|
|
|
| Registros recuperados: 227 | |
|
|
| Latchere, Oihana; Fievet, Julie; Lo, Cedrik; Schneider, Denis; Dieu, Stephanie; Cabral, Philippe; Belliard, Corinne; Ky, Chin-long; Gueguen, Yannick; Saulnier, Denis. |
| The present study investigated the effect of electrolysis on the biomineralization capacities of juveniles of the mollusk Pinctada margaritifera for the first time. Size-selected individuals from two groups, “Medium” and “Large”, from a multi-parental family produced in a hatchery system were subjected to electrolysis under a low voltage current over a nine-week experimental period. The growth of the juveniles was individually monitored and assessed weekly by wet weight and shell height measurements. At the end of the experiment, mantle tissue was sampled for biomineralization-related gene expression analysis. Electrolysis significantly increased pearl oyster growth in terms of shell height and wet weight for Large juveniles from the 5th and the 2nd week,... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Electrolysis; Biomineralization; Gene expression. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00343/45410/44924.pdf |
| |
|
|
| Tanguy, Arnaud; Boutet, Isabelle; Boudry, Pierre; Degremont, Lionel; Laroche, Jean; Moraga, Dario. |
| Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA... |
| Tipo: Text |
Palavras-chave: Gene expression; Xenobiotics; Mollusc; Phosphoglucomutase. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2220.pdf |
| |
|
| |
|
|
| Boulais, Myrina; Corporeau, Charlotte; Huvet, Arnaud; Bernard, Ismael; Quere, Claudie; Quillien, Virgile; Fabioux, Caroline; Suquet, Marc. |
| Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oocyte quality; Trochophore; Lipid; Gene expression; ATP. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00239/35010/33544.pdf |
| |
|
| |
|
|
| Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane. |
| To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to... |
| Tipo: Text |
Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf |
| |
|
|
| Gueguen, Yannick; Saulnier, Denis; Santini, Adrien; Magre, Kevin; Garen, Pierre; Bernagout, Solene; Nohl, Marine; Bouisset, Patrick; Helme, Herehia; Planes, Serge; Le Moullac, Gilles. |
| This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10 μg L− 1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM,... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; French Polynesia; Cadmium; Chromium; Gene expression; Metallothionein. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00376/48707/49146.pdf |
| |
|
| |
|
|
| Geffroy, Benjamin; Sadoul, Bastien; Bouchareb, Amine; Prigent, Sylvain; Bourdineaud, Jean-paul; Gonzalez-rey, Maria; Morais, Rosana N.; Mela, Maritana; Carvalho, Lucelia Nobre; Bessa, Eduardo. |
| Nature-based tourism is gaining extensive popularity, increasing the intensity and frequency of human-wildlife contacts. As a consequence, behavioral and physiological alterations were observed in most exposed animals. However, while the majority of these studies investigated the effects of punctual exposure to tourists, the consequences of constant exposition to humans in the wild remains overlooked. This is an important gap considering the exponential interest for recreational outdoor activities. To infer long-term effects of intensive tourism, we capitalized on Odontostilbe pequira, a short-lived sedentary Tetra fish who spends its life close to humans, on which it feeds on dead skin. Hence, those fish are constantly exposed to tourists throughout their... |
| Tipo: Text |
Palavras-chave: Coping style; Ecotourism; Conservation; Behavior; Gene expression; Cortisol; Neurogenesis; Fish. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53470/54366.pdf |
| |
|
| |
|
|
| Huvet, Arnaud; Daniel, Jean-yves; Quere, Claudie; Dubois, Solene; Prudence, Marie; Van Wormhoudt, A; Sellos, D; Samain, Jean-francois; Moal, Jeanne. |
| A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to estimate tissue-specific expression of two amylase genes in five different tissues of the Pacific oyster Crassostrea gigas and the experimental effects of food level on the cellular, enzymatic and gene expression changes of the digestive gland. Results indicated that both genes were transcribed at different levels depending on tissue type. A strong preferential expression of both amylase genes was observed in the digestive gland leading to high amylase activities, in accordance with the digestive function of this organ. Traces of gene expression and activity were observed in other analysed tissues. These appeared higher in labial palps than in other... |
| Tipo: Text |
Palavras-chave: Oyster; Gene expression; Food regulation; Digestive enzyme activity; Alpha amylase. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-394.pdf |
| |
|
| |
|
|
| Ovando, Fernanda; Gimpel, Carla; Cardenas, Constanza; Machado Cunha Da Silva, Jose Roberto; De Lorgeril, Julien; Gonzalez, Marcelo. |
| We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was... |
| Tipo: Text |
Palavras-chave: Antarctica; Sea urchin; Sterechinus neumayeri; Coelomocytes; Gene expression; AIF-1. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00098/20955/18572.pdf |
| |
|
|
| Sussarellu, Rossana; Lebreton, Morgane; Rouxel, Julien; Akcha, Farida; Riviere, Guillaume. |
| Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development. For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval... |
| Tipo: Text |
