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Registros recuperados: 163
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Statistical optimisation of cell growth and carotenoid production by Rhodotorula mucilaginosa BJM
Maldonade,Iriani R.; Rodriguez-Amaya,Delia B.; Scamparini,Adilma R. P..
Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 2(5-1) experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2PO4. The nitrogen source (yeast extract) was the most important variable in enhancing carotenoid production; MgSO4.7H2O and KH2PO4 had a negative influence. The initial pH had no significant effect on carotenoid and cell productions. We further investigated the effects of glucose and yeast extract effects, using a second-order central composite design (CCD) to optimise carotenoid production, which was adequately approximated with a full quadratic equation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Torulene; Yeast; Response surface design; Rhodotorula mucilaginosa.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100012
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Ethanol production in Brazil: a bridge between science and industry BJM
Lopes,Mario Lucio; Paulillo,Silene Cristina de Lima; Godoy,Alexandre; Cherubin,Rudimar Antonio; Lorenzi,Marcel Salmeron; Giometti,Fernando Henrique Carvalho; Bernardino,Claudemir Domingos; Amorim Neto,Henrique Berbert de; Amorim,Henrique Vianna de.
ABSTRACT In the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in Brazil. These contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. Nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. Better control of fermentation conditions can reduce the stress conditions for yeast cells and contamination by bacteria and wild yeasts. There are great research opportunities in production processes of the first-generation ethanol regarding high-value added products, cost reduction and selection of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Yeast; Fermentation; Distillery.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500064
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Characteristics of Saccharomyces cerevisiae yeasts exhibiting rough colonies and pseudohyphal morphology with respect to alcoholic fermentation BJM
Reis,Vanda Renata; Bassi,Ana Paula Guarnieri; Silva,Jessica Carolina Gomes da; Ceccato-Antonini,Sandra Regina.
Among the native yeasts found in alcoholic fermentation, rough colonies associated with pseudohyphal morphology belonging to the species Saccharomyces cerevisiae are very common and undesirable during the process. The aim of this work was to perform morphological and physiological characterisations of S. cerevisiae strains that exhibited rough and smooth colonies in an attempt to identify alternatives that could contribute to the management of rough colony yeasts in alcoholic fermentation. Characterisation tests for invasiveness in Agar medium, killer activity, flocculation and fermentative capacity were performed on 22 strains (11 rough and 11 smooth colonies). The effects of acid treatment at different pH values on the growth of two strains ("52" -rough...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S. cerevisiae; Ethanol; Fermentation; Indigenous yeasts.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400014
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Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor BJM
Mukhtar,Hamid; Ikram-ul-Haq,.
We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0-8.0 and 8.0-10, respectively. Maximum production of proteases was observed at an incubation temperature of 37ºC while that of alpha amylase was observed at 40ºC. The optimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Simultaneous; Sporulation; Kinetics; Fermentation; Agitation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300033
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The use of Prevotella bryantii 3C5 for modulation of the ruminal environment in an ovine model BJM
Fraga,Martín; Fernández,Sofía; Perelmuter,Karen; Pomiés,Nicolle; Cajarville,Cecilia; Zunino,Pablo.
Abstract In the Southern Hemisphere, ruminants are mostly raised in grazing systems where animals consume forage and are supplemented with low amounts of concentrates. Concentrates are usually given separately and are rapidly ingested. This practice leads to changing rumen environment conditions during the day, may alter the rumen microbial metabolism and could affect host performance. The native ruminal Prevotella bryantii strain 3C5 was administered every 48 h to wethers under experimental conditions simulating Southern-Hemisphere feeding to evaluate its potential as a rumen fermentation modulator. The inoculum potential was assessed on day 17. The ammonia nitrogen (NH3-N), volatile fatty acids and ruminal pH were monitored on a 24-h basis 19 days after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotics; Rumen; Fermentation; Prevotella bryantii; Ovine model.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500101
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Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation BJM
Meneghin,Silvana Perissatto; Reis,Fabricia Cristina; Almeida,Paulo Garcia de; Ceccato-Antonini,Sandra Regina.
