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| Registros recuperados: 227 | |
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| TAWFEEQ, Mohammad Monir; TAGAWA, Michihito; ITOH, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽. |
| A 49-month-old Holstein cow with anorexia, tachypnea, enlarged peripheral lymph nodes, and difficulty standing up was suspected of bovine leukosis. Hematological examination revealed lymphocytosis with the presence of neoplastic cells. Increased total lactate dehydrogenase (LDH) activity, isozymes of LDH-2 and LDH-3 activities and thymidine kinase activity were observed. Cytological findings of fine needle aspiration of subiliac lymph nodes indicated lymphosarcoma. Histopathology and antibody analysis confirmed the diagnosis of enzootic bovine leukosis, a B-cell bovine lymphoma caused by bovine leukemia virus. Gene expressions known as biomarkers of hematopoietic neoplasia in human were also examined in the present case. Increased messenger RNA expression... |
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Palavras-chave: Biomarker; Bovine leukosis; Gene expression. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4096 |
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| TAWFEEQ, Mohammad Monir; MIURA, Saori; NAKANISHI, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽. |
| A 12-month-old Holstein heifer with anorexia, lameness, and enlargement of peripheral lymph nodes was suspected of having bovine leukosis. Although lymphocytosis was not observed, cytology of fine needle aspirate from a superficial cervical node, and increased serum lactate dehydrogenase and thymidine kinase activities, strongly suggested lymphosarcoma. Increased numbers of mononuclear cells as well as mitotic cells were observed in synovial fluid collected from swollen joints. Pathological examination confirmed B-cell calf form bovine leukosis and joint swelling related to neoplastic cell infiltration. Both interleukin-2 receptor and thymidine kinase 1 genes were highly expressed in cells from superficial cervical lymph node aspirate. |
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Palavras-chave: Calf form bovine leukosis; Gene expression; Joint infiltration. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4097 |
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| NEPOMUCENO,ALEXANDRE LIMA; STEWART,JAMES MCD; OOSTERHUIS,DERRICK; TURLEY,RICKIE; NEUMAIER,MORMAN; FARIAS,JOSÉ RENATO BOUÇAS. |
| The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Messenger RNA; Gene expression; Genetic transformation; Water deficit. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700015 |
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| Castro,Maria Elita Batista de; Souza,Marlinda Lobo de; Sihler,William; Rodrigues,Júlio Carlyle Macedo; Ribeiro,Bergmann Morais. |
| Baculoviruses are insect viruses found mainly in Lepidoptera. The family Baculoviridae is taxonomically divided in two genera, Nucleopolyhedrovirus and Granulovirus, which differ by occlusion body morphology. NPVs (Nucleopolyhedroviruses) have polyhedrical inclusion bodies (PIBs) containing multiple viral particles, while GVs (Granuloviruses) appear to be generally single particles occluded in oval shaped occlusion bodies. During the life cycle, two different viral progenies are produced: BV (Budded Virus) and PDV (Polyhedra Derived Virus), which are essential for the infectious process and virus propagation in host cells. Baculoviruses are being used for pest control and they are especially safe due to their specificity and invertebrate-restricted host... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nucleopolyhedrovirus; AgNPV; Anticarsia gemmatalis; Baculovirus replication; Gene expression; Viral insecticide. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001000001 |
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| OLIVEIRA,C.; AGOSTINETTO,D.; LANGARO,A.C.; GARCIA,J.R.; LAMEGO,F.P.. |
| ABSTRACT: The global temperature to rise 0.3 to 4.8 oC to century. Supra-optimal temperatures can affect plants at different organizational levels, causing morphological, physiological, biochemical and gene expression alterations. Rice, weedy rice, and barnyardgrass may to response differently when subjected to supra-optimal temperatures. Thus, the aimed at determining the physiological response and expression of the genes APX2, HSP24.15 e HSP71.10 in rice, weedy rice, and barnyardgrass when in to supra-optimal temperatures. A greenhouse experiment was conducted in randomized complete desing with four repetitions, with a factorial combination of temperature x plantas, where: factor A consisted of two temperatures (25 oC and 40 oC); and factor B of three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; Echinochloa spp.; Photosynthesis; Oxidative stress; Gene expression. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100239 |
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| BENEMANN,D.P.; NOHATO,A.M.; VARGAS,L.; AVILA,L.A; AGOSTINETTO,D.. |
