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Relation between chemical composition of Grateloupia doryphora (Montagne) Howe, Gymnogongrus griffithsiae (Turner) Martius, and abiotic parameters Acta Botanica
Perfeto,Paulo Nelo Medeiros.
In Grateloupia doryphora and Gymnogongrus griffithsiae the seasonal variation of their chemical compounds was studied, establishing a relation with the physical and chemical properties of seawater. High values of proteins in the studied species were detected during the winter, 28.88% in G. doryphora and 26.68% in G. griffithsiae, corresponding to the maximum period of ammonium concentration in the marine environment. The variation in carbohydrates content showed an inverse relation with the proteins, with a maximum of 54.72% in G. doryphora and of 55.36% in G. griffithsiae, both in summer, positively correlated with salinity and temperature of sea water. Lipids content was low in both species. In G. doryphora the values ranged between 0.81 and 1.30% and,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algae; Proteins; Carbohidrates; Lipids; Ashes; Nitrogen; Phosphorus; Potassium.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33061998000100006
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Physiological and biochemical changes during desiccation tolerance loss in millet (Pennisetum glaucum L.) seeds Agronomy
Fernandes, Joana Souza; Guimarães, Renato Mendes; Faria, Jose Marcio Rocha; Pereira, Diego de Sousa; Coelho, Stefania Vilas Boas; Oliveira, Ariadne Santos.
The aim of this study was to evaluate the physiological and biochemical changes related to desiccation tolerance loss in millet seeds. The studied points of the germination process were determined according to the seed imbibition curve of the millet hybrid ADRF6010: control (0h), 3h of imbibition, 1 and 3 mm radicle. The seeds were dried on silica gel for 72h at 20°C, followed by pre-humidification at 25°C for 24h. Seed physiological quality was evaluated by electrical conductivity and a germination test, and seed vigor was evaluated with a first germination count and a germination speed index. The experiment was performed in a completely randomized design, and means were compared by the Scott-Knott test at a 5% probability. The enzymatic systems of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation sensitivity; Germination; Proteins; Enzymes.; Agronomy; Dessecação de sementes; Fisiologia de Sementes; Tolerância à dessecação em sementes.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36522
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Physiological and biochemical changes during the loss of desiccation tolerance in millet (Pennisetum glaucum L.) seeds Agronomy
Fernandes, Joana Souza; Guimarães, Renato Mendes; Faria, Jose Marcio Rocha; Pereira, Diego de Sousa; Coelho, Stefania Vilas Boas; Oliveira, Ariadne Santos.
The aim of this study was to evaluate the physiological and biochemical changes related to the loss of desiccation tolerance in millet seeds. The points studied were determined according to the seed imbibition curve of the hybrid ADRF6010: control (0 h), 3 h, 1 and 3 mm of radicle. The seeds were dried on silica gel for 72 h at 20 °C, followed by pre-humidification at 25 °C for 24 h. The physiological quality was evaluated by the electrical conductivity and germination test, and the seed vigor through the first count and germination speed index. The experiment was performed in a completely randomized design and means were compared by the Scott-knott test at 5% probability. The enzymatic systems evaluated were: superoxide dismutase, catalase, peroxidase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed Physiology; Desiccation Tolerance desiccation sensitivity; Germination; Proteins; Enzymes. Agronomy Dessecação de sementes; Fisiologia de Sementes Tolerância à dessecação em sementes.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36522
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Nutritional reserves of Vochysiaceae seeds: chemical diversity and potential economic uses Anais da ABC (AABC)
Mayworm,Marco A. S; Buckeridge,Marcos S; Marquez,Ursula M. L; Salatino,Antonio.
Contents of proteins, carbohydrates and oil of seeds of 57 individuals of Vochysiaceae, involving one species of Callisthene, six of Qualea, one of Salvertia and eight of Vochysia were determined. The main nutritional reserves of Vochysiaceae seeds are proteins (20% in average) and oils (21. 6%). Mean of carbohydrate contents was 5. 8%. Callisthene showed the lowest protein content (16. 9%), while Q. cordata was the species with the highest content (30% in average). The contents of ethanol soluble carbohydrates were much higher than those of water soluble carbohydrates. Oil contents lay above 20% for most species (30. 4% in V. pygmaea and V. pyramidalis seeds). The predominant fatty acids are lauric (Q. grandiflora), oleic (Qualea and Salvertia) or acids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodiesel; Carbohydrates; Fatty acids; Proteins; Seed oils; Vochysiaceae.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200012
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Proteins and Peptides at the Interfaces of Nanostructures Anais da ABC (AABC)
BRITO,ADRIANNE M.M.; BELLETI,ELISANGELA; MENEZES,LUCIVALDO R.; LANFREDI,ALEXANDRE J.C.; NANTES-CARDOS,ISELI L..
