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| Registros recuperados: 425 | |
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| Asssunção,I.P.; Listik,A.F.; Barros,M.C.S.; Amorin,E.P.R.; Silva,S.J.C.; O. Silva,Izael; Ramalho-Neto,C.E.; Lima,G.S.A.. |
| Os Begomovirus fazem parte de uma família numerosa de fitovírus denominada Geminiviridae. Eles infectam ampla gama de hospedeiras, incluindo muitas espécies cultivadas, como tomate (Lycopersicon esculentum), feijão (Phaseolus vulgaris), pimentão (Capsicum annuum), caupi (Vigna unguiculata), mandioca (Manihot esculenta) etc., além de plantas invasoras de várias espécies. Em alguns casos, plantas invasoras podem funcionar como reservatórios desses vírus para plantas cultivadas, mediante transmissão pelo inseto-vetor. No presente trabalho, plantas invasoras com sintomas de mosaico amarelo, deformação do limbo foliar e redução do crescimento foram avaliadas no tocante à presença de Begomovirus mediante a técnica de PCR, empregando-se oligonucleotídeos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Geminiviridae; Detecção; PCR; Hospedeiras daninhas. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582006000200005 |
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| SILVA,D.R.O.; AGOSTINETTO,D.; VARGAS,L.. |
| ABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in electrophoresis. Biotypes were identified as Conyza bonariensis and microcephala varieties. In molecular analysis, among 25 primers RAPD, eight were reproducible... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conyza bonariensis; Polymorphism; Glyphosate; PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433 |
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| Yaman,S; Sahan Yapicier,O. |
| ABSTRACT Gallibacterium anatis, a member of the Pasteurellaceae family, leads to decrease in egg-production, animal welfare and increase in mortality. This study aimed to diagnose G. Anatis, which caused economic losses in laying hens by using conventional and molecular techniques. In this study, G. anatis was examined from a total of 200 dead chicken tissues (heart, liver, lung, spleen and trachea) in laying hen farms that observed a decrease in egg production with respiratory system infection. Conventional methods based on colony morphology, sugar fermentation tests and hemolytic properties and molecular conformation using 16S rRNA-23S rRNA specific primers were performed to identify G. anatis. G. anatis was isolated in 20 (10%) of the examined samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallibacterium anatis; Isolation and identification; Layers; PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300307 |
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| Silva,EN. |
| The infectious bronchitis virus (IBV) was detected for the first time in Brazil by Hipólito in 1957 in chickens sold life in the municipal market of Belo Horizonte, MG, when commercial poultry production was just starting in that country. The Massachusetts (Mass) serotype was identified. However, the clinical disease was only observed in 1975, when poultry production was intensely growing. The extensive outbreak produced the classical condition in layers and breeders, affecting egg production and quality, whereas broilers presented respiratory and "nephritis-nephrosis" signs. The disease rapidly spread to all poultry-producing regions in the country, and in 1979, both the imports and the manufacturing of live vaccines against IB strains Mass, H120 and H52,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Genotyping; Infectious Bronchitis; PCR; Vaccination; Variants. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000300009 |
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| Aksoy,A; El Kahlout,KEM; Yardimci,H. |
| ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Disinfectants; PCR; Poultry; Resistance; Salmonella. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100301 |
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| Gomes,AM; Costa,LL; Vilela,DAR; Marques,MVR; Carvalhaes,AG; Marin,SY; Costa,MP; Horta,RS; Resende,JS; Martins,NRS. |
| Mycoplasma gallisepticum (Mg) infection of wild native Brazilian psittacines (Psittaciformes) which died of any cause during sorting, rehabilitation, or conservation, was investigated by PCR. Two previously described PCR methodologies using Mg specific primers were employed for the analyses of 140 swab samples (cloaca, trachea, or palatine cleft). Average positive Mg detection in cloacal swabs was 51.9%, with 80.0% (n=5) of Blue-and-yellow Macaws (Ara ararauna), 60.0% (n=3) Dusky Parrots (Pionus fuscus), 52.5% (n=59) Amazon Parrots (Amazona aestiva), 50.0% (n=2) Orange-winged Parrots (Amazona amazonica), 50.0% (n=2) Jandaya Parakeetsor Jandaya Conures (Aratinga jandaya), 0% (n=2) Golden Conures or Golden Parakeets (Guarouba guarouba), and 0% (n=2) Hyacinth... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazona aestiva; Amazona amazonica; Anodorhynchus hyacinthinus; Aratinga jandaya; Brazilian psittacine fauna; Guarouba guarouba; Mycoplasma gallisepticum; PCR; Pionus fuscus; Psittaciformes; Psittacidae. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200001 |
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| Li,X; Liu,L; Li,Q; Xu,G; Zheng,J. |
