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Registros recuperados: 293 | |
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Taris, Nicolas; Sauvage, Christopher; Batista, Frederico; Baron, Sophie; Ernande, Bruno; Haffray, Pierrick; Boudry, Pierre. |
Previous studies have shown heritable variation in larval developmental traits in the Pacific oyster Crassostrea gigas. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors, specific to hatcheries, were examined: the effect of discarding the smallest larvae (i.e. culling) and the effect of temperature (20°C versus 26°C). A mixed-family approach was used in order to infer the genetic composition of larval populations and family assignment, limiting possible environmental bias and allowing the study of a relatively large number of families using a limited number of larval tanks. Our results show that three multiplexed highly polymorphic microsatellite markers are a powerful tool for family assignment and,... |
Tipo: Text |
Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-1505.pdf |
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Nezan, Elisabeth; Siano, Raffaele; Boulben, Sylviane; Six, Christophe; Bilien, Gwenael; Cheze, Karine; Duval, Audrey; Le Panse, Sophie; Quere, Julien; Chomerat, Nicolas. |
The family Kareniaceae is mostly known in France for recurrent blooms of Karenia mikimotoi in the Atlantic, English Channel, and Mediterranean Sea and for the unusual green discoloration in the saltwater lagoon of Diana (Corsica) caused by Karlodinium corsicum in April 1994. In terms of diversity, this taxonomic group was long overlooked owing to the difficult identification of these small unarmored dinoflagellates. In this study, thanks to the molecular characterization performed on single cells from field samples and cultures, twelve taxonomic units were assigned to the known genera Karenia, Karlodinium and Takayama, whereas one could not be affiliated to any described genus. The molecular phylogeny inferred from the D1–D2 region of the LSU rDNA showed... |
Tipo: Text |
Palavras-chave: Genetic diversity; Haemolytic; Kareniaceae; Pigments; Single cell; Taxonomy. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00225/33611/32310.pdf |
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Hillen, J. E. J.; Coscia, I.; Vandeputte, Marc; Herten, K.; Hellemans, B.; Maroso, F.; Vergnet, Alain; Allal, Francois; Maes, G. E.; Volckaert, F. A. M.. |
The aquaculture industry has increasingly aimed at improving economically important traits like growth, feed efficiency and resistance to infections. Artificial selection represents an important window of opportunity to significantly improve production. However, the pitfall is that selection will reduce genetic diversity and increase inbreeding in the farmed stocks. Genetic tools are very useful in this context as they provide accurate measures of genetic diversity together with many additional insights in the stock status and the selection process. In this study we assessed the level of genetic variability and relatedness over several generations of two lines of experimentally selected European sea bass (Dicentrarchus labrax L.). The first line was... |
Tipo: Text |
Palavras-chave: Artificial selection; DdRAD; Fish; Genetic diversity; Genomics; Inbreeding. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00392/50314/50993.pdf |
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Taris, Nicolas; Ernande, Bruno; Mccombie, Helen; Boudry, Pierre. |
The life histories of oysters in the genus Crassostrea, like those of most marine bivalves, are typified by high fecundity and low survival in nature. Rearing conditions in hatcheries however ensure optimized density, diet, and temperature. Hatcheries are becoming increasingly important for the production of juveniles in aquaculture, and their culture practices often include culling of slow growing larvae to reduce and synchronize the time taken to reach settlement. Because previous studies have found substantial genetic variation for early life developmental traits in Crassostrea gigas, these culling practices are likely to cause highly different selective pressures in hatcheries from those in the natural environment. We studied the phenotypic and genetic... |
Tipo: Text |
Palavras-chave: Oysters; Larvae; Hatchery; Genetic diversity; Culling; Crassostrea gigas. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1677.pdf |
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Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre. |
DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf |
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Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,... |
Tipo: Text |
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf |
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Teixeira, Sara; Cambon-bonavita, Marie-anne; Serrao, Ester A.; Desbruyeres, Daniel; Arnaud-haond, Sophie. |