The ethanol production in Brazil is carried out by fed-batch or continuous process with cell recycle, in such way that bacterial contaminants are also recycled and may be troublesome due to the substrate competition. Addition of sulphuric acid when inoculum cells are washed can control the bacterial growth or alternatively biocides are used. This work aimed to verify the effect of chlorine dioxide, a well-known biocide for bacterial decontamination of water and equipments, against contaminant bacteria (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides) from alcoholic fermentation, through the method of minimum inhibitory concentration (MIC), as well as its effect on the industrial yeast inoculum. Lower MIC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorine dioxide; Bacteria; Yeast; Antibacterial agent; Alcohol; Fermentation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200026
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Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer BJM
Piccoli-Valle,Roberta Hilsdorf; Passos,Flávia Maria Lopes; Passos,Frederico José Vieira; Silva,Daison Olzany.
Penicillium griseoroseum was grown in bioreactors on mineral medium supplemented with yeast extract and sucrose. The influence of inoculum and carbon source concentrations, aeration and pH on pectin lyase (PL) production, as well as the capacity of P. griseoroseum to produce PL when grown on sugar cane syrup as carbon source were evaluated. Inoculum concentration did not influence PL production. Production was higher in non-aerated than in aerated medium. The best results were obtained using 60 mM sucrose at pH 6.3-7.2. Production using cane syrup 25% (v/v), without yeast extract supplement, was equal to that obtained under the conditions cited above.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Pectin lyase; Penicillium griseoroseum; Pectinases.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200013
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Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism BJM
Jiang,Yun-Gang; Wang,Chu-Yan; Jin,Chao; Jia,Jun-Qiang; Guo,Xijie; Zhang,Guo-Zheng; Gui,Zhong-Zheng.
DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mulberry; DNJ Production; Microorganisms; Fermentation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048
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Identification of lactic acid bacteria associated with traditional cachaça fermentations BJM
Gomes,Fatima C. O.; Silva,Carol L. C.; Vianna,Cristina R.; Lacerda,Inayara C. A.; Borelli,Beatriz M.; Nunes,Álvaro C.; Franco,Gloria R.; Mourão,Marina M.; Rosa,Carlos A..
During the production of traditional cachaça (alembic´s cachaça), contamination of the fermented must is one of the factors leading to economic losses in the beverage manufacturing industry. The diversity of bacterial populations and the role of these microorganisms during the cachaça production process are still poorly understood in Brazil. In our work, the fermentation process was followed in two distilleries located in the state of Minas Gerais. The objective of this work was to identify the populations of lactic acid bacteria present during cachaça fermentation using physiological and molecular methods. Lactic acid bacteria were isolated in high frequencies during all of the fermentative processes, and Lactobacillus plantarum and L. casei were the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Fermentation; Cachaça.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200031
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Effect of the presence of initial ethanol on ethanol production in sugar cane juice fermented by Zymomonas mobilis BJM
Tano,Marcia Sadae; Buzato,João Batista.
Ethanol production in sugar cane juice in high initial sugar concentration, fermented by Z. mobilis in the presence and absence of ethanol, was evaluated. Ethanol production was low in both media. The presence of initial ethanol in the sugar cane juice reduced ethanol production by 48.8%, biomass production by 25.0% and the total sugar consumption by 28.3%. The presence of initial ethanol in the medium did not affect significantly levan production and biomass yield coefficient (g biomass/g sugar consumed).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Sugar cane; Z. mobilis; Fermentation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300012
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Incidence and distribution of filamentous fungi during fermentation, drying and storage of coffee (Coffea arabica L.) beans BJM
Silva,Cristina Ferreira; Batista,Luis Roberto; Schwan,Rosane Freitas.
