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| Registros recuperados: 978 | |
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| Al-Atiyat,Raed M. |
| Background In sheep breeding, there are situations where relationships recorded at the farm among pedigrees such as parent-offspring, full-sibs or half-sibs need to be tested. A panel of 28 microsatellite (MST) markers was tested to provide accurate pedigree information and resolve the common problem of significant error in pedigree records in Merino sheep. Three different flocks of Australian Merino sheep were investigated. A private farm flock represents a flock with no record availability. Two other flocks were maintained under good managements of full keeping records and being selected for high and low parasite resistances. Results In the studied panel, eight MSTs provided an average of Polymorphic Information content (PIC) equal to 0.65 or more in... |
| Tipo: Journal article |
Palavras-chave: Merino sheep; Paternity verification; Pedigree records; SSR markers. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200009 |
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| Li,Peiqin; Luo,Haiyu; Meng,Jiajia; Sun,Weibo; Wang,Xiaohan; Lu,Shiqiong; Peng,Youliang; Zhou,Ligang. |
| Background Three oligosaccharides (EOS, WOS and SOS) were respectively prepared from the corresponding polysaccharides, namely exopolysaccharide (EPS), water-extracted mycelial polysaccharide (WPS) and sodium hydroxide-extracted mycelial polysaccharides (SPS) from the endophytic fungus Fusarium oxysporum Dzf17. In this study, the effects of EOS, WOS and SOS on the activities of the defense-related enzymes, namely phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD) in its host plant Dioscorea zingiberensis cultures were investigated. Results For the suspension cell cultures of D. zingiberensis, the highest PAL activity was induced by 0.5 mg/mL of WOS at 48 h after treatment, which was 4.55-fold as that of control. Both PPO and... |
| Tipo: Journal article |
Palavras-chave: Endophytic fungus; Phenylalanine ammonia lyase; Peroxidase; Polyphenoloxidase; Polysaccharide. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400003 |
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| Smolik,Milosz. |
| Background: An adaptive mechanism in plant roots is initiated in the event of nitrogen and potassium deficiency, and it facilitates the active uptake of these elements in order to ensure plant growth and survival in stress conditions. Signaling and transduction of signals in response to changing nitrogen and potassium concentrations is a complex process, affected by interactions between various gene expression products, and often subjected to modifications. Results: In order to identify genotypic differences between phenotypes of two populations of recombinant inbred rye lines (153/79-1 x Ot1-3 and Ot0-6 x Ot1-3) in response to nutrition stress caused by nitrogen and potassium deficiency at the seedling stage, bulk segregant analysis was utilized.... |
| Tipo: Journal article |
Palavras-chave: Molecular markers; Nutrient deficiencies; Orthologs; Response; RILs; Rye; TIGR; TOGA. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500001 |
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| Varela,Liliana; Bisang,Roberto. |
| In the 1980s, the technical pattern of production in agriculture changed due to the increasing design of genetically modified plants. Modern biotechnology thrived on events requiring certain thresholds of scientific and technological skills as well as scale economies usually seen in developed countries. The mergers and acquisitions during the mid-1990s led to a world-wide oligopoly composed of very few agri-biotechnological mega-corporations and the literature discusses the impact of the mergers and acquisitions on the agriculture of developing countries with comparative advantages in agriculture. This paper analyzes the world-wide process of agri-biotechnological mega-corporation mergers and acquisitions as well as its impact and interrelationships with... |
| Tipo: Journal article |
Palavras-chave: Internalization; Local adaptation; Mergers and acquisitions; Multinationals. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300010 |
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| Tolmachov,Oleg. |
| Many gene therapy strategies require transfer of high-molecular weight DNA into human cells. To enable clinical trials, these vectors need to be produced on a large scale and at low cost. The production of effective high-capacity vectors like HSV-amplicons and helper-dependent adenoviral vectors is difficult to up-scale, so new inexpensive vectors are needed for the efficient delivery of high-molecular weight DNA to human cells. Bacteriophage lambda vectors can accommodate up to about 46 kb of therapeutic DNA and can be easily produced in an industrial setting. However, the lambda vectors transfer DNA into mammalian cells with only a low efficiency. It was shown that bacteriophage lambda virions ejected their DNA in the presence of the purified receptor... |
| Tipo: Journal article |
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| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400012 |
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| Hakki,Erdogan E; Kayis,Seyit A; Pinarkara,Emine; Sag,Ayla. |
| Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for phytoremediation: however, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 23 different locations of Turkey, and nine hemp type Cannabis accessions, as well as an unknown accession were used. Our interest was to identify the genetic relatedness of the seized samples and to separate drug and hemp type plants.... |
| Tipo: Journal article |
Palavras-chave: Cluster analysis; Hemp; ISSR; Marijuana; Principle coordinate analysis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400010 |
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| Lawrence,Paulraj K.; Koundal,Kripa Ram. |