| ABSTRACT Real time reverse transcription polymerase chain reaction (RT-qPCR) is an important technique to analyze differences in gene expression due to its sensitivity, accuracy, and specificity. However, before analyzing the expression of the target gene, it is necessary to identify and evaluate the stability of candidate reference genes for the proper normalization. This study aimed at evaluating the stability of candidate reference genes in order to identify the most appropriate genes for the normalization of the transcription in rice and red rice in competition under different nitrogen levels, as well as to demonstrate the effectiveness of the reference gene selected for the expression of the cytosolic ascorbate peroxidase (OsAPX2). Eleven candidate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; Gene expression; Normalization. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100215 |
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| Wu,Y; Liang,H; Zhang,H; Pi,JS; Pan,AL; Shen,J; Pu,YJ; Du,JP. |
| ABSTRACT Ovoinhibitor (OIH) is the main proteinase inhibitor in the egg white. In the present study, real-time quantitative PCR and Western-Blot were used to analyze different expression pattern of OIH in ovaries as a candidate gene of reproductive traits in Jingjiang ducks (JJ ducks) and Shaoxing ducks (SX ducks) during three laying stages. To study the polymorphism of the OIH gene in those two duck populations, we designed five pairs of primers to detect SNPs of exon 3-5, 5-6, 14-16 and intron 7, 9 of the OIH gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA pool sequencing methods. The results showed that OIH expression increased during the laying stage in the ovaries of both duck breeds. The relative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laying ducks (Anas platyrhynchos); Laying period; Gene expression; OIH; Ovaries. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200281 |
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| Yaghobfar,A; Kalantar,M. |
| ABSTRACT The experiment was carried out in a completely randomized design (CRD) with 625 broiler chicks (Ross 308) for 5 repetitions (25 birds per each replicated) on the 5 treatments diet. Treatments included two different types of cereal grains (wheat, and barley) with or without an enzyme supplementation along with a corn-based diet as control group. The experimental diets were formulated to have similar contents of crude protein, metabolizable energy, total non-starch polysaccharides (NSP) and were fed in two periods of starter and grower. Experimental traits were consisted growth performance, ileal flora numeration, villus morphology in 3 parts of the intestine, digesta viscosity and pancreatic enzyme activity, and determining the gene expression... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broilers; Enzyme activity; Gene expression; Micro flora; NSP. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400629 |
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| Liu,Z; Xue,J; Luo,Y; Wang,Q; Zhong,H; Liang,M; Wang,C. |
| ABSTRACT The objective of the study was to investigate the mechanism by which dietary energy concentration regulates laying performance in geese. Eighty 558-day-old female Sichuan White geese were randomly allotted to two dietary treatments, each treatment was fed 1 of 2 experimental diets containing 10.00 (deficient) or 11.80MJ/kg metabolizable energy (sufficient) for 30 days. Laying performance, hormone concentration and gene expressions in hypothalamus-pituitary-gonadal axis were examined in geese. Birds fed the sufficient-energy diet had significantly higher average egg weight, daily laying rate, and lower feed to egg ratio than those fed the deficient-energy (p<0.05). The birds fed sufficient-energy diet had higher concentration of serum insulin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Energy concentration; Laying geese; Reproductive hormone; Gene expression. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300306 |
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| Ma,YF; Chen,L; He,J; Tian,Y; Xu,XQ; Du,X; Lu,LZ. |
| ABSTRACT Ten-week-old Langde geese in similar body weight were randomly selected, four for overfeeding and four for routinly feeding. The abundance of liver fatty acid-binding protein (L-FABP), thyroid hormone-responsive (THRSP or Spot 14), obese (OB), and apolipoprotein A1 (Apo A1) genes in goose were detected by quantitative RT-PCR. L-FABP was higher expressed in liver and intestine than other tissues, but no expression was detected in the pancreas or brain. The other three genes were widely expressed in different tissues, OB was higher expressed in pancreas and abdominal fat, Spot 14 and Apo A1 was higher expressed in sebum and abdominal fat. Spot 14 and Apo A1 genes were up-regulated in overfed goose livers compared with that in the control. Thus, Spot... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Langde goose; Lipids related genes; Gene expression; Fatty liver. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000200305 |
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| Nie,CX; Zhang,WJ; Ge,WX; Liu,YF; Wang,YQ; Liu,JC. |
