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| Registros recuperados: 293 | |
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| Liyanagunawardena, Nilukshi; Sivakumar, Thillaiampalam; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Battsetseg, Badgar; Dinh Thi Bich Lan; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki. |
| Babesia bovis is the most virulent Babesia organism, resulting in a high mortality rate in cattle. The genetic diversity of B. bovis merozoite surface antigens (MSAs), such as MSA-1, MSA-2b, and MSA-2c, might be linked to altered immune profiles in the host animals. The present study aimed to develop type-specific PCR assays for Asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in Sri Lanka, Mongolia, and Vietnam. Specific primers were designed for nine Asian msa-1 genotypes, which had been detected based on the phylogeny constructed using msa-1 gene sequences retrieved from the GenBank database. Specificity of the type-specific PCR assays was confirmed using plasmids containing the inserts of msa-1 gene fragments that represent... |
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Palavras-chave: Asia; Babesia bovis; Genetic diversity; Msa-1; Type-specific PCR. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4489 |
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| Tattiyapong, Muncharee; Sivakumar, Thillaiampalam; Takemae, Hitoshi; Simking, Pacharathon; Jittapalapong, Sathaporn; Igarashi, Ikuo; Yokoyama, Naoaki. |
| Babesia bovis, an intraerythrocytic protozoan parasite, causes severe clinical disease in cattle worldwide. The genetic diversity of parasite antigens often results in different immune profiles in infected animals, hindering efforts to develop immune control methodologies against the B. bovis infection. In this study, we analyzed the genetic diversity of the merozoite surface antigen-1 (msa-1) gene using 162 B. bovis-positive blood DNA samples sourced from cattle populations reared in different geographical regions of Thailand. The identity scores shared among 93 msa-1 gene sequences isolated by PCR amplification were 43.5-100%, and the similarity values among the translated amino acid sequences were 42.8-100%. Of 23 total clades detected in our... |
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Palavras-chave: Antigenicity variation; Babesia bovis; Msa-1; Genetic diversity; Thailand. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4490 |
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| Zaabi-Sendi, Sana. |
| The polychaetes communities structure and functioning of the Cap Bon peninsula (Northeast coast of Tunisia), were analyzed from 52 stations from Sidi Daoud to Menzel Horr. The faunistic study reveals the presence of 238 polychaetes species among which 86 records are newly reported for the Tunisian coasts. The total number of polychaetes listed in Tunisia rises to 375 species. The polychaete community structure at the sites was examined by multivariate methods. five main polychaetes communities were identified in the study area, the sandy sediment community (SS), the muddy sediments community (SV), the coarse sediment community (SG), the fine-muddy sediment community (SFV) and the Heterogeneous sediments community (HS). These communities are distributed... |
| Tipo: Theses and Dissertations |
Palavras-chave: Seasonal variations; Genetic diversity; Polychaeta; Annelida. |
| Ano: 2013 |
URL: http://hdl.handle.net/1834/5480 |
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| Agnese, J.F.. |
| Most of the fishes living in the lagoons do not spend all their time in these brackishwaters. Sometimes, they go back to the river to spawn as in the case of freshwater fishes like Chrysichthys nigrodigitatus. Others like marine fishes (e.g., Ethmalosa jimbriata) feed or reproduce in lagoons for a time. Even true lagoon fishes are generally able to live in freshwater and/or in marine water. This is the case for two brackishwater tilapias, Sarotherodon melanotheron and Tilapia guineensis. Both are present in lagoons from Senegal to Congo (formerly Zaire). S. melanotheron is present in marine water near Dakar, Senegal, and in some freshwater lakes in Cote d'Ivoire. 1: guineensis is also present in freshwater in Cote d'Ivoire. |
| Tipo: Proceedings Paper |
Palavras-chave: Lagoon fisheries; Genetic diversity; Brackish water; Lagoon fisheries; Http://aims.fao.org/aos/agrovoc/c_4157. |
| Ano: 2000 |
URL: http://hdl.handle.net/1834/325 |
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| Robainas Barcia, A.. |
| En este trabajo se analiza la variación espacio temporal de los patrones de diversidad genética y estructura poblacional del camarón rosado Farfantepenaeus notialis, en la plataforma Sur de Cuba. Se estudiaron, 22 loci aloenzimáticos, de los cuales seis resultaron polimórficos (Akp3, AmyB, Est3, Gdh, GP7 y Per1); cinco loci microsatélites (PnS01, PnS03, PnS04, PnS18 y PnS20); y un fragmento de 2027pb del ADNmt, en muestras procedentes de ocho localidades del Golfo de Ana María, tres del Golfo de Batabanó y una del Golfo de Guacanayabo, durante los años 1995, 1999 y 2003. El análisis de las poblaciones en el año 1995, empleando marcadores nucleares, reveló diferencias genéticas significativas entre las poblaciones del Golfo de Ana María, como fue... |
