Oliveira; Paiva,Renato; Reis,Michele Valquíria dos; Silva,Diogo Pedrosa Corrêa da; Silva,Luciano Coutinho. |
| ABSTRACT The Campomanesia rufa is a fruitful species native from Brazil considered as endangered by the IUC (International Union for Conservation of Nature) and low information regarding its propagation is available. In this context, the aim of the present study was to develop in vitro germination, micropropagation and callogenesis protocols for the species. For in vitro germination, seeds were inoculated in MS medium supplemented with GA3 (gibberellic acid) and for shoot induction, the medium was supplemented with three different cytokinins BA (benzyladenine), BAP (6-Benzylaminopurine) or TDZ (Thidiazuron). For shoot growth, culture medium containing BA, BAP, TDZ were maintained under white fluorescent lamps and blue:red light-emitting diodes (LED). GA3... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Micropropagation; Myrtaceae; Casaqueira.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000400372 |
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| Sá,Jucieny Ferreira de; Sampaio,Emília dos Santos; Mendes,Maria Inês de Souza; Santos,Karen Cristina Fialho dos; Sousa,Antônio da Silva; Ledo,Carlos Alberto da Silva. |
| ABSTRACT The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cassava; Wild parental; Nutritional media; Tissue culture; Micropropagation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600598 |
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| Sá,Aline de Jesus; Lédo,Ana da Silva; Lédo,Carlos Alberto da Silva; Pasqual,Moacir; Silva,Ana Veruska Cruz da; Silva Junior,Josué Francisco da. |
| In micropropagation, especially for mangaba tree botanical variety of Northeastern Brazil, limiting aspects such as ethylene accumulation in the cultivation flask and loss of vigor in subcultures have been observed. This study was aimed at assessing the technical and scientific knowledge of the in vitro propagation of botanical mangaba tree variety and at improving the micropropagation protocol, establishing the in vitro cultivation time, the best type of flask sealing and explant at different micropropagation stages. For the establishment phase and for the first and second subcultures, the MS medium with 3% sucrose and 0.6% agar, supplemented with 1 mg L-1 IAA and 1 mg L-1 BA was used. Evaluations were performed at 30, 50 and 65 days of in vitro... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hancornia speciosa; Tissue culture; In vitro ethylene; Nodal segments. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400004 |
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| Pio,Leila Aparecida Salles; Ramos,José Darlan; Pasqual,Moacir; Santos,Flavia Carvalho; Junqueira,Keize Pereira. |
| The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus sinensis; Palinology; Tissue culture. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016 |
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| Registros recuperados: 97 | |
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