| ABSTRACT Fermented cottonseed meal (FCSM) is widely used in poultry diets in China. This study was conducted to investigate the effect of FCSM on lipid-related gene expression in broilers. Initially, 180 broiler chickens (21-days-old, equal number of males and females) were randomly divided into three groups, with six pens per group and 10 birds per pen. The chickens in the control group were fed a diet containing unfermented cottonseed meal, and those in the treatment groups were fed with diets including either CSM fermented by Candida tropicalis (Ct group) or CSM fermented by Candida tropicalis plus Saccharomyces cerevisae (Ct-Sc group) until 64 days old. The results revealed that, compared with the control group (p<0.05), the mRNA expression of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fermented cottonseed meal; Fermented feed; Lipid metabolism; Gene expression; Broilers. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015001000057 |
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| Onbaşilar,EE; Erdoğan,M; Erol,S; Kahraman,M; Güngör,ÖF. |
| ABSTRACT Pure breeds are important due to the fact that they adapt well to poor husbandry conditions. However, gene expression of digestive enzymes and nutrient transporters of pure breeds and layer hybrids have not been studied yet. Therefore, this study was directed to detect the gene expression of digestive enzymes and nutrient transporters in the yolk sac membrane and small intestine of the embryos in layer hybrid and pure breeds. A total of 354 hatching eggs were collected from 29 wk old Denizli, Gerze and Lohman White (LW) breeder flocks. Twelve eggs from each genotype were selected at embryonic day 19 (E19) and day 21 (E21). APN, SI, PepT1, EAAT3, CAT1, B0AT, SGLT1 and GLUT5 mRNA expressions were evaluated in the yolk sac membrane (YSM) and small... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Gene expression; Layer hybrid; Pure breed. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300435 |
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| José,Anderson C.; Ligterink,Wilco; Davide,Antonio Claudio; Silva,Edvaldo A. Amaral da; Hilhorst,Henk W.M.. |
| Seeds of Magnolia ovata were dried to different water contents to assess the viability and transcript abundance of genes related to seed development, cell cycle, cytoskeleton and desiccation tolerance.The expression of development, cell cycle and cytoskeleton relative genes (ABI3, CDC2-like and ACT2) alone could not explain the germination behaviour of M. ovata seeds in relation to drying damage. Irrespective of their initial water content, the seeds performed in the same way during the initial period of germination and the deleterious effects of desiccation only occurred in later stages. Expression of PKABA1, sHSP17.5 and 2-Cys-PRX did not show a relationship with desiccation. However, the expression patterns of PKABA1 and sHSP17.5 suggested the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Desiccation tolerance; Drying; Gene expression; Magnolia ovata. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222009000100030 |
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| Zhou,Chuanpeng; Ge,Xianping; Liu,Bo; Xie,Jun; Chen,Ruli. |
| The effect of dietary carbohydrate (CHO) level on serum physiological response, hepatic antioxidant abilities and heat shock protein 70 (HSP70) expression of Wuchang bream (Megalobrama amblycephala) was studied. Two isonitrogenous (28.56% crude protein) and isolipidic (5.28% crude lipid) diets were formulated to contain 30% or 53% wheat starch. Diets were fed for 90 days to fish in triplicate tanks (28 fish per tank). At the end of feeding trial, significantly higher serum triglyceride level, insulin level, cortisol level, and malondialdehyde (MDA) content were observed in fish fed the 53% CHO diet, while significantly lower serum total protein content, alkaline phosphatase activity, superoxide dismutase activity and total antioxidative capacity were found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biochemical parameters; Gene expression; Herbivorous fish; Immunity; Starch. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001200627 |
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| Ding,Ke; Wang,Jianping; Liu,Ning; Zhang,Feike. |
| ABSTRACT This study aimed to investigate the effect of Artemisia apiacea Hance supplementation on growth performance, cecal opportunistic bacteria, and antimicrobial defense using 120 rabbits. There were four experimental diets containing a control and A. apiacea Hance added at doses of 25, 50, and 75 g kg−1 of feed. The trial lasted for 70 days. The results showed that diets supplemented with A. apiacea Hance improved feed intake, body weight gain, and feed efficiency. Linear and quadratic responses were found between feed intake and herbal meal doses. For cecal opportunistic pathogenic bacteria, compared with the control treatment, the herb decreased cecal C. perfringens, Gram-negative bacteria, and Salmonella spp. by 9.5 to 56.8%. Linear responses of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C. perfringens; Gene expression; Salmonella. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100533 |
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| Registros recuperados: 227 | |
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