| ABSTRACT Salmonella enterica subspecies enterica serotype Enteritidis (SE) has caused foodborne infections over decades. It is transmitted mainly from contaminated eggs to humans. SE is commonly present in layer houses, and closely interacts with environmental factors. The objective of the present study was to develop a viable PCR method to identify SE in environmental samples collected in layer farms of different sizes, and to evaluate SE contamination status in four main egg-production provinces of northern China. After specificity retrieval using Primer-BLAST against the NCBI database, three SE specific oligonucleotide primers were selected as candidate primers. The primers targeting Prot6e gene were adopted and primers targeting Sdf I were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Environment; Layer farm; PCR; Salmonella Enteritidis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300377 |
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| Villarreal,LYB; Brandão,PEB; Chacón,JLV; Doretto Junior,L; Ito,N; Gama,NS; Ishizuka,MM; Luchese,A; Buchala,F; Astolfi-Ferreira,CS; Ferreira,AJP. |
| Avian Infectious Laryngotracheitis, caused by Infectious Laryngotracheitis Virus (ILTV), has been reported for decades in Brazilian laying and broiler flocks. More recently, outbreaks have occurred in São Paulo State. This study reports the application of PCR and DNA sequencing targeted to the p32 gene of ILTV using laying chicken samples from Bastos, São Paulo, Brazil. Three out of four field samples were positive by PCR. DNA sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of ILTV. The results definitely confirmed the presence of ILTV in the birds during the outbreak. Further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian infectious laryngotracheitis; Brazil; P32; PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400011 |
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| Fiorentin,L; Mores,MAZ; Trevisol,IM; Antunes,SC; Costa,JLA; Soncini,RA; Vieira,ND. |
| There is a need for a better understanding of the epidemiology of Mycoplasma synoviae (MS) infection in broiler breeders in Brazil. Many features of the infection remain unrecognizable, because there are no clinical signs of the disease. A detailed testing was performed at each 6 to 8 weeks in three MS-free flocks introduced in farms with endemic MS infection for a follow-up epidemiological study. Every flock was monitored by polymerase chain reaction (PCR), by serum plate agglutination (SPA) and hemagglutination inhibition (HI) for serology studies, and isolation of mycoplasmas from tracheal swabs. PCR was found to be the most sensitive test, detecting early MS infection. Serology was positive in less than 50% of the sera and MS was isolated only between... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler breeders; Mycoplasma synoviae; PCR; Serology; Survey; Transmission. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100005 |
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| Torre,D De la; Nuñez,LFN; Puga,B; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP. |
| ABSTRACT Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken parvovirus; Enteric diseases; Molecular diagnostic; PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400643 |
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| Vargas,Luciano Kayser; Lisboa,Bruno Brito; Scholles,Dércio; Silveira,José Ricardo Pfeifer; Jung,Gabriela Cardoso; Granada,Camille Eichelberger; Neves,Andrei Gibbon; Braga,Marcos Martins; Negreiros,Tatiana. |
| A acácia-negra é a terceira espécie florestal mais cultivada no Brasil. Além de sua importância econômica, é utilizada na recuperação de áreas degradadas, nas quais o solo geralmente apresenta pH baixo e altos teores de Al. O presente trabalho objetivou avaliar a diversidade genética de rizóbios naturais de solos do Rio Grande do Sul e selecionar isolados eficientes na fixação de N2 em condições de pH baixo. Um total de 50 isolados de Bradyrhizobium sp. foi obtido, os quais, juntamente com as estirpes recomendadas BR 3067 e BR 3068, foram caracterizados com o marcador BOX A 1-R. O padrão de bandas dos isolados foi utilizado na construção de um dendrograma, a partir do qual se calculou o índice de diversidade de Shannon. Dez isolados foram testados quanto à... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fixação biológica de nitrogênio; PCR; Rhizobium; Acacia mearnsii. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832007000400005 |
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| Silva,Sarah Cavalcanti da; Assunção,Iraildes Pereira; Carnaúba,Juliana Paiva; Lima,Joyce Silva; Amorim,Edna Peixoto da Rocha; Lima,Gaus de Andrade. |
| O maracujazeiro é uma cultura de grande importância econômica nos países tropicais, destacando-se o Brasil como o maior produtor. No presente trabalho, é relatada a infecção de plantas de maracujazeiro por um vírus do gênero Begomovirus, família Geminiviridae, no município de Anadia, Estado de Alagoas. As plantas infectadas apresentavam sintomas de mosaico amarelo, redução drástica de crescimento e da área foliar. DNA extraído de plantas sintomáticas foi empregado como molde em PCRs, contendo os pares de primers PAL1v1978 e par1C496 ou PBL1v2040 e PCRc1 que direcionam, respectivamente, a amplificação de regiões de aproximadamente 1,2 kb do DNA-A e 0,6 kb do DNA-B, do genoma dos begomovírus. Fragmentos de tamanho esperado foram amplificados a partir de... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Geminividae; Diagnose; PCR. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300037 |
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| Registros recuperados: 425 | |
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