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Registros recuperados: 8.210
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Predicting regulatory elements in repetitive sequences using transcription factor binding sites Electron. J. Biotechnol.
Horng,Jorng-Tzong; Cho,Wen-Fu.
Repeat sequences are the most abundant ones in the extragenic region of genomes. Biologists have already found a large number of regulatory elements in this region. These elements may profoundly impact the chromatin structure formation in nucleus and also contain important clues in genetic evolution and phylogenic study. This study attempts to mine rules on how combinations of individual binding sites are distributed repeat sequences. The association rules mined would facilitate efforts to identify gene classes regulated by similar mechanisms and accurately predict regulatory elements. Herein, the combinations of transcription factor binding sites in the repeat sequences are obtained and, then, data mining techniques are applied to mine the association...
Tipo: Journal article
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000300004
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Funding of human stem cell research by the United States Electron. J. Biotechnol.
Jones,Phillip B.C..
During the fall of 1998, three research groups announced that they had devised methods for obtaining human embryonic stem cells. Human stem cells are characterized by the ability to form many cell types, and the scientific community anticipates that the cells should be useful for the treatment of a wide variety of diseases. The dilemma is that human stem cell methodology can require, at least to some extent, research with human fetal tissue or research involving human embryos. Those opposed to federal funding of the former are concerned that the studies would support the "legitimacy" of abortion, while the latter is seen as prohibited under current law. The National Bioethics Advisory Committee has suggested that the federal government should fund both...
Tipo: Journal article
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000100003
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Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida Electron. J. Biotechnol.
Spollansky,Tatiana C.; Pitta-Alvarez,Sandra I.; Giulietti,Ana M..
Hairy root cultures of Brugmansia candida (Solanaceae), a South American plant which produces scopolamine and hyoscyamine, were exposed to different elicitors (jasmonic acid (JA) and aluminum chloride (AlCl3)) in order to increase their productivity and/or stimulate their liberation. Hairy roots of 19-day old cultures (exponential phase) were exposed to these elicitors for 24 and 48 hours. The effects on alkaloid accumulation and release into the medium were evaluated. JA was tested at 2.5 and 25 m g/ml. After 24 hours, JA promoted the release of hyoscyamine (~1200%) when the highest concentration was used. Therefore, the positive effects seen with JA could possibly be attributed in part to ethanol (EtOH), the solvent in which the acid was dissolved. At...
Tipo: Journal article
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000100006
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AÇÕES DE POLÍTICAS PÚBLICAS PARA O NOVO RURAL BRASILEIRO SOB O ENFOQUE NO DESENVOLVIMENTO LOCAL ExtensaoRural
Campanhola, Clayton; Silva, José Graziano da.
Disponível em anexo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Política pública desenvolvimento rural; Desenvolvimento local; Participação.
Ano: 2000 URL: http://periodicos.ufsm.br/extensaorural/article/view/6167
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ANÁLISE DA SUSTENTABILIDADE DE CONDOMÍNIOS RURAIS FORMADOS POR AGRICULTORES FAMILIARES ExtensaoRural
Olivo, Clair Jorge; Possamai, Osmar.
Disponível em anexo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroecologia; Sistemas de produção; Metodolodia de análise.
Ano: 2000 URL: http://periodicos.ufsm.br/extensaorural/article/view/6170
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A GESTÃO AGRÍCOLA E OS PLANTIOS FLORESTAIS COMO ALTERNATIVA DE RENDA DOS PRODUTORES RURAIS NO SUL DO BRASIL ExtensaoRural
Dossa, Derli; Rodigheri, Honorino Roque.
Disponível em anexo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Administração rural; Agrofloresta; Pequena produção.
Ano: 2000 URL: http://periodicos.ufsm.br/extensaorural/article/view/6168
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ANÁLISE DAS MARGENS DE COMERCIALIZAÇÃO DO SETOR ORIZÍCOLA GAÚCHO ExtensaoRural
Ereias, Ana Cristina Silveira; Waquil, Paulo Dabdab.
Disponível em anexo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arroz; Margem de comercialização e preço.
Ano: 2000 URL: http://periodicos.ufsm.br/extensaorural/article/view/6171
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O CADASTRO TÉCNICO MULTIFINALITÁRIO RURAL (CTMR): UMA FERRAMENTA PARA PROMOVER O DESENVOLVIMENTO LOCAL SUSTENTÁVEL ExtensaoRural
Loch, Carlos; Neumann, Pedro Selvino; Bernardy, Rógis Juarez; Dalotto, Roque A. Sanchéz; Zampier, Sérgio Luiz; Seiffert, Walter Quadros.
Disponível em anexo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desenvolvimento e planejamento rural; Sistemas de informação; Cadastro técnico multifinalitário; Gestão territorial; Santa Catarina.
Ano: 2000 URL: http://periodicos.ufsm.br/extensaorural/article/view/6169
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STUDIES ON THE ECOLOGY OF LARVAL MYCTOPHIDS (PISCES, MYCTOPHIDAE): A PROPOSAL FOR NEW RESEARCH LINES IN THE HUMBOLDT CURRENT Gayana
Rodríguez,Laura; Castro,Leonardo.