Aim Deep-sea hydrothermal vents are unstable habitats that are both spatially and temporally fragmented. In vent species, a 'short-term insurance' hypothesis would lead us to expect mostly self-recruitment, limiting the loss of larvae in the deep ocean or water column and increasing genetic differentiation over the time elapsed since colonization. Alternatively, a 'long-term insurance' hypothesis would support the prediction of selection for large-scale dispersal, to ensure long-term persistence in these ephemeral habitats. The main goal of this study was to infer the spatial and temporal distribution of genetic diversity of the shrimp Rimicaris exoculata, which forms high-density local populations on hydrothermal vents along the Mid-Atlantic ridge.... |
Tipo: Text |
Palavras-chave: Bottleneck; Deep-sea biogeography; Dispersal; Genetic diversity; Hydrothermal vents; Marine biogeography; Mid-Atlantic Ridge; Rimicaris exoculata; Spatio-temporal distribution. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14394/12350.pdf |
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Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre. |
Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different... |
Tipo: Text |
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF |
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Moal, Jeanne; Bedier, Edouard; Fleury, Pierre-gildas; Langlade, Aime; Le Coguic, Yvette; Degremont, Lionel; Boudry, Pierre; Le Coz, Jean-rene; Pouvreau, Stephane; Enriquez-diaz, M.; Lambert, Christophe; Quere, Christele; Soudant, Philippe; Samain, Jean-francois. |
Bi-parental families were produced in hatchery and tested in the field in 2001. Two sets of 5 families were constituted, selected on their high (R) and low (S) survival. These two sets were reared in Brittany from March to November 2002. Samplings were performed twice a month to obtain data on biometry, survival, reproductive cycle, biochemical composition, adenylate energy charge, hemolymph parameters (ions and defense system) and muscle strength. "R" and "S" oysters exhibited different reproductive effort and spawning strategy. "R" oysters allocated less energy in gonad than "S" ones and presented a complete spawning at the end of August contrary to the "S" which spawns partially. Mortality started in July when the seawater temperature reached 19 degree... |
Tipo: Text |
Palavras-chave: Summer; Reproduction; Oyster culture; Mortality; Genetic diversity; Commercial species; Breeding. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3345.pdf |
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Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel. |
The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder... |
Tipo: Text |
Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf |
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Fromentin, Jean-marc. |
A recent body of literature has demonstrated that older females produce larvae and offsprings that have much higher survival and growth than do younger adults and that they furthermore play a key role in the adaptability, persistence and productivity of the exploited fish populations. These findings, that have been shown for several pelagic, groundfish and rockfish species, are, thus, worth to consider for tuna and tuna-like species. The aim of this modest and brief paper is solely to draw the attention from the SCRS to this issue which may have considerable impacts on management regulations. |
Tipo: Text |
Palavras-chave: Age structure; Genetic diversity; Recruitment success; Sustainability; Management. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00192/30333/28808.pdf |
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Soulsbury, Carl D.; Lebigre, Christophe. |
There is widespread interest in the relationship between individual genetic diversity and fitness-related traits (heterozygosity-fitness correlations; HFCs). Most studies have found weak continuous increases of fitness with increasing heterozygosity, while negative HFCs have rarely been reported. Negative HFCs are expected in cases of outbreeding depression, but outbreeding is rare in natural populations. Negative HFCs may also arise through viability selection acting on low heterozygosity individuals at an early stage producing a skew in the heterozygosity distribution. We tested this idea using survival and clutch parameters (egg mass, egg volume, chick mass, clutch size) in female Black Grouse Lyrurus tetrix and carried out simulations to determine how... |
Tipo: Text |
Palavras-chave: Inbreeding; Outbreeding; Neutral loci; Selective mortality; Genetic diversity. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53448/54340.pdf |
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Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in... |
Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf |
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Registros recuperados: 293 | |
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