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| Registros recuperados: 131 | |
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| Melo,J.F.B.; Lundstedt,L. M; Inoue,L.A.K.; Metón,I.; Baanante,I.V.; Moraes,G.. |
| ABSTRACT The activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolism; Enzymes; Nutrition; Adaptation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501251 |
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| Teixeira-Neto,A.R.; Ferraz,G. C.; Moscardini,A. R. C.; Balsamão,G. M.; Souza,J. C. F.; Queiroz-Neto,A.. |
| This study tried to monitor the alterations of muscle enzymes activity - creatinokinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) - in a group of horses that participated of 70 and 100km distance rides in five competitions of an annual endurance championship under tropical climate. Pre ride levels (U/l) were 245.13±9.84 for CK, 496.61±14.76 for LDH, and 328.95±8.65 for AST. When compared to these levels, the results revealed a significant decrease in all enzymes activities in the first moment of the rides. Peak levels, significantly different, were reached, immediately after rides by CK (413.591±50.75); 24 hours post rides by LDH (628.61±33.30); and 48 hours after rides by AST (389.89±16.96). Monitoring of recovery period... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse; Endurance; Enzymes; Muscle. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300004 |
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| Coutinho,S.D.A.. |
| The relationship between production of enzymes and pathogenicity of Malassezia pachydermatis strains was studied. Thirty strains of M. pachydermatis isolated from dogs were used: 15 originating from animals with external otitis and 15 obtained from ears of asymptomatic dogs. The samples, obtained by introduction of a sterile swab into the auditory canal after cleaning the auricle with alcohol-ether solution, were seeded onto Sabouraud dextrose agar with chloramphenicol (100mg/l) and were incubated at 37°C for up to two weeks. The colonies were identified according to their macro-and-micromorphology. All strains were investigated for the production of chondroitin-sulphatase, hyaluronidase, phospholipase and proteinase. Most of the strains showed a high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Malassezia pachydermatis; Enzymes; Otitis; Microbiota. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003 |
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| Oliveira,M.C.; Rodrigues,E.A.; Marques,R.H.; Gravena,R.A.; Guandolini,G.C.; Moraes,V.M.B.. |
| The performance and the morphology of intestinal mucosa of broilers fed mannan-oligosaccharides (MOS) and enzymes (E) from one to 21-day-old were evaluated using 750 one-day-old chicks, assigned to a 2 x 2 + 1 factorial design - two levels of MOS (0 and 0.1%), two levels of E (0 and 0.05%) plus an antibiotic positive control diet - performing five treatments of five replications each one. MOS x E interaction was significant for both duodenal (P<0.002 and P<0.002) and ileal (P<0.04 and P<0.05) perimeters and heights of villi, being the values lower in the mucosa of birds fed non-supplemented diets. MOS based-diet determined an increase on perimeter of jejunal villi (P<0.05). Compared with antibiotic treatment group, villi perimeter... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Nutrition; Enzymes; Prebiotic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200025 |
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| Yan,Xu; Souza,Maria Aparecida; Pontes,Marcela Z. R.; Vitolo,Michele; Pessoa Júnior,Adalberto. |
| From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Affinity partitioning; Aqueous two-phase systems; Reactive dyes; Ligands; Enzymes. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030 |
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| Regina,Magali; Broetto,Fernando; Giovannozzi-Sermanni,Giovanni; Marabotini,Rosita; Peranni,Claudio. |
| This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25º C and in a bioreactor with four complete rotations daily at 25º C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shiitake; Enzymes; Agricultural residues. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200001 |
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| Tripathi,G; Kachhwaha,N.; Dabi,I.; Bandooni,N.. |
| The effects of varying temperatures (12 - 44° C) on the specific activity of cytoplasmic malate dehydrogenase ((cMDH), mitochondrial malate dehydrogenase (mMDH) and lactate dehydrogenase (LDH) of some earthworms (Metaphire posthuma, Perionyx sansibaricus and Lampito mauritii) were studied. The effects of different temperatures on supernatant and mitochondrial protein contents were also investigated. The specific activities of cMDH, mMDH and LDH of the earthworms decreased gradually as a function of increasing temperature from 12 to 44°C. Higher metabolic energy was needed to maintain the activity at low temperatures. Hence, the earthworms showed increased enzyme specific activity at low temperatures. However, the protein content increased upto 28°C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymes; Proteins; Earthworms; Temperatures. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400017 |
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| Petretski,J.H.; Kanashiro,M.; Silva,C.P.; Alves,E.W.; Kipnis,T.L.. |