| Tipo: Theses and Dissertations |
Palavras-chave: Genetic factors; Genetic diversity; Shrimp culture. |
| Ano: 2004 |
URL: http://hdl.handle.net/1834/5511 |
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| Chaieb, O.. |
| La tortue marine, la caouanne (Caretta caretta), est une espèce en danger d’extinction. Elle est caractérisée par un cycle biologique complexe se basant sur une série de transitions écologiques et géographiques nécessitant des migrations sur de longues distances. En Tunisie, plusieurs études ont été réalisées sur les activités de nidification et les interactions avec les activités de pêche, mais, aucune n’a porté sur l’étude génétique de cette espèce. Dans ce travail, nous nous sommes intéressés à l’analyse génétique de la population nidifiante en Tunisie et des populations des tortues se développant dans les zones néritiques tunisiennes. La population nidifiante a été appréhendée par l’analyse des nouveau-nés échantillonnés durant une période de sept ans... |
| Tipo: Theses and Dissertations |
Palavras-chave: Allozymes; DNA; Genetic diversity; Neritic province. |
| Ano: 2013 |
URL: http://hdl.handle.net/1834/5923 |
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| García-Machado, E.; Chevalier, P.; Solignac, M.. |
| The hamlets are a group of vividly colored fish species of the Serranidae family differentiated only by the color pattern of the body. Although there are divergent views about hamlet taxonomy, experimental and field observations have shown a strong assortative mating, justifying a species status for the different color morphs. Here we analyze the level of evolutionary divergence among six species in respect of mitochondrial DNA, with a view to contrasting the pattern observed with color partitions and previous results obtained with isozymes. The estimated molecular distance among species was low and of the same magnitude as nucleotide diversity within species. Consequently, the net distance and hence the time of divergence between taxa was virtually zero... |
| Tipo: Journal Contribution |
Palavras-chave: MtDNA; Marine fish; Genetic diversity. |
| Ano: 2003 |
URL: http://hdl.handle.net/1834/4569 |
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| Hernández Martínez, D.; Álvarez-Alemán, A.; Bonde, R. K.; Powell, J.A.; García-Machado, E.. |
| El objetivo del presente trabajo fue obtener información preliminar acerca de la composición de haplotipos del ADN mitocóndrico (ADNmt) del manatí (Trichechus manatus) que habita en el archipiélago cubano. Se analizó un total de 13 individuos, 12 de Cuba y uno procedente de la Florida, EU. Las secuencias de un fragmento de 410 pb de la región de control del ADNmt (D-loop) permitieron identificar dos haplotipos. El haplotipo A1, único encontrado en la Florida (incluida la muestra aquí analizada), pero también presente en México, Puerto Rico y República Dominicana. Este haplotipo fue mayoritario (11 de los 12 individuos muestreados en Cuba). El segundo haplotipo fue el A3, referido anteriormente como endémico de Belice, se encontró en un individuo... |
| Tipo: Journal Contribution |
Palavras-chave: Genetic diversity; MtDNA. |
| Ano: 2013 |
URL: http://hdl.handle.net/1834/5252 |
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| Moreira, Juliana Prado; Shimizu, Jarbas Yukio; Sousa, Valderês Aparecida; Moraes, Mario Luis Teixeira de; Moura, Nara Fernandes; Aguiar, Ananda Virgínia de. |
| P. elliotii var. elliottii is the second most planted fast growing tree species in Brazilian subtropics for wood and resin production. The objective of this work was to predict genetic values of fast growing individual trees. A trial was established in a randomized complete blocks design involving 76 treatments, 75 open pollinated progenies from a clonal seed orchard and a commercial progeny as control, in a 3 m x 3 m spacing. Total heights of all individuals were measured at 1, 2, and 3 years old and also stem diameter at 1.3 m height- dbh at 3 years old. Deviance analysis was performed by using Selegen-REML/BLUP software and genetic parameters were estimated. Significant variation was detected among progenies. Estimates of narrow-sense individual... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: REML/BLUP; Genetic improvement; Genetic diversity; Repeatability; Heritability Melhoramento florestal REML/BLUP; Correlações; Diversidade genética; Ganho de seleção; Melhoramento genético. |
| Ano: 2014 |
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/488 |
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| Cabral,Pablo Diego Silva; Soares,Taís Cristina Bastos; Gonçalves,Leandro Simões Azeredo; Amaral Júnior,Antonio Teixeira do; Lima,Andreia Barcelos Passos; Rodrigues,Rosana; Matta,Frederico de Pina. |