Abstract: The present review focuses on the proteins and peptides at the interfaces of nanostructured metals and semiconductors as a result of their use in synthesis in-situ and functionalization of nanostructures. We start the review with an introduction about the peculiar properties of nanostructured materials and their applications. In the following, the chemical and structural properties of peptides and proteins that allow their use as reducing, stabilizing, and functionalization agents are discussed. Proteins and peptides have not only the chemical groups for the metal ion reducing but also provide templates for directing the crystalline growing of nanostructures to the desired shapes and sizes. Proteins and peptides are also used mainly for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Controlled synthesis; Noble-metals; Nanomaterials; Catalysis; Proteins; Peptides.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700402
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Seed reserve composition and mobilization during germination and early seedling establishment of Cereus jamacaru D.C. ssp. jamacaru (Cactaceae) Anais da ABC (AABC)
Alencar,Nara L.M.; Innecco,Renato; Gomes-Filho,Enéas; Gallão,Maria Izabel; Alvarez-Pizarro,Juan C.; Prisco,José T.; Oliveira,Alexandre B. De.
Cereus jamacaru, a Cactaceae found throughout northeast Brazil, is widely used as cattle food and as an ornamental and medicinal plant. However, there has been little information about the physiological and biochemical aspects involved in its germination. The aim of this study was to investigate its reserve mobilization during germination and early seedling growth. For this, C. jamacaru seeds were germinated in a growth chamber and collected at 0, 2, 4, 5, 6, 8 and 12 days after imbibition for morphological and biochemical analyses. Dry seeds had wrinkled seed coats and large, curved embryos. Lipids were the most abundant reserve, comprising approximately 55% and 65% of the dry mass for cotyledons and the hypocotylradicle axis, respectively. Soluble sugars...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cactaceae; Carbohydrates; Cytochemistry; Lipids; Morphology; Proteins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300024
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Evaluation of protein extraction methods for enhanced proteomic analysis of tomato leaves and roots Anais da ABC (AABC)
VILHENA,MILCA B.; FRANCO,MÔNICA R.; SCHMIDT,DAIANA; CARVALHO,GISELLE; AZEVEDO,RICARDO A..
Proteomics is an outstanding area in science whose increasing application has advanced to distinct purposes. A crucial aspect to achieve a good proteome resolution is the establishment of a methodology that results in the best quality and wide range representation of total proteins. Another important aspect is that in many studies, limited amounts of tissue and total protein in the tissue to be studied are found, making difficult the analysis. In order to test different parameters, combinations using minimum amount of tissue with 4 protocols for protein extraction from tomato (Solanum lycopersicum L.) leaves and roots were evaluated with special attention to their capacity for removing interferents and achieving suitable resolution in bidimensional gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bidimensional electrophoresis; Proteins; Protocol optimization; Tomato.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401853
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Ion-specific thermodynamical properties of aqueous proteins Anais da ABC (AABC)
Lima,Eduardo R.A.; Biscaia Jr.,Evaristo C.; Boström,Mathias; Tavares,Frederico W..
Ion-specific interactions between two colloidal particles are calculated using a modified Poisson-Boltzmann (PB)equationandMonteCarlo(MC)simulations. PBequationspresentgoodresultsofionicconcentration profiles around a macroion, especially for salt solutions containing monovalent ions. These equations include not only electrostatic interactions, but also dispersion potentials originated from polarizabilities of ions and proteins. This enables us to predict ion-specific properties of colloidal systems. We compared results obtained from the modified PB equation with those from MC simulations and integral equations. Phase diagrams and osmotic second virial coefficients are also presented for different salt solutions at different pH and ionic strengths, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colloid stability; Hofmeister series; Potential of mean force; Proteins.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000100010
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Growth and biochemical composition of Chlorella vulgaris in different growth media Anais da ABC (AABC)
CHIA,MATHIAS A.; LOMBARDI,ANA T.; MELãO,MARIA DA GRAÇA G..
The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrates; Lipid class; Microalgae; Proteins.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401427
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Growth performance, morphometric analysis of the intestinal mucosa and thyroid of broiler fed canola meal Arq. Bras. Med. Vet. Zootec.
Moraes,P.O.; Novelini,L.; Krabbe,E.L.; Kawski,V.L.; Nunes,J.K.; Corcini,C.D.; Varella,A.S.; Xavier,E.G..