This is a review on some biological and ecological characteristics of the early life stages of myctophids that exemplify their adaptations to coastal upwelling zones and oceanic nearby areas. The approach has been, however, not to make an exhaustive list of all possible research subjects but, in a more pragmatic way, a) to identify relevant aspects that can be approached with currently available methods in the region and, b) through examples of studies published nationally and regionally, to stimulate future research in larval ecology in the Humboldt Current. Three major themes were assessed: taxonomy and systematics, population ecology and reproductive strategies and, ecophysiology of the early life stages. Among the former, we detected a lack of...
Tipo: Journal article Palavras-chave: Ichthyoplankton; Myctophids; Humboldt Current; Upwelling areas.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200011
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Cu, Pb AND Zn DISTRIBUTION IN NEARSHORE WATERS IN SAN JORGE BAY, NORTHERN CHILE Gayana
Salamanca,Marco A.; Camaño,Andrés; Jara,Bibiana; Rodríguez,Tatiana.
An extensive spatial survey metal content to study the spatial distribution of Cu, Pb and Zn concentration in nearshore waters of San Jorge Bay, along the coast of Antofagasta City, Chile was carried out located near the Atacama desert, which represent the largest populated urban settlement in northern Chile (~ 260,000 inhabitants). The coastal zone of this area is under an intensive use as discharge location of pollutants of different sources. Dissolved Cu varies between 0.97 to 1.65 mug l-1, in the southern part of the study area and in front of an industrial discharge, respectively. The dissolved Pb shows a narrow concentration range (0.019 to 0.029 mug l-1) and Zn content varies between 1.7 mug l-1 in the northern part of the study area to 2.0 mug l-1,...
Tipo: Journal article Palavras-chave: Trace metal distribution; Coastal waters; Desert areas.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200009
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QUANTITATIVE KARYOTYPE OF DIPLODON CHILENSIS (GRAY 1828) (BIVALVIA:HYRIIDAE) Gayana
Jara-Seguel,Pedro; Peredo,Santiago; Palma-Rojas,Claudio; Parada,Esperanza; Lara,Gladys.
The karyotype of a freshwater population of Diplodon chilensis located in La Poza area of the Villarrica Lake (39º18'S; 72º05'W), Southern Chile, was studied. The chromosomes were obtained by squash of cleaving embryos, previously treated with colchicine and fixed in ethanol-acetic acid 3:1 at 4ºC and stained using the Feulgen reaction. Diplodon chilensis showed a diploid karyotype of 2n = 34, with metacentric and submetacentric chromosomes. This work is the first approach to cytogenetic characterization of Chilean populations of this species.
Tipo: Journal article Palavras-chave: Hyriidae; Diplodon chilensis; Karyotype; Freshwater; Bivalves; Southern Chile; Hyriidae; Diplodon chilensis; Cariotipo; Bivalvos dulceacuícolas; Sur de Chile.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200008
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FISH IMMUNE SYSTEM Gayana
Olabuenaga,Susana E..
The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well...
Tipo: Journal article Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010
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OVIPOSITION BEHAVIOUR IN FOUR SPECIES OF DROSOPHILA Gayana
Alamiri,Zaid.
Oviposition site selection plays a central role in the evolutionary ecology of Drosophila, due to its high relation with the pre-adults fitness since the site selection determines the interactions between the species sharing common resources. Drosophila subobscura Collin which was detected in Chile for the first time in 1978, was found coexisting in the wild, as adults, over apple and raspberry, with D. hydei, D. immigrans and D. melanogaster. It was observed that raspberry has been used only by Drosophila subobscura as a breeding site; meanwhile sharing apple with the others. Our principal purpose was to shed light on the ecological significance of the new species oviposition behaviour; analysing also some of the preference-performance relationship. Two...
Tipo: Journal article Palavras-chave: Oviposition behaviour; D. subobscura; Colonisation; Preference - performance relationship; Substrate physical characters.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200001
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CUATRO ENSAYOS ECOTOXICOLOGICOS PARA EVALUAR LINDANO Y CLORPIRIFOS Gayana
Iannacone,José; Alvariño,Lorena; Caballero,Cecilia; Sánchez,Javier.
La evaluación de riesgos ambientales por productos fitosanitarios es un componente de importancia en los estudios de impacto ambiental en el sector agrícola. Se evaluó la ecotoxicidad de los plaguicidas de suelo, lindano y clorpirifos, sobre la mortalidad del oligoqueto Limnodrilus hoffmeisteri (CL50 24h) y tres plantas, en las cuales se observaron los siguientes efectos subletales: germinación en Lactuca sativa (CI90 96h), crecimiento de raíz en Lycopersicon esculentum (CI50 96h) y clorosis, necrosis, ruptura y formación de hojas nuevas en la lenteja de agua, Lemna minor (CE50 48h). En ambos agroquímicos se observó en general el siguiente orden de ecotoxicidad decreciente: Lycopersicon Lemna Lactuca Limnodrilus. En los tres ensayos con plantas se observó...