Palavras-chave: Oyster; Embryotoxicity; Copper; Gene expression; DNA methylation. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00417/52856/53756.pdf |
| |
|
|
| Fernandez, Ignacio; Lopez-joven, Maria-carmen; Andree, Karl B.; Roque, Ana; Gisbert, Enric. |
| Senegalese sole (Solea senegalensis) has been considered since the 1990´s to be a promising flatfish species for diversifying European marine aquaculture. However, pathogen outbreaks leading to high mortality rates can impair Senegalese sole commercial production at the weaning phase. Different approaches have been shown to improve fish immunocompetence; with this in mind the objective of the work described herein was to determine whether increased levels of dietary vitamin A (VA) improve the immune response in early juveniles of Senegalese sole. For this purpose, Senegalese sole were reared and fed with Artemia metanauplii containing increased levels of VA (37,000; 44,666; 82,666 and 203,000 total VA IU Kg-1) from 6 to 60 days post-hatch (early juvenile... |
| Tipo: Text |
Palavras-chave: Vitamin; Retinoic acid; Immune system; Gene expression; Senegalese sole Solea senegalensis; Photobacterium damselae subp. damselae. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38617/37147.pdf |
| |
|
|
| Avarre, Jean-christophe; Dugue, R.; Alonso, Pascal; Diombokho, A.; Joffrois, C.; Faivre, N.; Cochet, C.; Durand, Jean Dominique. |
| The black-chinned tilapia Sarotherodon melanotheron heudelotii is an ecologically appealing model as it shows exceptional adaptive capacities, especially with regard to salinity. In spite of this, this species is devoid of genomic resources, which impedes the understanding of such remarkable features. De novo assembly of transcript sequences produced by next-generation sequencing technologies offers a rapid approach to obtain expressed gene sequences for non-model organisms. It also facilitates the development of quantitative real-time PCR (qPCR) assays for analysing gene expression under different environmental conditions. Nevertheless, obtaining accurate and reliable qPCR results from such data requires a number of validations prior to interpretation.... |
| Tipo: Text |
Palavras-chave: Gene expression; Non-model organism; Reference gene; Salinity; Spermatogenesis; Tilapia. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00171/28255/26625.pdf |
| |
|
|
| Le Pabic, Lore; Parrad, Sophie; Sham Koua, Manaarii; Nakasai, Seiji; Saulnier, Denis; Devaux, Dominique; Ky, Chin-long. |
| Size is the most important and valuable quality of the cultured black-lip pearl, Pinctada margaritifera. As this pearl aquaculture is carried out at numerous grow-out sites, this study analyzes the environmental influence on pearl size parameters (nacre weight and thickness) in relation to the recipient oyster biometric parameters (shell thickness, height, width, and oyster weight) at harvest time. Toward this end, an experimental graft was designed by using a homogeneous donor oyster phenotype. The recipient oysters were randomly and equally transferred and reared in five commercial and contrasting grow-out locations. Overall inter-site comparisons revealed that the cultured pearl size (N=2168) and the biometric parameters of the recipient oysters were... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl size; Donor oysters; Recipient oysters; Gene expression; Environmental influences. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00321/43191/42738.pdf |
| |
|
| |
|
|
| MIURA, Ryotaro; HANEDA, Shingo; MATSUI, Motozumi; 羽田, 真悟; 松井, 基純. |
| The objective of our study was to compare the characteristics of the corpus luteum (CL) formed after ovulation of the dominant follicle (DF) of the first follicular wave (W1) and those of the CL formed after ovulation of the DF of the second (induced) follicular wave (W2). Non-lactating Holstein cows were used for this study. In Experiment 1, cows were treated with PGF2α and GnRH on days 6 and 8 (day 0 = day of follicular wave emergence) for W1 (n = 6) and W2 (n = 6), respectively. Dominant follicles were aspirated on day 9 to quantify the amounts of mRNA (VEGF120, VEGF164, FGF-2, StAR, P450-scc and 3β-HSD) in granulosa cells (GC). In Experiment 2, the size and blood flow area of the CL formed after ovulation of the DF in W1 (W1CL; n = 6) and W2 (W2CL; n =... |
|
Palavras-chave: Blood flow; Corpus luteum; Cow; Follicular wave; Gene expression. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4147 |
| |
|
|
| TAWFEEQ, Mohammad Monir; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽. |
| Expression of six selective genes in peripheral blood cells was evaluated as diagnostic biomarkers for enzootic bovine leukosis (EBL) by using 10 EBL and 15 clinically healthy cattle. The clinically healthy cattle generally showed lower gene expression levels. Although wide variations of gene expression were found in some clinical cases of EBL, 4 and 5 among 10 EBL cattle showed higher expression of interleukin 2 receptor gene (IL2R) and Wilms’ tumor gene (WT1), respectively. Expression of IL2R in peripheral blood cells in EBL cattle was statistically increased; however, the lower sensitivity and higher variation in the gene expressions among clinical cases of EBL would be problems as diagnostic biomarkers. |
|
Palavras-chave: Biomarkers; Enzootic bovine leukosis; Gene expression; QRT-PCR. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3868 |
| |
| Registros recuperados: 227 | |
|
|
|