The objective of this work was to isolate and characterize filamentous fungi present in different stages of harvest, fermentation, drying and storage of coffee beans processed by natural method. The cherries were hand-picked and then placed on a cement drying platform where they remained until reached 11% of humidity. Microbial counts were found in all samples during fermentation and drying of the coffee beans. Counts of fungi in the coffee cherries collected from the tree (time 0) were around 1.5 x 10³ CFU/g. This number increased slowly during the fermentation and drying reaching values of 2 x 10(5) CFU/g within 22 days of processing. Two hundred and sixty three isolates of filamentous fungi were identified. The distribution of species during...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Filamentous fungi; Coffee; Toxigenic fungi.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300022
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Reducing lactate secretion by ldhA Deletion in L-glutamate- producing strain Corynebacterium glutamicum GDK-9 BJM
Zhang,Dalong; Guan,Dan; Liang,Jingbo; Guo,Chunqian; Xie,Xixian; Zhang,Chenglin; Xu,Qingyang; Chen,Ning.
L-lactate is one of main byproducts excreted in to the fermentation medium. To improve L-glutamate production and reduce L-lactate accumulation, L-lactate dehydrogenase-encoding gene ldhA was knocked out from L-glutamate producing strain Corynebacterium glutamicum GDK-9, designated GDK-9ΔldhA. GDK-9ΔldhA produced approximately 10.1% more L-glutamate than the GDK-9, and yielded lower levels of such by-products as α-ketoglutarate, L-lactate and L-alanine. Since dissolved oxygen (DO) is one of main factors affecting L-lactate formation during L-glutamate fermentation, we investigated the effect of ldhA deletion from GDK-9 under different DO conditions. Under both oxygen-deficient and high oxygen conditions, L-glutamate production by GDK-9ΔldhA was not higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corynebacterium glutamicum; L-glutamate; L-lactate dehydrogenase; Gene knockout; Fermentation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400044
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Alkali pretreated of wheat straw and its enzymatic hydrolysis BJM
Han,Lirong; Feng,Juntao; Zhang,Shuangxi; Ma,Zhiqing; Wang,Yonghong; Zhang,Xing.
The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Cellulose; Fermentation; Hydrolysis; Lignocellulosic; Pretreatment.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100006
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Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance BJM
Reis,Vanda Renata; Antonangelo,Ana Teresa Burlamaqui Faraco; Bassi,Ana Paula Guarnieri; Colombi,Débora; Ceccato-Antonini,Sandra Regina.
Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Stresses; Yeasts; Microsatellite; Contaminants.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268
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Screening of Metarhizium spp. strains for anticancer indolizidine alkaloid production and its rapid detection by MS analysis BJM
Justo,Oselys Rodriguez; Calheiros,Chrissana Santos; Perez,Victor Haber; Alegre,Ranulfo Monte.
Six fungi strains (M. anisopliae 3935, 4516, 4819, PL57, PL43 and M. flavoviride CG291) were studied regarding their ability to produce an anticancer indolizidine alkaloid. The culture process was carried out in Shaken flask at 26ºC and 200 rpm using three different culture medium containing oat meal extract supplemented with glucose and DL-lysine or Czapek culture medium. The mycelial extracts produced by Metarhizium spp. cultures were directly submitted to electrospray ionization mass spectrometry (ESI-MS) analysis and the highest alkaloid concentration (approximately, 6 mg.L-1) was reached when M. anisopliae 3935 was tested.
Tipo: Info:eu-repo/semantics/article Palavras-chave: M. anisopliae; M. flavoviride; Anticancer; Alkaloid; Fermentation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400035
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A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour BJM
Coelho,Sheila Lorena de Araújo; Magalhães,Valter Cruz; Marbach,Phellippe Arthur Santos; Cazetta,Marcia Luciana.
Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial enzyme; Fermentation; Agro-industrial substrates.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100120
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Molecular characterization of a Xylanase-producing fungus isolated from fouled soil BJM
Sakthiselvan,Punniavan; Naveena,Balakrishnan; Partha,Nagarajan.