ABSTRACT The aim of this study was to evaluate the effect of replacing soybean meal with canola meal in broiler diets on performance, liver histopathology, morphometry of the intestinal mucosa and thyroid. One-day-old Cobb chicks (n=300) were distributed in a completely randomized design, with increasing levels of replacement of soybean meal with canola meal (0, 25, 50, 75 and 100%) and 6 repetitions of 10 birds each. Weight gain decreased linearly (P< 0.05) with increasing levels of inclusion of canola meal, in all stages. Villus height in the duodenum and jejunum linearly decreased (P< 0.05). Follicle diameter and thyroid follicular epithelium height increased linearly with increasing levels of canola meal (P< 0.05). Similarly, there was an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nutrition; Poultry; Proteins; Thyroid gland.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100187
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Pentoses Used in Cultures of Synechococcus nidulans and Spirulina paracas: Evaluation of Effects in Growth and in Content of Proteins and Carbohydrates BABT
Freitas,Bárbara Catarina Bastos de; Cassuriaga,Ana Paula Aguiar; Morais,Michele Greque de; Atala,Daniel Ibraim Pires; Costa,Jorge Alberto Vieira.
Abstract The biological assimilation of the sugars present in lignocellulosic residues has gained prominence since these residues are the most abundant and economic residues in nature. Thus, the objective of this work was to determine whether the use of D-xylose and L-arabinose as sources of carbon in Synechococcus nidulans and Spirulina paracas cultures affects the growth and production of proteins and carbohydrates. Kinetic growth parameters, pentose consumption, protein content and carbohydrates were evaluated. Synechococcus nidulans and Spirulina paracas consumed all concentrations of pentose used. The highest cellular concentration (1.37 g.L-1) and the highest protein productivity (54 mg.L-1.d-1) were obtained for Spirulina paracas, which was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arabinose; Proteins; Spirulina; Synechococcus; Xylose.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100513
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Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes BABT
Alves,Andrea Catão; Gontijo,Cristiano Machado; Oliveira,Mônica Cristina; Diniz,Simone Odília Fernandes; Oliveira,Flávia Márcia; Cardoso,Valbert Nascimento; Ramaldes,Gilson Andrade.
The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Liposomes; Oral absorption; Mucosal delivery; Proteins; Nanotechnology.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700035
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Temperature-dependent alterations in metabolic enzymes and proteins of three ecophysiologically different species of earthworms BABT
Tripathi,G; Kachhwaha,N.; Dabi,I.; Bandooni,N..
The effects of varying temperatures (12 - 44° C) on the specific activity of cytoplasmic malate dehydrogenase ((cMDH), mitochondrial malate dehydrogenase (mMDH) and lactate dehydrogenase (LDH) of some earthworms (Metaphire posthuma, Perionyx sansibaricus and Lampito mauritii) were studied. The effects of different temperatures on supernatant and mitochondrial protein contents were also investigated. The specific activities of cMDH, mMDH and LDH of the earthworms decreased gradually as a function of increasing temperature from 12 to 44°C. Higher metabolic energy was needed to maintain the activity at low temperatures. Hence, the earthworms showed increased enzyme specific activity at low temperatures. However, the protein content increased upto 28°C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymes; Proteins; Earthworms; Temperatures.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400017
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A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats BJMBR
Fernandes,A.A.; Faria,T. de O.; Ribeiro Júnior,R.F.; Costa,G.P.; Marchezini,B.; Silveira,E.A.; Angeli,J.K.; Stefanon,I.; Vassallo,D.V.; Lizardo,J.H..
Resistance training evokes myocardial adaptation; however, the effects of a single resistance exercise session on cardiac performance are poorly understood or investigated. This study aimed to investigate the effects of a single resistance exercise session on the myocardial contractility of spontaneously hypertensive rats (SHRs). Male 3-month-old SHRs were divided into two groups: control (Ct) and exercise (Ex). Control animals were submitted to sham exercise. Blood pressure was measured in conscious rats before the exercise session to confirm the presence of arterial hypertension. Ten minutes after the exercise session, the animals were anesthetized and killed, and the hearts were removed. Cardiac contractility was evaluated in the whole heart by the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cardiac; Contractility; Myocardium; Proteins; Resistance exercise.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900813
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Differences in pressure and temperature transitions of proteins and polymer gels BJMBR
Kunugi,S.; Kameyama,K.; Tada,T.; Tanaka,N.; Shibayama,M.; Akashi,M..
Pressure-driven and temperature-driven transitions of two thermoresponsive polymers, poly(N-isopropylacrylamide) (pNIPAM) and poly(N-vinylisobutyramide) (pNVIBA)), in both a soluble linear polymer form and a cross-linked hydro-gel form, were examined by a dynamic light-scattering method and direct microscopic observation, respectively. Their behavior was compared with that of protein systems. Changes in some characteristic parameters in the time-intensity correlation functions of dynamic light-scattering measurement of aqueous solutions of pNIPAM at various pressures and temperatures showed no essential differences during temperature and pressure scanning and, as a whole, the motions of polymers in aqueous solutions were similar in two types of transitions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pressure; Temperature; Denaturation; Transition; Proteins; Thermoresponsive polymers; Polymer gel.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800011
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Latex fluids are endowed with insect repellent activity not specifically related to their proteins or volatile substances Braz. J. Plant Physiol.