| Plant proteinase inhibitors (PIs) have been well established to play a potent defensive role against predators and pathogens. Although diverse endogenous functions for these proteins has been proposed, ranging from regulators of endogenous proteinases to act as storage proteins, evidence for many of these roles is partial, or confined to isolated examples. On the other hand, many PIs have been shown to act as defensive compounds against pests by direct assay or by expression in transgenic crop plants, and a body of evidence for their role in plant defense has been accumulated consistently. The role and mechanism of action for most of these inhibitors are being studied in detail and their respective genes isolated. These genes have been used for the... |
| Tipo: Journal article |
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| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100014 |
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| Sun,Chuan-Bao; Kong,Qiu-Lian; Xu,Wen-Si. |
| The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts. |
| Tipo: Journal article |
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| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100009 |
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| Cauerhff,Ana; Castro,Guillermo R. |
| Background: In the past decade, considerable attention has been paid for the development of novel strategies for the synthesis of different kind of nano-objects. Most of the current strategies are usually working by the use physical or chemical principles to develop a myriad of nano-objects with multiple applications. Main fields of nanotechnology applications range from catalysis, micro- and nano-electronics (semiconductors, single electrons transistors), non-linear optic devices, photo-electrochemistry to biomedicine, diagnostics, foods and environment, chemical analysis and others. Results: Two main avenues for nanoparticles synthesis: cell-free extract and cell cultivation have been reported. The state of art of both biotechnological approaches for... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Bionanoparticles; Fermentation; Fungi green chemistry; Plant extracts. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300011 |
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| Mendoza-Macedo,Karina; Romero-Díaz,Alexis J; Miranda-Hernández,Mariana P; Campos-García,Víctor R; Ramírez-Ibañez,Nancy D; Juárez-Bayardo,L. Carmina; Moreno-Duran,Karen; Cedillo-Robles,Miriam S; Pérez,Nestor O; Jung-Cook,Helgi; Flores-Ortiz,Luis F; Medina-Rivero,Emilio. |
| Background: Developing countries have an estimate of ten times more approved biosimilars than developed countries. This disparity demands the need of an objective regulation that incorporates health policies according to the technological and economical capabilities of each country. One of the challenges lies on the establishment of comparability principles based on a physicochemical and biological characterization that should determine the extent of additional non-clinical and clinical studies. This is particularly relevant for licensed biosimilars in developing countries, which have an extensive clinical experience since their approval as generics' in some cases more than a decade. To exemplify the current status of biosimilars in Mexico' a... |
| Tipo: Journal article |
Palavras-chave: Bio therapeutic; Comparability; Critical quality attributes; Developing countries; Generics; Health policies; High quality biosimilars; Regulation. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600010 |
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| Johnson,Elizabeth; Herd,Susanna; Tisdall,Judith. |
| Generic skills are skills that are common to all graduates rather than specific for a particular field of study. They include communication and problem-solving skills and the ability to work successfully in teams. To produce graduates with these skills, university courses must include exercises that encourage development of these skills and assessment procedures which reward achievement in these areas. This paper describes three simple exercises designed to develop generic skills that can easily be incorporated into existing courses. |
| Tipo: Journal article |
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| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200004 |
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| Guebel,Daniel V.; Torres Darias,Néstor V.. |
| Idiophase, the citric acid producing stage of Aspergillus niger was mathematically modeled to identify required genetic manipulations to optimize citric acid production rate. For this reason, a consistent picture of cell functioning had to be achieved. The transient idiophase nature was established by stoichiometric analysis. The main intracellular fluxes were computed by application of material and physiological constraints (ATP, reduction equivalents, proton motive force) at culture time 120 hours. The HMP pathway accounts for 16% of the glucose input (carbon basis), the Krebs cycle for 13% and the citric acid synthesis for the remaining 71%. This profile implies an operative glycerol-P shuttle. It recycles 93% of the cytosolic glycerol-P to cytosolic... |
| Tipo: Journal article |
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| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100001 |
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| Sybesma,Wilbert; Hugenholtz,Jeroen; de Vos,Willem M.; Smid,Eddy J.. |
| Within the European Union (EU), the use of genetically modified organisms (GMOs) in food production is not widely applied and accepted. In contrast to the United States of America, the current EU legislation limits the introduction of functional foods derived from GMOs that may bring a clear benefit to the consumer. Genetically modified lactic acid bacteria (GM-LAB) can be considered as a different class of GMOs, and the European Union is preparing regulations for the risk assessment of genetically modified microorganisms. Since these procedures are not yet implemented, the current risk assessment procedure is shared for GMOs derived from micro organisms, plants, or animals. At present, the use of organisms in food production that have uncontrolled genetic... |
| Tipo: Journal article |
Palavras-chave: Lactic acid bacteria; Genetic modification; Legislation; Novel foods; Safety assessment. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400011 |
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| Registros recuperados: 978 | |
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