| This article describes the presence of two new forms of a thrombin-like enzyme, both with apparent molecular masses of 38 kDa, in Bothrops atrox venom. Both share the ability to cleave fibrinogen into fibrin and to digest casein. Both present identical Km on the substrate BApNA. Their N-terminal amino acid sequences are identical for 26 residues, sharing 80% homology with batroxobin and flavoxobin. Two groups of monoclonal antibodies (mAbs) raised against the purified enzyme forms recognized different epitopes of the putative corresponding enzymes present in B. atrox crude venom. On Western blotting analysis of B. atrox crude venom, mAbs 5DB2C8, 5AA10 and 5CF11, but not mAbs 6CC5 and 6AD2-G5, revealed two or more protein bands ranging from 25 to 38 kDa. By... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venoms; Bothrops atrox; Enzymes; Thrombin-like enzymes; Blood coagulation system. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100005 |
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| Kumar,Vijay; Bharti,Alpana; Negi,Yogesh Kumar; Gusain,Omprakash; Pandey,Piyush; Bisht,Gajraj Singh. |
| Actinomycetes from earthworm castings were isolated and screened for their antimicrobial activity and industrial enzymes. A total of 48 isolates were obtained from 12 samples of earthworm castings. Highest numbers of isolates were recovered from forest site (58.33 %) as compared to grassland (25%) and agricultural land (16.66%). The growth patterns, mycelial coloration of abundance actinomycetes were documented. The dominant genera Identified by cultural, morphological and physiological characteristics were Streptomyces (60.41%) followed by Streptosporangium (10.41%), Saccharopolyspora (6.25%) and Nocardia (6.25%). Besides these, other genera like Micromonospora, Actinomadura, Microbispora, Planobispora and Nocardiopsis were also recovered but in low... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial activity; Enzymes; Earthworm castings; Actinomycetes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100022 |
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| Itako,Adriana Terumi; Tolentino Júnior,João Batista; Silva Júnior,Tadeu Antônio Fernandes da; Soman,José Marcelo; Maringoni,Antonio Carlos. |
| The bacterial spot of tomato, caused by Xanthomonas spp., is a very important disease, especially in the hot and humid periods of the year. The chemical control of the disease has not been very effective for a number of reasons. This study aimed to evaluate, under greenhouse conditions, the efficacy of leaf-spraying chemicals (acibenzolar-S-methyl (ASM) (0.025 g.L−1), fluazinam (0.25 g.L−1), pyraclostrobin (0.08 g.L−1), pyraclostrobin + methiran (0.02 g.L−1 + 2.2 g.L−1), copper oxychloride (1.50 g.L−1), mancozeb + copper oxychloride (0.88 g.L−1 + 0.60 g.L−1), and oxytetracycline (0.40 g.L−1)) on control of bacterial spot. Tomatoes Santa Clara and Gisele cultivars were pulverized 3 days before inoculation with Xanthomonas perforans. The production of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum lycopersicum; Bacterial spot; Control; Enzymes. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300701 |
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| Cardoso,Wilton Soares; Queiroz,Paula Viana; Tavares,Gabriella Peterlini; Santos,Fernando Almeida; Soares,Filippe Elias de Freitas; Kasuya,Maria Catarina Megumi; Queiroz,José Humberto de. |
| ABSTRACT The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Enzymes; Cellulases; Fungus; Hydrolysis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400879 |
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| Ribeiro,Cleberson; Cambraia,José; Peixoto,Paulo Henrique Pereira; Fonseca Júnior,Élcio Meira da. |
| The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H2O2 and O2•- contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H2O2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O2•- only in the roots of Fernandes cultivar. Increased lipid peroxidation was observed only in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aluminum; Enzymes; Reactive oxygen species; Oryza sativa; Oxidative stress. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200004 |
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| Shweta,Mishra; Agrawal,S.B.. |
| The impact of supplemental UV-B (sUV-B) radiation and heavy metals: Cd (68 µmol.kg-1 soil) and Ni (68 µmol.kg-1 soil) singly and in combination was studied on photosynthetic and non-photosynthetic pigments, metabolites, enzyme activities, biomass accumulation and the net primary productivity of spinach (Spinacia oleracea L. var. All Green), a common leafy vegetable plant. Both the stresses individually and in combination resulted in the reduction of photosynthetic pigments, ascorbic acid, and catalase activity, whereas a reverse trend was observed for anthocyanin, flavonoids and proline content, lipid peroxidation and peroxidase activity. The interactive effects of both the stresses were, however, less than additive. Combined treatment of sUV-B + Cd was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spinaicia oleracea; Biomass; Cadmium; Enzymes; Nickel; Pigments; Ultraviolet-B. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200007 |
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| Registros recuperados: 131 | |
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