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| Registros recuperados: 2.860 | |
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| Araújo,Karine Lourenzone; Rezende,Lucas Cunha Dias de; Souza,Letícia Soncini; Daltoé,Renata Dalmaschio; Madeira,Klesia Pirola; Paes,Marcela Ferreira; Herkenhoff,Fernando Luiz; Rangel,Letícia Batista Azevedo; Silva,Ian Victor. |
| The aim of this work was to study the estrogen receptor alpha (ERS1) PvuII and XbaI gene polymorphisms prevalence in randomly selected women population from Rio de Janeiro and Espírito Santo states in Brazil by polymerase chain reaction restriction fragment lengths polymorphism (PCR_RFLP) methodology. It was shown that Rio de Janeiro women exhibited a significantly different prevalence of XbaI polymorphism comparing to Espirito Santo women. Nonetheless, similar prevalence of PvuII polymorphism was found in both women´s populations. Moreover, a strong linkage disequilibrium was observed between these SNPs reinforcing the hypothesis of differential pattern of inheritance observed on such populations. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymorphism; Estrogen Receptor; Brazilian Women; Prevalence; Linkage disequilibrium. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600010 |
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| Dautania,Gajendra Kumar; Singh,Gajendra Pal. |
| The cyanobacterium Oscillatoria agardhii was isolated from the fresh water Mawatha lake, Jaipur and was grown in Zarrouk's medium at 25 ± 2°C, illuminated with white fluorescent light at the intensity of 2 500 lux with 12:12 h light and dark photoperiod. The effects of photoperiod and temperature on the growth and protein expression by Oscillatoria agardhii were studied under different controlled culture conditions (ALR, ALC, CLR, CLC, and NDL), measuring optical density, cell count and dry weight. Protein content was measured quantitatively by Bradford assay and qualitatively by SDS-PAGE. The densitometric analysis was also carried out for the measurement of the expression level of different proteins/peptides under different culture conditions. Maximum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bradford assay; Densitometer; Oscillatoria agardhii; Photoperiod; SDS-PAGE. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600933 |
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| Salmon,Denise Naomi; Piva,Luíza Cesca; Binati,Renato Leal; Vandenberghe,Luciana Porto de Souza; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Spier,Michele Rigon. |
| A new formulated product containing high yield of phytase from Schizophyllum sp., an important mushroom used for medicinal studies, was developed for application in feed industries and for future use in food processing. The enzyme presented a high activity yield 55.5 U/mL and 6240 U/gds in liquid and solid formulated product, respectively. It showed a good shelf-life in concentrated product, retaining 67.8% of its activity after 60 days of storage at room temperature and 90% of the activity was maintained in the liquid formulation after the same period. Powder bioformulated product maintained 77% of its activity after two months of storage, without the addition of chemical additives, which was named as a new bioformulated product containing high quantities... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phytase; Shizophyllum sp; Downstream steps; Stability; Formulation. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600001 |
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| Panarari-Antunes,Renata de Souza; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Galdino,Alexsandro Sobreira; Julio Junior,Horácio Ferreira; Prioli,Laudenir Maria. |
| This study aimed to evaluate the genetic variability of B. orbignyanus in cultivated and natural populations of the Upper Paraná River, using molecular RAPD markers and mtDNA control region. Specimens were collected in the Paraná River and in the Piracema fish farm in Maringá, State of Paraná, Brazil. RAPD primers produced 82 loci with consistent expression. The population from the Paraná River showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. Data revealed genetic differentiation between the two populations, although not very pronounced. These results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. The alignment of the D-loop sequences of B. orbignyanus indicated the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fish; Endangered; RAPD; MtDNA; Polymorphism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400025 |
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| Santos,Alexandre Martins Costa; Oliveira,Jamil Silvano de; Bittar,Eustáquio Resende; Silva,Anderson Lourenço da; Guia,Marcos Luiz dos Mares; Bemquerer,Marcelo Porto; Santoro,Marcelo Matos. |
| The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4ºC. The sample loading, salt concentration, flow rate and pH of mobile phase were varied to determine their effects on the resolution of the separation. The resolution was optimized mainly between β- and α-trypsin. Pure isoforms were obtained by chromatographying 100 mg of commercial trypsin during seven days, yielding 51 mg of high purity β-trypsin and 13 mg of α-trypsin partially pure, with small amounts of contaminating of ψ-trypsin.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypsin isoforms; Resolution; Ion-exchange chromatography; Purification; Specific activity; Mass spectrometry. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009 |