Ramos,Márcio V.; Araújo,Eliane S.; Oliveira,Raquel S. B.; Teixeira,Fabiano M.; Pereira,Danielle A.; Cavalheiro,Mariana G.; Souza,Diego P.; Oliveira,Jefferson S.; Freitas,Cleverson D. T. de.
Latex is an endogenous milky fluid synthesized and accumulated under pressure in a network of laticifer cells. In this study, latices from five plant species were examined for deterrent effect on oviposition of two Coleoptera (Bruchidae) pests. The latex from Euphorbia tirucalli, Calotropis procera and Plumeria rubrae exhibited deterrent activity on oviposition of both Callosobruchus maculatusand Zabrotis subfasciatus beetles. The latex from Cryptostegia grandiflora and Himathantus drasticus were less effective to C. maculatus and Z. subfasciatus oviposition, respectively. Eggs laid on latex-treated seeds were not affected.The emergence and the mean time of development and weight of larvae grown in treated seeds were similar to the controls. The deterrent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calotropis procera; Deterrent activity; Insect; Laticifers; Proteins; Plumeria rubra.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000100008
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Photo-oxidative stress in coconut seedlings: early events to leaf scorching and seedling death Braz. J. Plant Physiol.
Naresh,Kumar S.; Kasturi Bai,K.V..
Experiments were conducted on coconut seedlings to delineate events of photo-oxidative stress damage. Studies on chlorophyll fluorescence indicated a clear case of excess light energy under high light conditions causing stress to coconut seedlings raised under coconut palms. Quantum yield of photo-chemistry of leaflets exposed to high light was significantly less than those under shade. Seedlings exposed to high light and then shifted to shade have shown significant improvement in quantum yield. Excess light energy harvested by chlorophyll antenna caused high non-photochemical quenching resulting in production of biologically toxic super oxide, hydrogen peroxide and hydroxyl radicals. It is apparent that photoinhibition of photosynthesis takes place due to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucifera; Chlorophyll fluorescence; Oxidative stress; Photosynthesis; Lipid peroxidation; Gas exchange; Proteins.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300006
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Primary metabolism components of seeds from Brazilian Amazon tree species Braz. J. Plant Physiol.
Gonçalves,José Francisco de C.; Fernandes,Andreia V.; Oliveira,Antonio Fernando M.; Rodrigues,Lílian F.; Marenco,Ricardo A..
The contents of the main components of the primary metabolism (soluble sugars, starch, proteins, oils, fatty acids) and minerals (P, Ca, Mg, K, Fe, Zn, Mn, Cu) were characterized in seeds of five Brazilian Amazon tree species (Andira parviflora, Bertholletia excelsa, Helicostylis tomentosa, Hymenaea parviflora, and Parkia pendula). Soluble sugar contents were high in P. pendula seeds (14 %), whereas starch predominated in A. parviflora seeds (58.7 %). A. parviflora and H. parviflora seeds were rich in proteins (35.1 % and 32.4 %, respectively). The oil contents ranged from 1.4 % in A. parviflora to 70.7 % in B. excelsa. Only B. excelsa and P. pendula seeds may be considered oilseeds, with 70.7 % and 28.4 % oil, respectively. The fatty acid compositions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fatty acids; Minerals; Oils; Proteins; Soluble sugars; Starch.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200009
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Beverages formulated with whey protein and added lutein Ciência Rural
Rocha,Juliana de Cássia Gomes; Mendonça,Adriana Corrêa; Viana,Kéllen Wanessa Coutinho; Maia,Mariza de Paiva; Carvalho,Antônio Fernandes de; Minim,Valéria Paula Rodrigues; Stringheta,Paulo César.
ABSTRACT: This study aimed to develop and characterize beverages formulated with whey protein and added lutein. Beverages formulated with 0.5 (F1), 2.0 (F2), 4.0 (F3) and 6.0% w/v (F4) whey protein were physicochemically and microbiologically characterized, and sensory evaluated. The physicochemical analyses indicated that the protein content significantly changed (P<0.05) the acidity values, soluble solids and the colorimetric coordinates, making possible to adjust mathematical models for all these variables. Microbiological analyses showed no significant contamination (P<0.05) during processing that would compromise drinks quality of the drinks. Carotenoid content and the antioxidant activity did not change significantly (p>0.05) with increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant activity; Lutein; Proteins; Protein drink; Sensory acceptance; Whey.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300752
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Heparin-binding proteins of seminal plasma in Nellore bulls Ciência Rural
Fernandes,Carlos Eurico; Souza,Fabiana Ferreira de; Souza-Neto,Jayme Augusto; Ribola,Paulo Eduardo Martins.
The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Proteins; Seminal plasma; Bos taurus indicus.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048
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