Tipo: Journal article Palavras-chave: Ensayo ecotoxicológico; Evaluación de riesgos ambientales; Agroquímico; Lindano; Clorpirifos; Limnodrilus; Lemna; Lycopersicon; Lactuca.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200003
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IXODES URIAE WHITE 1852 (ACARI: IXODIDAE) ASSOCIATED WITH THE PAPUA PENGUIN, PYGOSCELIS PAPUA (FORSTER), IN THE ANTARCTIC PENINSULA Gayana
Casanueva,María E.; Moyano,Hugo I..
Tipo: Journal article
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200012
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MOLUSCOS TERRESTRES DE CHILE. SINONIMIA Y PROBLEMAS RELACIONADOS: 1. FAMILIAS VERONICELLIDAE, PIPILLIDAE Y ACHATINELLIDAE (GASTROPODA: PULMONATA) Gayana
Stuardo,José R.; Vargas-Almonacid,Patricio.
Se examina el status taxonómico de las especies chilenas de las familias Veronicellidae, Pupillidae y Achatinellidae, como parte de una serie de análisis taxonómicos sobre los moluscos terrestres de Chile que se elabora como seguimiento a la sinopsis del grupo publicada con anterioridad (<A HREF="#StuardoyVega">Stuardo &amp; Vega 1985</A>). Son pocos los trabajos trascendentes publicados después de la sinopsis. En la actualidad hay descritas dos especies de Veronicellidae, dos de Pupillidae y 21 especies de Achatinellidae. De éstas, el único registro de Chile para Heterovaginina limayana Lesson es cuestionable y la subespecie Pupoides (Ischnopupoides) minimus costatus Biese, es considerada inválida. Se presenta una exhaustiva compilación de...
Tipo: Journal article Palavras-chave: Mollusca; Sinonimias; Veronicellidae; Pupillidae; Achatinellidae; Neotropical; Oceanía; Chile.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200007
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PREFERENCIA Y MANIPULACION FLORAL DE CENTRIS RODOPHTHALMA PEREZ (HYMENOPTERA: ANTHOPHORIDAE): FLORAL PREFERENCE AND MANIPULATION OF CENTRIS RODOPHTHALMA PEREZ (HYMENOPTERA: ANTHOPHORIDAE) Gayana
Chiappa,Elizabeth; Rodríguez,Sharon; Bascuñán,Rossana.
Se estudió la manipulación y preferencia floral de Centris rodophthalma Pérez en 3 localidades de la IV Región de Chile, caracterizadas, principalmente, por representantes de la familia Fabaceae y condiciones ambientales estrictas que reducen el rango de recursos disponibles para la abeja. Se estimó la influencia de factores florales de 6 especies de plantas en la preferencia de C. rodophthalma, con el objeto de determinar el grado de correspondencia entre la morfología de la abeja y la elección de la flor, considerando: longitud de la lengua de la abeja; tamaño, diseño, color y estructura de la flor; profundidad de la corola; capacidad volumétrica de nectarios y medida del ángulo formado entre nectario y resto del estandarte. De estas variables, sólo las...
Tipo: Journal article Palavras-chave: Centris; Fabaceae; Interacción insecto-planta; Chile.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200002
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CTENARYTAINA EUCALYPTI (MASKELL 1890): THE BLUE GUM PSYLLID IN CHILE (HEMIPTERA: STERNORRYNCHA: PSYLLOIDEA: SPONDYLIASPININAE) Gayana
Olivares,Tania S..
Tipo: Journal article
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200014
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ACTIVIDAD CONTRACTIL DEL MUSCULO PAPILAR CARDIACO Y CONDUCTO DEFERENTE DE RATA INDUCIDA POR VENENO DE LA ARAÑA LATRODECTUS MACTANS DE CHILE Gayana
Romero,Fernando; Altier,Elena; Quiñehua,Carlos; Cayuqueo,Alejandr.
La araña Latrodectus mactans al morder inocula su veneno, el cual induce un cuadro clínico denominado latrodectismo sistémico, del que se desconocen sus efectos en tejidos aislados. Nuestro laboratorio ha obtenido veneno de esta especie de arácnido mediante la técnica de extracción de glándula, lo que permitió iniciar estudios de la reactividad muscular inducida por extracto de glándula y una fracción purificada bioactiva sobre músculo liso, utilizando como modelo preparaciones de músculo aislado papilar cardiaco y conducto deferente de rata. Los resultados muestran que estas toxinas generan un efecto miotónico en el tejido muscular liso del conducto deferente y en el tejido cardiaco inducen inotropismo positivo y aumento de la frecuencia de contracción....
Tipo: Journal article Palavras-chave: Araña viuda negra; Latrodectus mactans de Chile; Músculo cardiaco; Conducto deferente; Contracción.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200006
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UN PARATIPO ERRADO DE PAREUXOA KOEHLERI OLIVARES (LEPIDOPTERA: NOCTUIDAE) Gayana
Olivares,Tania S.; Angulo,Andrés O..
Tipo: Journal article
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200013
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