| The present work aimed at evaluating the divergence among common bean accessions by their agronomic, morphological and molecular traits, based on the Ward-MLM procedure. A collection of 57 accessions from the gene bank of Universidade Federal do Espírito Santo was used in this study, from which: 31 were landraces belonging to the community Fortaleza, in the municipality of Muqui, ES, Brazil; 20 accessions were provided by Embrapa Trigo; and 6 were commercial cultivars. Five agronomic traits (plant cycle, number of seeds per pod, number of pods per plant, weight of 100 seeds, and grain yield), five morphological traits (growth habit, plant size, seed shape, seed color, and commercial group) and 16 microsatellite primers were evaluated. High genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Genetic diversity; Germplasm characterization; Joint analysis; Molecular markers; Morphological markers. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001000011 |
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| Brige,Felipe Augusto Alves; Celestino,Sonia Maria Costa; Amabile,Renato Fernando; Fagioli,Marcelo; Delvico,Francisco Marcos dos Santos; Montalvão,Ana Paula Leite; Sala,Pedro Ivo Aquino Leite. |
| Abstract: The objective of this work was to quantify the genetic variability of 213 conilon coffee accessions of the Robusta Tropical cultivar, based on chemical characteristics related to the quality of green coffee beans, as well as to identify promising accessions for the breeding program of irrigated conilon coffee in the Brazilian Cerrado. The chemical characteristics evaluated were: protein and caffeine contents, total soluble solids and total lipids, ether extract, pH, and total titratable acidity. The data were subjected to the principal component analysis and cluster analysis based on the similarities observed within the first two principal components using the minimum variance method (Wards) and, as a measure of similarity, the Euclidean... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea canephora; Chemical composition; Genetic diversity; Multivariate analysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102804 |
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| Zucchi,Maria Imaculada; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Couto,Mansuêmia Alves; Morais,Lizz Kezzy de; Vencovsky,Roland. |
| This study was carried out to assess the genetic variability of ten "cagaita" tree (Eugenia dysenterica) populations in Southeastern Goiás. Fifty-four randomly amplified polymorphic DNA (RAPD) loci were used to characterize the population genetic variability, using the analysis of molecular variance (AMOVA). A phiST value of 0.2703 was obtained, showing that 27.03% and 72.97% of the genetic variability is present among and within populations, respectively. The Pearson correlation coefficient (r) among the genetic distances matrix (1 - Jaccard similarity index) and the geographic distances were estimated, and a strong positive correlation was detected. Results suggest that these populations are differentiating through a stochastic process, with restricted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myrtaceae; Cerrado; Tropical species; Genetic diversity. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001000005 |
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| Ribeiro,Carlos Alexandre Gomes; Tanure,Janaína Paula Marques; Maciel,Talles Eduardo Ferreira; Barros,Everaldo Gonçalves de. |
| The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Cultivar protection; Genetic diversity; Genotyping method; Random identity probability. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300005 |
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| Germano,Mariana Gomes; Cannavan,Fabiana de Souza; Mendes,Lucas William; Lima,Amanda Barbosa; Teixeira,Wenceslau Geraldes; Pellizari,Vivian Helena; Tsai,Siu Mui. |
| The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring-hydroxylating dioxygenases (α-ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station - secondary forest (SF) and agriculture (AG) -, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catabolic genes; Genetic diversity; Land use systems; Microbial ecology; Tropical soils. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000500004 |
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| Sayd,Ricardo Meneses; Amabile,Renato Fernando; Faleiro,Fabio Gelape; Bellon,Graciele. |
| The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hordeum vulgare; Genetic diversity; Molecular markers; Quantitative traits.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000200160 |
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| Silveira,Érico Leandro da; Pereira,Rodrigo Matheus; Scaquitto,Denilson César; Pedrinho,Eliamar Aparecida Nascimbém; Val-Moraes,Silvana Pómpeia; Wickert,Ester; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo. |
| Studies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Metagenomic; Microbial communities. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001000008 |
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| Registros recuperados: 293 | |
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