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| Das,Satya S.; Hall,Anne V.; Wareham,David W.; Britton,Keith E.. |
| Infection continues to be a major cause of morbidity and mortality worldwide. Nuclear medicine has an important role in aiding the diagnosis of particularly deep-seated infections such as abscesses, osteomyelitis, septic arthritis, endocarditis, and infections of prosthetic devices. Established techniques such as radiolabelled leucocytes are sensitive and specific for inflammation but do not distinguish between infective and non-infective inflammation. The challenge for Nuclear medicine in infection imaging in the 21st century is to build on the recent trend towards the development of more infection specific radiopharmaceuticals, such as radiolabelled anti-infectives (e.g. 99mTc- ciprofloxacin). In addition to aiding early diagnosis of infection, through... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Radiopharmaceuticals; Imaging; Infection; Inflammation; Radiolabelled anti-infectives; 99mTc- ciprofloxacin. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500005 |
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| Palestro,Christopher J.. |
| Nuclear Medicine plays an important role in the evaluation of patients suspected of harboring infection. Gallium imaging is especially useful for opportunistic infections and spinal osteomyelitis. In vitro labeled leukocyte imaging is the current radionuclide gold standard for imaging most infections, in immunocompetent patients, including cardiovascular, postoperative, and musculoskeletal infections (except spinal osteomyelitis). Several in-vivo leukocyte labeling methods have been investigated, but none are widely used. Results obtained with radiolabeled antibiotics have been disappointing. Data on FDG are still emerging, but this agent appears to be especially valuable in fever of unknown origin, spinal osteomyelitis, vasculitis and sarcoidosis. It is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infection; Labeled leukocytes; Gallium; FDG; PET. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700002 |
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| Berton Junior,João Francisco; Santos,Julio Cesar Pires; Coelho,Cileide Maria Medeiros; Klauberg Filho,Osmar. |
| The objective of this work was to evaluate the efficiency of nitrogen fixing inoculum associated with Co + Mo leaf spray on the common bean grain yield and grain nutrients, cv. FT Nobre. Three dosages of the inoculant (0, 200 and 400 g/50 kg seeds), combined with four Co + Mo leaf spray levels (T0=0,0; T1=4.9,49; T2=7.3,73; and T3=9.7,97 g ha-1 of Co and Mo, respectively) were tested. The grain yield with the use of the inoculant (400 g / 5O kg seed-1) associated with the higher level of Co+Mo (T2 and T3) was very similar to the mineral nitrogen condition fertilizer recommended for the bean (70 kg ha-1 of N). With the increased inoculant dosage, an increase of the protein content and of P and Mg in the grain was also observed. The results indicated that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seed quality; Phosphorus; Magnesium; Nitrogen. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600002 |
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| Zanuncio,José Cola; Beserra,Eduardo Barbosa; Molina-Rugama,Adrián José; Zanuncio,Teresinha Vinha; Pinon,Tobias Baruc Moreira; Maffia,Vanessa Pataro. |
| Reproduction and longevity of Supputius cincticeps (Stål) (Heteroptera: Pentatomidae) fed on Zophobas confusa Gebien, Tenebrio molitor L. (Coleoptera: Tenebrionidae) or Musca domestica (L.) (Diptera: Muscidae) larvae were studied during two generations at 24.7 ± 1.1ºC, 70 ± 10% R.H. and 12 h of photophase. Body weight of newly-emerged adults, oviposition period, number of egg masses, total number of eggs and longevity of S. cincticeps were higher when fed on Z. confusa or T. molitor larvae than on M. domestica larvae. Regardless of diet, S. cincticeps showed better reproduction and longevity in the second generation in laboratory conditions. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Asopinae; Predatory stinkbug; Fecundity; Alternative prey. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600013 |
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| Talbot,Jean-Nöel; Chevalme,Yanna-Marina. |
| This short review is dedicated to the current status of the assessment of a new PET radiopharmaceutical, fluoromethylcholine-(18F) or FCH, which is taken-up by prostate cancer tissue, in contrary to fluorodeoxyglucose-(18F) or FDG. It seems that FCH could become "the FDG of prostate cancer", with the same type of achievements (detection of distant metastases and of occult recurrences, restaging prior to invasive treatments), and the same drawbacks (false negative results in case of small lesions, in particular lymph nodes metastases, and false positive results in case of infection/inflammation, in particular prostatitis). Current evidence is summarised and discussed for each of the potential settings of FCH PET/CT imaging in prostate cancer. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fluoromethylcholine-(18F); PET/CT; Prostate cancer; Evaluation of radiopharmaceuticals. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700012 |
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| Registros recuperados: 2.860 | |
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