SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Benjamin,Jordan Max; Chippaux,Jean-Philippe; Sambo,Bio Tamou; Massougbodji,Achille.

Abstract Background The whole blood clotting test (WBCT) is a simple test of coagulation that is often used in the assessment, diagnosis, and therapeutic monitoring of snakebite patients in sub-Saharan Africa. WBCT requires only a clean glass tube and several milliliters of venous blood and is ideal for use in poorly equipped health centers throughout the rural areas where 95% of snakebites occur. However, questions surrounding the accuracy and reliability of the test remain unanswered due to variations in testing conditions and a lack of comparative research with which to validate them. This is the first study to evaluate WBCT results at both 20-min (WBCT20) and 30-min (WBCT30) reading times in the same group of snakebite patients. Methods In order to...

Effects of the European hornet ( Vespa crabro Linnaeus 1761) crude venom on its own species

Palavras-chave: Africa; Snakebite; Echis; Envenomation; Whole blood clotting test; WBCT; Venom-induced consumption coagulopathy; Carpet viper; Saw-scaled viper.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100308

Efficacy of sertraline against Trypanosoma cruzi: an in vitro and in silico study

Nadolski,Jerzy.

Background Lethal dose 50% is a classical index of toxicity that usually employs small rodents as experimental animals. Therefore, scarce data are available on the effects of venom on invertebrates, particularly the impact of wasp venom on its own species. Findings In the present study, the lethality of Vespa crabro venom on its own species was studied. Lethal dose 50% values of crude venom on workers of hornet Vespa crabro were estimated to be 4.0 mg/kg of body weight. Conclusions Wasps can use their venom apparatus effectively when attacking foreign workers that appear in the immediate vicinity of their nest. The toxins released during stinging are potent enough to kill. The result of this study eliminates the popular myth that venomous animals can be...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: European hornet; Vespa crabro; Hornet venoms; LD50.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100401

Ferreira,Daiane Dias; Mesquita,Juliana Tonini; Silva,Thais Alves da Costa; Romanelli,Maiara Maria; Batista,Denise da Gama Jaen; Silva,Cristiane França da; Gama,Aline Nefertiti Silva da; Neves,Bruno Junior; Melo-Filho,Cleber Camilo; Soeiro,Maria de Nazare Correia; Andrade,Carolina Horta; Tempone,Andre Gustavo.

Abstract Background: Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. Methods: In this work, we studied the activity of the antidepressant drug sertraline against Trypanosoma cruzi trypomastigotes and intracellular amastigotes of the Y and Tulahuen strains, and investigated its action mode using cell biology and in silico approaches. Results: Sertraline demonstrated in vitro efficacy against intracellular amastigotes of both T. cruzi strains inside different host cells, including cardiomyocytes, with IC50 values between 1 to 10 μM, and activity against bloodstream trypomastigotes, with IC50 of 14 μM. Considering the mammalian cytotoxicity, the drug resulted in a selectivity...

Cathepsin B/X is secreted by Echinometra lucunter sea urchin spines, a structure rich in granular cells and toxins

Palavras-chave: Trypanosoma cruzi; Drug; Treatment; Sertraline; Drug repurposing; Drug repositioning.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100321

Sciani,Juliana Mozer; Antoniazzi,Marta Maria; Neves,Adriana da Costa; Pimenta,Daniel Carvalho.

Background Echinometra lucunter is a common American sea urchin responsible for the majority of the marine accidents in Brazil. Although not lethal, these accidents are reported to be extremely painful. Recently, our group described the presence of toxins in its spines that contribute to the pathological reactions. Additionally, we have observed that the E. lucunter spines can regenerate when broken. In the present work we evaluated the enzymatic activities of sea urchin spine extracts in order to identify an enzyme that could contribute not only to the toxicity, but also participate in the spine growth and regeneration. Results The spine aqueous extract was tested for peptidase activity, with synthetic substrates, in the presence and absence of inhibitors...

The importance of serological assays in diagnosing acute pulmonary histoplasmosis

Palavras-chave: Echinometra lucunter; Spines; Cathepsin; Proteolysis.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100318

Freitas,RS; Carvalho-Vivi,JO; Zamboni,IM; Assis,CM; Costa-Martins,JE; Vicentini-Moreira,AP.

Histoplasmosis is a systemic mycosis caused by inhalation of Histoplasma capsulatum microconidia. The disease does not normally affect immunocompetent individuals after a single, transient inhalation exposure. However, longer exposure may cause chronic or disseminated acute pulmonary infection. Herein, we report the case of a 24-year-old immunocompetent patient, who presented fever, cough and dyspnea for one month. The chest radiography revealed interstitial infiltrate and diffuse micronodules. The patient reported having had close and prolonged contact with bats. Diagnosis was confirmed by positive double immunodifusion and immunoblotting assays. She was treated with ketoconazole (400 mg) and there was complete resolution of the disease.