Xylanase (EC 3. 2. 1. 8), hydrolyzes xylo-oligosaccharides into D-xylose and required for complete hydrolysis of native cellulose and biomass conversion. It has broad range of applications in the pulp and paper, pharmaceutical and Agri-food industries. Fifty fungal species were isolated from the fouled soil around an oil refinery and screened for the production of xylanase enzyme by enrichment culture techniques. The isolated fungal strain was identified as Hypocrea lixii SS1 based on the results of biochemical tests and 18s rRNA sequencing. The phylogenetic tree was constructed using the MEGA 5 software. Further, Hypocrea lixii SS1 was tested for the ability to utilize the sunflower oil sludge (waste from the oil industry) as the sole carbon source for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal isolate; Growth kinetics; Hypocrea lixii SS1; Fermentation; Xylanase; Purification.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400020
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Trehalose accumulation, invertase activity and physiological characteristics of yeasts isolated from 24 h fermentative cycles during the production of artisanal Brazilian cachaça BJM
Pataro,Carla; Guerra,Juliana B.; Gomes,Fátima C.O.; Neves,Maria J.; Pimentel,Patrícia F.; Rosa,Carlos A..
Trehalose accumulation, invertase activity and physiological characteristics of 86 yeast isolates from short fermentative cycles during the production of cachaça in three artisanal distilleries of the State of Minas Gerais were studied. Among these isolates, 70% were able to grow at temperatures between 40 and 42ºC. Only Saccharomyces cerevisiae isolates were able to grow over 40ºC. Lower temperatures (<40ºC) favoured the growth of yeasts such as Candida parapsilosis-like, C. maltosa-like, Kloeckera japonica, S. exiguus and C. bombicola-like. The isolates from all three distilleries were ethanol tolerant, produced invertase, and accumulate trehalose in the presence of glucose. The strains isolated from distillery A presented more resistance to ethanol...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Cachaça; Trehalose; Invertase; Fermentation.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300003
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Caffeine degradation by Rhizopus delemar in packed bed column bioreactor using coffee husk as substrate BJM
Tagliari,Cristiane Vanessa; Sanson,Raquel K.; Zanette,André; Franco,Telma Teixeira; Soccol,Carlos Ricardo.
Various microorganisms including bacteria, yeast and fungi can degrade caffeine. There are few publications about caffeine degradation pathway in filamentous fungi, mainly by solid-state fermentation (SSF). Studies were carried out on degradation of caffeine and their metabolites by filamentous fungi in SSF using coffee husk as substrate. The purpose of this work was to investigate the caffeine degradation pathway by Rhizopus delemar in packed bed column fermenter and to compare this degradation metabolism with glass flasks fermentation. The methylxanthines were quantified by HPLC analysis. The experiments were realized with the optimized conditions in previous experiments: pH 6.5, 28ºC, inoculation rate 10(6) spores/g substrate, aeration rate 60 mL/min...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decaffeination; Fermentation; Caffeine; Theophylline; Bioreactor; Filamentous fungi.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500035
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Biotechnological production of citric acid BJM
Max,Belén; Salgado,José Manuel; Rodríguez,Noelia; Cortés,Sandra; Converti,Attilio; Domínguez,José Manuel.
This work provides a review about the biotechnological production of citric acid starting from the physicochemical properties and industrial applications, mainly in the food and pharmaceutical sectors. Several factors affecting citric acid fermentation are discussed, including carbon source, nitrogen and phosphate limitations, pH of culture medium, aeration, trace elements and morphology of the fungus. Special attention is paid to the fundamentals of biochemistry and accumulation of citric acid. Technologies employed at industrial scale such as surface or submerged cultures, mainly employing Aspergillus niger, and processes carried out with Yarrowia lipolytica, as well as the technology for recovering the product are also described. Finally, this review...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citric acid; Fermentation; Review; Aspergillus niger.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400005
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