Viper bites complicate chronic agrochemical nephropathy in rural Sri Lanka

Palavras-chave: Histoplasmosis; Histoplasma capsulatum; Immunocompetence; Serodiagnosis; Bats.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200010

Silva,Anjana; Samarasinghe,Rivikelum; Pilapitiya,Senaka; Dahanayake,Niroshana; Siribaddana,Sisira.

Snakebite is a common occupational health hazard among Sri Lankan agricultural workers, particularly in the North Central Province. Viperine snakes, mainly Russell’s viper envenomation, frequently lead to acute renal failure. During the last two decades, an agrochemical nephropathy, a chronic tubulointerstitial disease has rapidly spread over this area leading to high morbidity and mortality. Most of the epidemiological characteristics of these two conditions overlap, increasing the chances of co-occurrence. Herein, we describe four representative cases of viperine snakebites leading to variable clinical presentations, in patients with chronic agrochemical nephropathy, including two patients presented with acute and delayed anuria. These cases suggest the...

Bacterial contamination of the catfish (Cathorops agassizii - Valenciennes, 1839 and Genidens genidens - Agassiz, 1829) sting

Palavras-chave: Snakebite; Chronic kidney disease; Agricultural nephropathy; Epidemiology; Anuria.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200505

Junqueira,Marcos Emerson Pinheiro.

Hepatitis B virus: evaluation of infection risk and serological response to vaccine in hospital cleaners of the Botucatu School of Medicine

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300015

Antibacterial activity of propolis collected in different regions of Brazil

Osti,C..

Tipo: Info:eu-repo/semantics/other

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200010

Gonsales,G. Z.; Orsi,R. O.; Fernandes Júnior,A.; Rodrigues,P.; Funari,S. R. C..

The aim of this study was to investigate the antibacterial activity of propolis samples from Goiás, Paraná and São Paulo States, Brazil, and their flavonoids content. Ethanolic extracts of propolis (EEP) were prepared (30g of propolis in 70% ethanol), and the microorganisms Staphylococcus aureus and Escherichia coli were tested. The methodology employed was agar diffusion using filter paper discs. Ampicillin and tetracycline were used as controls. Antibacterial activity was determined by the reading of inhibition zone diameters (mm) after 24 hours incubation at 37°C. Results demonstrated that EEP inhibited the growth of Staphylococcus aureus but not that of Escherichia coli. Tetracycline and ampicillin showed an efficient action against both bacteria....

Identification and antimicrobial susceptibility of Staphylococcus from home-treated peritoneal dialysis patients

Palavras-chave: Propolis; Antibacterial activity; Flavonoids.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200009

Batalha,JEN; Cunha,MLRS; Montelli,AC; Barreti,P; Caramori,JCT.

Staphylococcus aureus is the main agent of infections during peritoneal dialysis (PD). The presence of S. aureus in the nasal cavity has been extensively studied and suggested as a risk factor of dialysis-related infections, whereas coagulase-negative Staphylococcus (CNS) species are frequently considered part of the normal human microbiota. The aim of this study was to identify Staphylococcus in the nasal cavity, pericatheter skin and peritoneal effluent from PD patients, as well as to evaluate the antimicrobial activity evolution in vitro. Thirty-two chronic PD patients were observed during 12 months and had nasal and pericatheter skin samples collected for culture. When peritonitis was detected, samples were also collected from the peritoneal effluent...

Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India

Palavras-chave: Peritoneal dialysis (PD); Peritonitis; Risk factor; Staphylococcus; Coagulase-negative Staphylococcus (CNS).

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200005

Thangaraj,S; Bragadeeswaran,S.

Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12% gel. In the hemolytic assay, both extracts exhibited hemolytic effect on...

Stings from Euscorpius flavicaudis (De Geer, 1778) (Scorpiones, Euscorpiidae) during pregnancy: a clinical case report

Palavras-chave: Aqueous extract; Neurotoxicity; Mouse bioassay; Analgesic activity.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100007

Dutto,M; Dutto,L; Bertero,M; Goyffon,M.

We report a case in which a 21-week pregnant woman was stung by a Euscorpius flavicaudis (De Geer, 1778) scorpion. Symptoms and signs experienced by the patient were the same as those documented in the literature and with no ill-effects for the pregnancy. Envenoming was local and of low degree of intensity. It is important to emphasize that the patient was stung in her home, which differs from stings in most other parts of the world, in which scorpionism is mostly a risk in outdoor areas.

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

Palavras-chave: Euscorpius flavicaudis; Sting; Scorpionism; Pregnant woman; Clinical; Symptoms.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400018

Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B..

ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom....

Leptospira spp. infection in sheep herds in southeast Brazil

Palavras-chave: Hyperimmunization; Irradiation; Crotalus durissus terrificus; ELISA; Serum neutralization; Detoxification.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300008

Barbante,Priscila; Shimabukuro,Fabio H; Langoni,Helio; Richini-Pereira,Virgínia B; Lucheis,Simone B.

Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys...

Snakebite in Bobo-Dioulasso, Burkina Faso: illustration of realities and challenges for care based on a clinical case

Palavras-chave: Leptospirosis; Ovine; Serology; Culture; PCR.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325

Kyelem,CG; Yaméogo,TM; Ouédraogo,SM; Zoungrana,J; Poda,GEA; Rouamba,MM; Ouangré,A; Kissou,SA; Rouamba,A.

We report herein the case of 19-year-old female farmer who suffered a double snakebite on the right foot. After an unsuccessful traditional treatment, she consulted a health center, 48 hours after the bite. Upon arrival at the hospital, she showed signs of severe damage, including hemorrhagic syndrome, extensive gangrene of the bitten limb and severe acute renal failure. Due to financial constraints, neither antivenom nor the scheduled amputation was performed. After 35 days of hospitalization, she returned home, against the advice of medical personnel. Our case summarizes the daily challenges of patients and practitioners that suffer snakebite envenomation in Bobo-Dioulasso, western Burkina Faso.

Palavras-chave: Envenomation; Snakebite; Management; Burkina Faso; Sub-Saharan Africa.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400021

An idea comes true...

Barraviera,Silvia Regina C. Sartori; Barraviera,Benedito.

Survival of a newborn from a pregnant woman with rabies infection

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200001

Zhen-Yu,Qu; Guo-Wei,Li; Qiao-Ge,Chen; Peng,Jiang; Chang,Liu; Alfred,Lam.

Abstract Background Rabies is very common in People’s Republic of China. Each year thousands of people die because of this disease, but rabies diagnosed in pregnancy is very rare. Case Presentation In this study, we report the case of a pregnant woman who was infected with the rabies virus after a dog bite. The symptoms of rabies appeared in labor and she died after pregnancy. Her baby and husband did not develop the disease. Conclusion The phenomenon that the newborn infant was healthy may be related to the protective role of placenta in resisting the invasion of the rabies virus or the absence of systemic viremia. The prompt administration of vaccines and anti-rabies immunoglobulin to the infant may have also contributed to his survival.

New reports on parasitism by Haplometroides buccicola (Digenea, Plagiorchiidae) in Brazilian snakes

Palavras-chave: Rabies; Pregnant; Newborn baby.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100502

Santos,KR; Barrella,TH; Zica,EOP; Silva,RJ.

The occurrence of Haplometroides buccicola (Digenea, Plagiorchiidae) in the esophagus of two Brazilian snakes is reported in the present study. The trematodes were collected from one Micrurus corallinus (Elapidae) and one Phalotris lativittatus (Colubridae); both snakes were found in Botucatu city, São Paulo State, Brazil. Morphological and morphometric analyses of the trematodes are presented. For the first time Micrurus corallinus has been recorded as a host for H. buccicola and this is the second time that P. lativittatus has been reported as a host for this trematode species.

Epidemiology of bee stings in Campina Grande, Paraíba state, Northeastern Brazil

Palavras-chave: Haplometroides buccicola; Plagiorchiidae; Phalotris lativittatus; Micrurus corallinus; Snakes.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300011

Linard,Ana Thaise Sousa; Barros,Rafaella Moreno; Sousa,Jorge Alves; Leite,Renner Souza.

Background : The present study aims to investigate the clinical-epidemiological characteristics of bee sting cases recorded between 2007 and 2012 in the city of Campina Grande, Paraiba state, Brazil. Data were collected from the database of the Injury Notification Information System of the Brazilian Ministry of Health.Results : A total of 459 bee sting cases were retrospectively analyzed. The average annual incidence was 19 cases per 100,000 inhabitants. Cases were distributed in all months of the year, with higher prevalence in September and February. Most victims were men aged between 20 and 29 years. The highest incidence of cases was recorded in urban areas. Victims were stung mainly on the head and torso and received medical assistance predominantly 1...